ࡱ> q` 4bjbjqPqP *:: ,HHHHHHH\D/D/D/8|/D/\(>&P1P1(x1x1x1S2S2S2=======$N?hAb=H3S2S233=HHx1x1=/7/7/73Hx1Hx1=/73=/7/7HH/7x1D1 #yRلD/5/77=0(>/7B5B/7BH/7S2"u2/722S2S2S2==6XS2S2S2(>3333\\\&`,\\\`,\\\HHHHHH BLOOD COMPONENT COMPATIBILITY REQUIREMENTSPRIVATE  PURPOSE To select blood components immunologically compatible with individual recipients. POLICIES FOR ROUTINE SELECTION OF RED CELLS, WHOLE BLOOD AND GRANULOCYTES 1. Routine pretransfusion testing for RBC containing components includes: A. ABO and RHo(D) typing, including a test for weak D phenotypes (Du), performed at least twice, B. Antibody detection test (antibody screen) using a twocell panel (no autocontrol),and monospecific antihuman-IgG serum. C. If the antibody screen is negative and ABO discrepancy is absent, major crossmatch between recipient and donor at immediate spin only using 2 drops of patient serum in tubes, If the antibody screen is positive or if there is a history of a clinically significant blood group antibody, major crossmatch with 4 drops patient serum, and monospecific antihuman IgG serum after resolution of the serologic problem. NOTE: Patients with a history of a warm autoantibody and NO clinically significant alloantibody whose antibody screen is currently negative don't require AHG crossmatches. NOTE: Rouleaux is resolved by the saline displacement technique, and an AHG crossmatch is NOT required. 2. When serologic problems are encountered, steps to resolve them should be initiated. If transfusion is urgent, a supervisor or blood bank physician should be consulted. 3. ABO specific blood components are issued whenever available with the exception of perinatal transfusions. The policies below govern acceptable alternatives when such components are not available or when the patient has requested directed donations. 4. Rhnegative patients must receive Rhnegative blood components whenever available. Rhpositive patients may receive Rhpositive or negative units. (These two statements define "Rhspecific" as used below). Individuals with weak D phenotypes (Du) are considered Rh-positive. When Rhpositive RBCs or whole blood must be issued to Rhnegative recipients consult a supervisor and/or a blood bank physician. (NOTE: HANDLE EMERGENCIES FIRST. Then notify the physician/supervisor.) These individuals may require that the physician sign an Emergency Blood Request form; also, use of RhIG may be indicated. 5. Whole Blood must be ABOspecific. 6. RBC-containing components must be ABOcompatible with the recipient's serum and Rhspecific (see #4 above). When ABOspecific RBCs are not available or are available in insufficient quantities select the next best choice according to the following chart: PRIVATE Recipient's GroupAlternate ABO Alternate Rh1st Choice2nd ChoiceMen, Women > 50Women <50 y.o.O posNoneNoneNegNegA posONoneNegNegB posONoneNegNegAB posA or BONegNegO negNoneNonePos*None*A negONonePos*None*B negONonePos*None*AB negA or BOPos*None* * Rh negative men and women over the age of 50 yrs may receive Rh positive RBCs or whole blood in an emergency (see #4 above); Rh negative women under 50 should only get Rh Positive blood if they are surgically sterile (hysterectomy, etc.) or in the most dire emergency. 7. ABOnonspecific transfusions may be discontinued and the patient switched back to RBCs of their own group without special testing if 7 or less units of RBCs have been transfused. If more than 7 units of non-specific RBCs, or blood components containing more than 500 cc of plasma incompatible with the recipient, have been transfused in the previous 24 hours, the patient should continue to receive RBCs compatible with the donor antibody until: A. transfused anti-A and/or anti-B is not detectable with reagent group A and B cells in the saline and AHG phases, AND B. the crossmatch is compatible at all phases. If these conditions are met the recipient can be switched back to his or her own blood group. POLICIES FOR ROUTINE SELECTION OF PLATELETS 1. Pretransfusion testing for platelets includes ABO and RH blood grouping and an antibody screen. These tests need not be repeated if blood typing and an antibody screen has been performed on at least 2 occasions if one of the specimens was drawn in the previous year, or if it was done during the current hospitalization (even if never performed previously). Issue of platelets should not be delayed for resolution of a serologic problem detected by these tests. 2. Judgement is required in selection of the blood group of platelet transfusions. The following principles should be applied to this decision: A. An effort must be made to conserve platelet units that might outdate. B. Because platelets have ABO antigens, chronic platelet users may become refractory to repeated transfusion of platelets having ABO antigens against which the patient has antibody. An acute need for platelets overrides this concern in chronic users, and the concern does not apply to one-time users such as surgery patients. Recipients GroupAlternate ABO for Platelets (See also Policy 2C & 2D)1st Choice2nd Choice3rd ChoiceO posA or BABA posABOBB posABOAAB posABO C. Transfusion of large numbers of platelet units containing ABO antibody directed against the recipient's RBCs, may cause a positive DAT, and occasionally hemolysis. If more than 10 such units of Platelets (from whole blood) or 2 units of single donor platelets in a single day are to be issued consult a supervisor or blood bank physician. D. RBCs invariably contaminate platelet units so units from Rh-positive donors can immunize Rh-negative recipients. Notify a blood bank physician if Rh-positive units must be issued to an Rh-negative girl or woman of childbearing age (use of RhIG may be indicated). 3. Platelet units (single donor or pools of concentrates) contaminated with more than 2 cc of red cells (units with an obvious red color) must be crossmatch-compatible with the recipient's serum. Use RBCs from the donor pilot tube for crossmatching (consult a supervisor or blood bank physician if unavailable.) 4. Platelets collected from donors who have ingested aspirin must not serve as the sole source of platelet preparations for a recipient. If a single unit of Platelets (prepared from whole blood is needed for a recipient (usually a neonate), an aspirin free platelet concentrate must be used. POLICIES FOR ROUTINE SELECTION OF PLASMA AND CRYOPRECIPITATE 1. Pretransfusion testing for FFP or cryoprecipitate includes ABO and RH blood grouping and an antibody screen. These tests need not be repeated if blood typing and an antibody screen has been performed on at least 2 occasions, and if one of the specimens was drawn in the previous year, or if typing was done during the current hospitalization (even if never performed previously). Issue of plasma components should not be delayed for resolution of a serologic problem detected by these tests. 2. FFP must be ABOcompatible with the recipient's red cells (see table below). If compatible cryoprecipitate is not available, other groups may be substituted. FFP and cryo need NOT be Rh-specific. Recipients GroupAlternate ABO for FFP1st Choice2nd ChoiceO posA or BABA posABNoneB posABNoneAB posNoneNone POLICIES FOR SELECTION OF RED CELLS FOR PATIENTS WITH BLOOD GROUP ANTIBODIES Patients with clinically significant blood group antibodies in their serum or a history of previously identified clinically significant blood group antibodies will receive RBCs which are free of the corresponding antigen by testing with reagent antisera when available, as well as compatible through the AHG phase of the crossmatch, subject to the following clarifications and modifications: 1. Patients with a history of cold-reactive autoantibodies or antiP1, M, Le(a), Le(b) or -A1 antibodies, NOT currently detectable by antibody screen (or reverse typing in the case of anti-A1) should be issued IS crossmatch compatible blood (i.e. not tested for antigen with reagent antisera). 2. Patients with detectable antiP1, M, or -A1 should receive blood which is antigen negative by reagent antisera ONLY if the antibody is reactive at 37oC or the AHG phase. Otherwise issue of RBCs compatible at 37oC and AHG blood is sufficient. (Note: incompatibility at IS can be ignored. Use a pre-warmed crossmatch procedure if carryover has been observed.) 3. Patients with detectable antiLe(a) or antiLe(b) should be issued RBCs compatible at 37oC and AHG. (Note: RBCs need not be tested for Le antigens with reagent antisera, and incompatibility at IS can be ignored.) 4. Patients with anti-D and documented administration of RhIG in the previous 3 months should be issued (Rh negative) RBCs, compatible by IS-crossmatch unless other antibodies are present as well. 5. Patients with other clinically IN-significant alloantibodies causing incompatible crossmatches at the 37o C or AHG phases (e.g. HTLAs, anti-Bg) should receive AHG crossmatch compatible RBCs if possible. In addition to identifying the antibody specificity, the workup in such cases should focus on ruling out other clinically significant antibodies. 6. Patients with cold autoantibodies causing incompatible crossmatches at the 37o C or AHG phases should receive RBCs compatible at 37oC and AHG phases using autoabsorbed patient sera or by the prewarmed technique. 7. Patients with warm autoantibodies should receive AHG crossmatch compatible blood using warm autoabsorbed patient sera if possible, or, for patients transfused in the past 3 months, serum that is allo-adsorbed with appropriately selected RBCs. Consult with a supervisor for any further requirements (e.g. antigen negative units). At a blood bank physician's discretion, the recipient's physician may need to sign an "Emergency Blood Request" form if crossmatches with raw serum would be incompatible (i.e. blood bank physician should be consulted). 8. For patients with antibodies, repeat pretransfusion testing is performed with a selected cell panel (see Investigation of a Positive Antibody Detection Test). When such testing shows evidence of new reactivity (e.g. stronger reactions, additional screening cells positive, etc.) they must be reevaluated. 9. In some cases of massive transfusion it may be appropriate to abbreviate testing after a large number of units have been transfused. Consult a supervisor if a patient with a blood group antibody has received 10 units or more of RBCs. POLICY FOR SELECTION OF RBCS FOR PATIENT WITH SICKLE CELL DISEASE OR THALASSEMIA Patients with sickle cell disease (SS, SC, S(thalassemia, others) or thalassemia should receive RBCs lacking Rh and Kell antigens (D, C, c, E, e, K) that the patient lacks. For example a patient with the phenotype DCcek should receive RBCs with the phenotype DCcek, Dcek, or DCek) but not DCcEek etc. This rule applies to routine transfusions;in hemorrhagic or other emergencies RBC selection should follow the same rules as for other patients. 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