ࡱ>  13,-./0xz_ Ibjbj PjA\jA\6 - - B/B/B/$f/f/f/P/d2f/7=8>8>8>Qf$m$y/&B/qQ@Qqq--8>8>d :DvDvDvq-"8>B/8>DvqDvDv.r> 8>ydRqC<"ULr,UJUB/Jt.p"PpDvhp|p.p.p.pxt.p.p.pqqqqU.p.p.p.p.p.p.p.p.p > ^,:  Generic Small Commercial Passenger Vessel Quality Assurance Project Plan (QAPP) For SAMPLING AND ANALYSIS OF TREATED SEWAGE AND GRAYWATER December 6, 2015 Submitted to fulfill certain requirements of Alaska Statue 46.03.460- 46.03.490 and 19 AAC 69  Alaska Department of Environmental Conservation Division of Water A. PROJECT MANAGEMENT ELEMENTS A.1 Title and Approvals: Title: Generic Small Cruise Ship Quality Assurance Project Plan for Sampling and Analysis of Treated Blackwater and Graywater Name: Project Manager Phone: Organization Name: email:  HYPERLINK "mailto:richard.heffern@alaska.gov"  Signature: ______________________________ Date: ______________ Name: ADEC CPVEC Project Manager Phone: ADEC Program Commercial Passenger Vessel Env Compliance email:  HYPERLINK "mailto:richard.heffern@alaska.gov"  Signature: ______________________________ Date: ______________ Douglas Kolwaite, ADEC DOW QA Officer Phone: (907) 465-5305 ADEC DOW WQSAR Program email:  HYPERLINK "mailto:Douglas.kolwaite@alaska.gov" Douglas.kolwaite@alaska.gov Signature: ______________________________ Date: ______________ TABLE OF CONTENTS  TOC \o "1-3" \t "Tables,1" A.1 Title and Approvals:  PAGEREF _Toc436807529 \h 2 TABLE OF CONTENTS  PAGEREF _Toc436807530 \h 3 A.3 DISTRIBUTION LIST  PAGEREF _Toc436807531 \h 5 A.4 PROJECT TASK/ORGANIZATION  PAGEREF _Toc436807532 \h 5 A.5 PROBLEM DEFINITION/BACKGROUND AND PROJECT OBJECTIVES  PAGEREF _Toc436807533 \h 7 A.5.1Problem Definition  PAGEREF _Toc436807534 \h 7 A.5.2Project Background  PAGEREF _Toc436807535 \h 7 A.5.3Project Objective(s)  PAGEREF _Toc436807536 \h 8 A.6 PROJECT/TASK DESCRIPTION and SCHEDULE  PAGEREF _Toc436807537 \h 8 A.6.1Project Description  PAGEREF _Toc436807538 \h 8 A.7 DATA QUALITY OBJECTIES AND CRITERIA FOR MEASUREMENT DATA  PAGEREF _Toc436807539 \h 9 A.7.1 Data Quality Objectives (DQOs)  PAGEREF _Toc436807540 \h 9 A.7.2 Measurement Quality Objectives (MQOs)  PAGEREF _Toc436807541 \h 9 A.8 SPECIAL TRAINING REQUIREMENTS/CERTIFICATION  PAGEREF _Toc436807542 \h 17 A.9 DOCUMENTS AND RECORDS  PAGEREF _Toc436807543 \h 17 A.9.1 Sample schedule and Vessel/Sample Identification  PAGEREF _Toc436807544 \h 17 A.9.2 Field Records (Required for ADEC compliance samples)  PAGEREF _Toc436807545 \h 18 A.9.3 Laboratory Records  PAGEREF _Toc436807546 \h 18 A.9.4 Chain of Custody  PAGEREF _Toc436807547 \h 18 B. DATA GENERATION AND ACQUISITION  PAGEREF _Toc436807548 \h 19 B.1 SAMPLING PROCESS DESIGN  PAGEREF _Toc436807549 \h 19 B.2 SAMPLING METHOD REQUIREMENTS  PAGEREF _Toc436807550 \h 19 B.2.1 Sample Types  PAGEREF _Toc436807551 \h 20 B.2.2 Sample Containers and Equipment  PAGEREF _Toc436807552 \h 20 B.2.3 Sampling Methods  PAGEREF _Toc436807553 \h 22 B.3 SAMPLE HANDLING AND CUSTORY REQUIREMENTS  PAGEREF _Toc436807554 \h 23 B.3.1 Sampling Procedures  PAGEREF _Toc436807555 \h 23 B.3.2 Sample Custody Procedures  PAGEREF _Toc436807556 \h 23 B.3.3 Shipping Requirements  PAGEREF _Toc436807557 \h 24 B.4 ANALYTICAL METHODS AND REQUIREMENTS  PAGEREF _Toc436807558 \h 24 B.5 QUALITY CONTROL REQUIREMENTS  PAGEREF _Toc436807559 \h 25 B.5.1 Field Quality Control (QC) Measures  PAGEREF _Toc436807560 \h 25 B.5.2 Laboratory Quality Control (QC) Measures  PAGEREF _Toc436807561 \h 26 B.6 INSTRUMENT/EQUIPMENT TESTING, INSPECTIONAND MAINTENANCE REQUIREMENTS  PAGEREF _Toc436807562 \h 26 B.7 INSTRUMENT CALIBRATION AND FREQUENCY  PAGEREF _Toc436807563 \h 26 B.8 INSPECTION/ACCEPTANCE OF SUPPLIES AND CONSUMABLES  PAGEREF _Toc436807564 \h 27 B.9 DATA MANAGEMENT  PAGEREF _Toc436807565 \h 27 C.1 ASSESSMENTS AND RESPONSE ACTIONS  PAGEREF _Toc436807566 \h 28 C.1.1 Field Assessments  PAGEREF _Toc436807567 \h 28 C.1.2 Laboratory Assessments  PAGEREF _Toc436807568 \h 28 C.1.3 Replicates  PAGEREF _Toc436807569 \h 28 C.1.4 Corrective Action  PAGEREF _Toc436807570 \h 28 C.2 REVISIONS TO QAPP  PAGEREF _Toc436807571 \h 28 D. DATA VALIDATION AND USABILITY  PAGEREF _Toc436807572 \h 29 D.1 DATA REVIEW, VERIFICATIONAND VALIDATION REQUIREMENTS  PAGEREF _Toc436807573 \h 29 D1.1 Data validation  PAGEREF _Toc436807574 \h 29 D1.2 Data Verification  PAGEREF _Toc436807575 \h 29 D1.3 Data Review  PAGEREF _Toc436807576 \h 29 D.2 VERIFICATION AND VALIDATION METHODS  PAGEREF _Toc436807577 \h 30 D2.1 Validation Methods  PAGEREF _Toc436807578 \h 30 D2.2 Verification Methods  PAGEREF _Toc436807579 \h 30 D.3 RECONCILIATION WITH USER REQUIREMENTS  PAGEREF _Toc436807580 \h 30 Appendix A - Alaska Small Cruise Ship Sampling Checklist  PAGEREF _Toc436807581 \h 31 Notification:  PAGEREF _Toc436807582 \h 31 Type of Sampling  PAGEREF _Toc436807583 \h 31 Ready Equipment  PAGEREF _Toc436807584 \h 31 Check that equipment operates, and that calibration is up-to-date  PAGEREF _Toc436807585 \h 31 Protective Equipment- check condition and availability  PAGEREF _Toc436807586 \h 31 Sampling Bottles  PAGEREF _Toc436807587 \h 31 Verify Sampling Site  PAGEREF _Toc436807588 \h 32 Review VSSP and BMP  PAGEREF _Toc436807589 \h 32 Pre sampling brief with vessel Master  PAGEREF _Toc436807590 \h 32 Sampling Field Notes will include:  PAGEREF _Toc436807591 \h 32 Sampling event  PAGEREF _Toc436807592 \h 32 Appendix B - Alaska Cruise Ship Data Review Checklist  PAGEREF _Toc436807593 \h 34 Appendix C QAPP Deadlines*  PAGEREF _Toc436807594 \h 35 Applications:  PAGEREF _Toc436807595 \h 35 Notifications:  PAGEREF _Toc436807596 \h 35 Analytical Reports:  PAGEREF _Toc436807597 \h 35 Audits  PAGEREF _Toc436807598 \h 35 Reporting:  PAGEREF _Toc436807599 \h 35 Appendix D VSSP Blank Copy  PAGEREF _Toc436807600 \h 36  A.3 DISTRIBUTION LIST This list includes the names and addresses of those who receive copies of the approved QAPP and subsequent revisions. Table 1 Distribution ListNAMEPOSITIONAGENCY/ CompanyDIVISION/ BRANCH/SECTIONCONTACT INFORMATION Project ManagerCruise ship OperatorPhone: Email:Sampling & Analysis ManagerCruise ship OperatorOperator or under contract to Cruise ship OperatorPhone: Email:Albert FaureProject ManagerADECDivision of Water/CPVECPhone: 907-465-5279 Email: HYPERLINK "mailto:albert.faure@alaska.gov" albert.faure@alaska.govDouglas KolwaiteQA OfficerADECDivision of Water/ WQSAR/QA907-465-5305 Email: HYPERLINK "mailto:douglas.kolwaite@alaska.gov" douglas.kolwaite@alaska.gov A.4 PROJECT TASK/ORGANIZATION Duties and responsibilities of key individuals are listed in Table 2, Position Organizational Responsibilities: Table 2 Position Organizational ResponsibilitiesAgency/CompanyPosition TitleResponsibilitiesADEC DOW Cruise ship ProgramProject ManagerThe ADEC Project Manager is responsible for managing the program to meet the requirements in the Alaska Statute, regulation and the approved QA/QC plan. ADEC DOWQA OfficerResponsible for QA review and approval of plan and oversight of QA activities ensuring collected data meets projects stated data quality goals.ADEC DOWEnv. Program SpecialistReview sample data. Cruise ship OperatorProject ManagerThe project manager is responsible for compliance with this Generic Small Cruise ship Quality Assurance Project Plan (QAPP). Responsibilities include: Ensuring coordination among vessels crew, samplers, lab, and ADEC. Communicating project information to the sampler, lab, and ADEC Assuring that project participants have necessary training. Fielding questions and requests for information that arises during and after the project. Managing the financial aspect of the project. Attaching field notes to sample results, chain of custody and providing the ADEC with any deviations to the QAPP or VSSP.Cruise ship Operator or entity under contract to the Cruise ship OperatorSampling Manager/TeamThe sampling manager responsibilities include: Will design a tentative sampling schedule. Will submit the schedule to the ADEC with the vessel specific sampling plan (VSSP). Will ensure coordination among vessels crew, samplers, lab, and ADEC for all monitoring operations. Will notify the ADEC a minimum of 36 hours prior to the sampling. This notice gives ADEC the opportunity to audit the ships sampling procedures. Will be responsible for sample collection, sample integrity and custody, field measurements, and accurate notes. Will provide to the laboratory personnel and vessel representative and the Project Quality Assurance Officer upon completion of all sampling a compilation of field notes, deviations from VSSP or QA/QCP plans (if applicable), and Chain of Custody.Laboratory under contract to the Cruise ship OperatorLaboratory ManagerResponsible for the timely analysis of samples and overall review and approval of contracted laboratory analytical work, responding to sample result inquiries and method specific details.Laboratory QA Manager/OfficerResponsible for QA/QC of water quality laboratory analyses as specified in the QAPP. Along with Laboratory Manager, the Lab QA Officer reviews and verifies the validity of sample data results as specified in the QAPP and appropriate EPA approved analytical methods  SHAPE \* MERGEFORMAT  A.5 PROBLEM DEFINITION/BACKGROUND AND PROJECT OBJECTIVES A.5.1 Problem Definition This document is prepared and submitted to fulfill requirements of Alaska Statute 46.03.460- 46.03.490, and 18 AAC 69.025. ADEC requires at least two sampling events per vessel in a season. A sampling event is the collection of representative samplesof each wastewater type being discharged within Alaska waters. The number of samples in a sampling event is based on the ship configuration, vessel wastewater management practices, and the wastewater quantities discharged while the sample team is on-board. The samples must be taken at a point in the system directly before being discharged overboard as determined by the approved VSSP. The samples must be taken while the vessel is discharging into ambient water. A.5.2 Project Background Alaska law requires that the owner or operator of a small commercial passenger vessel (50 to 249 overnight passengers), registered under the Commercial Passenger Vessel Environmental Compliance (CPVEC) Program, may not discharge treated sewage, graywater and other wastewater in Alaska waters unless the vessel meets certain requirements, such as sampling. The original law was enacted in 2001. In 2004 it was modified to allow alternative terms and conditions to discharge- this established Best Management Practices Plans to allow discharge that exceeded Alaska Water Quality Standards if ADEC accepts a plan to minimize environmental impact of those vessels. These alternative terms and conditions are initially set to expire by 2016. However, in 2013 statutory changes re-instated the alternative terms and conditions, and removed the expiration date. ADEC has been collecting wastewater data from small vessels which discharge for nearly 10 years. A.5.3Project Objective(s) The objectives of this document are to guide the collection of quality and representative wastewater samples from small cruise ships in Alaskan waters to ensure compliance with ADEC water quality standards under the Cruise ship Program. A.6 PROJECT/TASK DESCRIPTION and SCHEDULE A.6.1Project Description This QAPP specifies the minimum requirements for sampling and analysis of treated sewage and/or graywater and other wastewaters as defined in AS 46.03.490 for vessels discharging into the waters of the Alexander Archipelago as defined in AS 46.03.490. All sampling events required by AS 46.03 shall be conducted in accordance with this QAPP. Owner/operator must provide documentation verifying their compliance with the guidelines in AS 46.03.460-46.03.490, and 18 AAC 69, 18 AAC 70 and this plan. Each participating ship must be sampled within 45 days of initial entry into Alaska waters and be subject to DEC sampling audits. The ADEC may perform additional sampling and analysis inspections as necessary to implement AS 46.03. This QAPP covers sampling and analysis for the parameters listed below. Analysis for conventional pollutant parameters required by ADEC under AS 46.403.465 and is noted by an asterisk (*). ADEC will also require analysis of priority pollutant parameters under AS 46.403.465. A sample that fails to provide valid results for all required pollutants as indicated by an asterisk will not be counted as an acceptable sample for purposes of meeting the minimum requirement of two samplings for conventional pollutants. Conventional pollutants: Total Suspended Solids (TSS)*Settleable Solids (SS)Biochemical Oxygen Demand (BOD)*Oil and Grease Chemical Oxygen Demand (COD)* Total Organic CarbonAmmonia Total*Specific Conductance Fecal Coliform*AlkalinitypH*Total Nitrogen (Ammonia, Nitrate, Nitrite, and Total Kjeldahl Nitrogen (TKN))Total and Free Residual Chlorine*Total Phosphorus The Sampling of the vessel waste water discharge is defined in the Vessel Specific Sampling Plan (VSSP). The sampling of the pollutants of interest, type of sampling, the sample waste stream etc. are listed in the approved VSSP. Hardness Base/Neutrals, Acids Volatile Organic Chemicals (VOCs) Trace Metals (Total Recoverable and Dissolved) Samples may be analyzed in the field or only by a laboratory with current certification under one of the following laboratory certification programs: ADEC-Drinking Water Certified Laboratory for Chemistries ADEC-Drinking Water Certified Laboratory for Microbiological / Fecal Coliforms. Washington State Department of Ecology (WA DOE) Certified Water/Wastewater Laboratory for Chemistries. NELAC Certified Water/Wastewater Laboratory Note 1: ADEC certifies laboratories for drinking water and contaminated sites analysis only. At the present time, ADEC does not certify laboratories for water/wastewater analyses. However, even though water/wastewater methodologies may differ somewhat from drinking water analytical methods, an ADEC drinking water-approved laboratory lends credibility to a laboratorys quality assurance and quality control processes. A list of ADEC-approved microbiological laboratories is available at:  HYPERLINK http://www..state.ak.us/dec/deh/water/labs.htm http://www..state.ak.us/dec/deh/water/labs.htm and for laboratories providing chemical analysis at:  HYPERLINK http://www.state.ak.us/dec/deh/water/chemlabs.htm http://www.state.ak.us/dec/deh/water/chemlabs.htm. Note 2: For microbiological analyses, only a laboratory with current ADEC drinking water certification that resides within Alaska may be used. Due to the short sample holding time requirements (< 6 hours) labs outside of Alaska would not reasonably be able to receive and start the analysis as specified by the EPA water/wastewater approved microbiological methods. Note 2: NELAC and/or WA DOE laboratory certification for water/wastewater chemistries is preferred since certification is specific to the pollutants and matrices of interest. A.7 DATA QUALITY OBJECTIES AND CRITERIA FOR MEASUREMENT DATA A.7.1 Data Quality Objectives (DQOs) Data Quality Objectives (DQOs, EPAQA/G4). DQOs are qualitative and quantitative statements derived from the DQO Process that: Clarify the monitoring objectives (i.e., determine water/wastewater pollutant concentrations of interest and how these values compare to water quality standards regulatory limits Define the appropriate type of data needed. In order to accomplish the monitoring objectives, the appropriate type of data needed is defined by the respective WQS. For WQS pollutants, compliance with the WQS is determined by specific measurement requirements. The measurement system is designed to produce water pollutant concentration data that are of the appropriate quantity and quality to assess compliance. A.7.2 Measurement Quality Objectives (MQOs) Measurement Quality Objectives (MQOs) are a subset of DQOs. MQOs are derived from the monitoring projects DQOs. MQOs are designed to evaluate and control various phases (sampling, preparation, and analysis) of the measurement process to ensure that total measurement uncertainty is within the range prescribed by the projects DQOs. MQOs define the acceptable quality (data validity) of field and laboratory data for the project. MQOs are defined in terms of the following data quality indicators: Detectability Precision Bias/Accuracy Completeness Representativeness Comparability Detectability is the ability of the method to reliably measure a pollutant concentration above background. DEC DOW uses two components to define detectability: method detection limit (MDL) and practical quantification limit (PQL) or reporting limit (RL). Individual analyte MDL and PQL limits are listed in Table 4. The MDL is the minimum value which the instrument can discern above background but no certainty to the accuracy of the measured value. For field measurements the manufacturers listed instrument detection limit (IDL) can be used. The PQL or RL is the minimum value that can be reported with confidence (usually some multiple of the MDL). Sample data measured below the MDL is reported as ND or non-detect. Sample data measured e" MDL but d" PQL or RL is reported as estimated data. Sample data measured above the PQL or RL is reported as reliable data unless otherwise qualified per the specific sample analysis. Precision is the degree of agreement among repeated measurements of the same parameter and provides information about the consistency of methods. Precision is expressed in terms of the relative percent difference between two measurements (A and B). For field measurements, precision is assessed by measuring replicate (paired) samples at the same locations and as soon as possible to limit temporal variance in sample results. Field and laboratory precision is measured by collecting blind (to the laboratory) field replicate or duplicate samples. For paired and small data sets project precision is calculated using the following formula:  EMBED Equation.3  Bias (Accuracy) is a measure of confidence that describes how close a measurement is to its true value. Acceptance limits for Bias for each analyte are listed in Table 2. Methods to determine and assess accuracy of field and laboratory measurements include, instrument calibrations, various types of QC checks (e.g., sample split measurements, sample spike recoveries, matrix spike duplicates, continuing calibration verification checks, internal standards, sample blank measurements (field and lab blanks), external standards), performance audit samples (DMRQA, blind Water Supply or Water Pollution PE samples from A2LA certified, etc. Bias/Accuracy is usually assessed using the following formula:  EMBED Equation.3  Completeness is a measure of the percentage of valid samples collected and analyzed to yield sufficient information to make informed decisions with statistical confidence. The completeness criterion for this project is 80 percent of the compiled analytical data per each analytical parameter for each vessel participating in the program. Because of the variety of vessels and discharges sampled, and the possibility for weather or other shipping-related delays resulting in missed holding times, a completeness criterion of less than 100% is to be expected. Project completeness is determined for each pollutant parameter using the following formula: T (I+NC) x (100%) = Completeness T Where T = Total number of expected sample measurements. I = Number of invalid sample measured results. NC = Number of sample measurements not produced (e.g. spilled sample, etc). Representativeness Representativeness is a measure of how well the sample reflects the typical wastewater effluent. Sample representativeness will be established by collecting cruise ship graywater, blackwater, and other wastewater discharge samples following vessel specific sampling plans (VSSP). The owner and operator is responsible for developing and submitting VSSPs to both agencies for each vessel participating in the program The treatment system effluent will be considered representative for the two samples only if the vessel normally discharges continuously. The VSSP is designed to ensure that consistent sampling methods are followed and that samples are collected from appropriate and representative locations at appropriate times. Vessel operation that differs from the VSSP may result in State of Alaska rejection of samples. Comparability is a measure that shows how data can be compared to other data collected by using standardized methods of sampling and analysis. Comparability is shown by referencing the appropriate measurement method approved by as specified in federal and/or state regulatory and guidance documents/methods for the parameter/s to be sampled and measured (e.g., ASTM, Standard Methods, Alaska Water Quality Standards ( HYPERLINK "http://www.dec.state.ak.us/water/wqsar/wqs/index.htm" http://www.dec.state.ak.us/water/wqsar/wqs/index.htm, EPA Guidelines Establishing Test Procedures for the Analysis of Pollutants Under the Clean Water Act; National Primary Drinking Water Regulations; and National Secondary Drinking Water Regulations; Analysis and Sampling Procedures  HYPERLINK "http://www.epa.gov/fedrgstr/EPA-WATER/2007/March/Day-12/w1073.htm" http://www.epa.gov/fedrgstr/EPA-WATER/2007/March/Day-12/w1073.htm etc)). As with representativeness and completeness, comparability is determined during project development and must be specified in the QAPP. For each parameter to be sampled/measured, list the measurement method to be used and the MQOs to meet the overall data quality objectives. This applies to both direct field measurements (e.g., field pH meters, DO meters, etc.) as well as samples collected for subsequent laboratory analyses. Because of the different source types found on different vessels (e.g., a holding tank on some ships may contain both blackwater and graywater, while on others it may only contain graywater), careful definition of discharge types will be made in the VSSP. It is essential that these definitions be carried through to the end data user, as these differences could erroneously bias data interpretation. The sampling team must make full use of ship records and logs, especially the Graywater and Sewage Discharge Record Book which includes the latitude and longitude at the beginning and end of discharge, identifying tanks, estimating volumes and calculating discharge rates (if any) at the time the sample is drawn. If the vessel is discharging continuously (not just certified but actually is in practice) then the sampler does not need to record latitude and longitude at the beginning and end of discharge, identifying tanks, estimating volumes of those tanks. The sampler needs to identify which treatment unit is discharging and the discharge rate. The vessel speed and longitude/latitude must be obtained by the sampler if the sample is taken while the vessel is discharging underway. Information added to the VSSP or changes to the VSSP during the sampling event must be recorded on the VSSP, COC, or in the field notes and must accompany the samples to the lab and be provided to the project data recipients as part of the complete unannounced sampling report. Table 4. Project Measurement Quality Objectives (MQOs)GroupAnalyteMethod MDL (mg/L)PQL (mg/L)Alaska WQSPrecision (RSD)Accuracy (% Rec)Aquatic Life Marine Water AcuteChronicConventional PollutantsFecal ColiformsSM 9222 D2 fcu/100ml2fcu/100mlUSGS 200fcu/100mlAlkalinitySM 2320 B-1997520<2085-115Ammonia - TotalEPA 350.1 or Hach 102050.150.5<2080-120Biochemical Oxygen Demand (BOD)EPA 405.1 or SM 521022<2070-130Chemical Oxygen Demand (COD)EPA 410.4 Rev 2.0515<2085-115Chlorine Residual (total/free)SM 4500-Cl G0.10.1Oil and GreaseEPA 1664 B1.55<2060-150pHSM 4500, EPA 150.10.1 std units0.1 std units<20Settleable SolidsSM 2540 F0.1 ml/L0.1ml/L<20Specific ConductanceSM 2510 B-19971 mhos/cm2 mhos/cm<2085-115Total NitrogenEPA 351.2 Rev 2.015Total Organic CarbonSM 5310 C0.31<2085-115Total PhosphorusEPA 365.1 Rev 2.00.030.1<2085-115Total Suspended Solids (TSS)EPA 160.2 SM 2540 D1.34USGS 150 mg/L<2085-115Priority PollutantsAnalyteMethodMDL (g/L)PQL (g/L)Alaska WQS Precision (RSD)Accuracy (% Rec)Aquatic Life Marine WatersAcuteChronicTotal Aromatics and Total Aqueous Hydrocarbons using VOC and BNA dataVOCs1,1,1,2-TetrachloroethaneEPA 624 Rev 7/950.52<2075-1251,1,1-TrichloroethaneEPA 624 Rev 7/951.55<2052-1621,1,2,2-TetrachloroethaneEPA 624 Rev 7/951.55<2046-1571,1,2-TrichloroethaneEPA 624 Rev 7/951.55<2052-1501,1-DichloroethaneEPA 624 Rev 7/951.55<2059-1551,1,-DichloroetheneEPA 624 Rev 7/951.55<205-2341,1-DichloropropeneEPA 624 Rev 7/951.55<2075-1251,2,3-TrichlorobenzeneEPA 624 Rev 7/951.55<2075-1251,2,3-TrichloropropaneEPA 624 Rev 7/951.55<2080-1201,2,4-TrichlorobenzeneEPA 624 Rev 7/951.55<2075-1251,2,4-TrimethylbenzeneEPA 624 Rev 7/951.55<2075-1251,2-Dibromo-3-ChloropropaneEPA 624 Rev 7/95310<2070-1301,2-DichlorobenzeneEPA 624 Rev 7/95310<2018-1901,2-DichloroethaneEPA 624 Rev 7/951.55<2049-1551,2-DichloropropaneEPA 624 Rev 7/951.55<205-2101,3,5-TrimethylbenzeneEPA 624 Rev 7/950.52<2070-1301,3-DichlorobenzeneEPA 624 Rev 7/95310<2059-1561,3-DichloropropaneEPA 624 Rev 7/950.52<2075-1301,4-DichlorobenzeneEPA 624 Rev 7/95310<2018-1902,2-DichloropropaneEPA 624 Rev 7/951.55<2060-1302-ButanoneEPA 624 Rev 7/951550<2060-1402-Chloroethyl Vinyl EtherEPA 624 Rev 7/95310<2010-3052-ChlorotolueneEPA 624 Rev 7/95310<2075-1352-HexanoneEPA 624 Rev 7/95620<2060-140EPA 624 Rev 7/954-ChlorotolueneEPA 624 Rev 7/95310<2075-1304-IsopropyltolueneEPA 624 Rev 7/9513<2075-1254-Methyl-2-PentanoneEPA 624 Rev 7/95620<2060-140AcetoneEPA 624 Rev 7/951050<2040-60AcroleinEPA 624 Rev 7/9530100<2040-160AcrylonitrileEPA 624 Rev 7/9530100<2065-130BenzeneEPA 624 Rev 7/951.55<2037-151BromobenzeneEPA 624 Rev 7/951.55<2075-130BromochloromethaneEPA 624 Rev 7/9513<2035-155BromodichloromethaneEPA 624 Rev 7/951.55<2080-130BromoformEPA 624 Rev 7/951.55<2045-169BromomethaneEPA 624 Rev 7/95310<2010-242Carbon DisulfideEPA 624 Rev 7/95310<2060-130Carbon TetrachlorideEPA 624 Rev 7/951.55<2070-140ChlorobenzeneEPA 624 Rev 7/951.55<2037-160ChloroethaneEPA 624 Rev 7/95310<2014-230ChloroformEPA 624 Rev 7/951.55<2051-138ChloromethaneEPA 624 Rev 7/95310<2010-273Cis-1,2-DichloroetheneEPA 624 Rev 7/951.55<2080-130Cis-1,3-DichloropropeneEPA 624 Rev 7/951.55<205-227DibromochloromethaneEPA 624 Rev 7/951.55<2053-149DibromomethaneEPA 624 Rev 7/951.55<2080-130DichlorodifluoromethaneEPA 624 Rev 7/95310<2060-140EthylbenzeneEPA 624 Rev 7/951.55<2037-162HexachlorobutadieneEPA 624 Rev 7/951550<2050-130IodomethaneEPA 624 Rev 7/951.55<2050-150IsopropylbenzeneEPA 624 Rev 7/951.5<2070-130m&p XylenesEPA 624 Rev 7/951.55<2075-120Methylene ChlorideEPA 624 Rev 7/95310<2010-221n-ButylbenzeneEPA 624 Rev 7/951.55<2070-130n-PropylbenzeneEPA 624 Rev 7/95310<2070-130o-XyleneEPA 624 Rev 7/951.55<2080-125sec-ButylbenzeneEPA 624 Rev 7/951.55<2070-130StyreneEPA 624 Rev 7/951.55<2085-125Tert-Butyl Methyl EtherEPA 624 Rev 7/951.55<2070-130Tert-ButylbenzeneEPA 624 Rev 7/951.55<2070-125TetrachloroetheneEPA 624 Rev 7/951.55<2064-148TolueneEPA 624 Rev 7/951.55<2047-150trans-1,2-DichloroetheneEPA 624 Rev 7/951.55<2054-156trans-1,3-DichloropropeneEPA 624 Rev 7/951.55<2017-183Trans-1,4-Dichloro-2-ButeneEPA 624 Rev 7/95310<2070-130TrichloroetheneEPA 624 Rev 7/951.55<2071-157TrichlorofluoromethaneEPA 624 Rev 7/95310<2017-181TrichlorotrifluoroethaneEPA 624 Rev 7/95310<2060-140Vinyl AcetateEPA 624 Rev 7/951.55<2060-140Vinyl ChlorideEPA 624 Rev 7/950.52<202-251BNA 1,2-DiphenylhydrazineEPA 625 Rev 7/951.55<4060-1402,4,5-TrichlorophenolEPA 624 Rev 7/951.55<4060-1402,4,6-TrichlorophenolEPA 624 Rev 7/951.55<4037-1442,4-DichlorophenolEPA 624 Rev 7/951.55<4055-1302,4-DimethylphenolEPA 624 Rev 7/95515<4015-1302,4-DinitrophenolEPA 624 Rev 7/95825<4025-1912,4-DinitrotolueneEPA 624 Rev 7/951.55<4039-1392,6-DinitrotolueneEPA 624 Rev 7/951.55<4050-1582-ChloronapthaleneEPA 624 Rev 7/95210<4030-1702-ChlorophenolEPA 624 Rev 7/951.55<4023-1342-MethylnapthaleneEPA 624 Rev 7/951.55<4040-1402-MethylphenolEPA 624 Rev 7/951.55<4050-1152-NitroanalineEPA 624 Rev 7/951.55<4050-1152-NitrophenolEPA 624 Rev 7/951.55<4050-1153&4MethylphenolEPA 624 Rev 7/951.55<4030-1253,3-DicholorbenzidineEPA 624 Rev 7/95525<4030-1703-NitroanilineEPA 624 Rev 7/951550<4030-1704,6-Dinitro-2-methylphenolEPA 624 Rev 7/95825<4025-1814-Bromophenyl Phenyl etherEPA 624 Rev 7/951.55<4050-1404-Chloro-3-methylphenolEPA 624 Rev 7/95310<4022-1474-ChloroanilineEPA 624 Rev 7/951.55<4030-1704-Chlorophenyl methyl sulfoneEPA 624 Rev 7/95620<4030-1704-Chlorophenyl Phenyl etherEPA 624 Rev 7/951.55<4050-1504-NitroanilineEPA 624 Rev 7/951550<4040-1104-NitrophenolEPA 624 Rev 7/95825<4025-132AcenaphtheneEPA 624 Rev 7/951.55<4040-145AcennaphthyleneEPA 624 Rev 7/951.55<4033-145AnthraceneEPA 624 Rev 7/951.55<4027-133BenzidineEPA 624 Rev 7/9550200<4030-170Benzo (A) AnthraceneEPA 624 Rev 7/951.55<4033-143Benzo (A) PyreneEPA 624 Rev 7/951.55<4017-163Benzo (B) FluorantheneEPA 624 Rev 7/951.55<4024-159Benzo (g,h,i) PeryleneEPA 624 Rev 7/951.55<405-219Benzo (K) FluorantheneEPA 624 Rev 7/951.55<4011-162Benzoic AcidEPA 624 Rev 7/951.55<405-110Benzyl AlcoholEPA 624 Rev 7/95310<4024-149Bis (2-Chloroethoxy) methaneEPA 624 Rev 7/951.55<4033-184Bis (2-chloroethyl) etherEPA 624 Rev 7/951.55<4025-158Bis (2-Chloroisopropyl) etherEPA 624 Rev 7/951.55<4036-166Bis (2-Ethylhexyl) PhthalateEPA 624 Rev 7/951.55<4085-158Butyl Benzyl PhthalateEPA 624 Rev 7/951.55<405-152ChryseneEPA 624 Rev 7/951.55<4017-168Dibenzo (a,h) AnthraceneEPA 624 Rev 7/951.55<405-227DibenzofuranEPA 624 Rev 7/951.55<4050-130Diethyl PhthalateEPA 624 Rev 7/951.55<405-114Dimethyl PhthalateEPA 624 Rev 7/951.55<405-112Di-N-Butyl PhthalateEPA 624 Rev 7/951.55<4060-160Di-N-Octyl PhthalateEPA 624 Rev 7/951.55<405-146FluorantheneEPA 624 Rev 7/951.55<4026-137FluoreneEPA 624 Rev 7/951.55<4055-130HexachlorobenzeneEPA 624 Rev 7/951.55<405-152HexachlorocyclopentadieneEPA 624 Rev 7/95310<4030-170HexachloroethaneEPA 624 Rev 7/951.55<4040-140Indeno (1,2,3-CD) PyreneEPA 624 Rev 7/951.55<405-171IsophoroneEPA 624 Rev 7/951.55<4021-196NapthaleneEPA 624 Rev 7/95310<4021-133NitrobenzeneEPA 624 Rev 7/951.55<4035-180N-NitrosodimethylamineEPA 624 Rev 7/951.55<4030-170N-Nitrosodi-N-PropylamineEPA 624 Rev 7/951.55<405-123N-NitrosodiphenylamineEPA 624 Rev 7/95310<4060-140PentachlorophenolEPA 624 Rev 7/95825<4025-176PhenanthreneEPA 624 Rev 7/951.55<4050-140PhenolEPA 624 Rev 7/951.55<405-112PyreneEPA 624 Rev 7/951.55<4045-135MetalsTotal Recoverable MetalsAntimonyEPA 200.8 Rev 5.40.82.52093-107ArsenicEPA 200.8 Rev 5.40.82.52080-120BerylliumEPA 200.8 Rev 5.40.51.52088-112CadmiumEPA 200.8 Rev 5.40.652.02094-108ChromiumEPA 200.8 Rev 5.40.82.52091-114CopperEPA 200.8 Rev 5.40.41.02094-107LeadEPA 200.8 Rev 5.40.31.02094-114Mercury (Total)EPA 200.8 Rev 5.40.652.02080-120NickelEPA 200.8 Rev 5.40.51.52085-115SeleniumEPA 200.8 Rev 5.41.55.002080-120SilverEPA 200.8 Rev 5.40.31.02085-115ThalliumEPA 200.8 Rev 5.40.31.02085-115ZincEPA 200.8 Rev 5.40.92.52080-120Dissolved MetalsAntimonyEPA 200.8 Rev 5.40.82.5<2085-115ArsenicEPA 200.8 Rev 5.40.842.5<2085-115BerylliumEPA 200.8 Rev 5.40.51.5<2085-115CadmiumEPA 200.8 Rev 5.40.652.0<2085-115ChromiumEPA 200.8 Rev 5.40.82.5<2085-115CopperEPA 200.8 Rev 5.40.441.0<2085-115LeadEPA 200.8 Rev 5.40.31.0<2085-115NickelEPA 200.8 Rev 5.40.51.5<2085-115SeleniumEPA 200.8 Rev 5.41.55<2085-115SilverEPA 200.8 Rev 5.40.31.0<2085-115ThalliumEPA 200.8 Rev 5.40.31.0<2085-115ZincEPA 200.8 Rev 5.40.92.5<2085-115 A.8 SPECIAL TRAINING REQUIREMENTS/CERTIFICATION Samplers will be trained in sampling methods, sample handling, chain of custody, and field measurements as outlined in 40 CFR 136. Additionally, samplers will receive appropriate training through their employer or their employers designee, in any necessary shipboard safety procedures. Laboratories used will have a current Alaska Department of Environmental Conservation Drinking Water certification or be a current NELAC certified laboratory. Due to the short holding time for fecal coliform samples collected within Alaska, only DEC Drinking Water Certified laboratories will be used. Laboratory analysts will be trained in accordance with each laboratorys QA Plan and Standard Operating Procedures (SOPs). A.9 DOCUMENTS AND RECORDS A.9.1 Sample schedule and Vessel/Sample Identification The sampler must include a tentative schedule in the Vessel Specific Sampling Plan. The sampler must also notify the ADEC of its intent to sample at least 36 hours prior to sample collection. The two sampling events must be a minimum of 21 days apart unless being conducted as a re-sampling allowed under 18 AAC 69.070. Samples will be identified clearly on the chain of custody and sample bottles. For example, a sample from the Graywater from the M/V Hypothetica will be identified as Graywater Overboard Discharge, as the description with associated dates and times. The Sample ID should clearly state where the sample was taken. For example, a mixed black and gray sample taken from the MSD discharge line is MSD BW as its sample ID. Holding tanks should be HT. Collection tanks should be labeled CT. All samplers should use the same sample ID system A.9.2 Field Records (Required for ADEC compliance samples) Field notes will be collected in bound field notebooks with numbered pages or recorded on pre-printed forms with specific information pertaining to the sampling event. On-board staff will witness the sampling and will initial the field notes. Included in the field notes for each sample are: Vessel name (e.g., M/V Hypothetica), Sampling personnel, Shipboard assistants, Signature or initials by the vessel crew in the field notes indicating that the sample port is correct, Sample date and times, Field measurements: pH, free chlorine, total chlorine, and temperature, Records on discharge flow rates (always) and holding tank volumes (only for underway sampling), Samples collected, Nature of sample: Composite or Grab, Waste type: blackwater, graywater, or mixed, Deviations from VSSP and/or QAPP, Unusual conditions and explanation of data anomalies, Latitude/longitude and speed at time of discharge being sampled (only for underway sampling), Copy of the Discharge record for the sampled discharge, which will include records on discharge flow rates (always) and holding tank volumes (only for underway sampling).  A.9.3 Laboratory Records Upon completion of laboratory analysis, laboratory data review, and data validation, the laboratory will issue a full report in a level III electronic format describing the results of analysis for each sample submitted. A.9.4 Chain of Custody The original chain of custody form will accompany the sample to the laboratory. When portions of the sample are sent to another laboratory (e.g., for many of the priority pollutants), a copy of the chain of custody will be made and this will accompany the samples. At each transfer of the sample, the transfer will be indicated on the chain of custody form. The person listed on the Chain of Custody should have full sight or control of the sample at all times until it the COC is relinquished by that person and received by the next party signed on the COC. A copy of the original chain of custody will be included with the final report, including copies of the COCs transferring samples to other labs. Table 6. Project Documents and RecordsCategoriesRecord/Document TypesLocationRetention TimeVessel Specific Sampling PlanAnnual approved VSSPVessel, Project ManagerUntil replaced by updated VSSPEnvironmental Data OperationsQA Project PlanADEC/Vessel5 yearsField NotebooksVessel5 yearsSample collection/measurement recordsVessel5 yearsSample Handling & Custody RecordsVessel5 yearsChemical labels, MSDS sheetsVessel5 yearsInspection/Maintenance RecordsVessel5 yearsRaw DataLab data (sample, QC and calibration) including data entry formsLab5 years In addition to any written report, data collected for a project will be submitted electronically to ADEC via a CD ROM, ZIP Disk or email file. All dates are to be formatted as MM-DD-YYYY. B. DATA GENERATION AND ACQUISITION B.1 SAMPLING PROCESS DESIGN A vessel specific sampling plan (VSSP) will be developed for each ship by the project manager and submitted to the sampling team 21 days prior to sampling. This plan needs to be approved by the ADEC. The plan will include elements listed in 18 AAC 69.030, and as a minimum, the following: Vessel name. Passenger and crew capacity of ship. Daily water use per individual. Locations and capacities for treated sewage, graywater, and other wastewater tanks. Type of wastewater treatment systems. Each discharge pump type and rate Vessel schematic of discharge ports and corresponding sampling ports. Description of discharges, including anticipated flow rates and tank volumes. Table containing type of discharge, type of sample (grab or composite), parameters (conventional or priority pollutants), location on the vessel where each sample is to be collected, and special circumstances. A narrative description of the time at which each sample is to be taken based upon circumstances that will yield a sample most likely to be representative of the average discharge that passes through the location where the sample is taken A description of the standards the owner or operator will use to determine a deviation from the plan Equipment required. Each VSSP will be dated and a copy will be provided to the ADEC. The ADEC must approve the VSSP prior to sampling. After the first sampling event on a vessel, the VSSP may be updated. If it is updated, copies of the updated sampling plan and approved by the ADEC before the second round of sampling occurs. B.2 SAMPLING METHOD REQUIREMENTS Specific sampling techniques for each vessel will be detailed in the VSSP. The following general guidelines are listed to provide consistency among the vessels utilizing this QAPP. Samples will reflect a representative discharge of treated blackwater, graywater and other wastewaters into applicable waters of Alaska from an operable marine sanitation device, other treatment system, a holding tank or some combination as specified in the VSSP. In port sampling, in compliance with ADEC sampling events, will be conducted only if the vessel is certified to discharge in port. If samples must be taken while the ship is underway, care will be taken to assure sample representativeness and homogeneity. See VSSP for further details on sampling. Samplers will work in teams of two for sampling events that must be performed while the vessel is underway to ensure that proper sampling techniques are followed, adequate notes are taken during the sampling event, and proper sample custody is maintained. One sampler will be sufficient for all in-port sampling events. Samplers should wear disposable gloves and safety eyewear, if needed, and observe precautions while collecting samples, remaining aware of the potential chemical and biological hazards present. The Project Sampling Staff collecting samples will take care not to touch the insides of bottles or lids/caps during sampling. Samplers will contain all solid and liquid wastes generated during sampling (used gloves, paper towels, chlorine test waste, and overflow from filling of VOC sampling vials) and will dispose of it properly at the conclusion of the sampling event. Samplers will take care not to touch the insides of bottles or lids/caps during sampling. B.2.1 Sample Types Samples will be listed as composite or grab on the Chain-of- Custody or Transmission Form and in field logbook or field data sheets. B.2.2 Sample Containers and Equipment In this section describe specific sample handling and custody requirements (If the results of a sampling program may be used as evidence, a strict written record (Chain of Custody) must be documented tracking location and possession of the sample/data at all times). All sampling equipment and sample containers will be cleaned according to the equipment specifications and/or the analytical laboratory. Bottles supplied by a laboratory are pre-cleaned and must never be rinsed, and will be filled only once with sample. For samples requiring cooling, a temperature blank shall accompany each cooler (min/max thermometer preferred). The thermometer shall be certified NIST traceable, readable to at least 0.2C and within the listed certification period (Note: Infrared thermometers are unacceptable for use in measuring temperature blanks and sample shipment/receipt temperatures). Use example table below to list specific analyte/method criteria for parameter holding times and preservation methods. TABLE 8 Sample Containers, Preservations, Holding Times, and Sample Types PARAMETERCONTAINERPRESERVATIONMAXIMUM HOLDING TIMEGrab or CompositeMinimum Representative VolumeConventional PollutantsTotal Suspended SolidsP, FP, GCool, d"6 C7 daysGrab Only100 mlSettleable SolidsP, FP, GCool, d"6 C48 hoursGrab Only1000 mlBiochemical Oxygen DemandP, FP, GCool, d"6 C48 hoursGrab Only1000 mlAmmonia  TotalP, FP, GCool, d"6 C, H2SO4 to pH <228 daysGrab Only400 mlChemical Oxygen DemandP, FP, GCool, d"6 C, H2SO4 to pH <228 daysGrab Only50 mlSpecific ConductanceP, FP, GCool, d"6 C28 daysGrab Only100 mlFecal ColiformsSterile PA, GCool, d"10 C with no indication of sample freezing 0.0008% Na2S2O3 6 hours sample collection to lab receipt. 2 hours lab sample receipt to incubation start. Note: hold times not additiveGrab Only100 mlAlkalinityP, FP, GCool, d"6 C14 daysGrab Only100 mlpH P, FP, GNone requiredAnalyze within 15 minutes in field Grab Only25 mlOil and GreaseGCool, d"6 C, HCL or H2SO4 to pH <228 daysGrab Only1000 mlTotal Organic CarbonP, FP, GCool, d"6 C, HCL, H2SO4 or H3PO4 to pH <228 daysGrab Only50 mlTotal Kjeldahl NitrogenP, FP, GCool, d"6 C, H2SO4 to pH <228 daysGrab Only500 mlTotal PhosphorusP, FP, GCool, d"6 C, H2SO4 to pH <228 daysGrab Only50 mlTemperatureP, FP, GNone requiredAnalyze ASAP in fieldGrab Only1000 mlChlorine ResidualP, GNone requiredAnalyze within 15 minutes in fieldGrab Only100 mlPriority PollutantsBNAG, FP-lined cap Cool, d"6 C, 0.008% Na2S2O3 if residual chlorine is detected above 0.1 mg/L7 days until extraction, 40 days after extractionGrab Only1000 ml VOCsG, FP-lined septumCool, d"6 C, 0.008% Na2S2O3 if residual chlorine is detected above 0.1 mg/L ,HCL to pH <2 14 days Grab Only40 mlTotal Aromatic and Total Aqueous HydrocarbonsSee BNAs and VOCsTotal Mercury (CVAA) P, FP, GHNO3 to pH <228 daysGrab Only100 mlTotal Recoverable Metals  P, FP, G HNO3 to pH <2, or at least 24 hours prior to analysis6 monthsGrab Only100 mlDissolved Metals P, FP, GFiltration w/0.45 micron filter within 15 minutes of sample collection,, HNO3 to pH <26 monthsGrab Only200 ml B.2.3 Sampling Methods The required field tests will be performed prior to sampling in order to determine if residual chlorine is present. This will dictate the preservation procedures for the VOC and BNA analyses. The practical quantitation limit for chlorine testing using field equipment is 0.1 mg/L. Some field instruments may display values below this level. Any values observed below this limit will be recorded as actual readings on the field notes but as <0.1 mg/L final data reports. Sample containers will normally be pre-preserved by the laboratory. If chlorine residual is detected above 0.1 mg/L during field measurement of chlorine, ascorbic acid provided by the lab will be added in the field to the BNA until no chlorine is detected. The lab must provide decanting bottles with ascorbic acid. When chlorine is detected, the sample will be added first to the decanting bottle, and then will be decanted into the VOC vials. Sample fractions for microbiology will be cooled immediately in an ice-water bath and then placed into a cooler containing frozen blue ice or ice and water mixture to maintain a sample temperature of 0 - 10 C. Temperature will be measured and recorded at the time of sample collection and a note shall be made of the temperature of the cooler contents upon arrival at the laboratory. Infrared thermometers may not be used to measure sample temperatures for microbiological analyses. Sample bottles will be filled sequentially. Bottles will normally be filled to the shoulder of the bottle, leaving a small space for expansion and mixing. VOC bottles will not be intentionally over-filled but carefully filled to achieve a convex meniscus at the top of the bottle, with no air bubbles present; when the VOC lid is screwed on a small volume of water will be displaced and no air will be present in the bottle. EPA guidelines in 40 CFR 136 indicate that samples to be analyzed for dissolved metals must be filtered and preserved with nitric acid within 15 minutes after sample collection. Due to the risk of sample contamination through filtering of metals samples in typical vessel sampling locations in engine room spaces, filtering of dissolved metals will be performed immediately onboard ship using a closed filtration system using cubitainer and sealed pre-cleaned in-line disposable metals filters. A separate ADEC approved standard operating procedure will be provided to sampling staff and lead regulatory personnel that will outline the procedures for metals filtration Note 1: Peristaltic pumps are not to be used for collection of VOC samples due to potential loss of volatile components. Grab Samples Sample bottles will be filled sequentially, normally being filled to the shoulder of the bottle, leaving a small space for expansion and mixing. Note that some sample types such as volatile organic compounds and fecal coliform bacteria have specific bottle filling requirements. The laboratory will provide sampling instructions with the sample bottles. If necessary, samplers will consult with the laboratory regarding sampling procedures. Composite Samples Samples will be composited directly into the sample bottles and collected sequentially. Between composite aliquots, bottles will be kept in a cooler with ice, to reach and maintain a sample temperature of 4 +/-2C. The time of the initial portion of the composite, composite intervals, and the final compositing time will be noted in the field logbook or data sheets. Sample time listed on the Chain-of- Custody or Transmission Form and the sample bottle will be the time of the final sample composite portion. B.3 SAMPLE HANDLING AND CUSTORY REQUIREMENTS B.3.1 Sampling Procedures See Section B.2 of this QAPP Sampling Method Requirements B.3.2 Sample Custody Procedures Samples and sample containers will be maintained in a secure environment, from the time the bottles leave the laboratory until the time the samples are received at the laboratory. The laboratories will maintain custody of bottles and samples using their normal custody procedures. Blind field duplicates will be identified with discrete sampling labels and recorded as blind field duplicates in the sampler's field notebook. To maintain the secure environment for samples on board ship and during transport, samples must be: 1) in the samplers possession (line of sight); or 2) in a cooler sealed with signed and dated friable evidence tape on opposing sides of the cooler; or 3) in a locked cooler for which only the sampler has the key. When the cooler is sealed, the method of securing the samples must be such that tampering with samples or bottles is not possible: The cooler must be secured so that the lid cannot be removed without breaking the evidence tape or cutting the lock, so that tampering would be evident. Transfer of samples will be accomplished using the laboratorys chain of custody form. When samples are transferred between personnel, such transfer will be indicated on the chain of custody form with signature, date and time of transfer. The chain of custody will remain with the samples, sealed inside the cooler, until received by the laboratory. At any time during sample transfer, if custody is broken, a note must be made on the chain of custody form accompanying the sample. Upon receipt at the laboratory, the laboratory sample custodian will make note if a breach of custody has occurred (for example, if a custody seal has broken during transport). B.3.3 Shipping Requirements Samples will be held within the respective method specified sample temperature holding requirements (see Table 8, Sample Containers, Preservations, Holding Times, and Sample Type). A 1 liter temperature blank will accompany all samples and will be measured at the laboratory upon receipt of the samples to verify the temperature. The temperature of this blank will be recorded on the chain of custody upon receipt of the sample at the lab. To maintain the temperature, extra blue ice will be kept frozen on board ship or ship ice will be used. Blue ice or ship ice will be exchanged just before shipment of samples to the lab, and may be exchanged more frequently during the sampling trip, as required. Some samples may be at a temperature near body temperature (37 C) at time of sample collection. This temperature encourages growth of fecal coliform bacteria and thus these samples must be cooled as quickly as possible, without freezing them. These samples shall be placed in a water bath containing ice cubes provided on board ship. The bottles should be immersed in the water to the shoulder, rotated frequently, and ice should be added/water drained off as the ice melts for approximately one hour until the sample reaches a temperature of <10 C. To ensure custody of these samples (the sample bottles may not be able to be sealed in the cooler until the temperature is lowered) these bottles can be sealed with custody tape individually, as necessary. Holding time limitations must be considered when decisions are made regarding sampling and shipping times. Sample holding times are as described in Table 8 above. Planned sample shipping schedules will allow for the meeting of these holding times. The most critical holding time will be that of fecal coliforms, which is defined by EPA as 6 hours from sample collection to laboratory receipt of sample and an additional time of 2 hours from sample receipt at lab to initiating the sample incubation period. Note: these times are distinct and not additive. B.4 ANALYTICAL METHODS AND REQUIREMENTS Laboratories providing analytical support to the Small Vessel Cruise ship Program for water/wastewater samples collected within Alaska at a minimum must meet the specifications found in section A.6.1, Project Description. Monitoring shall be conducted in accordance with EPA-approved analytical procedures and in compliance with 40 CFR Part 136, Guidelines Establishing Test Procedures for Analysis of Pollutants. Reference the Projects MQO table 4 (section A.7.2) of this QAPP for list of parameters of concern, approved analytical methods, method-specific detection and reporting limits, accuracy and precision criteria limits applicable to this project. 40 CFR, Part 136.6 lists other regulated pollutant parameters not listed in Table 4. Only approved methods for water/wastewater (not drinking water) will be used for the analysis of microbiological, chemical and physical measurements. Any lab performing analytical work on samples collected within Alaska must provide (or have on file with the DEC DOW QA Officer) a current electronic copy of their approved Laboratory Quality Assurance Manual (and respective measurement method SOPs to the ADEC Division of Water QA Officer and DEC Project Manager. These documents must specify calibration and quality control (QC) criteria, practices and procedures for each method employed that are essential in the review, validation, and verification and reporting of sample result data. B.5 QUALITY CONTROL REQUIREMENTS Quality Control (QC) is the overall system of technical activities that measures the attributes and performance of a process, item, or service against defined standards to verify that they meet the monitoring projects data quality objectives. For the Small Vessel Cruise ship Monitoring Program QC activities are separated into: Field Quality Control Measures, and Analytical Laboratory Quality Control Measures. In this section define the quality control activities that will be used to control the monitoring process to validate sample data. Use separate tables to define field QC measurements and Lab QC measurement and their criteria for accepting/rejecting project specific water quality measurement data. B.5.1 Field Quality Control (QC) Measures Quality Control measures in the field that will be used to control the monitoring process to validate sample data include but are not limited to: Proper cleaning of sample containers and sampling equipment. Maintenance, cleaning and calibration of field equipment/ kits per the manufacturers and/or laboratorys specifications, and field Standard Operating Procedures (SOPs). Chemical reagents and standard reference materials are used prior to expiration dates. Proper field sample collection and analysis techniques. Correct sample labeling and data entry. Proper sample handling and shipping/transport techniques. Field blind replicate sample (blind to the laboratory) sample (minimum 1 replicate/ship/monitoring season) for each parameter). Field replicate measurements (minimum 1 replicate measurement/ship/monitoring season for each parameter). Field Replicate samples will be sampled/measured at a frequency of one replicate sample per ship per cruise ship operating season for all conventional and priority pollutants. Each ship is required to submit 2 discreet sampling events (all parameters) per monitoring season with one sample event collected a replicate, for a total of 3 samples/monitoring season. Replicate samples collected for subsequent laboratory analysis will be submitted as blind replicate samples to the lab for analysis. QC acceptance criteria for field/lab replicate (precision) samples are listed in Table 4 Section A.7.2 under the precision column. B.5.2 Laboratory Quality Control (QC) Measures Sub-contracted laboratories will provide analytical results after verification and validation by the laboratory QA Officer. The laboratory must provide all relevant QC information (including method/parameter specific QC acceptance criteria limits) with its summary of data results so that the project manager and project QA officer can perform field data verification and validation, and review the laboratory reports. The project manager reviews these data to ensure that the required QC measurement criteria have been met. If a QC concern is identified in the review process, the Project Manager and Project QA Officer will seek additional information from the sub-contracted laboratory to resolve the issue and take appropriate corrective action/s. B.6 INSTRUMENT/EQUIPMENT TESTING, INSPECTIONAND MAINTENANCE REQUIREMENTS Field instruments include a pH test kit, chlorine residual colorimeter instrument, and a calibration /verification thermometer. Maintenance of the chlorine residual colorimeter instrument includes keeping the sample cell rinsed after sample measurement, keeping the cell clean and free of fingerprints and oils, and checking the instrument annually against a known set of standards. An extra cell will be kept with the test kit in case of breakage or scratches to the sample cell. The field kit will be checked against the lab kit at the beginning and end of the sampling season. The traceability of this calibration/verification will be documented in the respective instrument field book. The analysis of pH in the field will be used for reference purposes only and will be verified through laboratory analysis. A pH probe shall be used that ensures the most accurate reading possible in the expected range of pH values. The laboratory will supply pH 4.0, 7.0 and 10.0 reference buffers to the sampling team for field verification along with standard reference traceability documents. Prior to a sampling event, all sampling instruments and equipment are to be tested and inspected in accordance with the manufacturers specifications. All equipment standards (thermometers, barometers, etc.) are calibrated appropriately and within stated certification periods prior to use. Monitoring staff will document that required acceptance testing, inspection and maintenance have been performed. Records of this documentation should be kept with the instrument/equipment kit in bound logbooks or data sheets. B.7 INSTRUMENT CALIBRATION AND FREQUENCY Field instruments include a pH test kit, chlorine residual colorimeter instrument, and a thermometer. Maintenance of the chlorine residual colorimeter instrument includes keeping the sample cell rinsed after sample measurement, keeping the cell clean and free of fingerprints and oils, and checking the instrument annually against a known set of standards. An extra cell will be kept with the test kit in case of breakage or scratches to the sample cell. The field kit is to be checked against the lab kit once per season. The calibration of these instruments will be documented in field books. Field instruments shall be calibrated where appropriate prior to using the instruments. For example, pH meters shall be calibrated according to the manufacturers specifications using pH buffers at 4.0, 7.0 (mid-range) and 10.0 that are within their certification period (expiration date has not lapsed). If equipment and/or kits require calibration immediately prior to the sampling event, the calibration date will be recorded in the operators field logbook or field data sheets. When field instruments require only periodic calibration, the record of this calibration should be kept with the instrument. B.8 INSPECTION/ACCEPTANCE OF SUPPLIES AND CONSUMABLES Sample bottles will be visually inspected prior to sampling. If problems with bottles are noted, such as a cap that has fallen off an empty bottle, note of the problem will be made on the chain of custody form. Spare parts will be available for all equipment used and Standard Reference Materials and test kit reagents used in sampling will be checked to ensure that they are within expiration dates. All reagents, calibration standards, and kit chemicals are to be inspected to ensure that expiration dates have not been exceeded prior to use in the monitoring project. All sample collection devices and equipment will be appropriately cleaned prior to use in the monitoring project. All sample containers, tubing, filters, etc. provided by a laboratory or by commercial vendor, will be certified clean for the analyses of interest. The sampling manager/person will make note of the information on the certificate of analysis that accompanies sample containers to ensure that they meet the specifications and guidance for contaminant-free sample containers for the analyses of interest. No standard solutions, buffers, or other chemical additives will be used if the expiration date has expired. It is the responsibility of the sampling manager or his/her designee to keep appropriate records, such as logbook entries or checklists, to verify the inspection/acceptance of supplies and consumables, and restock these supplies and consumables when necessary. Contracted and sub-contracted laboratories will follow procedures in their laboratorys QAP and SOPs for inspection/acceptance of supplies and consumables. B.9 DATA MANAGEMENT The success of a monitoring project relies on data and their interpretation. It is critical that data be available to users and that these data are: Of known quality, Reliable, Aggregated in a manner consistent with their prime use, and Accessible to a variety of users. Data Management includes accurate field notebook entries, completed Chain-of-Custody forms and laboratory data management documents. Laboratory data management procedures and processes are described in the respective Laboratory's Quality Assurance Plan. This document must be available upon request. The Vessel Representative will report data directly to the ADEC Project Manager after thorough review by the sampling manager and the laboratory QA Manager within the regulatory time limits. Quality Assurance/Quality Control (QA/QC) of data management begins with the raw data and ends with a defensible report, preferably through the computerized messaging of raw data. C.1 ASSESSMENTS AND RESPONSE ACTIONS C.1.1 Field Assessments ADEC may perform a field sampling audit on randomly chosen sampling events during the season in order to evaluate the performance of the samplers. Follow-up field audits may be necessary pending audit findings. Audits will concentrate on sampling technique, sample handling, field records, field testing methods, and adherence to vessel specific sampling plans and the QAPP. ADEC will send these audit reports to the responsible vessel operator within 14 days of the audit. These reports will include corrective actions, if necessary. C.1.2 Laboratory Assessments Laboratories are subject to periodic and extensive audits by regulatory agency personnel as part of their certification. Reports of these audits will be made available to the ADEC Project Manager and ADEC Water Quality Assurance Officer. The ADEC project manager may review any recent and pertinent technical systems audit reports of the analytical laboratories involved in this project. C.1.3 Replicates One blind conventional and priority replicate sample will be collected for each vessel each cruise ship monitoring season. Acceptance criteria for blind replicate samples will be the same as the method specific MQO precision criteria (Table 4). C.1.4 Corrective Action The laboratory or sampling manager will notify the ADEC Project Manager and Vessel Representative, if errors are noted by the laboratory or sampling personnel. The responsible party will then immediately correct the problem and will send those corrections via email to the Vessel Representative and ADEC Project Manager and the ADEC DOW QA Officer. C.2 REVISIONS TO QAPP Annually the QAPP will be reviewed and revised as needed. Minor revisions may be made without formal comment. Such minor revisions may include changes to non-signatory identified project staff, QAPP distribution list and/or minor editorial changes. The QAPP will be approved for three years. At that time a new QAPP will be submitted for ADEC approval. Revisions to the QAPP that affect stated monitoring Data Quality Objectives, Method Quality Objectives, method specific data validation critical criteria and/or inclusion of new monitoring methods must solicit input/ and pre-approval by DEC DOW QA Officer/DEC Project Management before being implemented. D. DATA VALIDATION AND USABILITY D.1 DATA REVIEW, VERIFICATIONAND VALIDATION REQUIREMENTS During the overall small ship sampling project, the ADEC Project Manager or their designee will review field notes and laboratory data packages to detect correctable problems for the remainder of the study. Upon receipt of these completed data packages from the Vessel Representative the ADEC Project Manager or designee will review data and field notes to verify that this QAPP was followed. Items reviewed will include: Comparison of dated vessel specific sampling plans with the QAPP to assure that the correct samples were taken. Comparison of dated sampling plans with field notes and custody forms to assure that planned samples were collected. Review of field notes and data to assure that information specified in the QAPP has been recorded. Review of laboratory data packets, particularly the QA/QC laboratory sheets. Any problems noted must be immediately brought to the attention of the Vessel Representative who will notify the Lab Manager or sampler who will take appropriate corrective action as necessary. D1.1 Data validation Data validation means determining if data satisfy QAPP-defined user requirements; that is, that the data refer back to the overall data quality objectives. Data validation is an analyte and sample-specific process that extends the evaluation of data beyond method, procedural, or contractual compliance (i.e., data verification) to determine the analytical quality of a specific data set to ensure that the reported data values meet the quality goals of the environmental data operations (method specific data validation criteria). D1.2 Data Verification Data Verification is the process of evaluating the completeness, correctness, and conformance/compliance of a specific data set against the method, procedural, or contractual requirements. D1.3 Data Review Data Review is the process that evaluates the overall data package to ensure procedures were followed and that reported data is reasonable and consistent with associated QA/QC results. D.2 VERIFICATION AND VALIDATION METHODS D2.1 Validation Methods Data validation determines whether the data sets meet the requirements of the project-specific intended use as described in the QAPP. That is, were the data results of the right type, quality, and quantity to support their intended use? Data validation also attempts to give reasons for sampling and analysis anomalies, and the effect that these anomalies have on the overall value of the data. All data generated shall be validated in accordance with the QA/QC requirements specified in the methods and the technical specifications outlined in this QAPP. Raw field data will be maintained by the Program staff who collect it. Raw laboratory data shall be maintained by the laboratory. The laboratory may archive the analytical data into their laboratory data management system. All data will be kept a minimum of 5 years. The summary of all laboratory analytical results will be reported to the Project supervisor/manager staff. Data validation will be performed by the laboratory for all analyses prior to the release of data. All laboratory data will be validated according to the laboratorys QA Manual and SOPs and as specified in the Monitoring Projects QAPP. The rationale for any anomalies in the QA/QC of the laboratory data will be provided to the Project Manager with the data results. Completed Chain-of-Custody or Transmission forms (if required) will be sent back from the laboratory to the Project Manager. D2.2 Verification Methods The primary goal of verification is to document that applicable method, procedural and contractual requirements were met in field sampling and laboratory analysis. Verification checks to see if the data were complete, if sampling and analysis matched QAPP requirements, and if Standard Operating Procedures (SOPs) were followed. Verification of data is the responsibility of the Project QA Officer. The Project QA Officer should verify at least 10% of generated project data. D.3 RECONCILIATION WITH USER REQUIREMENTS The Project Manager and the Project QA Officer will review and validate data against the Projects defined MQOs prior to final reporting stages. If there are any problems with quality sampling and analysis, these issues will be addressed immediately and methods will be modified to ensure that data quality objectives are being met. Modifications to monitoring will require notification to ADEC and subsequent edits to the approved QAPP. Only data that have been validated and qualified, as necessary, shall be provided to ADEC Division of Water and entered into the applicable database. Appendix A - Alaska Small Cruise Ship Sampling Checklist Vessel NameDateSample Type (Source)Sampler Name(s)Sample NumberNote: Applicable Calibration records to be included in the field notes. Notification: ADEC project manager notified 36 hours prior to the sampling event. Type of Sampling Conventional pollutants only. Conventional and priority pollutants. Other- (BMP Approval/Compliance Required Sample/etc.) Samples will reflect a representative discharge of treated graywater and graywater/blackwater combinations into applicable waters from an operable marine sanitation device or other treatment system. Second sample event will be at least 21 days after the first sampling event. Ready Equipment Check that equipment operates, and that calibration is up-to-date Digital Thermometer pH Meter Cl Meter Make sure that equipment identification numbers and dates of last calibration are included in the sample field notes. Protective Equipment- check condition and availability Goggles Gloves Clothing Sampling Bottles Inventory Sampling Bottles Bottles are pre-cleaned and will not require rinsing with sample. When sample bottles are pre-preserved, bottles will never be rinsed but will be filled only once with sample. Fill out Custody Form based on type of sampling being conducted. Samples will be listed as grab on the COC form. Sampling Identification Sample ID should clearly state where the sample was taken. For example, a mixed black and gray sample taken from the MSD discharge line is MSD BW&GW as its sample ID. Source of the WW should be identified. For example- mixed galley and laundry graywater should be identified as GW Mixed- Laundry and Galley. If sample is taken from a tank- identify the tank matching the naming conventions in the VSSP. Fill out bottle labels Verify Sampling Site Sample ports within 50 feet of the point of overboard discharge. Determine what sampling sites are to be used for the event. Ensure functioning of sampling port. Conduct a pre-sampling reading of Cl and pH Review VSSP and BMP Specific sampling techniques for each vessel will be detailed in the VSSP. Determine any deviation from submitted plans. Pre sampling brief with vessel Master Confirm location and time of sampling. Discuss documentation of sampling event and Alaska regulations regarding location and speed. Discuss any deviations from submitted plans. Discuss sampling time line. Sampling Field Notes will include: Vessel name Names of sampling personnel, names of shipboard assistants Signature or initials of the sampler indicating that the sample port is correct. Sample ID clearly stating where the sample was taken. Sample date and times recorded on COC. Records collected on discharge flow rates. Nature of sample recorded (composite or grab). Waste type recorded (blackwater, graywater, or mixed) If deviations from VSSP and/or QAPP noted, reported to ADEC. If unannounced sampling, sampler verified that vessel is discharging. Latitude/longitude and speed at time of discharge being sampled is recorded (only for underway sampling), Copy of the Discharge record for the sampled discharge included. COC properly completed Samples delivered to laboratory within holding times for analyses. Maximum of 6 hours. Sampling event Care will be taken to assure sample representativeness and homogeneity. Pre-sampling assembling of equipment Bucket for flushing system Cooler filled with ice water. Field testing equipment Pad of paper/Pen Protective Procedures Samplers will wear disposable gloves and safety eyewear. Observe precautions while collecting samples, remaining aware of the potential biohazard present Samplers will take care not to touch the insides of bottles or lids/caps during sampling. Sampling Procedures Record time, Latitude, Longitude, waste sample, sample location, Flushing a volume of water equal to at least ten times the volume of the sample discharge line will first be discharged into a bucket or similar container to clear the line of standing water and possible contamination. Commence sampling event with Field Test Check Chlorine and record pH, and temperature in the field notebook VOC bottles will be filled leaving a convex meniscus at the top of the bottle, with no air bubbles present; when the VOC lid is screwed on a small volume of water will be displaced and no air will be present in the bottle. With the exception VOC bottles, all bottles are normally filled leaving a small space for expansion and mixing. Samples will be cooled immediately in an ice-water bath to 4 C and then placed into a cooler containing frozen blue ice or ice and water mixture to maintain a sample temperature of 4 +/-2 C. Secure cooler for transport to laboratory with custody seals. The cooler must be either secured or under observation to ensure it is not tampered with. Record on COC form each time the cooler changes custody. Each person receiving needs to sign and date with time of transfer. Samples delivered to laboratory within holding times for analysis. The cooler must be either secured or under observation to ensure it is not tampered with. Record on COC form each time the cooler changes custody. Appendix B - Alaska Small Cruise Ship Data Review Checklist Vessel Name ______________________________________________ Date_____________________________________________________ Location__________________________________________________ Sampling Team____________________________________________ Laboratory_______________________________________________ Sample Type: BMP sample Event Event 1 / Field parameters Conventional I (shortlist). See Approved VSSP document Event 2 / Field parameters Conventional II. See Approved VSSP document Nutrients sampling (combined with event 1 or 2) (if applicable). See Approved VSSP document Priority (combined with Event 1 or 2). 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This narrative should explain when results are outside the precision and accuracy limits and the corrective actions taken to rectify QC problems. Chain of custody form Cooler receipt forms with temperature indicated Discharge logs covering time of sampling Field notes. Latitude and longitude information pertaining to each sample including which overboard port the waste was discharged through and the speed the vessel was traveling Completed sampling checklist Completed data review checklist Appendix C  QAPP Deadlines* *Please Note- these deadlines can change due to regulatory or statute changes. Please consult the latest ADEC regulations. Applications: QAPP: March 1st VSSP: 21 days before sampling (ADEC). Preferable to provide VSSP application well in advance of this date, this to allow for ADEC CPVEC staff timely to review. Sampler qualifications: 21 days before sampling. Notifications: Deviations from VSSP: Immediately. Deviations from BMP Plan: Immediately. Sample Event- 36 hours prior. Audit event: 36 hours prior. Errors noted by lab or samplers: 7 days Non-Compliance: within 24 hours of discovery. Actions taken by the vessel to avoid re-occurrence: immeadelety after discovery / See BMP plan. Analytical Reports: 21 days after completion of lab analysis. Audits Initial audit: Within 30 days of project initiation. Second audit: Midway through the project. Reporting: Sampler training/certification information: when requested. Sample scheduling, date, time, and location. At the beginning of the season (reference VSSP approval process) Sample Value exceedance: Report immeadeteley when discovery notification of the lab. See BMP procedure. Field instruments calibration and certification: Due May 31st and July 31st. Audit reports due within 14 days. Draft reports are due with 7 days. Technical laboratory audits: Draft due 1-2 weeks of end audit, final due 2-4 weeks of end audit. First data review: Due by June 15th Other data reviews: equally spaced through the season Data review with problems noted: Immediately notify, then 40 days to submit report after sample event. Appendix D VSSP Blank Copy Please contact DEC for the most up-to-date version.  The VSSP for each vessel will list the proper location and timing of wastewater sampling. The samples will be taken in a manner that seeks to capture a typical wastewater discharge while still meeting the fecal coliform 6-hour holding time.     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