1 to 200 dilution
5C-6
Titer less than incomplete in the 1:50 dilution shall be considered negative. (b) Official vaccinates. Blood titer showing complete reaction in the 1:200 dilution or higher shall be classed as a reactor. Titer showing any reaction in the 1:100 dilution or incomplete in the 1:200 dilution …
[DOC File]Title: Dilution Experiment
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P-200, P-20 Pipetman Dilution of a DNA Stock. Label 3 microcentrifuge tubes as dilution tubes number 1, 2 and 3. Pipet 90 µl of TE buffer solution into each microcentrifuge tube. (Use the P-200 pipetman) Pipet 10 µl of your DNA stock solution into each microcentrifuge tube. (Use the P-20 pipetman)
[DOC File]DILUTIONS AND STANDARDS
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1/20 x 2/10 x 1/5 x 3/30 = 6/30,000 = 1/5,000 or 1:5000 Always reduce the final answer to a fraction with the lowest possible denominator. In the Immunology or Serology area serial dilutions, sometimes referred to as serological dilutions, are extensively used in the test procedures.
[DOC File]MERCY MEDICAL CENTER
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1 square - multiply by 200 . 5 small squares - multiply by 1000. For very cloudy or bloody fluids requiring a final dilution of 1/200. Dilute specimen 1:100 with saline or Isoton (0.1 ml of specimen to 9.9 diluent). From the above dilution, make an additional dilution 1:2 using the appropriate diluting reagent (0.2 ml to 0.2 diluting fluid).
[DOC File]A1.1.5.ELISA
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Under the diagram, calculate the dilution in each well. Think about what you have added to each well and about the practice serial dilution questions you completed in Part II. Note that the concentration of antigen in well #1 is 1000 ng/ml. Calculate the concentration of antigen in wells 2-12. Show your work on your sheet of paper.
Annals of the Rheumatic Diseases
In brief, samples were stained using a mouse anti-neutrophil elastase (NE) mAb (1/200 dilution; Santa Cruz Biotechnology), a rabbit anti-citrullinated H3 (R2+R8+R17) (1/200 dilution; Abcam) polyclonal Ab, a mouse anti-myeloperoxidase (MPO)-specific mAb (1/200 dilution; DAKO), a mouse anti-TF mAb (1/200 dilution; Sekisui Diagnostics), a mouse ...
[DOC File]Isolation of Human PBMC from Whole Blood
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Make 1:200 dilution in CyPBS of Ir-intercalator. Add 20 uL of diluted Ir-intercalator solution to each sample, pipet to mix. Incubate on ice for 20 minutes. Wash x 3 in MilliQ water . Centrifuge cells at 2000rpm for 8 minutes at 4 (C. Aspirate. Acquire samples …
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