Peptide synthesizer
Fmoc-Cys(S-Trt)-OH - Scripps Research
Place the vial in the cavity of the microwave peptide synthesizer (Biotage Initiator) and heat the contents for 30 minutes at 100 ˚C at high microwave settings with variable power. Transfer the resin into the regular reaction vessel and wash the resin with DMF (3 mL x 10 times). Shake the resin for 1 minute between every wash.
[DOCX File]Department of Chemistry and Fermentation Sciences ...
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AAPPTEC Focus XC Automated solid phase peptide synthesizer. UVP Bio Doc-It Imaging System. UVP ChemiDoc-It. TS2. 810 Phosphoimager and thermal printer. EVOS XL Core inverted microscope. Tuttnauer 3870 ELV autoclave. Eppendorf 5424R microcentrifuge. VWR Symphony CO. 2. Incubator. Labconco Biosafety Cabinet
Core Facilities:
Peptide synthesis: A Tribute Peptide Synthesizer from Protein Technology and a Milligen 9050 Fmoc peptide synthesizer are available for peptide synthesis. A standard synthesis delivers between 50-250 mg of peptide. A standard synthesis on either of these machines delivers between 50-80 mg of peptide.
[DOC File]Biomimetic studies on the mechanism of stereoselective ...
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They were eluted in 0.1% TFA in acetonitrile/water with linear gradients optimized for each peptide with a flow rate of 1 mL/min on the analytical column and 8 mL/min on the preparative column. 1 Rainin PS3 Automated Solid Phase Peptide Synthesizer: User Guide; Rainin Instrument Company, 1998.
[DOCX File]University of Wisconsin–La Crosse
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(C) Organic and peptide synthesis: Three chemical fume hoods, automated peptide synthesizer (Advanced ChemTech, model 90), HPLC system (Waters 510 pump with tunable absorbance detector), rotary evaporator, and extensive glassware for chromatography, distillation, refluxing, etc.
[DOCX File]Resume
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Triple peptide synthesizer, Protein Technologies PS3 automated peptide synthesizer. Multiple peptide synthesizer, Advanced ChemTech, MBS 396. CEM Microwave Accelerated Reaction System (MARS), with organic reaction system. HPLC (analytic and prep) and method development, preparative Shimadzu LC-8A liquid chromatograph and . semi prep.
[DOC File]Rutgers New Jersey Medical School
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PEPTIDE SYNTHESIS. Peptides are synthesized on the . Applied Biosystems 433 Peptide Synthesizer. which is capable of producing milligram quantities of peptides up to twenty five amino acids. After cleavage and deprotection the peptides can be purified by size exclusion chromatography or by HPLC.
[DOC File]NIH SF424 Major Equipment Template:
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We have unlimited access to Applied Biosystems protein microsequenator, oligonucleotide synthesizer, mass spectrometer (capable of MS-MS applications) and peptide synthesizer in a facility run by Mr. Michael Byrne from the Dept. of Physiology. This service is provided on a pay for use basis.
[DOCX File]Lecture 1: Key Concepts in Stereoselective Synthesis
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Seeberger and co-workers first introduced an automated oligosaccharide synthesizer, which was modified from an original peptide synthesizer and optimized for automated SPOS. Seeberger Science 2001, 291, 1523. Future challenges: (1) Building blocks are used in excess
[DOC File]Dr
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Enough experience on peptide synthesis by using the peptide synthesizer. Experience on small organic molecule synthesis (one step and two step chemical reactions) Computer Skills. Experianced with different instrument software systems i.e., Masslynx, Xcalibur, Analyst and MassHunter
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