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[DOC File]EXERCISE 1 - BrainMass
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Step 3: Add 1 ml from step 2 to 9 ml diluent and mix (1:10) Each dilution in this series is a 1:10 dilution or 10-1. Final dilution (product of all dilutions) = 1/10 x 1/10 x 1/10 = 1/1000 (1:1000 or 1 x 10-3) b. The dilution factor is the reciprocal of the final dilution. For the above example, the dilution factor is 1000 or 103
1 200 dilution lab chart

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First serial dilution plate: 60 microliters from each stock plate well is serially diluted (100% DMSO) by column to make the 96-well primary 'Mother plate'. (columns 1 and 12 are left empty). Serial dilution 1:3 with transfer 40 microliters from High Concentration to Column 10 (9 concentrations). Column 11 is 100% DMSO.
1 20 dilution example

[DOC File]Discover the Microbes Within - University of Rochester
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DNA Elution and Dilution. Label 5-1.5 ml Eppendorf tubes with 1-5 and your initials. These will contain your purified DNA samples. Place the spin column into a clean 1.5 ml tube and pipet 200 microliters of Buffer AE directly onto the membrane. Incubate at room temperature for 1 minute. Centrifuge for 1 minute to elute.
1 to 20 dilution microliters

[DOC File]Urine Cultures Bacterial Identification Microbiology ...
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The results are reported in cells/mL. For example, if 0.001 mL (1 microliter, abbreviated as 1 μl) is plated, the colony number must be multiplied by 1000, since 0.001 mL is 1/1000th of a mL. This serial dilution technique is used when the investigator has …
dilution equation

[DOC File]VWT 172: Laboratory Analysis of Musts and Wines
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Estimate % residual sugar from chart (% approx. = g/100 mL; for g/L multiply by 10) If color goes past orange to brown the sample is too sweet and must be diluted before retesting. For samples of 1 to 5% sugar, use 0.1 mL (2 drops) of wine and 0.4 mL (8 drops) of water. Multiply % sugar reading from color chart x5 to compensate for dilution
how to calculate dilutions

[DOCX File]TABLE OF CONTENTS - University of Kentucky
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Do this by pipetting 9 µl of sterile water into each of 5 microfuge tubes. Your stock DNA is at a concentration of 100 ng/ µl. Transfer 1 µl of the stock DNA to the first dilution tube mix and label this tube 10 ng/ µl. Next transfer 1 µl of the 10 ng/ µl DNA to the next dilution …
1 40 dilution

[DOC File]DILUTIONS AND STANDARDS
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1. "Dilution: - Dilutions are expressed as the ratio of the quantity of a desired solute (serum, urine, chemical solution, etc.) contained in a solvent (diluent). A 1:10 dilution of serum was made by adding one part serum to nine parts diluent to make a total of ten parts. If 1.0 milliliter of serum is added to 9.0 milliliters of H20, a total ...
how to make a 1 100 dilution

[DOC File]Discover the Microbes Within
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DNA Elution and Dilution. Pipet 100 µl of Buffer AE directly onto the membrane. This is an elution buffer that rinses the DNA off the spin column filter and into the 1.5 ml tube. Incubate at room temperature for 1 minute. Centrifuge for 1 minute to elute. Make sure that the open lids of the 1.5 ml tubes don’t interfere with the centrifugation.
1 to 20 dilution

[DOC File]BITC1311 Introduction to Biotechnology
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Microliters and milliliters Grams and milligrams Days and weeks ... 1.100 g 1100 g Calculations Using Significant Figures. ... In dilutions, parts can be of any unit. If you combine 1 mL food coloring with 1mL water, you are using the same dilution factor (1:1, or ½) as the person who combines 1 ounce of food coloring with 1 ounce of water. If ...
1 200 dilution lab chart

[DOC File]Bergen
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Next, make a 1:100 dilution of the oil using the diluents provided. With a new pipette tip, take 100µL of “B” and place it into a microcentrifuge tube labeled “C” and with a new pipette tip add 900 µL of diluent. Mix by flicking with your finger. Next, make a 1:1000 dilution of the oil using the diluents provided.
1 20 dilution example

1 100 dilution chart microliters