Bl21 de3 genotype

    • [DOCX File]6-Deoxyerythronolide B (6dEB), the macrocyclic core of the ...

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      BL21 (DE3) harboring the plasmid pKW19211 or pKW19212 was grown overnight in 20 mL of LB (lysogeny broth) with 50 µg/mL kanamycin at 37 C. Three liters of fresh LB with 50 µg/mL kanamycin was inoculated with 5 mL of the overnight culture and incubated …

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    • [DOC File]Kashan University of Medical Sciences

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      Rohani M, Moniri R, et al. Beijing genotype and other predominant Mycobacterium tuberculosis spoligotypes observed in Mashhad city, Iran. Indian Journal of Medical Microbiology Volume 27, Issue 4, 1 October 2009, Pages 306-310.

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    • [DOC File]Supplemental Materials - Genes & Development

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      Nov 16, 2006 · Strain Genotype and relevant features Reference DH-E113 BL21(DE3) Novagen DH-E121 pET28a in BL21(DE3) Novagen DH-E122 pET29b in BL21(DE3) Novagen DH-E123 pCON1 in JM109 (Higgins 2006) DH-E182. XL1-Blue {F’ proAB lacIq ∆(lacZ)M15 Tn10} recA1 endA1 gyrA96 thi-1 hsdR17 supE relA1 lac Stratagene. DH-E278 JM109(DE3) pLysS Novagen DH-E375

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    • [DOCX File]UvA

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      BL21(DE3) F– ompT hsdSB(rB– mB–) gal dcm (DE3) Novagen. BW25113. lacIq rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 (Datsenko and Wanner, 2000) BW25113Δ6LDT. lacIq rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78. ΔycbB ΔerfK ΔycfS ΔybiS ΔynhG ΔyafK (Kuru et …

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    • [DOC File]The Lancet

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      Recombinant preS protein comprising preS1 + preS2 from HBV genotype A2, (Fig. 2) (GenBank: AAT28735) and a C-terminal hexahistidine tag was expressed in E. coli BL21 (DE3) (Agilent Technologies, USA) as described [18].

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    • [DOC File]Supporting Table 4 - PNAS

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      May 18, 2010 · Strain, plasmid, or primer Genotype, phenotype, or sequence of primer (5’ to 3’) Reference ... 567 ΔdapA1341::[erm pir] W. Metcalf BL21(DE3) F- ompT hsdSB(rB-mB-) gal dcm (DE3) Invitrogen BL21-DE3(p42-0119) BL21(DE3) containing p42-0119 This study WM3064 (pKm-0117) WM3064 containing pKM-0117 This study WM3064 (pJK-hcp-nhaA) WM3064 ...

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    • [DOC File]Welcome to CaltechAUTHORS - CaltechAUTHORS

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      Supplemental Information. Table A: Strains and plasmids disclosed in this study. Strains and plasmids Genotype E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5] E. coli 1993 E. coli BW25113, ldhA-fnr::FRT, adhE::FRT, frd::FRT, pflB::FRT, F'

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    • [DOC File]PATh :.: Process and Product Applied Thermodynamics ...

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      Table A.1 E. coli strains used in this work.. E. coli strain Genotype Selection marker Manufacturer DH5α1 [deoR endA1 gyrA96 hsdR17 Δ(lac)U169 recA1 relA1 supE44 thi-1 (Φ 80 lacZΔM15)] - Novagen™ AD4942 [Δ(araABC-leu)7697 ΔlacX74 ΔmalF3 ΔphoAPvuII phoR trxB ::Kanr F'[lacIqlacZΔM15proAB+] Kan Novagen™ BL21(DE3)3 [F– ompT hsdSB(rB–, mB–) gal dcm (DE3)] - Life Technologies ...

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    • [DOCX File]Addgene

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      All strains are derivatives of BL21(DE3) (genotype F– ompT gal dcm lon hsdS B (r B –m B –) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+] K-12. (λS)) The strains lack the coding sequence of one or more abundant outer membrane proteins (OmpA, OmpC, OmpF, LamB)

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    • [DOC File]Table 1 - Genes & Development

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      Oct 15, 2009 · Strains/plasmids Relevant genotype Source/reference Strains E. coli DH5 fhuA2 (argF-lacZ)U169 phoA glnV44 Φ80 (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 Invitrogen BL21(DE3)pLysS dcm ompT hsdS(rB-mB-) gal (DE3), pLysS, CmR Invitrogen CAG22239 MG1655 lacX74 Lab collection CAG57101 MG1655 lacX74, rpoH::aadA, PBAD_groESL::cat CmR, SpR (Koo ...

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