ࡱ> PROq` P&bjbjqPqP .F::*%"   8> T $"! " f!h!h!h!h!h!h!$"hA%! ! !   f! f!V& @ f  f f!!0!r x%% % |  !! ! """ """ """ Dereks Ocular Microbiology Questions Can an opportunistic pathologenic agent go through a healthy skin? No, the normal flora will not penetrate through an uninterrupted skin. (It is the only one) What kind of bacteria life in a normal conjunctiva? Staphylococcus What would be the mechanism of adhesion to the surface? Protein, pili, fimbriae What could be protective for the formation of an infection in the eye? Lysozyme, blinking, bacteriocins Typical ocular infection with Gram negative that you see in two things: a) mascara b) hospitalized patient Which of the following would not be in a normal conjunctiva flora? a) Staphylococcus aureus b) Fungus c) Virus d) Escherichia coli Answer c Which one is the most prevalent protein in tear film? Lysozyme Which Ig is most prevalent in the tears? IgA Which Ig is most prevalent in the body? IgG Which is the most prevalent antimicrobial protein in the tear film? lactoferrin, betalysine. IgA What do we see more common in the eye? a) Aerobic b) Anerobic Answer a Which of the following conditions is not caused by Staph? a) parinauds oculoglandular conjunctivitis b) Meibomitis c) Blepharitis Answer a Rochalimaea hanselae is the most common agent of the a) Blepharitis b) Meibomitis c) Parinauds oculoglandular conjunctivitis/ Answer c Which one of the infestation is the most common in the eye? a) Pediculus humanus corporis b) Pthirus pubis c) all of them can infect the eye Answer b How many weeks is the chronic conjunctivitis? more than 4 weeks Which organism cause chronic conjunctivitis? Staphylococcus aureus, Maraxella lacunata, Streptococcus pneumoniae, Heamophilus influnenza Haemophilus aegyptius Which of the following does not cause an acute purulent conjunctivitis? a) Staphylococcus aureus b) Streptococcus pneumoniae c) Haemophilus influenza d) Actinomyces israelii Which organism can invade the intact corena? Neisseria gonorrhoeae and Pseudomonas aerginosa Different between Pseudomembranes, Membranes and Cicatrical pemphigoid? - Pseudomembranes: Membrane can move - real Membranes: Membranes can not move - Cicatrical pemphigoid: attachment between lid-conj. Which of the following does not produce a pseudomembrane? a) Streptococcus pyogenes b) Neisseria gonnorhoeae c) Heamophilus influenzae d) Corynebacterium diphtheriae Answer d The kimpura spatula is used for isolation of: a) viruses and chlamydia trachomatis b) pseudomaonas infection c) corneal infection d) all of the above Answer a Which stain is used for Chlamydia and adenoviruses? Ziehl neelsen acid fast stain or Immunofloresence staining Immunoflorecence is a method used for: Chlamydia trachomatis and viruses What do we stain particulary with immunofloresence? reticulate bodies of chlamydia trachomatis Which is the most frequent cause of viral conjunctivitis? Adenoviruses Which adenovirus is more seen in school aged children? a) EKC Epidemic keratoconjuinctivitis b) PCF Pharyngo conjunctival fever What could cause a acute follicular conjunctivitis? Influenza A virus, Newcastles disease virus, Cytomegalovirus, Adenovirus, Primary Herpes Simplex virus, Enterovirus, Toxic:-Molluscum contagiosum Which organism has a more severe presentation in the eye? a) Virus b) Fungus c) Gram positive d) Gram negative Answer d Which organism can perforate the eye? Pseudomonas aeruginosa and Neisseria gonorrhoeae Which is the most common cause of fungal keratitis? a) Fusarium b) Candida c) Aspergillus Answer c Which is the cause of keratitis associated with therapeutic contact lenses? Candida Differentiation between cases with Herpes Simplex versus Herpes Zoster? Where are the lesions? age of the patient history of other head involvement Fungal stains pink a) Ture (yeast stain violet, bacteria stain dark blue) b) False Answer a You are suspecting that your patient has an infection with microbacterial tuberculosis (or fungal cases) which stain would you use? Ziehl-Neelsen stain for micobacterial Gomori Methenamine Silver (GMS) for fungal If you make a fungal stain and you see the fungal magenta colours, what stain do you use? Periodic Acid Schiff (PAS) stain What is the most common causes of the post-operative Endophthalmitis? a) Gram positive b) Gram negative c) Fungus d) Virus Which organism is the most common cause of endogenous endophthalmitis? a) Staphilococcos aureus b) Saphilococcus epidemidis c) Heamophilus influenzae d) Candida albicans Which is the most common cause of a) blepharitis Staphylococcus aureus b) canaliculitis Actinomyces israelii c) hordeolum Staphylococcus aureus d) chalazion Staphylococcus aureus e) Meibomiantis Staphylococcus aureus f) angular blepharitis Moraxella lacunata What are pili? it is a bacteria surface protein typically in Gram neg. has an important role for adhesion an the cellsurface Which organism can you get to exposure to contaminated water and organic matter? Acanthamoeba If you taking a sample should the patient be on an antibiotic treatment? a) yes b) no Answer b Which organism can cause a) Bleb induced entophthalmitis: Streptococcus spp. Haemophilus influenzae b) Post operative entophthalmitis: Staphylococcus aureus c) Endogenous entdophthalmitis: Fungal, Candida albicans Which one is the most visual developing? Penedrating Injurys Which is the most common form of entophthalmitis? Post operative enophthalmitis Cause of Acute conjunctivitis: Staphylococcus aureus Streptococcus pneumoniae Heamophilus influenzae Cause of Hyperacute conjunctivitis: Neisseria gonorrhoeae Neisseria meningitides Cause of Chronic conjunctivitis: Staphylococcus aureus Moraxella lacunata What is the most common antimicrobial protein in tears? Lysozyme The conjunctival scraping is used for: Chlamydia trachomatis Viruses Difference between the EKC (Epidemic Keratoconjunctivitis) and the PHF (Pharyngo cnjungtival Fever) PCF: fever, pharyngitis (throat), conjunctivitis, usually in children and young adults Which bacteria (Gram staining) make a diffuse rapidly spreading necrotic lesion in the cornea? Gram negative (Pseudomonas aeruginosa? Streptococcus penumoniae?) Difference between Hypopyon and Hyphema? Hypopyon: white blood cells in the anterior chamber Hyphema: red blood cells in the anterior chamber Which is the most common cause of fungal keratitis? Trauma with an organic matter (branch of a tree) Which is the different in the corneal appearance between the herpes simplex virus and the herpes zoster virus? HSV has terminal bulbs in dentrites What is caused a secondary glaucoma in Herpes Simplex virus? trabeculitis SDA cultures is used for: Fungus (Sabourauds Dextrose Agar) NNA cultures is used for: Acantamoeba (Non Nutrient Agar) Which organism caused exogenous endophthalmitis? Staphylococcus aureus (Gram positive) Which organism caused endogenous endophthalmitis? Fungal, Candida albicans     PAGE  PAGE 1 %&'imo    P h i yhyVE7h+WOJQJ^JmH sH  h%h%OJQJ^JmH sH #hcB*OJQJ^JmH phsH  h%hWOJQJ^JmH sH #h%B*OJQJ^JmH phsH h%OJQJ^JmH sH #hWB*OJQJ^JmH phsH )hWhWB*OJQJ^JmH phsH hWOJQJ^JmH sH  hWhWOJQJ^JmH sH hWOJQJ^JhOJQJ^JhWhWOJQJ^J&' i G A r  ? }j & F 8h\ @^`gd) & F 8h\ @^`gdV & F 8h\ @^`gd"E & F 8h\ @^`gdW*&O&    F G 7 @ A m q r   1 ; ? 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