ࡱ> #%"? L jbjb }}Ll  &$$$$$$$$, $$$$$8$$$888$ $$8$8Z8 ֺ. 88Titering Virus-Protocol Plate 2x106 cells in Lux plates in total of 3ml (e.g. 1ml Sf9 at 2x106 and 2ml medium) Allow to attach, then aspirate supernatant: For each virus make a 10-5, 10-6, 10-7 dilution in Graces complete e.g. make 1:100 dilution (5(l in 5 ml), then dilute 1:100 (50(l in 5ml)(10-5 dilute 1:10 (500(l in 5ml)(10-6 dilute 1:10 (500(l in 5ml)(10-7 Pipet 1ml of each dilution onto a separate dish of cells. Incubate for 1h at 280C, agitating by rocking every 10 min, then aspirate virus. Meanwhile, pre-warm 40ml medium to 370C. Melt 5% LMP-agarose, mix well. Allow to cool to app, 28.370C, then carefully pipet 4ml per dish onto cells. Incubate at 280C for 5-6 days. On day 5 or 6 make 1% LPM-agarose/graces as before, but add to it 1ml 1% Trypman blue, mix and add 2ml to each plate. In 1 or 2 days, count plaques. #$^`!"+,.0BCLMOQcdmnpr&'deL jCJ jmCJCJH*CJ(pq1Rstu- L $`a$$a$pq1Rstu- L / =!"#$% i4@4NormalCJOJPJQJmH <A@<Default Paragraph Font,>`,Title$a$5CJ L   zLL L L NNSloan Kettering InstituteOMacintosh HD:Temporary Items:501:Temporary Items:AutoRecovery save of Document1Sloan Kettering InstituteEMacintosh HD:Users:nikolov_g4:Desktop:Doroty:Protocols:Titering VirusSloan Kettering InstituteEMacintosh HD:Users:nikolov_g4:Desktop:Doroty:Protocols:Titering Virus@KKzK6(YL @GTimes New Roman5Symbol3 Arial;Wingdings3Times h u u~0dL"Titering Virus-ProtocolSloan Kettering InstituteSloan Kettering Institute Oh+'0 , H T `lt|'Titering Virus-ProtocoliteSloan Kettering Institute0loaNormaleSloan Kettering Institute02oaMicrosoft Word 9.0t@q@2@M# ՜.+,0 hp  'MSKCCg Titering Virus-Protocol Title  !$Root Entry F &1Table WordDocumentSummaryInformation(DocumentSummaryInformation8CompObjX FMicrosoft Word DocumentNB6WWord.Document.8