Where do you find dna
[PDF File] Ask A Biologist - Banana DNA Extraction - Activity
http://5y1.org/file/7180/ask-a-biologist-banana-dna-extraction-activity.pdf
Extracting DNA from a in 10 Easy Steps. Before you begin - Make sure the alcohol (isopropyl alcohol) is in the freezer getting cold and check each box after you complete each step. Remove the banana peel and mush half of the banana in the zip bag for 1 minute. . Add the saltwater to the bag and close it.
[PDF File] Advocating for the Fair - Office of Justice Programs
http://5y1.org/file/7180/advocating-for-the-fair-office-of-justice-programs.pdf
Three types of results can occur in DNA testing: inclusion, exclusion, and inconclusive. It is important that victim service providers understand the meaning of these terms and be able to explain their implications. Inclusion. When the DNA profile of a victim or suspect is consistent with the DNA profile from the crime scene evidence, the ...
[PDF File] Pre-lab Homework Lab 3: DNA Structure and Function
http://5y1.org/file/7180/pre-lab-homework-lab-3-dna-structure-and-function.pdf
3. The four nucleotide monomers of DNA are made up of 3 smaller molecules joined together. These 3 smaller molecules - a phosphate group, a deoxyribose sugar, and a nitrogenous base - are covalently bound together to build each nucleotide. Draw one nucleotide and label each of the 3 parts.
[PDF File] LESSON DNA Profiling Using STRs Educator Materials
http://5y1.org/file/7180/lesson-dna-profiling-using-strs-educator-materials.pdf
In Part 1 of this lesson, students learn the basics of DNA profiling, including the structure and inheritance of STRs. In Part 2, students learn how DNA profiles are compiled with STRs that are typically used in forensic investigations. In Part 3, they work through a case study involving a robbery and build a DNA profile that can be compared to ...
[PDF File] DNA Evidence: What Law Enforcement Officers Should Know
http://5y1.org/file/7180/dna-evidence-what-law-enforcement-officers-should-know.pdf
degradation (or erosion) of DNA. As a result, not all DNA evidence yields usable profiles. (See “Safeguard DNA Evidence and Yourself.”) Officers should not drink, eat, litter, smoke, or do anything else that might compromise the crime scene. They should remember that valuable DNA evidence may be present even though it is not visible. For ...
[PDF File] The DNA Files: Workshops and Activities - Exploratorium
http://5y1.org/file/7180/the-dna-files-workshops-and-activities-exploratorium.pdf
• DNA is a set of instructions needed to make a living thing. 1. Ask participants for their ideas about where in the body they might find DNA. 2. Take suggestions and write them on a blackboard, whiteboard or flipchart. (Blood has DNA, of course, but not in the red blood cells—in the white blood cells. Pretty much every
[PDF File] Extracting DNA from a Banana and Other Fruits - Calbio Research
http://5y1.org/file/7180/extracting-dna-from-a-banana-and-other-fruits-calbio-research.pdf
Extracting DNA from A Banana. Mush the banana in the resealable bag for about a minute until all the lumps are gone and it almost looks like pudding. You can do this also with other soft fruit that you can mush up like strawberries. Mix the hot water and salt in a cup. Pour the saltwater mix into the bag.
[PDF File] Experiment #6: DNA Extraction from Fruits - #hayalinikeşfet
http://5y1.org/file/7180/experiment-6-dna-extraction-from-fruits-hayalinikeşfet.pdf
DNA contains the instructions needed for an organism to develop, survive and reproduce. In order to carry out these functions, DNA sequences are converted into messages that can be used to produce proteins, which are the complex molecules that do most of the work in our bodies. The Messages of the DNA are used to make proteins in a two-step ...
[PDF File] A Practical Guide to Analyzing Nucleic Acid Concentration and …
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5. RNA contamination in DNA samples 6. Measuring NA in water or buffer Nucleic acid concentrations are determined by measuring the absorbance of ultraviolet light. Derived from the Beer-Lambert law, the amount of light absorbed at 260 nm is proportional to the concentration of nucleic acid in solution. Extinction coefficients have been determined
[PDF File] The DNA detective game - Science in School
http://5y1.org/file/7180/the-dna-detective-game-science-in-school.pdf
national DNA database (Figure 4b). You may choose to let the investiga-tors compare their samples against the database, even if they have identified a murder suspect. Investigators: can you find a match between the evidence collected at the crime scene and the profiles in the DNA database (Figure 4b)? Who do you think is the murderer? 10.
[PDF File] qPCR Quantification Protocol Guide - Boston University
http://5y1.org/file/7180/qpcr-quantification-protocol-guide-boston-university.pdf
2. Put the optical strip lids on the wells and briefly spin the 96-well plate down in a bench top centrifuge to 280 xg for 1 minute. 3. Place the 96-well plate in the qPCR machine in the correct orientation and clean the optical lids with lens tissue to remove any dust before closing the qPCR machine lid. 4.
[PDF File] comparing DNA - Exploratorium
http://5y1.org/file/7180/comparing-dna-exploratorium.pdf
has DNA, of course, but not in the red blood cells – in the white blood cells. Pretty much every other cell in the body has DNA, including bone cells, organs, and skin. The hair shaft is made up of dead cells, which do have DNA, but it isn’t usually good quality unless you also get the root of the hair, the follicle. There is no need
[PDF File] DNA isolation from bananas – kitchen experiment
http://5y1.org/file/7180/dna-isolation-from-bananas-kitchen-experiment.pdf
Deoxyribonucleic acid (DNA) is the carrier of heritable information in all living organisms. In humans, DNA can be found inside almost every cell of the body. As in bananas, DNA is located within the cell in an even smaller com-partment, the cell nucleus. With a few simple household items, you can isolate the DNA of bananas in your kitchen.
[PDF File] Solutions to 7.012 Problem Set 5 - MIT OpenCourseWare
http://5y1.org/file/7180/solutions-to-7-012-problem-set-5-mit-opencourseware.pdf
Suppose you find that the harE gene is in the plasmid, but now you want a restriction map of the recombinant plasmid. You take three individual samples of the plasmid and digest one sample with EcoRI, the second sample with HindIII, and the third sample with both EcoRI and HindIII. Then you run the digested DNA on a gel to see the fragments.
[PDF File] DNA Collection Using Semen Samples - Orivet
http://5y1.org/file/7180/dna-collection-using-semen-samples-orivet.pdf
We can extract DNA from a semen straw or pellet. The straw or pellet can be shipped at room temperature – no liquid nitrogen is required. Let the sample thaw in the fridge and store at 4°C before shipping. Leaving the sample at room temperature for long periods can cause mould to appear on the sample. Place the specimen in a plastic zip lock ...
[PDF File] maY 2021 How can we find out about ancient Egyptian germs?
http://5y1.org/file/7180/may-2021-how-can-we-find-out-about-ancient-egyptian-germs.pdf
DNA – deoxyribonucleic acid. DNA is a complex molecule that contains genetic code. The genetic code in DNA contains the instructions for making an organism. DNA sequence – the exact order of the bits of DNA. DNA sequencing is the process of working this out. Environmental microbiome – the set of microorganisms living in soil, air, and water.
[PDF File] DNA Structure - Dolan DNA Learning Center
http://5y1.org/file/7180/dna-structure-dolan-dna-learning-center.pdf
What does DNA stand for? 2. Where do you find DNA in living things? 3. What is the function of DNA? During-lab observations: Label the DNA model diagram below. P = Phosphate, S = Sugar (deoxyribose), A, T, C, G = Bases Post-lab questions: 1. What are the 4 bases in DNA? How do these bases pair up? 2. What does DNA need to do in order to ...
[PDF File] Identifying Victims Using DNA: A Guide for Families (Brochure)
http://5y1.org/file/7180/identifying-victims-using-dna-a-guide-for-families-brochure.pdf
In many cases, DNA testing is one of the best methods to identify a victim or victims. DNA is the material in cells that stores the inherited traits that make up our bodies. In many (but not all) cases, DNA can be isolated from human remains or other samples. To identify the remains of a victim, DNA from remains found at the disaster or ...
[PDF File] Interpretation of Nucleic Acid 260/280 Ratios - Thermo Fisher …
http://5y1.org/file/7180/interpretation-of-nucleic-acid-260-280-ratios-thermo-fisher.pdf
purity in both DNA and RNA extractions. A 260/280 ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA. Common Problems . Abnormal 260/280 ratios usually indicate that a sample is contaminated by residual phenol, guanidine, or other reagent used in the extraction protocol, in ...
[PDF File] Dolan DNA Learning Center Restriction Analysis
http://5y1.org/file/7180/dolan-dna-learning-center-restriction-analysis.pdf
Mini Lesson 2 Gel Electrophoresis. Answer the following questions based upon the diagram of the gel seen below. 1. Label the positive and negative electrode side of the gel. 2. Draw an arrow on the gel in the direction the DNA will move and explain why it will move in that direction. 3. Label the wells from left to right with the following symbols:
[PDF File] Cells and DNA - MedlinePlus
http://5y1.org/file/7180/cells-and-dna-medlineplus.pdf
This is critical when cells divide because each new cell needs to have an exact copy of the DNA present in the old cell. DNA is a double helix formed by base pairs attached to a sugar-phosphate backbone. (Figure 8) FIGURE 8: DNA is made up of base pairs and a sugar phosphate backbone.
[PDF File] Calculating Nucleic Acid or Protein Concentration - Promega …
http://5y1.org/file/7180/calculating-nucleic-acid-or-protein-concentration-promega.pdf
When using a 1 cm cuvette, the pathlength is 1 and equation. 1 can be simplified to OD x Extinction Coefficient x sample dilution. For example, if an undiluted dsDNA sample measured in a 1 cm cuvette gives an OD 260 value of 0.9 OD, the dsDNA concentration would be calculated as: 0.9 OD 50 45 μg/ml DNA.
[PDF File] Solutions for Practice Problems for Recombinant DNA, Session 4: …
http://5y1.org/file/7180/solutions-for-practice-problems-for-recombinant-dna-session-4.pdf
market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the harE protein) that stimulates hair growth. You construct a fungal genomic DNA library in E. Coli with the hope of cloning the harE gene. If you succeed you will be a billionaire! You obtain DNA from
[PDF File] DNA Extraction Experiment - MIT Department of Biology
http://5y1.org/file/7180/dna-extraction-experiment-mit-department-of-biology.pdf
If you want to keep the DNA for further experiments: 13. Dunk several time the spooled DNA into a small tube containing clean alcohol and let air dry. 14. At this point you can store the DNA in sterile water in the freezer. Notes: • If you do not open the lid while the wheat germ is settling, carbon dioxide will build up in the tube
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