Bacterial transformation lab answers
[DOC File]Central Bucks School District / Homepage
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Table 2. Results from Transformation Lab. LB/amp +pGLO. LB/amp/ara +pGLO LB/amp -pGLO LB -pGLO. Number of Colonies* Was the bacteria transformed? Did the bacteria glow? Analysis Questions ( Copy these questions and write the answers in your lab notebook. Did the pGLO plasmid in the +pGLO vial glow when you shined UV light on it? Why or why not?
[DOC File]BIOTECH Laboratory Activity:
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Figure 1: Transformation of pGLO plasmid DNA (Biorad Laboratories, Inc.) In this laboratory, you will first make bacterial cells competent, and then introduce plasmid DNA by transformation. One plasmid, pGLO, contains the Green Fluorescent Protein (GFP) gene from Aequorea victoria, a bioluminescent jellyfish.
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During this lab, we will 'poke holes' in the bacteria using chemicals, allowing the DNA to flow into the bacteria- this is called BACTERIAL TRANSFORMATION. Why would we want to put DNA into bacteria? We can use bacteria as little 'factories' to make more DNA, as they replicate, or to make protein, by transforming them with genes for proteins we ...
[DOC File]AP Biology Molecular Biology Lab Worksheet
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AP Biology Molecular Biology Lab Bacterial Transformation Worksheet 4-17-13 *Below is the link to the new Free-Response Booklet which shows how questions and space for answers will be distributed throughout the exam booklet. This will help you to prepare for what the exam will look like.
[DOC File]AP Biology
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In your lab notebook, record data, answers to questions, and any questions that arise . during this part of the activity. Step 1 Form lab teams, as instructed by your teacher. Familiarize yourself with sterile . technique, materials and lab equipment, and safety procedures for handling bacteria and decontaminating the work area.
[DOC File]Welcome to Science with Ms. Wood!
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Glowing Bacteria: Transformation of the bacteriaum Escherichia coli (E. Coli) with a Jelly Fish Gene ***The following lab uses a procedure adapted from BioRad*** Overview Plasmids containing specific fragments of foreign DNA will be used to transform Escheruchia coli cells, passing on antibiotic (ampicillin resistance) and foreign gene ...
[DOCX File]LAB 16 - Bacterial Transformation
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Bacterial . transformation . involves transfer of genetic information into a cell by direct uptake of the DNA. During gene transfer, the uptake and expression of foreign DNA by a recipient bacterium can result in conferring a particular trait to a recipient lacking that trait. ... LAB 16 - Bacterial Transformation
[DOC File]MS BRADY'S CLASSROOM WEBSITE - Ms. Brady's Website
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Glowing Bacteria: Transformation of the Bacteriaum Escherichia coli (E. coli) with a Jelly Fish Gene AP Lab Invertigation 8 – Biotechnology: Bacterial Transformation. How can we use genetic engineering techniques to manipulate heritable information? ***The following lab uses a procedure adapted from BioRad*** Mice that glow fluorescent green.
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Bacterial Transformation with “glowing” DNA (GFP on a plasmid) This teacher guide is provided to give sample answers to questions. Most of the questions are open-ended, so students may have correct answers that aren't included in this guide. Finally, although the experiment is set up to yield one correct answer, there are variations in data ...
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