Mobile phase usage calculator
[PDF File] Mobile Phase Preparation Guide - Waters Corporation
http://5y1.org/file/23545/mobile-phase-preparation-guide-waters-corporation.pdf
Preparation Procedure 3*. Add the indicated amounts of mobile phase buffers to 950 mL of water. Mix TEAA buffer solution thoroughly, measure pH, and adjust pH up ((CH3CH2)3N) or down (CH3COOH) to desired value. Add water to final volume of 1 L. Use this 100 mM TEAA buffer for mobile phase preparation described in step (4).
[PDF File] HPLC Method Development: Standard Practices and New …
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What are Some Standard Method Development Practices? 1. Follow preferred method development scheme and do “hands-on” method development – based on selectivity-changing parameters— e g pH column or mobile phase typese.g., pH, column, or mobile phase types 2. Use method development software – run a few predictive runs and get prediction ...
[PDF File] ACQUITY UPLC BEH Columns Care & Use Manual
http://5y1.org/file/23545/acquity-uplc-beh-columns-care-use-manual.pdf
To avoid precipitating mobile-phase buffers on your column or in your system, flush the column with five column volumes of a water/ organic solvent mixture, using the same or lower solvent content as in the desired buffered mobile phase.
[PDF File] Allowable Adjustments to United States Pharmacopeia (USP) …
http://5y1.org/file/23545/allowable-adjustments-to-united-states-pharmacopeia-usp.pdf
As of August 22, 2012 Ratio of components in mobile phase: ± 30 % (relative) The amount(s) of the minor component(s) (e.g. ≤ 50%) can be adjusted by ± 30 % relative. However a change in any component cannot exceed ± 10 % absolute. 60:40 Acetonitrile/Water could be adjusted to ± 12 % water (= 30 % of 40), but this exceeds the ± 10 % maximum absolute change. Therefore the …
[PDF File] The LC Handbook - Agilent
http://5y1.org/file/23545/the-lc-handbook-agilent.pdf
the sample component resides in a stationary phase relative to the time it resides in the mobile phase. It is calculated from the retention time divided by the time for an unretained peak (t 0). Selectivity or separation factor ...
[PDF File] COLUMN USER GUIDE for Agilent Normal-Phase and HILIC …
http://5y1.org/file/23545/column-user-guide-for-agilent-normal-phase-and-hilic.pdf
Column Usage Tips • Modern columns are robust and are designed to operate for long periods under normal chromatographic conditions. You can maximize ... Normal-phase columns can typically be stored in their own mobile phase for long periods. Before storing the column, the end-fittings should be tightly
[PDF File] Reductions in Cost and Time by Modernizing a USP …
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Abstract Modernization of the USP assay method for naproxen sodium tablets was performed to demonstrate the potential cost and time savings. Modernization of the method can drastically reduce both analysis time and mobile phase usage. To highlight this, a lifetime study of the UPLC column was performed and the results showed no change in performance over 10,000 …
[PDF File] CHROMATOGRAPHY - US Pharmacopeia (USP)
http://5y1.org/file/23545/chromatography-us-pharmacopeia-usp.pdf
hromatography. LC is a separation technique based on a solid stationary phase and a liqui. mobile phase.Separations are achieved by partition, adsorption, or ion-exchange processes, depending on the type of station. ry phase used. The most commonly used stationary phases are …
[PDF File] Chemistry 4631
http://5y1.org/file/23545/chemistry-4631.pdf
of column So selectivity must be achieved through changing the mobile phase. Mobile phase - solvent strength Variation in retention is generally achieved by altering the mobile phase. The interaction of a solvent molecule with an analyte depends on several forces: dispersion, dipole, hydrogen bonding and dielectric interactions (electrical ...
[PDF File] An Introduction to Gel Permeation Chromatography and Size …
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Vastly improve resolution and data quality in aqueous GPC. Gel Permeation Chromatography and Size Exclusion ChromatographyPRIMERStart hereThis guide provides some background to the most common techniques and applications of gel permeation chromatography, also known as size exclusion chromatography (GPC/SEC), for anyone not f.
[PDF File] Choosing Right Column for Reverse Phase HPLC Separations
http://5y1.org/file/23545/choosing-right-column-for-reverse-phase-hplc-separations.pdf
Before choosing the mobile phase for separating protein mixtures, consider whether proteins in the sample may exhibit non-ideal behavior. Most proteins are well behaved when separated on GF-250 and GF-450 (Figure 6).
[PDF File] A Practical Guide to High Performance Liquid Chromatography
http://5y1.org/file/23545/a-practical-guide-to-high-performance-liquid-chromatography.pdf
High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a stationary phase (chromatography column).
[PDF File] HILIC Method Development in a Few Simple Steps
http://5y1.org/file/23545/hilic-method-development-in-a-few-simple-steps.pdf
The mobile phase buffer pH can also affect the stationary phase charge state; this, for example is the case for silica phases, where the degree of silanol ionisation is dependent on the pH.
[PDF File] HPLC Separation Fundamentals - 121107 - Agilent
http://5y1.org/file/23545/hplc-separation-fundamentals-121107-agilent.pdf
Separation Fundamentals Agilent Restricted December 11, 2007. Major Separation Modes of HPLC A Review. There are four major separation modes that are used to separate most compounds: ¾Reversed-phase chromatography (most popular) ¾Normal-phase and adsorption chromatography ¾Ion exchange chromatography ¾Size exclusion chromatography.
[PDF File] Determination of Sugars in Molasses by HPLC Following Solid …
http://5y1.org/file/23545/determination-of-sugars-in-molasses-by-hplc-following-solid.pdf
An IS is preferred over the external standard (ES) because the IS can eliminate the problems concerning the injection of exact volumes into the liquid chromatography, variations in mobile phase composition, and potential changes in the aging of the column.
[PDF File] Lecture 4: Gas Chromatography Intro and Mobile Phase
http://5y1.org/file/23545/lecture-4-gas-chromatography-intro-and-mobile-phase.pdf
Lecture 4: Gas Chromatography Intro and Mobile Phase This method depends upon the solubility and boiling points of organic liquids in order to separate them from a mixture. It is both a qualitative (identity) and quantitative (how much of each) tool.
[PDF File] Tips and Tricks for HPLC and UHPLC - Thermo Fisher Scientific
http://5y1.org/file/23545/tips-and-tricks-for-hplc-and-uhplc-thermo-fisher-scientific.pdf
Common Problems: Mobile Phase. Solvent compatibility. Try to use pre-mixed solvents. Add 5-10% of organic eluent to the aqueous eluent. Add 5-10% aqueous eluent to the organic eluent. Avoids local crystallization in the pump (with buffers) Eluents with salt buffers. Change eluents with salt buffers regularly. Filtrate buffers.
[PDF File] A universal tool for method transfer from HPLC to UHPLC
http://5y1.org/file/23545/a-universal-tool-for-method-transfer-from-hplc-to-uhplc.pdf
For fast gradient methods, the gradient delay volume (GDV) plays a crucial role. The HPLC Method Transfer Calculator follows the gradient volume principle introduced by L. Snyder.2 The gradient volume is defined as the mobile phase volume that flows through the column in a defined gradient time or tG.
[PDF File] Waters UPLC, UHPLC, and HPLC Column Selection and Mobile …
http://5y1.org/file/23545/waters-uplc-uhplc-and-hplc-column-selection-and-mobile.pdf
Waters UPLC, UHPLC, and HPLC Column Selection and Mobile-Phase Guide [ waters.com ] Mobile-Phase Chemical pKa Buffer Range Formula Volume or Mass Required for 10 mM Mobile-Phase Concentration (per 1 L) pH Adjustment Acid/Base MS Compatible?
[PDF File] Preparation and utilization of monolithic column as HPLC …
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Preparation and utilization of monolithic column as HPLC stationary phase for alkyl benzene separation with low mobile phase usage Stevin Carolius Angga, Dias Septiana, Suci Amalia, Warsito ...
[PDF File] The Cleaning and Regeneration of Reversed-Phase HPLC …
http://5y1.org/file/23545/the-cleaning-and-regeneration-of-reversed-phase-hplc.pdf
`Reversed-phase chromatography is by far the most widely used technique in high performance liquid chromatography (HPLC).1 It is popular because it is applicable to most non-polar analytes and to many ionizable and ionic compounds. Most of the stationary phases used in reversed-phase chromatography are hydrophobic in nature. Therefore, analytes are separated by their …
[PDF File] LC Troubleshooting Series Gradients Overview Introduction …
http://5y1.org/file/23545/lc-troubleshooting-series-gradients-overview-introduction.pdf
UV Cutoff for Common Mobile Phase Components For many applications, it is ideal to have the same level of buffer or modifier in both mobile phase A and B.
[PDF File] HILIC Separations Technical Guide - Thermo Fisher Scientific
http://5y1.org/file/23545/hilic-separations-technical-guide-thermo-fisher-scientific.pdf
HILIC can be described as a variation of Reversed Phase Liquid Chromatography (RPLC) performed using a polar stationary phase. The mobile phase employed in HILIC is highly organic in nature (>60% solvent, typically acetonitrile) containing a small percentage of aqueous solvent/buffer or other polar solvent.
[PDF File] Considering Mobile Phase Compressibility to Develop a …
http://5y1.org/file/23545/considering-mobile-phase-compressibility-to-develop-a.pdf
Conclusion. Mobile phase compressibility varies within the operating space in SFC and has an impact on method robustness. Methods that operate in regions with lower compressibility are more robust than methods that operate in regions with higher compressibility. Methods with stringent robustness requirements should be optimized using pressure ...
[PDF File] Separation of 16 Cannabinoids in Cannabis Flower and …
http://5y1.org/file/23545/separation-of-16-cannabinoids-in-cannabis-flower-and.pdf
In the method presented here, the mobile phase consists of 0.1% trifluoroacetic acid (TFA) in water and acetonitrile which are mixed using the automated solvent blending capability of the WatersTM Alliance System.8 This significantly reduces variability that can happen when manually preparing mixed mobile phases.
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