ࡱ> )      { |bjbjzz L!u|PP**,,,D,,,J-f5,V" =Xd8Y8YYmTDUFUFUFUFUFUFU$[]^jU,֙#~֙֙jU**8YY{$V2 ֙B*V8Yp,YDU ֙DU " d,-Y`s- 0%v0UMV<V%_ޜ _--_,8Ae>, Ϙ$eeejUjUeeeV֙֙֙֙_eeeeeeeeeP p):  December 2012 Teacher's Guide Table of Contents  TOC \o "1-3" \h \z \u  HYPERLINK \l "_Toc348534899" About the Guide  PAGEREF _Toc348534899 \h 3  HYPERLINK \l "_Toc348534900" Student Questions (from the articles)  PAGEREF _Toc348534900 \h 4  HYPERLINK \l "_Toc348534901" Answers to Student Questions (from the articles)  PAGEREF _Toc348534901 \h 6  HYPERLINK \l "_Toc348534902" ChemMatters Puzzle: Chemical Syllabism  PAGEREF _Toc348534902 \h 10  HYPERLINK \l "_Toc348534903" Answers to the ChemMatters Puzzle  PAGEREF _Toc348534903 \h 11  HYPERLINK \l "_Toc348534904" NSES Correlation  PAGEREF _Toc348534904 \h 12  HYPERLINK \l "_Toc348534905" Anticipation Guides  PAGEREF _Toc348534905 \h 13  HYPERLINK \l "_Toc348534906" The Big Reveal: Whats Behind Nutrition Labels  PAGEREF _Toc348534906 \h 14  HYPERLINK \l "_Toc348534907" Two Is Better than One  PAGEREF _Toc348534907 \h 15  HYPERLINK \l "_Toc348534908" Whats that Smell?  PAGEREF _Toc348534908 \h 16  HYPERLINK \l "_Toc348534909" Mascara: That Lush Look You Love!  PAGEREF _Toc348534909 \h 17  HYPERLINK \l "_Toc348534910" Dirty Business: Laundry Comes Clean with Chemistry  PAGEREF _Toc348534910 \h 18  HYPERLINK \l "_Toc348534911" Reading Strategies  PAGEREF _Toc348534911 \h 19  HYPERLINK \l "_Toc348534912" The Big Reveal: Whats Behind Nutrition Labels  PAGEREF _Toc348534912 \h 20  HYPERLINK \l "_Toc348534913" Two Is Better than One  PAGEREF _Toc348534913 \h 21  HYPERLINK \l "_Toc348534914" Whats that Smell?  PAGEREF _Toc348534914 \h 22  HYPERLINK \l "_Toc348534915" Mascara: That Lush Look You Love!  PAGEREF _Toc348534915 \h 23  HYPERLINK \l "_Toc348534916" Dirty Business: Laundry Comes Clean with Chemistry  PAGEREF _Toc348534916 \h 24  HYPERLINK \l "_Toc348534917" The Big Reveal: Whats Behind Nutrition Labels  PAGEREF _Toc348534917 \h 25  HYPERLINK \l "_Toc348534918" Background Information (teacher information)  PAGEREF _Toc348534918 \h 25  HYPERLINK \l "_Toc348534919" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc348534919 \h 35  HYPERLINK \l "_Toc348534920" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc348534920 \h 36  HYPERLINK \l "_Toc348534921" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc348534921 \h 36  HYPERLINK \l "_Toc348534922" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc348534922 \h 36  HYPERLINK \l "_Toc348534923" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc348534923 \h 37  HYPERLINK \l "_Toc348534924" References (non-Web-based information sources)  PAGEREF _Toc348534924 \h 37  HYPERLINK \l "_Toc348534925" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc348534925 \h 39  HYPERLINK \l "_Toc348534926" More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers)  PAGEREF _Toc348534926 \h 41  HYPERLINK \l "_Toc348534927" Two Is Better than One  PAGEREF _Toc348534927 \h 42  HYPERLINK \l "_Toc348534928" Background Information (teacher information)  PAGEREF _Toc348534928 \h 42  HYPERLINK \l "_Toc348534929" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc348534929 \h 52  HYPERLINK \l "_Toc348534930" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc348534930 \h 52  HYPERLINK \l "_Toc348534931" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc348534931 \h 52  HYPERLINK \l "_Toc348534932" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc348534932 \h 53  HYPERLINK \l "_Toc348534933" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc348534933 \h 53  HYPERLINK \l "_Toc348534934" References (non-Web-based information sources)  PAGEREF _Toc348534934 \h 54  HYPERLINK \l "_Toc348534935" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc348534935 \h 54  HYPERLINK \l "_Toc348534936" More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers)  PAGEREF _Toc348534936 \h 56  HYPERLINK \l "_Toc348534937" Whats That Smell?  PAGEREF _Toc348534937 \h 57  HYPERLINK \l "_Toc348534938" Background Information (teacher information)  PAGEREF _Toc348534938 \h 57  HYPERLINK \l "_Toc348534939" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc348534939 \h 66  HYPERLINK \l "_Toc348534940" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc348534940 \h 67  HYPERLINK \l "_Toc348534941" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc348534941 \h 67  HYPERLINK \l "_Toc348534942" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc348534942 \h 69  HYPERLINK \l "_Toc348534943" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc348534943 \h 69  HYPERLINK \l "_Toc348534944" References (non-Web-based information sources)  PAGEREF _Toc348534944 \h 70  HYPERLINK \l "_Toc348534945" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc348534945 \h 71  HYPERLINK \l "_Toc348534946" Mascara: That Lush Look You Love!  PAGEREF _Toc348534946 \h 74  HYPERLINK \l "_Toc348534947" Background Information (teacher information)  PAGEREF _Toc348534947 \h 74  HYPERLINK \l "_Toc348534948" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc348534948 \h 81  HYPERLINK \l "_Toc348534949" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc348534949 \h 82  HYPERLINK \l "_Toc348534950" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc348534950 \h 82  HYPERLINK \l "_Toc348534951" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc348534951 \h 82  HYPERLINK \l "_Toc348534952" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc348534952 \h 83  HYPERLINK \l "_Toc348534953" References (non-Web-based information sources)  PAGEREF _Toc348534953 \h 84  HYPERLINK \l "_Toc348534954" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc348534954 \h 84  HYPERLINK \l "_Toc348534955" Dirty Business: Laundry Comes Clean with Chemistry  PAGEREF _Toc348534955 \h 86  HYPERLINK \l "_Toc348534956" Background Information (teacher information)  PAGEREF _Toc348534956 \h 86  HYPERLINK \l "_Toc348534957" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc348534957 \h 101  HYPERLINK \l "_Toc348534958" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc348534958 \h 102  HYPERLINK \l "_Toc348534959" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc348534959 \h 103  HYPERLINK \l "_Toc348534960" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc348534960 \h 103  HYPERLINK \l "_Toc348534961" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc348534961 \h 106  HYPERLINK \l "_Toc348534962" References (non-Web-based information sources)  PAGEREF _Toc348534962 \h 107  HYPERLINK \l "_Toc348534963" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc348534963 \h 108  HYPERLINK \l "_Toc348534964" More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers)  PAGEREF _Toc348534964 \h 111  About the Guide Teachers Guide editors William Bleam, Donald McKinney, Ronald Tempest, and Erica K. Jacobsen created the Teachers Guide article material. E-mail:  HYPERLINK "mailto:bbleam@verizon.net" bbleam@verizon.net Susan Cooper prepared the national science education content, anticipation guides, and reading guides. David Olney created the puzzle. E-mail:  HYPERLINK "mailto:djolney@verizon.net" djolney@verizon.net Patrice Pages, ChemMatters editor, coordinated production and prepared the Microsoft Word and PDF versions of the Teachers Guide. E-mail:  HYPERLINK "mailto:chemmatters@acs.org" chemmatters@acs.org Articles from past issues of ChemMatters can be accessed from a CD that is available from the American Chemical Society for $30. The CD contains all ChemMatters issues from February 1983 to April 2008. The ChemMatters CD includes an Index that covers all issues from February 1983 to April 2008. The ChemMatters CD can be purchased by calling 1-800-227-5558. Purchase information can be found online at  HYPERLINK "http://chemistry.org/chemmatters/cd3.html" www.acs.org/chemmatters Student Questions (from the articles) The Big Reveal: Whats Behind Nutrition Labels What information does a nutrition label on food typically contain? What is the definition of a Calorie? What is the difference between a nutritional Calorie (uppercase C) and a calorie (lowercase c)? How many Calories per gram are found in carbohydrates, proteins, and fats? Does every person require the same amount of calorie intake? Explain. How is the Kjeldahl method used to determine the amount of protein in a food? What two methods are used to measure fat content in food? What is the drawback of one of the methods? How is the amount of total carbohydrates in food found? How much protein, fat, and carbohydrate is it recommended that we consume? Two Is Better than One What two processes does bread undergo during toasting? When bread is toasted, what two substances react in the Maillard reactions? What type of substances are these? Explain why bread that is toasted twice can be better than bread toasted once. Describe the location and ordering of starch in potato cells. What happens to the starch when French fries are first fried? What happens to the starch when French fries are fried a second time? What is the benefit of first frying meat that will be used in a stew? Why does this not occur as meat simmers in the liquid of the stew? How does meat soften as it simmers in a stew? Whats That Smell? What are the advantages of making perfumes synthetically rather than extracting from plants and flowers? Why dont perfume manufacturers advertise the fact that 80% of perfumes are made from synthetic elements? Why might synthetic perfumes be better than those made from natural scents? In what ways are synthetic perfumes kinder to the environment and to certain animals? Why might a synthetic perfume be less likely to cause an allergic reaction? How is the gas chromatograph-mass spectrometer (GCMS) used in perfume research and development? What two properties must the mix of ingredients in a new synthetic perfume have? What is the average number of ingredients in any one commercial fragrance? Mascara: That Lush Look You Love! Name the three basic ingredients in mascara. What chemical substances are used as pigments in mascara? The article identifies six substances that are used as emollients in mascara. Name them. What are parabens? Name the group of compounds found in ancient mascara that actually protected eyes from disease. What is an emollient? Name three substances that are used as eyelash thickeners. What is guanine? Name the modern makeup company started by T. L. Williams and based on a product developed by his sister. Dirty Business: The Chemistry of Laundry Detergent Why doesnt water by itself clean clothes very well? Explain why water is a polar molecule. What are surfactants, and what effect do they have on water? What else do surfactants do, and how do they do it? What is a micelle? What role do enzymes play in cleaning clothes? What other factors besides chemical process are involved in cleaning? Whats the latest technology involved in laundry cleaning? Answers to Student Questions (from the articles) The Big Reveal: Whats Behind Nutrition Labels What information does a nutrition label on food typically contain? The label typically starts with a serving size and the number of calories per serving, followed by a list of key nutrients, including total fat, carbohydrates, and proteins. Other values may be included, such as the calories from fat, saturated fat, trans fat, dietary fiber, sugars, and various vitamins. What is the definition of a Calorie? One Calorie (kilocalorie) is the amount of energy it takes to raise 1 kilogram of water 1 C at sea level. What is the difference between a nutritional Calorie (uppercase C) and a calorie (lowercase c)? A nutritional Calorie is also called a kilocalorie, or 1,000 calories. It is sometimes written as a Calorie (uppercase C) to distinguish it from a calorie (lowercase C). How many Calories per gram are found in carbohydrates, proteins, and fats? Carbohydrates and proteins contain 4 Calories per gram and fats about 9 Calories per gram. Does every person require the same amount of calorie intake? Explain. No. How many calories you need every day varies depending on your gender, age, and activity level. How is the Kjeldahl method used to determine the amount of protein in a food? The Kjeldahl method determines the amount of ammonia that is present in a sample, which is the same as the amount of nitrogen initially present in the sample. This is then used to determine the amount of protein present in the sample. What two methods are used to measure fat content in food? What is the drawback of one of the methods? One method is the Soxhlet extraction and the other is nuclear magnetic resonance. Drawbacks of the Soxhlet extraction are that is it slow and complicated. How is the amount of total carbohydrates in food found? The amount has traditionally been calculated, rather than measured. The other components of foodsuch as proteins, fat, and waterare measured and added together. Then this sum is subtracted from the total, and the difference is assumed to be the amount of total carbohydrates. How much protein, fat, and carbohydrate is it recommended that we consume? It is recommended that 30% of our daily calories should come from fat, about 50% of calories from carbohydrates, leaving 20% of calories from protein. Two Is Better than One What two processes does bread undergo during toasting? During toasting, the bread undergoes vaporization of water and a series of chemical reactions called the Maillard reactions. When bread is toasted, what two substances react in the Maillard reactions? What type of substances are these? The Maillard reactions occur between two substances that are present in the flour used to make bread: starch and gluten. Starch is a carbohydrate, and gluten is a protein. Explain why bread that is toasted twice can be better than bread toasted once. Bread toasted twice at a lower temperature first allows some water to vaporize so the Maillard reactions can start. All the water vaporizes by the time the Maillard reactions are completed during the second toasting. Describe the location and ordering of starch in potato cells. In potato cells, starch is present in granules. In each granule, the starch molecules arrange themselves in repeating rows. What happens to the starch when French fries are first fried? Starch molecules lose their crystalline structure and become amorphous. More heating causes starch to leak out of the granules, and the granules become coated with starch. What happens to the starch when French fries are fried a second time? The starch that seeped out of the potato granules earlier starts reacting with proteins present in the potato, causing Maillard reactions that give the fries a golden brown, crispy texture. What is the benefit of first frying meat that will be used in a stew? Why does this not occur as meat simmers in the liquid of the stew? The proteins and sugars in the meat react through Maillard reactions that create compounds that add a unique combination of flavors, aroma, and appearance to the meat. Maillard reactions start at 115 oC and would not occur in the liquid which has a boiling point of roughly 100 oC. How does meat soften as it simmers in a stew? A tough meat protein called collagen reacts with water molecules that break it up into smaller molecules. The result is gelatin, a much softer compound. Whats That Smell? What are the advantages of making perfumes synthetically rather than extracting from plants and flowers? Extracting from plants and flowers is expensive, challenging, risky, and supplies can be damaged by weather or disease. Making perfumes synthetically is faster and cheaper. Why dont perfume manufacturers advertise the fact that 80% of perfumes are made from synthetic elements? Manufacturers do not like to advertise that fact because consumers believe natural is better. Why might synthetic perfumes be better than those made from natural scents? Synthetics are always the same while a natural scent depends on where a plant is grown, the weather conditions, and even the time of day. In what ways are synthetic perfumes kinder to the environment and to certain animals? Synthetics are kinder to the environment because they do not require the destruction of various plants, some of which may be in short supply. The same is true for natural perfumes that are derived from animalsthere is no need to kill or harm an animal when using a synthetic perfume that produces the same odor as a natural perfume based on some animal extract. Why might a synthetic perfume be less likely to produce an allergic reaction? A natural scent may contain hundreds of different molecules while a synthetic version contains only one or a handful which reduces the odds of a specific chemical being present that could cause an allergic reaction. How is the gas chromatograph-mass spectrometer (GCMS) used in perfume research and development? The GCMS separates out the components of a mixture (GC), and then identifies those components as well determining their amount (MS). What two properties must the mix of ingredients in a new synthetic perfume have? The perfume that is produced must be stable and have a long-lasting fragrance. What is the average number of ingredients in any one commercial fragrance? The average fragrance has some 60 to 100 ingredients, with some having more than 300. Mascara: That Lush Look You Love! Name the three basic ingredients in mascara. The article lists three basic categories of substances that make up mascarapigments, emollients and thickeners. What chemical substances are used as pigments in mascara? According to the article the typical pigments are carbon black (pure carbon), iron oxide and ultramarine blue. The article identifies six substances that are used as emollients in mascara. Name them. The article identifies typical mascara emollients as carnauba wax, beeswax, mineral oil, almond oil, castor oil, and sesame oil. What are parabens? They are a group of compounds that are esters of para-hydroxybenzoic acid, HCOOC6H4OH.  In mascara and in other cosmetics, parabens are used as preservatives. But they also have been associated with breast cancer and so have been removed from many mascara products. Name the group of compounds found in ancient mascara that actually protected eyes from disease. Certain lead chloride compounds, some of them not found in nature, were discovered in modern analyses of ancient eye makeup from Egypt. The presence of these lead compounds is known to increase nitric oxide concentrations in the body, one method of increasing the bodys immune system. What is an emollient? The article describes emollients as substances that soften and soothe eyelashes. Name three substances that are used as eyelash thickeners. Substances used as eyelash thickeners mentioned in the article are rice proteins, tapioca starch, microfibers of nylon and cellulose, and cashmere. What is guanine? Guanine is a chemical with the formula C5H5N5O. Name the modern makeup company started by T.L. Williams and based on a product developed by his sister. The name of the modern makeup company is Maybelline. Dirty Business: The Chemistry of Laundry Detergent Why doesnt water by itself clean clothes very well? Water by itself doesnt clean clothes very well because water molecules tend to attract other water molecules but not molecules of oil or grease that are present in most stains. Explain why water is a polar molecule. Water is polar because of uneven distribution of electrical charges within the molecule. The oxygen atom, which has a stronger attraction for electrons than does hydrogen, draws the electrons from the oxygen-hydrogen bonds closer to its end of the molecule making that end partially negative, while the electron-deficient hydrogen atoms end of the molecule are partially positive. What are surfactants, and what effect do they have on water? Surfactants reduce waters surface tension. This helps water spread out more on the surface of fabrics, allowing them to absorb water faster. What else do surfactants do, and how do they do it? Besides reducing waters surface tension, surfactants are also cleaning agents. The surfactant molecule is composed of two partsa polar part and a nonpolar part. The polar part is attracted to water while the nonpolar part is attracted to grease and oil, thereby lifting the stain off the fabric and allowing the wash water to rinse it away from the fabric. What is a micelle? A micelle is a cluster of surfactant molecules surrounding a nonpolar oil/grease molecule. The nonpolar ends of the surfactant molecules point in toward the oil while their polar ends point away, attracted to polar water molecules around the oil. What are enzymes and what role do they play in cleaning clothes? Enzymes are biological catalysts, which speed up chemical reactions without being changed in the process. Different enzymes attack different types of stains: proteases attack protein stains; lipases attack stains composed of lipids or fats; and amylases attack starch-based stains. All these enzymes help remove stains from clothing. What other factors besides chemical process are involved in cleaning? Besides chemical processes, mechanical processes are also involved in cleaning. Clothes must be agitated to expose the stains to surfactants and water. Heat is also almost essential to cleaning. Besides its effect of speeding up chemical reactions in the washing machine, it also increases the solubility of both detergent in the water and stains from clothing. Whats the latest technology involved in laundry cleaning? The latest technology for cleaning laundry involves nanoparticles. Nanoparticles that actually repel stains have been incorporated into fabrics. ChemMatters Puzzle: Chemical Syllabism Just as a molecule is assembled from atoms, so are words put together from syllables. In this puzzle we will show you the syllables that make up NINE common terms from the world of chemistry but scrambled! Your task is to learn the nine words. Below, we give you the syllable list (in alphabetical order) and a clue for each term. If you know it, you can cross off its syllables since each is used exactly once. The number of syllables in each is given in parentheses following the clue. We offer a few hints that may aid you. 1. The first letters, reading down, yield the last name of a famous chemist. 2. Notice that each term has one letter marked with an asterisk. When read down they identify the concept he/she is most famous for. Many of the nine terms relate to this and other varied contributions he/she made to chemistry. Your teacher can perhaps guide you to learning about them. The thirty-one SYLLABLES (in alphabetical order): AC AC BI DI DI DROX ER ES HY I IDE IZ LET NI O O ON RA RA SOL TI TION TIVE TRA TRATE UL UM UM UTES VA VI Clues: Terms: 1 . . . . . energy, described in collision theory. (4) _ _ _ _ _ _ _ _ _ _ * 2. Capable of nuclear transformation. (5) _ _ _ _ _ _ _ _ _ _ _ * 3. Element first isolated by Marie Curie.(3) _ _ _ _ _ _ * 4. (OH)1-, by name. (3) _ _ _ _ _ _ _ _ _ * 5. Its X-170 isotope has 102 neutrons. (3) _ _ _ _ _ _ * 6. Any salt of nitric acid ends in this name. (2) _ _ _ _ _ _ _ * 7. What a HCl molecule does when in water. (4) _ _ _ _ _ _ _ * 8. Beyond the blue end of the E-M spectrum. (5) _ _ _ _ _ _ _ _ _ _ _ * 9. If sea water is a solution, NaCl is one of the (2) _ _ _ _ _ _ _ Answers to the ChemMatters Puzzle The nine terms: AC-TI-VA-TION (energy) RA-DI-O-AC-TIVE HY-DROX-IDE ER-BI-UM NI-TRATE I-ON-IZ-ES UL-TRA-VI-O-LET (the) SOL-UTES Our famous chemist is Svante Arrhenius (1859 1927). He was Swedish and taught at various institutions there throughout his life. In 1903, he was awarded the Nobel Prize for Chemistry.... the third such recipient. A physical chemist, his main contribution was to show that many compounds IONIZE in water and become conducting systems. In particular, he defined acids as releasers of H+ ions and bases as releasers of OH ions. He also offered explanations for the behavior of electrolytes in causing unusually large depressions to freezing point and vapor pressure. Another area of study was in the rates of reaction, activation energy humps, and the beginnings of collision theory. NSES Correlation National Science Education Content Standard Addressed As a result of activities in grades 9-12, all students should develop understanding Nutrition LabelsTwo Is Better than OneWhats that Smell?MascaraLaundry detergentsPhysical Science Standard A: necessary to do scientific inquiry.(Physical Science Standard A: about scientific inquiry.(((((Physical Science Standard B: of the structure and properties of matter.(((((Physical Science Standard B: of chemical reactions.(((Life Science Standard C: of matter, energy, and organization in living systems.((Science and Technology Standard E: about science and technology.(((((Science in Personal and Social Perspectives Standard F: of personal and community health.(((Science in Personal and Social Perspectives Standard F: of science and technology in local, national, and global challenges.(((History and Nature of Science Standard G: of science as a human endeavor.((History and Nature of Science Standard G: of the nature of scientific knowledge((((History and Nature of Science Standard G: of historical perspectives.(( Anticipation Guides Anticipation guides help engage students by activating prior knowledge and stimulating student interest before reading. If class time permits, discuss students responses to each statement before reading each article. As they read, students should look for evidence supporting or refuting their initial responses. Directions for all Anticipation Guides: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. The Big Reveal: Whats Behind Nutrition Labels Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementA food calorie is a chemists kilocalorie, and they both measure energy.The calorie content of food was first determined in the early 1900s.Fats and carbohydrates contain the same number of Calories per gram.Dietary fiber has more calories than fats, carbohydrates, or proteins.The number of calories needed per day depends on a persons age, gender, and activity level.You can change your basal metabolic rate.Most nitrogen in foods comes from proteins.About 30% of your daily calories should come from fat.In the past, the carbohydrate content of foods has been calculated mathematically, not measured in a food science lab. About half of your daily calories should come from proteins. Two Is Better than One Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementMany foods taste better when they are cooked or heated twice.Maillard reactions to make foods brown come from fats and carbohydrates.Maillard reactions produce fewer than a thousand products.The products of Maillard reactions in foods depend on the temperature.Starch molecules are held in an orderly arrangement because of intermolecular forces.Maillard reactions occur in boiling water.Heating meat turns collagen protein into soft gelatin.Water dehydration is undesirable when making crispy toast. Whats that Smell? Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementThe first synthetic fragrances were developed in the early 1900s.The average perfume today contains less than 50% synthetic chemicals.Synthetic fragrances are better for the environment.Fragrance scientists go to exotic locations to find new scents, then they analyze them to identify the ingredients.A gas chromatograph mass spectrometer identifies molecules in a sample based on their mass.Most commercial fragrances have fewer than 50 ingredients.Smell triggers memory more than any other of our senses.Our perceptions of scents are influenced by our culture.Perfume, eau de toilette, cologne, and splash describe different concentrations of fragrances.Pheromones are processed by the vomeronasal organ, an organ humans have in common with many other mammals. Mascara: That Lush Look You Love! Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementMascara has five types of ingredients: oil, water, pigments, emollients, and thickeners.The most common pigments in mascara are carbon black and iron oxides.The guanine in mascara comes from bat and bird droppings.Parabens have been proven to cause breast cancer.Smudge-proof mascara contains fibers of nylon or rayon.In ancient times, honey was used instead of oil to make mascara stick.Mascara may have caused eye infections in ancient Egyptians.Maybelline mascara was originally made with Vaseline. Dirty Business: Laundry Comes Clean with Chemistry Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementWater alone will remove odors from clothes.Surfactants increase the surface tension of water.Surfactants have a polar and a nonpolar part.Surfactant molecules surround stains so they can be lifted off the fabric being washed.Enzymes in laundry detergents usually remove only fats.A little saliva may help remove a stain.Agitation is unnecessary to remove dirt from clothes.Heat speeds up the chemical reactions involving enzymes, helping to remove dirt and stains.In the future, nanoparticles may make detergent obsolete. Reading Strategies These matrices and organizers are provided to help students locate and analyze information from the articles. Student understanding will be enhanced when they explore and evaluate the information themselves, with input from the teacher if students are struggling. Encourage students to use their own words and avoid copying entire sentences from the articles. The use of bullets helps them do this. If you use these reading strategies to evaluate student performance, you may want to develop a grading rubric such as the one below. ScoreDescriptionEvidence4ExcellentComplete; details provided; demonstrates deep understanding.3GoodComplete; few details provided; demonstrates some understanding.2FairIncomplete; few details provided; some misconceptions evident.1PoorVery incomplete; no details provided; many misconceptions evident.0Not acceptableSo incomplete that no judgment can be made about student understanding Teaching Strategies: Links to Common Core State Standards: Ask students to develop an argument about using synthetic fragrances, mascara, or laundry detergents. In their discussion, they should state their position, providing evidence from the articles to support their position. If there is time, you could extend the assignment and encourage students to use other reliable sources to support their position. Vocabulary that may be new to students: Calories Metabolism Maillard reaction Pheromones Surfactant Micelle Enzyme The Big Reveal: Whats Behind Nutrition Labels Directions: As you read the article, complete the chart below comparing proteins, carbohydrates, and fats in our food. NutrientCalories/ gramFoods containing this nutrientHow amount of this nutrient is determinedPercent of nutrient needed dailyProteinsCarbohydratesFats Two Is Better than One Directions: As you read, complete the chart below comparing the cooking temperatures, Maillard reactions, and other processes involved in cooking toast, French fries, and meat. FoodTemperatures neededChemicals involved in Maillard reactions Other chemical or physical processes involvedToast1. 2.French fries 1. 2.Meat1. 2. Whats that Smell? Directions: As you read, describe the importance of each concept on the left, along with how fragrance scientists use this concept to their advantage. Use or importanceAdvantagesSyntheticsGCMSMixing chemicalsPersonal experiencePheromones Mascara: That Lush Look You Love! Directions: As you read, complete the chart below to describe the substances found in mascara. Substances ExamplesWhy is (or was) it used?PigmentsEmollientsThickener ParabensAncient mascara Dirty Business: Laundry Comes Clean with Chemistry Directions: As you read, complete the chart below, describing the chemistry involved in cleaning your clothes. Substance or ProcessWhy does this help get clothes clean? Is it always necessary?Water SurfactantEnzymesAgitationHot waterFuture: Nanoparticles The Big Reveal: Whats Behind Nutrition Labels Background Information (teacher information) More on nutritional labeling Since the introduction of nutritional labeling on food products, the form of the labels and the information required to appear on them have evolved over the years and continue to change as new information arises regarding foods, nutrients, and their links with diet and overall health. The extensive 2010 report Examination of Front-of-Package Nutrition Rating Systems and Symbols: Phase I Report ( HYPERLINK "http://www.nap.edu/catalog.php?record_id=12957" http://www.nap.edu/catalog.php?record_id=12957) outlines the history of labeling in great detail. Excerpts that highlight the major events are: Up to the late 1960s, there was little information on food labels to identify the nutrient content of the food. From 1941 to 1966, when information on the calorie or sodium content was included on some food labels, those foods were considered by the Food and Drug Administration (FDA) to be for special dietary uses, that is, intended to meet particular dietary needs caused by physical, pathological, or other conditions. At that time meals were generally prepared at home from basic ingredients and there was little demand for nutritional information. However, as increasing numbers of processed foods came into the marketplace, consumers requested information that would help them understand the products they purchased. In response to this dilemma, a recommendation of the 1969 White House Conference on Food, Nutrition, and Health was that FDA consider developing a system for identifying the nutritional qualities of food Then in 1972 the agency proposed regulations that specified a format to provide nutritional information on packaged food labels. Inclusion of such information was to be voluntary, except when nutrition claims were made on the label, in labeling, or in advertising, or when nutrients were added to the food. When finalized in 1973, these regulations specified that when nutrition labeling was present on the labels of FDA-regulated foods, it was to include the number of calories; the grams of protein, carbohydrate, and fat; and the percent of the U.S. Recommended Daily Allowance (U.S. RDA) of protein, vitamins A and C, thiamin, riboflavin, niacin, calcium, and iron. Sodium, saturated fatty acids, and polyunsaturated fatty acids could also be included at the manufacturers discretion. All were to be reported on the basis of an average or usual serving size. few changes were made in nutrition labeling regulations over the next decade. In August 1987, FDA published a proposed rule to change its policy by permitting health claims on food labeling if certain criteria were met. A congressional hearing was also held in December 1987. Subsequently, in February 1990, FDA withdrew its original proposal and published a new proposal that defined appropriate health claims more narrowly and set new criteria to be met before allowing a claim. The surge in consumer interest in nutrition that was fueling the food industrys desire to highlight the positive nutritional attributes of food products was due, in part, to the publication in the late 1980s of two landmark consensus reports on nutrition and health. The Surgeon Generals Report on Nutrition and Health (HHS, 1988) and the National Research Councils (NRCs) report Diet and Health: Implications for Reducing Chronic Disease Risk (NRC, 1989) emphasized the relationship between diet and the leading causes of death among Americans (e.g., heart disease, cancers, strokes, and diabetes). These reports made useful suggestions for planning healthy diets. However, without specific nutrition information on food labels, consumers were unable to determine how certain individual foods fit into dietary regimens that followed the recommendations of these reports. Major changes in nutrition labeling were necessary if food labels were to be useful to consumers interested in adhering to these recommendations. Congressional concerns about food labeling had been building for some time. This culminated in November 1990 with the passage of the NLEA [Nutritional Labeling and Education Act], the most significant food labeling legislation in 50 years. The NLEA amended the Federal Food, Drug, and Cosmetic Act to give FDA explicit authority to require nutrition labeling on most food packages and specified nutrients to be listed in the nutrition label. It also required that nutrients be presented in the context of the daily diet; specified that serving sizes should represent an amount customarily consumed and which is expressed in a common household measure that is appropriate to the food; and provided for a voluntary nutrition labeling program for raw fruits, vegetables, and fish. It also required standard definitions to be developed that characterized the level of nutrients and required that FDA provide for approved health claims. The NLEA pertains only to those labels of food products regulated by FDA, which has label authority over the majority of foods. (pp 1921, 23) Various additional rules have been passed since then, summarized in Table 2-1 of the previously quoted report. ( HYPERLINK "http://www.nap.edu/catalog.php?record_id=12957" http://www.nap.edu/catalog.php?record_id=12957) For example, a rule was finalized in 2003 requiring the addition of trans fatty acids to nutrition labeling (p 36). The specifics of what a label must include and how it must appear are quite detailed and are summarized at  HYPERLINK "http://www.fda.gov/ICECI/Inspections/InspectionGuides/ucm074948.htm" http://www.fda.gov/ICECI/Inspections/InspectionGuides/ucm074948.htm. For example, Nutrition information must be set off in a box. The format requires that headings and nutrients be separated by bars. Nutrition Facts must be presented in bold print and in print larger than any other printed information in the nutrition label. Calories must be in bold print. Calories from Fat must be declared unless the product contains < 0.5 g total fat. Calories may be followed by the optional term Energy in parenthesis. There is even an order for listing optional vitamins and minerals. If a manufacturer wishes to make a nutrient content claim such as calorie free, low calorie, reduced calories, less calories, light, or lite, there are definitions that must be met for the claim to be made. Definitions can be seen at  HYPERLINK "http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/FoodLabelingNutrition/FoodLabelingGuide/ucm064911.htm" http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/FoodLabelingNutrition/FoodLabelingGuide/ucm064911.htm. While the FDA sets guidelines for nutritional labeling, the manufacturers themselves are responsible for providing accurate nutritional information. A blog post on the Decoded Science Web site by Janelle Vaesa, who has a Master of Science degree in Public Health, includes information from an interview with an FDA spokesperson. One interview quote from the spokesperson is: FDA does not tell companies how to generate their nutrition information. FDA does not approve labels on food products. It is the responsibility of the firm to assure that the nutrition information is accurate. However, FDA does have a compliance program with a sampling plan. Vaesa states, Food manufacturers can determine the nutrients in a product by comparing ingredients to a database where averages for foods are listed, or by sending samples for lab testing. The remaining portion of the blog describes a diabetic consumer who ate a low-carbohydrate bread product, had abnormally high blood sugar levels afterward, and decided to send the bread for testing to see if its nutritional information was accurate; it was not. ( HYPERLINK "http://decodedscience.com/nutritional-labeling-fda-oversight-not-what-youd-expect/15446" http://decodedscience.com/nutritional-labeling-fda-oversight-not-what-youd-expect/15446) The Tinnesand article discusses the work Wilbur O. Atwater did to determine the calorie content of food using a respiration calorimeter. Atwater has been called the father of American nutrition science ( HYPERLINK "http://www.ars.usda.gov/is/timeline/nutrition.htm" http://www.ars.usda.gov/is/timeline/nutrition.htm); while much more knowledge and sophisticated methods of analysis have since been discovered, his work on the chemical analysis of food was an important beginning. Various sources describe Atwaters work. He had a particular talent for encouraging support of his work, both through acquiring funding from multiple sources and by effectively demonstrating his work to the public. A 2009 article in The Journal of Nutrition describes his legacy: ( HYPERLINK "http://jn.nutrition.org/content/139/1/178.full.pdf+html" http://jn.nutrition.org/content/139/1/178.full.pdf+html) The systematic chemical analysis of food for human consumption in the United States had its origin with Wilbur O. Atwater. As early as the late 1860s, Atwater, then a student at Yale University, conducted a series of analyses of the composition of Indian corn. Atwater continued this research while at Wesleyan University and also as a scientist of the Storrs (Connecticut) Experiment Station in support of food consumption surveys and metabolic research that he was conducting. Although Atwater received direct funding as part of his role as Chief of Human Nutrition Investigations, USDA Office of Experiment Stations while at Wesleyan, he also had a unique ability to acquire support for his research from a variety of other sources. With regard to food composition activities, the Smithsonian Institution, United States Fish Commission, and United States National Museum were a few of the organizations that funded his research. Atwater also took advantage of timely events for his research. As an example, he collected a large number of national and international foods for analyses while at the Chicago Worlds Fair (1893) and, at the same time, secured over 500 samples of meats and meat products from the many local slaughterhouses. Atwater made sure the resulting data were widely distributed in a variety of publications, some of which are available on the USDA Nutrient Data Laboratory Web site. ( HYPERLINK "http://www.ars.usda.gov/nutrientdata" http://www.ars.usda.gov/nutrientdata) (p 178) Part of Atwaters work included studies of the amounts and type of nutrients people need to function at their best; this entailed studies of human metabolism and respiration. ( HYPERLINK "http://www.ars.usda.gov/is/timeline/nutrition.htm" http://www.ars.usda.gov/is/timeline/nutrition.htm) The 1993 W. O. Atwater Centennial Memorial Lecture ( HYPERLINK "http://jn.nutrition.org/content/124/9_Suppl/1707S.long" http://jn.nutrition.org/content/124/9_Suppl/1707S.long) describes one such study: a request from the Massachusetts Bureau of Labor to evaluate and comment on the data that they had been collecting as to the food consumed by working people, both in family homes and boarding houses. At that time food purchases could take up 60% of a familys budget. Atwaters work helped to determine what foods could give this audience of eaters the most bang for the buck. He published this work in a series of articles in a magazine read by the middle class public. Their bottom line was that the poor could eat more cheaply by using more economical sources of protein and margarine in place of butter, and thus be better off. A short piece published in The New York Times on November 16, 1888, discusses a lecture titled The Chemistry and Economy of Food delivered by Atwater the previous evening, covering this same subject. The article states, Men of limited means often buy high-priced meats at the same market where a wealthier neighbor buys cheaper cuts and gets as much or even more nutriment. ( HYPERLINK "http://query.nytimes.com/gst/abstract.html?res=F00E10FF385413738DDDAF0994D9415B8884F0D3" http://query.nytimes.com/gst/abstract.html?res=F00E10FF385413738DDDAF0994D9415B8884F0D3) His study of nutrition in foods even extended to alcohol: The finding from the calorimeter work that received most attention in Atwaters lifetime concerned alcohol. In 1899 he reported that if a subject drank alcohol equivalent to that in a 750 ml bottle of wine, and in small portions over the course of the day, it was almost fully oxidized and replaced the caloric equivalent of either fat or carbohydrate. In other words, at this level, it acted as a food. This was immediately taken up by the liquor trade and used in their advertising. ( HYPERLINK "http://jn.nutrition.org/content/124/9_Suppl/1707S.long" http://jn.nutrition.org/content/124/9_Suppl/1707S.long) More on calories  The Tinnesand article includes the definition of the nutritional calorie, or Calorie, or kilocalorie: the amount of energy it takes to raise 1 kilogram of water 1 C at sea level. Students used to only seeing the calorie label on products might not make the connection between this word and the idea that it is a measure of an amount of energy. Nutritional labels in Europe are slightly different than those found in the U.S. and the units and phrases used in Europe help to highlight this idea. The article Nutrition Labeling to Prevent Obesity: Reviewing the Evidence from Europe describes nutrition labeling in Europe. It states that the new nutrition labeling regulation decided on in December 2011 maintains the requirement to display energy in both kilojoules (kJ) and kilocalories (kcal) (there are 4.2 kJ in each kcal). (DOI 10.1007/s13679-012-0020-0,  HYPERLINK "http://link.springer.com/article/10.1007/s13679-012-0020-0" http://link.springer.com/article/10.1007/s13679-012-0020-0) Students might be interested to compare food labels from different countries, if available, and determine any differences. A label from a package of basmati rice sold in the United Kingdom is shown in this Teachers Guide. Seeing the phrase Energy Value along with the kilojoule unit students might typically see in a physical science classroom while performing energy calculations helps to highlight this idea. The calorie unit can be somewhat confusing. Even though a nutritional calorie is a kilocalorie or a Calorie, it is not normally written as such for convenience, instead using just calorie. The October 2000 ChemMatters Question from the Classroom column included a brief calculation along with comments about this common confusion: Lets say you went outside on a cold day and your body temperature dropped from its normal 37.0 C to a slightly chilly 35.0 C. Assuming you weigh about 70 kg and assuming your specific heat capacity to be fairly close to that of wateryour main ingredientthen it would take approximately 70,000 g x 1 cal/g C x 2.0 C = 140,000 cal of food consumption just to bring your body temperature back to normal. But a small apple only provides about 70 Calories of energy. Are we talking about chowing down 2000 apples just to warm yourself up a few degrees? Not at all. Remember, a food Calorie is spelled with a capital C, representing the prefix kilo. Actually, an apple has about 70,000 calories in it. Fortunately, it would require only two apples worth of energy to warm you back up. (p 2) (Becker, R. Question from the Classroom. ChemMatters, 2000, 18 (3), p 2) A reminder of where the energy in food comes from may make students think theyre back in the biology classroom as they hear the word photosynthesis, but chemistry is in the mix as the energy needed to break bonds and the energy released as bonds are formed are brought into the discussion. The section Energy Flow: From the Sun to You in the Chemistry in the Community textbook (5th ed., American Chemical Society: Washington DC, 2006) discusses photosynthesis: All food energy originates from sunlight. Through photosynthesis, green plants capture and use solar energy to make large molecules from smaller, simpler ones. green plants, through photosynthesis, use solar energy to convert water and carbon dioxide into carbohydrates and oxygen gas. Although a variety of carbohydrates are produced, an equation for photosynthesis usually depicts the production of glucose: 6 CO2 + 6 H2O + Solar energy ( C6H12O6 + 6 O2 For this reaction to occur, bonds between the carbon and oxygen atoms in carbon dioxide molecules and between the oxygen and hydrogen atoms in water molecules must be broken. The atoms must then recombine in a different arrangement to form glucose and oxygen molecules. breaking bonds always requires energy, whereas bond formation releases energy. In photosynthesis, the bonds in carbon dioxide and water molecules require more energy to break than is released when chemical bonds in glucose and oxygen form. The energy needed to drive this endothermic reaction comes from the Sun. (pp 572573) Humans are able to make use of these carbohydrates both through direct consumption of plants, along with consumption of other animals that also eat plants. A 2005 report by the Institute of Medicine of the National Academies ( HYPERLINK "http://www.nal.usda.gov/fnic/DRI/DRI_Energy/energy_full_report.pdf" http://www.nal.usda.gov/fnic/DRI/DRI_Energy/energy_full_report.pdf) describes the bodys energy usage from this food intake: Humans and other mammals constantly need to expend energy to perform physical work; to maintain body temperature and concentration gradients; and to transport, synthesize, degrade, and replace small and large molecules that make up body tissue. This energy is generated by the oxidation of various organic substances, primarily carbohydrates, fats, and amino acids. Chemistry in the Community (5th ed., American Chemical Society: Washington DC, 2006) also states: You may be surprised to learn that only a small fraction (about 1015%) of food energy consumed by organisms is used for growthfor converting smaller molecules to larger molecules that become part of an animals structure. Over half the energy contained in consumed food is used to digest food molecules. The supply of useful energy declines as energy continues to transfer away from its original sourcethe Sun. (p 573) The Institute of Medicine report Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids mentioned above establishes a set of reference values for dietary energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids to expand and replace previously published Recommended Dietary Allowances (RDAs) for the United States (p xv), using data from observational and experimental studies (p 2). ( HYPERLINK "http://www.nal.usda.gov/fnic/DRI/DRI_Energy/energy_full_report.pdf" http://www.nal.usda.gov/fnic/DRI/DRI_Energy/energy_full_report.pdf) The report, at over 1300 pages, is a fairly exhaustive treatment of our nutritional requirements. Its data helps to form the basis of the numbers seen in the table of calorie needs by gender, age, and activity level in the Tinnesand article. More on protein The December 2011 ChemMatters article Hold the Meat!: Meat-Free Food Takes a Seat at the Table discusses the function of the proteins that we eat. The rest of the article goes on to describe several meat-free food products made of plant-based proteins as options for a vegetarian diet. Proteins are abundant not only in meat but also in milk, eggs, and legumes. When we eat any of these food groups, our body breaks them down during digestion, and proteins are broken down into their basic components, which are called amino acids. Amino acids are nutritionally important for building new proteins that you need for growth, immune function, tissue repair, and manufacturing of enzymes and hormones. Also, the nitrogen provided by amino acids plays a role in your genetics, as a part of molecules called nucleic acids, such as deoxyribonucleic acid (DNA). Living beings on Earthplants, animals, bacteria, and so forthcan produce 20 different types of amino acids, but humans can synthesize only 11 of these 20 amino acids. The other nine, called essential amino acids, cannot be made in our bodies and must come from the food we eat. The proteins in our bodies are made of different types of amino acids, but any given protein may not contain all 20 amino acids. Nutritionists used to recommend combining foods, especially in vegetarian diets, so that all essential amino acids were present at once. Scientists now know that people do not have to eat all essential amino acids together in one meal. Whats important is to eat a balanced amount of proteins with different types of essential amino acids during the day. Plant-based proteins tend to contain a lower amount of essential amino acids than animal proteins. But by eating a variety of legumes, grains, nuts, fruits, and vegetables, the bodys amino acid needs are easily met. In fact, according to the World Health Organization, the average American consumes double the amount of protein needed for healthy bodily function. You can estimate the amount of protein you need per day in grams by multiplying your body weight in pounds by 0.36 for teenagers or 0.4 for an active adult. (p 9) The daily amount of protein required for a 160-pound adult mentioned in the Tinnesand article is 58 grams. This amount can be roughly met by eating cereal with milk for breakfast, a peanut butter and jelly sandwich for lunch, and a piece of fish with a side of beans for dinner. ( HYPERLINK "http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/protein-full-story/index.html" http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/protein-full-story/index.html) The same Web site describes the effects of protein malnutrition, which affects millions of people around the world: growth failure, loss of muscle mass, decreased immunity, weakening of the heart and respiratory system, and death. In the United States, an issue that is more likely to come up is eating too much protein rather than too little. A sidebar at  HYPERLINK "http://www.cdc.gov/nutrition/everyone/basics/protein.html" http://www.cdc.gov/nutrition/everyone/basics/protein.html answers the question Is there any harm in getting more protein than I need? It answers: Most people eat more protein than they need without harmful effects. However, protein contributes to calorie intake, so if you eat more protein than you need, your overall calorie intake could be greater than your calorie needs and contribute to weight gain. Besides that, animal sources of protein can be sources of saturated fat which has been linked to elevated low-density lipoprotein (LDL) cholesterol, a risk factor for heart disease. In addition, for people with certain kidney diseases, a lower-protein diet may be recommended to help prevent an impairment in kidney function. The Kjeldahl method, described in the Tinnesand article, is used to determine the protein content of foods. The method was developed by a Danish chemist, Johan Kjeldahl, in connection with his work at the Carlsberg Laboratory in Copenhagen, Denmark. The laboratory was an offshoot of a laboratory previously researching brewing processes for the Carlsberg Brewery. The new institute was not limited to science related only to the brewing industry, although Kjeldahls test did arise from his work on proteins and their transformations during the germination of barley and the alcoholic fermentation of beer wort (p 460, Veibel, S.; J. Chem. Educ. 1949, 26 (9), pp 459461). It was designed to assist his studies into the protein content of various grains used for brewingless protein meant more beer. He needed to measure nitrogen as this is one of the major elements found in protein, but existing techniques were unreliable or inaccurate. ( HYPERLINK "http://www.carlsberggroup.com/Company/Research/Pages/Kjeldahl.aspx" http://www.carlsberggroup.com/Company/Research/Pages/Kjeldahl.aspx) He first published the method in 1883 as a means for determining the nitrogen content of organic substances and it was widely accepted as a superior method. The J. Chem. Educ. article author even says, Kjeldahl has been verbalized, an honor not usually accorded to a chemist. You do not say that the nitrogen content of a substance was determined by the Kjeldahl method, but mention simply that the substance was kjeldahled, and no chemist will misunderstand you (p 460). The three steps of the Kjeldahl method are outlined in the Tinnesand article. They can be abbreviated to say that the process involves 1) digestion of the sample, 2) distillation, and 3) titration. A brief description of each is found at  HYPERLINK "http://www.foodquality.com/details/article/814365/Protein_Determination_in_Cereals_and_Seeds.html?tzcheck=1" http://www.foodquality.com/details/article/814365/Protein_Determination_in_Cereals_and_Seeds.html?tzcheck=1: Digestion of the sample: The most time-consuming step in the analysis, this is designed to break down the bonds that hold the polypeptides together and convert them to simpler chemicals such as water, carbon dioxide, and ammonia. Adding strong sulfuric acid and heating the mixture to about 370C to 400C for 60 to 90 minutes oxidizes the organic material and releases ammonium ions. Distillation: This separates the ammonia from the digestion mixture by raising the pH with sodium hydroxide, which changes the ammonium ions into ammonia gas. The ammonia is collected through boiling and distillation of the gas into a trapping solution of hydrochloric acid. Titration: As the ammonia dissolves into the trapping solution, it is back-titrated so that the quantity of distilled-off ammonia can be calculated and the amount of nitrogen in the protein determined. The same site also discusses one of the drawbacks of the process, that the digestion step takes a significant amount of time. It says the use of catalysts such as mercury and selenium have been used in an effort to speed up the process, but the reaction remains time-consuming, inefficient, and costly on a large scale. The method is labeled as a test for protein; however, it does not test protein content directly. Rather, it measures the amount of nitrogen present in a sample. The adjustment is described in a Journal of Chemical Education article: By assuming virtually all nitrogen comes from the peptide bonds of protein molecules, a correlation to protein content can be made, using a conversion factor. A typical conversion factor is 6.25 (equivalent to 0.16 g of nitrogen per gram of protein); however, this conversion factor is only approximate as different amino acid compositions can present different nitrogen content. Different conversion factors are used in different food analyses as information about the specific amino acid composition is considered. Without knowing the exact amino acid composition of a given foodstuff (which is often extremely difficult, expensive, and unnecessary), the protein determination remains an approximation. The percent protein measured in these procedures, known as crude protein, also fails to account for other nitrogen-containing nonprotein molecules in the original sample (p 497, Kimbrough, D. R.; Jensen, A. C.; J. Chem. Educ. 2010, 87 (5), pp 496499). The focus of the Journal of Chemical Education article is integrating a discussion of melamine contamination of foods into the classroom. Melamine has been added by corrupt manufacturers to increase the apparent values of protein in foods. However, it is only safe in low doses. Reports of sickness and deaths from contamination of pet food and infant formula were in the news in 2007 and 2008. More on fats Calling a food fatty can often be a derogatory term, but fat in appropriate amounts is a needed part of our diet. A past ChemMatters article (Ruth, C. A Calorie-Free Fat?, ChemMatters, 1999, 17 (2), pp 911) mentions a connection of our love of fatty foods with its energy benefits: Some scientists believe that the reason humans get such pleasure from fat-filled food is that primitive huntergatherer societies had unpredictable food sources; those with a taste for calorie-rich fat were more likely to survive, because fats have more calories per gram (9 cal/g) than carbohydrates or even proteins. The textbook Chemistry in the Community (5th ed., American Chemical Society: Washington DC, 2006) states several reasons our bodies need fat: Fats provide essential fatty acids, which function in growth and development, so they are especially important to infants and toddlers. Fat also helps in absorption of fat-soluble vitamins. Fat serves as an efficient way to store excess food energy. (p 589) The 2000 ChemMatters article FatsFitting Them Into a Healthy Diet provides a summary of fats and their different structures (Banks, P. FatsFitting Them Into a Healthy Diet, ChemMatters, 2000, 18 (3) pp 68): The term fat does not refer to one particular molecule, but rather to a large number of possible molecules with similar structures. All fat moleculeswhether in solid or liquid fatare formed by attaching three molecules of fatty acids to one molecule of glycerol. Fats get their special chemical properties and health effects from the kinds of fatty acids they contain. Fatty acids are carbon chains that may have from 3 to 18 carbon atoms. The chain may also contain one or more carboncarbon double bonds. Fatty acids are called polyunsaturated if there are two or more double bonds; monounsaturated, if there is one, and saturated, if there are none. Saturated means that the carbon atoms in the hydrocarbon chain are bonded to the maximum possible number of hydrogen atomsnot the case when there are double bonds present. Polyunsaturated fats like corn and safflower oil and monounsaturated fats like olive and canola oil tend to be liquids at room temperature. Saturated fats like butter and lard are solids. A diet high in saturated fat tends to raise levels of cholesterol in your blood, causing deposits to form in the walls of blood vessels like rust in old pipes. One important step toward lowering the risk of heart disease is to reduce the amount of trans fatty acids we consume. When a carboncarbon double bond exists in a hydrocarbon chain, there are two different ways of arranging the hydrogen atoms attached to the two carbons. They can be placed on the same side of the double bond, an arrangement called the cis configuration, or they can be placed on opposite sides of the carboncarbon double bondthe trans configuration. Natural unsaturated fats have double bonds in the cis configuration. During food manufacturing, however, that cis configuration can be altered. In preparing many productsmargarine, for examplemanufacturers expose polyunsaturated oils to hydrogen. This process, called hydrogenation, is used to convert the liquid oil to a solid spreadable product. Hydrogenation eliminates some of the double bonds by saturating them with hydrogen. The remaining double bonds are converted to the trans configuration. Several studies now suggest that trans fatty acids tend to raise blood cholesterol levels more than cis fatty acids, although not as much as saturated fats. Based on these studies, authorities like the American Heart Association have recommended that people try to use oils that havent been hydrogenated. (pp 67) The review article Comparisons of NMR/MRI Technique with Other Analytical Methodologies in the Field of Food Science and Technology ( HYPERLINK "http://www.worldfoodscience.org/cms/?pid=1006201" http://www.worldfoodscience.org/cms/?pid=1006201) highlights several studies that compare nuclear magnetic resonance (NMR) data with other tests typically used to measure values of food samples such as moisture content, fat content, protein content, etc. For fats, it compares NMR with a Soxhlet extraction, which has long been a standard test for determining fat content, stating that results were very comparable. The article also outlines the advantages of NMR, such as: NMR/MRI requires minimal or no sample preparation NMR/MRI permits the quick easy-handling and measurement of large amounts of small sample sizes in a short time NMR/MRI analysis does not produce hazardous wastes therefore being environmentally friendly and disadvantages: the high cost of NMR/MRI instruments and its maintenances NMR/MRI require sophisticated data analysis there is a lack of NMR/MRI equipment specifically designed for food purposes It recommends pairing NMR analysis with other methods. More on carbohydrates As the Tinnesand article states, about half of our daily calorie intake should come from carbohydrates. A distinction can be made between good and bad carbohydrates when making selections to meet this intake. The Harvard School of Public Health advises: Choose the best sources of carbohydrateswhole grains (the less processed, the better), vegetables, fruits and beanssince they promote good health by delivering vitamins, minerals, fiber, and a host of important phytonutrients. Skip the easily digested refined carbohydrates from refined grainswhite bread, white rice, and the likeas well as pastries, sugared sodas, and other highly processed foods, since these may contribute to weight gain, interfere with weight loss, and promote diabetes and heart disease. ( HYPERLINK "http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/carbohydrates/" http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/carbohydrates/) Another way of judging which carbohydrates to choose is by looking at a foods glycemic index (GI). The April 2004 ChemMatters teachers guide discussed GI: There are good and bad carbohydrates, depending on how quickly that carbohydrate is converted to glucose in the bloodstream, as measured by the glycemic index (GI). The GI rates glucose at 100, and foods are ranked according to how fast they enter the bloodstream, relative to glucose. For example, if a food has a GI of 50, it is absorbed into the bloodstream half as fast as that of glucose. Good carbohydrates have a low GI, and bad carbohydrates have a high GI. White bread, for example, has a GI of 70, while pumpernickel has a GI of 41. Generally, the more refined the food, the quicker it can be broken down and the higher its GI. The more work our body must do to break down a substance, the slower it will be absorbed into the bloodstream. The GI is not a perfect measure of what foods to eat, however. Fructose, for example, has a GI of only 20. Scientists actually give rats large doses of fructose to make them insulin resistant. (p 20) Foods with adequate fiber are another recommendation. Plants such as fruits and vegetables are quality carbohydrates that are loaded with fiber. Studies show an increased risk for heart disease with low-fiber diets. There is also some evidence to suggest that fiber in the diet may help to prevent colon cancer and promote weight control. ( HYPERLINK "http://www.webmd.com/food-recipes/features/carbohydrates" http://www.webmd.com/food-recipes/features/carbohydrates) Fiber also helps us feel fuller, for longer. Carbohydrates are described in the October 2011 ChemMatters Teachers Guide: In a biochemical context the term carbohydrate is often used interchangeably with the term saccharide. There are four classes of saccharidesmonosaccharides, disaccharides, oligosaccharides and polysaccharides. Monosaccharides are simple (lower molecular weight) sugars like glucose. Disaccharides are two monosaccharide molecules joined chemically by a covalent bond. Sucrose, or table sugar, and lactose are examples of disaccharides. Sucrose is composed of the monosaccharides glucose and fructose as described in the [October 2011] article. Lactose is made up of sucrose and galactose. It should be noted that if monosaccharide units like glucose are bonded together to make longer chains, the resulting heavier molecules are properly called polymers. So polysaccharides are really natural polymers. If the number of monosaccharide units is less than about ten, the polymer is called an oligosaccharide, and longer polymer saccharide chains are called polysaccharides. These heavier polysaccharides have varying biological uses, including starch in plants and glycogen in animals. Both are used to store energy. For example, the article refers to glycogen as the compound that is produced from glucose in order to store excess amounts of the glucose molecule. Even longer and heavier polysaccharides like cellulose are the primary components of cell walls in plants. About a third of all plant matter is cellulose. So simple sugars like glucose can be thought of as monosaccharides, and complex sugars like sucrose can be thought of as disaccharides. Starch and fiber, mentioned above, are polysaccharides. (p 27) Connections to Chemistry Concepts (for correlation to course curriculum) ThermodynamicsThe conversion of solar energy to energy stored in plants through photosynthesis, and the use of that stored energy by herbivores and others higher up the food chain can be discussed in connection with thermodynamics, exothermic/endothermic reactions, and Hesss Law. ThermochemistryCalories on food labels are a natural segue into a discussion of exothermic/endothermic reactions and heats of reaction. BiochemistryThree types of biochemically important molecules are discussed in the articleproteins, fats, and carbohydrates. The structures and functions of these in the human body could be discussed. Analytical ChemistryThe use of analytical testing processes to quantitatively determine the amount of proteins and fats is discussed in the article. Acid-base chemistryThe Kjeldahl method of determining the amount of protein in food involves the use of acid base reactions; in fact, all three reactions could be construed to be acid-base reactions. The first uses the sample and H2SO4, and produces (NH4)2SO4 and CO2 and SO2. The NH4+ ion is weakly acidic and the other two are both acid anhydrides. The second equation reacts the NH4+ ions with NaOH, a strong base, and produces another weak acid and weak base (H2O and NH3). The third equation reacts boric acid, B(OH)3, with the weak base ammonia, NH3. Possible Student Misconceptions (to aid teacher in addressing misconceptions) A calorie is the same as a Calorie. The capitalization can make a difference! Sometimes writing the word as a Calorie is used to distinguish it as a nutritional calorie, or a kilocalorie, or the amount of energy to raise one kilogram of water one degree Celsius at sea level. This can be confusing for students who have used the calorie unit, meaning the amount of energy to raise one gram of water one degree centigrade at sea level, in physical science problems. All components of foodfat, protein, and carbohydrateshave the same calorie content, so it doesnt matter which I eat. Fats provide 9 Calories per gram, while proteins and carbohydrates provide 4 Calories per gram. Anticipating Student Questions (answers to questions students might ask in class) What are empty calories? Empty calories are calories from solid fats and/or added sugars. These add calories but few or no nutrients. Some examples of empty calorie foods are soda and most candies. ( HYPERLINK "http://www.choosemyplate.gov/weight-management-calories/calories/empty-calories.html" http://www.choosemyplate.gov/weight-management-calories/calories/empty-calories.html) Are all fats bad? No. Some fat is necessary in our diets. It supplies fatty acids for building structures such as cell membranes. Certain fatty acids cannot be produced by our bodies and must be taken in as part of our diet. It allows the absorption of fat-soluble vitamins A, D, E, and K. It is also a major source of energy. However, a distinction is made between good (polyunsaturated fats and monounsaturated fats) and bad (saturated fats and trans fats) fats. In-class Activities (lesson ideas, including labs & demonstrations) Students could compare the nutritional labels of a food product that is sold as both a regular version and a fat-free or reduced-fat version. Some may think that if a product is fat-free it gives one license to eat more of it. However, fat-free does not necessarily mean that a serving is much lower in calories. A chart at  HYPERLINK "http://www.nhlbi.nih.gov/health/public/heart/obesity/lose_wt/fat_free.htm" http://www.nhlbi.nih.gov/health/public/heart/obesity/lose_wt/fat_free.htm compares the calories contained in identical serving sizes of the types of several products. Students could calculate the calories and grams of fat, protein, and carbohydrates in a meal they would typically order at a fast food restaurant and compare it to recommended amounts for their age and activity level. For example, McDonalds has nutrition facts for many of its menu items at  HYPERLINK "http://nutrition.mcdonalds.com/getnutrition/nutritionfacts.pdf" http://nutrition.mcdonalds.com/getnutrition/nutritionfacts.pdf. The ChemSource 3.0 module Food and Chemistry has two experiments relevant to this article: An experiment to determine the mass of sodium chloride in a package of corn chips using the Mohr chloride titration method. Students can compare the experimental value with the one stated on the nutritional label. (pp 39) An experiment to determine the relative amounts of water, fats, proteins, and carbohydrates in a milk sample. (pp 1522) The Tinnesand article mentions a method for determining the amount of protein in food. Students can perform a much simpler test to qualitatively determine the presence of protein in food. The biuret test detects the presence of peptide bonds present in proteins. One such lab procedure is available online at  HYPERLINK "http://www.foodafactoflife.org.uk/attachments/d631ae01-38c9-47918f0f5869.pdf" http://www.foodafactoflife.org.uk/attachments/d631ae01-38c9-47918f0f5869.pdf. A similar experiment, Tests for Protein, is also available in the book Chemical Activities, pp 217218 (Borgford, C. L.; Summerlin, L. R. Chemical Activities, Teacher Ed., American Chemical Society, Washington DC, 1988). Students can make glue using milk by separating out the protein casein from the milk. One procedure is available online at  HYPERLINK "http://www.rsc.org/learn-chemistry/content/filerepository/CMP/00/000/529/cce-75.pdf?v=1349533065308" http://www.rsc.org/learn-chemistry/content/filerepository/CMP/00/000/529/cce-75.pdf?v=1349533065308. Students can determine and compare the melting point of different fats, such as lard, margarine, and butter, using capillary tubes and standard lab equipment. The handout at  HYPERLINK "http://www.foodafactoflife.org.uk/attachments/75a1315c-5607-4c6dd971c68b.pdf" http://www.foodafactoflife.org.uk/attachments/75a1315c-5607-4c6dd971c68b.pdf describes the procedure. Students can determine the amount of fat in a sample of ground beef and compare it to the percentage listed on the package. A sample is weighed, and then boiled in a beaker of water for 10 minutes. The beaker is removed from the heat and allowed to cool. The top fat layer is poured into a graduated cylinder to determine its volume. A full write-up is available in Borgford and Summerlins Chemical Activities (Borgford, C. L.; Summerlin, L. R.; Chemical Activities, Teacher ed. American Chemical Society: Washington DC, 1988, pp 246247). Students can determine the amount of fat in potato chips and French fries by extracting the fat with hexane, as would be done with a Soxhlet extractor, using the procedure at  HYPERLINK "http://www.chymist.com/Fat in potato chips.pdf" http://www.chymist.com/Fat in potato chips.pdf. A McDonalds Happy Meal of hamburger or Chicken Nuggets, fries, and drink, is blended into a mush and then tested for the presence of protein (Biuret solution), sugar (Benedicts solution), starch (Lugols solution), vitamin C (indophenol solution), and sodium chloride (silver nitrate solution), using the procedure at  HYPERLINK "http://www.biologyjunction.com/mcmush_lab.htm" http://www.biologyjunction.com/mcmush_lab.htm. Out-of-class Activities and Projects (student research, class projects) Students can keep a record of all food and drink they consume, and any physical activity, for three days and compare their intake with that recommended at  HYPERLINK "http://www.choosemyplate.gov/" http://www.choosemyplate.gov/ or in the Tinnesand article. One example of this type of project is available at HYPERLINK "http://share.ehs.uen.org/node/2975"http://share.ehs.uen.org/node/2975. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  The ChemMatters Question from the Classroom column answers the question I heard someone say, You are what you eat. What is that supposed to mean? in the October 2000 issue. It discusses the idea that every atom in our bodies came from food/drink or air along with our bodies use of energy. (Becker, R. Question from the Classroom. ChemMatters, 2000, 18 (3), p 2) The article Hold the Meat!: Meat-Free Food Takes a Seat at the Table discusses four different types of meat-free proteins: tofu, tempeh, seitan, and Tofurky. It includes a discussion of why we need protein in our diets. (Nolte, B. Hold the Meat!: Meat-Free Food Takes a Seat at the Table. ChemMatters, 2011, 29 (4), pp 911) ____________________ This article from The Journal of Nutrition first discusses Atwaters role in the chemical analysis of food and moves on to later work in food composition research, including new analytic methods and collaborations between agencies. (Beecher, G. R.; Stewart, K. K.; Holden, J. M.; Harnly, J. M.; Wolf, W. R. Legacy of Wilbur O. Atwater: Human Nutrition Research Expansion at the USDAInteragency Development of Food Consumption Research. J. Nutr., 2009, 139 (1), pp 178184, DOI: 10.3945/jn.108.095547,  HYPERLINK "http://jn.nutrition.org/content/139/1/178.full.pdf+html" http://jn.nutrition.org/content/139/1/178.full.pdf+html) Wilbur O. Atwaters life and extensive work on food composition is described in this lecture. (Carpenter, K. J. The 1993 W. O. Atwater Centennial Memorial Lecture: The Life and Times of W. O. Atwater (18441907). J. Nutr., 1994, 124 (9), pp 1707S1714S,  HYPERLINK "http://jn.nutrition.org/content/124/9_Suppl/1707S.long" http://jn.nutrition.org/content/124/9_Suppl/1707S.long) A 2005 report from The Institute of Medicine of the National Academies, Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids offers an extensive resource on the bodys requirements to maintain health. ( HYPERLINK "http://www.nal.usda.gov/fnic/DRI/DRI_Energy/energy_full_report.pdf" http://www.nal.usda.gov/fnic/DRI/DRI_Energy/energy_full_report.pdf) The life and work of Johan Kjeldahl is summarized in a 1949 article from the Journal of Chemical Education. The full article is available to subscribers only, but the first page can be viewed at  HYPERLINK "http://pubs.acs.org/doi/abs/10.1021/ed026p459" http://pubs.acs.org/doi/abs/10.1021/ed026p459. (Veibel, S. Johann Kjeldahl (18491900) J. Chem. Educ., 1949, 26 (9), pp 459461) A discussion of the melamine contamination of food during 2007 and 2008 can be used to bring news reports into the chemistry classroom. The abstract can be seen at  HYPERLINK "http://pubs.acs.org/doi/abs/10.1021/ed800174h" http://pubs.acs.org/doi/abs/10.1021/ed800174h, while the full article is available only to subscribers. (Kimbrough, D. R.; Jensen, A. C. Using the Melamine Contamination of Foods to Enhance the Chemistry Classroom. J. Chem. Educ., 2010, 87 (5), pp 496499) A SourceBook module includes two experiments to determine the amount of various substances in food. (Hubert, J.; Miller, J. A.; Sherman, M. C. Food and Chemistry in SourceBook, Version 3.0, edited by Orna, M. V.; Smith, P. J. V. ChemSource, Inc.: New Rochelle, NY, 2010) Web sites for Additional Information (Web-based information sources) More sites on nutritional labeling An extensive 2010 report Examination of Front-of-Package Nutrition Rating Systems and Symbols: Phase I Report is available for download at The National Academies Press. It includes a detailed history of nutritional labeling and an analysis of current front-of-package labeling, with recommendations for improvements. ( HYPERLINK "http://www.nap.edu/catalog.php?record_id=12957" http://www.nap.edu/catalog.php?record_id=12957) The U.S. Food and Drug Administration Web site has a Food Labeling and Nutrition Overview page with links to information on rules regarding food labeling and other related information. ( HYPERLINK "http://www.fda.gov/Food/LabelingNutrition/default.htm" http://www.fda.gov/Food/LabelingNutrition/default.htm) The U.S. Department of Agriculture offers a search tool Whats in the Foods You Eat? at  HYPERLINK "http://www.ars.usda.gov/Services/docs.htm?docid=17032" http://www.ars.usda.gov/Services/docs.htm?docid=17032. The user can search nutrient profiles of 13,000 commonly eaten foods. The Chemical Heritage Foundation site includes several short biographical pieces on chemists who contributed to the knowledge of food chemistry and nutrition. ( HYPERLINK "http://www.chemheritage.org/discover/online-resources/chemistry-in-history/themes/public-and-environmental-health/food-chemistry-and-nutrition/index.aspx" http://www.chemheritage.org/discover/online-resources/chemistry-in-history/themes/public-and-environmental-health/food-chemistry-and-nutrition/index.aspx) More sites on calories Harvard Health Publications offers an extensive table showing calories burned per 30 minutes of various activities for people weighing 125, 155, and 185 pounds. It includes gym activities, sports, outdoor activities, daily life activities, occupational activities, and even home repair. ( HYPERLINK "http://www.health.harvard.edu/newsweek/Calories-burned-in-30-minutes-of-leisure-and-routine-activities.htm" http://www.health.harvard.edu/newsweek/Calories-burned-in-30-minutes-of-leisure-and-routine-activities.htm) The Mayo Clinic Web site has an online calorie calculator to estimate the number of calories needed daily to maintain your current weight. The user enters age, height, weight, gender, and activity level. ( HYPERLINK "http://www.mayoclinic.com/health/calorie-calculator/NU00598" http://www.mayoclinic.com/health/calorie-calculator/NU00598) The 1993 Agricultural Research article W.O. AtwaterFather of American Nutrition Science is reprinted on the U.S. Department of Agriculture Web site and summarizes Atwaters role in founding nutrition science in America and nutrition work done today. ( HYPERLINK "http://www.ars.usda.gov/is/timeline/nutrition.htm" http://www.ars.usda.gov/is/timeline/nutrition.htm) The most recent iteration of the food pyramid is a plate that shows suggested amounts of fruits, vegetables, grains, proteins, and dairy based on the area they take up on the plate. An entire U.S. Department of Agriculture site is dedicated to this guide to healthy eating. ( HYPERLINK "http://www.choosemyplate.gov/" http://www.choosemyplate.gov/) More sites on protein The Harvard School of Public Health has an online document that discusses how to make the best protein choices to eat, along with health issues associated with protein. ( HYPERLINK "http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/protein-full-story/index.html" http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/protein-full-story/index.html) Nutrition information on protein intake is provided online by the Centers for Disease Control and Prevention. ( HYPERLINK "http://www.cdc.gov/nutrition/everyone/basics/protein.html" http://www.cdc.gov/nutrition/everyone/basics/protein.html) The Exploratorium Science of Cooking Web site has a section on meat, including learning about what meat is and what gives meat its flavor and color, with meat-related experiments to try. ( HYPERLINK "http://www.exploratorium.edu/cooking/meat/index.html" http://www.exploratorium.edu/cooking/meat/index.html) Johan Kjeldahls work on determining the amount of nitrogen in an organic compound is described at  HYPERLINK "http://www.rsc.org/chemistryworld/Issues/2008/May/KjeldahlFlask.asp" http://www.rsc.org/chemistryworld/Issues/2008/May/KjeldahlFlask.asp. An extremely detailed guide on the chemistry of the Kjeldahl test and its history is offered at  HYPERLINK "http://www.buchi.ch/fileadmin/user_upload/02_applications/pdf/Buechi_Kjeldahl Guide.pdf" http://www.buchi.ch/fileadmin/user_upload/02_applications/pdf/Buechi_Kjeldahl Guide.pdf by Bchi Labortechnik. More sites on fats The Harvard School of Public Health discusses the idea that it is the type of fat you eat rather than the amount of calories from fat that is linked with disease and how to make healthy choices regarding fats. ( HYPERLINK "http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/fats-and-cholesterol/index.html" http://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/fats-and-cholesterol/index.html) The Soxhlet extraction is illustrated at  HYPERLINK "http://www.chem-ilp.net/labTechniques/SoxhletExtraction.htm" http://www.chem-ilp.net/labTechniques/SoxhletExtraction.htm using an animation and a short video. An Ask the Historian column from the Journal of Chemical Education describes the origin of the Soxhlet extractor. ( HYPERLINK "http://www.che.uc.edu/jensen/W. B. Jensen/Reprints/140. Soxhlet Ex..pdf" http://www.che.uc.edu/jensen/W. B. Jensen/Reprints/140. Soxhlet Ex..pdf) This page is designed more to be a promotion of a particular lab and its testing of fat content in foods, but it provides a good explanation of the benefits of using NMR for the testing. ( HYPERLINK "http://www.oxford-instruments.com/OxfordInstruments/media/industrial-analysis/magnetic-resonance-pdfs/Using-Nuclear-Magnetic-Resonance-to-Test-Fat-Content-in-Foods.pdf" http://www.oxford-instruments.com/OxfordInstruments/media/industrial-analysis/magnetic-resonance-pdfs/Using-Nuclear-Magnetic-Resonance-to-Test-Fat-Content-in-Foods.pdf) More sites on carbohydrates A Web site aimed at kids discusses simple and complex carbohydrates, along with how our body uses carbohydrates. ( HYPERLINK "http://kidshealth.org/kid/stay_healthy/food/carb.html" http://kidshealth.org/kid/stay_healthy/food/carb.html) The WebMD Web site discusses good carbs and bad carbs and making healthy nutrition choices. ( HYPERLINK "http://www.webmd.com/food-recipes/features/carbohydrates?page=2" http://www.webmd.com/food-recipes/features/carbohydrates?page=2) The University of Sydney offers a searchable database of glycemic index values for various foods at  HYPERLINK "http://www.glycemicindex.com/" http://www.glycemicindex.com/. The Food and Agriculture Organization of the United Nations discusses methods for analyzing foods for fats, proteins, and carbohydrates. ( HYPERLINK "http://www.fao.org/docrep/006/Y5022E/y5022e03.htm" http://www.fao.org/docrep/006/Y5022E/y5022e03.htm) More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers) The U.S. Food and Drug Administration Web site has a collection of materials for using and promoting nutrition facts labels. For example, the section Understanding and Using the Nutrition Facts Label has nutrition facts label images to download, information on how to read the label, how to choose healthy foods using the label, and related videos. ( HYPERLINK "http://www.fda.gov/Food/ResourcesForYou/Consumers/NFLPM/default.htm" http://www.fda.gov/Food/ResourcesForYou/Consumers/NFLPM/default.htm) The Institute of Food Technologists offers a 63-page pdf booklet Food Chemistry Experiments at  HYPERLINK "http://www.accessexcellence.org/AE/AEPC/IFT/pdfs/IFTFoodExperiments.pdf" http://www.accessexcellence.org/AE/AEPC/IFT/pdfs/IFTFoodExperiments.pdf. It contains units on carbohydrates, lipids, and proteins, each with a student experiment, extensive chemistry background information, and related puzzles. Two Is Better than One Background Information (teacher information) More on Maillard reactions The Maillard reaction is referred to as a non-enzymatic browning process, distinguishing it from the common browning reaction seen in cut fruits and vegetables, which is driven by enzymes such as polyphenol oxidase and catechol oxidase. The Maillard reaction contributes to the flavor and/or color of many foods, including bread, coffee, chocolate, meat, and beer. It can also contribute to unwanted reaction products, such as causing off-flavor, poor color, and loss of nutritional value of food products, such as in stored foods. ( HYPERLINK "http://jn.nutrition.org/content/105/8/989.full.pdf" http://jn.nutrition.org/content/105/8/989.full.pdf) It also relates to medical issues, such as cataract formation and diabetes. An October 1, 2012 article in Chemical & Engineering News focused on the 100th birthday of the Maillard reaction. Although the majority of the reactions users probably have never heard the term Maillard reaction, a quote from the article comments on just how common this reaction is: The Maillard is, by far, the most widely practiced chemical reaction in the world, said chemistry Nobel Prize winner Jean-Marie Lehn late last month in Nancy, France, some 20 miles from the village of Pont--Mousson, where Maillard was born. Thats because the reaction takes place daily in households around the globe whenever food is cooked, Lehn told the group of 270 international scientists who had gathered on Maillards home turf to honor the reactions centennial and attend this years International Maillard Reaction Society conference. (p 58) The reaction was first reported in 1912 by Louis-Camille Maillard, a French chemist. Afterward, its study could be said to go through a rest period, before interest in it was renewed: Yet even with the simplest of reactants, Maillard chemistry was so complicated and produced so many productshundreds of themthat the research world would largely ignore it until around the time of World War II, Rocke [a historian at Case Western Reserve University] said. Thats when the military became interested in producing on an industrial scale food that both was palatable and had a long shelf life. Because the Maillard reaction is responsible for the appealing aromas of freshly cooked food as well as some of the unwelcome ingredients in processed or long-stored food, scientists began to seriously study the reaction, Rocke explained. Then in 1953, an African American chemist named John E. Hodge, who worked at the U.S. Department of Agriculture in Peoria, Ill., published a paper that established a mechanism for the Maillard reaction (J. Agric. Food Chem. 1953, 1, 928). According to Hodges model, the Maillard reaction has three stages. First, the carbonyl group of a sugar reacts with an amino group on a protein or amino acid to produce water and an unstable glycosylamine. Then, the glycosylamine undergoes Amadori rearrangements to produce a series of aminoketose compounds. Last, a multitude of molecules, including some with flavor, aroma, and color, are created when the aminoketose compounds undergo a host of further rearrangements, conversions, additions, and polymerizations. (p 58) (Everts, S. Chem. Eng. News, 2012, 90 (40), pp 5860;  HYPERLINK "http://cen.acs.org/articles/90/i40/Maillard-Reaction-Turns-100.html" http://cen.acs.org/articles/90/i40/Maillard-Reaction-Turns-100.html) Part of the driving force behind the renewed interest in the Maillard reaction came from the military, because of difficulties with storing foods for long-term: World War II soldiers were complaining about their powdered eggs turning brown and developing unappealing flavors. After many studies done in laboratories, scientists figured out that the unappetizing tastes were coming from the browning reaction. Even though the eggs were stored at room temperature, the concentration of amino acids and sugars in the dehydrated mix was high enough to produce a reaction. Most of the research done in the 1940s and 1950s centered on preventing this reaction. ( HYPERLINK "http://www.exploratorium.edu/cooking/meat/INT-what-makes-flavor.html" http://www.exploratorium.edu/cooking/meat/INT-what-makes-flavor.html) The Ioana Urma figure shown in the Husband article is an interesting simplification of the reaction process. The figure was produced by Urma, an artist/designer/architect, for the television show Top Chef Masters, season 3, episode 8, Blinded Me with Science. ( HYPERLINK "http://www.bravotv.com/top-chef-masters/season-3/blinded-me-with-science" http://www.bravotv.com/top-chef-masters/season-3/blinded-me-with-science) Contestant chefs had to demonstrate a particular science concept, one of which was the Maillard reaction, to judges. The figure used in the article is actually an intermediate-stage design; the final design used on the show is shown below. Her description of the process she went through to learn enough about the reaction to effectively communicate it on a large poster on television is intriguing, one of a non-chemist deciding how to best share information on a complex topic. ( HYPERLINK "http://blog.ioanacolor.com/2011/06/maillard-reaction/" http://blog.ioanacolor.com/2011/06/maillard-reaction/)   HYPERLINK "http://blog.ioanacolor.com/wp-content/uploads/2011/06/ioana_top-chef-masters_science_maillard-reaction.jpg" http://blog.ioanacolor.com/wp-content/uploads/2011/06/ioana_top-chef-masters_science_maillard-reaction.jpg The Maillard reaction has an ideal temperature range (as seen in the figure above), 250300 F/110149 C, where the reaction can proceed quickly, as needed during cooking foods for immediate consumption. One can see a crossover point after food passes 212 F/100 C, the boiling point of water. At least some water is required for the reaction, but as Husband states regarding toast, much of the water must evaporate first for an optimum product. It is a balancing act of too much water vs. not enough: The presence of water limits the maximum attainable temperature as it boils off from the surface of foods, thereby slowing the Maillard reaction. However, once water has evaporated, for example, in a bread crust or on the surface of a french fry, the drier surface allows the temperature to exceed 212F (100C), which in turn drastically speeds up the Maillard reaction. Similarly, a piece of toast browns in the outermost layer only. But less water is not always better. There is an optimum water level required for the Maillard reaction to proceed. If the food gets too dry, the lack of water will actually slow down the Maillard reaction as the mobility of the reagents decreases. This also contributes to the difficulty of producing a food that is nicely browned while cooking in a microwave: Microwavable pies with browning crusts are challenging to produce because microwaves primarily interact with water and therefore bring the temperature only up to the boiling point. This is the reason microwave cooking in general does not contribute much flavor to dishes and why microwave ovens are used mainly to reheat food. In order to get a nice browning of a pie crust in a microwave, pH adjustment is combined with the addition of reducing sugars and amino acids. The reaction can also proceed at much lower temperatures, but much more slowly. Champagne is one example: Contrary to popular belief the Maillard reaction will also occur at lower temperatures. In vintage Champagne, autolyzed (inactive) yeast and sugars react to form Maillard products that yield a characteristic flavor profile. This reaction takes place in the cool chalk cellars of the Champagne district in France, where the temperature remains constant at 48 to 54F (912C) year round. Because of the low temperature, a much longer reaction time is needed, so the characteristic Maillard-influenced flavor is found only in aged Champagnes. (from  HYPERLINK "http://blog.khymos.org/2012/06/04/maximizing-food-flavor-by-speeding-up-the-maillard-reaction/" http://blog.khymos.org/2012/06/04/maximizing-food-flavor-by-speeding-up-the-maillard-reaction/) Controlling the reaction to provide desired results and to reduce the occurrence of undesired reaction products are high on the priority list for those involved in food chemistry: Over the past several decades, theres been a huge effort by food scientists to figure out how to influence the end products, Fogliano [a food chemist at the University of Naples, Federico II] said. Theyve looked at various starting sugars and proteins as well as how different temperatures, pH levels, moisture levels, and other ingredients affect the creation of desired and undesired odor and flavor products. The idea, he added, is to figure out how to control the unruly Maillard process as it happens in food. For example, Hofmann [the chair of food chemistry and molecular sensory science at Technical University of Munich] said, its primarily the amino acid that drives the odor quality, not the sugar. Glycine reactions produce beerlike odors, valine reactions produce characteristic rye-bread smells, and cysteine is the amino acid responsible for many meat and cracker scents, he said. Maillard reactions can also change the texture and consistency of food, said HYPERLINK "http://www.chm.tu-dresden.de/lc1/"Thomas Henle, a food chemist at Dresden University of Technology. For example, the Maillard reaction is used to append sugars to the protein lactalbumin, which is then used to make yogurt more gelatinous. Meanwhile, adding sugar to a protein called -lactoglobulin in processed cheese makes the product softer and creamier, he said. Sometimes a Maillard product that is appealing in some processed foods is undesirable in others. Case in point: 2-acetyl-1-pyrroline. This molecule gives crusty bread, popcorn, and basmati rice a desirable odor and flavor, but its presence in ultra-high-temperature pasteurized milk, because of the processing, results in an off-putting aftertaste that many consumers dislike, Hofmann says. More notorious outcomes of the Maillard reaction in food are 5-hydroxymethylfurfural (HMF) and acrylamide, both potential carcinogens. Ten years ago, Stockholm University food chemists Margareta Trnqvist and Eden Tareke published a paper that sent shock waves through the food regulatory and science community: They showed that heavily processed food such as french fries, chips, and biscuits contained milligram levels of acrylamide (J. Agric. Food Chem.,  HYPERLINK "http://pubs.acs.org/doi/abs/10.1021/jf020302f" http://pubs.acs.org/doi/abs/10.1021/jf020302f). (p 59) (from (Everts, S. Chem. Eng. News 2012, 90 (40), pp 5860;  HYPERLINK "http://cen.acs.org/articles/90/i40/Maillard-Reaction-Turns-100.html" http://cen.acs.org/articles/90/i40/Maillard-Reaction-Turns-100.html) The Husband article focuses on Maillard reactions that occur during cooking. However, the reactions can play a role in situations related to the human body. Two medical conditions related to the Maillard reaction are cataract formation and diabetes: One reaction hot spot is the lens of the human eye, where Maillard-based chemistry is partly responsible for nuclear cataracts. In this prevalent form of the disease, the cataracts darken and need to be extracted, he said. Because lens cells dont regenerate over a lifetime and they have high levels of ascorbic acid, which can enhance Maillard reactions, the lens is a trash can for human Maillard reactions, he added. Diabetes is another major area of medical Maillard research. The increased levels of sugar in the bloodstream result in Maillard reactions that activate the bodys inflammation response and contribute to many of the liver and cardiovascular complications of the disease, Monnier said. In fact, the human body has several endogenous systems in place to remove these Maillard reaction products, said HYPERLINK "http://www2.warwick.ac.uk/fac/med/staff/thornalley/"Paul Thornalley, a researcher at Englands Warwick Medical School. Thornalley studies enzymes that our body produces to eliminate methylglyoxal, a common Maillard reaction product circulating in our bloodstream. Left unchecked, methylglyoxal wreaks all sorts of damage, including interfering with cell surface proteins needed to keep blood vessel cells attached to each other. Although the enzymes responsible for breaking down methylglyoxal work 99.7% of the time, some methylglyoxal still slips under the fence and does damage, particularly in diabetics, he said. (p 60) (from (Everts, S. Chem. Eng. News, 2012, 90 (40), pp 5860;  HYPERLINK "http://cen.acs.org/articles/90/i40/Maillard-Reaction-Turns-100.html" http://cen.acs.org/articles/90/i40/Maillard-Reaction-Turns-100.html) Students may be surprised to learn that the Maillard reactions ability to give their steak a pleasing brown color also plays a role in browning human skin using self-tanning products. The active ingredient in self-tanners is dihydroxyacetone, abbreviated DHA, which is a sugar that is able to react with amino acids from skin proteins. The history of its discovery and a description of its action were the focus of a Chemical & Engineering News Whats That Stuff? column: DHA's browning effects were discovered by accident. In the mid-1950s, at Children's Hospital at the University of Cincinnati, researcher Eva Wittgenstein was studying the effect of large oral doses of DHA in children who had glycogen storage disease. These kids were ingesting a lot of DHAas much as 1 g per kg of their body weight. Sometimes the children spit up some of this sweet concentrated material, and it splashed on their skin. A few hours later, the kids had brown spots on their skin where stray splashes hadn't been wiped off. Wittgenstein was able to do something with her observations other than berating the staff for not getting those kids cleaned up. Curious, she prepared aqueous solutions of varying concentrations of DHA and was able to reproduce the pigmentation on her own skin. The reaction of skin with DHA to produce an artificial tan proceeds through combination with free amino groups in skin proteins, and particularly by combination of DHA with the free guanido group in arginine. (Epidermal proteins have a very high content of the amino acids arginine, lysine, and histidine.) In related experiments, Wittgenstein found that arginine was the most reactive, with the appearance of a dark brown color within 30 minutes. Aqueous mixtures of DHA with glycine, lysine, and histidine also gave brown to yellow pigments. These pigments are called melanoidins. Melanoidins are polymeric compounds that are linked by lysine side chains to the proteins of the stratum corneumwhich is the outermost, dead layer of human skin. And DHA doesn't penetrate any further than the stratum corneum. Wittgenstein figured this out by tape stripping. After she treated her skin with a solution of DHA and before the pigment had developed, she put some tape on her skin and pulled it off. No pigment formed in the peeled area. (p 46) (Chem. Eng. News, 2000, 78 (24), p 46;  HYPERLINK "http://pubs.acs.org/cen/whatstuff/stuff/7824scit2.html" http://pubs.acs.org/cen/whatstuff/stuff/7824scit2.html) A 2003 paper The Shroud of Turin: An amino-carbonyl reaction (Maillard reaction) may explain the image formation reports on the Maillard reactions potential relation to the facial pattern seen on the Shroud of Turin. The summary of the paper states: The Shroud of Turin is a large piece of linen that shows the faint image of a man on its surface: it has been claimed to be the shroud of Jesus. Here we report evidences that colour can be produced by reactions between reducing sugars, left on the cloth by the manufacturing procedure, and amines deriving from the decomposition of a corpse. Treatment of a cloth prepared according to the ancient technology gave a distribution of colour on the thread fibres in good agreement with the Shroud features. Such a natural image-production process would support the hypothesis that the Shroud of Turin had been a real shroud. However, these observations do not prove how the image was formed or the "authenticity" of the Shroud. ( HYPERLINK "https://shroud.com/pdfs/rogers7.pdf" https://shroud.com/pdfs/rogers7.pdf) More on bread and toast Egyptians are often credited with being the first to discover a way of producing leavened loaves of bread. As a method of food preservation back then, bread slices could be toasted. The word toast comes from the Latin phrase tostum, meaning to scorch or burn. ( HYPERLINK "http://toaster-review.toptenreviews.com/the-toaster-and-its-origins-a-toast-timeline.html" http://toaster-review.toptenreviews.com/the-toaster-and-its-origins-a-toast-timeline.html) The Husband article describes two substances in flour: starch, a carbohydrate, and gluten, a protein. Carbohydrates are sometimes referred to as sugars or saccharides. Common monosaccharides, or single units of sugars, are glucose and fructose. Common disaccharides, or two sugar units bonded together, are sucrose and lactose. Polysaccharides contain many repeating sugar units bonded together. Starch is classified as a polysaccharide. One of the figures in the Husband article shows a representation of a starch molecule containing two types of starch molecules, amylose and amylopectin. Both are composed of many of the single sugar units, glucose; however, the structures of the two are quite different. An amylose chain is linear, while an amylopectin chain has branching points. Starch is the major component of flour produced from wheat: Wheat flour contains 7073% starch and most commonly anywhere between 814.5% protein. If you look at flour under a microscope you can see lots of brick like structures called cells. In each cell you will see a granule of starch surrounded by glassy looking protein. Different types of starch have different structures. Potato starch is oval in shape, wheat starch is oval or round but smaller than potato starch, and maize starch has a "rocky" look. Starch is a storage carbohydrate of plants such as cereals (wheat, maize, oats, rice and barley), tubers (potatoes, cassava and taro) and pulses (peas and beans). In whole wheat grains it makes up 6070% of the grain. It is found in the endosperm which is the part of the grain that white flour is milled from. ( HYPERLINK "http://www.bakeinfo.co.nz/Facts/Bread-making/Bread-ingredients/Starch" http://www.bakeinfo.co.nz/Facts/Bread-making/Bread-ingredients/Starch) Gluten is also found in the endosperm and is part of flour, making up the protein component. Harold McGee describes gluten and its chemical interactions in On Food and Cooking: The Science and Lore of the Kitchen: Gluten is a complex mixture of certain wheat proteins that cant dissolve in water, but do form associations with water molecules and with each other. When the proteins are dry, theyre immobile and inert. When wetted with water, they can change their shape, move relative to each other, and form and break bonds with each other. ... Most of the gluten proteins, the gliadins and the glutenins, are around a thousand amino acids long. The gliadin chains fold onto themselves in a compact mass, and bond only weakly with each other and with the glutenin proteins. The glutenins, however, bond with each other in several ways to form an extensive, tightly knit network. At each end of the glutenin chain are sulfur-containing amino acids that can form strong sulfursulfur bonds with the same amino acids at the ends of other glutenin chains. The long, coiled middle stretch of the glutenin molecule consists mainly of amino acids that form weaker, temporary bonds (hydrogen and hydrophobic bonds) with similar amino acids. Glutenin chains thus link up with each other end-to-end to form super-chains a few hundred glutenins long, and coiled stretches along their lengths readily form many temporary bonds with similar stretches along neighboring gluten proteins. The result is an extensive inter-connected network of coiled proteins, the gluten. (pp 521522) One of the student activities suggested in the section In-class Activities is to isolate the gluten in flour. McGee describes why this is simple to do and mentions glutens use in making vegetarian products: Because theyre both cohesive and insoluble in water, the gluten proteins are easily separated from the rest of the flour: you simply make a dough, then knead it in water. The starch and water-soluble substances wash away, and tough, chewy gluten remains. Gluten as a unique food ingredient was discovered by Chinese noodle makers around the 6th century, and by the 11th was known as mien chin, or the muscle of flour. One of the simplest ways to prepare gluten is to pinch off small bits and deep-fry them; they puff up into light chewy balls that readily absorb the flavor from a sauce. Today gluten is widely available and used to make a variety of vegetarian meats. (p 468) During breadmaking, starch, gluten, and water interact. The process and its purpose is briefly described: The main function of gluten in dough during mixing is to swell and hold water, forming cells with strong, elastic walls which capture the carbon dioxide formed during fermentation. The starch granules do not swell at fermentation temperatures. However, at the higher temperatures of the oven the starch does swell to form a jelly structure and for this it needs water which it steals from the swollen gluten. At the same time as the gluten is losing part of its water it is setting, due to the heat, so that in the final bread we have two complete structures, one of starch and one of protein. Either of these can be dissolved away without the bread losing its shape. ( HYPERLINK "http://nzic.org.nz/ChemProcesses/food/6C.pdf" http://nzic.org.nz/ChemProcesses/food/6C.pdf) Starch also plays a role in the staling of bread. Initially the linear amylose polymers in flour about one fifth of the total starch are aligned, side by side, in a microcrystalline structure. This structure is destroyed during baking, but subsequently crystallization recommences, to a crystal form which contains lots of water of hydration. This reduces the amount of free water in the bread, and it loses springiness and appears to dry out. ( HYPERLINK "http://www.rsc.org/chemistryworld/Issues/2009/October/Ontherise.asp" http://www.rsc.org/chemistryworld/Issues/2009/October/Ontherise.asp) More on potatoes and French fries Potatoes are also made up of a high percentage of starch. As mentioned in the previous section, even though starches have the same molecules, amylose and amylopectin, different types of starch granules have different appearances, comparing potato, wheat, and maize starches. McGee describes potato starch in On Food and Cooking: The Science and Lore of the Kitchen: Compared to the starches from dry grains, the starches from moist underground storage organs [e.g. potatoes] come in the form of larger granules that retain more water molecules, cook faster, and release starch at lower temperatures. They contain less amylose, but their amylose chains are up to four times longer than cereal amyloses. Root and tuber starches contain a fraction of the lipids and proteins that are associated with cereal starches, which makes them more readily gelatedlipids delay gelation by stabilizing granule structureand gives them less pronounced flavors. Potato starch was the first commercially important refined starch and is still an important food starch in Europe. It is unusual for several characteristics. Its granules are very large, up to a tenth of a millimeter across, and its amylose molecules are very long. (pp 614615) McGee also briefly describes the history of the term French fry and mentions the double-frying technique. Fried potatoes are some of the worlds favorite foods. Deep-fried potato sticks and slices and the technique of double-frying were all well known in Europe by the middle of the 19th century, and in England were attributed mainly to the French: hence the term French fry for what the French simply call fried potatoes (pommes frites). French fries may first have been made in significant quantities by Parisian street vendors early in the 19th century. They are potato sticks cut with a square cross section, 510 mm on a side, deep-fried in oil, with a crisp gold exterior and a moist interior thats fluffy if the potatoes are high-starch russets, creamy otherwise. Simple quick frying doesnt work very well; it gives a thin, delicate crust thats quickly softened by the interiors moisture. A crisp crust requires an initial period of gentle frying, so that starch in the surface cells has time to dissolve from the granules and reinforce and glue together the outer cell walls into a thicker, more robust layer. (pp 303304) Part of food research revolves around potentially dangerous compounds that can develop when food is processed and how the compounds can best be minimized. For example, an earlier section mentions the formation of acrylamide. A 2005 Journal of Chemical Education article discusses research done with potatoes exploring ways that acrylamide can be better controlled: Acrylamide is a human neurotoxin, known rodent carcinogen and probable human carcinogen. In the four years since its identification in food, a desire to reduce its levels in food has developed due to associated health risks. Several reports indicate that asparagines and sugars are the precursors of the acrylamide in food, which forms through the Maillard reaction. Eliminating acrylamide formation in foods by stopping the Maillard reaction is not completely desirable, since it produces desirable taste and aroma in foods. A Canadian research team led by Adam Becalski recently identified the relationship between possible acrylamide precursors, namely sugars and amino acids, present in food and the level of acrylamide produced. The investigators used French fries as the food item for the investigation. Different levels of sugars and amino acids in the raw potatoes and different processing methods were suggested as the source of the variance in acrylamide levels. Additional research suggests that cold storage temperatures enhance acrylamide levels. While commercial production of French fries might be limited by the variety of potato cultivars available on a commercial scale, this work clearly demonstrates that if a variety low in sugar is selected, acrylamide levels can be significantly reduced when using a standard industry cooking method. (p 12). (King, A.G. J. Chem. Educ., 2005, 82 (1), pp 1014) More on meat Although its composition is drastically different from the starch-rich bread and potatoes described earlier in the Husband article, meat also undergoes the Maillard reaction. McGee discusses the basics of meat in On Food and Cooking: The Science and Lore of the Kitchen: Lean meat is made up of three basic materials: its about 75% water, 20% protein, and 3% fat. These materials are woven into three kinds of tissue. The main tissue is the mass of muscle cells, the long fibers that cause movement when they contract and relax. Surrounding the muscle fibers is the connective tissue, a kind of living glue that harnesses the fibers together and to the bones that they move. And interspersed among the fibers and connective tissue are groups of fat cells, which store fat as a source of energy for the muscle fibers. The qualities of meatits texture, color, and flavorare determined to a large extent by the arrangement and relative proportions of the muscle fibers, connective tissue, and fat tissue. The major connective-tissue filament is the protein called collagen, which makes up about a third of all the protein in the animal body, and is concentrated in skin, tendons, and bones. The name comes from the Greek for glue producing, because when its heated in water, solid, tough collagen partly dissolves into sticky gelatin. So unlike muscle fibers, which become tougher with cooking, the connective tissue becomes softer. An animal starts out life with a large amount of collagen thats easily dissolved into gelatin. As it grows and its muscles work, its total collagen supply declines, but the filaments that remain are more highly crosslinked and less soluble in hot water. This is why cooked veal seems gelatinous and tender, mature beef less gelatinous and tougher. (pp 129130) McGee also describes the results of cooking meat: If fresh meat never gets hotter than the boiling point of water, then its flavor is largely determined by the breakdown products of proteins and fats. However, roasted, broiled, and fried meats develop a crust that is much more intensely flavored, because the meat surface dries out and gets hot enough to trigger the Maillard or browning reactions. Meat aromas generated in the browning reactions are generally small rings of carbon atoms with additions of nitrogen, oxygen, and sulfur. Many of these have a generic roasted character, but some are grassy, floral, oniony or spicy, and earthy. Several hundred aromatic compounds have been found in roasted meats! (p 148) The aging of meat also plays a role in the Maillard reaction and the softening of the collagen connective tissue. When meat is aged, enzymes in the muscles work to break down molecules in the meat, such as breaking down proteins into amino acids, and glycogen, which is a polysaccharide used to store energy in the body, into glucose. These can eventually react in the Maillard reaction when the meat is cooked. In addition, enzymes also help to break down the collagen. Connections to Chemistry Concepts (for correlation to course curriculum) Physical/chemical changesThe double cooking techniques described in the article can be separated into physical and chemical changes; for example, water evaporates from the surface of the bread, a physical change, before undergoing the Maillard reaction to brown the bread, a chemical change. Additional examples beyond the article could be used; the In-class Activities section below has a link to an activity Physical and Chemical Changes in Food. Phase changesThe different types of phase changes involved in preparing food can be discussedat a minimum, the evaporation of water from bread. BiochemistryThe foods mentioned in the article all contain carbohydrates and proteins, examples of commonly discussed biochemical molecules. Possible Student Misconceptions (to aid teacher in addressing misconceptions) The Maillard reaction is a single reaction. The Maillard reaction is actually a complex series of reactions, with a huge number of potential products depending on the reducing sugar(s) and amino acid(s) that participate in the process. The Maillard reaction is totally understood. Even after a hundred years of study, the complex chain of reactions and products involved in the Maillard reaction is not totally understood and continues to be studied. The Maillard reaction only relates to food. Maillard reactions occur not only in food, but are also part of processes related to the human body, such as cataract formation, diabetes, and even the use of self-tanners on the skin. Anticipating Student Questions (answers to questions students might ask in class) Are Maillard reactions the only way that food can brown? There are several types of browning reactions: Maillard, caramelization, ascorbic acid oxidation, and phenolase browning. The first three are non-enzymatic, while the fourth involves enzyme action. Students may be most familiar with phenolase browning, which can occur on the cut surface of fruits and vegetables. ( HYPERLINK "http://www.landfood.ubc.ca/courses/fnh/301/brown/brown_prin.htm" http://www.landfood.ubc.ca/courses/fnh/301/brown/brown_prin.htm) In-class Activities (lesson ideas, including labs & demonstrations) An activity at the Exploratorium Web site allows students to smell the Maillard reaction. They mix a small amount of corn syrup with an amino acid caplet from a health-food store, heat it on high, and make observations of odor and color. A full description is available at  HYPERLINK "http://www.exploratorium.edu/cooking/meat/activity-maillard.html" http://www.exploratorium.edu/cooking/meat/activity-maillard.html. A blog post at  HYPERLINK "http://www.the-science-mom.com/296/the-chemistry-of-toast-a-food-science-investigation/" http://www.the-science-mom.com/296/the-chemistry-of-toast-a-food-science-investigation/ describes a simple experiment using a piece of toast and iodine. The toast is cut so the interior is exposed. An iodine solution is applied to the exterior and interior and observed. Students can isolate and compare the amount of gluten in different types of flour using the activity at  HYPERLINK "http://www.exploratorium.edu/cooking/bread/activity-gluten.html" http://www.exploratorium.edu/cooking/bread/activity-gluten.html. They mix flour and water, knead it into dough, let it sit for ten minutes, then run cold water over the dough ball to wash away the other dough components, such as starch, leaving the gluten behind. Students could try a collagen experiment using stew meat. Beef is cooked in a slow cooker, with a few pieces removed after 30 minutes of cooking, and additional pieces after six hours of cooking. Both are then tasted and compared. ( HYPERLINK "http://static.cookingforgeeks.com/press/press-kits/cooking-for-geeks-p194-collagen-experiment.pdf" http://static.cookingforgeeks.com/press/press-kits/cooking-for-geeks-p194-collagen-experiment.pdf) The activity Physical and Chemical Changes in Food describes ten stations for students to visit to classify the change in food. For example, making toast, letting a chocolate Hersheys Kiss sit in your mouth to melt, drinking a flat sample of soda vs. a fresh sample and testing the pH of both, etc. Since tasting is involved, it would probably be better to do this activity outside the laboratory setting. ( HYPERLINK "http://apps.caes.uga.edu/sbof/main/lessonPlan/physChemChangeInFood.pdf" http://apps.caes.uga.edu/sbof/main/lessonPlan/physChemChangeInFood.pdf) Out-of-class Activities and Projects (student research, class projects) Students can head to the kitchen and try the food examples described in the article for themselves. For schools with family and consumer science courses, teachers could collaborate to combine chemistry and cooking. Students, particularly those who have part-time jobs working at a fast food restaurant, could research and compare the methods different restaurants use for making French fries, along with relevant taste tests. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  The ChemMatters article Attack of the Gluten describes glutens presence in grain and its role in breadmaking and discusses gluten sensitivity. It also includes a brief dough-making activity. (Hill, M. ChemMatters, 2012, 30 (1), pp 911) ________________ McGees book is a definitive work on the science of cooking, made readable and understandable for the non-chemist as well as the non-cook. It includes sections related to the Husband article on bread, potatoes, meat, and the Maillard reaction. (McGee, H. On Food and Cooking: The Science and Lore of the Kitchen; Scribner: New York, 2004.) Angela Kings Research Advances column in the Journal of Chemical Education briefly highlights current research. A section in a 2005 column focuses on food research on acrylamide in French fries. (King, A.G. J. Chem. Educ., 2005, 82 (1), pp 1014) Web sites for Additional Information (Web-based information sources) More sites on Maillard reactions The process and known pathways of the Maillard reaction series are discussed here: ( HYPERLINK "http://www.food-info.net/uk/colour/maillard.htm" http://www.food-info.net/uk/colour/maillard.htm). The November 21, 2011 issue of Chemical & Engineering News has the results of an invitation to bloggers to write about their favorite chemical reaction. Matthew Hartingss favorite is the Maillard reaction. ( HYPERLINK "http://cen.acs.org/articles/89/i47/Maillard-Reaction.html" http://cen.acs.org/articles/89/i47/Maillard-Reaction.html) A blog post by a Ph.D. chemist discusses ways that the Maillard reaction can be either sped up or slowed down, to achieve desired results while cooking or storing foods. ( HYPERLINK "http://blog.khymos.org/2012/06/04/maximizing-food-flavor-by-speeding-up-the-maillard-reaction/" http://blog.khymos.org/2012/06/04/maximizing-food-flavor-by-speeding-up-the-maillard-reaction/) Although there are no lectures that appear to be specific to the Maillard reaction, an excellent free series of lectures is available in fall 2012, with the lectures streamed live and archived on YouTube and iTunes. The lectures are a collaboration between eminent Harvard researchers and world-class chefs. ( HYPERLINK "http://www.seas.harvard.edu/cooking" http://www.seas.harvard.edu/cooking) More sites on bread and toast The Exploratorium Science of Cooking Web site has a section dedicated to the science of bread: ( HYPERLINK "http://www.exploratorium.edu/cooking/bread/index.html" http://www.exploratorium.edu/cooking/bread/index.html). A site on breadmaking includes this separate page describing one of the key ingredients of bread, starch. ( HYPERLINK "http://www.bakeinfo.co.nz/Facts/Bread-making/Bread-ingredients/Starch" http://www.bakeinfo.co.nz/Facts/Bread-making/Bread-ingredients/Starch) Toast is serious business for food researcher Dom Lane. The Newscripts column in Chemical & Engineering News describes his research on the perfect piece of toast. ( HYPERLINK "http://cen.acs.org/articles/89/i33/Perfect-Toast-Tonic-Skin-Titanium.html" http://cen.acs.org/articles/89/i33/Perfect-Toast-Tonic-Skin-Titanium.html) An October 2009 Royal Society of Chemistry article On the Rise discusses the chemistry of making bread. ( HYPERLINK "http://www.rsc.org/images/BreadChemistry_tcm18-163980.pdf" http://www.rsc.org/images/BreadChemistry_tcm18-163980.pdf) A chapter in The Wonder Book of Chemistry titled The Slice of Toast uses an overtoasted slice of bread as an example of carbon, as the main character, Uncle Paul, speaks to his nephews about the elements. ( HYPERLINK "http://www.mainlesson.com/display.php?author=fabre&book=chemistry&story=toast" http://www.mainlesson.com/display.php?author=fabre&book=chemistry&story=toast) More sites on potatoes and French fries A Popular Mechanics post discusses the science of frying and how one can produce The Perfect French Fry. ( HYPERLINK "http://www.popularmechanics.com/science/how-to-make-the-perfect-french-fry" http://www.popularmechanics.com/science/how-to-make-the-perfect-french-fry) The December 2005 Food Chemistry article Structural changes of potato tissue during French fries production is only available to subscribers, but the articles abstract page shows the articles figures, which include a series of scanning electron microscope images of the potato tissue and its changes. ( HYPERLINK "http://www.sciencedirect.com/science/article/pii/S0308814604008015" http://www.sciencedirect.com/science/article/pii/S0308814604008015) Acrylamide formation during the cooking of French fries has been a concern; a summary of a 2011 Journal of Agricultural and Food Chemistry article discusses possible ways acrylamide levels can be minimized and their effectiveness. ( HYPERLINK "http://www.foodnavigator.com/Science-Nutrition/Study-shows-limitations-and-potential-of-Acrylamide-Toolbox" http://www.foodnavigator.com/Science-Nutrition/Study-shows-limitations-and-potential-of-Acrylamide-Toolbox) An American Chemical Society press release highlights an August 2012 article from Journal of Agricultural and Food Chemistry that describes potential ways to reduce levels of acrylamide while cooking French fries. ( HYPERLINK "http://portal.acs.org/portal/PublicWebSite/pressroom/presspacs/CNBP_030745" http://portal.acs.org/portal/PublicWebSite/pressroom/presspacs/CNBP_030745) More sites on meat An American Chemical Society undergraduate blog discusses the chemistry of barbecue, including a discussion of the Maillard reaction and a link to a Bytesize Science video on barbecue. ( HYPERLINK "http://acsundergrad.wordpress.com/2012/08/10/the-chemistry-of-bbq/" http://acsundergrad.wordpress.com/2012/08/10/the-chemistry-of-bbq/) The Chemistry of Beef Flavor is a pdf document prepared for the National Cattlemens Beef Association and summarizes the effect various factors, such as breed of beef, the diet cattle eat, aging, etc., can have on the flavor of cooked meats. ( HYPERLINK "http://www.beefresearch.org/CMDocs/BeefResearch/The Chemistry of Beef Flavor.pdf" http://www.beefresearch.org/CMDocs/BeefResearch/The Chemistry of Beef Flavor.pdf) A food service guide to beef is interesting, in that it shares information about the science of meat along with tips about proper cooking. ( HYPERLINK "http://www.ginsbergs.com/wp-content/uploads/2012/06/04_BeefCookery.pdf" http://www.ginsbergs.com/wp-content/uploads/2012/06/04_BeefCookery.pdf) More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers) A study module for a food chemistry course is designed for the undergraduate level, but includes much useful information on water, carbohydrates, proteins, lipids, and browning reactions. ( HYPERLINK "http://www.landfood.ubc.ca/courses/fnh/301/brown/brown_prin.htm" http://www.landfood.ubc.ca/courses/fnh/301/brown/brown_prin.htm) . Whats That Smell? Background Information (teacher information) More on the history of perfumes and fragrances It is thought that perfumes and fragrances date back thousands of years. The word perfume comes from the Latin per fume, translated as through smoke. The oldest of perfumes comes from the burning of incense and aromatic herbs used in religious services. Some of the substances used include aromatic gums, frankincense and myrrh that are gathered from trees. The oldest users of perfumes in a culture were the Egyptians, then the ancient Chinese, Hindus, Israelites, Carthaginians, Arabs, Greeks and Romans. The earliest use of perfume bottles dates back to 1000 BC in Egypt. The Egyptians invented glass and perfume bottles were one of the first common uses for glass. Some manuscripts from the reign of the Egyptian pharaoh, Khufu (circa 2700 BC), record the use of fragrant herbs, choice oils, perfumes and temple incense. There is also telling of healing salves made of fragrant resins. The most famous Egyptian fragrance, kyphi (which translates to welcome to the gods) was said to induce hypnotic states. The city of the sun, Heliopolis, burned resins in the morning, myrrh at noon, and kyphi at sunset to the sun god Ra. Kyphi was used for purposes other than religious. It was used to induce sleep, alleviate anxieties, increase dreaming, eliminate sorrow, treat asthma and act as a general antidote to toxins. Recipes are recorded for mixing and preparing cubes of incense made from a mixture of ground gums and plants with honey. The technique was later adapted by the Babylonians, Romans and Greeks. The next stage in the use of perfumes and fragrances came with the adoption of the distillation of essential oils and the use of aromatics in the first century AD. The first written description of a still in the Western world is one invented by a woman, Maria Prophetissima. It is described in an Alexandrian text. Her design was used initially to distill essential oils but also proved useful for alcoholic beverages. Distillation of essential oils and the use of aromatics migrated to the Far East. Numerous texts related to aromatherapy were published in China as early as 1100 AD. The 16th C. Chinese text, Materia Meica Pen Tsao, discusses 2,000 herbs and 20 essential oils with their supposed effects on various health conditions. Jumping to Western Europe and America, the 19th century saw some important changes in the world of fragrance. The first synthetic fragrance, coumarin, was produced in 1868 in France. It smells like new-mown hay. Twenty years later, musk, vanilla and violet were synthesized. France also became the leader in re-establishing the therapeutic uses of fragrance. The term, aromatherapy was coined in 1928 by the French chemist, Rene-Maurice Gattefoss. His interest in using essential oils therapeutically was initiated by a laboratory explosion in his familys perfumery business. His hand was severely burned and he plunged the injured hand into a container of lavender oil. The hand healed quickly. His work in this new field of therapy was the basis for other investigators including the French doctor Jean Valnet and the Austrian biochemist, Marguerite Maury. During World War II, Dr. Valnet used essential oils such as thyme, clove, lemon and chamomile on wounds and burns. He also later found success in treating some psychiatric problems with fragrances (aromatherapy). More on modern perfume manufacture A good outline on the modern manufacture of perfume follows: Diverse manufacturing processes supply the perfumer with the hundreds of ingredients that could potentially enter into a composition. From the first distillation techniques to chemical synthesis, each process is adapted to a type of raw material in the search for its essence. EXPRESSION Only citrus fruits have peels that are rich enough in natural essences to make the expression process worthwhile. After the peel has been removed from the fruit, it is pierced with numerous small holes and pressed mechanically. The resulting liquid is allowed to settle and then filtered through wet paper. This separates the aqueous parts from theessential oils. The cold-press process is particularly well suited to oranges, lemons and other citrus fruits whose bright and fresh odor would not survive a treatment involving heat. DISTILLATION Distillation relies on evaporation to separate the solids from the various, volatile elements present in a blend. A mixture of water and odoriferous plant material is heated. The steam, carrying with it the odoriferous elements of the blend, escapes into the distillation column, where it is chilled and then collected in a florentine flask. After a period of decantation, the water separates from the odoriferous elements which are collected and named "essences". EXTRACTION When a solvent enters in contact with plant material, it absorbs all the odoriferous substances contained in that material. Traditionally this method - called ENFLEURAGE - involved the use of coldfat. The result of this operation was a pomade or an odoriferous oil. Today fat is replaced by volatile solvents (ethanol, methanol, hexane, toluene, butane or carbon dioxide) which are heated. These solvents are then eliminated through evaporation. What is left is a waxy substance called the concrete. Alcohol is added to the concrete and the mixture is heated and then chilled. During this process, plant matter and waxes are removed from the concrete. Once the alcohol is removed through evaporation, all that is left is the absolute. ENFLEURAGE Enfleurage - the oldest extraction process - involved the use of cold fat . Today it has been almost completely abandoned. It was used to extract the oils from fragile flowers such as orange blossoms, jasmine or tuberose. The hand-picked petals were deposited in a single layer on a pane of glass called "chassis", that was covered with a film of animal fat. After 24 or 48 hours ( 72 hours for the tuberose), the spent petals were carefully removed. This process was repeated several times, until the fat was saturated with floral oils. Once the enfleurage process was completed, the fatty pomade - saturated with odors - was scraped off and washed with wine spirits. The resulting substance was an infusion. SOFTACT When put under pressure at a temperature below 40o C, CO2 enters a supercritical, fluid state. It assumes the properties of a solvent but has the fluidity of a gaseous substance. Thanks to the SOFTACT, it is now possible to obtain extracts whose quality and purity are without par. Indeed, these extracts do not contain any solvents and have not been processed at the usual high temperatures. This is truly a, SOFT EXTRACTION method. CO2 makes it possible to extract odoriferous substances of low volatility, such as those contained in spices, for example. CO2 produces excellent results with dry raw materials that don't do well with the traditional extraction techniques. CO2 used is recycled during the process. SYNTHETIC MOLECULES Once anewmolecule has been selected - following one or several years of intensive research - the most sophisticated techniques are applied in an effort to manufacture it on a large scale, while ensuring its purity and stability. The whole manufacturing process for each of these new molecules can vary in length and complexity, but each of them is the object of an extensive, in-depth study. For instance, to obtain POLYWOOD from pure geraniol, the following steps are necessary : chlorination, distillation, cyclisation, hydrogenation and other esterifications ... a total of six months of various processes that will finally yield the raw material in a state that is usable. The complexity of each chemical reaction as well as the number of successive steps required definitely affect the cost of a raw material and the time needed for manufacturing it. Therefore it is essential to optimize the whole chain of production. (source:  HYPERLINK "http://www.osmoz.com/static/manufacturing-techniques" http://www.osmoz.com/static/manufacturing-techniques) More on the sense of smell Our sense of smell comes about because of specialized nerves in our nose in a specialized area known as the olfactory bulb. Supposedly we are able to distinguish over 10,000 different odor molecules. Inhaled air through the nose passes over a bony plate that contains millions of olfactory receptor neurons in an epithelial cover. These olfactory nerves have cilia extending out into a mucosal lining that is exposed to the atmosphere. The cilia contain olfactory receptors which are specialized proteins that bind low molecular weight molecules (odorants). Each receptor has a pocket (binding site) that has a particular shape that will match either a specific molecule or a group of structurally similar molecules. Research done by Linda Buck and Richard Axel (joint recipients of the 2004 Nobel Prize in Physiology) suggests some 1,000 genes that encode the olfactory receptors for a particular type of odorant molecule. The interaction of the right molecule with the right receptor causes the receptor to change its shape (called its structural conformation). The conformational change generates an electrical signal that goes to the olfactory bulb and then to the areas of the brain where any one nerve impulse is interpreted as a particular smell. Within the olfactory bulb it is thought that groups of olfactory receptors produce spatial patterns of olfactory bulb activity that are characteristic for a given odorant molecule or a blend of odorant molecules. These spatial patterns of activity create the information that leads to the recognition of odor quality and intensity between odors. The information is processed at higher levels of the olfactory system and in the brain to produce the perception of smell.  INCLUDEPICTURE "http://www.nobelprize.org/nobel_prizes/medicine/laureates/2004/illpres/images/olfactory.jpg" \* MERGEFORMATINET  (source:  HYPERLINK "http://www.nobelprize.org/nobel_prizes/medicine/laureates/2004/illpres/images/olfactory.jpg" http://www.nobelprize.org/nobel_prizes/medicine/laureates/2004/illpres/images/olfactory.jpg) Buck and Axel studied a type of cell found in the nose called olfactory receptor cells, and a family of proteins called receptor proteins found in those cells. By studying mouse olfactory receptor cells, they found that each such cell contained only one type of receptor protein. In mice there are over 1,000 different kinds of receptor proteins, although humans may possess only about 350. A relatively large part of the genome of any given mammal is devoted to coding for receptor proteins. With so many different kinds of receptor proteins, as much as 3% of a mammals gene codes for the proteins involved in odor reception. Proteins are long chain-like molecules, made by joining together many amino acid molecules. Receptor proteins are found at the surfaces of receptor cells, and the proteins snake in and out of the cell membrane, crossing it seven times. In the process, receptor proteins are twisted and bent into different shapes, creating cavities of different shapes and sizes. Each receptor protein has a different cavity shape. Odorant molecules can dock with these cavities in the receptor proteins. The shape of the cavity of a particular receptor protein is shaped to allow only members of specific families of molecules to dock with it, in the familiar lock-and-key manner of protein-substrate chemistry. This means that each kind of receptor protein responds to only a specific family of compounds. While a human may only have 350 or so different kinds of receptor cells, many odors are made of combinations of substances. Humans can discern as many as 10,000 different odors, that is, 10,000 different combinations of substances. In addition, within a chemical family, different members may not bind to the same receptor protein, allowing additional levels of nuance in the smell that is perceived. More on specialized smell in fish Although perfume is of great interest to humans, the function of smell in non-humans is more than just imbibing on pleasant odors. Being able to smell particular chemicals serves a most interesting purpose for salmontheir ability to return to their place of birth several years later to repeat the reproductive cycle. Classic experiments done by Arthur D. Hasler in the 1950s clearly demonstrated that salmon can smell particular chemicals in a stream that are associated with the migration route that the salmon takes after hatching to return to the ocean. If their nostrils are blocked, the salmon are unable to follow a particular stream of water that contains the chemical clues. The memory of those smells serves the salmon several years later when they begin the migration from the sea back to the fresh water stream, a distance of 800 to 900 miles away where they developed in and hatched from eggs. It is not known just exactly how the mature salmon find their way along the coastline (Pacific and Atlantic) to zero in on a particular fresh water river that empties into the ocean. There are ideas that for the ocean portion of the return trip, salmon use some navigational tools in the open water that include day length, the suns position and the polarization of the light that results from the angle in the sky, the earths magnetic field, water salinity and temperature gradients. Whatever the combination of tools, the salmon are able to find where their natal waters discharge into the ocean. Young salmon (smolts) are particularly sensitive to the unique chemical odors of their locale when they begin their downstream migration to the sea. Odors that the smolts experience during this time of heightened sensitivity are stored in the brain and become important direction-finding cues years later, when adults attempt to return to their home streams. In one early experiment, salmon that were reared in one stream and then moved to a hatchery during the smolt stage returned to the hatchery, demonstrating the crucial role of imprinting during the transformative period of the fishs life. Recent work has suggested young salmon may go through several periods of imprinting, including during hatching and while emerging from their gravel nest. (A good reference on studying olfaction in salmon in detail is found at  HYPERLINK "http://fish.washington.edu/research/publications/ms_phd/Havey_M_MS_Sp08.pdf" http://fish.washington.edu/research/publications/ms_phd/Havey_M_MS_Sp08.pdf. More on specialized smell in dogs When it comes to detecting odors, dogs have a very highly developed sense of smell, in part because a larger portion of their brain is designed for neural activity from their nasal passages. A comparison of humans with different dog breeds and their neural capacity is shown below: Table: Scent-Detecting Cells in People and Dog Breeds Number of Scent ReceptorsSpecies(millions)Humans5Dachshund125Fox Terrier147Beagle225German Shepherd225Bloodhound300 Inside the nose, receptor cells are attached to a tissue called the olfactory epithelium. In humans, the olfactory epithelium is rather small, and only covers a small part of the surface of the inside of the nasal cavity near the cavitys roof. In dogs, however, the olfactory epithelium covers nearly the entire surface of the interior of the nasal cavity. On top of this, a long-snouted tracking dog like a bloodhound or a basset hound may have a considerably larger nasal cavity than a human. All in all, the olfactory epithelium of a dog may have up to fifty times the surface area as that of a human. While a human may have around 3 cm2 of olfactory epithelium, a dog might have up to 150 cm2. A dogs wet nose also helps it smell more acutely, as odorants are captured as they dissolve in the moisture. The shape of the interior of a dogs nasal cavity also allows odors to be trapped inside during inhalation, without being expelled during exhalation. This allows odorants to concentrate inside the dogs nose for easier detection. When dogs exhale, the spent air exits through the slits in the sides of their noses. The manner in which exhaled air swirls out actually helps usher new odors into the dogs nose. This also allows the dog to sniff more or less continuously. And they smell stereophonically, that is, they can determine the direction of the odorant molecules depending on which nostril detects the odor. A dramatic result of all of these adaptations is that dogs can smell certain substances at concentration up to 100 million (1 x 108) times lower than humans can. A 3-D model of a dogs nasal passage and neural connections:  The canine nasal airway: (a) Three-dimensional model of the left canine nasal airway, reconstructed from high-resolution MRI scans. (b) The olfactory recess is located in the rear of the nasal cavity and contains scroll-like ethmoturbinates, which are lined with olfactory epithelium. The olfactory (yellowish-brown) and respiratory (pink) regions shown here correspond to the approximate locations of sensory (olfactory) and non-sensory (squamous, transitional and respiratory) epithelium, respectively ( HYPERLINK "http://rsif.royalsocietypublishing.org/content/early/2009/12/09/rsif.2009.0490.full" \l "ref-4" Cravenet al. 2007). (Source:  HYPERLINK "http://rsif.royalsocietypublishing.org/content/early/2009/12/09/rsif.2009.0490/F1.expansion.html" http://rsif.royalsocietypublishing.org/content/early/2009/12/09/rsif.2009.0490/F1.expansion.html) Beagles, bloodhounds, and basset hounds have been bred to have especially keen senses of smell, even for dogs. They can be trained to discriminate between various chemical odors, sniffing out land mines, bombs in luggage, drugs at border crossingsand now they are being used in medical diagnosis (cancer in particular). Dogs are trained to detect odors, too faint for humans to smell, that indicate a diabetic patient might be about to go into insulin shock, a condition that results when blood sugar levels drop dangerously low, and can lead to coma and even death. When the dog smells insulin shock on the way, it can alert the patient to take preventive measures, like eating something sweet. If the insulin shock comes while the patient is asleep, a barking dog can be a lifesaver. In the future, dogs may also be used to smell cancers while still too small to be detected by conventional means. Some studies that have been done with what are called sniffer dogs are able to detect some of the chemicals being exhaled by patients with lung cancer. In some well controlled experiments, dogs were able to detect lung cancer from exhaled breath in 71 of 100 test cases and determined that 372 of 400 other patients did not have lung cancer. In addition, the dogs could discern lung cancer from other lung problems such as chronic obstructive pulmonary disease, even sniffing accurately through the exhaled breath of patients that just smoked a cigarette. The dogs are detecting volatile organic compounds being emitted from cancerous cells in the very early stages, which other medical tests or diagnostic technologies are not able to do. Other studies have shown that urine of cancer patients contain volatiles that are detectable by dogs. The ideal would be to identify the particular marker molecules after which an electronic detector might be developed. More on Pheromones Chemical senses are the oldest of senses, shared by all organisms including bacteria. Very recently, it has been determined that several species of bacteria can detect very specific chemicals. Several species of soil bacteria have their own noses for detecting airborne ammonia, an important nitrogen source for the bacterias protein metabolism. Most animal olfactory systems have a large range of relatively non-specific olfactory receptors, which means that almost any chemical in the rich chemical world of animals will stimulate some olfactory sensory neurons and can potentially evolve into a pheromone. A pheromone is a molecule used for communication between animals of the same species. [The word pheromone comes from the Greek, pherein, to carry or transfer and hormon, to excite or stimulate.] Across the animal kingdom, more interactions are mediated by pheromones than by any other kind of signal. There is a certain commonality between vertebrates and invertebrates in terms of the pheromones produced and in the range of behaviors that pheromones influence. Insects such as ants use pheromones to direct their colleagues to a food source and find their way back to the colony. They also have other pheromones to mark the way to new nest sites during emigration aggregate mark territories recognize nest mates. Mating activities of moths depend upon the male detecting the odors emitted by the female of the species. Chemical knowledge of this mating pheromone or sex attractant has been used to lure male moths into traps to limit reproduction of moths that are destructive to plants, such as the Gypsy moth. But other animals as large as the elephant make use of pheromones, primarily for reproductive purposes (sexual signaling). An interesting note is the fact that the pheromone used by elephants is the same molecule used by 140 species of moth! Yet there is no interaction between the two groups of animals because the receptors and the signals produced are different! Dogs like many other mammals (except humans) respond to pheromones meant to indicate mating readiness and other sexual details. Since we were talking previously about the highly sensitive olfactory system in the dog, it turns out that the dog possesses a special olfactory structure in its nose for detecting pheromones in the mix of chemicals that come through its nasal channels. This structure is called Jacobsons organ; it is located in the bottom of the dogs nasal passage. The pheromone molecules that the organ detectsand their analysis by the braindo not get mixed up with odor molecules or their analysis, because the organ has its own nerves leading to a part of the brain devoted entirely to interpreting its signals. It's as if Jacobson's organ had its own dedicated computer server. (from  HYPERLINK "http://www.pbs.org/wgbh/nova/nature/dogs-sense-of-smell.html" http://www.pbs.org/wgbh/nova/nature/dogs-sense-of-smell.html) Some known pheromone molecular structures are shown here.  SHAPE \* MERGEFORMAT  Chemical composition of certain pheromones: (1) sex attractant of female of Asiatic silkworm, (2) marking substance of certain bumblebees, (3) aphrodisiac of male of Danaidae butterfly, (4) attractant of female of gypsy moth, (5) component of marking secretion of a rodent (clawed jird), (6a, 6b, 6c) three components of clustering pheromone of Scolytus bark beetle, (7) anxiety pheromone of Lasius ant (source is  HYPERLINK "http://encyclopedia2.thefreedictionary.com/Pheromone" http://encyclopedia2.thefreedictionary.com/Pheromone) There are defined criteria for a pheromone. The general size of pheromone molecules is limited to about 5 to 20 carbons and a molecular weight between 80 and 300. This is because below 5 carbons and a molecular weight of 80, very few kinds of molecules can be manufactured and stored by glandular tissue. Above 5 carbons and a molecular weight of 80, the molecular diversity increases rapidly and so does the olfactory efficiency. Once you get above 20 carbons and a molecular weight of 300, the diversity becomes so great and the molecules are so big that they no longer are advantageous. They are also more expensive to make and transport and are less volatile. In general, most sex pheromones are larger than other pheromones. In insects, they have a molecular weight between 200 and 300 and most alarm substances are between 100 and 200. ( HYPERLINK "http://www.angelfire.com/ny5/pheromones5/bibliography.html" \l "wilson" Sociobiology: The Abridged Edition, 1980, 114) (from  HYPERLINK "http://www.angelfire.com/ny5/pheromones5/structure.html" http://www.angelfire.com/ny5/pheromones5/structure.html) Besides the category of pheromone associated with sexual signaling, there are alarm pheromones that are released to promote fight and flight reactions in receivers. Many ant species release the same pheromones to repel an opponent and an alarm to recruit fellow ants for assistance in a battle with the invaders. In other animals, alarm pheromones are used to make flesh unpalatable or toxic to a predator. These substances would be released by an injured animal. There are a variety of sea organisms that use this technique. More on olfactory fatigue One of the interesting neural responses of our olfactory system is a disappearance of the recognition or registration of a particular smell in the air being inhaled, over a short period of time. The neural receptors for smell eventually stop sending signals to our brain for interpretation of a particular smell. This is known as olfactory fatigue. Have you ever noticed a particular scent upon entering a room, and then not noticed it ten minutes later? This is due to olfactory fatigue. The olfactory sense is unique because it relies on mass, not energy to trigger action potentials. Your ears do not "stop" hearing a sound after a certain period of time, nor do your eyes stop seeing something you may be staring at. This is because both the ears and the eyes rely on energy to trigger them, not mass. In the nose, once a molecule has triggered a response, it must be disposed of and this takes time. If a molecule comes along too quickly, there is no place for it on the olfactory hairs, so it cannot be perceived. To avoid olfactory fatigue, rabbits have flaps of skin that open and close within the nostrils. This allows for short, quick sniffs and lets the rabbit "keep in close odor contact with its environment." When we wish to fully perceive a scent, we humans also smell in quick, short sniffs, often moving the source of the smell in front of one nostril then the other. This behavior also prevents odor fatigue. HYPERLINK "http://www.macalester.edu/academics/psychology/whathap/ubnrp/smell/references.html" (Stoddard & Whitfield, 1984) (source:  HYPERLINK "http://www.macalester.edu/academics/psychology/whathap/ubnrp/smell/odor.html" http://www.macalester.edu/academics/psychology/whathap/ubnrp/smell/odor.html) An interesting trick or technique to counter olfactory fatigue in perfume shoppers is to have containers of coffee beans on the store counter which tend to resetolfaction.Anosmiais the permanent loss of the sense of smell, and is different from olfactory fatigue. (Wikipedia,  HYPERLINK "http://en.wikipedia.org/wiki/Olfactory_fatigue" http://en.wikipedia.org/wiki/Olfactory_fatigue) Connections to Chemistry Concepts (for correlation to course curriculum) Organic compoundAny chemical that contains carbon (except carbon monoxide, carbon dioxide and metal carbonates) is considered an organic compound. Because of the bonding based on the carbon atom, organic compounds have an almost infinite number of configurations with important functional groups attached. This is particularly useful for the perfume-making business as any one perfume can contain from 60 to 300 different molecules, many of them organic. The size of the molecules of organic compounds is wide-ranging. It is thought that a truck tire of synthetic or natural rubber, an organic polymer, is a single molecule! PheromoneThis category of biological molecule, particularly important in communication between various groups of insects, is organic and has a general size of between 5 and 20 carbons with a molecular weight between 80 and 300. The size and weight limits are related to molecular diversity and olfactory efficiency. Going above these limits reduces the effectiveness of the pheromones which are dependent on gaseous dispersal, hence volatility. Kinetic molecular theory of gasesBecause gas molecules are constantly in motion, volatile substances in perfumes can reach our nose from a source at some distance from us. Phase changealthough a perfume is applied as a liquid, it can only be detected in our noses if the perfume undergoes a phase change from liquid to a gas (evaporation). But to be detected in our nose, the gas has to then go into solution. Possible Student Misconceptions (to aid teacher in addressing misconceptions) Human pheromones have been identified since they are sold for enhancing sexual attraction. Although pheromones are purportedly available as human pheromones and sold for enhancing sexual attraction in various perfumes, there is no evidence that human pheromones exist or that they enhance sexual attraction as in other animals. Some studies have tried to link the odor of mens sweaty underwear to womens sexual responses, but most scientific studies do not find a link. Underarm sweat from male or female when placed on the upper lip of females is found to affect the timing of the menstrual cycle. There are plenty of odors that females respond to, but they are not specific responses as in the case of other animals and insects related to pheromone emissions. A specific human pheromone has yet to be isolated chemically. We have a limited number of odors that we can detect. OK, this isnt really a misconception, but it comes close; it seems as if our olfactory system can detect up to 10,000 different odorants, which seems to be quite extensive! If we synthesize a molecule that occurs in nature, then it is natural. For a chemical to be natural, it has to come from some source in nature, either living (plant or animal) or non-living such as that which is extracted from the earth. But to put together (synthesize) a molecule that has the exact same structure as that extracted from natural sources still is synthetic. Anticipating Student Questions (answers to questions students might ask in class) Is smell different than taste? Do fish smell food or do they taste the food? Depending on the type of animal, there can be separate locations for taste detection (e.g., taste buds or receptors) and smell detection (e.g., olfactory receptors). In the case of fish, there are olfactory receptors in their nasal passages for the detection of various types of molecules in the water including those that identify the type of water (related to homing instincts in salmon) or the presence of another fish of the same species. So fish smell food. But they are also able to taste with taste buds on their lips and on the roof of their mouths as well as on the gills. They do not have taste buds on their tongue as is true for humans. For humans, we taste as well as smell food with olfactory receptors in the nose and taste buds on the tongue as well as in the back of the throat. The taste buds detect certain tastessalt, sweet, bitter, sour and umami (deliciousness). Smell comes in multiple categories creating a complex of odors. Sometimes smell and taste work in conjunction with each other to produce a particular taste. If your taste buds are blocked, as in a cold, some flavors of food are not detected. Chocolates flavor depends on smell as much as taste. If you block out smell, the only components of the chocolate flavor will be sweetness and bitterness (from the taste buds). What is a musk-based perfume? Can it be synthetic? A musk-based perfume is one that has the odor of a variety of animals that produce secretions from special glands, musk glands. The perfume can be either synthetic, or its scent can be derived from the musk glands of certain animals, in particular the Asian musk deer. In this day and age, most musk-based perfumes are synthetic, not natural. The primary ingredient is the organic molecule, muscone, a 15-carbon ring with several groups attached. Its molecular weight makes it a less volatile substance than is normally required for perfumes. But that characteristic makes it useful for containing (trapping) other more volatile perfume molecules, acting as a reservoir to provide a constant source of odor that is released over time. To be smelly, a perfume molecule has to be able to easily evaporate but not all at once! The muscone molecule can be produced through animals, in this case, civet cats that are fed coffee beans. The beans are not digested but are excreted intact, coated with their muscone-like substance called civetone. The beans are then washed of their civetone and the collected wash is used in the preparation of musk-based perfume. A synthetic musk odor is often produced with the molecule called galaxolide, rather than trying to synthesize muscone. It is used in perfumes as well as in soaps, cosmetics and detergents. Why does the initial odor of a perfume disappear even though the person is still in the room? The molecules responsible for the odor of the perfume are still in the air and reaching a persons nose. But the persons nervous system has reached what is known as olfactory fatigue. If the person who no longer smells the odor were to leave the area of the perfume, then return, that person would again smell the perfume for another period of time before sensory fatigue sets in. How do the molecules of an odor become a sensation of smell? When the molecules associated with an odor reach the nerve endings of the olfactory sensors in the nose, they must first go into solution (the mucosa).  INCLUDEPICTURE "http://www.senseofsmell.org/sosi10/imagesu/lesson1-leffingwell.jpg" \* MERGEFORMATINET  (source:  HYPERLINK "http://www.senseofsmell.org/smell101-detail.php?id=1&lesson=How%20Does%20the%20Sense%20of%20Smell%20Work" http://www.senseofsmell.org/smell101-detail.php?id=1&lesson=How%20Does%20the%20Sense%20of%20Smell%20Work) This solution bathes cilia that are part of nerve endings (olfactory nerve endings) which are an extension of what is known as the olfactory bulb. Within the olfactory bulb are nerve endings that connect to the cilia-olfactory nerve endings, carrying a nerve impulse to the brain. The stimulation of the nerve endings is accomplished through specialized proteins in the cilia that bind low molecular weight molecules (odorants). The binding of the odorant molecules to the specialized proteins causes a change in the structure of the specialized proteins which in turn sets off an electrical signal that passes into the olfactory bulb and on to the brain for interpretation as a particular smell. Why are dogs more sensitive to smell than humans? If you look at the sensory area for smell in a dogs brain, it is apparent that it is much more extensive than in our brains. It is estimated that a dog has some 20 to 40 times as many receptors as humans. If you test a dogs ability to smell the particularly odoriferous molecule hydrogen sulfide, it is found that the lowest concentration of hydrogen sulfide in air that is detected is 10-13 % (0.00000000000001%, or 1000 ppt). The lowest concentration of hydrogen sulfide detected by humans is 10-6 % (0.0000001% or 100 ppm). Note that the MSDS for hydrogen sulfide lists the short term exposure limit (10 minutes) at 15 ppm, which means we cant even detect it at its toxic levelbut dogs can. In-class Activities (lesson ideas, including labs & demonstrations) Students could experiment with olfactory fatigue. Here are several Web sites that outline such experiments for investigating olfactory fatigue including  HYPERLINK "http://www.sciencebuddies.org/science-fair-projects/project_ideas/HumBio_p031.shtml" \l "materials" http://www.sciencebuddies.org/science-fair-projects/project_ideas/HumBio_p031.shtml#materials and  HYPERLINK "http://academic.evergreen.edu/curricular/perception/Lab1006.htm" http://academic.evergreen.edu/curricular/perception/Lab1006.htm. This latter Web site has very good questions for the students to think about with regard to the topic of olfactory fatigue. Another Web site on olfactory fatigue activity is found at  HYPERLINK "http://faculty.washington.edu/chudler/chems.html" http://faculty.washington.edu/chudler/chems.html.). The teacher guide for this activity is found at  HYPERLINK "http://faculty.washington.edu/chudler/pdf/chemstg.pdf" http://faculty.washington.edu/chudler/pdf/chemstg.pdf. Students could synthesize esters which are normally used as flavoring in foods, but for this exercise would simply be the production of pleasantly smelling compounds that they can recognize. Ester synthesis involves the use of concentrated sulfuric acid. But if done in small quantities it presents less of a lab safety issue. Or the teacher can add the acid for the students at the correct step in the procedure. Refer to the following Web site for a complete lab exercise in ester synthesis:  HYPERLINK "http://www.nuffieldfoundation.org/practical-chemistry/making-esters-alcohols-and-acids" http://www.nuffieldfoundation.org/practical-chemistry/making-esters-alcohols-and-acids. Although this ChemMatters article deals with smell, students could map their tongue for the locations of the principle tastes of salt, bitter, sweet and sour (acidic). Smell is often involved with a particular taste. This exercise would also point out to students the specificity of neural receptors. Most biology lab manuals contain the exercise procedure. A printable outline of the tongue with the locations on the tongue for the different categories of taste is found at  HYPERLINK "http://www.teachervision.fen.com/tv/printables/orange/SL-27.pdf" http://www.teachervision.fen.com/tv/printables/orange/SL-27.pdf. A Web site for the lab procedure can be found at  HYPERLINK "http://faculty.washington.edu/chudler/chtaste.html" http://faculty.washington.edu/chudler/chtaste.html. You can also actually see the taste buds on the tongue and compare the number for different people. See the following Web site for the simple instructions:  HYPERLINK "http://www.bbc.co.uk/science/humanbody/body/articles/senses/tongue_experiment.shtml" http://www.bbc.co.uk/science/humanbody/body/articles/senses/tongue_experiment.shtml. Additional background information and discussion about the integral role of smell with taste and touch for the sensations of what some people would call the flavor of foods is found at  HYPERLINK "http://www.tastescience.com/default.html" http://www.tastescience.com/default.html. Out-of-class Activities and Projects (student research, class projects) Instructions for making your own perfume can be found at  HYPERLINK "http://www.aromasuccess.com/howtomakeperfume.html" http://www.aromasuccess.com/howtomakeperfume.html and  HYPERLINK "http://tipnut.com/how-to-make-perfume/" http://tipnut.com/how-to-make-perfume/. A very detailed reference on making your own perfumes is found at  HYPERLINK "http://www.aromasuccess.com/howtomakeperfume.html" http://www.aromasuccess.com/howtomakeperfume.html. Commercial kits for making both perfumes and cosmetics can be purchased at Edmund Scientifics Web address:  HYPERLINK "http://www.scientificsonline.com/science-kits/diy-kits.html" http://www.scientificsonline.com/science-kits/diy-kits.html. Students could study the history of the development of and, in the 19th century to the present, the manufacture of perfumes and aromatic oils. A starting Web site would be  HYPERLINK "http://inventors.about.com/gi/dynamic/offsite.htm?site=http://www.parfumsraffy.com/history.html" http://inventors.about.com/gi/dynamic/offsite.htm?site=http://www.parfumsraffy.com/history.html. Another Web site that discusses the one hundred most important perfumes and fragrances is  HYPERLINK "http://boisdejasmin.com/2011/02/fragrances-that-influenced-perfume-history-100-great-fragrances-series-110.html" http://boisdejasmin.com/2011/02/fragrances-that-influenced-perfume-history-100-great-fragrances-series-110.html. Students could research the chemistry behind the modern manufacturing of perfumes. A starting reference that utilizes an interview with a perfumer would be  HYPERLINK "http://www.osmoz.com/static/manufacturing-techniques" http://www.osmoz.com/static/manufacturing-techniques. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  Luebe, M. Perfume. ChemMatters 1992, 10 (1), pp 811. This is a very complete description of the various techniques involved in current manufacture of perfumes. There is an introductory history of perfumes as well. Arrigo, J. The Mystique of Musk. ChemMatters 1991, 9 (2), pp 1215. This article provides a very detailed history of the chemists who developed synthetic perfumes including musk. Molecular structures are also included. Kimball, A. Human Pheromones: The Nose Knows. ChemMatters 1997, 15 (2), pp 810. Although the title suggests the article is only about pheromones, the general content is about aromas in general. The suggestion about human pheromones is not supported by scientific evidence to date. Fruen, L.. Cleopatras Perfume Factory and Day Spa. ChemMatters 2004, 22 (3), pp 1315. This article is a good read concerning the ancient art of making perfumes, ointments and cosmetics by Cleopatra. Included in the article is some of the chemistry behind these products. Kimbrough, D. How We Smell and Why We Stink. ChemMatters 2011, 19 (4), pp 810. This article may appeal to students because it explores those bodily odors that do not qualify as attractive perfumes. It explores the origins of the typical teenager odors! Vos, S. Sniffing Landmines. ChemMatters 2008. 28 (2), pp 79. This article discusses how dogs are able to detect landmines through training to recognize specific volatile chemicals that emanate from the buried explosive device. Web sites for Additional Information (Web-based information sources) More sites on the workings of a gas chromatograph (GC) and mass spectrophotometer A video on GC can be found at  HYPERLINK "http://www.youtube.com/watch?v=q0pM-k0SvOQ" http://www.youtube.com/watch?v=q0pM-k0SvOQ, and a video on GC and mass spec can be viewed at  HYPERLINK "http://www.youtube.com/watch?v=08YWhLTjlfo" http://www.youtube.com/watch?v=08YWhLTjlfo. A complete and clear explanation of a GC and mass spec can be found at  HYPERLINK "http://www.youtube.com/watch?v=dffeiLgeKx8&feature=related" http://www.youtube.com/watch?v=dffeiLgeKx8&feature=related. More sites on perfume manufacture A number of Web sites that detail the history of humankind concocting perfumes and fragrances include:  HYPERLINK "http://www.healthy.net/asp/templates/article.asp?PageType=article&ID=1712" http://www.healthy.net/asp/templates/article.asp?PageType=article&ID=1712,  HYPERLINK "http://inventors.about.com/gi/dynamic/offsite.htm?site=http://www.healthy.net/asp/templates/article.asp%3FPageType=article%26ID=1712" http://inventors.about.com/gi/dynamic/offsite.htm?site=http://www.healthy.net/asp/templates/article.asp%3FPageType=article%26ID=1712, and  HYPERLINK "http://www.aromasuccess.com/howtomakeperfume.html" http://www.aromasuccess.com/howtomakeperfume.html. Additional Web sites that detail the various steps in the manufacture of perfumes are found at  HYPERLINK "http://www.madehow.com/Volume-2/Perfume.html" http://www.madehow.com/Volume-2/Perfume.html and  HYPERLINK "http://www.aromasuccess.com/howtomakeperfume.html" http://www.aromasuccess.com/howtomakeperfume.html. HYPERLINK "http://www.madehow.com/Volume-2/Perfume.html and "   More sites on a dogs sense of smell This is a Nova Web site about dogs and their use in tracking things:  HYPERLINK "http://www.pbs.org/wgbh/nova/nature/dogs-sense-of-smell.html" http://www.pbs.org/wgbh/nova/nature/dogs-sense-of-smell.html. It includes an extensive set of references that are Web-accessible. An academic article from a science journal about investigating the ability of canines to detect cancer is found at  HYPERLINK "http://erj.ersjournals.com/content/early/2011/08/05/09031936.00051711" http://erj.ersjournals.com/content/early/2011/08/05/09031936.00051711. A series of references on dogs about their sense of smell plus training is found at the Web site for Scientific American Frontiers,  HYPERLINK "http://portal.acs.org/portal/PublicWebSite/education/resources/highschool/chemmatters/archive/WPCP_008628" http://portal.acs.org/portal/PublicWebSite/education/resources/highschool/chemmatters/archive/WPCP_008628. More sites on pheromones Current thinking on human pheromones and the role of odors in human interaction can be found at  HYPERLINK "http://www.scientificamerican.com/article.cfm?id=pheromones-sex-lives" http://www.scientificamerican.com/article.cfm?id=pheromones-sex-lives. Another site that provides a very extensive background on pheromones and their use by various groups of animals is found at  HYPERLINK "http://catdir.loc.gov/catdir/samples/cam033/2002024628.pdf" http://catdir.loc.gov/catdir/samples/cam033/2002024628.pdf. An example of how scientists go about studying and deciphering ant behavior that includes using pheromones is found at  HYPERLINK "http://beheco.oxfordjournals.org/content/18/2/444.full" http://beheco.oxfordjournals.org/content/18/2/444.full. A complementary article on studying the behavior of ants, in terms of detecting scents, is found at  HYPERLINK "http://blogs.scientificamerican.com/thoughtful-animal/2011/06/23/nosejobs_for_ants/" http://blogs.scientificamerican.com/thoughtful-animal/2011/06/23/nosejobs_for_ants/. A Web site that is all about ants and how they communicate (includes video and drawings of body parts important to the communication) is found at  HYPERLINK "http://blog.wildaboutants.com/2010/06/27/questions-about-ant-pheromones/" http://blog.wildaboutants.com/2010/06/27/questions-about-ant-pheromones/. A college Web site about pheromones might prove useful to students who adopt the topic of pheromones for a research project. The Web site is quite extensive and also readable. The Web address is  HYPERLINK "http://www.macalester.edu/psychology/whathap/UBNRP/pheromone10/pheromones%20intro.html" http://www.macalester.edu/psychology/whathap/UBNRP/pheromone10/pheromones%20intro.html . An extensive collection of Web sites from Scientific American dealing with pheromones can be found at  HYPERLINK "http://www.scientificamerican.com/search/?q=pheromones&x=0&y=0" http://www.scientificamerican.com/search/?q=pheromones&x=0&y=0. More sites on homing traits of salmon One site that summarizes current thinking on the ability of salmon to return to their freshwater site of birth is found at  HYPERLINK "https://www.novapublishers.com/catalog/product_info.php?products_id=26321&osCsid=168c08748e890891d8335a0f23b338ea" https://www.novapublishers.com/catalog/product_info.php?products_id=26321&osCsid=168c08748e890891d8335a0f23b338ea. Additional sites that deal with salmon homing instincts are found at  HYPERLINK "http://fish.washington.edu/research/publications/ms_phd/Havey_M_MS_Sp08.pdf" http://fish.washington.edu/research/publications/ms_phd/Havey_M_MS_Sp08.pdf (a PhD thesis that discusses the experimental setup for evaluating imprinting on salmon). For a story about studying the geomagnetic abilities of salmon in homing from ocean to freshwater see  HYPERLINK "http://www.pnas.org/content/105/49/19096.full" http://www.pnas.org/content/105/49/19096.full. A site that shows the role of amino acids in water that salmon use for homing can be found here:  HYPERLINK "http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0008633" http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0008633. This site gives a very complete discussion of what is known and not know about how salmon find their way from ocean to their site of birth in an inland freshwater stream:  HYPERLINK "http://jeb.biologists.org/content/199/1/83.full.pdf" http://jeb.biologists.org/content/199/1/83.full.pdf. More sites on using animal sense of smell for medical purposes This site,  HYPERLINK "http://www.monell.org/news/news_releases/lung_cancer_odors" http://www.monell.org/news/news_releases/lung_cancer_odors, is from one of the major research centers on smell, the Monell organization. Their news information describes the research into detecting cancer through odors in urine using both animals and electronic chemical sensing. In this case the subjects are mice. But dogs are also known to be able to detect cancer in human patients, both from sensing volatile organic compounds emitted by a person as well as sniffing a patients urine, depending on the type of cancer. Another Web site dealing with cancer detection by dogs is found at  HYPERLINK "http://www.huffingtonpost.com/organic-authoritycom/dogs-smell-cancer_b_976797.html" http://www.huffingtonpost.com/organic-authoritycom/dogs-smell-cancer_b_976797.html. Mascara: That Lush Look You Love! Background Information (teacher information) More on eyelashes For centuries the eyes have been recognized as an important part of physical beauty, especially for women. Attractive womens eyes are often also associated with favorable social status. And in many cultures long full eye lashes are symbols of beauty. On the other hand, loss of eye lashes is seen as a sign of deficiency in women. And so for centuries women have tried a myriad of methods for making their lashes, longer and fuller. From an anatomy and physiology point of view, however, eye lashes serve several functions. They are intended to keep foreign particles or small insects from entering the eyes and causing damage or irritation. Lashes are attached to eyelids in a curved arc designed to channel water away from the eyes, forming another layer of protection from the environment. Lashes are actually sensitive structures, similar to cats whiskers. They trigger the blink reflex response when an object comes too close to the eyes. Lashes are simply hairs that grow from the edge of the eyelid. They are arranged in two or three rows. Each eye has between 100 and 150 individual hairs with upper lids having the greater number. Eyelashes are the widest type of human hair and the most richly pigmented. Each hair is, on average, 8-9 mm long, 7 mm of which extend beyond the eyelid. Lashes grow at a rate of about 0.15 mm per day, which means that if lashes are pulled out they take about eight weeks to fully grow back. Like other human hair, eyelashes are produced from follicles under the skin. Follicles have three stages of growthan actual growing phase, a declination phase and a shedding phase. Each hair is very strongcapable of supporting 100 grams.  Hair growth in humans is different from hair growth in many mammals that shed their hair all at once. Human hair growth is asynchronousthat is, some follicles are experiencing growth while others are in decline or being shed. The eyelash growth cycle is variable, lasting between five and twelve months. The first phase is called anagen. This is the growing phase and lasts about 45 days on average. The normal length of a persons lashes is determined by this phase. In the second stage of growth, called catagen, follicle cells undergo programmed cell death, a process that takes about fifteen days. About 3% of all lashes are in this phase at any given time. The lashes then enter a period of rest, telogen, which can last as long as nine months. Up to 15% of hair is in this phase. At the end of this phase lashes are shed in a process labeled exogen. As this phase ends, anagen begins again. Like all human hair, eyelashes are 85% proteins, primarily keratin and melanin. Water makes up 7 % of human hair and 3 percent is composed of lipids. The keratin is made up of eighteen amino acids, 7 percent water, and low concentrations of trace minerals (e.g., iron, zinc, copper). The following excerpt about keratin is excerpted from the October 2008 ChemMatters Teachers Guide: Keratin is a fibrous protein polymer which [is] a bundle of helical structures held together by four types of bonds. The strongest of these are the polypeptide bonds holding amino acids together. Disulfide bonds (sometimes called cysteine bonds) are cross-linking bonds between adjacent sulfur atoms in keratin. These bonds help hold the helical polymer-like structures together. Hydrogen bonds also hold the peptide strands together, but are weaker than disulfide bonds. Because there are charge centers in the polypeptide strands, there are also ionic bonds (termed salt bonds) that cross-link. The fourth type of bond is a variation of van der Waals forces, but in the context of hair is of minimal importance. The keratin helices can be extremely strong due to the presence of a compound called cysteine disulfide (human hair is about 14% cysteine). When the disulfide bond is present, the sulfur atoms are able to bond with each other in what are known as disulfide bridges. The degree to which they bond determines the stiffness or flexibility of the hair. This is what gives each hair much of its shape. Hair also contains some fats, melanin, trace amounts of metals and about 10% water. The chemical composition of human hair is approximately 45 % carbon, 28 % oxygen, 7% hydrogen, 15 % nitrogen and 5 % sulfur. These elements make up the amino acids, keratin and protein in hair. At least 16 amino acids are present in hair, with cysteine the most important. The lipid component is formed from sterols, fatty acids and ceramides, which are fatty molecules that help hold hair cuticle cells together. More on mascara As the article states, mascara is a cosmetic used to darken, thicken or lengthen eyelashes. Most mascaras are essentially emulsions that are made of the same basic components of pigments, oils, water, waxes, and preservatives. Waterproof mascara, however, contains no water. The components serve several purposespigments for color, waxes or oils to serve as a base, proteins, starches or synthetic polymers as thickeners. PigmentsThe main black pigment in mascara is carbon black, pure carbon that is produced by charring wood or bone. The carbon particles are very small and usually spherical in shape. Carbon spheres fuse together to form chains, and chemical functional groupslike carboxyl or hydroxyl groupsbind to the surface of the carbon particles. Iron oxides, Fe2O3 and Fe3O4, comprise the brown pigment. The article also mentions ultramarine blue pigment which is complex sodium aluminum silicate that also contains sulfur, Na8-10Al6Si6O24S2-4. Compounds derived from coal and tar, once used as pigments, are currently prohibited by law in the United States. The base for most brands of mascara is either an oil like castor oil or mineral oil, or a wax like paraffin, carnauba wax or lanolin. OilsCastor oil is a pale yellow liquid with no odor or taste. Its boiling point is 313 C (595F) and its density is 961kg/m3. It is a triglyceride in which approximately 90 percent of fatty acid chains are ricinoleic acid. Oleic and linoleic acids are the other significant components. Mineral oil is any one of a series of colorless, odorless, light mixtures of alkane hydrocarbons in the C15 to C40 range derived from a mineral source, particularly a distillate of petroleum. It is odorless and tasteless. One drawback is that when it is used in makeup products like mascara it tends to clog pores, a deficiency that can be overcome by purifying and refining it. WaxesLanolin is a yellow waxy substance that is complex and variable in composition. For example, a typical high purity grade of lanolin (97% by mass) is composed predominantly of long chain waxy esters. There are estimated to be between 8,000 and 20,000 lanolin esters in lanolin. Paraffin wax refers to a mixture of alkanes that falls within the C20 to C40 range; they are found in the solid state at room temperature and begin to melt at approximately 37 C. Carnauba wax consists primarily of aliphatic esters (40 wt %), diesters of 4-hydroxycinnamic acid (21 wt %), -hydroxycarboxylic acids (13 wt %), and fatty acid alcohols (12 wt %). The compounds are predominantly derived from acids and alcohols in the C26-C30 range. The wax is obtained from the leaves of the carnauba palm by collecting and drying them, beating them to loosen the wax, then refining and bleaching the wax. There are two main types of mascara currently manufactured. One type is called anhydrous, meaning it contains no water. The second type is made with a lotion base, and it is manufactured by the emulsion method. EmulsionsWater is present in many mascara formulations, and when it is present it is in the form of an emulsion with the oily ingredients. Your students may have studied emulsions, like salad dressings or homogenized milk. Emulsions are a type of colloid in which two liquids that are normally immiscible, are dispersed uniformly throughout each other. Emulsions are typically unstable and will return to their immiscible state unless a stabilizer is added. Examples of emulsions that your students might know are vinaigrettes, milk and mayonnaise. Egg yolk (specifically the lecithin in the yolk) is a common food emulsion stabilizer. Stabilizers in mascara include hydrophyllic compounds like the polymers polyvinylpyrrolidone, acrylates copolymer or methacrylate polymers, or hydrophobic compounds such as carnauba and ozokerite. Emulsion based mascara is made by mixing water and thickeners to make a lotion or cream base. Waxes and emulsifiers are heated and melted separately, and pigments are added. Then the waxes and lotion (aqueous) base are combined in a very high speed mixer or homogenizer that mixes the ingredients at very high speed without incorporating any air. The oils and waxes are broken down into very small beads by the rapid action of the homogenizer and held in suspension in the water. Water Resistant or WaterproofWaterproof mascara is made simply by mixing the non-aqueous ingredients and heating them in a mixing tank. When the mixture reaches a semi-solid state it is pumped into containers and prepared for distribution. One of the ingredients in waterproof mascara that makes it waterproof is dimethicone copolyol, a form of dimethicone. Many of the waterproof mascaras are also silicone based. In addition to the ingredients listed above, some formulas contain alcohol. Stearic acid is a common ingredient of lotion-based formulas, as are stiffeners such as ceresin and gums such as gum tragacanth and methyl cellulose. Some mascaras include fine rayon fibers, which make the product more viscous. Water-resistant mascaras have basis in substances that rebuff water, like dodecane (C12H26). Mascaras designed to lengthen or curl the eyelashes often contain nylon or rayon microfibers. Additionally, ceresin, gum tragacanth, and methyl cellulose are regular ingredients added to act as stiffeners. When you read mascara product labels you read terms like emollients, moisturizers, and thickeners. What are these substances? The waxes and oils listed above are all emollients. Some of the key characteristics required in good emollients are good spreading properties, low toxicity/skin irritation and good oxidative stability. Oleic acid, a major constituent of olive oil, has poor oxidative stability due to the presence of its double bond. Fats and oils are considered saturated if they do not have double bonds. Long-chain alcohols, also called fatty alcohols, are useful as emollients and emulsion stabilizers. Their polar hydroxyl groups orient to the water phase with their fatty chains oriented towards the oil phase. Esters of fatty alcohols and fatty acids make excellent emollients because of their low reactivity and good stability. Skin and hair have their own moisturizing process. As hair cells move toward the surface, lipids, or fatty molecules, are deposited between cells, forming a natural barrier to water loss. If these lipid molecules are disrupted, hair or skin dries out. This is where moisturizers come in. There are three basic types of moisturizers: humectants, emollients and occlusive agents. As described above, emollients help the other substances to spread on the hair or skin. Humectants attract water from below the surface of the hair and help the hair retain it. Common humectants include glycerin, urea, and pyrrolidone carboxylic acid. Occlusive agents form a barrier on the hair and skin to prevent water loss. Waxes, oils, and silicones are frequently used occlusive agents. The waxes described above are also used as thickeners. The article makes special mention of a minor ingredient in some mascarasguanine. Guanine, with a formula of C5H5N5O (see structure at right), is one of the four base pairs in the DNA molecule. It always pairs with cytosine. The substance is derived from purine. Guanine crystals are rhombic platelets composed of multiple transparent layers. They have a high index of refraction that partially reflects and transmits light from layer to layer, thus producing a pearly luster. Guanine used in mascara is usually extracted from the scales of fish, as the article says. It was first isolated from bird droppings which are known as guano, the term also used to refer to bat droppings. Bat excrement also contains guanine and the confusion about the terms guano and guanine led to the misconception that mascara contains bat excrement. For database of mascara ingredients see  HYPERLINK "http://householdproducts.nlm.nih.gov/cgi-bin/household/prodtree?prodcat=Personal+Care&purpose=Eye+Care/Makeup&type=mascara" http://householdproducts.nlm.nih.gov/cgi-bin/household/prodtree?prodcat=Personal+Care&purpose=Eye+Care/Makeup&type=mascara More on cosmetic history Mascara is a cosmetic applied to the eyelashes to make the lashes thicker, longer, and darker. Its use is likely derived from the idea that the eyes are the windows of the soul. Cicero (106-43 BC) is often cited as the earliest definitive statement of the ideaUt imago est animi voltus sic indices oculi.but the belief existed long before him. Egyptian men wore mascara, as did Egyptian women. Ancient drawings of Cleopatra and Nefertiti suggest that mascara and body painting were widely used for protection, celebration, war, and death practices. As the article states, most histories of mascara begin with its use in ancient Egypt as long ago as 3500 BC. The mixture called kohl is one of the earliest forms of mascara. It was probably made of galena (PbS) or lead sulfite, malachite (Cu2CO3 (OH)2), and charcoal or soot blended with water and honey. Bone and ivory were used as applicators. As noted earlier in the Teachers Guide Egyptians used blackened material around the eyes for a very practical reasonto protect the eyes from the hot African sun, similar to the way athletes do so today. Kohl continued to be used as mascara through the Babylonian, Greek and Roman empires, but after the fall of the Roman Empire, kohl fell into disuse on the European continent. Mascara once again became popular during the Victorian era in the 1830s. Attitudes about makeup in general became positive, and as a result Victorian women wore elaborate cosmetics, including mascara, believing that enriching their eyelashes was an essential part of beauty. In many cases women prepared their mascara at home. They tried numerous mascara recipes like elderberries and ashes heated to form a soupy mixture. But it was not until the 1800s that the forerunner of modern mascara was invented. In 1859, oil was first discovered in Pennsylvania, and a chemist named Robert Cheesebrough developed a process for refining the lighter oil fractions and producing a pale colored gel that he called Vaseline, which was patented in 1872.This product is odorless, tasteless and nearly colorless, melts near body temperature, is very unreactive and is not water-soluble, all desirable properties for a cosmetic component. It would become a staple in cosmetics, including mascara. Soon after Vaseline became popular in the early 1900s another chemist and perfumer named Eugene Rimmel mixed black coal dust pigment with Vaseline to produce what is recognized as the first non-toxic mascara. It was also the first packaged mascara. At about the same time a second chemist T.L. Williams prepared a similar product for his sister Maybel. The product eventually became known as Maybelline (Maybel and Vaseline). Still, using mascara was a messy process, and continued so for forty years until Helena Rubenstein developed a tube containing mascara with a brush applicator inserted directly into the tube. The company also invented waterproof mascara. These two developments led to mascara becoming popular. It turns out that it is possible to actually grow longer eyelashes and not just lengthen them using mascara. Heres how. A drug called bimatoprost, a synthetic derivative of prostaglandin (left), is used to treat glaucoma but has as a side effect that it causes lashes to grow longer. The effect was discovered when the drug manufacturer Allergan tested the glaucoma medicine Lumigan, the prime component of which is bimatoprost. It increased the length of eyelashes and made them thicker. So Allergan obtained approval from the U.S. Food and Drug Administration to prescribe Lumigan for growing eyelashes, especially in people who had lost their lashes due to disease. The drug is now being tested as a treatment for baldness. More on mascara and cancer  The article says that the esters of para-hydroxybenzoic acid (parabens) have been used as preservatives in mascara in order to keep the product free of bacteria, fungi and molds. Parabens are also part of the formulations of shampoos, moisturizers, shaving gels and toothpaste. However, as the article also says, parabens have been found in breast cancer tissue and can also mimic the behavior of estrogen, a known factor in breast cancer. On the other hand, there has been no definitive link between parabens and cancer. Nevertheless, many cosmetic companies have removed them from their mascara products.  The parabens parent compound, 4-hydroxybenzoic acid (para-hydroxybenzoic acid) is derived from benzoic acid (left). A hydroxyl group is added to benzoic acid and then other functional groups are added in the esterification process to make any of the paraben compounds. The methyl group is most often the R group in the ester. This compound would be named methylparaben. Others in the series would be ethylparaben, propylparaben, etc. The U.S. Food and Drug Administration, which has jurisdiction over cosmetic products like mascara, issued this statement about parabens: What are parabens? Parabens are the most widely used preservatives in cosmetic products. Chemically, parabens are esters of p-hydroxybenzoic acid. The most common parabens used in cosmetic products are methylparaben, propylparaben, and butylparaben. Typically, more than one paraben is used in a product, and they are often used in combination with other types of preservatives to provide preservation against a broad range of microorganisms. The use of mixtures of parabens allows the use of lower levels while increasing preservative activity. Why are preservatives used in cosmetics? Preservatives may be used in cosmetics to protect them against microbial growth, both to protect consumers and to maintain product integrity. What kinds of products contain parabens? They are used in a wide variety of cosmetics, as well as foods and drugs. Cosmetics that may contain parabens include makeup, moisturizers, hair care products, and shaving products, among others. Most major brands of deodorants and antiperspirants do not currently contain parabens. Cosmetics sold on a retail basis to consumers are required by law to declare ingredients on the label. This is important information for consumers who want to determine whether a product contains an ingredient they wish to avoid. Parabens are usually easy to identify by name, such as methylparaben, propylparaben, butylparaben, or benzylparaben. Does FDA regulate the use of preservatives in cosmetics? The  HYPERLINK "http://www.fda.gov/RegulatoryInformation/Legislation/FederalFoodDrugandCosmeticActFDCAct/default.htm" Federal Food, Drug, and Cosmetic Act (FD&C Act) does not authorize FDA to approve cosmetic ingredients, with the exception of color additives that are not coal-tar hair dyes. In general, cosmetic manufacturers may use any ingredient they choose, except for a few ingredients that are prohibited by regulation. However, it is against the law to market a cosmetic in interstate commerce if it is adulterated. Under the FD&C Act, a cosmetic is adulterated if, among other reasons, it bears or contains any poisonous or deleterious substance which may render it injurious under the labeled conditions of use, or under customary or usual conditions of use. For more on this subject, see  HYPERLINK "http://www.fda.gov/Cosmetics/GuidanceComplianceRegulatoryInformation/ucm074162.htm" FDA Authority Over Cosmetics and  HYPERLINK "http://www.fda.gov/Cosmetics/GuidanceComplianceRegulatoryInformation/ucm074248.htm" Key Legal Concepts: "Interstate Commerce," "Adulterated," and "Misbranded. HYPERLINK "http://www.fda.gov/cosmetics/productandingredientsafety/selectedcosmeticingredients/cos-key.htm" " Are there health risks associated with the use of parabens in cosmetics? The Cosmetic Ingredient Review (CIR) reviewed the safety of methylparaben, propylparaben, and butylparaben in 1984 and concluded they were safe for use in cosmetic products at levels up to 25%. Typically parabens are used at levels ranging from 0.01 to 0.3%. On November 14, 2003, the CIR began the process to reopen the safety assessments of methylparaben, ethylparaben, propylparaben, and butylparaben in order to offer interested parties an opportunity to submit new data for consideration. In September 2005, the CIR decided to re-open the safety assessment for parabens to request exposure estimates and a risk assessment for cosmetic uses. In December 2005, after considering the margins of safety for exposure to women and infants, the Panel determined that there was no need to change its original conclusion that parabens are safe as used in cosmetics. (The CIR is an industry-sponsored organization that reviews cosmetic ingredient safety and publishes its results in open, peer-reviewed literature. FDA participates in the CIR in a non-voting capacity.) A study published in 2004 (Darbre, in the Journal of Applied Toxicology) detected parabens in breast tumors. The study also discussed this information in the context of the weak estrogen-like properties of parabens and the influence of estrogen on breast cancer. However, the study left several questions unanswered. For example, the study did not show that parabens cause cancer, or that they are harmful in any way, and the study did not look at possible paraben levels in normal tissue. FDA is aware that estrogenic activity in the body is associated with certain forms of breast cancer. Although parabens can act similarly to estrogen, they have been shown to have much less estrogenic activity than the bodys naturally occurring estrogen. For example, a 1998 study (Routledge et al., in Toxicology and Applied Pharmacology) found that the most potent paraben tested in the study, butylparaben, showed from 10,000- to 100,000-fold less activity than naturally occurring estradiol (a form of estrogen). Further, parabens are used at very low levels in cosmetics. In a review of the estrogenic activity of parabens, (Golden et al., in Critical Reviews in Toxicology, 2005) the author concluded that based on maximum daily exposure estimates, it was implausible that parabens could increase the risk associated with exposure to estrogenic chemicals. FDA believes that at the present time there is no reason for consumers to be concerned about the use of cosmetics containing parabens. However, the agency will continue to evaluate new data in this area. If FDA determines that a health hazard exists, the agency will advise the industry and the public, and will consider its legal options under the authority of the FD&C Act in protecting the health and welfare of consumers.  HYPERLINK "http://www.fda.gov/cosmetics/productandingredientsafety/selectedcosmeticingredients/ucm128042.htm" http://www.fda.gov/cosmetics/productandingredientsafety/selectedcosmeticingredients/ucm128042.htm Connections to Chemistry Concepts (for correlation to course curriculum) Organic compoundsMany of the chemicals that make up mascara are organic in nature. You can preview or review organic nomenclature and theory using these compounds as examples. Organic functional groupsOrganic functional groups are noted in the article, especially in the section on parabens. These are basic components of organic compounds and can be stressed here. Types of mixturesMany brands of mascara are actually emulsions. This gives you an opportunity to review miscible liquids, like dissolves like and polar and non-polar molecules. MixturesAlthough mascaras are mixtures of compounds, each one has a specific formula or ratio of components. Nonetheless, you can review mixtures using this article. EmulsionsEmulsions, one type of mixture rarely stressed in high school chemistry, can be described and explained using the article. Chemistry and diseaseThe section of the article on parabens provides an opportunity to discuss with your class the role that chemistry plays in diseases. Chemistry and historyGiven the section of the article about the history of mascara, you can discuss with students the way in which the use of chemical substances has changed and evolved throughout history. Possible Student Misconceptions (to aid teacher in addressing misconceptions) Eyelashes are very different from hair found on a persons head. Not true. Both have the same structure including follicles and shafts. Both shafts are made of keratin. Anticipating Student Questions (answers to questions students might ask in class) The article mentions cosmetic chemist. What is a cosmetic chemist? Cosmetic chemists or scientists can be found in cosmetic development, formulation, product and ingredient testing, quality control, analytical chemistry, process engineering, and working as synthesis chemists. Backgrounds in cosmetic chemistry can also be used by regulatory scientists and even in the sales and marketing of cosmetics products. Some cosmetic chemists work with the raw materials of skin-care products and others work with creating the finished goods. See  HYPERLINK "http://chemistscorner.com/how-to-become-a-cosmetic-chemist/" http://chemistscorner.com/how-to-become-a-cosmetic-chemist/ What is guanine? Guanine, with a formula of C5H5N5O (see structure below), is one of the four base pairs in the DNA molecule. It always pairs with cytosine. The substance is derived from purine. Guanine crystals are rhombic platelets composed of multiple transparent layers. They have a high index of refraction that partially reflects and transmits light from layer to layer, thus producing a pearly luster. Guanine used in mascara is usually extracted from the scales of fish, as the article says. It was first isolated from bird droppings which are known as guano, the term also used to refer to bat droppings. Bat excrement also contains guanine and the confusion about the terms guano and guanine led to the misconception that mascara contains bat excrement. The article mentions lead chloride compounds discovered in the eye makeup of ancient Egyptians that helped the body prevent disease. Lead chlorides are toxic. How can they prevent disease? A study published in Analytical Chemistry, an ACS publication, described how French chemists analyzed the powdery residue from ancient Egyptian eye makeup and found several lead chlorides compoundsgalena and laurionite among them. They used a tiny electrode to study the effect of laurionite on a single human cell. They found that when exposed to the lead chloride the cell produced increased quantities of nitric oxide, which is known to trigger the immune system into action to prevent disease. So even though the lead chloride compounds are poisonous at higher concentrations, they were present in low enough concentrations in Egyptian eye makeup so as not to be toxic but able to help fight disease. What is an emollient? See More on mascara for a discussion of emollients. In-class Activities (lesson ideas, including labs & demonstrations) Individual students or teams of students can collect mascara samples and test them for water solubility, ease of smearing and other properties identified by students. This can be an excellent way for you to have students design an experiment, record and organize data and draw conclusions based on this data. This activity can also be done outside of class. This lab activity illustrates polar solvents vs. non-polar solvents in several ways. ( HYPERLINK "http://www.cmu.edu/gipse/materials/pdf-2003/10-12/dissolve-M_Gelnett.pdf" http://www.cmu.edu/gipse/materials/pdf-2003/10-12/dissolve-M_Gelnett.pdf) This can be used to teach students about the mascara components that have polar molecules vs. those with non-polar molecules. This procedure has two partsmaking a hand cream emulsion and making mayonnaise. ( HYPERLINK "http://suite101.com/article/hair-cream-and-mayonnaise-making-emulsions-in-the-classroom-a265281" http://suite101.com/article/hair-cream-and-mayonnaise-making-emulsions-in-the-classroom-a265281) From Education World, this is the script for a demonstration about making an oil and water emulsion. ( HYPERLINK "http://www.educationworld.com/a_lesson/showbiz_science/showbiz_science013.shtml" http://www.educationworld.com/a_lesson/showbiz_science/showbiz_science013.shtml) This site has a procedure for making an emulsion, mayonnaise:  HYPERLINK "http://mypages.iit.edu/~smile/ch9509.html" http://mypages.iit.edu/~smile/ch9509.html. The Learning Channel has an emulsion-making activity at  HYPERLINK "http://tlc.howstuffworks.com/family/science-projects-for-kids-chemical-reactions11.htm" http://tlc.howstuffworks.com/family/science-projects-for-kids-chemical-reactions11.htm. This Web site has a complete description of emulsions and a series of lab activities that look at the factors involved with emulsions. (HYPERLINK "http://www.sciencenc.com/tournament-information/Event_rules_nc/2010%20NC%20Events/2010%20Food%20Science%20rules%20C.pdf"http://www.sciencenc.com/tournament-information/Event_rules_nc/2010%20NC%20Events/2010%20Food%20Science%20rules%20C.pdf) Out-of-class Activities and Projects (student research, class projects) Individual students or teams of students can collect mascara samples and test them for water solubility, ease of smearing and other properties identified by students. This can be an excellent way for you to have students design an experiment, record and organize data and draw conclusions based on this data. This activity can also be done in class. The pigment in mascara is important. This article describes substances used as mascara pigments throughout history. Students can use this Web site to investigate other inorganic pigments. ( HYPERLINK "http://artnc.org/lessons/elements-compounds-and-pigments" http://artnc.org/lessons/elements-compounds-and-pigments) Students can take photographs of the eyes of female classmates with and without mascara and compare the photos. Female members of the class can make instructional videos on how to apply mascara and the class can vote on the best one. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  Brownlee, C. Four Cool Chemistry Jobs. ChemMatters 2003, 21 (4) pp 1213. One of the jobs described in the article is that of a cosmetic chemist. Fruen, L. Cleopatras Perfume Factory and day Spa. ChemMatters 2004, 22 (3), pp 1315. The author describes cosmetics used and developed by Egyptian queen Cleopatra in ancient times, including kohl as described in the current article. Web sites for Additional Information (Web-based information sources) More sites on mascara This site has a straightforward outline of information about mascara, including its composition, manufacture and brief history:  HYPERLINK "http://www.enotes.com/mascara-reference/mascara" http://www.enotes.com/mascara-reference/mascara. How Stuff Works has a complete Web site on the Chemistry of Cosmetics with information that can be applied to mascara. ( HYPERLINK "http://science.howstuffworks.com/the-chemistry-of-cosmetics-info.htm" http://science.howstuffworks.com/the-chemistry-of-cosmetics-info.htm) This cosmetics chemistry Web site explains the functions of components of cosmetics like mascara:  HYPERLINK "http://www.chemistryexplained.com/Co-Di/Cosmetic-Chemistry.html" http://www.chemistryexplained.com/Co-Di/Cosmetic-Chemistry.html. National Institutes of Health has assembled a database of ingredients for common household materials ( HYPERLINK "http://householdproducts.nlm.nih.gov/index.htm" http://householdproducts.nlm.nih.gov/index.htm), including mascara:  HYPERLINK "http://householdproducts.nlm.nih.gov/cgi-bin/household/prodtree?prodcat=Personal+Care&purpose=Eye+Care/Makeup&type=mascara" http://householdproducts.nlm.nih.gov/cgi-bin/household/prodtree?prodcat=Personal+Care&purpose=Eye+Care/Makeup&type=mascara. More sites on the history of mascara An Egyptian travel bureau gives this history of eye makeup, here:  HYPERLINK "http://www.touregypt.net/egypt-info/magazine-mag09012000-mag4.htm" http://www.touregypt.net/egypt-info/magazine-mag09012000-mag4.htm. A trade group, CosmeticsINFO.org provides a time line for the development of cosmetics, including mascara. ( HYPERLINK "http://cosmeticsinfo.org/history2.php" http://cosmeticsinfo.org/history2.php) The Vine, a popular culture Web site, has a history of mascara and other eye cosmetics. ( HYPERLINK "http://www.thevine.com.au/fashion/beauty/the-history-of-mascara/" http://www.thevine.com.au/fashion/beauty/the-history-of-mascara/) More sites on emulsions From the University of Vancouver, Canada, comes an introduction to emulsions at  HYPERLINK "http://www.wasanlab.com/pharm/emulsion.html" http://www.wasanlab.com/pharm/emulsion.html. More sites on parabens The Center for Disease Control issued this fact sheet for parabens:  HYPERLINK "http://www.cdc.gov/biomonitoring/Parabens_FactSheet.html" http://www.cdc.gov/biomonitoring/Parabens_FactSheet.html. The U.S. Food and Drug Administration issued this statement on parabens:  HYPERLINK "http://www.fda.gov/cosmetics/productandingredientsafety/selectedcosmeticingredients/ucm128042.htm" http://www.fda.gov/cosmetics/productandingredientsafety/selectedcosmeticingredients/ucm128042.htm Dirty Business: Laundry Comes Clean with Chemistry Background Information (teacher information) More on the history of detergents Synthetic detergents were first developed in Germany in 1916, partly in response to the shortage of fats being used for the war effort (World War I). The surfactant, marketed as Nekal, was not used much for cleaning clothes, even though it was slightly better at cleaning than soaps of the time (cost may have been a factor here); however, it was a very good wetting agent, and as such it was used extensively (and still is) in the dye and textile industries for that purpose. Not much happened with detergents for several decades. In the 1930s, detergents were produced in the United States, but they didnt sell well (probably because they didnt clean well at that time). As we now know, surfactants by themselves have limited usefulness in cleaning; builders, enzymes, etc. are needed to make surfactants work effectively and efficiently as detergents. It wasnt until World War II that shortages of animal fats (used to produce materials for the war effort) once again forced manufacturers to research the development of cleaning agents that could be synthesized from raw materials not essential for the war effort, and that could be used by soldiers to clean clothes in sea-water (lots of minerals that made it hard water) and in cold water. Another driving force behind research into new cleaning agents was the fact that cleaning with soap, especially in hard water, resulted in dingy looking clothes due to the residual accumulation of soap scum, called soap curd, on clothes. It was hoped that detergents could solve this problem. The first commercial detergent produced in the US was Dreft, made by Proctor and Gamble as early as 1933. Unfortunately, with detergent research still in its infancy, that product only worked at cleaning clothing with light or no stains, and in light loads. (Today it is still produced, but primarily used for baby clothing or for fine washables; e.g., silks or wools, because it is such a mild cleaning agent.) Until World War II, about the only cleaning material available for washing laundry was soap. Housewives (not being sexist herebut at that time, housewives pretty much did all the laundry) had gotten used to shaving soap to make small shavings or flakes to put into the wash water. Smaller pieces were needed because whole bars of even smaller pieces of soap wouldnt completely dissolve in the wash water, and would thus not be effective at cleaning. (This would be a good time to discuss with students the effect of particle size on reaction rate.) Proctor and Gamble had realized the usefulness of soap flakes in saving women time at home, and by 1910 they had manufactured Ivory Flakes as a prepared laundry soap. Apparently, P&G wasnt the first to produce soap flakes (that honor goes to Lever Brothers in 1889 in Europe and imported into the US in 1906), but P&G may have been the first company to actively advertise and promote their flakes product. In 1943, Proctor and Gamble created their then-brand-new miracle laundry cleaner Tide. This was the first time that a surfactant had been formulated to include builders, the materials that helped surfactant penetrate more deeply into fabrics, increasing their stain-removing powers. After much laboratory testing, the product was released to the public in 1946. It was such a success with housewives that it became the best-selling laundry cleaner within two weeks of its release, and it frequently sold out in stores and at first had to be rationed, until production could be ramped up. To give an idea of the enormous rise in synthetic detergent production, Table 1 compiled from figures submitted by the American Soap and Detergent Association and the German firm of Henkel & Cie shows both soap and detergent sales in the USA for various years to 1972. Table 1 US Soap and Detergent Sales YearSoap Sales (1000 tons)Synthetic Sales (1000 tons)194014104.5195013406551960583164519725874448These figures reveal that immediately after the Second World War synthetics started making inroads into the production of soap, which now seems to have settled down to a constant whereas synthetics have increased enormously. By 1959 although the US per capita consumption had somewhat levelled out, total production was still rising as shown in Table 2 which has been compiled from the 1963 Census of Manufacturers by the Bureau of Census of the US Department of Commerce and from the Henkel figures. Table 2 Comparative Production Figures for Synthetic Detergents 1958 (1000 tons) 1963 (1000 tons) 1972 (1000 tons)Domestic detergents (solid) 1200 1425 2672Domestic detergents (liquid) 354 640 1773The broad picture that appears from Table 2, is that while solid detergents (among which of course powders are included) are making great strides forward, the liquid detergents are increasing at a much faster rate. ( HYPERLINK "http://chemistry.co.nz/deterghistorypart3.htm" http://chemistry.co.nz/deterghistorypart3.htm) More on surface tension  Here is a very simple description of the cause of surface tension. The molecule in the centre of a beaker of water is very strongly attracted to all of its immediate neighbours, [due to the polar nature of water molecules and hydrogen bonding] and the pull is equal in all directions. The molecule on the surface, however, does not have any neighbours to speak of, in the air or gas phase above. It therefore, is being pulled inward. The result is a force applied across the surface like the skin pulled over a drum. The effect is defined as surface tension. (See Figure One). The related effect is that the water tends to seek the minimum surface area per unit of volume, or tends to form Figure One spheres of droplets. Schematic Sketch of ( HYPERLINK "http://chemistry.co.nz/propwat.htm" http://chemistry.co.nz/propwat.htm) Surface Tension More on detergents Laundry detergents are a mixture of many materials, and typically contain surfactants, builders, whiteners, brighteners, perfumes and dyes, anti-re-deposition agents, enzymes and fillers. Each of these is described in a paragraph that follows. Surfactants, short for surface active agents, interact with both water and oil/grease. They lift stains from fabrics by attracting nonpolar oil particles and forming micelles. These are then rinsed out of the wash water. Surfactants make up somewhere between 8 and 18% of the detergent. Sodium lauryl sulfate (SLS, CH3(CH2)11OSO3Na) is a typical laundry anionic surfactant. Another large-scale component of laundry detergents is sodium dodecylbenzenesulfonate (C12H25C6H4SO3Na), a series of organic compounds (isomers). See More on surfactants below for more information on surfactants. Builders are essentially water softeners, substances added to detergent so that it works better in hard water conditions, where metallic cations such as Ca2+ and Mg2+ could form insoluble compounds that might re-precipitate back onto clothing, or that could react with the surfactants, thus reducing the efficiency of the detergent. Builders like phosphate can react with and remove these cations from the wash water (thus acting as water softeners in the wash cycle). Such compounds are among a group of substances called chelating agents, as they bind preferentially to di-cations (in this case, Ca2+ and Mg2+, which cause hard water). Compounds containing phosphate that once were prevalent in laundry detergents are sodium triphosphate (STP) or sodium tripolyphosphate (STTP). See More on phosphates below for information describing the demise of phosphates in laundry detergent. Other builders include sodium carbonate (washing soda), sodium silicate and borax (sodium tetraborate). In addition to being water softeners, builders are also basic, so they can neutralize acids in the system and aid the breakup of fat and oil molecules by rupturing their chemical bonds. They make surfactants more efficient, so manufacturers can use less surfactant in the mix. Almost half of the weight of a box of detergent can come from builders (typically 2045%). (Builders are cheaper than surfactants.) Whiteners are added to help make clothes appear whiter. Bleaches are the most common substances added to serve as whiteners. A drawback to using bleach is that most bleaches contain peroxides, which can also oxidize the fabric. While bleaches make stains colorless, they do not remove the stain, so you still need detergent if you want to wash away the stain. Bleach may comprise 1530% of the detergent. Typical bleaching agents are sodium percarbonate or sodium perborate. This equation describes the reaction of sodium percarbonate to produce hydrogen peroxide: H2O 2 Na2CO3" 3H2O2 (s) ! 2 Na2CO3 (aq) + 3 H2O2 (aq) Sodium percarbonate Sodium carbonate Hydrogen peroxide A very simplified equation describing the hydrolysis of perborate ions to produce hydrogen peroxide is shown here: BO31- (s) + 2 H2O (l) ! H2O2 (aq) + H2BO31- (aq) Perborate ion Water Hydrogen peroxide Borate ion  The more complete reaction involves not the single sodium perborate monohydrate molecule, NaBO3H2O, but its dimer, Na2H4B2O8, which looks like this: You can find more about this reaction at  HYPERLINK "http://www.organic-chemistry.org/chemicals/oxidations/sodiumperborate.shtm" http://www.organic-chemistry.org/chemicals/oxidations/sodiumperborate.shtm. Brighteners are added to minimize the yellowing of fabrics. These materials work by absorbing ultraviolet light (340370 nm range, UVA) and re-emitting it as visible blue light (typically 420470 nm) that masks yellowing by reflecting more blue light, making whites appear whiter, and thus brightening the fabric. Brighteners constitute about 0.1% of the detergent mix. Perfumes are added for obvious reasonsto ensure that the clothes we wear smell good after washing. (The fragrance could also mask odors not removed by the detergent.) In addition, the signature smell of a detergent could mean the difference between a manufacturer selling or not selling its product if consumers like/dont like the smell when they open the bottle of detergent. Dyes are added for the same reasonto make their product stand out from others on the market. The dye that colors the detergent is not concentrated enough to color fabrics washed in it, but there could be enough left on clothes after the wash cycle that it could be detectable by chemical/instrumental means. Anti-redeposition agents are used to ensure that stain materials removed from clothing in the wash do not re-deposit back onto the clothes later in the wash cycle. Both surfactants and builders can serve this purpose. Surfactants hydrophilic ends keep the dirt, attracted to the hydrophobic ends, moving through the wash water, preventing them from reattaching to the fabric, and builders react with the hard water cations, preventing them from reacting with stain molecules to form sticky precipitate molecules that would attach to clothes (and the inside of the washing machine) and remain there even through the rinse cycle. Where more anti-redeposition material is needed, carboxymethylcellulose or other similar water-soluble polymers can be added to the detergent. Enzymes are biological catalysts similar to those used in the body to digest food. (That shouldnt be surprising, since it is probably those same foods that have caused the stains on our clothing.) As mentioned in the article, the three types of enzymes in detergentsproteases (work on proteins), lipases (work on fats) and amylases (work on starches)each breaks down its own type of material. The most effective detergents will have all three types of enzymes (triple enzyme action) to help decompose the stains. Since enzymes are biological products that break down over time, detergent manufacturers must also add to the detergent mixture enzyme stabilizers that protect the enzymes and help them function. Enzymes comprise a bit less than 1% of the detergent (0.75%). Finally, fillers dilute and distribute the active ingredients so they are most effective. Powder and liquid detergents use different fillers. The principal ingredient in powder detergent is sodium sulfate. This gives the powdery, granular texture. Liquid detergents use water as the principal filler. Materials used as fillers for detergents include sodium sulfate, sodium chloride, borax, alcohols and anti-foaming agents. Fillers account for 5-45% of the total detergent mix. If the detergent is in liquid form, the filler is mostly water, at about 4-20%. ( HYPERLINK "http://home.howstuffworks.com/laundry-detergent.htm" http://home.howstuffworks.com/laundry-detergent.htm) The following is a list of some of the actual materials used by Proctor & Gamble in the production of their detergent line (in Europe): Surfactants anionic surfactants cationic surfactants nonionic surfactants Oxidizing Agents hydrogen peroxide peracids photo-oxidants Enzymes lipases amylases cellulases protease Softening Agents soaps zeolites silicates citrates Polymers polycarboxylates polyethylene glycols cellulose derivatives Other Ingredients buffers perfumes optical brighteners suds suppressors chelators (Proctor & Gambles UK Web site at  HYPERLINK "http://www.scienceinthebox.com/en_UK/research/detergentsprinciple_en.html" http://www.scienceinthebox.com/en_UK/research/detergentsprinciple_en.html) More on surfactants Essentially, there are four main types of surfactants, with the first three used the most in laundry detergents, and their actions depend on their interactions with ions. Ions are charged particles due to the gain or loss of electrons. Ions can be positive such as calcium, Ca2+, or negative such as chloride, Cl-. Anionic surfactants are negatively charged in solution. However, they do not work as well by themselves in hard water. This is because hard water has many positively charged ions presents such as calcium (Ca2+) and magnesium (Mg2+). Since anionic surfactants are negative they are attracted to the positive ions and bind, making them unable to bind to other molecules in solution. Nonionic surfactants have no charge. Therefore, they are not as easily impaired under hard water conditions, since they are not attracted to the positive ions. Cationic surfactants are positively charged in solution. They help the anionic surfactant molecules pack in at the water/dirt interface thereby allowing the anionic surfactants to pull more dirt away. Amphoteric or zwitterionic surfactants are both positively and negatively charged. These surfactants are very mild and are often found in gentler cleansers such as hand soaps, shampoos and cosmetics. [source:  HYPERLINK "http://home.howstuffworks.com/laundry-detergent6.htm" Silberberg]. (from howstuffworks:  HYPERLINK "http://home.howstuffworks.com/laundry-detergent1.htm" http://home.howstuffworks.com/laundry-detergent1.htm) As has been stated, a surfactant can reduce the surface tension of water significantly at quite low concentrations. An example of the extent of the reduction of surface tension in water/aqueous solution via the addition of a surfactant can be seen in the following table, showing that Softanol 90 [surfactantlinear secondary alcohols and ethoxylation products, Softanol 90used in detergent formulas] reduces the surface tension of water from 73 to 30 dynes per centimetre (mN/m) when used at a concentration of 0.005 percent. Ethanol when used at a concentration of 20 percent, however, only reduced tension of water to 38 dynes per centimetre. Relationship of Surface Tension and Concentration Table Two Percent Concentration required to reduce the surface tension of water to indicated values Surface tension, dynes per cm 73 50 40 30 22 Softanol 90 0 0.003 0.0008 0.0050 --- Ethanol 0 9 18 40 100 Here is a bit more detailed information about the nature of surfactant molecules and how they work. A particular type of molecular structure performs as a surfactant. This molecule is made up of a water soluble (hydrophilic) and a water insoluble (hydrophobic) component (Figure Two). The hydrophobe is usually the equivalent of an 8 to 18 carbon hydrocarbon, and can be aliphatic, aromatic, or a mixture of both. The sources of hydrophobes are normally natural fats and oils, petroleum fractions, relatively short synthetic polymers, or relatively high molecular weight synthetic alcohols.  The hydrophilic groups give the primary classification to surfactants, and are anionic, cationic and nonionic in nature. The anionic hydrophiles are the carboxylates (soaps), sulphates, sulphonates and phosphates. The cationic hydrophiles are some form of an amine product. The nonionic hydrophiles associate with water at the ether oxygens of a polyethylene glycol chain (Figure Three). In each case, the hydrophilic end of the surfactant is strongly attracted to the water molecules and the force of attraction between the hydrophobe and water is only slight. As a result, the surfactant molecules align themselves at the surface and internally so that the hydrophile end is toward the water and the hydrophobe is squeezed away from the water (Figure Four). This internal group of surfactant molecules is referred to as a micelle (m). Figure Four Schematic Sketch of Surfactant Molecules in Water ( HYPERLINK "http://chemistry.co.nz/surfactants.htm" http://chemistry.co.nz/surfactants.htm) Beyond their use in detergents, surfactants have many and varied uses. For example, they can be found in the following: facial creams (surfactant to remove oils from skin) contact lens cleaners (reduces surface tension and removes oilfrom tears) toothpaste (reduces surface tension, allowing toothpaste to enter crevices in teeth) paints (wetting agentreduces surface tensionto ensure more even flow of paint onto surface) shampoos (surfactants wash oil away from haircaused by sebum secreted from oil glands in scalp) dry-cleaning that uses liquid carbon dioxide as the solvent (relatively new process) More on optical brighteners Many organic materials (like fibers) tend to yellow with time, due to degradation by UV or visible light. The degraded molecules absorb blue light, resulting in the yellow appearance in daylight. Optical brighteners (really, fluorescent dyes), which absorb ultraviolet light and re-emit it in the blue range of the spectrum, make clothes appear brighter and thus counter this yellowing effect. A Web page at BASF describes in some detail how optical brighteners work. Actually, the detail involved in the absorption and fluorescence transitions may be more than you wantor not. It also describes the role of laundry bluing in making clothes appear whiter. (Laundry bluing was primarily used before optical brighteners came onto the scene, although it is still sold today.) Principles of optical brightening Optical brighteners are colorless or slightly colored organic compounds that, in solution or applied to a substrate, absorb ultraviolet light and re-emit most of it at between 400 and 500 nm as blue fluorescent light (Figure 1). Figure 1:Example absorption and fluorescence emission curves Solvent DMF Concentration 4.4 mg/l Layer thickness 1 cm Absorption maximum 375 nm Fluorescence maximum 437 nm Quantum yield 0.81   INCLUDEPICTURE "http://www.dispersions-pigments.basf.com/portal/streamer?fid=563287" \* MERGEFORMATINET  Figure 2 illustrates the processes involved in light absorption and fluorescence by optical brighteners. Absorption (A) of light quanta by the brightener molecules induces transition from the singlet ground state, S 0, to vibrational levels of the electronically excited singlet state, S 1. Brighteners in the S 1 state are deactivated by several routes. Fluorescence results from radiative transition to vibrational levels of the ground state (F). Deactivation processes competing with fluorescence are mainly non-radiative to the S 0 state (IC) and non-radiative to the triplet state (intersystem crossing, ISC). The efficiency of fluorescence is measured by the quantum yield : F = Number of quanta emitted Number of quanta absorbed Itis determined by the relative rates of fluorescence emission and the competing processes. When fixed in solid substrates, brighteners fluoresce with high quantum yields (ca. 0.9). Figure 2: Energy of optical brighteners and transitions A= absorption ISC = intersystem crossing F = fluorescenceS = singlet state IC = internal conversionT = triplet state  INCLUDEPICTURE "http://www.dispersions-pigments.basf.com/portal/streamer?fid=563289" \* MERGEFORMATINET  .. Materials that evenly reflect most of the light at all wavelengths striking their surface appear white to the human eye. Natural fibers, for example, generally absorb more light in the blue range of the visible spectrum (blue defect) than in others because of the impurities (natural pigments) they contain. As a result, natural fibers take on an unwanted, yellowish cast. Synthetic fibers also tend to yellow, although not as much (Figure 3).  INCLUDEPICTURE "http://www.dispersions-pigments.basf.com/portal/streamer?fid=563290" \* MERGEFORMATINET  Whiteness in substrates can be improved by (1) increasing reflection (reflectance) or (2) offsetting the blue defect. Bleaching does both of these to some extent, but invariably leaves behind part of the yellowish cast. Even the most thorough bleach cannot remove all traces of yellowing. Before the advent of optical brighteners, it was common practice to apply small amounts of blue or violet dyes (called bluing) to boost the visual impression of whiteness. These dyes absorb light in the green-yellow range of the spectrum, thereby reducing lightness. However, since they shift the shade of the yellowish material toward blue at the same time, the human eye perceives increased whiteness. Unlike dyes, optical brighteners offset the yellowish cast and at the same time improve lightness, because their bluing effect is not based on subtracting yellow-green light, but rather on adding blue light. Optical brighteners are virtually colorless compounds that, when present on a substrate, primarily absorb invisible ultraviolet light in the 300-400 nanometer (nm) range and re-emit it as visible violet-to-blue fluorescent light. This ability of optical brighteners to absorb invisible short-wave radiation and re-emit it in the visible blue light range, imparting brilliant whiteness to the light reflected by a substrate, is the key to their effectiveness. ( HYPERLINK "http://www.dispersions-pigments.basf.com/portal/basf/ien/dt.jsp?setCursor=1_564558" http://www.dispersions-pigments.basf.com/portal/basf/ien/dt.jsp?setCursor=1_564558) More on the role of enzymes Each of the three types of enzymes mentioned in the article is responsible for a different type of stain. By combining all three enzyme types, manufacturers can almost guarantee that the stains you encounter will be removed by its detergent formulation. The detergents enzyme combination may even result in a synergistic effect, as some stains contain more than one type of substance; e.g., a food stain may contain all threeprotein lipid and carbohydrate. Enzymes in detergent result in a cleaner wash and whiter white. In addition, they are said to be highly energy-efficient molecules, meaning that they can do their job of eliminating stains even in short wash cycles. And since these enzymes are effective at lower temperatures than the older bleaches, cold water detergents are becoming more prevalent. In addition to the role of stain removers in detergent, enzymes also act as brighteners and whiteners. Fibers, especially cotton, tend to degrade a bit with each wash, resulting in tiny fragments breaking and standing up and away from the fiber. These tiny fibers scatter light, making the fabric appear yellow. Enzymes attack the small strands and destroy them, smoothing out the fiber and reducing the amount of light they scatter, resulting in a whiter, brighter appearance. Heres a simple animation that illustrates the idea:  HYPERLINK "http://www.scienceinthebox.com/en_UK/glossary/popup_carezyme_en.html" http://www.scienceinthebox.com/en_UK/glossary/popup_carezyme_en.html. More on cold water detergents Detergents that work in cold water seem to work by having surfactant molecules that are shorter in chain length than typical surfactants. These organic molecules are more water soluble than their longer chain relatives. Unfortunately, they are not quite as good at dissolving oil and grease as their longer counterparts. But since they dissolve in cold water when the longer ones would not, they truly do function in cold water, which makes them better at cleaning in cold water than normal detergent surfactants. (Whether they clean at higher temperatures as well as normal detergents is not clear but, after all, consumers dont buy them to use them in hot water, now do they?) Tide Coldwater is specially designed with an increased amount of surfactant chemistry (as compared to regular Tide) that allows it to penetrate easily into fabrics. Tide Coldwater is also specially formulated with an increased amount of polymer technology (as compared to regular Tide) to suspend dirt particles to help prevent them from redepositing on fabrics, helping keep both your colors bright and your whites white. (from Tides official Web site,  HYPERLINK "http://www.tide.com/en-US/product/ultra-tide-coldwater-powder.jspx#info" http://www.tide.com/en-US/product/ultra-tide-coldwater-powder.jspx#info) Laundry bleaches like percarbonates and perborates are of little use in cold water detergent formulations, as they are not very soluble at lower temperatures and so, are not very effective in cold water washes. Using a hot water powder detergent formulation in a cold water wash will show cloudy water as the undissolved bleaches remain undissolved. Cold water detergents also use enzymes that activate at lower temperatures (but dont deteriorate at the higher temperatures of normal wash loads. These enzymes do some of the work of the bleaches that only work at higher temperatures. (See More on the role of enzymes, below.) Of course, the advantage of detergents that work in cold water is that they use much less energy (electricity) to run a load through a washing machine than detergents that require hot water. More on phosphatesout with the old  A substance that was formerly widespread as a builder (a water softener) in laundry detergent was sodium triphosphate (Na5P3O10). It is inexpensive and highly effective. The anion of this compound (P3O105) is strongly attracted to the positive ions naturally present in water (e.g., Ca2+, Mg2+ and Fe3+), because it has such a high negative charge. Despite its effectiveness, phosphate use was banned in laundry detergents in the United States in 1993, because its presence in wastewater was causing intense algae blooms in nearby lakes and streams. The phosphates serve as fertilizer for algae and other plant life in the water. When these algae die, they are decomposed by bacteria on the bottom of the bodies of water. In the process, the bacteria deplete the oxygen dissolved in the water, which causes the death of fish and other aquatic species due to the lack of oxygen. Consumers became so wary of phosphate-containing detergent products that even today, manufacturers detergent labels still say that the product contains no phosphates, even though detergents havent contained phosphates for almost 20 years. Phosphates are still used in dish detergents, but they are becoming illegal and are increasingly regulated in more and more states. As a result, some companies have replaced phosphates with more environmentally friendly substances in both laundry and dishwashing detergents. This led to consumer complaints for non-phosphate dish detergents that these new detergents did not clean dishes well and that glasses were encrusted with a hard white film. Research is still being done to ameliorate these problems.  Fortunately, good substitutes have been found for phosphates in laundry detergents, and these phosphate-free substitutes have proven to be effective in removing hard water ions. Such a substitute is ethylenediamine tetraacetic acid or EDTA for short. EDTA is a hexadentate ligand, meaning it has six teeth, six negative sites in its structure that can attach to a metal cation and sequester it, rendering it isolated from other ions in solutionthus resulting in softened water, where the surfactants can now work directly on laundry stains, rather than being used up by their interaction with the hard water ions. More on alkyl polyglycosides in with the new  A relatively new group of surfactants is the alkyl polyglycosides. Although they were discovered in the 1890s, they were of little practical use at that time, and they were difficult to prepare, so they were not used commercially until the 1990s when chemists discovered a way to produce them more easily. These are nonionic surfactants, made from plant-derived raw materials (renewable resources), are suitable for products that come in contact with human skin, and are biodegradable. They have been tested and found to be benign environmentally. These surfactants are used primarily in industrial and institutional laundry applications. They find more frequent commercial use in skin care products. They are being hailed as the ideal green surfactants. Several of the polyglycosides derive from alcohols from palm or coconut oils reacting with corn starch. If these surfactants are derived from glucose, they are known as alkyl polyglucosides (see structure above). You can read more about them here:  HYPERLINK "http://www.happi.com/articles/2008/04/apga-green-success-story" http://www.happi.com/articles/2008/04/apga-green-success-story. It might be interesting for students to compare the ingredients found in detergents back in 1985 (when the ChemMatters article, Detergents, was written) with the ingredients in detergents of today. The two tables below attempt to do just that. Table 1. Some common Ingredients found in detergent formulations NameFormulaAbbrev.Properties and UsesPhosphates Sodium tripolyphosphate Tetrasodium pyrophosphate Tetrapotassium pyrophosphate Trisodium phosphate Chlorinated trisodium phosphate Na5P3O10 Na4P2O7 K4P2O7 (Na3PO4 12 H2O)5 (Na3PO4 12 H2O)5 NaCIO STPP TSPP TKPP TSP CI-TSP Alkalinity builder, sequestering agent Same as STPP, slightly more alkaline Same as TSPP but used in liquid formulations because of higher solubility Gives very high alkalinity but no sequestering ability Gives high alkalinity and some sanitizing ability due to the release of hypochlorous acid (HCIO) Silicates Sodium metasilicate Sodium orthosilicate  Na2SiO3 5 H2O Na2SiO4 SMS SOS Provides alkalinity and is an anticorrosion agent due to its buffering ability In its anhydrous form, provides extremely high alkalinity to heavy-duty industrial formulations Carbonates Sodium carbonate Potassium carbonate  Na2CO3 K2CO3 Soda ash None Provides alkalinity and is a water softener Same as soda ash but in liquid formulations due to enhanced solubility Miscellaneous Triethanolamine Ethylenediamine tetraacetic acid, sodium salt Carboxymethylcellulose Xylenesulfonic acid, potassium salt  N(CH2CH2OH)3 C10H12O8N2Na4 -(C12H13O10Na3)n- C8H9SO3K  TEA EDTA CMC None Used for sequestering agent, primarily for iron(lIl) ion A strong sequestering agent used in liquid formulations Antiredeposition agent, thickener Hydrotope, that is, it enhances the solubility of other compounds in water (Wood, C. Detergents. ChemMatters 3 (2), pp 47) NameFormulaAbbrev.Properties and UsesSurfactantsAnionic Surfactants Linear alkyl sulfonates Sodium lauryl sulfate Diethanolamines Triethanolamines Alkyl ammonium chloride Nonionic Surfactants Alkyl phenoxy polyethoxy ethanols Alkyl polyglucosides  RSO2O1- (long linear alkyl group) CH3(CH2)11OSO3Na NH(CH2CH2OH)2 N(CH2CH2OH)3 C9H16Cl2N4 Varied See section above tables LAS SLS, SDS DEA TEA Quaternium 15 APGs Surfactantgood ability to keep particles dispersed in wash water Surfactant, inexpensive Surfactant, neutralize acids Emulsifier, used for sequestering agent, primarily for iron(lIl) ion Surfactant, disinfectant, deodorant Surfactant Natural, sugar-based surfactants, biodegradable, used primarily in industrial and institutional settingsBuildersSilicates Sodium metasilicate Sodium orthosilicate  Na2SiO3 5 H2O Na2SiO4 SMS SOS Provides alkalinity and is an anti- corrosion agent due to its buffering ability In its anhydrous form, provides extremely high alkalinity to heavy-duty industrial formulationsCarbonates Sodium carbonate Potassium carbonate  Na2CO3 K2CO3 Soda ash None Provides alkalinity and is a water softener Same as soda ash but in liquid formulas due to enhanced solubilityBorates Sodium tetraborate Na2B4O7 Borax Softens waterTriethanolamineN(CH2CH2OH)3 TEAEmulsifier, used for sequestering agent, primarily for iron(lIl) ionEnzymesProteases Lipases AmylasesAttack protein-based stains Attack fat/grease-based stains Attack carbohydrate-based stainsBleaching AgentsSodium percarbonate Sodium perborate2 Na2CO3 3 H2O NaBO3 4 H2OMiscellaneousEthylenediamine tetraacetic acid, sodium salt Carboxymethylcellulose Polyethylene glycol Xylenesulfonic acid, potassium saltC10H12O8N2Na4 -(C12H13O10Na3)n- EDTA CMC PEGA strong sequestering agent used in liquid formulations to chelate Ca2+, Mg2+ Antiredeposition agent, thickener Antiredeposition agent, regulates viscosity in liquid detergents Hydrotopeit enhances the solubility of other compounds in water (various sources) Connections to Chemistry Concepts (for correlation to course curriculum) Electron affinity/electronegativityOxygens higher electron affinity/electronegativity accounts for its ability to draw electrons away from hydrogen atoms toward itself in a water molecule, resulting in the polar nature of water. Hydrogen bondingWater owes its high surface tension to hydrogen bonding between water molecules. Surface tensionSurface tension must be overcome in order for cleaning agents to be able to lift non-polar molecules away from the fabric. Waters special propertiesWater has many unique properties. The main property focused on in this article is its high surface tension, due to its polar nature. Waters polarity explains many of its special properties; e.g., its role as universal solvent, high boiling and melting points, high heats of vaporization and fusion, high heat capacity, and solid water (ice) floating in its own liquid, caused by decrease of density due to bulk expansion upon freezing (also related to molecular structure of water). Polar moleculesWaters polar nature causes it to attract the polar ends of surfactant molecules, allowing the dirt molecules attracted to the nonpolar end of the surfactant to be washed out of the fabric and rinsed out of the washing machine. Non-polar moleculesNon-polar fat/oil/grease molecules are not attracted to water, so surfactant must be added to draw them out. Once attracted to the nonpolar end of the surfactant, they travel with the surfactant, which is attracted at its other, polar, end by polar water molecules and are rinsed out of the system. In addition, fat/oil/grease molecules can also dissolve in other non-polar solvents such as gasoline or perchloroethylene (used in dry cleaningdry, meaning, without water). Hydrophilic and hydrophobic (amphiphilic)Surfactants need both a hydrophilic part and a hydrophobic part in order to stay dissolved in water (hydrophilic) and also dissolve grease & oil (hydrophobic). Surfactants are thus amphiphilicattracted to both polar and nonpolar substances. CatalystsEnzymes are biological catalysts. One fact that was not mentioned in the article is that, since enzymes are not consumed in washing clothes, very little is needed to accomplish the task of removing stains in a wash cycle. This is an example of catalysis in students daily lives. Cations, anionsPhosphates triply-negative ion holds tightly to mineral cations dissolved/suspended in water and prevents those cations from re-depositing on clothing in the wash cycle. Anionic and cationic surfactants work because of their ionic structure. Double replacement reactionsMany of the builder molecules become involved with these reactions when they remove hard-water ions from the wash water; e.g., Ca2+, Mg2+. Examples: Washing soda as a water softener (with Ca2+ as hard water ion): CaSO4 (aq) + Na2CO3 (aq) ! CaCO3 (s) + Na2SO4 (aq) gypsum washing soda chalk sodium sulfate (found dissolved (added as water (precipitates (remains dissolved) in ground water) softener) out) Sodium triphosphate as a water softener (with Mg2+ as hard water ion): MgSO4 (aq) + Na5P3O10 (aq) ! Na2SO4 (aq) + MgP3O103- + 3Na+ Epsom salts sodium triphosphate sodium sulfate chelated Mg- sodium ions triphosphate ion (found dissolved (added as water (remains in (all of these remain in in ground water) softener) solution) solution and rinse out) Electromagnetic spectrumSince brighteners absorb ultraviolet light and re-emit in the blue portion of the visible spectrum, this would be a great topic to kick off discussion of the entire spectrum. It can also be a way to get into the discussion of energies associated with chemical reactions, since UV has higher energy than visible light. SolubilitySome of the water-softening reactions depend on the lower solubility of one of the products to remove the hard water ions from the water. Solubility & temperature dependenceMost laundry detergents work better in hot water than in cold water because of increased intermolecular motion. SurfactantsThis is a class of substances that have both a hydrophilic end, attracted to polar substances like water, and a hydrophobic end that is attracted to nonpolar substances like oils or fats. They are necessary in detergents to attract the dirt. Possible Student Misconceptions (to aid teacher in addressing misconceptions) Water is the only substance that has surface tension. Nope. Water may be the only substance students have ever heard of in the context of surface tension, but actually many substances exhibit surface tension. In fact, any liquid that forms beads or droplets is exhibiting surface tension. Mercury, for instance, forms beads even at extremely tiny sizes, indicating it has lots of surface tension (487 mN/m vs. waters 72 mN/m vs. gasolines 22 mN/m). ( HYPERLINK "http://en.wikipedia.org/wiki/Surface_tension" http://en.wikipedia.org/wiki/Surface_tension) Bleach removes stains. Actually, bleach only removes (or lightens) the color of the stain, not the stain itself; the stain molecules are still there. Thats why you still need to wash the stained article of clothing after the bleach has done its workor why some detergents contain bleach, to ensure that the stain will disappear even if some of its molecules remain after the wash cycle is complete. Ill just wash everything in hot water, that way I know it will be clean. OK, but you might regret it. Sure, the clothes will be clean, but they may also be damaged. Some fabrics need to be washed in warm wateror even cold waterto preserve the integrity of the fibers, to prevent shrinking or deforming of the clothes. Check the label to be sure youre doing it right. Once all that nanotechnology stuff comes to market, Ill never have to worry about washing clothes again. Nanomaterials have been used in clothing for a while now; e.g., nanoparticles of silver are used as an anti-germ, anti-odor additive to athletic socks, but were still washing even those clothes. At best, for now, nano-additives in clothes extend their use between washings, to minimize odor, stains, etc. But theyre not yet to the point of being truly protected from getting dirty, so they still need to be washedoccasionally Anticipating Student Questions (answers to questions students might ask in class) Are soap and detergent the same thing? Although they are both made for the purpose of cleaning, and they both have somewhat similar composition, their source and the mechanism of cleaning for each is very different. For more information, see these two ChemMatters articles: Soap (Wood, C. Soap. ChemMatters, 1985, 3 (1), pp 47) and Detergent (Wood, C. Detergents. ChemMatters, 1985, 3 (2), pp 47). Why are there so many different varieties of one brand of detergent? (Tide, for instance has 32 varieties.) One reason for many varieties has to do with consumer purchases, based on their preferences and needs: varieties based on scent will attract different consumers, and some consumers have allergies and need a dye- and perfume-free variety; some consumers may want their fabric softener to be included right in the detergent to save them time; some consumers need serious stain-removing capabilities in their detergent while others dont; consumers who are energy-conscious may choose the cold water formula to cut energy costs. A very real need (beyond consumer preferences) for differentiation exists for different types of washing machines that require different detergent typeshigh efficiency washers use less water so they need a detergent that produces fewer suds. And then one must consider the consumers environment: geographical areas that have soft water can use almost any garden-variety detergent, but areas with hard water will need specially formulated detergent made just for hard water environments. How does dry cleaning work? Dry cleaning uses a nonpolar solvent like perchloroethylene (or sometimes liquid carbon dioxide) to attract the oil/grease stains in clothing. The solvent itself then rinses the particles away from the fabric. It is called dry cleaning because no water is used, and the solvent molecules tend to stay on the surface of the fabric and dont penetrate the fibers the way water does. Do the enzymes used in detergent for stain removal come from living things? (Do we kill things to make them?) Although enzymes are biological catalysts, and therefore originated from living organisms, the enzymes are mass-produced in huge vats in production facilities, so no living things were harmed in the preparation of these enzymes. If fats are insoluble in water, how is it possible for fat to be absorbed into the blood stream since blood is primarily water? Fats, either saturated or unsaturated, are made soluble in blood by first dissolving in the digestive tract through the use of a biological detergent called bile, which is produced in the liver and stored in the gall bladder. The mixing (emulsifying) of the detergent with the fats allows for the non-polar end of the detergent to interact with the non-polar end of the fat (the fatty acid end) while the polar end of the detergent bonds with the polar end of the fat (the glyceride ends) just as regular soaps and detergents would do when you wash greasy dishes with soap or detergent. Once the fat is emulsified in the digestive juices, it can be broken apart by hydrolysis (water and enzymes) to yield water soluble fatty acids, glycerols, soaps or mono- and di-glycerides which are then small enough and soluble enough to pass through the intestinal wall into the blood stream, although it is not clear what form the digested fat takes in passing through the intestine. Lipids are found in the blood stream but are in the form of lipoproteins that are soluble. This is important because lipids are needed in various locations of the body including the liver and the brain. In-class Activities (lesson ideas, including labs & demonstrations) If you want to discuss with students why detergents are preferred over soap for use in hard water settings, you can discuss the chemistry of soaps interaction with hard water ions. This might tie in nicely with precipitation reactions, or solubility, or solubility equilibrium. Simple source material can be found here:  HYPERLINK "http://chemistry.about.com/od/cleanerchemistry/a/how-soap-cleans.htm" http://chemistry.about.com/od/cleanerchemistry/a/how-soap-cleans.htm. This Web site provides a series of experiments involving soap and detergent. In the first two experiments, students prepare their own soap and laundry detergent; in the third experiment they then test those two products for their: emulsifying properties, cleaning effectiveness in hard and soft water; alkalinity; and reaction with mineral acid. If you choose not to have students make their own soap and detergent, your students can still do the third experiment, using commercial soap and detergent. A student report sheet is provided. ( HYPERLINK "http://www.chymist.com/Soap%20and%20detergent.pdf" http://www.chymist.com/Soap%20and%20detergent.pdf) Heres another experiment, from Dave Brooks, that investigates the effectiveness of soap vs. that of detergent in hard water. It is followed by an experiment that shows how a precipitation reaction that softens the water helps soap clean. ( HYPERLINK "http://dwb.unl.edu/chemistry/labs/LABS12.html" http://dwb.unl.edu/chemistry/labs/LABS12.html) An interesting addition to the experiment in 2) above would be to investigate a third material, besides soap and laundry detergenta dishwashing detergent. The properties of this material would all match more closely to those of the tested detergent than to those of the soap, except possibly for their ability to produce suds. Dishwashing detergents are designed to produce few suds, so this would match the soaps property in terms of suds formation, but it would match the detergents property in terms of scum or curd formation. This experiment repeats the hard water dilemma with soap vs. detergent, but it takes the idea one step further by having students measure the hardness of the hard water. They do this by titrating the calcium ions in the hard water using EDTA. A student report form is included. ( HYPERLINK "http://www2.bakersfieldcollege.edu/deharvey/Laboratories/Lab%20Syllabus/Exp%2014%20Hard%20Water.pdf" http://www2.bakersfieldcollege.edu/deharvey/Laboratories/Lab%20Syllabus/Exp%2014%20Hard%20Water.pdf) To demonstrate the effect of hard water on cleaning effectiveness, you could set up two beakers, each containing a powdered laundry detergent solution (say, a teaspoon in 100 mL). Then using a dropper, add a few drops of salt (NaCl) solution to one beaker and Epsom salts (MgSO4) to the other. Nothing will happen with the salt, but precipitate will form when the magnesium ions react with the builder in the detergent. This indicates that some of the chemicals that would normally be used to clean the clothes are now being used to react with the hard water ions, thus decreasing the effectiveness of the detergent. Here is a short (4-page, with lots of photos, illustrations) pdf file that provides information about detergents and then asks a series of questions for students to answer. It is part of the Newfound/Laborador Education Consortium:  HYPERLINK "http://www.ed.gov.nl.ca/edu/k12/curriculum/guides/science/grade8/STSEScience8_fluids.pdf" http://www.ed.gov.nl.ca/edu/k12/curriculum/guides/science/grade8/STSEScience8_fluids.pdf. Since it is self-contained, you might try it as a lesson plan for a substitute teacher when you are out for a sick day. To show the effect of detergent on the surface tension of water, try the following. Fill a glass to the top with water and cover it securely with a piece of window screen. Cover the screen with a flat plate. Then invert the apparatus using the plate to hold the screen in place. When you carefully remove the plate, the screen will hold the water in the inverted glass. (You could discuss the role of air pressure in holding up the column of water at this point. Although that is not the focus of the demonstration, it helps to explain why the water stays up.) Return the glass to an upright position, add a little detergent, mix well, trying not to make bubbles, and replace the screen and plate. Reinvert the glass and remove the plate as before; the water now pours out. (Remember that the surfactant in the detergent acts to lower the surface tension of the water.) An interesting demonstration/experiment dealing with surface tension involves milk, food coloring and a drop of dish detergent. Observe what happens at  HYPERLINK "http://www.stevespanglerscience.com/experiment/milk-color-explosion" http://www.stevespanglerscience.com/experiment/milk-color-explosion. This makes a great inquiry-based experiment, especially if you dont tell students the science behind the effect. Another experiment that shows how detergent reduces surface tension involves putting a drop of detergent on the stern end of a paper cut-out boat and watching it move across still water. One version is experiment 9 at this site:  HYPERLINK "http://www.scienceinthebox.com/en_UK/main/HTML/Experiment-Sites_Green/HTML/pdf/Experiment_Menu.pdf" http://www.scienceinthebox.com/en_UK/main/HTML/Experiment-Sites_Green/HTML/pdf/Experiment_Menu.pdf. The pdf document from Proctor and Gamble contains all 16 experiments involving detergents. Heres another version of the detergent-driven boat that uses a plastic bag sealer. Theres a brief (1:13) video to show you how it works.  HYPERLINK "http://www.planet-science.com/categories/experiments/messy/2011/02/power-a-boat-with-soap.aspx" http://www.planet-science.com/categories/experiments/messy/2011/02/power-a-boat-with-soap.aspx Another experiment at the Proctor & Gamble site in #6 above involves a test to see which detergents contain enzymes. The activity uses a Petri dish containing agar gel to see which detergents can liquefy the gel. It is experiment 13 at this site:  HYPERLINK "http://www.scienceinthebox.com/en_UK/main/HTML/Experiment-Sites_Green/HTML/pdf/Experiment_Menu.pdf" http://www.scienceinthebox.com/en_UK/main/HTML/Experiment-Sites_Green/HTML/pdf/Experiment_Menu.pdf. Heres another experiment along the same vein, from GeorgiaStandards.Org:  HYPERLINK "https://www.georgiastandards.org/Frameworks/GSO%20Frameworks/9-12%20Science%20Block%20Biology%20Energy%20Transformations%20Enzyme%20Action%20of%20Detergents.pdf" https://www.georgiastandards.org/Frameworks/GSO%20Frameworks/9-12%20Science%20Block%20Biology%20Energy%20Transformations%20Enzyme%20Action%20of%20Detergents.pdf. This one includes standards, lesson plan, etc. You could demonstrate the alkalinity of detergents by testing with Universal indicator. This site provides an extensive series of student experiments and much background material on Surface Phenomenon and Colloids:  HYPERLINK "http://www.funsci.com/fun3_en/exper2/exper2.htm#presentation" http://www.funsci.com/fun3_en/exper2/exper2.htm#presentation. It includes discussions of surface tension, surfactants and detergents, and it includes Web references for many of the activities. This may be of more interest to biology teachers, but this pdf provides an experiment for students to perform in the field (well, really in the stream). Students can actually test for the presence of optical brighteners in local streams. Precautions are given to enhance safety. ( HYPERLINK "http://www.longwood.edu/cleanva/images/Sec5.opticalbrightlesson.pdf" http://www.longwood.edu/cleanva/images/Sec5.opticalbrightlesson.pdf) Here is a lesson plan that accompanies the pdf above:  HYPERLINK "http://www.eece.maine.edu/research/gk12/Optical_Brighteners.htm" http://www.eece.maine.edu/research/gk12/Optical_Brighteners.htm. This site from the Royal Society of Chemistry, UK, has a series of demonstrations involving fluorescence of common materials:  HYPERLINK "http://www.rsc.org/Education/EiC/issues/2006July/ExhibitionChemistry.asp" http://www.rsc.org/Education/EiC/issues/2006July/ExhibitionChemistry.asp. To add sun protection to clothing, some detergents contain UPF sun blocking material, most likely Tinosorb FD. Students can use a black light and UV beads to test the effectiveness of this sunblock on clothing laundered with the detergent. ( HYPERLINK "http://www.education.com/science-fair/article/laundry-detergent-uv-radiation/" http://www.education.com/science-fair/article/laundry-detergent-uv-radiation/) This experiment from the University of Maryland uses a spectrophotometer (you could perhaps use a colorimeter?) to measure the enzymatic action over time of a protease stain remover in laundry detergent as it removes a protein stain from cloth. ( HYPERLINK "http://www.eng.umd.edu/~nsw/ench485/lab2.htm#List" http://www.eng.umd.edu/~nsw/ench485/lab2.htm#List) If youre into typos, you might have students check out calcium hypocrite. (If a name brand laundry detergent gives you or other family members a rash, it may contain sodium or calcium hypocrite, alkyl benzene sodium sulfonate, or sodium tripolyphosphate.,  HYPERLINK "http://www.helium.com/items/1679867-green-chemistry-detergents" http://www.helium.com/items/1679867-green-chemistry-detergents) Maybe this detergent thinks its better than it really is? Out-of-class Activities and Projects (student research, class projects) Students might want to run their own tests on some detergents to assess the claims made in their advertisements. They will need to establish a standard testing regimen to ensure fair evaluations of all detergents. Students can form a research team to develop a methodical test of a laundry detergent. The will need to work out a protocol that will include control experiments so that their comparisons will be valid. The can test how much detergent remains in clothes, at the end of their wash cycle, that have been washed with the manufacturers recommended amount. This could be repeated after several washings with no new detergent added. Compare clothes washed with smaller amounts of detergent. What do your results say about Albert Donnays statement that People use too much detergent. Thats the dirty little secret of the detergent industry. (From the April 1997 ChemMatters Teachers Guide) The tests could include amount of sudsing or alkalinity from detergent left behind from each wash/rinse cycle, as well as brightness remaining on clothes (as seen under ultraviolet light, due to fluorescent additives). Students could compare the cleaning abilities of soaps vs. those of detergents, particularly in hard water. Again, they would need to establish standards of testing. Students can investigate labels on products such as detergents, soaps, shampoos and dishwashing detergents to determine the contents. They could then use reference books like the Merck Index to research each listed compound, find out its chemical structure and its properties, and finally, suggest its role in the optimal functioning of the product. Students could research and debate the use of phosphates in laundry and dishwashing detergents. Another area of interest (and some concern) related to soaps and detergents is the use of antibacterial soaps in hand-washing. Students could research the effects of washing hands with regular soap compared to those with antibacterial soap. Concern stems from possible bacterial resistance with continued use of anti-bacterial agents, as has happened with antibiotic overuse in both humans who are ill, and in livestock and poultry to prevent illness and spur growth. (Baxter, R. AntibacterialsFighting Infection Where it Lives. ChemMatters, 2002, 20 (3), pp 1011) (Sitzman, B., Goode, R. Hand Sanitizers, Soaps, and Antibacterial Agents: The Dirt on Getting Clean. ChemMatters, 2011, 29 (4), p 5) And here is a short About.com answer to the question Is anti-bacterial soap any better than regular soap? that students might use as a starting point for this project:  HYPERLINK "http://health.howstuffworks.com/skin-care/cleansing/myths/question692.htm" http://health.howstuffworks.com/skin-care/cleansing/myths/question692.htm. To test ingredients in detergents responsible for polluting local waterways, students could try something similar to this experiment. The activity involves measuring changes in small (2-L bottle) ecosystems over time. ( HYPERLINK "http://www.life.umd.edu/grad/mlfsc/labs/detergent.html#Notes" http://www.life.umd.edu/grad/mlfsc/labs/detergent.html) Students could investigate the effectiveness of a detergent containing a UV-absorbing compound in blocking UV-A radiation. Heres a place to start:  HYPERLINK "http://www.education.com/science-fair/article/laundry-detergent-uv-radiation/" http://www.education.com/science-fair/article/laundry-detergent-uv-radiation/. The site includes a few references. Students might be interested in determining the actual savings realized by using detergent that is designed to function in cold water vs. normal detergents. These cold water detergents usually cost more per load, but they save the user money because it takes much less electricity/gas to run cold water through the machine than to heat water for the warm/hot water load. Which detergent costs less overall remains to be determined. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  In this early ChemMatters article, author Wood describes the chemistry of soap, including the formation of soap micelles. (Wood, C. Soap. ChemMatters, 1985, 3 (1), pp 47) Heres another older article by the same author, this one about detergents. In this one, phosphates are still discussed as potential problems, saying State laws against phosphates must be considered. Students might find interesting the progress weve made in eliminating phosphates from detergents over these relatively few years. (Wood, C. Detergents. ChemMatters, 1985, 3 (2), pp 47) Students might be interested in reading about plastic laundry bags used in hospitals to contain soiled laundry that are thrown into the hot water wash right along with the laundry. The bags are made of a plastic, polyvinyl alcohol, that dissolves in hot water. The last page describes a student experiment using various solvents to test the solubility of polyvinyl alcohol plastic. (A sample of the plastic was provided with this issue of ChemMatters.) (Wood, C. Dissolving Plastic. ChemMatters, 1987, 5 (3), pp 1215) In the article, Old News, New Paper, ChemMatters discusses the role of surfactants and micelles in de-inking old newspaper to prepare it for recycling into new paper. (Borchardt, J. Old News, New Paper. ChemMatters, 1993, 11 (2), pp 1214) To save money, students might be tempted to use activated ceramic laundry disks or similar products that supposedly require no detergent at all! They can read more about them in this article: Goldfarb, B. Laundry Disks: Miracle or Money Down the Drain? ChemMatters, 1997, 15 (2), pp 1415. The December 1997 issue of ChemMatters presents a good description of hydrophobic and hydrophilic properties and of micelles as they relate to cleaning products, especially in hard water. (Dorrian, J. Dissolving Household Chores. ChemMatters, 1997, 15 (4), pp 1315) In this article author Rohrig discusses additives to detergents to increase their apparent brightness, and the role of fluorescence and its relationship to visible and ultraviolet light. (Rohrig, Brian. A Light of a Different Color. ChemMatters, 1999, 17 (2), pp 56) For a description of the process that uses super-critical carbon dioxide as the solvent for a dry cleaning process, see Kirchoff, M. A Supercritical Clean Machine. ChemMatters, 2000, 18 (2), pp 1415) This ChemMatters article discusses the use of anti-bacterial agents in hand-washingpros and cons. (Baxter, R. AntibacterialsFighting Infection Where it Lives. ChemMatters, 2002, 20 (3), pp 1011) To augment detergents in cleaning clothes, bleach is often used. Heres ChemMatters coverage of bleach. (Parent, K. Building a Better Bleach: A Green Chemistry Challenge. ChemMatters, 2004, 22 (2), pp 1719) In this ChemMatters article, author Halim introduces students to the world of nanotechnology. She discusses what nano is (materials from 1100 nm), the various forms it takes (tubes, wires, balls), applications (medicines, drug-delivery), and methods of fabrication (top-down, bottom-up) (Halim, N. Nanotechnologys Big Impact. ChemMatters, 2009, 27 (3), pp 1517) ( HYPERLINK "http://portal.acs.org/portal/PublicWebSite/education/resources/highschool/chemmatters/archive/CNBP_023539" http://portal.acs.org/portal/PublicWebSite/education/resources/highschool/chemmatters/archive/CNBP_023539) This ChemMatters article discusses whether we should regularly use anti-bacterial hand sanitizers to wash our hands. (Sitzman, B., Goode, R. Hand Sanitizers, Soaps, and Antibacterial Agents: The Dirt on Getting Clean. ChemMatters, 2011, 29 (4), p 5) ____________________ The Journal of Chemical Education contains the following article about the use of a flatbed scanner to determine detergent efficiency: Poce-Fatou, J.A.; Bethencourt, M.; Moreno-Dorado,F.J.; Palacios-Santander, J.M.; Using a Flatbed Scanner to Measure Detergency: A Cost-Effective Undergraduate Laboratory. Journal of Chemical Education, 2011 88, pp 13141317. (also available to J Chem Ed subscribers online at  HYPERLINK "http://pubs.acs.org/doi/pdf/10.1021/ed100635z" http://pubs.acs.org/doi/pdf/10.1021/ed100635z.) Web sites for Additional Information (Web-based information sources) More sites on soaps and detergents For a visual company history of Proctor & Gambles development, that began with the manufacture of candles and soaps and now includes many vastly different families of materials, from 1837 to 2005, visit their historical site at  HYPERLINK "http://www.pg.com/translations/history_pdf/english_history.pdf" http://www.pg.com/translations/history_pdf/english_history.pdf. This site,  HYPERLINK "http://inventors.about.com/library/inventors/blsoap.htm" http://inventors.about.com/library/inventors/blsoap.htm, gives a history about the commercial development of soaps, beginning back in the 19th century. Do you realize that liquid soap was first developed in 1865? And how did Ivory soap come to be the floating soap? For good illustrations, with equations and with color 3-D models of important molecules in the synthesis of soap and the various types of detergents, refer to the following Web sites from Elmhurst College Chemistry Department:  HYPERLINK "http://elmhcx9.elmhurst.edu/~chm/vchembook/554soap.html" http://elmhcx9.elmhurst.edu/~chm/vchembook/554soap.html and  HYPERLINK "http://elmhcx9.elmhurst.edu/~chm/vchembook/558detergent.html" http://elmhcx9.elmhurst.edu/~chm/vchembook/558detergent.html. For a different and less technical discussion of soap and detergent synthesis and the chemistry behind the reactions, refer to  HYPERLINK "http://www.cleaning101.com/sdalatest/html/soapchemistry1.htm" http://www.cleaning101.com/sdalatest/html/soapchemistry1.htm. Howstuffworks.com has a nice, concise 7-screen Web page describing how laundry detergent works. View it at ( HYPERLINK "http://home.howstuffworks.com/laundry-detergent.htm" http://home.howstuffworks.com/laundry-detergent.htm). The site also has a short 9-screen description involving the chemistry of cleaning clothes. View it at  HYPERLINK "http://science.howstuffworks.com/the-chemistry-of-cleaning-clothes-info.htm" http://science.howstuffworks.com/the-chemistry-of-cleaning-clothes-info.htm. More sites on detergents Proctor and Gamble provides an MSDS sheet for their laundry detergents. Here is the one for many varieties of liquid Tide detergents:  HYPERLINK "http://www.pg.com/productsafety/msds/fabric_and_homecare/detergents/Liquid_2X_TIde_Laundry_Detergent.pdf" http://www.pg.com/productsafety/msds/fabric_and_homecare/detergents/Liquid_2X_TIde_Laundry_Detergent.pdf. The MSDS does not list individual chemicals, but merely cites groups of chemicals. (The fact that the list comprises only 45% of the total composition of the detergent could lead one to surmise that the other 55% is water.) The MSDS for granular Tide found here  HYPERLINK "http://www.pg.com/productsafety/msds/fabric_and_homecare/detergents/Ultra_Tide_Granular_Laundry_Detergent.pdf" http://www.pg.com/productsafety/msds/fabric_and_homecare/detergents/Ultra_Tide_Granular_Laundry_Detergent.pdf has ingredients whose varying composition ranges could add up to 100%. Proctor and Gambles Web site also has a series of 16 experiments students can do using detergents. The experiments range from K12. You can find them here:  HYPERLINK "http://www.scienceinthebox.com/en_UK/main/HTML/Experiment-Sites_Green/HTML/pdf/Experiment_Menu.pdf" http://www.scienceinthebox.com/en_UK/main/HTML/Experiment-Sites_Green/HTML/pdf/Experiment_Menu.pdf. Proctor and Gamble presents a series of 43 slides describing their plan Sustainable Innovation (for detergents) by 2020. Some of the slides might need more explanation, but some might be useful to show where theyre headed in the future, especially with cold water detergents. It includes lots of tables and charts. ( HYPERLINK "http://www.dtsc.ca.gov/PollutionPrevention/GreenChemistryInitiative/upload/AAIII_7_Versteeg.pdf" http://www.dtsc.ca.gov/PollutionPrevention/GreenChemistryInitiative/upload/AAIII_7_Versteeg.pdf) The Web site Kiwi Web: Chemistry & New Zealand provides some detailed information about detergents: their history, production history, washing improvements, chemistry and environmental history. Go to  HYPERLINK "http://chemistry.co.nz/deterghistorypart2.htm" http://chemistry.co.nz/deterghistorypart2.htm and click on tabs under the Kiwi Web logo at the top of the page. And look around on each new page for more topics; the site is not always to navigate. You might actually want to start with this page,  HYPERLINK "http://chemistry.co.nz/propwat.htm" http://chemistry.co.nz/propwat.htm, and work your way forward.  HYPERLINK "http://www.happi.com/articles/2008/04/apga-green-success-story" http://www.happi.com/articles/2008/04/apga-green-success-story SteveSpanglerScience.com has a page on UV beads with suggestions for experiments students can do:  HYPERLINK "http://www.stevespanglerscience.com/experiment/uv-reactive-beads" http://www.stevespanglerscience.com/experiment/uv-reactive-beads. (You can also buy the beads at this site.) Pin Stripes and Polka Dots provides a rather extensive list of commercial detergents and their ingredients. The list is not terribly revealing, just the list of ingredients that are listed on the label; nonetheless, the list of brands (>60) is extensive. ( HYPERLINK "http://www.pinstripesandpolkadots.com/detergentchart.htm" http://www.pinstripesandpolkadots.com/detergentchart.htm) More sites on surface tension The AboutChemistry.com Web site utilized in the Background Information section contains a short synopsis of waters polar nature, and data on its molecular weight, boiling point and surface tension, relative to its periodic table relatives (H2S, NH3, etc.) that you might want to use in your classroom. View it at  HYPERLINK "http://chemistry.co.nz/propwat.htm" http://chemistry.co.nz/propwat.htm. More sites on surfactants The AboutChemistry.com Web site also has more detailed information about the various functions of surfactants at utilized in the Background Information section contains a short synopsis of waters polar nature, and data on its molecular weight, boiling point and surface tension, relative to its periodic table relatives (H2S, NH3, etc.) that you might want to use in your classroom. View it at  HYPERLINK "http://chemistry.co.nz/surfactants.htm" http://chemistry.co.nz/surfactants.htm. Here is a request for an exemption to FDA regulation for a series of alkyl polyglucoside surfactants, based on a GRAS (Generally Regarded As Safe) report:  HYPERLINK "http://www.accessdata.fda.gov/scripts/fcn/gras_notices/grn000237.pdf" http://www.accessdata.fda.gov/scripts/fcn/gras_notices/grn000237.pdf. The exemption was submitted by a consulting group to the FDA in 2007. More sites on enzymes Laundry DetergentHow Enzymes are Changing Your Wash is the topic of this site. It contains information about how the enzymes are chosen and mass-produced. ( HYPERLINK "http://www.housekeepingchannel.com/a_220-Laundry_Detergent___How_Enzymes_are_Changing_Your_Wash" http://www.housekeepingchannel.com/a_220-Laundry_Detergent___How_Enzymes_are_Changing_Your_Wash) The LiveStrong site offers this page that discusses several laundry detergents that use enzymes. View it at  HYPERLINK "http://www.livestrong.com/article/144673-laundry-detergents-that-use-enzymes/" http://www.livestrong.com/article/144673-laundry-detergents-that-use-enzymes/. Proctor and Gambles site contains a page on enzymeswhat they are and how they work in detergents:  HYPERLINK "http://www.scienceinthebox.com/en_UK/glossary/enzymes2_en.html" http://www.scienceinthebox.com/en_UK/glossary/enzymes2_en.html. More sites on phosphates and eutrophication One Web page of the US Geological Survey (USGS) Toxic Substances Hydrology Program Web site at  HYPERLINK "http://toxics.usgs.gov/definitions/eutrophication.html" http://toxics.usgs.gov/definitions/eutrophication.html provides several definitions of the term eutrophication and contains lots of links to other pages on their site that deal with phosphates and other nutrients that cause eutrophication. A 2-page (pdf) fact sheet from Cornell University describing the phosphorus cycle can be found here:  HYPERLINK "http://nmsp.cals.cornell.edu/publications/factsheets/factsheet12.pdf" http://nmsp.cals.cornell.edu/publications/factsheets/factsheet12.pdf. As it is published by the College of Agriculture and Life Sciences, it focuses on phosphorus build-up from runoff of fertilizers into bodies of fresh water. You can view the phosphorus cycle via an animation from Discover Biology, 3rd ed., published by W. W. Norton & Co., on the Web at  HYPERLINK "http://www.sumanasinc.com/webcontent/animations/content/phosphorouscycle.html" http://www.sumanasinc.com/webcontent/animations/content/phosphorouscycle.html. The animation can be used with or without narration. If the viewer chooses the step-through mode, text appears (without narration) to accompany the diagrams as the viewer can pace herself through the sequence; the narration runs at its own pace. A comprehensive discussion of the phosphate detergent conflict can be found here:  HYPERLINK "http://www.colorado.edu/conflict/full_text_search/AllCRCDocs/94-54.htm" http://www.colorado.edu/conflict/full_text_search/AllCRCDocs/94-54.htm. The article, Historical Perspective of the Phosphate Detergent Conflict provides just that, with more than 25 references (hard copy references, not Web sites). It covers only the time period from the early 1960s to 1993, so the more recent decisions by states to ban phosphate in dishwashing detergents are not covered. This article from Virginia Tech describes the detection of optical brighteners in local waters using a fluorometer. It also has a section that describes how optical brighteners work. It includes photos and diagrams, as well as references. ( HYPERLINK "http://filebox.vt.edu/users/chagedor/biol_4684/BST/OB%20Article-I.pdf" http://filebox.vt.edu/users/chagedor/biol_4684/BST/OB%20Article-I.pdf) More sites on soil and the chemistry of washing clothes Proctor and Gamble has a simple page that describes what laundry soil is and where it comes from. ( HYPERLINK "http://www.scienceinthebox.com/en_UK/research/originsofdirt_en.html" http://www.scienceinthebox.com/en_UK/research/originsofdirt_en.html) Dr. Chemical: Chemistry in the Real World is a blog that discusses many chemical topics as they relate to daily life. He discussed the topic of cleaning clothes and detergents in June, 2012. The site below starts at The Chemistry of Clothes Washing #1 on June 9 and continues through June 25, with episode #13. It is an interesting blog in general, not just for clothes laundering. ( HYPERLINK "http://drchemical.com.au/2012/06/page/4" http://drchemical.com.au/2012/06/page/4) More sites on new technology The picture in the article involving NeverWet superhydrophobic spray, the chocolate syrup and the sneaker doesnt do the product justice. Go to the NeverWet Web site to see a brief video that shows water and chocolate syrup shooting off the sneaker surface (which, by the way, is cloth, not leather or plasticso the syrup should sink in, not slide off). ( HYPERLINK "http://www.techeblog.com/index.php/tech-gadget/incredible-neverwet-superhydrophobic-spray-lets-chocolate-syrup-slide-right-off-your-shoes" http://www.techeblog.com/index.php/tech-gadget/incredible-neverwet-superhydrophobic-spray-lets-chocolate-syrup-slide-right-off-your-shoes) More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers) Coming Clean With Enzymes, a chapter in the Science in the Real World: Microbes in Action series, is a 4-lab, 4-day high school curriculum unit dealing with enzymes. These labs show the ability of bacteria to produce extracellular enzymes. They also demonstrate the ability of these enzymes, when produced and collected through biotechnological techniques, to function as additives to household detergents and cleaners. ( HYPERLINK "http://www.umsl.edu/~microbes/pdf/Coming%20Clean%20With%20Enzymes.pdf" http://www.umsl.edu/~microbes/pdf/Coming%20Clean%20With%20Enzymes.pdf)      PAGE \* MERGEFORMAT 2  PAGE \* MERGEFORMAT 1 PAGE  PAGE 90 PAGE  PAGE 112 The references below can be found on the ChemMatters 25-year CD (which includes all articles published during the years 1983 through 2008). The CD is available from ACS for $30 (or a site/school license is available for $105) at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://www.acs.org/chemmatters. (At the right of the screen, click on the ChemMatters CD image like the one at the right.) Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online at this same site. (Full ChemMatters articles and Teachers Guides are available on the 25-year CD for all past issues, up to 2008.) Some of the more recent articles (2002 forward) may also be available online at the URL listed above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the page. If the article is available online, you will find it there. The references below can be found on the ChemMatters 25-year CD (which includes all articles published during the years 1983 through 2008). The CD is available from ACS for $30 (or a site/school license is available for $105) at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://www.acs.org/chemmatters. (At the right of the screen, click on the ChemMatters CD image like the one at the right.) Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online at this same site. (Full ChemMatters articles and Teachers Guides are available on the 25-year CD for all past issues, up to 2008.) Some of the more recent articles (2002 forward) may also be available online at the URL listed above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the page. If the article is available online, you will find it there. The references below can be found on the ChemMatters 25-year CD (which includes all articles published during the years 1983 through 2008). The CD is available from ACS for $30 (or a site/school license is available for $105) at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://www.acs.org/chemmatters. (At the right of the screen, click on the ChemMatters CD image like the one at the right.) Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online at this same site. (Full ChemMatters articles and Teachers Guides are available on the 25-year CD for all past issues, up to 2008.) Some of the more recent articles (2002 forward) may also be available online at the URL listed above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the page. If the article is available online, you will find it there. 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The CD is available from ACS for $30 (or a site/school license is available for $105) at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://www.acs.org/chemmatters. (At the right of the screen, click on the ChemMatters CD image like the one at the right.) Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online at this same site. (Full ChemMatters articles and Teachers Guides are available on the 25-year CD for all past issues, up to 2008.) Some of the more recent articles (2002 forward) may also be available online at the URL listed above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the page. If the article is available online, you will find it there. Carboxymethylcellulose (Wikipedia)  Hydrophobe Hydrophile Figure Two Schematic Sketch of Surfactant Molecule Figure Three Polyethylene Glycol Chain  The chelation of a metal cation (M) by the triphosphate ion (Wikipedia)  Metal ion chelated by EDTA (Wikipedia)  Alkyl polyglucoside (Wikipedia) The references below can be found on the ChemMatters 25-year CD (which includes all articles published during the years 1983 through 2008). The CD is available from ACS for $30 (or a site/school license is available for $105) at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://www.acs.org/chemmatters. (At the right of the screen, click on the ChemMatters CD image like the one at the right.) Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online at this same site. (Full ChemMatters articles and Teachers Guides are available on the 25-year CD for all past issues, up to 2008.) Some of the more recent articles (2002 forward) may also be available online at the URL listed above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the page. 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