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[DOC File]Appendix A - Harvard PlasmID Database
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Leave blank after . provide for a description of points of biological significance in the sequence. M, under the following conditions: if "n," "Xaa," or a modified or unusual L-amino acid or modified base was used in a sequence; if ORGANISM is "Artificial Sequence" or "Unknown"; if molecule is combined DNA/RNA.
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[DOC File]Real time quantitative PCR
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Sequencing primers (M13F and T7 Rev) that prime from outside of the attL sites are generally suitable for sequencing inserts larger than ~500 nt, but they may provide incomplete sequences for smaller inserts, due to L1-L2 hairpin formation (Esposito et al. Biotechniques 35:914, 2003). In contrast, the use of sequencing primers GW1 and GW2 ...
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[DOC File]Primers - NGRL
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Sequencing primers (M13F and T7 Rev) that prime from outside of the attL sites are generally suitable for sequencing inserts larger than ~500 nt, but they may provide incomplete sequences for smaller inserts, due to L1-L2 hairpin formation (Esposito et al. Biotechniques 35:914, 2003). In contrast, the use of sequencing primers GW1 and GW2 ...
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[DOC File]Homemade Primer Course - AardvarkReloading
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RT-PCR method and primers used. Twelve labs reported the use of random hexamers for reverse transcription. The final concentrations ranged from 6µM - 125µM. One lab used a proprietary method and another lab did not provide information. The amount of RNA added to the cDNA reactions varied from 0.3 – 10 µg. Standard type and source
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[DOC File]Appendix A - SIGnAL
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Yq11.223 - 323 - 381 red STR Marker information supplied in QST*R-XY. STR marker, chromosome locations, amplified allele size ranges, fluorescent label and observed heterozygosity. Marker Chromosome Location Observed Heterozygosity Allele Size range (bp) Marker Dye Colour D13S252 13q12.2 0.85 260 – 330 red D13S305 13q13.3 0.75 418 – 470
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[DOC File]Primers - NGRL
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Random primers 1. dd H2O 2. 6 ul. Once ready, heat at 70C for 10 min, then place on ice for a while, followed by adding: 5x reverse tran. Buf. 2. 0.1 M DTT 1. dNTP 0.5. reverse transcriptase 0.5. 10 ul. Once ready, incubate it at RT for 10 min, then at 42C for 1-2 h.
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Which primer for .223? | The High Road
These primers have successfully set off powders as slow as IMR 4895 used in 223 Remington cartridges. The cost of making cap primers is quite low ($0.003/primer) compared to commercial primers ($0.04/primer), or about a 10:1 cost advantage. Primers made from strike-on-the-box matches. This method is easy, relatively safe and easy.
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Which primers for 223