ࡱ> _ \bjbjzz 1B\B\T%jjjjj~~~8\T~'Qff"cccPPPPPPP$RTPj*cc**PjjP*jjP*PIXN R.&L(PP0'QNL[Ul[UPNN[UjNcvT-DqcccPPdXccc'Q****[Uccccccccc> 0:  Vanderbilt Student Volunteers for Science Water Quality Fall 2006 Goal: To test sources of drinking water for chlorine content and pH. To test swimming pool water and compare chlorine content and pH to that of drinking water. Lesson Outline I. Introduction Discuss the sources of drinking water surface water (rivers, lakes) and well water). Nashville gets its drinking water from the Cumberland River. The water treatment plant removes contaminants and adds chlorine to kill pathogenic organisms. II. Demonstration - Parts per Million Visualizing ppm with Salt Samples and Sheet of Million Dots Use the different samples of salt to explain ppm. Use the sheet of dots to demonstrate Parts per Million (ppm). Use the red food dye, a 12-well plate, and dilutions to demonstrate ppm. III. Testing Water Samples Students use a Pool and Spa Water Quality Test Kit to measure chlorine and pH on several water samples. You will need to prepare swimming pool water using Clorox Directions are given in the lesson for preparing this sample. Other samples will include water from a drinking fountain at the school, tap water from a sink in the classroom. You can also ask students to bring water samples from home be sure they are label the source. IV. Discussion of Students Results Write sample data from the various water sample tests on the board and compare the levels of chlorine and the pH values. Emphasize the higher level of chlorine in swimming pool water. Review ppm. Materials 1 ziploc bag containing 1 12-well plate, 1 container of red food dye, 10 plastic pipettes, 1 small spoon 1 ziploc bag containing salt samples for ppm demonstration jar of 1 million salt crystals with 1 orange crystal for 1 ppm matrix dot sheet for ppm demonstration 1 ziploc bag containing 1 small container of Clorox 1 10 mL graduated cylinder 1 25 mL graduate cylinder 2 plastic pipettes 1 2 oz cup for making Clorox sample 1 small spoon 1 liter container marked swimming pool water filled to the mark with tap water 8 3.5 oz cups marked swimming pool water with a line at the 30 mL level. 8 3.5 oz cups marked drinking fountain water with a line at the 30 mL level 8 3.5 oz cups marked tap water with a line at the 30 mL level 1 250 mL container marked drinking fountain water 1 250 mL container marked tap water 10 Pool and Spa Water Quality Test Kits 32 plastic pipettes (four per group) PREPARATION: While one of the team leads the discussion and does the ppm demonstrations, other members need to do the following: Make a sample that simulates swimming pool water. Measure out 5 mL of liquid chlorine bleach (Clorox) and place it in the small clear cup provided. Then add 15 mL of tap water. Use small spoon to stir well. Then add 20 drops of this dilute solution to the 1 liter container marked swimming pool water. It already contains 1 L of water. Put on the screw cap and shake well to mix the solution. This is about the same concentration of chlorine as you find in a swimming pool or hot tub and about ten times the amount found in drinking water. Pour 30 mL of this swimming pool water into 8 3.5 oz cups labeled swimming pool water Fill the container marked drinking fountain water from a drinking fountain in the school. Pour 30 mL of this water sample into 8 3.5 oz cups labeled drinking fountain water. Fill the container marked tap water from a water faucet in the classroom or from a water faucet in one of the restrooms. Pour 30 mL of this water sample into 8 3.5 oz cups labeled drinking fountain water. I. Introduction It is very important that we consume water that is not contaminated because water is our most important resource, and it can easily affect our health very quickly. Many different components can be considered as a contaminant, such as pollutants and waste, viruses, dangerous heavy metals like lead and mercury, strange minerals, and even ionic concentrations that are too high. As far as tap water is concerned, the city of Nashville tests its water frequently for such contaminants. The city adds chlorine to water to kill germs (pathogenic organisms) and fluoride ion to aid in dental health. However, very high concentrations of either chemical can have adverse effects. If someone draws their water from a well, other components such as nitrates, carbonates, copper, and iron might be present in an analysis. So, basically, it is crucial to know and understand what exactly is in the water that you drink. Even the cleanliness of pool and spa water is necessary. People add enough chlorine to pools to make sure the water is safe for swimmers. After contaminants are considered, water quality investigators look to pH, the acidity or alkalinity, of water because drinking water should be very close to neutral, 7, while pool water should register a pH that is slightly basic, between 7.2 and 7.6. Water can become harmful to humans and wildlife if the pH gets too high or too low. For example, most fish cannot survive in environments of a pH below 5. Any type of water, drinking or swimming, is constantly under surveillance for good reason because without healthy water to consume and to use recreationally, the health of humans can decline very fast. It is true that most water contains some contaminants mentioned above (pools and Nashville tap water both have chlorine). Obviously, in the proper amounts, these contaminants are necessary, but what are the proper amounts? How much is too much? To understand the limitations of the EPA, Environmental Protection Agency, you must understand a few of their terms. Parts per million: ppm: 1mg of contaminant in 1L of water Parts per billion: ppb: 1mg of contaminant in 1000L of water Maximum Contaminant Level: MCL: the highest amount of contaminant that is allowed in the water at any given time National Primary Drinking Water Regulations: NPDWRs: the legally enforceable standards applied to drinking water Here is the MCL value for chlorine and the permitted pH range. The associated heath risks when these values are exceeded are also given. MCLHealth RiskChlorine4.0 ppmEye/nose irritation, stomach discomfortpH6.5-8.5Stomach irritation Discussion of Parts Per Million Start with a discussion based on visual experiences that will help the students understand parts per million (ppm). Some examples that can be used are given below. 1 ppm is 1 minute in 2 years. 1 ppm is 1 inch in 16 miles. 1 ppm is 1 penny in $10,000 A. Visualizing ppm with Salt Sample and millions of dots Use the salt samples and the sheet of a million dots in the kit to help student better understand ppm. Salt Samples There are vials containing 1, 10, 100, 1000, 10,000, 100,000, and 1,000,000 salt crystals. Let students look at these. Another jar contains 1,000,000 salt crystals and one bright orange crystal of potassium dichromate. Challenge the students to find the orange crystal. This one crystal represents 1 ppm since it is one orange crystal in one million salt crystals. 2. Unfold the newspaper and show the sheet of a million dot. One dot is 1 ppm. B. Red Food Dye Dilutions Demonstration of ppm and ppb Use the 12-well plate and red food dye and carry out the following activity taken from CEPUP, Chemicals and Society, pp. 5, 6. (See Reference) This works best if you place the 12-well plate on an overhead projector. Tell them the following: I am going to put 10 drops of the 10% red food dye into well 1 (first well on top row.) Ask: What is the concentration of food coloring in this solution? Answer: 10% means 10 parts food coloring per 100 parts solution or 1 part in 10. I will now put 4 drops of well 1 to well 2 and add 36 drops of water. Ask: What is the resulting concentration? Answer: 4 parts of food coloring plus 36 parts of water results in 4 parts in 40 or 1 part in 10 more dilute than well 1 which means well 2 is 1 part food coloring per 100 parts of solution. Stir the solution in well 2 with a small spoon. Then use a clean, dry pipette to transfer 4 drops of well 2 to well 3 and add 36 drops of water. This again changes the concentration by one-tenth. Ask: What is the food coloring concentration in well 3? Answer: 1/10 of 1 part in 100 is 1 part in 1,000. Repeat this procedure of mixing the solution in wells, adding 4 drops from the last well to the next well, for wells 4,5,6,7,8,9. Wipe the spoon off with a paper towel between stirrings. Well 4: 1 part food coloring in 10,000 parts of solution Well 5: 1 part food coloring in 100,000 parts of solution Well 6: 1 part food coloring in 1,000,000 parts of solution = 1 ppm Well 7: 1 part food coloring in 10,000,000 parts of solution Well 8: 1 part food coloring in 100,000,000 parts of solution Well 9: 1 part food coloring in 1,000,000,000 parts of solution = 1ppb Ask the following questions: In which cup do we first observe no color evidence that food coloring is present? This usually occurs in the sixth cup which is 1ppm food coloring. 2. Since we cant see any color, does that mean there is no food coloring present? No 3. How do we know there is food coloring present? We continued to take 4 drops from the previous well for each succeeding well so every well contains some food coloring, even if we cant see it. III. Testing Methods for Water Quality Chlorine and pH For this lesson, the students will use of a Pool and Spa Water Quality Test Kit to test for chlorine and pH. The range for chlorine is 0.0ppm to 3.0ppm, which is indicated by an array of yellow colors. Clear or light yellow results mean that chlorine is very low or at a safe level whereas darker yellows and orange results mean that chlorine exists at a possibly unsafe level. The range for pH is 6.8 to 8.2. IV. Technique Give each group one of the Pool and Spa Water Quality Kits, 4 plastic pipettes, a waste container, and a cup of each of the samples. Tell them to test each of the water samples for chlorine in the following order: tap water, drinking fountain water, swimming pool water. They should place one of the pipettes in each of the water samples. A fourth pipette is provided in case you have another water sample for students to test. After each test, they can empty the water sample into their waste container. Emphasize that good testing procedures require that after the vial is emptied, the sample vial should be rinsed with a small amount of the next sample being tested before filling to the line. This is done by first emptying out the plastic pipette in the waste container, and filling the plastic pipette with some of the next sample, emptying this into the vial, and then emptying the vial. After rinsing, they can use the pipette to fill the vial to the mark. NOTE: After the students start the tests, members of the teaching team should use one of the extra Pool and Spa Kits to test for chlorine in tap water and another kit to test for chlorine in swimming pool water. Have these available to help students see the difference in intensity of colors. V. General Procedure Give each group one of the Pool and Spa Water Quality Kits, 4 plastic pipettes, a waste container, and a cup of each of the samples. A. Test each of the water samples for chlorine in the following order: tap water, drinking fountain water, swimming pool water. B. Place one of the pipettes in each of the water samples. Make sure each sample has its own pipette and dont use them for any other sample. A fourth pipette is provided in case you have another water sample to test. C. After each test, empty the water samples into a waste container. D. Good testing procedures require that after the testing vials are emptied, they should be rinsed with a small amount of the next sample being tested before filling to the line. Use the pipette from the next sample to rinse and fill the vial to the mark. .VI. Chlorine and pH Testing 1A. Tap water sample Chlorine Test a. Using the left vial marked Cl, fill the vial to the line with sample tap water using the plastic pipette in your tap water sample. b. Add 5 drops of OTO solution. Put cap on vial and invert several times to mix the solution. The color of the solution turns yellow if chlorine is present. c. Compare the color to the color chart next to the chlorine vial. Record the number which gives the best color match. This is the amount of chlorine in parts per million (ppm) which is present in the sample. 1B. Tap water sample pH Test Using the right vial marked pH, fill the vial to the line with sample tap water using the plastic pipette. Add 5 drops of phenol red solution. Put cap on vial and invert several times to mix the solution. Compare the color to the color chart next to the pH vial. Record the number that gives the best color match. This is the pH of the sample. Empty both vials of test kit into a waste container. 2A. Drinking fountain sample Chlorine Test a. Use the pipette in the drinking fountain sample to rinse both testing vials with the drinking fountain water by filling the vial about half-full and emptying this into your waste container. b. Use the pipette to fill the left vial to the line with the sample drinking fountain water. c. Add 5 drops of OTO solution. Put the cap on vial and invert several times to mix the solution. d. Compare the color to the color chart next to the chlorine vial. Record the number which gives the best color match. 2B. Drinking fountain water sample pH Test a. Using the right vial marked pH, use the pipette to fill the vial to the line with sample drinking fountain water. b. Add 5 drops of phenol red solution. Put cap on vial and invert several times to mix the solution. c. Compare the color to the color chart next to the pH vial. Record the number that gives the best color match. This is the pH of the sample. Empty both vials of test kit into your waste container. 3A. Swimming pool sample Chlorine Test a. Use the pipette in the swimming pool sample to rinse the test vials with the swimming pool water by filling the vial about half-full and emptying this into your waste container. b. Use the pipette to fill the left vial to the line with the sample swimming pool water. c. Add 5 drops of OTO solution. Put the cap on vial and invert several times to mix the solution. d. Compare the color to the color chart next to the chlorine vial. Record the number which gives the best color match. 3B. Swimming pool water sample pH Test a. Using the right vial marked pH, use the pipette to fill the vial to the line with sample swimming pool water. b. Add 5 drops of phenol red solution. Put cap on vial and invert several times to mix the solution. Compare the color to the color chart next to the pH vial. Record the number that gives the best color match. This is the pH of the sample. Empty both vials of your test kit into your waste container. Follow the same procedure as before for testing chlorine and pH in other samples brought for testing. Remember to rinse the vials with a small amount of sample before filling to the mark. Record the number which gives the best color match for the chlorine and pH tests. VII. Discussion of Students Results Questions Which water sample had the most chlorine? highest pH? Were there any samples that did not have chlorine present? If so, why? VIII. Conclusions Discussion: Nashville water is treated with chlorine to kill bacteria. A residual level of chlorine is required to keep the water safe from the time it leaves the water treatment plant until it reaches the home and is used. The residual amount varies from 0.5 ppm to 1 ppm. A higher level of residual chlorine is necessary during the summer months since bacteria grow faster at higher temperatures. Swimming pool and hot tub water have higher levels of chlorine, usually 3 ppm or above. Students should have observed that swimming pool water had the highest content of chlorine. Drinking fountain water may turn out to be the same or slightly higher than tap water. If other samples were used, include these in the comparison. Reference: Chemicals in Society, a lesson by H.D. Their and J.E. Davis, Jr., published by the Chemical Education for Public Understanding Program (CEPUP), Lawrence Hall of Science, University of California at Berkeley. Lesson written and tested by: Dr. Mel Joesten, VSVS Faculty Adviser Frank Merendino, NSF Undergraduate Teaching Fellow August, 2006 Data Sheet Quality of Drinking Water and Swimming Pool Water Name __________________________________ Federal Regulation Limits MCLHealth RiskChlorine4.0 ppmEye/nose irritation, stomach discomfortpH6.5-8.5Stomach irritation A. tap water sample AnalysisColorAmount or pHEPA Approved?Chlorine ppmpH pH B. drinking fountain sample AnalysisColorAmount (ppm)EPA Approved?Chlorine  ppmpH pH C. swimming pool or spa sample AnalysisColorAmount (ppm)EPA Approved?Chlorine ppmpH pH D. ____________________ sample AnalysisColorAmount (ppm)EPA Approved?Chlorine ppmpH pH Questions 1. Which method was the most accurate for detecting pH? Why? 2. Which water sample had the most chlorine? highest pH? 3. If well water was used as a sample, how did it differ from the fountain water? 4. Were there any samples that did not have chlorine present? If so, why? INSTRUCTION SHEET WATER QUALITY General Procedure A. Test each of the water samples for chlorine in the following order: tap water, drinking fountain water, swimming pool water. B. Place one of the pipettes in each of the water samples. Make sure each sample has its own pipette and dont use them for any other sample. A fourth pipette is provided in case you have another water sample to test. C. After each test, empty the water samples into a waste container. D. Good testing procedures require that after the testing vials are emptied, they should be rinsed with a small amount of the next sample being tested before filling to the line. Use the pipette from the next sample to rinse and fill the vial to the mark. .II. Chlorine and pH Testing 1A. Tap water sample Chlorine Test a. Using the left vial marked Cl, fill the vial to the line with sample tap water using the plastic pipette in your tap water sample. b. Add 5 drops of OTO solution. Put cap on vial and invert several times to mix the solution. The color of the solution turns yellow if chlorine is present. c. Compare the color to the color chart next to the chlorine vial. Record the number which gives the best color match. This is the amount of chlorine in parts per million (ppm) which is present in the sample. 1B. Tap water sample pH Test Using the right vial marked pH, fill the vial to the line with sample tap water using the plastic pipette. Add 5 drops of phenol red solution. Put cap on vial and invert several times to mix the solution. Compare the color to the color chart next to the pH vial. Record the number that gives the best color match. This is the pH of the sample. Empty both vials of test kit into a waste container. 2A. Drinking fountain sample Chlorine Test Use the pipette in the drinking fountain sample to rinse the vials with the drinking fountain water by filling the vial about half-full and emptying this into your waste container. Use the pipette to fill the left vial to the line with the sample drinking fountain water. Add 5 drops of OTO solution. Put the cap on vial and invert several times to mix the solution. Compare the color to the color chart next to the chlorine vial. Record the number which gives the best color match. 2B. Drinking fountain water sample pH Test a. Using the right vial marked pH, use the pipette to fill the vial to the line with sample drinking fountain water. b. Add 5 drops of phenol red solution. Put cap on vial and invert several times to mix the solution. Compare the color to the color chart next to the pH vial. Record the number that gives the best color match. This is the pH of the sample. Empty both vials of test kit into your waste container. 3A. Swimming pool sample Chlorine Test a. Use the pipette in the swimming pool sample to rinse the vials with the swimming pool water by filling the vial about half-full and emptying this into your waste container. b. Use the pipette to fill the left vial to the line with the sample swimming pool water. c. Add 5 drops of OTO solution. Put the cap on vial and invert several times to mix the solution. d. Compare the color to the color chart next to the chlorine vial. Record the number which gives the best color match. 3B. Swimming pool water sample pH Test a. Using the right vial marked pH, use the pipette to fill the vial to the line with sample swimming pool water. b. Add 5 drops of phenol red solution. Put cap on vial and invert several times to mix the solution. Compare the color to the color chart next to the pH vial. Record the number that gives the best color match. This is the pH of the sample. Empty both vials of your test kit into your waste container. Follow the same procedure as before for testing chlorine and pH in other samples brought for testing. Remember to rinse the vials with a small amount of sample before filling to the mark. Record the number which gives the best color match for the chlorine and pH tests.     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