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Actin isoform compartments in chicken gizzard smooth ...
-sm actin and sm-myosin antibodies, -sm Tm is a valuable tool for the study of smooth muscle. (J Histochem Cytochem 53:875–883, 2005) S mooth muscle controls the contraction and tone of large and small blood vessels, airways, gut, and stom-ach. It is a complex structure composed of many dif-ferent proteins including smooth-muscle-specific iso-forms of actin and myosin, calponin, caldesmon ...
anti smooth muscle antibodies cpt

Frequency and significance of antibodies to actin in type ...
clone secreting a-smooth muscle actin-speciflc antibodies (anti-ctsm-1) was obtained after a precloning and two cionings by limiting dilution. The other bybridomas were transferred to a 24-well culture plate (Falcon Labware) and grown to confluence before being frozen in hypoxanthine/thymidine medium containing 20% FCS and 10% DMSO. ELISAs were performed on plates coated with either platelet ...
actin smooth muscle igg

BOND Ready-to-Use Primary Antibody Smooth Muscle Actin ...
A negative result for anti-F-actin antibodies does not exclude a diagnosis of autoimmune hepatitis (AIH). Supportive Data In a study performed at Mayo Clinic, 173 serum samples submitted for clinical testing for anti-smooth muscle antibodies (anti-SMA), as performed by indirect immunofluorescence, were collected. These samples were subsequently tested using the anti-filamentous-actin (F-actin ...
actin antibody level

Smooth Muscle-specific Tropomyosin Is a Marker of Fully ...
Histone deacetylase HDAC8 associates with smooth muscle α-actin and is essential for smooth muscle cell ... and anti-β-actin (clone AC-15) monoclonal antibodies (mAb) were from Sigma-Aldrich (Bornem, Belgium). Anti-α-SMA (clone 1A4) antibody, immunoglobulin G1 (IgG1; clone DAK-GO1), and IgG2a (clone DAK-GO5) were obtained from Dako (Glostrup, Denmark). Goat anti-rabbit IgG, rabbit …
actin antibody test

[PDF File]A Monoclonal Antibody against a-Smooth Muscle Actin: A New ...
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Smooth Muscle Actin (alpha sm-1) primary antibody is IHC Protocol F. No pretreatment is recommended. Results Expected Normal Tissues αsm-1 αsm-1 detected alpha smooth muscle actin in the cytoplasm of smooth muscle cells. These cells can be found in vascular walls, intestinal muscularis mucosae and muscularis propria and in the stroma of various tissues. It also reacted with …
actin immunoglobulin g test

Histone deacetylase HDAC8 associates with smooth muscle α ...
Theaffinity-purifiedantibodies to smooth muscle myosin kinase wereused to study the location ofmyosinkinasein avarietyofnonmusclecells. Immunofluores- cence studies indicate that myosin light chain kinase is localized on microfilament bundles (stress fibers) in cultured fibroblasts. Thestress fiberstainingpatternis abolishedwhentheantibodies areincubatedwithpurified smoothmusclemyosinlight ...
what is actin antibody

[PDF File]Autoimmune Hepatitis Diagnostic Panel
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The increased smooth muscle actin may affect force production and further demonstrates the plasticity of smooth muscle cells in the inflamed intestine [1]. Studies also show that human intestinal smooth muscle cells respond to IL-1beta and TNF-alpha stimulation by releasing IL-6, which may significantly contribute to the overall systemic inflammatory response [2]. The colonic smooth muscle ...
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Smooth Muscle Antibody (SMA) and F-actin Antibody
rior to testing for smooth muscle antibodies (SMA); 2) if ficity for autoimmune liver disease,12-14 and they have been these antibodies identify patients with distinctive clini-advocated as better markers for autoimmune hepatitis than cal features; and 3) if the production of antibodies to SMA.4,15,16 Indeed, the designation, ‘‘anti-actin hepatitis,’’ has actin is associated with ...
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F-Actin Ab, IgG, S Overview
smooth muscle, speciﬁc antibodies have been used to demon-strate subcellular sorting of isoactins (Herman, 1993), but the localisation of actin isoforms in smooth muscle in vivo has yet to be established. We demonstrate here that an antibody directed against β- cytoplasmic actin speciﬁcally labels the cytoskeletal compart-ment of smooth muscle cells. In contrast, an antibody that cross ...
anti smooth muscle antibodies cpt

[PDF File]Characterization of antibodies to smooth muscle myosin ...
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The AIH Diagnostic Panel includes tests for actin (smooth muscle) antibody, antinuclear antibodies (ANAs), and liver/ kidney microsome antibody (LKM-1). ANAs and actin antibody are associated with type 1 AIH, the most common form in adults in the United States, while LKM-1 antibody is associated with type 2 AIH, which is more commonly found in children.2 The panel also includes mitochondrial ...
actin smooth muscle igg

Actin smooth muscle antibodies