Stock dilution calculator
[DOC File]Calculations
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Rather than reaching for the formula and the calculator, let’s use the principles above. A 0.01 M solution is equivalent to 10 mM and contains 10 mmol/L or 10 mol/mL ( 5 mol in 0.5 mL. This 5 moles is now in a final volume of 20 mL ( 5 mol/20 mL ( 250 M or 0.25 mM. ... If you are going to use stock solutions to make up buffers, prepare assays ...
[DOC File]Buffers and stocks - University of Pittsburgh
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Make LB/ agar as described above. Place in 55˚C water bath. After temperature has adjusted to 55˚C, add ampicillin from a 100mg/ml stock to a final concentration of 100µg/ml. Pour plates. 20xSSC [3 M NaCl, 0.3 M Na citrate pH 7] 2 l. NaCl 350.64 g. Na citrate, 2H2O 176.46 g. 1 M Na2CO3 50 ml. Na2CO3 (anhydrous) 5.3 g. Water until 50 ml
[DOCX File]University of Miami
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A tenfold serial dilution (dilution factor of 10). Note that at each step, the previously diluted solution provides 10% of the solution in the next step. Other dilution factors can be used, as well. This procedure can be used to produce an extremely dilute reagent, depending on how many dilution …
[DOC File]DILUTIONS AND STANDARDS
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Stock standard = 10 mg/mL x 100 mL/1dL = 1,000 mg/dL. 2. To determine the dilution that must be made to yield a working standard of the desired concentration, we use the following equation: desired concentration of working std./concentration of stock std. = dilution required. Using the information from our example, we now see: 100 mg/dL = 1/10
[DOCX File]Welcome to web.gccaz.edu
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M. 2 is the final concentration or molarity after dilution (must be lower than M 1) V 2 is the total volume of all solutions and liquids Note: Concentrations and Volumes can be in any units as long as they are the same before and after, i.e., both volumes in mL, drops, L, etc. and both concentrations in M, mg/mL, etc.
Fish 424 Laboratory
Calculator. Procedure . ... of your viral stock. Average # Plaques = PFU/ml D = Dilution factor. D x V V = Volume of diluted virus ... This will give you the dilution of the original suspension ...
[DOC File]USING A CONDUCTIVITY PROBE
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Complete all calculations for Dilution Procedure #1 as follows: Use the equation C1V1 = C2V2 to calculate the amount of 1000 ppm NaCl stock solution required to make 50 mL each of 200 ppm, 150 ppm, 100 ppm, 50 ppm, and 25 ppm NaCl solutions.
[DOC File]Comparing Mitosis and Meiosis
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Calculator. Procedure. Part A. Making Metric Conversions. ... A concentrated solution is generally called a “stock solution,” and the diluted solution is called the “working solution.” Preparing a concentrated stock solution saves a lot of time and is easier to store than large volumes of diluted working solutions. ... A serial dilution ...
[DOC File]Mx4000 – Standard Operating Procedure
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For new users a serial dilution of the sample may be necessary. An example for creating 1, 10 and 100 ng/rxn follows: Determine the DNA concentration in the sample by measuring absorbance at A260 or by using a dsDNA quantification kit such as Quant-iT DNA Assay Kits (Molecular Probes #Q33120 or #Q33130) or PicoGreen (Molecular Probes #P7589).
[DOC File]QUALITY ASSURANCE MANUAL
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Dilution water is prepared by adding one mL of each nutrient solution per liter of reagent water. Dilution water should be allowed to equilibrate in the incubator or in the lab for at least 24 hours at room temperature (68-72(F or 20-22(C). It is important that dilution water be saturated with oxygen.
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