1 to 500 dilution
[DOC File]Dilution Worksheet( Homework) Mrs
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What volume is needed to make 2.00 L of 1.00 M solution? 5. A 0.500 M solution is to be diluted to 500.0 mL of a 0.150 M solution. How many mL of the 0.500 M solution are required? 6. A stock solution of 10.0 M NaOH is prepared. From this solution, you need to make 250.0 mL of 0.375 M solution. ... Dilution Worksheet( Homework) Mrs ...
[DOC File]Making Dilutions Worksheet
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Using a 4.0 M solution of MgSO4 , determine how to make 300 mL of a 1.7 M dilution. If you dilute 174 mL of a 1.6 M solution of LiCl to 1.0 L, determine the new concentration of the solution. One liter of a solution is prepared by dissolving 125.6 g of NaF in it. If I took 180. mL of that solution and diluted it to 500. mL, determine the ...
[DOC File]DILUTIONS AND STANDARDS
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A 1:10 dilution of serum was made by adding one part serum to nine parts diluent to make a total of ten parts. If 1.0 milliliter of serum is added to 9.0 milliliters of H20, a total volume of 10.0 milliliters is obtained. Therefore, the dilution is expressed according to the following equation.
[DOC File]Maxi-prep Overall Procedure (by Steven Chang)
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1. Next to a fire, put 5mL LB with the appropriate amount of antibiotics in a round bottom tube. (For ampicillin, use 1:1000 dilution i.e. 5μL. For kanamycin, use 1:500 dilution i.e. 10μL.) 2. Pick one medium sized colony by using a 10 or 200μL pipette tip to touch the bacteria colony and put the tip into the prepared LB media.
[DOC File]Bio-rad Protein Assay: Bradford
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Add 5µl cell lysates (varies according to protein concentration) into 995µl 1:5 diluted Bio-Rad reagent (dilution factor 1:200) and incubate at room temperature for 5 minutes and read absorbance at 595nm. Save standard curve: In theory, you need to do standard curve every time.
[DOC File]CHEMISTRY 11 SOLUTIONS WORKSHEET #2
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12) 75.0 mL of 1.00 M LiNO3 and 115 mL of 2.50 M FeCl3 C) Some chemicals come from suppliers as highly concentrated solutions. In order to use them properly (and safely) they need to be diluted.
[DOC File]Solutions and Dilutions
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A 2-fold dilution is one part or mL of the stock solution with one part or 1 mL of diluent. For Dilutions Only: Using the algebraic formula below, one can calculate the volume, V1, of the initial stock solution or C1 to give the final working or secondary stock concentration, C2, and final working or …
[DOC File]Determination of Biochemical Oxygen Demand (BOD5)
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Add 1 ml or each nutrient solution per liter of dilution water. The phosphate buffer is the critical nutrient in stimulating contaminating growths so it must be added the day the water is to be used. Distilled water should be allowed to equilibrate in the incubator or with outside air for at …
[DOC File]Sample Problems:
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ml of 1 : 500 needed = 4.8 X 500 / 1 = 2400 ml. X- Dilution & Concentration of Solutions: There are 2 important rules to follow: When ratio strengths are given in the problem, convert to percentage (%) strengths before beginning your calculations. Whenever the problem deals with proportion parts,
[DOC File]Chapter 1 - Solutions
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2nd dilution Md = (10.00 mL/250.0 mL) (2.079 x 10-5 M) = 8.32 x 10-7 mol/L This is the molarity of the final solution b) Calculate the mass of KMnO4 needed to prepare the final solution if …
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