Agrobacterium mediated transient expression
[PDF File]Sonication-assisted Agrobacterium-mediated transformation ...
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Key words Agrobacterium tumefaciens · Glycine max· Transient expression · SAAT · Transformation Introduction Agrobacterium provides one of the main vehicles for in-troducing foreign DNA into plants. A number of plant spe-cies (Wordragen and Dons, 1992; Fisk and Dandekar, 1993) and even yeast (Bundock et al. 1995) have been
[PDF File]Agrobacterium infiltration of Nicotiana benthamiana for ...
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Agrobacterium infiltration of Nicotiana benthamiana for transient protein ... Transient expression in the wild tobacco plant (Nicotiana benthamiana) is used to determine the subcellular location of a protein of interest when tagged with a reporter ... Agrobacterium-Mediated Infiltration of N. benthamiana This method was modified from Voinnet et ...
[PDF File]A Highly Efficient Agrobacterium-Mediated Method …
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A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species Youjun Zhang1 , 25*, Moxian Chen3, Beata Siemiatkowska2, Mitchell Rey Toleco2, Yue Jing , Vivien Strotmann4, Jianghua Zhang3, Yvonne Stahl4 and Alisdair R. Fernie1 ,2 * 1Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria
A simple and efficient Agrobacterium‐mediated transient ...
A simple and efficient Agrobacterium-mediated transient expression system to dissect molecular processes in Brassica rapa and Brassica napus Brian C. Mooney1 | Emmanuelle Graciet1,2 This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,
Agrobacterium-Mediated Transient Protein Expression in ...
Mar 12, 2008 · also amenable to Agrobacterium-mediated transient expression, a rapid, scalable and low-cost gene delivery system. When co-cultured with plant cell suspensions, recombinant Agrobacteria can efficiently deliver heterologous DNA to the nucleus of virtually every plant cell. Transient expression of …
[PDF File]Development of Agrobacterium-mediated transient expression ...
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factant for Agrobacterium-mediated transient expression in C. intermedia leaves and the GUS reporter gene expressed to the highest level at 5~7days after infiltration. To investigate the effect of surfactant concentration on transient expression efficiency of C. intermedia,we select Silwet L-77 as the suitable surfactant based on the above results.
AGROBEST: an efficient Agrobacterium-mediated transient ...
Agrobacterium -mediated DNA transfer is currently the most facile and versatile method to deliver gene constructs into the nucleus for gene function analysis in diverse plant species [1-3]. Although stable integration of physiologically active and regulated transgenes is the ultimate goal, transient gene expression via Agrobacterium-mediated
[PDF File]Agrobacterium-mediated transient expression via root ...
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Benchabane et al. 2009). For Agrobacterium-mediated transient expression, leaf infiltration is the most common and efficient strategy used for Agrobacterium-mediated transient expression (Bhaskar et al. 2009; Voinnet et al. 2003). However, the method has limited applications for research involving roots, since the effects are mainly Open Access
[DOC File]Interaction of MYB and bHLH transcription factors in ...
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High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions. Plant J. 41: 162-174. Pattanaik, S., Xie C.H. and Yuan L. 2008. The interaction domains of the plant Myc-like BHLH transcription factors can regulate the transactivation strength. Planta 227: 707-715.
[DOCX File]TECHNICAL ADVANCE
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Agrobacterium-mediated transient expression system. Plant Physiol. 126, 930-938. Lai, H., and Chen, Q. (2012) Bioprocessing of plant-derived virus-like particles of norwalk virus capsid protein under current good manufacture practice regulations. Plant Cell Reports . 31, 573-584.
[DOC File]Cell
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Agrobacterium-Mediated Transient Expression, Protein extraction and Western blot analysis. Agrobacterium tumefaciens strain EHA105 and GV3101 (pmp90) were used in these experiments. The procedures for agrobacterium infiltration, protein extraction and western blot s were carried out as previously described (Liu et al., 2010; Zhu et al., 2005).
[DOCX File]Home - Chlamydomonas Resource Center
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The mechanism by which transient expression of the Cas9/intron-sgRNA construct succeeds in Chlamydomonas (e.g., more stoichiometric or better balanced production of Cas9 and sgRNA from the single hybrid gene) will be challenging to establish given the apparently low percentage of the cell population that is successfully modified.
[DOC File]VIGS as a post transcriptional gene silencing mechanism in ...
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Since plants infected by many viruses induce RNA mediated defense which targets viral RNAs and any transgene RNA products inserted into it [6]. As a gene silencing method VIGS has several advantageous such as fast, transient suppression of gene expression and it involves cloning of short sequence fragments of targeted gene to be silenced.
[DOC File]Digital CSIC
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The introduction of DNA fragments through agrobacterium-mediated transfer of T-DNA was found to be a powerful research tool that allows the transient expression of any gene in a plant [9-12] after Agrobacterium tumefaciens had become of routine use to transform plants stably and constitutively.
[DOC File]Supplemental material
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May 07, 2004 · Silencing on the spot: induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol. 126: 930-938. Llave, C., Kasschau, K.D., and Carrington, J.C. 2000. Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway. Proc. Natl.
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In this study, we investigated the potential for BTV VLPs to be produced in plants via Agrobacterium-mediated transient expression using four different plant expression vectors, as an inexpensive ...
CURRICULUM VITAE - NIGEB
1. Agrobacterium-mediated transformation of EPSPS genes into Brassica napus. 2. Isolation and characterization of ribosomal protein L3 homologous genes, responsible for Fusarium Head Blight resistance in wheat. 3. Cloning and over-expression of P5CS genes in transgenic plants in order to increase osmotic resistance. 8. INTERNATONAL GRANTS: 1.
[DOC File]Research and Development
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Transient expression was observed in the scutellum of inoculated embryo, a non regenerable tissue in oat. Thus T-DNA transfer occurred at reasonably high frequency with all AGL1 strains tested, but at very low frequency with LBA4404 harbouring the new vectors or with LBA4404/pTOK233. ... Perret S. 1999 Agrobacterium-mediated transformation of ...
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