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[DOC File]Lab IV: Recombinant DNA Analysis
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C. Purify the plasmid DNA. 7. Centrifuge for 10 minutes at max speed. A white pellet will form. 8. Apply the supernatant to a plasmid prep column by decanting, or using a pipet. Your plasmid DNA will be retained on the silica-gel membrane inside due to the high salt conditions of the supernatant.
plasmid transformation protocol

[DOC File]Home | ISB Server Wahoo
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When you need a lot of high quality (pure) plasmid, do a cesium chloride prep. Under high centrifugation, CsCl2 forms a density gradient. Plasmid DNA will “float” at a particular level in the gradient and can be extracted in purified form. Confirm genotype for phenotype. E. coli …
transformation of bacteria using plasmid

[DOC File]Plasmid conformations:
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Transformation Pre-lab. The pBLU plasmid contains genes for two proteins: β-galactosidase and ampr (see figure at right). Β-galactosidase is an enzyme usually responsible for digesting sugar. However, it is able to digest substances with similar structures to a sugar, like the chemical X-gal. ...
dna transformation

DNA transformation protocol
Bacterial Transformation with “glowing” DNA (GFP on a plasmid) Bacterial Transformation begins with generating a recombinant DNA consisting of a plasmid (small circular DNA molecule distinct from the chromosomal DNA) with an inserted piece of DNA, the gene of interest (in this case GFP).
purpose of recovery transformation plasmid

[DOC File]Gene Transformation POGIL Activity
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transformation ("+ plasmid") and control ("- plasmid") suspensions onto the appropriate . LB and LB/Amp plates. Be sure to use a separate pipette for each of the four transfers. Step 16 Using a new sterile inoculation loop for each plate, spread the suspensions evenly .
bacterial transformation

[DOC File]Name:
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To test if the Transformation was successful, the plasmid is removed from the bacterium and run on a gel. By comparing the size of the plasmid to a DNA ladder, the number of base pairs in the plasmid can be determined. If the number of base pairs of the original Plasmid DNA and the Gene Insert are known, it will be easy to determine if the ...
bacterial transformation lab answers

[DOC File]AP Biology
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Media additives such as antibiotics or X-gal can select for the presence of certain genes (and therefore certain fragments) within the transformed plasmid. Transformation is a rare event, and so a single colony on selective media is in all likelihood derived from a single cell that was transformed with one species of recombinant DNA.
transformation and isolate dna protocol

Plasmid transformation