How to dilute dmso liquid
[DOC File]Protein Expression and Purification - Hahn Lab
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Dissolve a small amount dye in 50µL DMSO (a few mg. Do not try to weigh it – static charge will likely cause you to lose much of the dye). Dilute in DMSO until the absorbance max at the long wavelength …
[DOC File]Isolation of Human PBMC from Whole Blood
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Count on the ViCell (or Hemocytometer with viable cell exclusion dye), and dilute the cells to 2x10^7 cells/ml (but not less than 1.5 ml minimum). Slowly add an equal volume of 2X freezing medium. …
[DOC File]SAMI Editor Redesign
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Fourth, dilute the sample compounds in either Tris-HCl buffer or DMSO and Tris-HCl buffer within the deep-well microplates. Fifth, transfer the sample compound to 96-well flat test microplates with single …
[DOCX File]www.tandfonline.com
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The plasma / recombinant FT 2-WT TTR exchange solution was then used to dilute drug stocks to their final concentrations of 30 M, 20 M, 10 M, 5 M, and 1 M, prepared separately for experiment 2, but in …
[DOC File]Methods Supplement - Nature
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They should then be cooled slowly to -80° (an isopropanol freezing chamber can accomplish this) before transferring to liquid nitrogen. The freezing media can vary in the concentration of FBS and the concentration of DMSO. Remember, DMSO …
[DOC File]Cryopreservation of Mouse Spermatozoa
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Cryoprotectant: DMSO. Mineral oil. Surgical equipment. Method. Remove cryotubes from liquid nitrogen and place them on the bench at room temperature until thawed (usually it requires about 10-15 min). When completely thawed, slowly add 0.8 ml of PBS in a drop wise fashion to dilute the DMSO.
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