How to make a 1 200 dilution
[DOC File]DILUTION PROBLEMS:
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How would you make a 4 mg/dL uric acid working standard from a 200 mg/dL stock uric acid standard? You have a serum with 7 g/dL of protein. What is the concentration in mg/dL of protein in the 6th tube of a 1:2 serial dilution of the serum?
[DOC File]Unit A Assignment Booklet A1 - LD Industries
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A 3.00-mol/L sodium phosphate solution, NaPO4(aq), is used to make 250 mL of 0.50-mol/L sodium phosphate solution. It is determined that 42 mL of the concentrated solution is needed for the dilution. Steps that may be used to make the solution are as follows:
[DOC File]Dilution of Solutions
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Step 1) Calculate the mass of sodium hydroxide required. Step 2) Add ~800 mL of COLD distilled water to a 1.0 liter volumetric flask. Step 3) Carefully, wearing safety goggles and rubber gloves, add 200 g of NaOH to volumetric flask. Step 4) Rinse the NaOH powder off the neck of the volumetric flask with distilled water from a squirt . bottle.
[DOC File]Isolation of Human PBMC from Whole Blood
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Make 1:200 dilution in CyPBS of Ir-intercalator. Add 20 uL of diluted Ir-intercalator solution to each sample, pipet to mix. Incubate on ice for 20 minutes. Wash x 3 in MilliQ water . Centrifuge cells at 2000rpm for 8 minutes at 4 (C. Aspirate. Acquire samples …
[DOC File]Iris Automated Urinalysis: iQ® 200 Analyzer SW 7.0 Template
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Urine Dilution Code labels [800-3211] Room temperature 1 set; 1 each/test as needed. Use as needed. 16x100mm glass tubes [660-0036] or polystyrene plastic tubes Room temperature Box of 200; 1 each/test. Use daily. Sample Patient Preparation: Give patient instructions to collect a “clean catch” urine specimen in a clean and/or sterile container.
[DOC File]MERCY MEDICAL CENTER - pSMILE
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1 square - multiply by 200 . 5 small squares - multiply by 1000. For very cloudy or bloody fluids requiring a final dilution of 1/200. Dilute specimen 1:100 with saline or Isoton (0.1 ml of specimen to 9.9 diluent). From the above dilution, make an additional dilution 1:2 using the appropriate diluting reagent (0.2 ml to 0.2 diluting fluid).
[DOC File]Title: Dilution Experiment
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P-200, P-20 Pipetman Dilution of a DNA Stock. Label 3 microcentrifuge tubes as dilution tubes number 1, 2 and 3. Pipet 90 µl of TE buffer solution into each microcentrifuge tube. (Use the P-200 pipetman) Pipet 10 µl of your DNA stock solution into each microcentrifuge tube. (Use the P-20 pipetman)
[DOC File]Lab Math - University of Washington
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Serial dilutions can be used to rapidly explore a wide range of concentrations. To make serial 2-fold dilutions, mix 1 mL of material with 1 mL of diluent. Take 1 mL of this 2-fold dilution and add 1 mL of diluent to make a 4-fold dilution. Take 1 mL of the fourfold dilution and add 1 mL of diluent to make an 8-fold dilution. And so on. Example:
[DOC File]Isolation of Human PBMC from Whole Blood
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Make 1:200 dilution in PBS of Ir-intercalator. Add 20 uL of diluted Ir-intercalator solution to each sample, pipet to mix. Incubate on ice for 20 minutes. Wash x 3 in MilliQ water .Centrifuge cells at 2000 rpm for 10 minutes at 4 (C. Aspirate. Bring up the samples in 1 …
[DOC File]DILUTIONS AND STANDARDS
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This means a 1/11 dilution was made since: volume of serum/total volume of solution = 1 mL serum/[1 mL serum +10 mL H20] = 1:11. 4. "Serial dilution" - This term is frequently used and refers to a "multiple" dilution problem. In other words, an initial dilution is made and then this dilution is used to make a second dilution, and so on.
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