ࡱ> KMJ @ *"bjbjʚʚ (B*    4>Dn2iii$Riiiii(i:i:,m @Fyd@ " 9>0nRmmiiiiiii   Photosynthesis and Cellular Respiration: Making the Connection Background Information: Green plants use the energy in the suns rays to make food. The production of food also requires raw materials. When plants synthesize food (more precisely, carbohydrates), they use carbon dioxide and water. The process of synthesizing carbohydrates with the aid of the energy in light is known as photosynthesis. The carbohydrates plants make are used by plants and animals alike as a source of energy. To release the energy contained in the bonds of carbohydrates molecules, the chemical reaction of photosynthesis must be reversed. The process in which energy is released from food is called respiration. Respiration also produces waste products carbon dioxide and water which are the same substances that served as raw materials for photosynthesis. Photosynthesis6H2O (Sunlight) +6CO2 (C6H12O6+6O2C6H12O6 + 6O2( 6CO2 + 6H2OCell Respiration In water, carbon dioxide dissolves to form a weak acid. As a result, an acid-base indicator such as bromthymol blue can be used to indicate the presence of carbon dioxide. The purpose of adding sodium bicarbonate powder to the water increases the amount of carbon dioxide in the water. In this laboratory investigation, you will be using bromthymol blue to explore the relationship between photosynthesis and respiration. Problem: What is the relationship between the processes of photosynthesis and respiration? Materials per group: 2 test tubes Test tube rack Dechlorinated water (room temp) Sodium bicarbonate (baking soda) Meter stickBromthymol blue solution 2 springs of Elodea Light source (40 watt) Tape Scissors Straws Procedure: PART A. Setting up the Experiment 1. Obtain an approximately 1 inch sprig of elodea. Remove several leaves from around the cut end of the stem. Slice off a portion of the stem at an angle and lightly crush the cut end of the stem. 2. Place a sprig of the plant into the each test tube, stem end up, filled approximately half full with water. 3. Label the test tubes as either Dark or Light 4. To each test tube add 5-7 drops of bromthymol blue. Just enough to change the water color. 5. . Into the both test tubes, take a straw and gently blow some CO2 bubbles, careful not to splash the water and bromthymol blue solution. Note your observations under your data table. 6. Place the LIGHT test tube in a test tube rack to continue on with PART B; place the DARK test tube in a test tube rack in the front for your teacher to place in a dark spot. PART B. Running the Experiment 1. Place a 40 watt lamp 5 cm from the test tube containing the Elodea. Wait one minute, count and record the number of oxygen bubbles rising from the cut end of the stem. Count bubbles in one minute intervals for five minutes straight. If bubbles fail to appear, cut off more of the stem and re-crush. 2. Run a second five-minute trial. Record and average your results. 3. Move the lamp so it is 20 cm from the plant. Wait one minute, count and record bubbles for two five-minute trials. Again, average and record your results. 4. Add a pinch of sodium bicarbonate powder to the test tube. Once again place the lamp 5 cm from the test tube. Wait one minute, then record bubbles for two five-minute trials. Average and record your results. 5. Reacquire the DARK test tube from your teacher, record any solution color change observations. Compare this to the test tube you used to record bubbles with. Did its solution have any change in color? Once again record your observations. 6. Prepare a bar graph of your results. Use the average number of bubbles for the vertical axis. Use the type of environmental condition for the horizontal axis. CAUTION: Make sure the light bulb is not touching the side of the cardboard housing, it becomes very hot and may start to burn! Data: Create your own data table Conclusion and Analysis Questions: What was the color of the bromothymol blue solution before you exhaled into it? After you exhaled into it? What was the color of the bromthymol blue solution in the test tube that was placed in the dark for a period of time? In the test tube that was placed in the light? What substance was released into the bromthymol blue solution when you exhaled into it? How is this substance produced? Why did the color change? Why were the results for the test tube placed in the light different from the test tube placed in the dark? Why was Elodea placed in both test tubes? Compare the rate of photosynthesis between the 5cm distance and 20cm distance. Was there a difference? Explain. How does the rate of photosynthesis change when sodium bicarbonate is added to the water? (increases?/decreases?) How are photosynthesis and respiration related? Explain the results of the following experiment: Carbon dioxide was bubbled through two flasks of bromthymol blue until they became acidic. A sprig of Elodea was placed in each flask. One flask was left in green light for 24 hours, and the other flask was left in red light for 24 hours. Not change occurred in the flask left in green light. The bromthymol blue in the flask that was left in the red light turned back to blue. Explain the results of the following experiment: A sprig of Elodea was placed into each of two flasks containing bromthymol blue. One flask was left in the dark for 24 hours; the other was left in the light for 24 hours. The bromthymol blue in the flask in the dark turned yellow. The other remained blue. Based on your understanding of the chemistry of photosynthesis, why do plants need animals in order to survive? NOTES FOR TEACHER: To make the bromthymol blue solution, if you only have powdered stock on hand, measure out 0.002 grams of powder and all to 500 mL of distilled water. When you add the drops of bromthymol blue to the test tubes containing Elodea, the water should turn a blue tint. When the students blow through the straw into the test tube containing the Elodea and bromthymol blue solution it make take between 30-60 seconds for the solution to become clear. They must do this CAREFULLY as you can splash solution everywhere, DO NOT DRINK SOLUTION! As photosynthesis occurs and O2 is added back into the test tube containing the Elodea and bromthymol blue solution it will change back to the blueish tint, the test tubes placed in the dark should remain clear, or the faint yellow green. ( No light, no photosynthesis, no O2 bubbles) I would have the students read and prepare for this lab the day before, or as homework. 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