Nucleotide molecular weight calculator

• [PDF File]Protein Analysis Tools on the ExPASy Server 571 52

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  (pI) and molecular weight (Mw); ProtParam, to calculate various physicochemical ... of the phrase typed in by the submitter of the underlying nucleotide coding sequence, or of a protein name inferred by automatic annotation procedures. As far as keywords (KW lines) and feature tables (FT lines) are concerned, the situation is similar: all ...

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• [PDF File]Molecular cloning, sequence identification, polymorphism ...

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  ment 2). The theoretical isoelectric point (pI) and molecular weight (Mw) of this deduced protein of this pig gene were computed using the Compute pI=Mw tool. The pI of porcine SPATS2L is 9.67. The molecular weight of this putative pro-tein is 61610.63Da. This novel porcine gene was assigned to GeneID: 100415809.

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• Useful Molecular Facts - .NET Framework

  Molecular weight of a double-stranded DNA molecule = (# of basepairs x 650 daltons) Total weight of the human genome = 3.3 x 10 9 bp x 650Da = 2.15 X 10 12 Da. One dalton

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• [PDF File]Bioinformation Volume 5 Software

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  calculator accepts the nucleotide or protein sequence then calculates their molecular weight and the number and percentage of occurrences of each residue from the given sequence. The program will ignore numbers, spaces or characters which do not correspond with one of the 4 nucleotide bases.

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• [PDF File]Nucleic Acids Research, 2007, Vol. 35, Web Server issue ...

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  conditions to calculate the length, molecular weight, estimated absorbance at 260nm, the micromolar concen- tration and micrograms of oligonucleotide present in a

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• [PDF File]Calculating Nucleic Acid or Protein Concentration

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  a few selected extinction coefficients or the Practical Handbook of Biochemistry and Molecular Biology for a more extensive table [2]. Finally, if the protein sequence of the protein to be measured is known, the theoretical extinction coefficient can be calculated using the equation ε = 5690(#Tryptophans) +

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• Small-angle scattering contrast calculator for protein and ...

  For proteins and nucleic acids, the molecular volume is estimated from the corresponding partial specific volumes using the relation V ¼ vM w =N A; ð5Þ where v is the partial specific volume of the protein or nucleic acid, M w is its molecular weight and N A is Avogadro’s number. When deuterium is present in the molecule or solution, the ...

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• [PDF File]A Guide to High Resolution Melting (HRM) Analysis

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  3 www.appliedbiosystems.com High resolution melting (HRM) analysis is a new, post-PCR analysis method used for identifying genetic variation in nucleic acid sequences.

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• [PDF File]Copyright: IPGRI and Cornell University, 2003 Measures 1

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  • Diversity and differentiation at the nucleotide level • Genetic distance fDisplaying relationships: ... of our molecular study with other types of data (e.g. geographical). In the slide, Ind ... the more weight it has when combined with other binary or categorical va riables with few categories.

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• [PDF File]A Guide to Polyacrylamide Gel Electrophoresis and Detection

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  are not suitable for molecular weight determination. Fig. 2.2. Migration of proteins and buffer ions in a denaturing discontinuous PAGE system. A, Denatured sample proteins are loaded into the wells; B, Voltage is applied and the samples move into the gel. The chloride ions already present in the gel (leading ions) run faster

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• [PDF File]Lipoprotein (a) 2010 Lipoprotein little a (small case a as ...

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  a smaller, lower molecular weight apo(a) than a person having a larger, higher molecular weight KIV-2 with 40 or more repeats. In other words, some folks have just a few copies of the KIV-2 and the next patient might have several. Thus with respect to apo(a) makeup, molecular weight and size, the most clinically important of the apo(a)

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• [PDF File]Measurement of Nucleic Acid Concentrations Using the DyNA ...

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  tions. After entry of the nucleotide sequence and concentr-a tion, it will calculate and display the molecular weight, appar - ent melting temperature (Tm) and theoretical absorbance of a given nucleic acid. The theoretical absorbance is reported as absorbance units/µmol and, like the Tm, is calculated by the

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• [PDF File]Nucleic Acids Sizes and Molecular Weights 1of2

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  Nucleic Acids Sizes and Molecular Weights Spectral Constants for Deoxyribonucleotides Compound Molecular Weight (g/mol) dATP 491.2 Average: 487.0 dCTP 467.2 dGTP 507.2 dTTP 482.2 dAMP 331.2 Average: 327.0* dCMP 307.2 dGMP 347.2 dTMP 322.2 Spectral Constants for Ribonucleotides Compound Molecular Weight (g/mol) ATP 507.2 Average: 499.5 CTP 483.2 ...

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• Nucleic Acids Research, 2007, doi:10.1093/nar/gkm234 ...

  Molecular weight calculations are based on the molecular weights available from Aldrich Chemicals, St. Louis, MO, USA. The absorbance coefficients and calculations are done as described in Molecular Cloning, a Lab Manual (4). The basic melting temperature calculation (5,6) is provided as a baseline for comparison, and is the least preferred method.

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• [PDF File]Cloning, molecular characterization, and expression ...

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  Genetics and Molecular Research 14 (3): 11154-11161 (2015) FUNPEC-RP www.funpecrp.com.br Primary and secondary structures of putative FGF5 protein The deduced goat FGF5 protein consisted of 270 residues. Its predicted molecular weight was 30,000 Da, and the estimated isoelectric point was 4.53. The putative FGF5 pro-

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• [PDF File]KaKs Calculator: Calculating Ka and Ks Through Model ...

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  KaKs Calculator Although there exist 203 time-reversible models of nucleotide substitution (8), model selection in prac-tice is often limited to a subset of them (32), andthus model averaging can reduce biases arising from

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• [PDF File]DETERMINATION OF DNA CONCENTRATION AND PURITY ¶ ¶ STUDENT ...

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  dilute the DNA 1/10 and 1/100 and run both dilutions on the gel with a molecular weight marker (MWM) of known concentration. The DNA concentration is determined by comparing it to one band in the MWM that most resembles it in brightness. See Figure 4 for a sample calculation. Figure 3. Bands of chromosomal DNA previously incubated with ethidium ...

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• [PDF File]Ariadne Mass Calculator User’s Manual

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  The Mass Calculator software calculates expected molecular weight, and m/z values of multiply charged molecular (precursor) and product ions from provided nucleic acid sequences, and under defined experimental conditions. This program calculates accurate mass values for a given nucleic acid, based on relative atomic masses of its composition

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• [PDF File]Plasmid and Protein Quantitation - Promega

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  where N is the number of nucleotide pairs and 660pg/pmol is the average MW of a nucleotide pair. For ssDNA: To convert pmol to µg: pmol × N × 330pg × 1µg = µg pmol 106pg To convert µg to pmol: µg × 106pg × pmol × 1 = pmol 1µg 330pg N where N is the number of nucleotides and 330pg/pmol is the average MW of a nucleotide.

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