Ana pattern nuclear speckled
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The immunology panel showed positivity for antinuclear antibodies at titer 1/320 (speckled pattern), positivity for antibody anti-SSA and also anti-dsDNA, with absence of anti-SSB, anti-RNP. The clinical picture was interpreted as NLE-related pneumonitis and the patient received intravenous methylprednisolone pulses (30 mg/kg/day) on 3 ...
[DOC File]Semi-quantitative latex agglutination test detect acute ...
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ANA: There are different nuclear fluorescent staining patterns that can coexist in the same serum. The pattern may be modified with the dilution of the serum. There are 4 main antinuclear patterns. Homogeneous: Homogeneous and uniform fluorescence throughout the nucleus of interphase cells. Strong fluorescence of mitotic cells. Peripheral:
[DOCX File]RDS | Rheumatologic Dermatology Society
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Physical examination revealed scaly, erythematous plaques on the interdigital and dorsal surfaces of the fingers. There were no lesions on the head or neck or other locations. The patient’s ANA was positive at a titer of 1:40, in a speckled pattern, and SS-A antibody was positive at 1.4 (nl < 1.0).
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Speckled pattern. Nucleolar pattern . Homogeneous or diffuse nuclear staining usually reflects antibodies to chromatin, histones and, occasionally, double-stranded DNA. Rim or peripheral staining patterns are most commonly indicative of antibodies to double-stranded DNA. Speckled pattern refers to the presence of uniform or variable-sized speckles.
[DOCX File]Zeus Scientific, Inc
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The ZEUS ANA HEp-2 Test System is designed to detect the presence of circulating ANA in human sera. ... Dripper-tipped bottle containing human ANA-positive control serum producing 4+ speckled nuclear staining with the substrate cells. ... A positive reaction is the presence of any pattern of nuclear apple-green staining observed at a 1:40 ...
[DOC File]Diabetes - Stanford University
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ANA pattern Nuclear antigen Clinical associations Homogeneous/diffuse Histone/DNA SLE, drug effect Speckled Various MCTD, SLE, Sjögren’s, poly-/dermatomyositis, various autoimmune diseases, infection, neoplasia Nucleolar RNA-associated antigens Scleroderma Peripheral (rim) DNA SLE Centromere Centromere Limited scleroderma ...
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Nuclear fine speckled. Dermatomyositis (99% specificity and 4-18% sensitivity). Less common in juvenile dermatomyositis than the adult form. Of those positive 95% have dermatomyositis and 3% polymyositis. PCNA Pleomorphic speckled nucleoplasmic staining, with variability in size and brightness of the speckles. 3-6% SLE, very specific.
[DOC File]АНТИНУКЛЕАРНИ АНТИТЕЛА -КЛИНИЧНО ЗНАЧЕНИЕ
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ANA immunofluorescent patterns. IIFA PATTERN ANA against Disease Homogenous nuclear. dsDNA, ssDNA, histones SLE, low titres in other CTD Nucleolar (homogenous, clumpy or speckled) PM/Scl, Ku, others High titres in SScl, SS Sp. e. ck. led (large, coarse or fine) SS-A, SS-B, Sm, RNP, Scl 70 SLE, MCTD, SScl, SS Centromere CENPs CREST, SScl
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lupus/ANA screen. rheumatic fever/ ASO screen. 10/23/07[1] ... Nuclear maturation lags behind cytoplasmic maturation. ... RBCs lyse on contact with the reagent pad, causing a positive reaction (speckled pattern may result if low-grade) Mechanism - free Hb filtered into urine as a result of hemoglobinemia (usually detectable as visibly red ...
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Anti-Nuclear Factor. Anti-nuclear factor screening is routinely carried out on patients with various connective tissue diseases, particularly Systemic Lupus Erythematosus (SLE). The inclusion of a HEp2 cell in ANA testing enables the laboratory to recognise at least 5 nuclear fluorescence patterns.
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