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[DOC File]ARCHIVED: 2006 Science and Technology/Engineering ...
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3.1 Describe the basic structure (double helix, sugar/phosphate backbone, linked by complementary nucleotide pairs) of DNA, and describe its function in genetic inheritance. 3. Genetics (cont.) 3.2 Describe the basic process of DNA replication and how it relates …
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[DOCX File]Baylor’s
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Do you like to build models using Legos and K’NEX? If you do, this course is for you and other young engineers. Learn about and build basic simple machines, motorized machines, and robots as well as photosynthesis, chromosomes and DNA models--- all in a fun-filled, hands-on way. Plans are made to visit the Mayborn Museum to see the Da Vinci ...
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[DOCX File]Lippincott Williams & Wilkins
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Microbial DNA was extracted from 1.4 ml stabilized stool (stool specimen homogenized in stool DNA stabilizing buffer) using the PSP® Spin Stool DNA Plus Kit (STRATEC Molecular, Birkenfeld, Germany) according to the manufacturer’s protocol 2 (“Isolation of total DNA from 1.4 ml stabilized stool homogenate with enrichment of bacterial DNA”).
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[DOC File]PCR
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c. Extend using DNA polymerase (Taq @ 72 °C) Time depends on length and concentration of template. Repeat cycle up to ~ 30 times. Limited by stability of polymerase, concentration of primers and dNTPs C. Analysis. 1. Gel electrophoresis (what we do) a. Gel purification--cut out bands and reisolate product using Qiagen kit. b.
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[DOCX File]DNA Cheek Cells TN - Serendip Studio's One World
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During DNA replication, the two strands of the original DNA double helix are separated and each old strand is used as a template to form a new matching DNA strand. The enzyme DNA polymerase adds nucleotides one-at-a-time, using the base-pairing rules to match each nucleotide in the old DNA strand with a complementary nucleotide in the new DNA ...
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[DOC File]Protocol for Real-Time RT-PCR - Harvard University
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25 bp DNA ladder (Invitrogen). ABI Prism 7000 Sequence Detection System (Applied Biosystems). ABI Prism 7000 SDS software (Applied Biosystems). 3% ReadyAgarose Precast Gel (Bio-Rad). Agarose gel electrophoresis apparatus (Bio-Rad). Detailed procedure. Reverse Transcription
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[DOCX File]3dq2s3got0l1esv542z3e0z1-wpengine.netdna-ssl.com
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The Panel Lantern is available as a kit which includes the LED head unit, a 24”x24” collapsible Chimera Panel Frame, Lantern Fabric, dimmer, 12 ft. head power cable and power supply. The kit fits into a compact 16x28x3” (406x711x76 mm) ballistic bag and weighs less than 6 lbs (2.7kg), which equals ultimate portability.
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[DOCX File]Blood Type TN - Serendip Studio's One World
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Non-porous testing surfaces suitable for mixing two samples of blood with antibody solution, e.g. blood-typing trays, microscope slides or white or transparent plastic lids (can be washed and reused in multiple classes) (If you use the recommendations for implementation shown below, each student group will need a testing surface large enough for 14 tests (two tests for each subject) or several ...
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