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[DOC File]Bacterial Transformation - AP Environmental Science
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Bacterial Transformation. I. Objectives. After completing this activity, you should be able to: Describe the process of transformation and outline the steps involved in this technique. Explain the purpose of the use of calcium chloride and the heat shock step. Explain why antibiotic-resistance genes are …
steps for bacterial transformation

[DOC File]Name:
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BACTERIAL TRANSFORMATION. Nomenclature. 1. Donor cell (DNA) 2. Recipient Cell. 3. Transformant (recipient with donor DNA) Features. 1.low frequency event. 2.requires homologous recombination. 3. one of the best ways to introduce altered DNA into a cell. DNA uptake can be greatly enhanced. (104 - 106 increase) If incubate cells under certain ...
heat shock dna transformation

[DOC File]BIOTECH Laboratory Activity:
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Heat Shock: The cell/DNA suspension is briefly incubated at 42°C and then quickly returned to 0°C. The rapid temperature change creates a thermal imbalance (gradient) on either side of the . E. coli . membrane, which is thought to create a draft that sweeps plasmids into the cell. Recovery
bacterial transformation methods

[DOCX File]LAB 16 - Bacterial Transformation
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Bacterial . Transformation* How can we use genetic engineering techniques to . ... "Heat shocking" the cells . 3. Incubating the cells in nutrient broth for a short time before ... "heat shock" the cells in the tubes. It is critical that the cells receive a sharp and distinct . shock! Make sure the tubes are closed tightly! Place the tubes into ...
heat shock bacteria

[DOC File]Bacterial Transformation - Metabolism
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TEACHER GUIDE: Bacterial Transformation with Mystery DNA. This teacher guide is provided to give sample answers to questions. Most of the questions are open-ended, so students may have correct answers that aren't included in this guide. ... It's called heat shock. Back to the ice which acts to close the holes in the cell and keep the DNA inside ...
purpose heat shock bacterial transformation

Bacterial Transformation - Gene Transfer, Heat Shock, Vs Transduct…
Heat shock: First, the bacteria is cooled to a low temperature for several minutes, usually with an ice bath. The tube is then quickly moved into 42°C warm water. This sudden change in temperature causes the pores of the bacteria to open up to larger sizes, allowing the plasmid to enter.
heat shock method for transformation

[DOC File]AP Biology - local-brookings.k12.sd.us
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Bacterial Transformation and Protein Purification. Group 4. Natalie Beale Gregory A. Pate. Justin Rousseau. Dohee Won ... Heat shock the tubes. Place foam rack in 42°C water bath for 50 seconds. After this, place on ice for 2 minutes. Remove rack from ice and place on counter. Transfer 250 μl of LB broth to each tube and incubate at room ...
e.coli transformation heat shock

Bacterial transformation heat shock