Sigma aldrich buffer calculator
[DOCX File]www.research.manchester.ac.uk
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All other chemicals were obtained from Sigma-Aldrich Co. All materials were used without further purification unless otherwise stated. ... diluted in broth. Peptide solutions were diluted two-fold using phosphate-buffered saline (10 mM phosphate buffer…
[DOCX File]Template for Electronic Submission to ACS Journals
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Gels were washed in 1 mM phosphate buffer solution (pH 7.4) with the same NaCl concentration as the pre-gelator solution (0-150 mM) at 4 °C for 1 hour, the wafer was then patted dry and stored at 4 °C …
[DOCX File]Cancer Research
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For ChIP-seq, samples were incubated 10 minutes at 4º C in Lysis Buffer 1 (50 mM HEPES , pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% IGEPAL, 0.25% Triton X-100, 1X HALT protease inhibitor) …
Supplementary Material - Max Planck Society
To induce autophagy, rapamycin (750 nM, Sigma-Aldrich) was added to the medium in selected conditions on DIV 7 for 24 hours. To evaluate a possible protective effect against cell death, staurosporine (Sigma-Aldrich…
[DOC File]Template para os Anais em CD do XX SICAT - Nathan
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S1: List of chemicals and materials for the experiment Reagents: lactose monohydrate, copper(II) sulfate, potassium sodium tartrate, sodium carbonate, sodium hydroxide, methylene blue and sodium acetate. They were bought from the Sigma Aldrich …
[DOC File]Lab manual
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Description Supplier/Source Catalog number NaCl Fisher Scientific BP358 1 red dye #40 MG Newell 07704 1 glucose Aldrich 15,896 8 1000 µS solution 495 mg NaCl/L Tracer 20 g NaCl/L. 4 g red dye …
[DOC File]Nature Research
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DEN (Sigma-Aldrich) was injected intraperitoneally in 5-month-old male mice (100 mg.kg-1 of body weight). 48 h later, animals were sacrificed and livers were immediately dissected and frozen in liquid …
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Each reaction contained 25 pmol of each primer in 1µl, 2.5 µl of 10x reaction buffer, 1.5-3µl Mg2Cl, 2.5µl bovine serum albumin (non-acetylated, BSA, Sigma B4287), 1µl of template and 0.5U of Taq …
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The column used was a XBRIDGE BEH300 C18 (3.5 µm, 2.1x100 mm) (Waters) (gradient 10-100% A). The source temperature was maintained at 120 °C and the desolvation temperature at 400 °C. The cone voltage was 40 V. The samples were injected at 0.4-1 mg/mL concentration in their respective buffer …
[DOCX File]haramaya university
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Buffer solutions were prepared from 1 mol/L sodium sulfate and 1 mol/L sodium hydrogen sulfate for pH-2, from 1 mol/L sodium acetate and 1 mol/L acetic acid for pH 3—6, from 0.1 mol/L potassium di …
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