Spps resin
[DOCX File]Tetrahedron template - Imperial College London
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Bu solid-phase peptide synthesis (SPPS), and the warhead moiety coupled directly to the N-terminal amine prior to cleavage from the resin as a C-terminal PEG-Biotin conjugate. N-terminal chloroacetamide (CAA), fluoroacetamide (FAA) and Michael acceptor (MA) probes were readily synthesized in acceptable overall yields, with a range of ...
[DOCX File]Scitation - AIP Publishing
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The biotin was coupled into the N-terminus of assembly peptide with SPPS method using the assembly peptide before cleavage of peptides from resin, the SPPS method, a microwave peptide synthesizer, and Fmoc chemistry. The crude products were cleaved from H-rink Amide-ChemMatrix® resin (0.53 mmol/g, PCAS) in the solution of trifluoroacetic acid ...
[DOCX File]Table of contents - ResearchGate | Find and share research
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Resin-bound peptide (Boc-GGGY(tBu)K(Alloc)-Rink Amide resin) was synthesized manually via standard solid phase peptide synthesis (SPPS) using Rink amide AM resin …
Front Matter Template
Instead, with SPPS, the resin is simply separated by filtration, where the remnants of the reaction mixture are easily removed by washing the resin with N,N-dimethylformamide (DMF) and/or Methanol (MeOH). To preserve the specificity of the peptide elongation reactions, protecting groups are necessary to prevent undesired reactions from taking ...
[DOCX File]Welcome to CaltechAUTHORS - CaltechAUTHORS
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Bulk synthesis of PCC candidates was carried out on 2-chlorotrityl chloride (CTC) resin (1.04 mmol/g) using microwave-assisted Fmoc-based solid-phase peptide synthesis (SPPS). The first amino acid was attached to the resin following the vendor’s protocol. The resin was transferred to the CEM Liberty 1 for the coupling of the remaining amino ...
[DOCX File]Lecture 1: Key Concepts in Stereoselective Synthesis
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Today, SPPS is acknowledged as the method of choice for creating peptides in a synthetic manner. In the most common strategy the C-terminus of an amino acid (with protected side chain and N-terminus) is immobilized on a solid support (e.g. cross-linked polystyrene resin).
[DOCX File]Normal.dot - Osteoarthritis
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A solution of Grubbs 1st generation catalyst (4 mg/mL) in DCE was added to the resin and reacted for 1 h at room temperature. During reaction, nitrogen was bubbled through the mixture. The procedure was repeated five times and the resin was washed in a DMSO/DCM (1/1) solution for 10 min followed by a washing step in DCM.
[DOC File]ars.els-cdn.com
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All peptides were prepared by Fmoc SPPS methods using Rink amide resin with an initial loading of 0.61 mmol/g, unless otherwise noted. Resins were swollen in N,N-dimethylforamide (DMF) for 45 …
[DOCX File]Centers for Disease Control and Prevention
https://info.5y1.org/spps-resin_1_407e05.html
The resin was subsequently washed three times in DMF (3 x 5 min) prior to coupling the amino functionality of the resin to Fmoc-Gly-OH using 5 molar equivalents (relative to resin) of Fmoc-amino acid, 5 equivalents of (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate (PyBOP), 5 equivalents of hydroxybenzotriazole (HOBt) and 13 ...
[DOC File]Supporting information
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The resin containing Boc-Alg-O-ArgoGel was washed with DMF (3 ( 10 mL, 2 min), and CH2Cl2 (3 ( 10 mL, 2 min). The obtained resin was swelled in 1,1,2-trichloroethane (20 mL), Fmoc-Alg-OH (768 mg, 2.28 mmol) was added and the reaction mixture was purged with N2 at 60oC for 20 min.
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