ࡱ>  5@ DDbjbj22 +XXm7~~~|6$0(f^sss'''''''$)Rn+'~e!se!e!''&&&e!(~'&e!'&&3&H6~3&Z ye#&3&'(00(3&,'%,3&,~3&|s,&u!Dsss'' v D&v Generic Head Models for Atlas-Based EEG-Source Analysis Darvas, F and Leahy, R.M. University of Southern California, Los Angeles, CA, USA Corresponding Author: Felix Darvas, 3740 McClintock Ave. #408, Los Angeles, CA 90089, USA, Fax (213) 7404651, Phone (213) 7404143, darvas@sipi.usc.edu ABSTRACT We describe a method for using a generic head model to produce EEG source localizations and give an assessment of the errors associated with the use of this model. The model is derived from surface landmarks of the individual by a non-rigid warp of an atlas brain. Sources are localized in the warped atlas and mapped back to the original atlas. This approach allows comparing source localizations across subjects in an atlas-based coordinate system, which can be used in the large fraction of EEG studies where MR images are not available. We evaluate this method by investigating the localization errors in subject and atlas coordinates. The Montral phantom was used as atlas. The phantom was fitted to the individual head by a TPS warp, using a 155-electrode configuration as landmarks. Dipolar sources were placed in the phantom and transferred to the individual by an anatomical feature based warp, thus ensuring that sources were placed at the same anatomical location. Data were simulated in the subject and a dipole fit was performed, using a FEM of the warped phantom and the localized sources were transferred to the original phantom and compared to their original position. We simulated 972 locations, evenly distributed over the white-matter surface of the phantom. The error was estimated for 10 subjects and we found a mean error of 15.2 mm in atlas space and 8.0 mm in subject space. With a three-shell spherical model, errors were 27.2 mm in subject space and 34.7 mm in atlas space. KEY WORDS Montral brain phantom, radial basis function, warping, EEG forward models, source localization INTRODUCTION Multi-channel electroencephalography (EEG) is a widely available and inexpensive method for measuring functional information about the human brain. The measured electric potentials can be used to determine the location and strength of neural sources by means of an inverse procedure. These sources are modeled as equivalent current dipoles. Ideally, individual anatomical MR scans of the subject are used, both for defining the forward model that relates source location and strength to the measured scalp potentials. However in practice, EEG studies are often performed without accompanying anatomical scans. Here we describe a procedure in which surface landmarks are used to warp an atlas to the subject's scalp. The warped atlas is used to define a forward model for source localization. The source locations are then mapped back to the standardized atlas using an inverse warp. In this way, we can infer the locations in cortical anatomy of EEG sources without an individual's MR image. Furthermore, the warping procedure allows the use of a standardized coordinate system in the original atlas space for inter-subject studies. Solution of the EEG inverse problem requires the solution of an associated electromagnetic forward problem, which yields the scalp potential distribution for a given source [Mosher, 1999]. While spherical models exist for EEG and can be used for source reconstruction, realistic head-models, derived from the individual subjects head anatomy, yield an increased accuracy of source localization [Buchner, 1996, Leahy, 1998]. The finite element method (FEM) can be used to calculate the forward model from a realistic model and dipole source locations can then be determined by combining this forward model with inverse procedures based on least squares [Scherg, 1990] or signal subspace approaches [Mosher, 1998]. The use of a standardized head-model offers a compromise between individual models and the oversimplifying sphere model. The surface electrode locations in an individual geometry can be readily measured using an inexpensive 3D spatial localization device and a nonlinear mapping can be used to warp a generic head to match the individual surface. Here we achieve this using a thin-plate spline (TSP) fitting procedure [Ermer, 2001] in which the electrode placement is constrained to follow a regular pattern based on the 10-20 system [Jasper, 1958], where electrode positions serve as landmarks in both geometries. The fitting procedure produces a warping of the 3D coordinates of the generic head to match the subjects scalp at the electrode locations and we use the warped atlas to define and solve the forward problem and inverse problem. A meaningful interpretation of these results requires that they be referenced to the subjects neuroanatomy. Since this is not available, instead we apply an inverse of the thin plate spline warp and view the sources with respect to a generic stereotactic coordinate system defined by the original atlas. We present a quantitative analysis of the generic head modeling approach using the Montreal Brain Phantom [Collins, 1998] as generic atlas and generated landmarks from individual geometries, which in turn were based on MR images of 10 volunteers. We used a 155 electrode extension of the 10-20 system with placement determined by the locations of the nasion and left and right preauricular points and computed errors in localization of sources at a large number of points distributed over the entire cortex for each subject. To allow comparison across subjects, the cortex in each individual was defined by first matching the atlas brain to each of the subject brains with a nonlinear intensity based-warp using the AIR (automated image registration) software package [Woods, 1992]. We then mapped points on the atlas cortex to their corresponding locations in each subject. For each of these we computed the forward field from individual head models. The sources were then localized using RAP-MUSIC [Mosher, 1998] in the warped atlas. These results were then used to compute the localization errors in individual subject coordinates. Using an inverse warp, we then mapped the locations back to the atlas and computed average localization errors and standard deviations in the atlas coordinate system. For comparison, we also computed localization errors for inverse procedures based on a three shell spherical model. METHODS A summary of generating the generic model is outlined in Fig. 1. We generated a generic 155-electrode configuration for the phantom and the realistic geometries, using an extension of the 10-20 system, with 5/10% intervals between electrodes instead of 10/20% intervals. The finite element method was used to compute the forward models, which were used to simulate sources in the individual head geometry and to localize dipoles in the warped atlas. We used the sourceless dipole approach which has been described by [Awada, 1997] and [Marin, 1998]. By use of this method, data, as a function of an arbitrary point source can be generated. Likewise, the FEM can be used to reconstruct sources in a realistic head model. THE TSP WARP  If only sparse surface information about the subject geometry is available, the TPS - warp provides method to adapt the generic model to the individual head geometry. The only input required for this warp is a set of landmarks or electrode positions on the surface of the head. A requirement is that these landmarks match the respective landmarks on the phantom. A simple way to generate these landmarks is to use the standard electrode configuration on the subject. The TPS uses coordinate transformations based on radial basis functions, which have a number of advantages over affine or polynomial transformation [Carr, 1997]. The warp can be split in two parts,  EMBED Equation.3  an affine transformation of the position vector rA and a non-linear transformation f(rA). The affine transformation and the function f have the set of L landmarks lA as parameters. The subscripts indicate that a warp matching the phantom-coordinate system to the realistic subject's coordinates is performed. The function f(rI) is defined by  EMBED Equation.3  with the condition that  EMBED Equation.3 . The non linear transformation depends only on the distance of a location to the landmarks and vanishes, as this distance goes to infinity. Therefore, the deformation is local, and points, which are far away from the landmarks, are only subject to the affine transformation. Also, the transformed landmarks match exactly. For our simulations we transformed the phantom-coordinates by means of the TSP warp, in order to align the two geometries For the transformation back into atlas coordinates we used the inverse warp, i.e. the warp based on landmarks in the individual geometry (using lI instead of lA)  EMBED Equation.3 . This is not exactly the inverse to warp from phantom to individual, but in the case of small distortions, the error  EMBED Equation.3  can be expected to be small. SIMULATION The method was evaluated using data simulated from 10 individual subjects using anatomical T1-weighted MR images of the head. For each subject we generated a set of cortical dipoles and simulated EEG potentials corresponding to each of these dipoles using the FEM based on the individual geometry. In order to estimate the anatomical localization error in the phantom, the simulated source positions in the subjects geometry have to be at the same anatomical location as they are in the phantom. This can be achieved if one uses an image based warping procedure that maps the phantom brain onto the individual brain. We used the AIR-package to find the polynomial warp from each of the 10 individual subjects brain images to the Montral phantom. We generated sources at 972 positions on the white matter surface of the phantom. Each of these sources was transferred to the individual subject coordinate system, by applying the polynomial coordinate transformation to its coordinates in the phantom space. From the warped phantom a forward model was computed for use in the source reconstruction. We used RAP-MUSIC, to localize the dipole positions in the warped phantom space and applied the inverse warp to obtain the reconstructed atlas positions of the sources. These can then be compared with the original simulated positions and the anatomical localization error can be computed as the difference between simulated and reconstructed location. The whole simulation is outlined in Fig.2. RESULTS  Figure 3 shows the anatomical localization error for all ten subjects. The error was averaged over the whole white-matter surface (972 locations). The maximum error was 27.3 mm for subject 4, the minimum error was 8.0 mm for subject 2. The overall mean anatomical localization error was 15.2 mm with a standard deviation (s.d.) of 5.9 mm. Figure 4 shows the results for the same procedure with a 3-shell sphere fitted to the individual electrodes, which replaced the warped phantom as forward model. The error was averaged over the whole white-matter surface (972 locations). The maximum error was 49.2 mm for subject 8 the minimum error was 25.6 mm for subject 10. The overall mean anatomical localization error was 34.7 mm with a s.d. of 6.6 mm. DISCUSSION The use of a realistic phantom instead of a spherical model can significantly improve the localization error, as shown in Fig. 3, although these errors are still quite large. One source, which contributes to this error, is not the mismatch in the electromagnetic forward model and subsequent localization, but in the pure geometrical transfer error, which arises from the fact, that the TSP-warp is not an exact inverse for the anatomical feature-based polynomial warp. If only the error in the individual subject space is considered, the errors are smaller for both cases, sphere and phantom and drop to 8.1 mm and 27.2 mm. These are comparable to errors reported for sphere and a generic BEM (boundary element model) by Fuchs et. al in 2002. However, while this error is useful for reconstructions in individual geometries, for comparisons of solutions in a common coordinate system, the error in the original phantom space has to be considered. REFERENCES Awada KA and Jackson DR et al. Computational Aspects of Finite Element Modeling in EEG Source Localization. IEEE Trans. on Biomed. Eng.1997;44:736-752 Buchner H and Waberski TD et al. Comparison of realistically shaped boundary element and spherical head models in source localization of early somatosensory evoked potentials. Brain Topography. 1996; 8:137-143 Carr JC and Fright WR et al. Surface Interpolation with Radial Basis Functions for Medical Imaging. IEEE Trans. Med. Imag.1997;16: 96-107 Collins DL and Zijdenbos AP et al. Design and construction of a realistic digital brain phantom. IEEE Trans. Med. Imag. 1998;17:463-468 Ermer JJ and JC Mosher et al. Rapidly recomputable EEG forward models for realistic headshapes. Physics in Medicine and Biology. 2001;46:1265-1281 Fuchs M and Kastner J et al. A standardized boundary element method volume conductor model. Clinical Neurophysiology. 2002;113:702-712 Jasper HH. The Ten Twenty Electrode System of the International Federation. Clinical Neurophysiology. 1958;10:371-375 Leahy RM and Mosher JC et al. A study of dipole localization accuracy for MEG and EEG using a human skull phantom. Clinical Neurophysiology. 1998;107:159-173 Marin G and Guerin C et al. Influence of skull anisotropy for the forward and inverse problem in EEG: simulation studies using FEM on realistic head models. Human Brain Mapping. 1998;6:250-269 Mosher JC and Leahy RM. Recursive MUSIC: A framework for EEG and MEG source localization. IEEE Trans. on Biomed. Eng. 1998;45:1342-1355 Mosher JC and Leahy RM et al. EEG and MEG: forward solutions for inverse methods. IEEE Trans. on Biomed. Eng. 1999;46:245-259 Scherg M. Fundamentals of dipole source potential analysis. Advances of Audiology. 1990;6:40-69 Woods RP and Cherry SR et al. Rapid automated algorithm for aligning and reslicing PET images. Journal of Computer Assisted Tomography. 1992;16:620-633 Figure  SEQ Figure \* ARABIC 1: Outline of the modeling using a generic head model. The coordinates of the generic model or the atlas are denoted as rA, the individual coordinate system is denoted as rI. The model properties of the atlas are given by (( rA) A, while (I is unknown. The atlas coordinates are transformed into the individual coordinate system by means of the TPS-warp. The forward model is calculated with the warped model property ((rA) I and sources at rI Source are localized in the warped atlas coordinates. In order to obtain the coordinates of the source in atlas coordinates, the resulting source localization is warped from individual coordinates to atlas coordinates by an inverse warp transformation. Figure  SEQ Figure \* ARABIC 2: Outline of the simulation to evaluate the use of the generic model. A source is placed in the generic model and warped to the individual coordinate system by a brain-feature based warp. The anatomical location is the same in the individual brain as in the atlas. After a source position has been generated, the generic modeling and reconstruction procedure is applied. Figure 3: The mean anatomical localization error for the warped phantom and sphere for 10 subjects. 89:@HOT" # $ , . 3 PW;zrgr[rShPCJaJh(h(5CJaJh(h(CJaJh(CJaJh(hL5CJaJhLCJaJh0 CJaJh0 h0 CJaJh$ CJaJh$ 5CJaJh$ h$ 5CJaJ hWhWhr+wCJaJhr+whr+wCJaJhr+wh)jCJaJh)jCJaJhVCJaJh)jhW5CJaJ8:T# $ - . 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