Fmoc deprotection protocol
Introduction -ouvertes.fr
The base-labile Fmoc temporary group represents the second dimension, and the third one is provided by the allyl group, which can be removed with Pd(PPh3)4 in anhydrous DCM and Bu3SnH or Ph3SiH can be added as allyl-group scavengers., But since nucleophilic species act as allyl scavengers, allyl deprotection should be performed before the ...
[DOC File]Supporting information
https://info.5y1.org/fmoc-deprotection-protocol_1_70e0c4.html
Peptide 1 was synthesized on an Applied Biosystems 433A peptide synthesizer using the FastMoc protocol on Fmoc-Alg-O-ArgoGel on a 0.25 mmol scale. Each synthetic cycle consisted of N(-Fmoc removal by a 10 min treatment with 20% piperidine in NMP, a 6 min NMP wash, a 45 min coupling step with 1.0 mmol of preactivated Fmoc amino acid in the ...
[DOC File]Supplemental Data
https://info.5y1.org/fmoc-deprotection-protocol_1_141ab7.html
Aug 16, 2007 · Peptides were synthesized by standard Fmoc chemistry using an AbiMed ASP422 robot. Following peptide synthesis and side-chain deprotection, membranes were blocked overnight in 5% skim milk. Purified GST-PBD WT or GST-PBD AA fusion proteins were added at 1 µg/ml in TBS and incubated for 2 h at 4 °C.
[DOCX File]Lecture 1: Key Concepts in Stereoselective Synthesis
https://info.5y1.org/fmoc-deprotection-protocol_1_d9922c.html
Fmoc strategy allows for milder deprotection scheme and is considered truly orthogonal. No special equipment needed. ... Their protocol consists of column packed with activated MnO. 2 . to generate a transient diazo compound that is quenched in flow with a variety of carboxylic acids to yield the corresponding ester products. A wide range of ...
[DOC File]Supporting information
https://info.5y1.org/fmoc-deprotection-protocol_1_4fda06.html
Dec 30, 2015 · For Fmoc deprotection, beads were incubated with 20% 4-methyl piperidine solution in DMF for twice (5mim, 15min) and then thoroughly washed with DMF, MeOH and DMF three times each, respectively. For Alloc deprotection, the resulting beads were incubated with (Pd(PPh3)4) (0.2 eqiv) and (PhSiH3) (20eqiv) in DCM for twice (30min each time), and ...
[DOCX File]Adam G. Kreutzer, Patrick J. Salveson, Hyunjun Yang ...
https://info.5y1.org/fmoc-deprotection-protocol_1_dc832b.html
Standard practices for Fmoc-based solid-phase peptide synthesis in the Nowick laboratory (Version 1.7.2). Adam G. Kreutzer, Patrick J. Salveson, Hyunjun Yang, Gretchen Guaglianone. E-mail: jsnowick@uci.edu. Contents. 1. Contributions to this guide2
[DOCX File]Welcome to CaltechAUTHORS - CaltechAUTHORS
https://info.5y1.org/fmoc-deprotection-protocol_1_85f1e8.html
Deprotection of the Fmoc group required 20% (v/v) piperidine/NMP, followed by wash with NMP. ... The first amino acid was attached to the resin following the vendor’s protocol. The resin was transferred to the CEM Liberty 1 for the coupling of the remaining amino acids. Each amino acid coupling reaction incorporated 4 equiv of Fmoc-amino acid ...
Nearby & related entries:
To fulfill the demand for quickly locating and searching documents.
It is intelligent file search solution for home and business.