Peptide synthesis procedure
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ATCC 259923. The MIC of each peptide was determined using a broth micro-dilution assay adapted from a literature procedure as previously described by Hancock.6 Peptide stock solutions were prepared at a concentration of 330 g/mL peptide in 0.2% bovine serum albumin and 0.01% acetic acid.
[DOC File]Chapter 5 Amino Acids, Peptides and Proteins
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The Edman degradation procedure labels and removes only the N-terminal residue from a peptide, leaving all other peptide bonds intact. The peptide is reacted with phenylisothiocyanate, and the N-terminal residue is ultimately removed as a phenylthiohydantoin derivative. ... The major improvement in peptide and protein synthesis is the solid ...
[DOC File]Synthesis of a Novel Ureidopeptide HIV-1 Protease Inhibitor
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The synthesis of . 1. is performed by first synthesizing each tripeptide on either side of the urea then joining via a Hoffmann rearrangement. Synthesis of each half follows standard methodology for peptide synthesis, progressing from C- to N-terminus to avoid racemization of the amino acids.
[DOC File]BREAKING THE CODE - PC\|MAC
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- Synthesis of an identical copy of a DNA strand. Transcription - Synthesis of mRNA molecule. (codon) Translation - Synthesis of polypeptides (amino acids/peptide bonds) from tRNA/ amino acids/ (anticodons) Codon - Base triplet on mRNA. Anticodon - Complementary triplet on tRNA. Polypeptide - Protein molecule . Procedure: Protein synthesis = a ...
[DOC File]ResearchGate
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Synthesis of N-Protected Peptide [NH2-Leu(1)-Aib(2)-Phe(3)-OMe] ... Methyl ester of phenyl alanine was prepared by using the same preparation procedure as used in case of H2N-Aib-OMe.
Front Matter Template - Texas Digital Library
General Peptide Synthesis Procedure: SPPS was performed according to the Fmoc standard protocol described by Chan 2000. Coupling reactions were completed in 3 hours using diisopropylcarbodiimide (DIC) and 1-hydroxybenzotriazole (HOBt). Cleavage protocols are also described in the EMD Biosciences catalogue (White 2004).
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