Cell signaling technologies

• [DOC File]Western Blotting Conditions - University of Virginia

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  -Cell Signaling Technology (#3161)-run 20 ug total protein.-use 1:1000 dilution of primary antibody (Cell Signaling #3161) in . 5% BSA in 1X TBS-T overnight-use anti-rabbit secondary antibody (1:3000).-block blot in milk. Phospho-ERK1/2 (Cell Signaling 9101S)-run 10 ug total protein-use 1:1000 dilution of primary antibody

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• [DOCX File]www.cell.com

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  Antibody Name: Source Species. Company. Catalog Number. Dilution IHC. Dilution WB. 4E-BP1. Rabbit. Cell Signaling. 9644 1:1000. phospho-4E-BP1 (Thr37/46) Rabbit. Cell ...

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• [DOC File]Cell Biology Lecture Notes - Seton Hall University

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  Cell Signaling. Cell-cell communication in animal cells Via secreted molecules paracrine signaling endocrine signaling synaptic signaling Via plasma-membrane-bound-molecules Cell adhesion, cell junction and extracellular matrix Receptors and hydrophobicity of signaling molecules Cell surface receptors and hydrophilic signaling molecules Intracellular receptors and hydrophophobic molecules ...

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• www.cell.com

  Antibody. Clone. Source. Identifier. dsDNA. 35I9 DNA. Abcam. ab27156. Vimentin. D21H3. Cell Signaling Technology. Custom Purified. Histone H3. D1H2. Cell Signaling ...

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• [DOC File]Supplementary Figure 1

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  2006-05-16 · The primary antibodies used for immunohistochemistry were anti–phospho (Ser10) histone H3 (used at a 1:200 dilution, #9701 Cell Signaling Technology); anti–cleaved (Asp175) caspase-3 (used at a 1:200 dilution, #9661 Cell Signaling Technology), anti-EGFR (K1492, Dako Pharmdx Kit), anti-EGFRL858R (used at a 1:300 dilution, see below for a description of this antibody), anti-prosurfactant ...

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• [DOCX File]Home | Molecular Cancer Therapeutics

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  Additional primary antibodies used were anti-phospho-Histone H3 (#9701, Cell Signaling Technologies; 0.13 mg/mL), and control rabbit IgG. Toxicity and Toxicok. i. n. e. tic Assessments of . Conventional and . Site-Specific HER2 ADC in Rats and Non-Human Primates. All animal studies were approved by the Pfizer Institutional Animal Care and Use Committee according to established guidelines. Rats ...

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• [DOC File]Material and methods

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  2009-08-27 · Grids with sections were blocked with 1% BSA in 1X PBS for one hour at room temperature and incubated with rabbit anti-LC3 (1:25, Cell Signaling Technologies) and sheep anti-SOD-1 (1:75, Calbiochem), 0,1% BSA in 1X PBS overnight at 4oC. Grids were washed and incubated with goat anti-rabbit IgG conjugated to 10 nm gold colloid (1:20, Ted Pella) and donkey anti-sheep IgG conjugated to …

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• [DOCX File]Table S - The Lancet | The best science for better lives

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  When specified, primary hepatocytes isolated from wild-type mice were pretreated for 1 hour with 50 μM PI3K inhibitor (LY294002 - Cell Signaling Technologies, Boston, United States) or in other experimental settings with 50 nM mTOR inhibitor (Rapamycin - Cell Signaling Technologies, Boston, United States), and then exposed to 0.33 μM insulin (Insuman Rapid, Sanofi Aventis) for 6 hours.

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• [DOCX File]HUSCAP

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  Cell Signaling Technologies. Cat#2932. Rabbit anti-β-actin. Cell Signaling Technologies. Cat#4970. Horse anti-mouse IgG HRP-linked. Cell Signaling Technologies. Cat#7076. Goat anti-rabbit IgG HRP-linked. Cell Signaling Technologies. Cat#7074. Goat anti-rabbit IgG Alexa Fluor® 488-linked. Abcam. Cat#ab150077. Author: Frontiers Media SA Created Date: 05/29/2019 18:04:00 Last modified by ...

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• [DOCX File]Genes & Development

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  2021-02-17 · Incubation with primary antibody anti-HA C29F4 (Cell signaling technologies, 1:50) was done overnight at 4°C in a humid chamber, followed by secondary antibody incubation for 2 hours at room temperature.Quantitative polymerase chain reaction (qPCR)

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