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[DOC File]ars.els-cdn.com
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Phosphate buffer solution (PBS, 0.1 M) was prepared from NaH2PO4 (0.1 M), Na2HPO4(0.1 M) and NaCl (0.1 M). Ultrapure water (18.2 MΩ cm-1) was used throughout the experiment. Electrochemiluminescence measurements were performed on an MPI-A ECL analyzer (Xi'an Remex Instrument Co., Ltd., China).
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CsCl purified phage was transferred into 10 ml Spectra-Por® Float-A-Lyzer® G2 Dialysis membrane (10 mL, MWCO 3.5-5 kDa, Sigma-Alrich, USA) using a sterile syringe and 21 G needle (BD Bioscience). The SM buffer was replaced after 12 hrs and the salinity of the concentrating solution was measured using a refractometer to ensure sufficient ...
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[DOC File]Materials and Methods - Nature Research
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Propidium iodide (Sigma) or DAPI (Invitrogen) was used to distinguish live cells from dead cells during cell analysis and sorting. Stained cells were analyzed on a FACS Canto or LSRII machine using Diva program (BD Bioscience) or purified with a MoFlo Astrios cell sorter (DakoCytomation). Cells were > 98% pure after sorting.
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[DOC File]Knowledge Check Worksheet
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“The tools common to all quality efforts, including Six Sigma are flowcharts, run charts, Pareto charts, histograms, checksheets, cause-and-effect diagrams, and control charts” (Chase, 2011, p. 293). Reading: Ch. 9 of Operations and Supply Chain Management “Analytical Tools for Six Sigma and Continuous Improvement” p. 293-296
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[DOC File]16S rDNA GeneChip Protocol 11/18/98
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10 mls Triton X-100 (Sigma-Aldrich) Final Volume: 100 mls. Mix thoroughly and store solution at room temperature. 4. Wash Buffer A: 1X SSPE/ 0.005% Triton X-100. 100 mls 50 mls. 5 mls 20X SSPE (BioWhit) 2.5 mls. 50 (l 10% Triton X-100 Stock Soln 25 (l 94.95 mls …
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[DOC File]Flow chart p - Harvard University
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0.1M Acetosyringone in methanol (= 0.0196g/mL) (a.k.a. 3’5’Dimethoxy-4’-hydroxyacetophenone, Sigma cat# D134406) Note: Make 40mL or larger quantities of MgCl2 and MES stock solutions and filter sterilize them. Store MgCl2 at room temperature and store MES in aliquots at -20 deg C. For acetosyringone, make a small quantity (usually 3 or 4 ...
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Sigma buffer chart