Abi sequencer applied biosystems
[DOCX File]University of Maryland School of Medicine
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4.5Applied Biosystems 3730XL high-throughput 96-capillary DNA sequencer and ABI software and GeneMapper® software (for allele sizing) (Applied Biosystems). 4.6Combi Thermosealer (ABGene; …
[DOC File]Hey Wee, - Garvan
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Amplification was performed in a 2700 Applied Biosystems thermal cycler through a denaturation step of 3 minutes at 94°C and 25 cycles consisting of 10 seconds at 94°C and 3 minutes at 68°C. Samples were analyzed on an ABI 3100 DNA Sequencer (Applied Biosystems).
[DOCX File]Materials and Equipment
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Amplified fragments are then analyzed by capillary electrophoresis on an automated DNA sequencer and sized using suitable software. This protocol has been developed for the Applied Biosystems (ABI) …
[DOCX File]Griffiths, A.M.,
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Amplification products were electrophoresed using an ABI 377 automated sequencer (Applied Biosystems Inc., Foster City, CA). Gel images were analyzed in Genescan v 3.1.2 (Applied Biosystems) and alleles were scored with Genotyper v 2.5 (Applied Biosystems).
[DOC File]Page 1 of 9 : Home
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100 (Applied Biosystems). Loci from seventy individuals were genotyped on ABI PRISM 101 3130xl sequencer (Applied Biosystems). Allele sizes were determined with Genescan 600- 102 LIZ internal size standard and Genemapper 4.0 software (Applied Biosystems…
[DOC File]Here’s the Title1 - UCANR
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We mixed 0.75 (l of PCR product with 8 (l of formamide and 0.5 (l of 500 LIZ size standard (Applied Biosystems, Foster City, CA) and we electrophoresed this cocktail on an ABI 3730 automated sequencer (Applied Biosystems, Foster City, CA). We used GENESCAN 3.7 and GENOTYPER 3.7 (Applied Biosystems, Foster City, CA) to analyze ABI …
[DOC File]Clinical and Immunologic Response from the …
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For each patient, the reverse transcriptase and protease portions of the pol gene were amplified by RT-PCR, 5 clones were produced, plasmid DNA extracted, and complete sequencing was performed by an ABI sequencer (Applied Biosystems…
[DOCX File]Harvard University
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“Sanger Sequencing” Sanger DNA Sequencing (Image of ABI) Brand Name underneath. Applied Biosystems 3730xl Sequencer. Our DNA Sequencing division. functions both as a small-scale …
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