Invitrogen dh5 alpha competent cells

    • [PDF File]Subcloning Efficiency DH5 Competent Cells

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      DH5 α™ competent cells support replication of M13mp vectors but do not support plaque formation. Plating a lawn of E. coli containing the F episome (e.g. DH5α-FT™, DH5αF'™, DH5αF'IQ™) will allow plaque formation. Components Supplied Amount DH5α™ Competent Cells 4 × 500 µl pUC19 Control DNA (100 pg/µl) 20 µl Genotype F- φ80lacZ∆M15 ∆(lacZYA-argF)U169 recA1 endA1 hsdR17(r ...

      competent cells protocol


    • [PDF File]MAX Efficiency DH5 Competent Cells

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      MAX Efficiency® DH5α™ Competent Cells Cat. No. 18258-012 Size: 1 ml Store at -70°C. Do not store in liquid nitrogen. Description: MAX Efficiency® DH5α™ Competent Cells have been prepared by a patented modification of the procedure of Hanahan (1). These cells are suitable for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The ...

      dh5a competent cells protocol


    • [PDF File]Subcloning Efficiency DH5 Competent Cells

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      Subcloning Efficiency ™ DH5α Competent Cells Cat. No. 18265-017 Size: 40 reactions Store at -80°C Description ... For technical support, email tech_support@invitrogen.com. For country-specific contact information, visit www.invitrogen.com. Page 2 General Guidelines Follow these guidelines when using Subcloning Efficiency ™ DH5α™ competent E. coli. • Handle competent cells gently as ...

      dh5 alpha transformation protocol


    • [PDF File]MAX Efficiency DH5 F'IQ Competent Cells

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      MAX Efficiency® DH5αF'IQ™ Competent Cells Cat. No. 18288-019 Size: 1 ml Store at -70°C Do not store in liquid nitrogen Description ... For technical support, email tech_support@invitrogen.com. For country-specific contact information, visit www.invitrogen.com. Page 2 Transformation Procedure A stock M13mp19 RF solution (0.01 µg/ml) is provided as a control to determine the transformation ...

      dh5a transformation protocol


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