How to determine the concentration c1v1c2v2
[DOC File]Measuring Spore Concentration with Hemacytometer
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Measuring Spore Concentration with Hemacytometer. Mix spore solution well. Add 10 µl of spore solution to each side of the hemacytometer. Count number of spores in ...
[DOC File]Concentration and Dilution Lab
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Part B: Concentration and Dilution Lab. Objectives. To mix a solution and determine its concentration. To perform three dilutions with your prepared solution. Concentration Procedure. Obtain a sample of CuSO4 from the front bench. Using your scale weigh out 18g of CuSO4. Dissolve the 18g of CuSO4 in 100ml of distilled water.
[DOC File]James Madison University
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To determine your DNA concentration raise 10 to the number you get when solving for x (10x = sample DNA concentration). Note that your sample CT values must fall on the curve for it to be of use. CT values which are too large or small to fit on the curve cannot be used to extrapolate your DNA concentration.
[DOC File]Chemistry 1215 Experiment #12
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The molar absorptivity of methyl viologen at a wavelength of 600 nm is 13,000 L/(cm.mol).Use Beer’s law to determine the concentration of a methyl viologen solution that has an absorbance of 1.600 in a 1.00 cm cuvette. Appendix 1: Using a Spectronic 20 (From the Dartmouth University website)
[DOC File]BIOLOGY 4503 TECHNIQUES IN BIOCHEMISTRY LABORATORY
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Desired concentration (mg/mL) Absorbance at 595nm 0.025 0.1051 0.05 0.2285 0.1 0.2891 0.15 0.5856 0.2 0.7815 0.25 0.9709 0.3 1.079 0.35 1.184 0.4 1.34 blank 0.4658 As concentration increases, so does absorbance showing a direct relationship; which is confirmed by the Beer-Lambert law of …
[DOC File]Experiment 6
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Use your Beer's law plot to determine the concentration (mg/mL of Fe) of each of the four unknown Fe(Ph)32+ solutions. Keep in mind that the test unknown solutions were prepared via dilution of your stock solution. Thus, you will need to use the equation: C1V1 = C2V2 to get the original concentration of your unknown solution. b.
[DOC File]Solutions (Heath Chapter 16)
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Solutions (Heath Chapter 16) p.5 *T*1) Determine which of the following compounds are insoluble in water: Refer to ‘solubility’ sheet. a) sodium hydroxide -soluble b) ammonium acetate-soluble
[DOC File]AP BIO EQUATIONS AND FORMULAS REVIEW SHEET #1
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Graph these data to the right of the table. From your graph, label where the cells were hypotonic and the solution was hypertonic, and vice versa. Determine the apparent molar concentration (osmolarity) of the potato core cells. Osmolarity = 0.3M cell and beaker are isotonic
[DOC File]Measuring Spore Concentration with Hemacytometer
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67,000 So, you will add 30mL spore solution to 10mL sterile dH20 (40-30=10) to give a final concentration of 50,000 spores/mL in 40mL. You can use the Excel worksheet: C:\Scab data\Protocols\Current\Hemacytometer Worksheet.xls . to print out a data entry form or to calculate spore concentrations. Nicki Mundell Page 1 2/1/2007
[DOC File]Got Protein - MassBioEd
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2. Plot your data on the graph below to create a standard curve (BSA concentration vs. Absorbance at 540 nm). Reminder: label your x and y-axis and title your graph. Post-Lab Analysis: 1. Using your standard curve, determine the concentrations of your unknown solutions. Record the results in Table 1 Protein Concentration and Absorbance at 540 ...
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