Protein secretory pathway order
[DOCX File]Chapter 04 - Cell Structure and Function
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You received partial credit if you said secretory protein without raising the possibility of a GPI anchor protein. Many of you also pointed out that the protein could be found soluble within the lumen of organelles in the secretory pathway (i.e. a resident organellar protein).
[DOC File]Question 3 (35 points): The protein humanin was discovered ...
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One example of an ER folding mechanism is the Calnexin pathway. The chaperone, Calnexin, aids in protein folding of a glycoprotein following removal of 2 glucose residues. If properly folded, the protein exits the ER and enters the secretory pathway.
[DOCX File]Ms Nickel's Biology 12
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The GFP becomes a marker for the intracellular location of the target gene product, tracking its migration by fluorescence microscopy into the nucleus, mitochondria, secretory pathway, plasma membrane or cytoskeleton. GFP can also be used as a reporter of gene expression levels as well as a measure of protein-protein interactions.
[DOC File]CHAPTER 17 FROM GENE TO PROTEIN
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After binding, the SRP leaves and protein synthesis resumes with the growing polypeptide snaking across the membrane into the cisternal space via a protein pore. An enzyme usually cleaves the signal polypeptide. Secretory proteins are released entirely into the cisternal space, but membrane proteins remain partially embedded in the ER membrane.
Cell Biology 04: The Secretory Pathway
The DNA message on the gene would be converted to RNA in the nucleus. The RNA would leave the nucleus and travel to the rough endoplasmic reticulum. At this point it would bind to a ribosome for translation to protein. The protein would leave the rough ER in a vesicle, travel to the Golgi apparatus for sorting, and then put in a new vesicle.
[DOC File]BIOTECHNOLOGY Biology W4034/3034 Final Exam Dec
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Cellular Packet. Deadline for Unit: _____ 1. Special Cells – Facebook cell._____ 2. Screencast Unit A # 1,2,3 – Watch, complete notes’ sheets._____
Abstract:
Protein synthesis resumes, with the growing polypeptide snaking across the membrane into the ER lumen via a protein pore. An enzyme usually cleaves the signal polypeptide. Secretory proteins are released into solution within the ER lumen, but membrane …
[DOC File]CHAPTER 17 FROM GENE TO PROTEIN
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For the latter, a leader signal sequence must be added so that the protein goes through the secretory pathway. In the affinity chromatography step , select for RNA molecules that bind to the HER2 domain made in mammalian cells but do NOT bind to the HER2 domain prepared from E. coli (now present in column run-through or in supernatant above ...
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