1 20 dilution in microliters
[DOC File]CE 742 Advanced Topics in Environmental Engineering Lab …
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Make a 1:100 dilution of your DNA extract, with a final volume of 500 microliters (eg. 5 microliters of DNA extract added to 495 microliters of D.I. water). You can do this in a microcentrifuge tube. You may use regular D.I. water to do the dilution (it is not necessary to use the ultrapure water, since you will throw away this sample after ...
[DOC File]Discover the Microbes Within
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DNA Elution and Dilution. Label 5-1.5 ml Eppendorf tubes with 1-5 and your initials. These will contain your purified DNA samples. Place the spin column into a clean 1.5 ml tube and pipet 200 microliters of Buffer AE directly onto the membrane. Incubate at room temperature for 1 minute. Centrifuge for 1 minute to elute.
[DOC File]Laboratory: _________________________________________ Date ...
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Contrived nasal swab samples were prepared by absorbing 20 microliters of each virus dilution onto the swab. The contrived swab samples were tested according to the test procedure. The LOD was determined as the lowest virus concentration that was detected ≥ 95% of the time (i.e., concentration at which at least 19 out of 20 replicates tested ...
[DOC File]ELISA - Biomanufacturing
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5.1.5 1:100 dilution of Goat anti-rabbit IgG - Peroxidase - Add 20 µl of Goat anti-rabbit IgG - Peroxidase to 1980 µl (Note! Microliters!) of TBS buffer. 5.1.6 1:400 dilution of Goat anti-rabbit IgG – Peroxidase - Add 500 µl of the dilution 1:100 of Goat anti-rabbit IgG - …
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First serial dilution plate: 60 microliters from each stock plate well is serially diluted (100% DMSO) by column to make the 96-well primary 'Mother plate'. (columns 1 and 12 are left empty). Serial dilution 1:3 with transfer 40 microliters from High Concentration to Column 10 (9 concentrations). Column 11 is 100% DMSO.
[DOC File]Genetic Reconstruction (“Gene Gorging”) Experiment Protocol
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Also, as a positive control, dilute 10 microliters from one tube into 0.9 mL of saline (1:100 dilution) and plate 50 microliters of this on a TA plate. As a negative control, if you have a spare tube of competent cells, thaw on ice, dilute 10 microliters into 0.9 mL of saline (1:100 dilution), and plate 50 microliters on a Cam+Kan LB plates.
[DOC File]DILUTIONS AND STANDARDS
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A serum specimen was diluted 1:20 and then 2 mL of that was diluted to 10mL, and 1 mL of that was added to 4 mL using H20. Exactly 2 mL of this last dilution was discarded, and the remainder was diluted to 30 mL. What was the final dilution of the serum specimen? Read each portion carefully. 1/20 x 2/10 x 1/5 x 3/30 = 6/30,000 = 1/5,000 or 1:5000
[DOC File]EXERCISE 1 - BrainMass
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The scales on micropipettors are in microliters (µl). One microliter is one thousandth of a milliliter (1 µl = 0.001 ml). ... Each dilution in this series is a 1:10 dilution or 10-1. Final dilution (product of all dilutions) = 1/10 x 1/10 x 1/10 ... Place the sample tubes in a 37oC water bath for 20 minutes. Add 2.8 ml of 1.0 M Na2CO3 to each ...
[DOC File]Urine Cultures Bacterial Identification Microbiology ...
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The results are reported in cells/mL. For example, if 0.001 mL (1 microliter, abbreviated as 1 μl) is plated, the colony number must be multiplied by 1000, since 0.001 mL is 1/1000th of a mL. This serial dilution technique is used when the investigator has …
[DOCX File]ExpansionMicroscopy.org
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Aliquot this stock solution in 20 uL aliquots, and store in a desiccated environment at -20 C. Adjust AcX to a concentration of 0.03-0.1 mg/ml (that is, 0.03 mg/ml for samples fixed with non-aldehyde fixatives; 0.1 mg/ml for samples fixed with aldehyde fixatives) in 1x PBS.
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