Adenovirus isolation type

    • Molecular Epidemiology of Adenovirus Type 21 Respiratory Strains ...

      Genomic Analysis of Select Human Adenovirus Type 21 Strains Strain Designation Isolation Site Isolation Year REA Variant/Genome Type GenBank Accession# AV-1645 Saudi Arabia 1956 21p AY601633 ... adenovirus type 21; LRTI, lower respiratory tract infection; MCRD, Marine Corps Recruit Depot; NHRC, Naval Health Research Center; NRTC, Naval Recruit ...


    • [PDF File]Isolation and identification of canine adenovirus type 2 from a ...

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      Abstract: Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The ...


    • ISOLATION OF FOWL ADENOVIRUS IN CHICKEN EMBRYO LIVER CELL CULTURE AND ...

      Isolation of the virus is an important and essential procedure for the subsequent identification and typing of the virus. The fowl adenovirus can be grown in primary cell cultures of chicken kidney (Khaweja et al., 1988) or chicken embryonic liver (CEL) cells (Naeem et al., 1995). CEL culture was used for the isolation of FAVs taken


    • [PDF File]Epidemic Spread of Adenovirus Type 4-Associated Acute Respiratory ...

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      The adenovirus type 4 outbreak in the advanced training population at Fort Gordon differed from the much larger outbreak in Fort Jackson basic trainees. At Fort Jackson, adenovirus type 4 accounted for more than 90% of ARD hospitalizations toward the end of the 1997 epidemic (3). At Fort Gordon, isolation of adenovirus type 4 occurred less ...


    • [PDF File]ORGANISM &/or CONDITION PRECAUTION TYPE Guide to isolation duration ...

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      Adenovirus infection - respiratory Contact & Droplet Duration of hospitalization. For extended hospitalization or extenuating circumstances, ... isolation. Hepatitis, viral - Type B - HBsAg positive Standard See specific recommendation for hemodialysis pt Hepatitis, viral - Type C and other unspecified non-A, non-B Standard See specific ...


    • [PDF File]Molecular characterization of a bovine adenovirus type 7 (Bovine ...

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      closely antigenically related to ovine Adenovirus type 7 [6, 12, 19]. However, the complete genome sequences of the “Fukuroi” strain and the derivate live attenuated vac-cine strain “TS-GT were only recently reported [20]. 18, is is augmented by a complete sequence for the SD18-74 strain which was isolated from pooled lung tissue


    • Biochemical Analysis of Adenovirus Type 5 DNA-binding Protein Mutants

      We previously reported the isolation and functional characterization of seven adenovirus type 5 (Ad5) DNA-binding protein (DBP) point mutants (Quinn, C. O., and Kitchingman, G. R. (1986) J. Virol. 60, 653- 661). Six of the seven mutants were defective in their ability to help adeno-associated virus replicate its DNA.


    • Isolation and evaluation of the immunizing subunits of bovine ...

      part x. isolation and evaluation of the immunizing subunits of bovine adenovirus types 2, 3 and 7 u abstract 16 introduction 17 materials and methods 19 results 34 discussion 59 acknowledgements 62 references 63 part xi. genomic homology between bovine adenovirus type 7 and human adenovirus type 2 67 abstract 69 introduction 70



    • [PDF File]Isolation and genetic characterization of canine adenovirus type 2 from ...

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      Canine adenovirus (CAV), a non-enveloped dsDNA virus, is a member of the genus Mastadenovirus under the fam-ily Adenoviridae. Based on genetic and antigenic charac-teristics, canine adenoviruses are divided into two types: canine adenovirus type 1 (CAV-1) and canine adenovirus type 2 (CAV-2) [1 ]. Although CAV-1 aects the digestive


    • [PDF File]Isolation and Characterization of Different Fowl Adenovirus Types ...

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      FAdV Type 4 vaccine. A line of precipitation was observed in 3 of the samples (Fig. 4) indicating FAdV type 4 infection. PCR and RFLP analysis Hexon gene of fowl adenovirus was amplified from viral DNA to obtain 897 bp PCR product which was resolved on 1.8% agarose gel (Fig.5). Out of 48 samples collected from the


    • [PDF File]Isolation ofadenovirus type 19 from the male and female genital tracts

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      infect mucous membranes, and adenovirus type 11 has been isolated from the urine of children with haemorrhagic cystitis,5 we have found only one previous account of adenovirus isolation from the genital tract. Laverty et a16 reported the isolation of adenovirus type 19 fromthe cervix ofawomanwith conjunctivitis, sore throat, cervical lympha-


    • Isolation of adenovirus from lambs with upper respiratory syndrome

      type-3 virus (PI-3). Virus isolation studies were performed on nasal secretions samples taken at the same time. Parainfluenza type-3 was isolated from 1 of 275 lambs tested, and there was 2.5% overall 4-fold increase in antibody titer to PI-3 during the 2-year study. An adenovirus with a different restriction endonuclease digestion


    • Isolation and evaluation of the immunizing subunits of bovine ...

      Isolation and evaluation of the immunising aubunita of bovine adenovirua typea 2, 3 and 7 by Jane Kwun-Lai Kan Battlea A Diaaertation Submitted to the Graduate Faculty in Partial Fulfillment of the Requirementa for the Degree of DOCTOR OF FHllOSOPHY Interdepartmental Program: lomunobiology Maj or: Xmmunobiology In Charge of Ma^or work


    • Isolation of Temperature-sensitive Mutants of Adenovirus Type 5

      Nitrous acid inactivation of adenovirus type 5. Isolation of mutants Mutagenized stocks were appropriately diluted and plated for plaques on HeLa cell monolayers at 3 I°. After some I5 to I8 days incubation, well-isolated plaques were picked at random from the cultures, frozen and thawed, and retitrated at 3I ° and 38°. ...


    • [PDF File]Isolation Characterization of an Extremely Basic Protein Adenovirus Type

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      argininyl residues, adenovirus type 5 was found to contain two minor basic polypeptides of extreme cathodic mobility in addition to the two known core proteins. Thefastest-migrating polypeptide, named g protein, and the second-fastestpolypeptide arefoundinadenovirionsandvirus-infected KBcells butnot in top componentsor in uninfected cells ...


    • Isolation and Epidemiology of Falcon Adenovirus

      few previous reports of adenovirus infections of falcons (4, 10, 14), the source of infection was not determined. Forbes et al. (4) hypothesized that the agent was a poultry virus and that exposure of falcons occurred through food sources. This was based on isolation of an adenovirus from the chickens and turkeys used to feed the falcons.


    • Isolation and properties of adenovirus type 2 proteinase.

      We have cloned and expressed the human adenovirus type 2 proteinase gene in Escherichia coli. The ex- pressed proteinase was isolated by a four-step chro- matographic procedure. Purity and identity of the re- combinant protein was established by two-dimensional gel electrophoresis, N-terminal sequencing, and spe- cific antisera.


    • [PDF File]Adenovirus: A common cause of respiratory illness - Centers for Disease ...

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      personnel who may be at higher risk for infection from these adenovirus types. The safety and effectiveness of this vaccine has not been studied in the general population or in people with weakened immune systems, and it is not approved for use outside of the military. There is currently no adenovirus vaccine available for the general public.


    • Adenovirus Isolation and Serology from Wild Bobwhite Quail ... - JSTOR

      QBV virus in CKC cultures. Avian adenovirus may grow preferen-tially in a homologous culture system; therefore, the sensitivity of the isolation system may have been improved by using quail cell culture (9). The absence of antibody to the TR-59 type virus isolated in this study cannot be fully evaluated, since serum samples were


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