Agrobacterium mediated transformation ppt
[DOC File]Research and Development
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Presentation at MAFF Crop Mol Genetics Meeting: Agrobacterium-mediated transformation of cereals February 2000. London Kuai B, Wan S.M., Perret S, Dalton S.J. Bettany A.J.E and Morris P. (2000) Production of fertile transgenic oat (Avena sativa L.) plants and stability of bar gene expression in T1 and T2 inbred progeny.
[DOCX File]A1106-AppR-SD1
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Agrobacterium-mediated transformation and . contains four expression cassettes: Comprehensive molecular analyses of line 4114 indicate there is a single insertion site comprising a single, complete copy of each of the . cry1F, cry34Ab1, cry35Ab1, and. pat . genes. The introduced genetic elements are stably inherited from one generation to the next.
[DOC File]Edible Vaccines: A study of the Norwalk virus capsid ...
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Edible Vaccines: A study of the Norwalk virus capsid protein (NVCP) in potatoes and tobacco. Jane Doe, November 4, 200X. In an age where there is the medical knowledge to vaccinate children and ward of life-threatening diseases there are an estimated 900,000 children in the US and millions in developing nations that still remain unvaccinated today.
[DOC File]ATTACHMENT 2
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Cotton lines MXB-7 and MXB-9 were both produced by Agrobacterium-mediated transformation of Gossypium hirsutum L. GC510, using the transformation vectors pMYC3006 and pAGM281 respectively. The plasmid pMYC3006 contains the cry1Ac and pat genes, and the plasmid pAGM281 contains the cry1F and pat genes (see Table 1).
[DOC File]SAFETY ASSESSMENT REPORT - Food Standards
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Agrobacterium mediated transformation involves incubation of the bacteria carrying the particular plasmid with plant cells for a few hours to days, during which time T-DNA transfer takes place. The cells were then washed and cultured in the presence of the selection agent, and …
[DOCX File]Wang MB, Wesley V, Finnegan EJ, Smith NA and Waterhouse …
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Agrobacterium tumefaciens-mediated transformation of monocot plants. Acta Horticulturae. 461: 401-407 (times cited: 6. Upadhyaya NM, Li ZY, Ramm K, Gaudron J, Wang MB, Kositratana W and Gong Z (1998) Production of transgenic rice with rice ragged stunt virus synthetic resistance genes. Acta Horticulturae. 461:393-400 (times cited: 0). Wang MB
[DOCX File]ATTACHMENT 2 - Food and Agriculture Organization
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No DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred to the plant. As a result of the integration of the three expression cassettes, there is a 3 bp insertion at the 5’ junction region and a deletion of 4,385 bp of host DNA.
[DOC File]ATTACHMENT 2
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Agrobacterium mediated transformation involves incubation of the bacteria carrying the particular plasmid with plant cells for a few hours to days, during which time T-DNA transfer takes place. The cells are then washed and cultured in the presence of the selection agent, glufosinate ammonium, and transformed shoots are regenerated and ...
[DOC File]SLOVENSKÁ POĽNOHOSPODÁRSKA UNIVERZITA V NITRE
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Agrobacterium mediated transformation as the most frequently used method is written in more details. In addition, the work brings overview about the types of selectable marker and reporter genes. The second part of the work is devoted to the target transgenosis.
Transformation - Oregon State University
Wheat and other monocots have proved more difficult than dicots to transform and it is only recently that agrobacterium-mediated transformation has worked with wheat (Cheng et al., 1997). More commonly, wheat has been transformed using a biolistic delivery method.
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