What is a 1 1 dilution
[DOC File]Molarity and Dilution Lab
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Part 2: Dilution. Your lab group must make 100 mL of a M CuSO4·5 H2O solution using the stock solution you made in Part 1. Pour your stock solution out of the volumetric flask into the empty 250 mL beaker. Using the dilution formula, calculate the number …
[DOC File]Dilution Worksheet( Homework) Mrs
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M1V1 = M2V2 Lowell High School. 1. A stock solution of 1.00 M NaCl is available. How many milliliters are needed to make 100.0 mL of 0.750 M
[DOC File]DILUTIONS AND STANDARDS
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A 1:10 dilution of serum was made by adding one part serum to nine parts diluent to make a total of ten parts. If 1.0 milliliter of serum is added to 9.0 milliliters of H20, a total volume of 10.0 milliliters is obtained. Therefore, the dilution is expressed according to the following equation.
[DOCX File]A1.1.5.ELISA
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Under the diagram, calculate the dilution in each well. Think about what you have added to each well and about the practice serial dilution questions you completed in Part II. Note that the concentration of antigen in well #1 is 1000 ng/ml. Calculate the concentration of antigen in wells 2-12.
[DOC File]DILUTION PROBLEMS:
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Then 1 mL of that was diluted to 8 mL. What was the final dilution of serum? You took 4 mL of a glucose solution and diluted it to 10 mL. Then a 1:10 dilution was made of that. What was the final dilution? In performing a serological procedure, you add 1 mL of serum to 5 mL of saline. A 1:2 serial dilution …
[DOC File]A1.1.5.ELISA
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Under the diagram, calculate the dilution in each well. Think about what you have added to each well and about the practice serial dilution questions you completed in Part II. Note that the concentration of antigen in well #1 is 1000 ng/ml. Calculate the concentration of antigen in wells 2-12. Show your work on your sheet of paper.
[DOC File]Lab One
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: pipet 1.0 ml milk into above dilution tube, vortex to mix. Add aliquots: First pipet. 1.0 ml into two plates (1:100) and then pipet 0.1 ml into two plates (1:1000) Get another 99 ml sterile . dH2O. Pipet 1.0 mL milk from 1: 100 dilution bottle into above dilution tube, vortex to mix. Add 1.0 ml aliquots: Using a pipet, pipet . 1.0 ml into two ...
[DOC File]Biology Tests and Procedures | Biology Junction
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In a serial dilution the total dilution factor at any point is the produce of the individual dilution factors in each step up to it. Final dilution factor (DF) = DF1 * DF2 *DF3 etc Example: If one were wanting to perform a three step 1:100 serial dilution of bacterial culture, the initial step combines 1 unit volume culture ( 10ul) with 99 unit ...
[DOC File]Quiz I
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The OD595 of the undiluted protein is 1.673. The OD595 of a 1:10 dilution of your protein is 1.242 . What is the concentration of your protein (show your calculations)? [(1.242-0.1196)/.9429] *10 = 11.904 mg/ml. If you diluted your protein 1:5, what would be the expected OD595? 11.904/5= 2.38 mg/ml; Outside of standard curve. Reached saturation ...
[DOC File]dilutions
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Then adding 1 mL of this solution to 9 mL of solvent in another test tube produces a 1/1000 dilution of the original solution. Here's that rule again for doing serial dilutions, you multiply the dilution factors: 1/10 X 1/10 X 1/10 = 1/1000.
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