ࡱ> *'` 0bjbjLULU x.?.? " " " " &&&t&PPPd |E&\4,`0 :=t$@hP˝&Iz/~/lII˝ " "h4sssI "&sIssu$&[( PPYbWHm f',[[&&0P?NB\sDFP?P?P?˝˝XP?P?P?IIII&&&ĝP&&&P&&& " " " " " " Guidelines and specifications for managing TB laboratory equipment and supplies Acknowledgements This document was developed by: Knut FeldmannArmand Van DeunGrace KahenyaVronique VincentCatherine Mundy We acknowledge the contributions of: Mohamed Abdel AzizKarin WeyerJean de Dieu IragenaMarija JoncevskaChris GilpinJessica MinionSoleil LabelleMark PerkinsJohn LoeberJavid SyedCN ParamasivanAdithya CattamanchiJohn RidderhofCatharina BoehmeMarina ShulginaMoses JolobaWilly UrassaRichard BumgarnerFuad MirzayevThe Global Laboratory Initiative gratefully acknowledges the financial support received from the United States Agency for International Development (USAID) through the Tuberculosis Control Assistance Program (TBCAP), under the terms of cooperative agreement number GHS-A-00050001900, to develop and publish this document. Acronyms AampereAC alternating currentAFBacid-fast bacilliAIDSacquired immunodeficiency syndromebpbase pairBSCbiological safety cabinetCdegrees CelsiusCAaverage monthly consumptionCFCchlorofluorocarbonCIcolour indexdbAdecibel (A-scale weighted)DCdirect currentDNAdeoxyribonucleic acidDNasedeoxyribonucleaseDOTSdirectly observed treatment, short-courseDSTdrug susceptibility testingEDTAethylenediaminetetraacetic acidENEuropean norms (standards)ETFEethylene tetrafluorethyleneFEFOfirst expired, first outFIFOfirst in, first outFL-DSTfirst-line drug susceptibility testingFMfluorescence microscopeGLPgood laboratory practiceHDPEhigh-density polyethyleneHEPAhigh-efficiency particulate airHFChydrofluorocarbonHIVhuman immunodeficiency virusHQheadquartersHzhertz (frequency)IECInternational Electrotechnical Commission ISOInternational Organization for StandardizationkWkilowattLCDliquid crystal displayLDPElow density polyethyleneLEDlight-emitting diodeLJLoewensteinJensenSmicrosiemensMGITMycobacteria growth indicator tubeMTBMycobacterium tuberculosisMWmolecular weight [g/mol]NAnumerical aperture NALCN-acetyl-L-cysteineNGOnongovernmental organisationNRLnational reference laboratoryNSFNational Sanitation Foundation (standards, United States)NTPnational tuberculosis control programmep.a.per analysisPaspascal second (SI derived unit of dynamic viscosity)PCRpolymerase chain reactionPEpolyethylenePNBparanitrobenzoic acidPPpolypropylenePTFEpolytetrafluorethylenePVCpolyvinyl chloridercf relative centrifugal forceRNaseribonucleaserpm revolutions per minuteSsiemens (unit of conductivity)SL-DSTsecond-line drug susceptibility testingSOPstandard operating procedureTBtuberculosisTBCAPTuberculosis Control Assistance Program (funded by USAID)TPXpoly-4-methylpentene-1TWB/TWWdigitally adjustable electronic temperature controllerUNUnited NationsUPSuninterrupted power supply USAIDUnited States Agency for International DevelopmentUSDUnited States dollarUVultravioletUVGIultraviolet germicidal intensityVvoltWwattWHOWorld Health OrganizationZNZiehlNeelsen Contents  TOC \h \z \t "Heading 1,2,Heading 2,3,PartTitle,1"  HYPERLINK \l "_Toc275942721" Acknowledgements  PAGEREF _Toc275942721 \h ii  HYPERLINK \l "_Toc275942722" Acronyms  PAGEREF _Toc275942722 \h iii  HYPERLINK \l "_Toc275942723" Contents  PAGEREF _Toc275942723 \h v  HYPERLINK \l "_Toc275942724" Overview  PAGEREF _Toc275942724 \h 7  HYPERLINK \l "_Toc275942725" Scope and content  PAGEREF _Toc275942725 \h 7  HYPERLINK \l "_Toc275942726" Purpose and objectives  PAGEREF _Toc275942726 \h 7  HYPERLINK \l "_Toc275942727" Structure of document  PAGEREF _Toc275942727 \h 8  HYPERLINK \l "_Toc275942728" Part A Guidelines for countries on procuring and managing laboratory equipment, supplies and other commodities  PAGEREF _Toc275942728 \h 9  HYPERLINK \l "_Toc275942729" A1 Introduction  PAGEREF _Toc275942729 \h 10  HYPERLINK \l "_Toc275942730" A1 Introduction  PAGEREF _Toc275942730 \h 10  HYPERLINK \l "_Toc275942731" A2 Laboratory commodity management systems  PAGEREF _Toc275942731 \h 11  HYPERLINK \l "_Toc275942732" A2.1 What is commodity management?  PAGEREF _Toc275942732 \h 11  HYPERLINK \l "_Toc275942733" A2.2 Selection  PAGEREF _Toc275942733 \h 12  HYPERLINK \l "_Toc275942734" A2.3 Procurement  PAGEREF _Toc275942734 \h 14  HYPERLINK \l "_Toc275942735" A2.4 Distribution  PAGEREF _Toc275942735 \h 17  HYPERLINK \l "_Toc275942736" A2.5 Store management  PAGEREF _Toc275942736 \h 19  HYPERLINK \l "_Toc275942737" A2.6 Storage and safe disposal  PAGEREF _Toc275942737 \h 20  HYPERLINK \l "_Toc275942738" A2.7 Rational use of TB laboratory commodities  PAGEREF _Toc275942738 \h 22  HYPERLINK \l "_Toc275942739" A3 Equipment management  PAGEREF _Toc275942739 \h 24  HYPERLINK \l "_Toc275942740" A3.1 National equipment policy  PAGEREF _Toc275942740 \h 24  HYPERLINK \l "_Toc275942741" A3.2 Criteria for selecting equipment  PAGEREF _Toc275942741 \h 24  HYPERLINK \l "_Toc275942742" A3.3 Acquisition  PAGEREF _Toc275942742 \h 24  HYPERLINK \l "_Toc275942743" A3.4 Installation  PAGEREF _Toc275942743 \h 25  HYPERLINK \l "_Toc275942744" A3.5 Validation and calibration  PAGEREF _Toc275942744 \h 26  HYPERLINK \l "_Toc275942745" A3.6 Maintenance and troubleshooting  PAGEREF _Toc275942745 \h 26  HYPERLINK \l "_Toc275942746" A3.7 Professional service and repair  PAGEREF _Toc275942746 \h 26  HYPERLINK \l "_Toc275942747" A3.8 Retiring equipment  PAGEREF _Toc275942747 \h 26  HYPERLINK \l "_Toc275942748" A3.9 Equipment maintenance programmes  PAGEREF _Toc275942748 \h 27  HYPERLINK \l "_Toc275942749" A3.10 Equipment management oversight  PAGEREF _Toc275942749 \h 27  HYPERLINK \l "_Toc275942750" A3.11 Donations of laboratory equipment and other commodities  PAGEREF _Toc275942750 \h 27  HYPERLINK \l "_Toc275942751" A4 Monitoring and evaluation  PAGEREF _Toc275942751 \h 29  HYPERLINK \l "_Toc275942752" A4.1 Monitoring  PAGEREF _Toc275942752 \h 29  HYPERLINK \l "_Toc275942753" A4.2 Evaluation  PAGEREF _Toc275942753 \h 29  HYPERLINK \l "_Toc275942754" A4.3 Indicators for monitoring and evaluation  PAGEREF _Toc275942754 \h 29  HYPERLINK \l "_Toc275942755" Annex A1 AFB-SuppliesProcurement workbook  PAGEREF _Toc275942755 \h 30  HYPERLINK \l "_Toc275942756" Annex A2 CU&DST-Supplies workbook  PAGEREF _Toc275942756 \h 35  HYPERLINK \l "_Toc275942757" Annex A3 Examples of inventory control forms  PAGEREF _Toc275942757 \h 42  HYPERLINK \l "_Toc275942758" Annex A4 Additional resources  PAGEREF _Toc275942758 \h 53  HYPERLINK \l "_Toc275942759" PART B Specifications for TB laboratory equipment, supplies and other commodities  PAGEREF _Toc275942759 \h 55  HYPERLINK \l "_Toc275942760" B1 Introduction to specifications  PAGEREF _Toc275942760 \h 56  HYPERLINK \l "_Toc275942761" B1.1 Layout of lists  PAGEREF _Toc275942761 \h 56  HYPERLINK \l "_Toc275942762" B1.2 Layout of detailed specifications  PAGEREF _Toc275942762 \h 56  HYPERLINK \l "_Toc275942763" B1.3 Manufacturers certificate  PAGEREF _Toc275942763 \h 57  HYPERLINK \l "_Toc275942764" B1.4 Additional headings  PAGEREF _Toc275942764 \h 57  HYPERLINK \l "_Toc275942765" B1.5 Adjustment to local needs  PAGEREF _Toc275942765 \h 58  HYPERLINK \l "_Toc275942766" B2 Equipment, consumables and chemicals for microscopy laboratories  PAGEREF _Toc275942766 \h 59  HYPERLINK \l "_Toc275942767" B3 Main specifications for equipment for culture, DST and molecular biology  PAGEREF _Toc275942767 \h 67  HYPERLINK \l "_Toc275942768" B4 Main specifications for consumables for culture, DST and molecular biology  PAGEREF _Toc275942768 \h 79  HYPERLINK \l "_Toc275942769" B5 Main specifications for chemicals for culture, DST and molecular biology  PAGEREF _Toc275942769 \h 84  HYPERLINK \l "_Toc275942770" B6 Detailed specifications for selected items  PAGEREF _Toc275942770 \h 90  HYPERLINK \l "_Toc275942771" B7 Detailed technical specifications for molecular biology techniques  PAGEREF _Toc275942771 \h 168  HYPERLINK \l "_Toc275942772" Annex B1 Conditions of electricity power supply  PAGEREF _Toc275942772 \h 187  HYPERLINK \l "_Toc275942773" Annex B2 Biological safety cabinets  PAGEREF _Toc275942773 \h 189  HYPERLINK \l "_Toc275942774" Annex B3 Thimble connection  PAGEREF _Toc275942774 \h 196  Overview Over the past decade, tuberculosis (TB) has become a major public health problem globally, but particularly in Africa and Asia. This situation has been further complicated by the need to expand laboratory services to address the challenges posed by the epidemic of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS), and the emergence of multidrug-resistant and extensively drug-resistant TB. Many national TB control programmes (NTPs) have been in existence for nearly 30years, and directly observed treatment, short-course (DOTS) has been generally available during that time. DOTS is the basic five-point package that is the first component of the Stop TB Strategy. The five points of the package are: political commitment with adequate and sustained financing; early case detection and diagnosis through quality-assured bacteriology; standardized treatment with supervision and patient support; effective drug supply and management; and monitoring and evaluation of performance and impact. TB laboratory services were often neglected by NTPs, meaning that the DOTS strategy was less effective than it could have been. In the past few years, this situation has changed. Investment in laboratories has increased, due to increasing recognition of the role of the laboratory in TB control and in managing drug-resistant TB, and the launch of the Global Laboratory Initiative in 2007 by the Stop TB Partnership. Scope and content If TB laboratory services are to effectively support NTPs through TB diagnosis and monitoring, they need to provide reliable, valid and timely results. High-quality equipment and reliable supplies are essential for quality-assured laboratory services. However, governments, NTPs and donors responsible for the procurement and management of TB laboratory equipment and other commodities lack up-to-date, standard, international guidance. Consequently, there are many examples of wastage of resources; for example, purchase of large quantities of materials that are: inadequate (e.g. centrifuges not adapted for the recovery of TB bacilli, monocular instead of binocular microscopes); of poor quality (e.g. cheap microscopes that break within months); or inappropriate (e.g. some types of biological safety cabinets). This document has been developed to fill this gap by providing current, standard guidance on procuring TB laboratory equipment, consumables and supplies. The content is based on current international practice in commodity management. Purpose and objectives The purpose of this document is to provide guidance to governments, NTPs, donors and others responsible for the procurement, logistics and management of laboratory equipment and other commodities used in TB laboratory services. Such services include reliable TB microscopy and culture, and drug susceptibility testing. The document will be useful in developing systems for the efficient and timely procurement of quality laboratory equipment and supplies, and the ongoing management of all TB-related laboratory commodities. The objectives of the guidelines are to: strengthen and build capacity of TB diagnostic services in TB laboratory commodity management systems; and ensure that high-quality TB diagnostic commodities are provided and available at all times. Each countrys ministry of health should ensure that these guidelines are followed in setting standards, quality assurance mechanisms, reporting mechanisms, and monitoring and evaluation systems. Structure of document The document is divided into two parts: PartA provides guidelines for effective management of TB laboratory commodities it covers procurement mechanisms, logistics, quantification and management systems. PartB details the technical specifications for equipment, consumables and reagents needed to perform all techniques for the diagnosis of TB it covers microscopy, culture, identification and drug susceptibility testing of Mycobacterium tuberculosis using conventional methods and molecular biology. Part B also provides additional details on the specifications and requirements for sensitive equipment such as biological safety cabinets; this information will help to improve biosafety and guarantee infection control in TB laboratories. Part A Guidelines for countries on procuring and managing laboratory equipment, supplies and other commodities A1 Introduction Effective care and treatment of tuberculosis (TB) requires an effective national TB control programme (NTP). In turn, NTPs require support from fully functioning laboratory services that provide reliable, valid and timely results. Services need to include the diagnosis of TB infections and the monitoring of treatment at every level of care. To be fully functional, a laboratory service requires that laboratory commodities are available as needed. These commodities include equipment and supplies, such as laboratory reagents, diagnostic kits and other consumables. In contrast to the management of pharmaceutical commodities, little attention has been given to the management of commodities in laboratories. A lack of overall laboratory management often leads to problems at facility level. Thus, NTPs need to concentrate on those aspects of equipment and supply-chain management that are required for a fully functional TB diagnostic and monitoring service. This approach requires effective collaboration between laboratory staff and those making decisions about the TB commodity management system. In terms of TB commodity management, the role of the national TB laboratory is to set standards; provide quality assurance and reporting mechanisms; and evaluate the quality, accuracy and performance of the equipment and supplies. More specifically, the national TB laboratory, in collaboration with the NTP, should be responsible for: selecting equipment and supplies, and setting specifications and quantities; participating in the budgeting and planning process this includes being involved in verification of tender bids and awards of contracts for TB laboratory equipment and supplies; working with local and national procurement committees for purchasing equipment and supplies; and arranging for laboratory managers and staff to be trained in equipment and commodity management. This part of the document covers systems for management of laboratory commodities (ChapterA2), management of equipment (ChapterA3) and monitoring and evaluation (ChapterA4). It also provides annexes that contain the two workbooks for commodity management (AnnexesA1 and A2), examples of inventory control forms (AnnexA3) and a list of resources for further reading (AnnexA4). A2 Laboratory commodity management systems A2.1 What is commodity management? Laboratory commodity management involves many different activities that must be carefully planned and coordinated. The aim is to ensure that the right commodities, of acceptable quality, get to the right place at the right time to perform laboratory tests for patient diagnosis, care and management. Managing laboratory commodities, in any setting (public or private sector) and at any level (local, regional, provincial or national), follows a well-recognized cycle of selection, procurement, distribution and use, as shown in FigureA2.1. These activities are carried out in health systems that provide the necessary infrastructure and organization, financial and human resources, and information systems. Effective and efficient performance of these systems requires a framework of policy, law and regulation that upholds a commitment to the availability of commodities and their appropriate use.  Figure A2.1 Commodity management cycle Adapted from: Management Sciences for Health in collaboration with the World Health Organization. Managing Drug Supply, 2nd ed. (revised and expanded). West Hartford, CN, Kumarian Press, 1997. A2.1.1 Components of the commodity management cycle The components of the commodity management cycle shown in FigureA2.1 are defined below: Selection the process of establishing a list of laboratory equipment and commodities; usually decided at national policy-making level. The agreed list is then used to plan and decide which types of laboratory equipment and commodities to purchase. Procurement the process of acquiring (quantifying, ordering and purchasing) laboratory equipment and supplies from private or public suppliers through purchases from manufacturers, distributors or agencies, or through donations. Procurement includes: quantification the process of estimating quantities of a specific item needed for procurement for a specific period of time, and the financial requirements needed to purchase those items; and forecasting the process of projecting future needs over a longer period of time. Distribution the process of transporting, delivering and receiving laboratory supplies; appropriate storage; inventory control; and information systems for receipt and disbursement. Use the safe and effective use of TB laboratory diagnostic services and commodities; includes performing tests effectively and efficiently, and technical aspects of testing. Management support and preparedness essential to all the functions listed above. Management of the procurement and distribution parts of the cycle is particularly important; for example, proper quantification is vital to ensuring that adequate stock levels of TB laboratory commodities are always available. The laboratory diagnostic system depends on: organization of the system infrastructure and equipment; financial management effective integration of planning, finances and budgeting; information management systems maintenance of accurate, useful, and up-to-date information systems, proper documentation and record keeping; human resource management identification and motivation of adequately trained and capable staff; and monitoring and evaluation includes quality assurance and supervision. Policy, law and regulation defines the goals and parameters for effective management of the laboratory services. Each of the above components must be carried out within the policies and legal framework of the country. All of the above components operate within the local political, social, cultural and economic context. This influences the nature of the activities, but not the nature of the relationships. The following section deals with the practical aspects of the cycle of selection, procurement, distribution and use of all laboratory commodities. A2.2 Selection Careful planning for and selection of essential TB laboratory commodities are critical. For example, it is important to be selective in the range and type of TB diagnostic commodities limiting the list can lead to better supply, more knowledge and experience (through buying reagents that staff are familiar with), more rational use and lower costs. Selection is one of the most cost effective areas of intervention, because it has both clinical and economic implications. In terms of diagnosis, selection of commodities has implications for procurement, local production, training and ordering of tests. Policy makers need to understand the reasoning behind the selection, and this reasoning should be enshrined in the national TB policy and in the procedures followed by the selecting committee. The recommendations of the World Health Organization (WHO) Prequalification of Diagnostics Programme should be followed; this will help to ensure that laboratories purchase affordable diagnostic commodities of assured quality that are appropriate for use in resource-limited settings. To ensure adequate availability of essential laboratory commodities, health managers and policy makers must seek input from users (clinicians and laboratory staff) and review current information on: TB in the country; disease episodes; national health policies and plans; budgetary constraints; and product availability (inventory and consumption) this information is gathered from the procurement, distribution and use processes. A2.2.1 Selection of reagents and supplies Laboratory staff should be involved in decisions on selecting reagents and supplies. The selection process involves: reviewing the health problems or conditions to be prevented, tested for and treated at health facilities; developing a list of reagents and supplies by level of health care, using agreed criteria; choosing appropriate packaging or unit sizes; and basing the selection process on relevance, proven efficacy and safety, performance in a variety of settings, quality, costbenefit ratio, previous experience, location of manufacturer and capacity of laboratory staff. A2.2.2 Selection of equipment Laboratory staff should be involved in decisions on selecting capital equipment. Selections should be made on the basis of performance, local needs, cost, value for money and the availability of servicing, spare parts, reagents and consumables needed to run the equipment. In addition, equipment should be selected based on: policy guidelines; broad consultation with both users and maintenance staff (equipment must be user friendly); bidders having sufficient knowledge of maintenance, rather than being mere traders; and training for maintenance and training of users being a condition of the contract or purchasing agreement. Part B of this document has further information relating to selection of laboratory equipment and commodities for a country. It provides lists of equipment, consumables and chemicals needed for the different laboratory activities. It also has detailed specifications for selected items. Countries can use the lists in PartB to: prepare a standard list of equipment and commodities that meets local needs; select the items needed for the country at each level of health care; and select items for procurement or donations. A2.3 Procurement The effectiveness of the procurement system determines the availability and cost of essential laboratory commodities. Good procurement practices depend on reliable and accurate quantification of needs, transparency in selection of suppliers and in management of bids or contracting, and quality assurance of commodities. The procurement cycle for TB laboratory commodities shown in FigureA2.2 is identical to that used for procurement of drugs and pharmaceuticals. It includes most of the decisions and actions that determine the quantities of commodities obtained, the prices paid, and the quality of the commodities received.  Figure A2.2 The procurement cycle for TB laboratory commodities An effective procurement process for commodities for TB laboratories should efficiently: procure the right commodities in the right quantities; obtain the lowest possible purchase price; ensure that all commodities procured meet recognized standards of quality; arrange timely delivery; set the purchase-schedule formulas for order quantities and safety stock in such a way as to achieve the lowest total cost at each level of the health-care system; train staff to have expertise in laboratory, managerial, economic and even political skills; have procurement procedures that are fair, transparent and sound, and accord with generally accepted international standards; use effective transport and communications facilities; have access to reliable information about inventories and consumption; have good storage facilities; and facilitate timely customs clearance. A2.3.1 Quantification Quantification helps: to avoid stock-outs and ensure continuous availability of essential supplies; to avoid wastage due to over-stocking; to make the best use of scarce resources and to budget within the laboratorys means; to facilitate central bulk purchasing by providing sufficient details from health facilities to allow central orders to be placed well in advance; to increase the effectiveness of an existing laboratory supply programme budget; in the preparation of, and justification for, a budget; in planning for new or expanding programmes and policies; in calculating emergency needs; in resupplying an existing supply network that has become depleted of laboratory supplies; and in estimating how much refrigerated space might be needed in the future. TableA2.1 lists symptoms of good and poor quantification. TableA2.1 Symptoms of good and poor quantification Symptoms of good quantification Symptoms of poor quantificationConsistent availability of supplies Low wastage No over- or under-stocking Service providers have adequate TB diagnostic reagents and supplies Cost effective Rational adjustment to budgetary constraints Rational use of reagents and supplies Easy management of stock Fulfilment of demand Satisfied clientsShortages and stock-outs Surpluses and expired stock Breaks or delays in services, or both Loss of coverage clients or patients who come for TB testing are turned away Lack of treatment clients or patients who are sick and need TB treatment are not diagnosed or do not start treatment Inappropriate treatment Inaccurate budget estimates Suppression or distortion of demand Dissatisfied clients and loss of business; for example, doctors send their patients to a private clinicA2.3.2 Methods used in quantification In relation to TB laboratory consumables, there are several primary methods of quantifying how much to order: consumption, adjusted consumption and morbidity. Each method is discussed below. Consumption method The consumption method so called because it is based on how much of a product is used (or consumed) by the system employs historical data on past use of commodities to calculate the quantities that will be used in the future. The data required should be readily available in both the central store and individual laboratories. If this is not the case, then a laboratory information management system needs to be put in place to collect the required data. The consumption method assumes that current usage patterns will continue. To be of value, the consumption data on which projections are based must be accurate and reliable. The data must come from good inventory records, and from a system with a relatively uninterrupted supply and a full supply pipeline. If the supply is not continuous, the person doing the quantification may have difficulty interpreting actual past use; this will result in less reliable calculations and estimations. The consumption method can only be applied to products that have been used in the past, or to the direct replacement of a product that has been used in the past (e.g.one test kit being replaced by an equivalent). It is not useful for new services, where data on previous use will not be available. Adjusted consumption method The adjusted consumption method can be employed in situations where historical data on which to base orders are not available. In this method, data from another, similar laboratory service with a similar workload and numbers of clients or patients is used as the basis of the quantification. Morbidity method The morbidity method is related to disease patterns. It is useful when reliable laboratory data on workload and consumption are not available. A modification of the morbidity method is useful for calculating the quantity of laboratory supplies required for TB microscopy. This helps NTPs to accurately forecast the requirements for laboratory consumables, based on simple TB case data already being collected by the programme. AnnexesA1 and A2 contain tools to assist countries in the quantification of TB laboratory consumables and supplies. Detailed instructions on the use of these tools are provided. Selecting the best method for quantification of TB laboratory supplies in a country Option A If the country is already using a well-established supply-chain management system for laboratory supplies, and all TB laboratory requirements are procured satisfactorily, the country should continue to use this system. There is no need to set up a new, separate system solely for TB laboratory requirements. Option B If the country does not yet have an established, satisfactory supply-chain system, if there are problems with stock-outs and expired supplies, or if there is a lack of understanding of how to calculate appropriate quantities of items for techniques such as TB microscopy or TB culture and drug-susceptibility testing (DST), then the tools in AnnexesA1 and A2 should be used: Annex A1 contains the AFB-SuppliesProcurement workbook. This workbook is intended for use in quantifying and managing the consumables needed in laboratories doing acid-fast bacilli (AFB) microscopy at intermediate or national level. It is not intended for use in calculating supplies for individual laboratories. Annex A2 contains the CU&DST-Supplies workbook. This workbook is intended for use in quantifying and managing the consumables used in laboratories undertaking TB single culture and DST, and eventually in laboratories undertaking molecular tests. A2.4 Distribution Distribution is the process of transferring products from the source of supply to the place of consumption. It needs to be done economically and efficiently, and can be thought of as the art of getting the right amounts of commodities to the right places at the right times. In terms of TB commodities, distribution involves: moving commodities from the supplier through clearing ports; receipt; inspection; inventory control; storage (i.e.national or central medical stores); requisitioning of supplies; delivery to hospitals or districts; dispensing to end users; and reporting on use. Distribution systems are important. If they function well and are supported by good procurement practices, it is more likely that laboratory diagnostic services and point-of-care testing of assured quality will be available where they are supposed to be provided and when they are needed. A distribution system ensures continuous flow of supplies from a central point to the end-user facilities. It comprises the following elements: the systems design for example, the degree of centralization, push versus pull ordering, geographic or population coverage and number of different levels; an information system for example, inventory control, records and forms, consumption reports and information flow; appropriate storage for example, locations, building design, materials-handling systems and order-picking systems (e.g. first expired, first out [FEFO]); and delivery for example, collection versus delivery, choice of transport, vehicle procurement, vehicle maintenance, routing and scheduling of deliveries. FigureA2.3 illustrates theTB laboratory commodity distribution cycle.  FigureA2.3 TB laboratory commodity distribution cycle An effective distribution system relies on good system design and management. Such a system should: maintain a constant supply of TB diagnostic commodities; keep TB diagnostic commodities in good condition throughout the distribution process; include visual inspection of reagents and supplies, and testing of quality by laboratory staff; minimize losses of TB diagnostic commodities due to spoilage and expiry; maintain accurate inventory records to prevent stock-outs or over-stocking, while at the same time maintaining enough inventory to sustain good service levels at all of the systems distribution points; maintain a register of TB diagnostic commodity needs, to prevent future stock-outs and to analyse consumption data; locate storage warehouses in a way that optimizes the infrastructure available for storing stock when needed, and facilitates distribution to peripheral health facilities; use available transportation resources as efficiently as possible; reduce theft and fraud; and provide information for planning TB diagnostic commodity needs. Procurement, port clearing, receipt and inspection can be done by the national laboratory or the central stores, depending on the countrys policy and the availability of staff. A2.5 Store management Good management of storage and inventory involves monitoring expiration dates, inventory levels, unexplained losses (leakage) and storage conditions such as light, temperature and sanitation, which are particularly critical for diagnostic reagents. The role of the central store is to receive, hold and distribute all TB laboratory products for the public sector. The process should be controlled by an inventory control system, supported by an information system for performance monitoring; both of these are described below. Definitions The inventory is the total of all products kept on hand at any storage point. Inventory control is the function of supply management, which focuses on providing sufficient stocks of laboratory commodities while minimizing handling costs. Inventory control includes requisitioning and issuing products, financial accounting, and preparing the consumption and stock balance reports necessary for procurement. A2.5.1 Inventory control systems The purpose of an inventory control system is to manage procurement and stock movements. Which commodities should be stocked? Based on the selection criteria discussed above, the commodities stocked (for the public sector) should be: listed in each countrys essential list of laboratory supplies and equipment; of value to the public health system; consumed on a regular basis; and of known frequency and lead time for ordering and purchasing. PartB of this document provides a comprehensive list of TB commodities. How much should be stocked? In TB control, stock-outs of diagnostic laboratory commodities are unacceptable. All TB diagnostic laboratory commodities are essential for diagnosis and follow-up tests of TB patients; therefore, they must be available at all times. A2.5.2 Information and records What is required for proper inventory control? Several computerized or manual (i.e.paper) records are required for effective inventory control. This document provides examples of the following basic records: stock card (also known as a bin card); monthly consumption record; inventory control form; and list of expired laboratory supplies form. These records are shown in AnnexA3. However, where local, national, regional, or facility record forms or cards are already in existence, these should be used instead. A2.6 Storage and safe disposal Definition Shelf-life is the length of time a product may be stored under ideal conditions without affecting the usability, safety, purity or potency of the item. A2.6.1 Storage conditions The following conditions need to be considered when storing laboratory supplies: restricting access to the laboratory and storage area to authorized personnel; cleanliness of benches and shelves, and appropriate methods for disposal of waste; humidity; temperature general and special requirements, and temperature monitoring; air-conditioning; adequate refrigerated space maintained at optimal temperatures (+2 to +6C); adequate freezer space (at (20C and (70C); emergency power supply; lighting (note: supplies stored in direct sunlight may deteriorate); and shelving and cupboards these need to be adequate, accessible and stable. A2.6.2 Organization of stock The following issues need to be considered when organizing stock: stock rotation by FEFO; first in, first out (FIFO); and expiry dates; use of stock or bin cards; and security for dangerous or expensive items. A2.6.3 Special precautions Special precautions need to be considered for: flammables, irritants and corrosives; fire risks (fire extinguisher); and pest control. A2.6.4 Safe disposal The safe disposal of laboratory waste and contaminated materials is of prime importance. These items represent hazards to both laboratory staff and the community. Also, the uncontrolled dumping of solid, liquid, chemical and biological laboratory waste can threaten the environment. A2.6.5 Types of waste Laboratory-generated waste includes: sharps; chemical waste expired reagents and consumables; human anatomical waste; blood and body fluids; solid waste such as cotton wool, tissue paper, culture plates with used media, used blood-giving sets, empty blood packs, used test-tubes and used glass slides; laboratory specimens; and equipment effluent. A2.6.6 Methods of safe disposal Waste can be disposed of by incineration or burial, both of which are discussed below. The method used will depend on the nature of the waste. Incineration Incineration provides high temperatures and destroys microorganisms; thus, it is the best method for disposal of contaminated wastes. Incineration also reduces the bulk size of wastes to be buried. Simple incinerators can be built from locally available materials such as bricks, concrete blocks, and used fuel or oil drums. Burial If incineration is not possible, all contaminated wastes must be buried in a rubbish pit and covered with soil to prevent the waste materials from being scattered. Rubbish pits should be at least 45m deep and 12m wide, and protected with a cover or fence to prevent scavenging. The rubbish should also regularly be covered with soil to prevent scavenging or incidental contamination of the environment. A2.6.7 Transport of waste material for incineration All materials for incineration must be documented in a logbook, which must include at least the following information: name of the porter and signature; supervisors name and signature; date of collection; source of the waste; weight of the waste; and signature of receiving officer at the incinerator. The porters supervisor must make sure that the material for incineration is properly packaged, that it is labelled as BIOHAZARDOUS material, and that the package is leak proof. The porter must wear personal protective equipment as necessary, and must transport the material as quickly as possible, using a route that avoids close encounters with members of the public, to protect them from exposure. A2.7 Rational use of TB laboratory commodities Current TB laboratory diagnostic services and commodities, as well as new diagnostics, should be used safely and effectively. This requires health workers (clinicians and diagnostic service providers), patients and the community to understand the role and functions of the laboratory in the control of TB. Health workers must know: when and who to test for TB; how to use test results in decision-making; and what findings should be reported and whom they should be reported to. Laboratory managers and staff must know: which tests to use; how to perform the tests; how to safely dispose of testing commodities and specimens after their use; what findings should be reported and whom they should be reported to; and how to maintain essential equipment and commodities. The community needs to know: the benefits of testing; and where the services can be obtained. Ensuring safe and effective use of diagnostic services and commodities will entail a range of interventions, including incorporation of laboratory testing into diagnostic and treatment guidelines, development and implementation of standard operating procedures, and use of job aids and other appropriate behaviour-change interventions targeted at clinicians, diagnostic service providers and care seekers in the community. The Tuberculosis Control Assistance Program (TBCAP) toolbox contains several tools that should be used in conjunction with this document to promote rational use of laboratory commodities. These include: TB laboratory standard operating procedures (SOPs); a management information system that includes a set of recording and reporting formats; microscopy external quality assessment a training package aimed at strengthening quality assurance of AFB microscopy; culture and DST a training package on biosafety for laboratories; and a country roadmap a generic document for strengthening laboratories. A2.7.1 Training of laboratory managers and staff To make proper use of this document and the TBCAP toolbox, laboratory staff at all levels of care will require adequate training. The training should focus on implementation of laboratory commodity management systems. A3 Equipment management ChapterA2 provided information on overall commodity management. This chapter covers some additional considerations required for managing laboratory equipment. A3.1 National equipment policy Countries should develop a laboratory equipment policy that includes all relevant activities. The policy should include an equipment maintenance plan, a budget for equipment maintenance (i.e. for repairs and spare parts) and guidance on equipment donations. A3.2 Criteria for selecting equipment When selecting equipment for purchase, laboratories need to take into account: the proposed use of the equipment; how well the equipment accords with the service provided; performance characteristics; facility and infrastructural requirements; cost; availability of reagents and consumables, and arrangements for supply of these materials; ease of operation; warranty; availability of technical support from the manufacturer; service contracts; location in the laboratory; available space and accessibility; and safety. Part B of this document provides detailed equipment specifications that will be useful when selecting equipment. A3.3 Acquisition Equipment may be acquired through direct purchase, lease or rental. It is often best to procure items of equipment centrally. If many of the same items are required, then bulk procurement may be the most cost-effective and practical approach. Similar considerations apply when acquiring equipment from donors. Regardless of how the equipment is acquired, laboratories must take into consideration: responsibilities of the manufacturer or distributor; conditions of the commercial sales contract; and customer-support plan and maintenance contracts. Each of these aspects is discussed below. A3.3.1 Responsibilities of the manufacturer or distributor The manufacturer or distributor must guarantee: provision of all reagents, consumables and culture materials at an affordable and sustainable price (note: distributors usually offer reduced prices for high consumption, so bulk or central ordering may be a distinct advantage); a reasonably lengthy expiration date on all reagents and consumables; acceptable shipment conditions and assistance with customs logistics, to avoid damage to equipment or deterioration of the reagents; installation of the equipment, staff training and ongoing technical support; provision of a parts manual and an operators manual; a trial period for the equipment, after which it can be returned if it is not deemed suitable; and ongoing maintenance and repairs, including emergency services. A3.3.2 Conditions of the commercial sales contract The sales contract must be reviewed carefully before completing the purchase. The contract should clearly stipulate all of the above responsibilities of the manufacturer or distributor. A3.3.3 Customer-support plan and maintenance contracts A customer-support plan and maintenance contracts should be available for all items of capital equipment. Maintenance contracts are essential for all automated equipment (e.g.Mycobacterium growth indicator tube) and biosafety equipment. Laboratories should pay particular attention to contracts for biological safety cabinets these should provide for regular maintenance. A3.4 Installation Before installation: verify that physical requirements have been met; these include electrical, space, ventilation, water supply and ambient temperature requirements, and safety checks; and confirm who is responsible for installation. Upon receipt: verify the package contents; do not attempt to use the equipment before it has been properly installed; and generally, in the case of capital equipment, ensure that the equipment is installed by the manufacturer. After installation: establish an inventory record for the equipment; define the conditions for use; develop and implement protocols for calibration, performance verification and operating procedures; establish a maintenance programme; and provide training for all operators. A3.5 Validation and calibration Validate the performance of new equipment and calibrate it before use by: testing known samples and analysing the data; establishing stability or uniformity in temperature-controlled equipment; checking the accuracy or precision of pipettes; and checking the speed (in revolutions per minute [rpm]) of a centrifuge. A3.6 Maintenance and troubleshooting Maintenance involves systematic and routine cleaning, and adjustment or replacement of instrument and equipment parts. It should be performed regularly either daily, weekly or monthly, depending on the equipment. Examples of maintenance are cleaning optical lenses, adjusting thermostats and changing motor brushes. If a piece of equipment malfunctions, users should: check the manufacturers instructions; determine the source of the problem for example, the sample, the reagent, the equipment, the electrical supply or the water supply; and make one change at a time, to attempt to diagnose the source of the problem. A3.7 Professional service and repair In terms of professional service and repair, laboratory managers should schedule regular servicing, by the manufacturer or representative, for all key items of equipment. To ensure cost effectiveness, it is preferable that all items of the same model (e.g. microscopes) are serviced at the same time. Basic items of equipment such as water-baths may be maintained by local biomedical service technicians. A3.8 Retiring equipment The questions to ask in terms of retiring equipment are: When should an item be retired? This might occur when experts indicate that the item cannot be repaired, or is outmoded and should be replaced with a new model. Why should an item be retired? Reasons might be to avoid issuing inaccurate test results, to free up valuable space and to reduce hazards. How should an item be retired? A useful approach is to salvage any usable parts, taking into account any biohazards, and then follow safety disposal procedures for any parts that cannot be reused. A3.9 Equipment maintenance programmes The benefits of a maintenance programme are: safety of equipment; fewer interruptions of work; lower repair costs; equipment lasting longer, rather than needing to be replaced prematurely; less need for standby equipment; identification of high maintenance costs; reduction of variation in test results; and greater confidence in the reliability of results. A good equipment maintenance programme: helps the laboratory to achieve a high level of performance; lengthens instrument life; reduces interruption of services due to breakdowns and failures; improves customer satisfaction; and improves the confidence and knowledge of laboratory technologists. A3.10 Equipment management oversight In terms of oversight of equipment management, laboratories should: assign responsibilities for all activities; train all personnel on equipment management requirements and responsibilities; and monitor equipment management activities by: routinely reviewing all records; ensuring that all procedures are followed; and updating procedures, if necessary. A3.11 Donations of laboratory equipment and other commodities Countries and laboratories need to ensure that donations of commodities to TB laboratories are handled properly. This means making sure that: donations are based on need expressed by the recipient country, ministry of health or NTP; all donated capital equipment and TB diagnostic commodities comply with the quality standards of both the donor and the recipient country; on arrival, all donated TB diagnostic commodities have a remaining shelf-life of at least one year; all TB laboratory equipment and diagnostic commodities are labelled and have instructions in English or in a countrys national official language that is easily understood by health professionals; recipients are made aware of all donations of TB diagnostic equipment and commodities that are being considered or are under way; the declared value of the TB diagnostic equipment and commodity donation is based on the wholesale price of its equivalent in the donating country; wherever possible, costs of international and local transport, warehousing and port clearance are paid by the donor agency; and wherever possible, a maintenance contract and adequate supplies of consumables accompany donations of capital equipment. A4 Monitoring and evaluation The entire laboratory diagnostic system depends on effective integration and management of finances and budgets; maintenance of accurate, useful, and up-to-date information systems; identification and motivation of capable staff; and the institution of monitoring and evaluation systems. The expertise and organizational framework provided through management support are critical in each process of laboratory services and technology management. A4.1 Monitoring A commodity monitoring system provides information that the NTP and the national laboratory can use periodically to determine whether the commodity management system is performing as expected. For example, if all the TB laboratory commodities ordered are being delivered and recorded, the order quantities are right and no stock-out or over-stock situation arises, then the monitoring will find that the book record and the physical count of the equipment and supplies correspond. Other uses of the monitoring system include: determining whether commodities are stored at the right temperature and that expiry dates of supplies are known in advance this allows supplies to be returned to the suppliers or redistributed to other centres before they become useless; assessing whether the samples collected are useful, equipment is calibrated routinely, the supply of reagents is adequate, and so on; and providing information that can be used to assess the workload of the laboratory staff. A4.2 Evaluation The purpose of evaluation is to assess the impact of intervention, usually in the long term. Short-term evaluation can also be undertaken, but will reflect only the outcome of an intervention, not the impact. A4.3 Indicators for monitoring and evaluation Examples of indicators for monitoring and evaluating laboratory commodity management include average percentage of: stock-out time for a set of tracer commodities at a TB microscopy site; unexpired tracer commodities at a TB microscopy site; and stock records that correspond with physical counts for TB laboratory tracer commodities. Such information is helpful in making timely and informed management decisions. In turn, such decisions help to ensure the continuity and quality of services. Annex A1 AFB-SuppliesProcurement workbook The Excel workbook AFB-SuppliesProcurement is intended for use in quantifying and managing the consumables needed in laboratories doing AFB microscopy at intermediate or national level. It is not intended for use in calculating supplies for individual laboratories. INSTRUCTIONS FOR USE Once essential information on stocks and performance has been entered and a few other parameters have been set in the appropriate sheets, the workbook will automatically show the quantities that are theoretically needed. The workbook can then be printed, to be used as the paper request. The supplying level can make further use of this tool by filling in quantities actually supplied (i.e. manually changing the quantities to what is feasible, taking into account stock limitations). The completed form can also be used as a delivery and receipt document. Another sheet uses the information entered, plus the information from a country-specific database, to calculate costs of estimated needs. Adding specifications will produce a sheet for procurement for example, at national level. There is also a hidden sheet that only performs calculations and shows the various intermediary steps. This sheet should remain hidden unless there is a need to make changes to the formulas or to check on the correctness of the calculations. All values already entered are only examples. It is the responsibility of the user to adapt these values according to the actual: parameters (smears performed, laboratories functional and stock situation); guidelines (stains formulation, procurement period); and other conditions or estimates (e.g. consumption) that are valid for the specific setting where the tool will be used. The formulas and links built into this workbook are complicated and should not be disturbed. Although the formulas and links have been protected, it is the responsibility of the head of the laboratory services to complete this protection by giving the hidden sheet a password and keeping that password confidential. (Failure to protect this sheet will lead to problems at some point.) Similarly, a blank master copy of the entire workbook should always be kept in reserve. This original should be copied to a safe directory, where it should be saved with an extension indicating the name of the laboratory; for example, AFB-SuppliesProcurementTanzania. Sheets within the workbook ItemsDatabase sheet The ItemsDatabase sheet is the general database from which a country-specific database should be constituted at national level. It contains a variety of small materials and supplies (i.e.consumables), and lists their specifications, prices and so on. CountryItems sheet The CountryItems sheet is an example of a country-customised database of items needed for AFB-microscopy work. It can be further adapted by being copied and pasted from the general database with correction of packing sizes, prices and so on, as needed. If there are unnecessary items (e.g. names, specifications and prices), it is best to replace these without deleting lines because the item names are automatically copied to other sheets (e.g.stock ledgers and projected needs), where they may be linked to factors for calculation. Never attempt to sort the CountryItems sheet because this will corrupt the calculations in other sheets. VariableData sheet The VariableData sheet contains information needed for the calculations and must be updated by users. Information that must be entered includes: numbers of smears performed earlier over a specified number of months (ZiehlNeelsen [ZN] smears versus auramine smears) these should be placed in boxes at the top of the sheet; number of functional microscopy laboratories to supply these should be placed in boxes at the top of the sheet; quantities left in stock (report only those remaining at the requesting [intermediary] level) these should be placed in the second column; and quantities ordered previously and expected to arrive these should be placed in the third column. Make sure the counting and calculation units specified are respected for each item when filling in these data. Please also note that the quantities left in stock to be reported are only those remaining at the requesting (intermediary) level. Do not include stocks present at the laboratories at lower levels of the service. Dealing with items that are not needed Some of the sheets contain items that may not be needed. For example, it will generally be necessary to choose between sulfuric and hydrochloric acid. This can be dealt with: in the VariableData sheet by hiding the lines that are not needed (i.e. select the line; right click on the mouse and select Hide; to unhide, select the line, right click on the mouse and select Unhide); and in the FixedData sheet by setting these items to zero for the consumption factors. Do not delete lines from the sheets because this may disturb some calculations. The entire workbook should remain protected to make it impossible to delete lines. Adding items It may also be necessary to add items to the database. Lines that can be used to add items for microscopy are : line 20 for fluorescence staining; line 21 for ZN staining reagent; and lines 48 and 49 at the bottom of the VariableData sheet, for items calculated by functional laboratory rather than by smears done. The details (name, unit) for these products need to be specified in the CountryItems sheet, from where they will be copied to this sheet. Calculation of quantities After the required information has been entered into the VariableData sheet, the quantities needed will appear automatically, provided that essential information has also been entered in the FixedData sheet. The quantities combine consumption (period specified for consumption plus lead time period) and reserve stocks. They are expressed as units and also as packing units for clarity and versatility, appearing in the centre of the sheet. The columns to the right are intended to be used at the supplying (i.e.higher) level and when supplies are received, as explained above. Stocks The VariableData sheet contains a column titled Quantities ordered and expected to arrive, which is next to a column titled No. of months consumption in stock + coming. The latter column shows how many months the current stock of that item will allow the laboratory to continue to undertake AFB microscopy for the same values of the parameters (i.e.numbers of slides examined and numbers of functional laboratories), as entered. Low stocks will be flagged by a colour code that can be changed (colour and/or criteria) via the Format menu (Conditional Formatting). The default colour settings for number of months left are: orange, if the quantity left in stock is not sufficient to cover the period of consumption plus lead time specified; or red, indicating the need for an emergency supply, if the quantity left in stock has dropped below that required for the usual lead time, even without taking into account the consumption period. FixedData sheet The FixedData sheet only needs to be updated occasionally. At the top of the sheet are boxes indicating the number of months for which the consumption and reserve stock should be calculated, and the lead time (in months) for procurement (i.e.the time that usually elapses between ordering and availability of supplies). The left-hand side of the sheet has columns showing: the formulation of stains, expressed as grams or millilitres needed per litre of ready-for-use staining solution, following the national guidelines (central column); and the quantities per packing unit as supplied by the manufacturer (if unknown, this can be left open, in which case only the counting units of this item will be calculated). When the workbook is first used, these factors will need to be checked and adapted as appropriate; a similar approach will be needed to that given above for items not yet included or specified in the first sheet. The FixedData sheet contains only one line for methylene blue, which may be used as counterstain for both ZN and fluorescence microscopy. Should the concentrations of these solutions differ, then only the highest concentration should be added to the sheet. This will lead to more of the solution being ordered than is absolutely necessary, if the quantities left in stock are subsequently entered when estimating needs. The right-hand side of the sheet has two boxes for consumption factors that must be estimated: The upper box is used to record information on the consumption of ready-for-use staining solutions and other items expressed per smear. The lower box contains similar information to the upper box, but covers items for which the consumption is estimated based on number of functional laboratories rather than number of smears, expressing the consumption of these items per laboratory and per year. The formulas in this sheet are protected, but the sheet is currently not password protected. Countries are encouraged to add a password after adapting this tool to their specific needs and before releasing it for wider use. Adding password protection should prevent formulas being accidentally erased, which is usually followed by attempts at repair that introduce further errors. ToBeProcured sheet The ToBeProcured sheet takes the final calculations from the VariableData sheet, and adds the specification, pack size and price from the CountryItems sheet. The names have to be identical, which will be the case provided that the formulas have been left intact. The ToBeProcured sheet also shows the total cost of the order. The user can then enter the amounts to be procured in the column titled Number of packing units to order, which is surrounded by a heavy black line as a rounded number of packing units, and can correct the figures based on past experience, as necessary. The total cost of this final order will appear at the top of this box, which makes it possible to adjust quantities to work within the budget available. The Number of packing units to order column also allows the user to enter a zero amount for unused items or variations. Finally, any items that are not needed (zero, or no value entered) can be left out of the order by using the filter arrow in the Number of packing units to order column. Users should choose Custom and then specify is greater than zero; this will leave only items to be included in the order. The sheet can then be modified further by hiding columns, to produce a table containing all the elements needed to start procurement. Returning to a view that includes all items simply requires all filters to be reset to All. A final document for printing or other manipulations can also be prepared from the ToBeProcured sheet, by copying the complete sheet to a new Excel file. To do this, select the whole sheet by placing the cursor in the left-hand upper corner of the sheet, where the line and column numbering meet, right click and select Copy, then open a new book, move the cursor to the same corner, right click and select Paste. This manipulation can be done without unprotecting the sheet. It is also possible to use the sheet in a more flexible way by copying the entire sheet as described above, but then selecting Paste Special rather than Paste, and then pasting only Values. This will remove the formulas, allowing all usual manipulations, including sorting, without introducing errors.  HYPERLINK "../_envoi%20Hilary%20Cadman/AFBsuppliesProcurement%20V02.xls" AFBsuppliesProcurement V02.xls [include hotlink or address where file can be downloaded on obtained]  HYPERLINK "AFBsuppliesProcurement%20V02.xls"  Annex A2 CU&DST-Supplies workbook The Excel workbook CU&DST-Supplies is intended for use in quantifying and managing the consumables used in laboratories undertaking TB culture and DST, and eventually in laboratories undertaking molecular tests. The CU&DST-Supplies workbook contains several sheets for input by users or for output, plus a laboratory-specific database of the items routinely used and a stock ledger for these items. There are also a number of hidden sheets that perform interim calculations and only need to be seen infrequently. Overall, the formulas and links built into this workbook are complicated and should not be disturbed. Although the formulas and links have been protected, it is the responsibility of the head of the laboratory services to complete this protection by giving the hidden sheet a password and keeping that password confidential. (Failure to protect this sheet will lead to problems at some point.) Similarly, a blank master copy of the entire workbook should always be kept in reserve. This original should be copied to a safe directory, where it should be saved with an extension indicating the name of the laboratory; for example, CU&DST-SuppliesDhakaNRL. This version can be used to customize items and parameters; it should be saved with the same name, to act as a master file for the laboratory. In each new year, laboratories should start by saving a copy of the customized version of the workbook, adding an extension for the year; for example, CU&DST-SuppliesDhakaNRL2009. This copy will then be the working file for the particular year. There are two ways in which estimates for procurement can be made using the workbook; these methods, which are explained below, can be used separately or in combination: Consumption-based method. This method, which is recommended, involves basing future needs on recorded past consumption. It is simple because it is done automatically by the workbook and requires the user to set only a few parameters. However, this method will only deliver meaningful and accurate estimates if the stock ledger has been maintained regularly and completely. Projected needs-based method. This method involves basing future needs on the number of tests to be done, the time frame and the number of technicians. It is more difficult than the consumption-based method because it requires the user to specify the consumption of most items per test. However, this method may regularly have to be used alongside the consumption-based estimate; for example, when an increase in numbers is expected because of an upcoming survey. This system of projected needs can also be used to estimate total requirements, and will be the only method available if stock ledgers have not been well kept. The workbook uses both methods simultaneously where parameters have been specified. To avoid a double order due to use of the two systems for procurement, the user must specify which method of estimate should be used: consumption-based only; or projected needs-based only; or both methods in combination, with extra projected needs on top of average past consumption. Besides choosing which system to use for procurement, the user also needs to customize the CU&DST-Supplies workbook in several ways. Customization is required for items routinely consumed, their specifications and prices; consumption factors; stocks at a specific moment; months consumption to be covered by the order; reserve allowed; and expected lead time until arrival of the order. The workbook contains a database of items based mainly on the globally recommended lists; users will need to adapt this database extensively. The same is true for consumption factors, stocks left and past consumption in the ledger page. All values already in the workbook are examples only. It is the responsibility of the user to adapt these values to the actual situation. Sheets within the workbook Menu sheet The Menu sheet shows the different parts of the workbook, and contains hyperlinks to the sheets intended to be used regularly. Users can click on the boxes containing text in red font to open the appropriate sheet. The other sheets in the workbook all contain the command Return to menu in the upper right-hand corner of the screen. Users can click on this link to return to the Menu sheet. ItemsDatabase sheet The ItemsDatabase sheet lists small materials and supplies considered to represent consumables; only those that need to be procured (i.e. that are not produced or bought locally) should be included. The list is divided into different parts, from top to bottom of the screen, by area of work or type of supply. The uppermost part, which is surrounded by a heavy red border, should not be changed because it contains complex links to calculations of chemicals required for various tests. The packing units and prices can be changed to reflect local variation, but no other items in this box should be changed unless the password protection is first disabled (an approach that is strongly discouraged). Any lines that are not needed can be hidden, both in this sheet and in the sheets that depend on the ItemsDatabase sheet hiding lines that are not required is much safer than deleting them. To hide a line, select it; right click on the mouse and select Hide; to unhide a line, select the line, right click on the mouse and select Unhide. For some culture media, the sheet shows both the composite-medium base powder and the different chemical components. The various chemicals that may be needed for different decontamination and identification methods are also listed. Again, any options that are not used in a particular laboratory should be hidden in both this sheet and dependent sheets. The part of the ItemsDatabase sheet directly below the section bordered in red contains all the remaining items; these can be changed as necessary to suit individual needs. However, the user should hide unnecessary items, or replace their names, specifications, prices and so on, rather than deleting lines. This approach is recommended because names are copied to other sheets (stock ledger, projected needs and so on), where they may be linked to factors for calculation that apply only to the particular item. It is best to add new items on the first empty line at the bottom of the sheet, because this line contains preset links to the other sheets. It is also possible to insert lines for new items by copying formulas downwards in all sheets; if this is done, the user must ensure that the inserted lines are placed identically (i.e. in-between the same lines) in all sheets, and that the formulas are copied downwards in the same way. Never attempt to sort the ItemsDatabase sheet because this will corrupt the calculations in some other sheets. The filter arrows that are given in the heading of many of the columns can be used to locate particular items quickly. Clicking on an arrow produces an alphabetically sorted list in a small window. Typing a character makes the cursor jump to the first item of the list starting with that character. Clicking to select an item causes only that item to be shown, with all others remaining hidden; the filter arrow is now displayed in blue to show that only a selection of the items in the column is being displayed. To revert to the complete database, the user clicks on the filter arrow and selects All at the top of the list (or types A). If more than one filter is preset for a particular sheet (i.e. more than one column has a filter arrow), it may be necessary to select All for other columns in order to see the complete database. StockLedger sheet In the StockLedger sheet, names of the items and units for counting (given in columns A and B) are filled automatically from the ItemsDatabase sheet. The columns to the right should be updated quarterly: At the start of a new year, users enter the total consumption for the previous year and the stock in hand. For items that are consumed only infrequently (e.g.some glassware), it may be better to use an average consumption over several previous years. Each quarter, users enter the amounts added to the stock, losses (i.e. items not consumed but stolen, expired, etc.) and the stock situation at the end of the quarter, using the columns inside the red border. The units for counting preset in the sheet must be respected. Consumption is calculated from these data rather than from current stock. Thus, actual quantities in stock must be obtained by physically counting items in the store each quarter; in the long run, this is far more accurate than obtaining the numbers by addition and subtraction. Once the data listed above have been entered, consumption during the quarter appears in the database. The months consumption left in stock also appears; this shows how many months the current stock of a particular item will last, using an average consumption over the previous 12months. Low stocks will be flagged by a colour code that can be changed (colour and/or criteria) via the Format menu (Conditional Formatting). The default colour settings for number of months left are: orange, if the quantity left in stock is not sufficient to cover the period of consumption plus lead time specified; or red, indicating the need for an emergency supply, if the quantity left in stock has dropped below that required for the usual lead time, even without taking into account the consumption period. The consumption calculation in this sheet is based on averages of past consumption, always taking into account the previous 12months, according to the state of completion of the ledger. The most recently filled stock positions are taken into account in the needs calculations. As for the ItemsDatabase sheet, the StockLedger sheet contains many items, many of which may not be needed and should therefore be hidden. If users wish to delete unused items, they should do so only in the ItemsDatabase sheet, which is the master for all sheets. For some chemicals that are rarely used and for the TB drugs needed for preparation of DST media, the system described above may not be practical. For example, it is not easy to determine the amounts left in stock if there is only one container or vial in use that will last for more than one year: For drug powders, it may be preferable to have a system whereby the items are replaced every two years, and supplies of those items are ordered for two years at a time, without updating their stock position in the way described above. For chemicals that are consumed at a rate of less than one packing unit per year, it may be preferable to count only complete, unopened packing units when determining the stock position, ensuring that at least one unit is always kept in stock. Alternatively, the weight of the container can be determined at receipt (i.e.weight of container plus contents, minus contents weight as indicated on the label), and noted on the stock card or ledger. When an inventory is taken, the containers in use would then be weighed with their contents, and the container weight subtracted, to determine the weight of the material remaining in the container; this weight would then be entered in the supplies workbook. ParameterEntry sheet The upper part of the ParameterEntry sheet should always be used to indicate whether the estimated needs should be based on: previous consumption (preferable, provided the ledger has been well maintained); or projected needs; or both previous consumption and projected needs (in situations where additional needs, above usual consumption, are expected). The selection is made by simply selecting the method of choice in the appropriate box in the upper part of the sheet. Selecting the method correctly avoids situations in which needs are erroneously estimated in duplicate. The upper part of the ParameterEntry sheet should also be used to specify: the months consumption targeted by the order; the reserve allowed; and the expected lead time for procurement (based on previous experience, but often more than 12months). The lower part of the ParameterEntry sheet may not need to be filled out if the estimation of needs is based on previous consumption. However, if the estimation is based on additional needs, the main details of techniques need to be specified: culture media used and their volumes, for culture, DST, identification and preservation of strains; and decontaminant and neutralization buffer used and their volumes, for decontamination of specimens. The expected numbers of these tests also need to be specified. In situations where this calculation complements consumption-based calculation, the numbers entered should only be quantities above or below those used in the previous year (note: to indicate quantities below those of the previous year, use a minus sign before the number). NeedsProjected(Extra) sheet The values in the NeedsProjected(Extra) sheet only have to be entered in cases where needs are estimated based on previous consumption. Values for culture media and other chemicals do not need to be specified because they are predefined in the Chemicals_for_MediaDecontIdent sheet. However, consumption of other items tubes, pipettes, gloves, etc. varies between laboratories, and needs to be set once, based on standard operating procedures. The list of items and their counting or calculation units, copied automatically from the ItemsDatabase sheet, appears in the first two columns. In the columns to the right, users enter values for consumption per test (various types of tests are specified on top), per month or per technician working with a test. Calculations are performed automatically in the hidden part of the sheet. NeedsConsumptionBased sheet The NeedsConsumptionBased sheet is a hidden sheet that contains calculations of expected needs based on past consumption, as shown in the stock ledger. The user is not required to give any input here, and the sheet should remain hidden. Chemicals_for_MediaDecontIdent sheet The Chemicals_for_MediaDecontIdent sheet is another hidden sheet that contains calculations of expected needs for chemicals used for preparation of media, decontamination, and the main biochemical identification tests paranitrobenzoic acid (PNB), thiophene-2-carboxylic acid hydrazide (TCH), niacin, catalase and nitratase. The total volumes of media and reagents needed are taken from the parameters specified in the ParameterEntry sheet. Values for each chemical are summed automatically to yield a single quantity, even though a chemical may be used in more than one medium or technique. For most media, two calculations will be made one applicable to use of a composite-medium base powder and one providing the amounts of the different chemical components and both options will appear. Also, the various products possibly needed for different decontamination and identification methods will appear. Where this sheet contains information on items that are not used, the best approach is to hide the lines for the unused options in the output sheets. The user is not required to give any input here, and the sheet should remain hidden. CombinedEstimates&Stock sheet The CombinedEstimates&Stock sheet combines two methods of needs calculation and the most recent stock position entered in the StockLedger sheet. The two columns on the left Item in consumption calculation sheet and Estimated needs based on consumption are again copied from the ItemsDatabase sheet, but the estimated needs and stock are obtained automatically from other sheets in the workbook through a lookup function. The automated function works even if items are not on the same line or in the same sequence, but it requires the names to be written in exactly the same way in different sheets. This causes a problem only in situations where the sheets have been changed by the user. If the automated function cannot find an item, or if the name is written differently, the text N/A will appear instead of the expected number. The CombinedEstimates&Stock sheet also contains columns bordered in red, which may need to be completed by the user; they are: a column for quantities left in stock this should only be completed in situations where the StockLedger sheet is not up to date (or is not used); and a column in which to enter quantities that have been ordered, are expected to come but have not yet arrived. The amounts calculated as needing to be procured appear on the right (an automated function having subtracted the stock left and quantities ordered and expected to arrive). The amounts calculated based on previous consumption, or those based on projected work, or both, may be used automatically, depending on what was specified as the basis for calculation in the ParameterEntry sheet. ToBeProcured sheet The ToBeProcured sheet takes the final calculations from the CombinedEstimates&Stock sheet and adds the specification, pack size and price by looking these up in the database (again, the names have to be exactly the same in each place; this will not cause a problem provided that the formulas have been left intact). This sheet also shows the total cost of the order. The user can then enter the amounts to be procured in the column bordered in red (titled Number of packing units to order), giving the amounts as a rounded number of packing units, and perhaps adjusting the values based on past experience. The total cost of this final order will also appear at the top of the sheet; this allows users to work within the budget available by modifying quantities, as necessary. The red-bordered column also makes it possible for users to set unused items or variations (e.g. media components versus complete-base powders) to zero. Finally, the items that are not needed (zero, or no value entered) can be omitted using the filter arrow in the red-bordered column. Choosing Custom and then specifying is greater than zero will leave only items to be included in the order. Users can further modify the sheet by hiding columns, to produce a table containing all elements needed to start procurement. Returning to a view that includes all items simply requires all filters to be reset to All. A final document for printing or other manipulations can also be prepared from the ToBeProcured sheet, by copying the complete sheet to a new Excel file. To do this, select the whole sheet by placing the cursor in the left-hand upper corner of the sheet, where the line and column numbering meet, right click and select Copy, then open a new book, move the cursor to the same corner, right click and select Paste. This manipulation can be done without unprotecting the sheet. It is also possible to use the sheet in a more flexible way by copying the entire sheet as described above, but then selecting Paste Special rather than Paste, and then pasting only Values. This will remove the formulas, allowing all usual manipulations, including sorting, without introducing errors.  HYPERLINK "../_envoi%20Hilary%20Cadman/CU&DSTsupplies.xls" CU&DSTsupplies.xls [include hotlink or address where file can be downloaded on obtained] Annex A3 Examples of inventory control forms Stock or bin card Stock (or bin) cards keep track of stock movements within a store or health facility. A stock card is the basic record needed to establish accurate quantities of individual items to reorder. A separate stock card is needed for each inventory item. Basic components of a stock card A stock card should contain the following components: Full name and chemical formula. Enter the full name (for all items) and the chemical formula (as appropriate) of the commodity; for example: Unigold HIV rapid test kit potassium iodide [KI] sulfuric acid [H2SO4]. Unit. Provide the distribution unit of measure. Determine in what unit the item will be distributed (e.g. by packs of 100 tests or by base units such as grams or litres). When supplies arrive and are not in predetermined units of measure, employees in the storeroom can become confused when attempting to fill out stock cards. Item code. Record the unique identification code used by the health system. This code is normally found in the catalogue of the medical stores. Expiry date. Find the expiry date printed on the container. This date is determined by the manufacturing company. When the item passes this date, the manufacturer does not guarantee the potency, purity or safety of the product. Minimum stock level. Determine and record the minimum stock level. When stocks are depleted to the minimum stock level, the item must be reordered. Maximum stock level. The maximum stock level is the total quantity necessary to meet the needs of the health facility for a specific period. How to use the stock card Enter the opening balance and the date the stock is checked. Receiving stock. Enter the date stock is received, the quantity received according to the issue unit, and the stock balance (in this case, the stock balance will equal the previous balance plus the quantity received). Issuing stock. Enter the date stock is issued, the quantity issued according to the issue unit, and the stock balance (in this case, the stock balance will equal the previous balance minus the quantity issued). Tracking loans. Enter the date stock is borrowed and the location the goods are loaned to or borrowed from. Enter the quantity issued or received according to the issue unit, and record the stock balance (in this case, the stock balance will equal the previous balance minus the quantity loaned or plus the quantity borrowed). Figures AA3.1 and AA3.2 show the format of a typical stock card and an example of a stock card for a laboratory storeroom. FigureAA3.1 Typical stock card Name and strengthUnitItem codeExpiration dateAverage monthly consumptionMinimum levelMaximum levelRECEIPTSDISBURSEMENTSSTOCKDateDocument numberQuantityLocationDocument numberQuantityDestinationQuantityUnit valueTotal value FigureAA3.2 Typical stock card example for laboratory storeroom Name and strengthUnitItem codeExpiration dateGlass slidesBox (100 slides)P500NoneAverage monthly consumptionMinimum levelMaximum level50150RECEIPTSDISBURSEMENTSSTOCKDateDocument numberQuantityLocationDocument numberQuantityDestinationQuantityUnit valueTotal value1 JanInventory5015 JanReq #15Lab4518 FebReq #320Lab2522 MarReq #105Lab206 AprReq #438Lab1210May LI-3100Lab store11215May Req #5025Lab8720 JunReq #5330Lab5730 JunReq #5925Lab321 JulReq #6215Lab173 AugReq #7017Lab0Sep0Oct04 NovSU-15200Lab store2006 NovReq #7230Lab1708 NovReq #7840Lab13031 DecInventory130 Using the stock card to calculate average monthly consumption Average monthly consumption (CA), adjusted for stock-outs (due to damage, expiry, or problems with delivery), is a measure of how much stock is used in an average month over a specific period. This information is critical for those responsible for calculating quantities to be procured or to be supplied by stores to an individual laboratory. Average monthly consumption can be calculated using the sum of quantities used or distributed over a period of time, normally 12months. Average monthly consumption, adjusted for stock-outs (CA), is defined as the average number of units used per month. The formula for calculating CA is: CA=CT [RM (DOS 30.5)] where: CT = total consumption during the review period RM = total consumption review period, in months DOS = number of days an item was out of stock during the review period 30.5 = average number of days in a month. Example Using the stock card for glass slides shown in FigureAA3.2, it is possible to obtain two pieces of information that will assist in calculating the average monthly consumption over 12months: The total consumption over 12months is obtained by summing all the disbursements made between 1January and 31December of the year covered by the stock card. The total consumption was 220boxes of glass slides. The number of days out of stock can be obtained by looking at the STOCK column. This shows that, from 3August to 4November, there were no boxes of slides in stock. Therefore, the number of days the item was out of stock was 92days. These values can be entered into the above equation to calculate CA, as follows: CA=CT [RM (DOS 30.5)] CA=220 [12 (92 30.5)] CA=220 [12 3.02] CA=220 8.98 CA=24.5 boxes Note: failing to take into account the number of days out of stock would give a lower (and inaccurate) result of 220 12=18.3. This would subsequently affect all other calculations and result in inaccurate quantification of needs for the laboratory. Minimum safety stock Safety stock is necessary to protect the storeroom from stock-outs; it also provides a safety net for variation within the procurement system. There is no single formula for calculating the safety stock level; however, this number is usually calculated from CA. For example, if stock is normally distributed every month and the laboratory wishes to have sufficient safety stock for two months to account for slower than normal delivery, safety stock would be calculated as follows: Safety stock = 2 CA = 2months extra for distribution the number of units per month. In the case of the glass slides, the safety stock required is: 2 24.5=49 boxes. When stocks are depleted to this level, the item must be reordered. To avoid stock-outs, the stock should not be allowed to go below the amount needed to cover two months. The approaches and strategies used to maintain adequate inventory levels should be reviewed. In the calculations presented above, the average inventory level can be lowered by reducing either the safety stock or the order quantity. However, if the order quantity is reduced, the item will need to be ordered more frequently to prevent stock-outs. This results in a reduced average inventory. More frequent ordering makes it easier to adapt to changes in demand and means that a lower level of safety stock is required. This reduction in the safety stock further reduces the average inventory. A shortened order interval increases some costs related to procurement, including: administrative costs there will be a greater workload in the ordering process; shipping and transportation costs more deliveries will be needed; and unit costs the cost per unit may be higher when purchasing smaller quantities. In summary, shortening the order interval can reduce the average inventory level and thereby reduce the costs of holding inventory. However, procurement costs may increase. Inventory control form The inventory control form is a form that allows the store or the individual laboratory to summarize the quantities and value of stock on hand. It also makes it possible to record discrepancies between the amount of stock that is actually on hand and the amount that should be on hand. An example of such a form is shown in FigureAA3.3. Stock may become unavailable due to stock-outs, deterioration, expiry, breakage or theft. Losses due to deterioration, expiry, breakage or theft often constitute a large part of the budget, so all means to reduce such losses should be taken. The quantification process can correct for, or at least minimize, stock-outs and losses due to over-stocking, but it cannot correct for the other factors. Used in conjunction with the list of expired laboratory supplies, the inventory control form is a critical record because it identifies stock losses that may have occurred at the storeroom level. Another method for checking for stock losses within the laboratory is to compare the amount of stock ordered for the laboratory against the test register. It can be difficult to check everything; therefore, it is best to select a few key high-value or problematic items, and monitor these regularly. Losses above those expected due to calibration of equipment or breakage may indicate some other cause of loss, which should be investigated. Loss from any cause should be minimized to make the laboratory more efficient. Money saved by minimizing stock losses can be used for improving or expanding services at no extra cost to the facility. Using the inventory control form This exercise should be performed at least once per quarter: Using the information on the laboratorys stock cards, enter the details for each product and the current balance. Assign a member of staff to make a physical count of each of the items that have been listed on the inventory control form. Enter the physical count in the appropriate column and compare with the quantities on the stock card. Record the discrepancies in the appropriate column In the example of a completed inventory control form (FigureAA3.4), there are discrepancies between the balance on the stock card and the physical count for five items. Possible reasons for such discrepancies could be that: the stock cards have not been accurately maintained; the physical stock count was inaccurate; or stock has been stolen from the storeroom. This should be further investigated and corrective action taken. FigureAA3.3 Inventory control: quarterly stock-taking form Page no: _____________ Name of store or laboratory _____________________________ Date completed: ____/____/____ Code no.Product nameStock unitQuantity on stock cardPhysical countUnit priceTotal price (physical count unit price)Discrepancy (stock card minus physical count)Expiration date Prepared by: ____________________________ Confirmed by: _______________________ Function or category: _______________________ Function or category: ___________________ FigureAA3.4 Example of completed inventory control form Name of store or laboratory _____________________________ Date completed: ____/____/____ Code no.Product nameStock unitQuantity on stock cardPhysical countUnit price (USD)Total price (physical count unit price)Discrepancy (stock card minus physical count)Expiration dateP500Glass slidesBox (500 slides)1301308.001040.00P511Sputum collection containersCarton (1000)725670.003920.0016R26Basic fuchsin powder100g8835.00280.00R28Auramine O powder50g7720.00140.00R27Methylene blue powder100g111060.00600.001R43Immersion oil100ml141420.00280.00P482Nichrome wireRoll (10metres)558.0040.00A28Disposable glovesBox (100)322510.00250.007T22Microscope bulb112825.00200.004R18Lysol disinfectantBottle (5litres)43437.00301.00T23Spare eyepiece for microscope162200.00400.004TOTAL7451.00 Prepared by: ____________________________ Confirmed by: _______________________ Function or category: _______________________ Function or category: ___________________ List of expired laboratory supplies A typical form for listing expired laboratory supplies is shown in FigureAA3.5. The list can be used to reveal two common problems: The expired items may have been over-stocked. Goods may have been received that have a shelf-life that is shorter than the reorder period. The first of these problems can be overcome by more accurate quantification. The second suggests either that orders should be placed more frequently, or that items with unduly short expiry dates should not be accepted, or both. A second use for the list of expired laboratory supplies is to find evidence of theft. To do this, users should compare the list of expired laboratory supplies with the inventory control form, subtracting the amount of expired stock from any discrepancy and allowing for normal wastage. The result can point to the possibility of theft. This information can then be used as the basis for investigating and taking necessary action to prevent such theft from happening again. A third use for the list of expired laboratory supplies can be to record expired, damaged or deteriorated stock that has been destroyed. In all cases, stock that has been returned or destroyed or is otherwise unusable should be deducted from the stock on hand in the stock records it does not constitute part of the regular use of that item. The data gathered in this list provide an indicator and can be used periodically to help manage the laboratorys work. The results can be sent to the procurement team with the consumption data. An example of a completed list of expired laboratory supplies is given in FigureAA3.6. Figure AA3.5 List of expired laboratory supplies Stock numberProduct descriptionExpiry dateQuantityUnit priceTotal price TOTAL: Date completed: ________ FigureAA3.6 Example of completed list of expired laboratory supplies Stock numberProduct descriptionExpiry dateQuantityUnit priceTotal priceR27Methylene blue powder, 100gMay 2005160.0060.00R28Auramine O powder, 50gJune 2009320.0060.00 TOTAL: USD 120.00 Date completed: ________ Annex A4 Additional resources This section lists resources that provide further, more detailed information on laboratory commodity and equipment management: Management Sciences for Health and World Health Organization. Managing drug supply, 2nd ed. West Hartford, CT, Kumarian Press, 1997. Rational Pharmaceutical Management Plus Program. Managing pharmaceuticals and commodities for tuberculosis: a guide for national tuberculosis programs (submitted to the United States Agency for International Development by the Rational Pharmaceutical Plus Program). Arlington, VA, Management Sciences for Health, 2005. Zagorskiy A, Owunna C, Moore T. Pharmaceutical management for tuberculosis assessment manual: revised edition of Drug management for tuberculosis assessment manual (submitted to the United StatesAgency for International Development by the Rational Pharmaceutical Plus Program). Arlington, VA, Management Sciences for Health, 2004. Walkowiak H, Gabra M. A commodity management planning guide for the scale-up of HIV counseling and testing services (submitted to the United StatesAgency for International Development by the Rational Pharmaceutical Plus Program). Arlington, VA, Management Sciences for Health, 2008. WHO Consultation on Technical and Operational Recommendations for Clinical Laboratory Testing Harmonization and Standardization, 2224 January 2008, Maputo, Mozambique. International Union Against Tuberculosis and Lung Disease (IUATLD). Technical guide: sputum examination for tuberculosis by direct microscopy in low income countries, 5th ed. France, IUATLD, 2000. Report Meeting on procurement of laboratory items, WHO/HQ, Geneva, 2728October 2008. Geneva, World Health Organization, 2008 ( HYPERLINK "http://www.who.int/diagnostics_laboratory/report_lab_procurement_oct08.pdf" http://www.who.int/diagnostics_laboratory/report_lab_procurement_oct08.pdf). WHO Prequalification of Diagnostics Programme ( HYPERLINK "http://www.who.int/diagnostics_laboratory/evaluations/en/" http://www.who.int/diagnostics_laboratory/evaluations/en/). Mundy C, Kahenya G, Vrakking H. The design and in-country evaluation of TB diagnostic kits, 20042006 (submitted to the Global TB Drug Facility and the United States Agency for International Development by the Rational Pharmaceutical Management Plus Program). Arlington, VA, Management Sciences for Health, 2006 ( HYPERLINK "http://www.stoptb.org/gdf/assets/documents/design_evalution_diagnostic_kits_GDF_2007.pdf" http://www.stoptb.org/gdf/assets/documents/design_evalution_diagnostic_kits_GDF_2007.pdf). Use of liquid TB culture and drug susceptibility testing in low and medium income settings. Summary report of the Expert Group Meeting on the use of liquid culture media, WHO Stop TB Department, Geneva, 26 March 2007. PART B Specifications for TB laboratory equipment, supplies and other commodities B1 Introduction to specifications The purpose of this part of the document is mainly to provide technical guidance for commodities used for diagnostic purposes in a tuberculosis (TB) laboratory. The document covers all commodities that is, equipment and supplies such as laboratory reagents, diagnostic kits and other consumables for all TB-laboratory activities, including microscopy, molecular tests, culture and drug susceptibility testing (DST). However, it does not cover commodities for research laboratories. ChapterB2 provides lists of commodities needed for the different laboratory activities. Chapters B3B5 cover the requirements for culture, DST and molecular biology in TB laboratories. Specifically, they provide the main specifications for equipment (ChapterB3), consumables (ChapterB4) and chemicals (ChapterB5). ChapterB6 provides detailed specifications for items used in molecular biology techniques. B1.1 Layout of lists The lists in ChapterB2 contain four columns, which provide the following information: the item number (column1); the descriptions and specifications for the item; that is, the quality requirements (column2); the number of items required (column3); and whether or not a detailed specification is available (column4). The item numbers in column1 are sometimes followed by a letter this denotes accessories for the item. For example, item2 in the list in SectionB2.1 is a Bunsen burner, and item2a is gas safety tubing for use with the burner. Alternatives to some items are also indicated in blue. Countries need to adjust and select alternative items as necessary, to fit local needs. Only certain items from the list will be required. The actual items needed and the quantities required will depend on the methods used, the workload and the design of the laboratory. Quantities indicated in this document are based on experience, and are suited for a national reference laboratory (NRL) performing about 12000cultures and about 1000first-line DST (FL-DST) and 100second-line DST (SL-DST); these quantities should serve as an estimate. For some consumables and chemicals, a fractionated delivery has to be included in the tender description, to take into account the shelf-life. Special delivery conditions (e.g.cool chain) must also be indicated in the tender documents. B1.2 Layout of detailed specifications For the detailed specifications (SectionB6), a format is used that can be adjusted, as necessary, to fit the needs of the country. The first column provides the specifications to be met in the tender; the second column provides space for the bidder to enter the specifications of the item offered. If required, a narrow third column can be added to record whether the item offered by the bidder meets the specifications, exceeds the specifications, or fails to meet the specifications but is still acceptable. The purchaser indicates the number or quantity of items required, while the bidder provides information about the manufacturer, type or model (or both), and country of origin. The section Description of function and use will include information about the equipments purpose and the laboratory area for which the equipment is intended. If necessary, this section will also contain comments on possible extension for additional use. Countries should adapt this paragraph to their specific use of the equipment. The main specifications contain all relevant technical data, including material to be used for manufacturing, acceptable noise levels, safety features, indicators desired to control correct functions, programs and so on. Electrical requirements should be adjusted to suit local needs for voltage, phase and so on, and the type of plug system commonly used in the country. B1.3 Manufacturers certificate In general, countries or laboratories procuring equipment should consider only manufacturers who produce their equipment under a strict quality management system (e.g. ISO9001 or better) . The main norms applying to medical devices especially for electrical engineering must be listed in the specifications. Country-specific norms should also be included. B1.4 Additional headings The other headings in the form are self-explanatory and may be summarized in the tender documents. They cover accessories, the operation and maintenance manual, installation and maintenance, standard maintenance tools, spare parts, packing data and customers tariff number, warranty and any other relevant remarks. The form gathers information beyond the specific technical specifications of each item. Depending on its purpose, the form can be simplified. For example, if a tender document is prepared for purchase of several different devices, most of the more general requests (e.g. certificates to be provided, installation and maintenance, after-sales service, operation and maintenance manuals, standard maintenance tools, warranty, etc.) will be included as separate paragraphs in the general tender document. For some organizations or nongovernmental organizations (NGOs) who buy and ship the equipment themselves, it will be helpful and more efficient for the information on Packing data and the customers tariff number to be available with each of the goods; these documents are needed for customs declaration and clearance. B1.5 Adjustment to local needs The specifications may vary according to the purpose of use. For example, where equipment will be used in the laboratory for both TB services and other investigations (e.g.use of a microscope to check blood films for diagnosis of malaria or other parasites), the specifications need to be adjusted accordingly. In many countries, appropriate equipment will depend on the environment and infrastructure; factors that should be considered include: climate (e.g. temperature, humidity, rainy seasons, thunderstorms); likelihood of earthquakes; whether the laboratory is easy to access (e.g.road conditions); and available infrastructure (e.g.clean water supply under sufficient pressure, electricity of constant voltage and gas supply). Environment and infrastructure conditions should be described in detail in the technical specifications for the equipment to be purchased. Any additional equipment that will be required for the laboratory equipment to function (e.g. air conditioning, water pump or uninterruptible power supply [UPS]) must be included in the tender documents. AnnexB1 describes factors that should be considered when evaluating the quality of the laboratorys electrical supply. AnnexesB2 and B3 describe requirements for selecting, installing and maintaining a biological safety cabinet (BSC); BSCs are critical for personnel safety in laboratories handling cultures of Mycobacterium tuberculosis. B2 Equipment, consumables and chemicals for microscopy laboratories TableB2.1 lists standard equipment required for one peripheral or microscopy laboratory, serving a population of 100000 people. Items 128 apply to all laboratories; items 2954 are additional equipment for microscopy laboratories preparing or procuring staining solutions for microscopy. Detailed specifications, where available, are given in SectionB6. Quantities should be adjusted to suit specific situations, such as the size of the laboratory, the workload and the external quality assurance policy. Table B2.1 Equipment for microscopy laboratories ItemDescription and specificationsNo. of itemsDetailed specifications1Binocular light microscope13Yes1aBinocular fluorescence microscope 1Yes2Bunsen burner Burner able to use natural gas, propane or butane gas, with a needle valve to regulate gasair mix; adjustable to economizer (mini) flame; gas hose connection; safety tubing with 9.5mm inner diameter; safe stand.22aGas safety tubing Tubing (length 0.51.5m) appropriate for the type of gas to be used and for safe connection to the gas hose connection; two hose clips for each tube.22bGas safety tubing 5m roll.1 2cButane gas cylinder Cylinder sizes selected according to the size of the available storage containers. Cylinders stored according to national rules; cylinders larger than 500ml stored in a well-ventilated locked box, connected to the laboratory by a metal pipe. Inside the laboratory, flexible connections as short as possible.22dPressure reducer If gas cylinders are used, pressure reducers are needed with appropriate tube fittings and stopcock (one stopcock is required per laboratory with five spare stopcocks kept in stock).23Alternative to item2: Spirit lamp (stainless steel) Provide an alternative to Bunsen burners if gas is not available. Lamps made of stainless steel; volume approximately 85ml; dimensions approximately 100mm (diameter) and 65mm (height); with a wick (cotton wool or metal wire), a method of wick adjustment and a cap.23aAlternative to item3: Spirit lamp (sodalime glass) Provide an alternative if stainless steel spirit lamps are not available. Lamps made of sodalime glass (composition 7274% SiO2, 11.9% Al2O3, 0.090.1%Fe2O3); volume approximately 100ml; dimensions approximately 75mm (diameter) and 100mm (height); with a wick (cottonwool or metal wire) and a seat-grinded cap.24Loop holder Loop holders with an upper part made of aluminium or stainless steel and handle made of plastic (240mm long).35Rack for loop holders Disinfectable racks able to hold 34 loop holders, with a proper stand.16Loop Closed loops of 34mm diameter, made of nickelchromium alloy wire 0.81mm in diameter and heat resistant to 1200C.47Staining rack Constructed in the laboratory from glass rods and rubber or silicon tubes, or from wire (preferably stainless steel) of appropriate size. Racks to have a bowl (made of glass or stainless steel) to collect staining solutions.128Slide drying rack Constructed in the laboratory from metal or granite (not wood); if bought, to be of metal or heat-stable plastic (stable at e"120C). Racks to have 20 30 positions and a slight incline so that slides are easy to remove. 1 9Slide storage box Boxes able to hold 100 slides (slides 76mm 26mm), with a sturdy lock, a cork-lined base and a flap cover with an index-card holder.410Scissors Stainless steel (18/8), polished; one blade sharp, one blade blunt; 160mm long; autoclavable.111Anatomical forceps Stainless steel (18/8); antimagnetic; rounded; 160mm long.112Pressure cooker Staff to purchase the largest size available from local suppliers.113Timer Features to include spring-driven motor, 060-minute timer and a disinfectable plastic housing. Timers to have a sound (e.g. buzzer or bell) to signal time elapsed, a stand and a clip to attach them to laboratory gowns. 114Laboratory gown Cotton, long sleeved and white; available in a selection of sizes appropriate for local staff.1 per person 15Pen Blue or black ballpoint pens.216Pen Red ballpoint pens.117Marker pens Fast-drying, black, thick-line (0.81mm) marker pens that can write on most surfaces (even when the surface is cold and wet); ink resistant to water, alcohol and autoclaving.218Alternative to item 17: Self-adhesive labels Labels in rolls or sheets (e.g. 50mm 19mm labels, 24 per sheet and 200 sheets per box=4800 labels); strong adhesive. 1 box19Reagent bottle Bottles of 1litre; made of amber borosilicate glass; GL45 thread and cap; compliant with ISO4746 (Oxygen-free copper scale adhesion test).420Staining bottle Polyethylene flasks with a narrow neck; with a screw cap, swan neck or jet dispenser; volume required (250ml or 500ml) will depend on the number of slides stained per day.321Beaker 1litre plastic polypropylene (PP); with handles. 122Funnel Made of sodalime glass or PP; upper diameter of approximately 100mm. 323Alternative to item 22: Stainless steel funnel Upper diameter of approximately 100mm; with a handgrip and an air drain.324Sea sand 1kg, washed (for cleaning loops).125Container Thick walled; made of glass; screw cap and wide neck (to hold sand and alcohol for cleaning loops).126Drop bottle Made of sodalime glass; complete with exchangeable norm-grinded pipette and rubber suction device; volume 100ml.127Oil dropper bottle 10ml; made of low-density polyethylene (LDPE); with a sealed stopper fixed to a screw cap.128Metal rod 30cm long; used to heat slides on staining racks.52 Additional equipment for microscopy laboratories preparing or procuring staining solutions for microscopy 29Water distiller1Yes30Balloon 30litres; made of high-density polyethylene (HDPE); with stopcock (to store distilled water).131Analytical balance, with set of weights 1Yes32Magnetic stirrer with heating plate 1Yes33Stirring rod Magnetic; cylindrical; coated with polytetrafluorethylene; at least 10mm wide and 60mm long.334Jerry can for stain storage 2030litres; made of HDPE; with spout and ventilation cap.635Jerry can for stain supply 35litres; made of HDPE; double leak-proof cap.3 36Stain stock bottle 1litre; made of HDPE (opaque or coloured); double leak-proof cap.As needed37Plastic bottle3 38Transport container Used if specimens are to be sent to another laboratory; must meet P650 packaging requirements (Guide to the Packaging & Transportation of Biological Specimens by Road, available from http://www.izvg.co.uk/regulations.pdf).As needed393litre conical flask (Erlenmeyer) Made of borosilicate glass 3.3; thick walls and narrow mouth; graduated.6401litre conical flask (Erlenmeyer) Made of borosilicate glass 3.3; thick walls and narrow mouth; graduated. 3411litre measuring cylinder Made of borosilicate glass 3.3 class A; autoclavable; 0.5ml graduated.1042500ml measuring cylinder Made of borosilicate glass 3.3 class A; autoclavable; 2.5ml graduated.1043250ml measuring cylinder Made of borosilicate glass 3.3 class A; autoclavable; 1.0ml graduated.1044100ml measuring cylinder Made of borosilicate glass 3.3 class A; autoclavable; 1.0ml graduated.104550ml measuring cylinder Made of borosilicate glass 3.3 class A; autoclavable; 0.5ml graduated.104625ml measuring cylinder Made of borosilicate glass 3.3 class A; autoclavable; 0.25ml graduated.1047Laboratory glassware drying rack Wall mounted; made of polystyrene; with at least 24pegs for cylinders and conical flasks.148Bottle brush Brush at least 120mm long; total length (brush and handle) approximately 480mm.1049Chemicals spoon Medium size (2025cm); made of polished stainless steel; spoon on one side and spatula on the other.250Filtration funnel Made of soda lime glass or PP; diameter approximately 160mm; total height approximately 195mm.251Filter paper Qualitative, retaining particles e"8m; diameter 240mm; folded (prepleated).10052Household water filter Stainless steel unit with a tap and a ceramic candle filter; each compartment should hold at least 5litres of water.153Beaker with handle 1litre; made of PP; graduated.2 Table B2.2 lists consumables needed in the microscopy laboratory. The actual quantities required will depend on the number of smears performed. Quantities indicated are based on an estimate of 10000 slides per year and need to be adjusted to the actual workload of the laboratory. Table B2.2 Consumables for microscopy laboratories ItemDescription and specificationsNo. of itemsDetailed specifications1Alternative to item 6, Table B2.1: Wooden applicator stick Can be used if loops are not available.10 packs of 10002Microscope slides Made of sodalime glass; 76mm 24mm; 1.01.2mm thick; compliant with ISO80371 (Optics and optical instruments Microscopes - Slides Part 1: Dimensions, optical properties and marking); cleaned and degreased; straight edges and corners; cellophane wrapped and tropical packing (i.e. sheets of paper between each slide).200 packs of 503Alternative to item 2: Microscope slides As described in item 2, but with a 20mm frosted end (so that slides can be marked with pencil).2004Diamond pen To mark glass sides; handle made of plastic or wood.25Sputum container Break resistant, disposable and made of transparent plastic (PE or PP) that can be burnt without releasing toxins; wide mouth (diameter e"35mm); volume 40 50ml; tight, waterproof, screw cap; frosted writing panel (if possible).100006Immersion oil 500ml glass bottle of nondrying, synthetic oil with a refractory index of 1.515 1.517; a viscosity at 20C of 100 120mPas; nonfluorescent; light transparency at 400 nm of e"75%.17Lens tissue (or lens paper) Soft, lint-free tissue; approximately 80mm 100mm (sold in blocks of 50sheets with 10blocks per pack).5packs8Handwashing soap Preferred type is liquid soap distributed from a mechanical, wall-mounted dispenser.At least one per sink9Paper towel Single use; approximately 230mm 300mm; folded, to be dispensed individually from a wall-mounted box (sold in packs of 150 towels per pack, 30 packs per carton).1carton10Option: Paper towel box Wall-mounted, refillable box that can hold at least 150paper towels.211Gloves Powder-free, latex-free disposable gloves made of vinyl (clear) or nitrile (blue); rated as CE category III (for complex risks) and with a textured surface for slip resistance; e"0.3mm thick and e"240mm long; stocked in sizes (small, medium and large) appropriate for the laboratory staff (sold in boxes, with 100gloves per box). 24 boxes12Option: Nitrile gloves Recommended for use if staining with Auramine O is performed. Powder-free, latex-free disposable gloves; rated as CE category III (against chemical risks and resistant to ethidiumbromide or auramine staining); e"0.3mm thick and e"249mm long; stocked in sizes (small, medium and large) appropriate for the laboratory staff (sold in boxes, with 100gloves per box).Depending on use of Auramine O13Laboratory request form1500014Laboratory report form1500015Laboratory register131670mm filter paper Round cellulose filter paper, type 113A; weight 80g/m; mediumfast filtration; retaining particles e"8m; 70mm diameter (sold in packs, with 100sheets per pack).4 packs17150mm filter paper Round cellulose filter paper, type 113A; weight 80g/m; medium fast filtration; retaining particles e"8m; 150mm diameter (sold in packs, with 100sheets per pack).4 packs18Disinfectant 5kg bottle of phenol technical-grade disinfectant.319Stable chlorine disinfectant Sodium dichloroisocyanurate; 6g tablets with 1g active chlorine per tablet; usually dissolved with one tablet per 5litres of warm water (sold in packs with 100 tablets per pack). 2 packs20Goggles Meeting EN166 and EN170; adjustable frames (adjustable to at least three positions); nonslip strap; integrated side and top protection; total weight <35g.221Option: Centrifuge tube The national tuberculosis programme (NTP) may require sputa or specimens from selected patients to be sent to a central laboratory for culture. Transport may be in 50ml graduated centrifuge tubes, made of PP, sterile, with screw cap (sold in packs, with 500tubes per pack).10 packs TableB2.3 lists chemicals needed for a microscopy laboratory. Quantities indicated are based on an estimate of 10000 slides per year. They need to be adjusted to the actual workload of the laboratory (i.e. the number of smears performed). Table B2.3 Chemicals for microscopy laboratories ItemDescription and specifications No. of items1Methylated ethanol Low-grade, required for spirit lamps (sold in 2.5litre bottles).42Basic fuchsine C20H20ClN3 Certified, with colour index (CI) 42510; molecular weight (MW)337.85g/mol; melting (decomposition) point 200C; main product component e"80% (sold in 100g bottles).33Methylene blue  C16H18ClN3SxH2O (where x=2 3) Certified, with CI50015; MWd"319.86g/mol; melting (decomposition) point ~180C; dye component e"82%% (sold in 100g glass bottles, including counterstain for Auramine O).14Colourless phenol crystals  C6H6O Pure grade (e"99% pure); MW94.11g/mol; melting point 40.8C (sold in 5kg bags). (Note: crystals may become light reddish over time.)15Ethanol for stain solutions  C2H6O Technical-grade ethanol (96% pure); MW46.07g/mol; boiling point 75 78C (sold in 2.5litre bottles).26Ethanol for decolourization  C2H6O Technical-grade ethanol (96% pure); MW46.07g/mol; boiling point 75 78C (sold in 2.5litre bottles).207Hydrochloric acid  HCl Of e"35% HCl, density e"1.17g/cm3; MW 36.5g/mol (sold in 2.5litre bottles).18Alternative to item 7: Concentrated sulfuric acid  H2SO4 Technical-grade sulfuric acid; density 1.84g/cm; MW98.08 (sold in 2.5litre bottles).1Additional chemicals for fluorescence microscopy9Auramine O  C17H22ClN3 Certified, with CI41000; MW 303.84g/mol; melting point ~265C; dye component e"85 (sold in 50g bottles).110Potassium permanganate  KMnO4 Analytical grade (99% KMnO4); MW158.04g/mol; decomposition >240C (sold in 250g bottles). (Note: potassium permanganate is a strong oxidant; it can be replaced by methylene blue or diluted blue ink.)1 B3 Main specifications for equipment for culture, DST and molecular biology TableB3.1 lists equipment needed for culture, DST and molecular biology laboratories. Indicated quantities are based on experience and are suited for an NRL performing approximately 12000 cultures and about 1000FL-DST (and 100SL-DST). They should serve as an estimate some laboratories may need additional or alternative equipment; also, the actual items and quantities needed will depend on the methods chosen, the workload and the design of the laboratory. Table B3.1 Equipment for culture, DST and molecular biology laboratories ItemDescription and specificationsNo. of itemsDetailed specifications1Biological safety cabinet Class I or class II BSC; minimum width 120cm (4ft); recommended width at least 150cm (5ft).2Yes1aBase support For BSC.21bUPS For BSC; battery pack should last 20minutes.22Mini-shaker or vortex3Yes3Alternative to item 2: Overhead stirrer Able to fit at least 10 50ml tubes.23aUniversal adapter For overhead stirrer.24Test-tube rack Made of 18/8 stainless steel; to hold two rows of 12 test-tubes (test-tubes with diameter 16mm or 18mm).55Test-tube rack Made of 18/8 stainless steel; to hold two rows of 4 test-tubes (test-tubes with diameter 35mm).56Safety gas burner With a glass chimney and a foot switch to operate the ignition and gas supply.2Yes6aSafety gas tubing With safety clamps on both ends; inner diameter 9mm; length750mm.27Alternative to item 6: Electric micro-incinerator for loops2Yes84 mminoculation loop Made of platinumiridium wire with diameter 0.60.7mm; total length 5cm; closed loop with diameter 4mm.88a3 mm inoculation loop Made of platinum-iridium wire with diameter 0.60.7mm; total length 5cm; closed loop with diameter 3mm, holding 10l for DST.88bLoop holder Upper part made of aluminium or stainless steel and handle made of plastic (240mm long).128cRack for loop holder Disinfectable; able to hold 34 loop holders; with a proper stand.49Alternative to item8: Disposable loop Sterile and approximately 220mm long (sold in packs of 1000 loops).15 packs10Stainless steel bucket 10litres with lid; height 250mm; upper diameter 280mm; lower diameter 185mm.511Discard bottle Made of stainless steel or thick-walled glass; screw cap.612Funnel Made of 18/8 stainless steel, with an air drain; diameter of ramp approximately 15mm; top diameter approximately 120mm; total height approximately 165mm.613Safety box For sharps; made of PP; volume 3litres; self-opening lid and safety lock.514Laboratory chair Adjustable (vertical adjustment 480540mm); seat and back made of polyurethane foam; disinfectable; five wheels; no armrests.215Mobile container Disinfectable; preferably made of PP and set on four roller castors (two lockable); approximate dimensions 600mm wide 550mm deep 750mm high; high resistance to chemicals and temperature (to 90C); light-fast and colour-fast; worktop 30mm thick, with edges rimmed with impact-resistant PP; four drawers that cannot be totally extracted.216Incubator Capacity e"700litre; temperature range 30 70C; calibrated to 37C.2Yes16aShelf for incubator Made of perforated stainless steel (if not included with the incubator).417Centrifuge Refrigerated bench-top centrifuge with aerosol-free buckets; swing-out rotor with capacity for 1litre of liquid to be centrifuged at >3200G.1Yes17aSwing-out rotor For bench-top centrifuge; four place.117bRotor bucket417cAerosol-tight lid for rotor bucket417dReducing adapter For 50ml Falcon tubes, or equivalent tubes.417eReducing adapter For 15ml or 12ml Falcon tubes, or equivalent tubes.418Autoclave Basic vertical unit with capacity of approximately 75litres (or larger).2Yes18aProgram control unit A unit to enable fully automated process control, with temperature up to 134C.218bExhaust air filtration system Forced-air filter (1.0mm pore size) for autoclave and vacuum pump.118cLiquid temperature-control unit With temperature probe and pressure-sealed duct.118dPressure device For sterilization of liquid.118eAutoclave recooling system To shorten the time taken to sterilize liquids.118fWire basket or stainless steel bucket For autoclave.218gWire basket with drip tray For autoclave.219Hot-air oven Temperature range 30250C, approximately 110 or 250litres (size depends on the number of reusable items and amount of glassware).1Yes19aShelf for 254litre hot-air oven Perforated stainless steel (if not delivered with the incubator).220Ion-exchanger cartridge Able to support a flow rate of approximately 950l/hour; quick connection coupling; exhaust; excess pressure valve (2.510bar).221Digital conductivity measuring device To measure the quality of laboratory-grade water.222Ion-exchanger resin For laboratory-grade water; pack of 5נ10litre resin; with filter bag to store used resin.523Water distiller Fully automatic; capacity of 412l/hour; producing distilled water with conductivity of approximately 3S.1Yes2430litre balloon HDPE winding with discharge cock; 30litres (additional storage tank for distilled water).12510litre balloon HDPE winding with discharge cock; 101itres (for distribution of distilled water).426Stopcock PP HDPE winding for HDPE balloon.1027Test-tube rack To hold 60 16mm diameter test-tubes; made of PP; temperature resistant (15 C to 130C); autoclavable; approximately 250mm wide 105mm deep 70mm high.1228Alternative to item 27: Test-tube rack If 18mm diameter test-tubes are purchased, test-tube rack must be adjusted to match.15029Alternative to items 27 and 28: Rack to hold 28 universal bottles 15030Alarm clock or digital countdown timer With a memory function and a countdown (2digit display), with an alarm at 0minutes.3311ml pipettes Conformity certified; graduation of 0.01ml (sold in packs of 10pipettes).3 packs322ml pipettes Conformity certified; graduation of 0.02ml (sold in packs of 10pipettes).3 packs335ml pipettes Conformity certified; graduation of 0.05ml (sold in packs of 10pipettes).3 packs3410ml pipettes Conformity certified; graduation of 0.1ml (sold in packs of 10pipettes).3 packs35Short pipette For work in BSC; approximately 25cm long; 0.5ml; graduation 0.01 (quantity adjusted according to dilution and inoculation schema for DST).20036Pipette box Square, made of aluminium, with lid; suitable for pipettes 2025cm long and for work in BSC; silicon insert; disinfectable; autoclavable up to 205C.1037Pipette box Square, made of aluminium, with lid; suitable for pipettes 35cm long; silicon insert; disinfectable; autoclavable up to 205C.1038Inlay Teflon, for pipette box.2039Safety pipetting ball Standard.1040Automatic pipette aid For use with pipettes and pasteur pipettes (volumes between 0.1ml and 1.0ml); rechargeable, with an 8-hour battery capacity and a low-battery indicator; pipetting velocity adjustable (at least 3 speeds); weighing less than 200g. 241Alternative to item 40: Set of mechanical pipetting aids For standard pipettes (up to 0.2ml, up to 2ml and up to 10ml); thumb wheel and quick-release mechanism.242Pipette washer With automatic siphon system; made up of one cylindrical washing container with siphon outlet, a connecting tube and two pipette baskets (as inserts); total height approximately 750mm, diameter approximately 170mm.143Desiccator Made of borosilicate glass; total height approximately 150mm; inner diameter approximately 175mm; lid with stopcock and screw cap (with a GL32 thread).244Membrane vacuum pump For desiccator; suction >15l/hour and pressure endpoint <20mbar.145Woulff flask or bottle 1litre, made of borosilicate glass with a screw cap (thread GL45) and a silicone ring; height adjustable; two necks, each with an 8mm outer diameter and a screw cap (thread GL14).146Vacuum tube 3m long.147Silica gel For desiccator (sold in 0.5kg bags).1 bag48Transport box Boxes to transport specimens; boxes should meet P650 packaging requirements1049Alternative to item 48: Cool box for sputum transport Boxes to transport sputum by car for long travel times in areas with extreme temperature variation over the year; able to cool to 20C below ambient temperature and heat to 40C above ambient temperature, using Peltier elements; with a 12V plug to fit a car cigarette lighter and a car cable (length e"2.5m); able to hold approximately 27litres; inner dimensions e"335mm (width), e"230mm (depth) and e"380mm (height); inside and outside surfaces made of a durable and disinfectable material (e.g.LDPE); insulated with polyurethane foam; meeting P650 packaging requirements.350Additional to items 48 and 49: Sturdy container Sturdy containers with tight-fitting lids and leak-proof covers, holding at least 1litre; made of PP with a gasket-tightened screw cap, or from stainless steel with a tightly fixed cover; meeting P650 packaging requirements.1050aSorbent material Material to buffer specimen containers when packed into transport boxes (see item48) (sold by the kilogram).10kg51Electronic maximaminima thermometer Electronic, two-channel thermometer for measuring range of room temperature (10C to 50C); with battery.1052Plastic-foil welder 30cm.153Dispenser Wall-mounted dispenser for 1litre bottles (two per sink: one for liquid soap and one for disinfectant).2 per sink54Paper-towel box Wall-mounted, refillable box that can hold at least 150paper towels.455Goggles Goggles meeting EN166 and EN170; frames adjustable to at least three positions; nonslip strap; integrated side and top protection; total weight <35g.556Emergency spill kit1Yes57First-aid kit Designed specifically for scientific laboratories, including an eye-rinse bottle; store in a wall-mounted, lockable cupboard that has a gasket-sealed door.158Thermic anemometer With telescope probe.1YesMedia kitchen (additional equipment)59Analytical balance 1Yes60Precision balance1Yes61pH meter 1Yes62Bunsen burner With fine tubing.263Tripod stand 164Water-bath 20litres, with cover; temperature regulation controlled by a microprocessor.165Magnetic stirrer With heating plate.1Yes66Magnetic stirring bars Set of 10: 1 15mm, 2 20mm, 2 25mm, 1 30mm, 2 40mm, 2 50mm; round and coated with polytetrafluoroethylene (PTFE).167Filling station for sterile culture media 1litre reservoir (Squibb sedimentation funnel or cylindrical dropping funnel) with graduation lines and tube for pressure exchange; outlet tube with 6mm diameter; glass stopcock and PE stopper.468Filling station for sterile culture media 250ml reservoir (Squibb sedimentation funnel or cylindrical dropping funnel) with graduation lines and a tube for pressure exchange; outlet tube with 4mm diameter; glass stopcock and PE stopper.1069Alternative to item 68: Automatic filling station Peristaltic pump (1350rpm) for sterile dispensing of culture media, with a four-roller, easy-load pump head; pump to include a 15m silicon tubing bore (1.6mm in diameter) and run on 230 10 V AC/50Hz; pump to store at least five dispensing programs, dispensing a minimum dose of 3.5 0.5ml and a maximum dose of 15 2ml, with accuracy 1%. Pump to be compliant with standards IEC3351 and EN60529.170Stative set Plate plus rod and clamp.371Inspissator To coagulate egg-based culture media; capacity for e"350 tubes; able to run on 230 10V AC/50Hz.1Yes72Refrigerator 300litres, suitable for environments with ambient temperatures up to 43C; including a 60litre freezer ( 22C); energy efficiency class A+; able to run on 230 10 V AC/50Hz.2Yes73Floor-standing refrigerator 150litres with 20litre freezer (22C); energy efficiency class A+; able to run on 230 10V AC/50Hz.1Yes74Floor-standing refrigerator 300litres, cooling to 4C; energy efficiency class A+; able to run on 230 10V AC/50Hz.3Yes75Option: Chest freezer For laboratories preserving strains for national or scientific purposes; 70litres, with temperature range 50C to 85C; complete with cassettes and racks.1Yes76Stainless steel funnel Short, wide stem; upper diameter of approximately 150mm; with an air drain.577Swan-neck bottle (flask) PE bottle with narrow neck, screw cap and water dispenser (sold as a set comprising one 250ml, one 500ml and one 1litre bottle).5 sets78Bowl Plastic, approximately 500mm 350mm.5792litre conical flask (Erlenmeyer) Graduated; made of borosilicate glass 3.3; wide neck; autoclavable; compliant with ISO3585.2801litre conical flask (Erlenmeyer) Graduated; made of borosilicate glass 3.3; wide neck; autoclavable; compliant with ISO3585.1081500 ml conical flask (Erlenmeyer) Graduated; made of borosilicate glass 3.3; wide neck; autoclavable; compliant with ISO3585.1082250ml conical flask (Erlenmeyer) Graduated; made of borosilicate glass 3.3; wide neck; autoclavable; compliant with ISO3585.1083100ml conical flask (Erlenmeyer) Graduated; made of borosilicate glass 3.3; wide neck; autoclavable; compliant with ISO3585.208450ml conical flask (Erlenmeyer) Graduated; made of borosilicate glass 3.3; wide neck; autoclavable; compliant with ISO3585.20851litre measuring cylinder Class A; made of polymethylpentene (TPX); autoclavable; compliant with ISO6706; 10.0ml graduated.586500ml measuring cylinder Class A; made of TPX; autoclavable; compliant with ISO6706; 5.0ml graduated.587250ml measuring cylinder Class A; made of TPX; autoclavable; compliant with ISO6706; 2.0ml graduated.588100ml measuring cylinder Class A; made of TPX; autoclavable; compliant with ISO6706; 1.0ml graduated.108950ml measuring cylinder Class A; made of TPX; autoclavable; compliant with ISO6706; 1.0ml graduated.590Thick-walled glass flask 2.5litres; flanged rim; for preparation of culture medium.6912.0litre bottle (transparent) Graduated; made of transparent borosilicate glass 3; to include a ring and cap of PP (GL45 thread); autoclavable; compliant with ISO 3585 and ISO4796.3921.0litre bottle (transparent) Graduated; made of transparent borosilicate glass 3.3; to include a ring and cap of PP (GL45 thread); autoclavable; compliant with ISO 3585 and ISO4796.1093500ml bottle (transparent) Graduated; made of transparent borosilicate glass 3.3; to include a ring and cap of PP (GL45 thread); autoclavable; compliant with ISO 3585 and ISO4796.5942.0litre bottle (amber) Graduated; made of amber borosilicate glass 3.3; to include a ring and cap of PP (GL45 thread); autoclavable; compliant with ISO 3585 and ISO4796.3951.0litre bottle (amber) Graduated; made of amber borosilicate glass 3.3; to include a ring and cap of PP (GL45 thread); autoclavable; compliant with ISO 3585 and ISO4796.296Screw cap With welded-in PTFE membrane (0.2m pore size); autoclavable to 40C.1097100ml laboratory beaker Made of ethylene tetrafluoroethylene (ETFE); heat stable up to 150C; low form with graduation and spout.1098250ml laboratory beaker Made of ETFE; heat stable up to 150C; low form with graduation and spout.1099600ml laboratory beaker Made of ETFE; heat stable up to 150C; low form with graduation and spout.101001litre laboratory beaker Made of ETFE; heat stable up to 150C; low form with graduation and spout.31012litre laboratory beaker Made of ETFE; heat stable up to 150C; low form with graduation and spout.31021litre volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.5ml.5103500ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.25ml.10104250ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.15ml.10105200ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.15ml.15106100ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.1ml.1010750ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.08ml.1510825ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.06ml and adjusted to In.510920ml volumetric flask Class A; made of borosilicate glass 3.3 with PE stopper; autoclavable; error margin 0.06ml and adjusted to In.25110Drying rack for laboratory glassware Wall-mounted polystyrene drying rack, with at least 24 pegs for cylinders and conical flasks.1111Funnel Short stem; diameter 75mm; made of PP.5112Funnel Short-stem; diameter 125mm; made of PP.5113Powder funnel Stem diameter 100mm.51145mm diameter glass bead Made of massive glass.21153mm diameter glass bead Made of massive glass.1116Pyrex tubes Thick-walled; autoclavable; with ISO winding and screw cap with tight gasket; 16mm 100mm (sold in packs of 100).5 packs117Strainer Stainless steel; diameter 20cm; including one grip and two rests.1118Set of spatulas Each approximately 150mm long (with blades approximately 40mm long); widths ranging from 3mm to 9mm; made of electrolytically polished 18/8 stainless steel.6119Forceps Straight; pointed; made of polished 18/8 stainless steel.5120Optional: Laboratory washer For laboratory glass; with upper and lower baskets.1120aWasher 1/2 insert With 28 small spring hooks.1120bWasher 1/2 insert For baskets.1120cWasher upper basket For nozzles.1120dWasher 1/4 insert For test-tubes 160נ16mm.2120ePhosphate cartridge With first refill.1120fWater supply Separate supply for deionized water; including a conductivity meter with digital display; hoses for inflow and outflow.1120gAqua purificator cabinet To hold two demineralizing cartridges.1Molecular biology 121Polymerase chain reaction (PCR) workstation Working under UV light.1Yes122Thermocycler To hold 96 tubes. 1Yes123Centrifuge For standard reaction tubes; at least 15000G.1Yes124Ultrasonic bath 1Yes125Thermo-shaker For use in hybridisation; to hold at least 12 tubes (but preferably 48 or 96 tubes).1Yes126Optional: Electrophoresis Horizontal minigel system. 1Yes127Optional: Power supply For electrophoresis.1Yes128Optional: UV-light illuminator With window at least 200mm 200mm.1129Test-tube rack Autoclavable; able to hold at least 20test-tubes (1.52ml test-tubes, 10mm diameter).10130Floating rack For ultrasonic baths; to hold 20 reaction containers (2ml containers, 10mm diameter).2131Deep-freeze storage box Autoclavable; made of PP; with lid; able to hold 0.2ml PCR tubes (as single tubes, 8-tube strips or 12-tube strips) and 96-well PCR plates.5 132Rack with plastic lid Autoclavable racks to hold cryovials (2ml); suitable for temperatures 196C to 121C.50133Deep-freeze storage box Autoclavable; to hold cryovials; made of PP; with a lid, including a panel to record contents.101341-channel pipette Variable from 0.5l to 10l.3Yes1351-channel pipette Variable from 10l to 100l.4Yes1361-channel pipette Variable from 100l to 1000l.4Yes137Rack To hold at least four 1-channel pipettes.4138Optional: 8-channel pipette 550 L (accuracy at least 41.5% when the first value applies to smallest volume, last one to the largest volume in the stated range, precision at least 30.6% when the first value applies to smallest volume, last one to the largest volume in the stated range).1139Optional: 8-channel pipette 50300L (accuracy at least 1.5%1%, precision at least 0.80.5%).1140Stand for multichannel pipettes1141Hand-dispenser pipette Suitable for combi-tips, with five dispensing steps.2142Tripod stand For 2litre waste bags.5143Optional: Microwave oven or water-bath Microwave oven of approximately 900W; inner volume e"20litres.1144Refrigerator With freezing compartment.1145Freezer1Use of liquid media146Mycobacterial growthindicator tube (MGIT) system To detect mycobacterial growth in liquid media.1147UPS For the MGIT.1Office148Computer1149Combined printer/fax/photocopier1150UPS For the computer.1151Consumables Except paper.1152Request and report form sheetsAs needed B4 Main specifications for consumables for culture, DST and molecular biology TableB4.1 lists consumables needed for culture, DST and molecular biology laboratories. Indicated quantities are based on experience and are suited for an NRL performing approximately 12000 cultures and about 1000FL-DST (and 100SL-DST). They should serve as an estimate quantities should be adjusted to the actual workload of the laboratory and the methods used. Table B4.1 Consumables for culture, DST and molecular biology laboratories ItemDescription and specificationsNo. of itemsDetailed specifications1Culture tubes 125mm long; outer diameter 16mm or 18mm and inner diameter at least 13mm; made of borosilicate glass; at least 1mm thick; with an external thread; autoclavable screw cap made of PP or Teflon, with a sealing gasket (sold in packs of 100).260 packs1aAlternative to item 1: 28ml universal glass bottles Straight neck (no shoulder) made of heavy-duty glass; either an aluminium screw cap with rubber lining or an autoclavable screw cap made of PP or Teflon, with a sealing gasket (sold in packs of 100).260 packs250ml PP tubes for centrifuge Tight screw cap; size 115mm 30mm (sold in packs of 500). 60 packsYes315ml PP tubes for centrifuge Tight screw cap; size 120mm 17mm (sold in packs of 500).4 packs4Plastic pasteur pipettes Graduated; holding 1.5ml; disposable and sterile (sold in packs of 500).30 packs5Optional for strain bank: Cryovials Graduated 2ml vials with screw cap; vials round with an external thread; made of PP with a frosted marking area; sterile and able to be sterilized with -rays; stable at  196C to 121C; free from ribonuclease (RNase), deoxyribonuclease (DNase), deoxyribonucleic acid (DNA) and endotoxins (sold in packs of 1000).1 pack6Small gloves Powder free; disposable; made of vinyl or nitrile; 0.16mm thick and >240mm long (sold in packs of 100).60 packs7Medium gloves Powder free; disposable; made of vinyl or nitrile; 0.16mm thick and >240mm long (sold in packs of 100).90 packs8Large gloves Powder free; disposable; made of vinyl or nitrile; 0.16mm thick and >240mm long (sold in packs of 100).30 packs9Bags for waste bins Capacity approximately 24litres; made of transparent PP at least 40m thick; autoclavable up to 134C; meeting the countrys standards for environmental and hygiene considerations (sold in packs of 1000). 1 pack10Stand To hold small plastic bags with a 2litre capacity.511Bags For noninfectious material; made of transparent PP; size 200mm 300mm (sold in packs of 100).1 pack12Syringes 20ml; graduated; with Luer connection and a piston lock; made of colourfast PP; for single use only; sterile; individually wrapped; compliant with ISO 78861 (sold in packs of 100).1 pack13Syringe filter Hydrophilic and at least 25mm in diameter; pore size d"0.22m; with Luer connection; suitable for pressure up to 5bar; for single use only; sterile and individually wrapped (sold in packs of 100).1 pack14Filter paper Round; weight approximately 75g/m; diameter 185mm; able to retain particles >4m (sold in packs of 100).3 packs15Filter paper Round; weight approximately 75g/m; diameter 150mm; able to retain particles >4m (sold in packs of 100).6 packs16Marker pen Fast drying, black, thick line (0.81mm); able to write on most surfaces (even when the surface is cold and wet); ink resistant to water, alcohol and autoclaving.317Adhesive labels (Sold in rolls of 5000 labels).2 rolls18Cryotags To fit cryovials and standard microtubes (sold in rolls of 1000).5 rolls19Sterile indicator tape Approximately 19mm wide 55m long, for hot-air sterilization (green/brown).5 rolls20Sterile indicator tape Approximately 19mm wide 55m long, for autoclave (beige/dark brown).5 rolls21Aluminium foil 30m thick 600mm wide (sold in 100m rolls).3 rolls22Parafilm For use at 40C to 50C; 100mm wide 58m long; with dispenser.3 rolls23Plastic-foil rolls 30cm wide.5 rolls24Tube brush At least 280mm long (brush 80mm long) 20mm in diameter.2025Glassware brush Approximately 480mm long (brush 120mm long) 60mm in diameter.1026Large laboratory coat Long sleeved; white; made of cotton; with back opening.1027Medium laboratory coat Long sleeved; white; made of cotton; with back opening.2028Small laboratory coat Long sleeved; white; made of cotton; with back opening.1029Face masks Fit Factor for Inert Particle (FFP) FFP2 or FFP3 face masks (depending on national policy); individually packaged (sold in boxes of 10).1 boxYes30Disinfectant for floors Either locally made or commercially available (sold in 10litre containers).631Disinfectant for surfaces Ethanol based, certified for TB bacilli (if not certified, use 70% ethanol) (sold in 5litre containers).832Spray bottle For disinfectant; 1litre.633Liquid soap pH neutral (sold in 1litre bottles).1234Disinfectant for hands Ethanol-based disinfectant for hands, either locally made or commercially available (sold in 1litre bottles).1535Paper towel Single use; 150 towels per pack (sold in cartons of 30 packs).5 cartons36Cotton wool White; absorbent (bought locally by the kilogram).Purchase as required37Tissue pulp Absorbent sheets; approximately 550mm 350mm.3038If a laboratory dishwasher is available: Detergent washing powder Suitable for laboratory dishwashers (sold in 10kg containers).3639Salt Suitable for laboratory dishwashers (sold in 10kg packs).1040Rinse aid Suitable for laboratory dishwashers (sold in 1litre containers).10 Molecular biology 411.5ml standard reaction tubes Conical; with screw top and O-ring (sold in packs of 1000).10 packsYes422ml standard reaction tubes Conical; with screw top and O-ring (sold in packs of 1000).10 packsYes43Cryovials Graduated; 2ml; screw cap; vials round with an external thread; made of PP with a frosted marking area; vials sterile and able to be sterilized with -rays; stable at  196C to 121C; free from RNase, DNase, DNA and endotoxins (sold in packs of 1000).3 packs44Cryotags To fit cryovials and standard microtubes (sold in rolls of 1000). 3 rolls45PCR tubes 0.2ml; with attached caps and thin walls; free from DNase and RNase; tubes should be compatible with thermal cyclers with heated lids.Pack of 1000460.110l pipette tips Sterile and free from DNase and RNase; made of PE; with compatible filters available; 96tips per pack (sold in boxes of 10 packs).10 boxes471.020l tips Sterile and free from DNase and RNase; made of PE; with compatible filters available; 96tips per pack (sold in boxes of 10 packs).40 boxes4810100l tips Sterile and free from DNase and RNase; made of PE; with compatible filters available; 96tips per pack (sold in boxes of 10 packs).20 boxes4920200l tips Sterile and free from DNase and RNase; made of PE; with compatible filters available; 96tips per pack (sold in boxes of 10 packs).10 boxes501001000 l tips Sterile and free from DNase and RNase; made of PE; with compatible filters available; 96tips per pack (sold in boxes of 10 packs).10 boxes510.510 l tips Made of PE (sold in bulk containers of 1000 tips).10 containers521200 l tips Made of PE (sold in bulk containers of 1000 tips).5 containers531001000 l tips Made of PE (sold in bulk containers of 1000 tips).5 containers54Pasteur pipettes Sterile; single use; made of plastic; volume 1.5ml (sold in packs of 500).30 packs55Combi-tips For 5ml repeating pipette (sold in packs of 100).3 packs56Combi-tips For 10mL repeating pipette (sold in packs of 100).3 packs57Forceps For handling strips; preferably made of plastic.358Plastic bottle359Filter paper Preferably as sheets.As needed 60Special marker pen For strips (alternatively, soft-grade pencils).261Marker pen Fast-drying, black, thick-line (0.81mm) marker pens that can write on most surfaces (even when the surface is cold and wet); ink resistant to water, alcohol and autoclaving.362Nitrile gloves (size 67) Disposable; rated as CE category III (against chemical risks and resistant to ethidiumbromide, sulfuric acid or auramine staining); e"0.36mm thick.10 boxes63Nitrile gloves (size 7 8) Disposable; rated as CE category III (against chemical risks and resistant to ethidiumbromide, sulfuric acid or auramine staining); e"0.36mm thick.5 boxes64Nitrile gloves (size 8 9) Disposable; rated as CE category III (against chemical risks and resistant to ethidiumbromide, sulfuric acid or auramine staining); e"0.36mm thick.2 boxes65Liquid culture tube For MGIT.1500066Reagent cocktail For liquid enrichment.15067Reagent cocktail For minimizing contamination in liquid medium.2068Antibiotic preparation For DST in liquid medium.2069Medium base For solid culture of nonmycobacterial organisms (sold in 1kg containers).1 container70Petri dishes Either disposable or made of glass (sold in boxes of 50 pieces).24 boxes71Plastic pasteur pipettes Graduated; able to hold 1.5ml; disposable; sterile (sold in packs of 500).30 packs72Bags For noninfectious material; made of transparent PP; size 200mm 300mm (sold in packs of 100).5packs B5 Main specifications for chemicals for culture, DST and molecular biology TableB5.1 lists chemicals needed for culture, DST and molecular biology laboratories. Indicated quantities are based on experience and are suited for an NRL performing approximately 12000 cultures and about 1000FL-DST (and 100SL-DST). They should serve as an estimate quantities should be adjusted to the actual workload of the laboratory and the methods used. Table B5.1 Chemicals for culture, DST and molecular biology ItemDescription and specificationsNo. of itemsChemicals for culture of tubercle bacilliLoewensteinJensen medium1Ready-to-use medium mix From a certified supplier of Loewenstein-Jensen (LJ) medium, with correct composition of ingredients (sold in 500g containers). 72Fresh eggs From hens fed without growth factors or antibiotics, sourced from a local producer; each 1600ml of medium requires 25 eggs.20003Glycerol (C3H8O3) e"99% pure, MW 92.1g/mol (sold in 1litre bottles).24Option: Sodium pyruvate 50g (used as an alternative to glycerol for M.bovis growth).2IUTM base (called modified LJ-Medium in the standard operating procedure); made of single components5Potassium dihydrogen phosphate (KH2PO4) e"99%per analysis (p.a.); anhydrous; MW136.09g/mol.1kg6Magnesium sulfate heptahydrate (MgSO4 7H2O) e"99% p.a.; MW246.48g/mol. 500g7L-asparagine monohydrate (C4H8N2O3 H2O) e"99% p.a.; MW150.13g/mol.2 250g8Trimagnesium dicitrate nonahydrate (Mg3(C6H5O7)2 9H2O) e"95% p.a.; MW613.25g/mol (sold in 500g plastic bottles).1 bottle9Malachite green oxalate p.a. (sold by the 100g). 100g10Glycerol (C3H8O3) e"99% pure; MW 92.1g/mol (sold in 1litre bottles).2 bottles11Fresh eggs Eggs from hens fed without growth factors or antibiotics, sourced from a local producer; each 1600ml of medium requires 25 eggs.2000Ogawa medium12Sodium L-glutamate monohydrate (C5H8NNaO4 H2O) e"98% pure; MW187.13g/mol.1kg13Potassium dihydrogen phosphate (KH2PO4) e"99%p.a.; anhydrous; MW136.09g/mol.1kg14Malachite green oxalate p.a. 100g15Glycerol (C3H8O3) e"99% pure; MW 92.1g/mol.3bottles16Fresh eggs Eggs from hens fed without growth factors or antibiotics, sourced from a local producer; each 1600mL of medium requires 25 eggs.2000 Modified Ogawa medium17Sodium L-glutamate monohydrate (C5H8NNaO4 H2O) e"98% pure; MW187.13g/mol. 1kg18Potassium dihydrogen phosphate (KH2PO4) e"99%p.a.; anhydrous; MW136.09g/mol.1kg19Magnesium sulfate-heptahydrate (MgSO4 7H2O) e"99% p.a.; MW246.48g/mol. 500g20Malachite green oxalate p.a. 100g21Glycerol (C3H8O3) e"99% pure; MW 92.1g/mol (sold in 1litre bottles).2 bottles22Fresh eggs Eggs from hens fed without growth factors or antibiotics, sourced from a local producer; each 1600ml of medium requires 25 eggs.2000Phosphate buffer according to Soerensen (1/15M, pH6.8 or adjustable between pH5 and 8.2)23Disodium hydrogen phosphate (Na2HPO4) For buffer; anhydrous; p.a.; MW141.96g/mol.2 1kg24Alternative: Disodium hydrogen phosphate dodecahydrate (Na2HPO4 12H2O) For buffer; p.a.; MW358.14g/mol.2 1kg25Potassium dihydrogen phosphate (KH2PO4) e"99%p.a.; anhydrous; MW136.09g/mol. 2 2kgDecontamination proceduresPetroff method26Sodium hydroxide (NaOH) Purum e"98%; MW40g/mol; 1kg plastic bottle of pellets with tight screw cap.2 bottlesSodium phosphate method27Trisodium phosphate dodecahydrate (Na3PO4 12H2O) MW380.12g/mol.4 2.5kgNALC method28N-acetyl-L-cysteine (NALC) (C5H9NO3S) Puriss e"99%; MW163.19g/mol.250g29Trisodium citrate dihydrate (C6H5Na3O7 2H2O) MW294.10g/mol.1kg30Sodium hydroxide (NaOH) Purum e"98%; MW40g/mol; 1kg plastic bottle of pellets with tight screw cap.1bottleCetylpyridinium chloride for specimen transportation31Cetylpyridinium chloride monohydrate (C21H38ClN H2O) e"98% pure for biochemistry; MW358.01g/mol; melting point77 C.500g32Sodium chloride (NaCl) p.a. e"99%; MW 58.44g/mol.1kgPretreatment of urine for enrichment before decontamination33 5-sulfosalicylic acid dihydrate (C6H7NO3S 2H2O) p.a. e"99%; MW254.22g/mol.100gNeutralization of gastric washings34Sodium bicarbonate (NaHCO3) >99% p.a.; MW84g/mol.500gReagents for biochemical identification of M. tuberculosisParanitrobenzoic acid35Paranitrobenzoic acid (PNB, 4-nitrobenzoic acid) (C7H5O4) Purum e"98%; MW167.12g/mol.250gNiacin strips36Niacin strips (50 per pack).24 packsCatalase test37Tween 80 (polyoxyethylene-80-sorbitan monooleate)1kg38Hydrogen peroxide (H2O2) 30% p.a.; MW34.01g/mol; stabilised.250mlNitrate reduction test (nitratase test)39Potassium dihydrogen phosphate (KH2PO4) e"99%p.a.; anhydrous; MW136.09g/mol.1kg40Disodium hydrogen phosphate (Na2HPO4) Anhydrous; p.a.; for buffer; MW 141.96g/mol.1kg41Sodium nitrate (NaNO3) p.a. e"99.5%; MW84.99g/mol. 500g42Sulfanilamide (C6H8N2O2S) p.a. e"98%; MW172.21g/mol; melting point >164C.100g43N-(1-naphthyl)-ethylenediamine dihydrochloride (C12H14N2 2HCl) p.a. e"98%; MW 259.18g/mol. 25g44Zinc dust (Zn) p.a.250g45Hydrochloric acid (HCl) Purum e"35%.1litreReagents for long-term storage of mycobacterial cultures 46Skim-milk powder (Sold in 500g packs).500g47Alternative: Glycerol (Sold in 500ml quantities).4 500mlMcFarland standard48Barium chloride dihydrate (BaCl2 2H2O) e"99%p.a.; MW244.26g/mol. 250g49Sulfuric acid (H2SO4) Purum.100mlSolvent 50Dimethyl sulfoxide 99.8%, p.a.500mlDSTFirst-line drugs (of high quality with certified purity and/or potency)51Isoniazid (5g)5g52Rifampicin (1g) 1g53Ethambutol (25g)25g54Dihydrostreptomycin (5g)5gSecond-line drugs (with certified purity and/or potency)55Ofloxacin/Ciprofloxacin (2g)2g56Protionamide/ethionamide (2g) 2g57Kanamycin/amikacin (2g)2g58Capreomycin (1g)1gMolecular biologyReagent for gene amplification59Taq polymerase 250 units/vial; one vial per 200 amplifications.As neededTest kits/strips60Immunochromatographic tests For the rapid identification of M. tuberculosis.120061Line-probe assays in strips For the rapid detection of resistance to rifampicin.1000Gel electrophoresis62Agarose Universal use for separation of DNA fragments; range 100base pairs (bp) to 20kbp; melting point <90C; gel formation <45C.100g63Boric acid >99.5% for buffer.1kg64Sodium acetate trihydrate Analytical grade.1kg65Ethidium bromide1g66TRIS >99%; buffer grade. 3 1kg67EDTA 99% for biochemistry.250g68Gel load buffer 6 with Ficoll and bromophenol blue/xylencyan blue; 1 set of 6 1.8ml fractions1setDisinfectantsFumigation (BSC before filter change or emergency case)69Formalin (CH2O) Technical grade; ~37% stabilised with 10% methanol; MW30.03g/mol (sold in 1litreplastic bottles).2litresOther disinfectants (The amounts given are based on the preparation of approximately 4litres/working day, to be adjusted to needs.)70Stable chlorine disinfectant Sodium dichloroisocyanurate; 6g per tablet; 1g active chlorine per tablet; 100 tablets per pack (15 tablets per 5litres ofwarm water).5 packs71 Alternative: Calcium hypochlorite (Ca(ClO)2) Granules or tablets containing 70% available chlorine (usually requiring 5g/l) (sold in 1kg bottles).5 bottles72Alternative: Phenolic products (e.g. triclosan, chloroxylenol, orthophenylphenol); to be used at concentrations of 5% (sold in 2.5litre bottles).20 bottles73Alternative: Iodophores A combination of iodine and an inert polymer such as polyvinylpyrrolidone; to be used at concentrations of 35% (sold in 2.5litre bottles). 20bottles74Ethanol (70%) or isopropanol (2-propanol) For alcohol-based disinfectants; only 1litre/working day is required; approximately 250litres/year (sold in 5litre bottles).50 bottles B6 Detailed specifications for selected items Name of equipment: Binocular bright field microscopeCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use Microscope able to detect acid-fast bacilli (AFB), mainly in sputum smears, but also in other materials from suspected TB cases. The microscope will be used in TBmicroscopy centres, but in some settings will have additional uses (e.g. examining blood smears for malaria diagnosis). In the list below, specifications for uses other than TB diagnosis are given in italics.Main specificationsBody Microscope to have a sturdy, stable base; its focus adjustment devices to be positioned to be comfortable for prolonged use and to allow for easy, exact movements. The body to be epoxy powder coated, heat treated and resistant to standard staining reagents, disinfectants and organic solvents used to clean lenses.Optical system Colour-corrected infinity optics with parafocal distance of 45mm and tube length of 18020mm. All optical parts (including objectives, eyepieces, lenses and prisms) to have anti-reflex coating. The entire optical system (all components, including fixation) to be anti-fungus treated, with at least a 1-year warranty. Binocular eyepiece Binocular eyepiece, preferably with a tube that can be adjusted to an upper and lower position, so that it can be quickly adapted for laboratory workers of different heights. The tube to maintain parafocality while rotating through 360, at an interpupillary distance of at least 5075mm. In some Asian countries, even smaller interpupillary distances (e.g. 48mm) will be required. Viewing angle to be 30 and ergonomic.Eyepieces High-quality, paired eyepieces are required achromatic, wide field, with 10 magnification. The eyepieces to have a minimum field number of 18, and preferably 20. Both eyepieces must have dioptre adjustment for maximum acuity; no pointer is required.Objectives At least three plan achromatic objectives (10, 40, 100) are needed; objectives e"40 to be spring loaded. 10, NA: 0.25 40, NA: 0.65 50/60, NA: 0.9 (for malaria testing) 100, NA: 1.25 (for oil immersion) If not preinstalled, objectives to be delivered in transparent, break-resistant, tightly closed containers, with objectives fixed in a stable position. Marking and identification All objectives to be engraved with the following information: name or code of the manufacturer magnification and numerical aperture sign: ". 100 objective to be marked (using words or a colour code) for use with immersion oil. Nosepiece Revolving nosepiece with a backwards incline, designed to accommodate four objectives. Ports not covered by an objective to be closed with a dust-proof screw plug made of metal or hard plastic. The nosepiece to have a ribbed grip for easy rotation, a precision ball-bearing mechanism for smooth and accurate alignment, and precise stopping points. When changing from one objective to another, or rotating to the same objective, the object in the centre of the field should not appear to be displaced by more than 0.02mm in any direction in the object plane.Stage The mechanical stage to be uniformly horizontal, with sides 140mm 5mm each. The stage to have a spring-loaded slide holder, so that slides can be positioned safely and accurately, while allowing smooth travel in traverse directions. The travel range to be a least 75mm 30mm (W D); the knobs to be in a fatigue-proof position and suitable for right- or left-hand operation.Condenser Focusable Abbe-style substage condenser, 0.9/1.25, with rack and pinion arrangement incorporating an iris diaphragm. Device for blue filter (approximately 32mm) and/or other filters.Substage illuminator Modular illumination is preferable (e.g. a white light-emitting diode LED light source with a battery pack). Modules to be easy to change, preferably without tools or with a standard key that is included when delivered. The substage illuminator to have a built-in variable light source (e.g. a 2030W, 6V halogen lamp or 3W, 6V LED) with a constant power supply and automatic voltage surge protection. The light intensity to be adjustable and the on/off switch to be easily accessible. The lamp to have a lamp socket and the bulb to be easily replaceable. The housing for the light source to be designed to prevent dispersion of light and mounted to prevent heating the body of the microscope.Focusing knobs The microscope to have coaxial coarse and fine focusing knobs. The fine focusing movement to have a sensitivity of at least 500m/rotation over the entire coarse focusing range, and the total range to be at least 15mm. The focusing knobs to be positioned on both sides, and the microscope to have a safety focusing stop arrangement. All metallic parts of the microscope to be corrosion proof, acid proof and stain proof.Optional The manufacturer or supplier to provide a duly calibrated measurement instrument and demonstrate the specifications for the purchaser.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: approx. 30 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101. DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001 and a type-test certificate of relevant optical and mechanical tests.Quality and safety standards met by the product must be listed.AccessoriesDust cover, made of antistatic material.One antistatic cleaning brush for each microscope.One blue filter per microscope (wrapped separately in a box).OptionsMirror unit Plano-concave mirror, attachable to the base, for field use.Battery pack A 6 V DC battery pack with charger, that can work with a 230 V, AC, 50/60Hz electricity supply and from the 12V cigarette lighter socket in a car.Case A sturdy, lockable case for carrying or storing the microscope, with a handle and receptacles to hold accessories, objectives and eyepieces. The case to be padded and equipped with fixing points and straps to hold the microscope in place and to eliminate shocks during transportation. A set of fuses for the illumination unit to be provided, if required.If a special plug is needed for the country, a set of adapters to be provided.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each microscope, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the microscope. The manuals to include instructions for: setting up the microscope routine cleaning and maintenance (including how to change the bulb) changing the batteries inside the pack installing and using the mirror (for when the electric lamp is not working) planning periodic maintenance.Installation and maintenance The bidder to arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the microscope within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each assembled microscope to be accompanied by an authorized list of accessories and spare parts.If needed, a set of fuses for the illumination unit in use.Two spare lamps for the illumination unit (spares are not usually required for LEDs).Cleaning fluid.Packing data Packing data are not necessarily part of the bidding process, but are required for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least oneyear.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidder may propose additional products to the requirements listed above. Name of equipment: Binocular fluorescence microscopeCode number:Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The microscope will be required to detect AFB, mainly in sputum smears, but also in other materials from suspected TB cases. The microscope will be used in TBmicroscopy centres with a high workload; microscopy examination with binocular fluorescence microscopes (FMs) can be up to five times faster than with a bright field microscope, and sensitivity is up to 10% higher than with conventional microscopy. Comments: FMs with mercury lamps require a continuous power supply and a darkroom. Mercury lamps are also expensive and have a short operating lifespan (200250hours). An alternative is an FM with LEDs, which have a longer operating lifespan (1500020000hours) and do not require a darkroom.  Main specificationsBody The microscope to have a sturdy, stable base; its focus adjustment devices to be positioned to be comfortable for prolonged use and to allow easy, exact movements. The body to be epoxy powder coated, heat treated and resistant to standard staining reagents, disinfectants and organic solvents used to clean lenses.Optical system Colour-corrected infinity optics with parafocal distance of 45mm and tube length of 18020mm. All optical parts (including objectives, eyepieces, lenses and prisms) to have anti-reflex coating. The entire optical system (all components, including fixation) to be anti-fungus treated with at least a 1-year warranty.Binocular eyepiece Binocular eyepiece, preferably with a tube that can be adjusted to an upper and lower position, so that it can be quickly adapted for laboratory workers of different heights. The tube to maintain parafocality while rotating through 360, at an interpupillary distance of at least 5075mm. In some Asian countries, even smaller interpupillary distances (e.g. 48 mm) will be required. The viewing angle to be 30 and ergonomic.Eyepieces High-quality, paired eyepieces are required achromatic, wide field and with 10 magnification. The eyepieces to have a minimum field number of 18, preferably 20. Both eyepieces must have eyepiece dioptre adjustment for maximum acuity; no pointer is required.Objectives For fluorescence microscopy, at least two (preferably three) plan achromatic objectives are needed, corrected for use on slides without coverslips. Objectives e"40 to be of spring-loaded type. 10, NA: 0.25 20, NA: 0.40 (also for LED) 40, NA: 0.65 (also for LED) 50, NA: 0.80 (to replace 40) 100, NA: 1.25 (for oil immersion) If not preinstalled, objectives to be delivered in transparent, break-resistant, tightly closed containers, with objectives fixed in a stable position. Marking and identification All objectives to be engraved with the following information: name or code of the manufacturer magnification and numerical aperture sign: ". 100 objective to be marked (using words or a colour code) for use with immersion oil.Nosepiece Revolving nosepiece with a backward incline, designed to accommodate four objectives. Ports not covered by an objective to be closed with a dust-proof screw plug made of metal or hard plastic. The nosepiece to have a ribbed grip for easy rotation, a precision ball-bearing mechanism for smooth and accurate alignment, and precise stopping points. When changing from one objective to another, or rotating to the same objective, the object in the centre of the field should not appear to be displaced by more than 0.02mm in any direction in the object plane.Stage The mechanical stage to be uniformly horizontal, with sides 140mm 5mm each. The stage to have a spring-loaded slide holder, so that slides can be positioned safely and accurately, while allowing smooth travel in traverse directions. The travel range to be a least 75mm 30mm (W D); the knobs to be in a fatigue-proof position and suitable for right- or left-hand operation.Condenser Focusable Abbe-style substage condenser, 0.9/1.25, with rack and pinion arrangement incorporating an iris diaphragm.Device for blue filter (approximately 32mm) and/or other filters.Substage illuminator Modular illumination is preferable (e.g. a white LED light source with a battery pack). Modules to be easy to change, preferably without tools or with a standard key that is included when delivered. The substage illuminator to have a built-in variable light source (e.g. a 2030W, 6V halogen lamp or 3W, 6V LED) with a constant power supply and automatic voltage surge protection. The light intensity to be adjustable and the on/off switch to be easily accessible. The lamp to have a lamp socket and the bulb to be easily replaceable. The housing for the light source to be designed to prevent dispersion of light and mounted to prevent heating the body of the microscope. If a mercury lamp is used: A lamp house for a 50Wmercury lamp with mirror and lens, three-dimensional adjustment and focus. Power supply with automatic igniter for mercury lamp, including a counter to track burning hours. Set of filters, including a cut-off filter.If an LED is used: Fluorescence attachment for reflected light mode, with a blue LED light source and battery pack. Alternatively, a fluorescence attachment for transmitted light mode, with a blue LED light source to replace the light source of a bright field microscope. For each fluorescence attachment, a battery pack that can be installed between the power supply and the illumination unit (to act as a UPS and filter against voltage surge).  Focusing knobs The microscope to have coaxial coarse and fine focusing knobs. The fine focusing movement to have a sensitivity of at least 500m/rotation over the entire coarse focusing range, and the total range to be at least 15mm. The focusing knobs to be positioned on both sides, and the microscope to have a safety focusing stop arrangement.All metallic parts of the microscope to be corrosion proof, acid proof and stain proof.Optional The manufacturer or supplier to provide a duly calibrated measurement instrument and demonstrate the specifications to the purchaser.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Will depend on the type of illumination (e.g.20400W for a mercury lamp but only a few watts for an LED).Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Power supply with automatic igniter for mercury lamp with counter for burning hours. Alternative for LED: Power supply, wide-range input. Alternatively, battery pack may be used together with a charger as a UPS.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001 and a type-test certificate of relevant optical and mechanical tests.Quality and safety standards met by the product must be listed.AccessoriesDust cover, made of antistatic material.Battery pack A 6 V DC battery pack, with charger, which can work with a 230V, AC, 50/60Hz electricity supply and from the 12V cigarette lighter socket in a car.Eye protection If using an FM without a darkroom, a pair of well-fitting soft rubber pieces to protect the eyes and block stray light.Case A sturdy, lockable case for carrying or storing the microscope, with a handle and receptacles to hold accessories, objectives and eyepieces. The case to be padded and equipped with fixing points and straps to hold the microscope in place and to eliminate shocks during transportation.One antistatic cleaning brush for each microscope.At least one blue filter per microscope (wrapped separately in a box).If a special plug is needed for the country, a set of adapters to be provided.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each microscope, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the microscope. The manuals to include instructions for: setting up the microscope routine cleaning and maintenance (including how to change the bulb) changing the batteries inside the pack installing and using the mirror (for when the electric lamp is not working) planning periodic maintenance.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints, and to repair or replace the microscope within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each assembled microscope to be accompanied by an authorized list of accessories and spare parts.A set of fuses for the illumination unit in use.Two spare lamps for the illumination unit (for mercury lamps only; spares are not usually required for LEDs).Packing data Packing data are not necessarily part of the bidding process, but are required for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least oneyear.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidder may propose additional products to the requirements listed above. Name of equipment: Binocular microscope with LED fluorescence capabilityCode numberTender specificationsBidders specificationQuantity:Manufacturer:Type/model:Country of origin:Description of function and use This microscope is required for the detection of acid fast bacilli (AFB), mainly in sputum smears, but also in other clinical materials. It can be used in microscopy laboratories for the detection of AFB, and if desired for other types of microscopic examinations, either in brightfield or fluorescence microscopy (FM). Because of their high cost and lack of robustness, the classical fluorescence microscopy systems using mercury vapor lamps or other non-LED lamps are not recommended. They should only be used in laboratories performing a wide variety of tests in FM at widely differing excitation wavelengths, where LEDs would be impractical. In case only few FM applications with different excitation wavelengths are needed, modular LED FM systems with multiple or easily exchangeable LED lamps are preferred. LED-based microscopy is recommended by WHO to be phased in as an alternative for conventional ZN microscopy in both high and low-volume laboratories if the following specifications are met. Purchasers have at this moment three main options for replacement of existing microscopes with LED-FM. dual-use microscopes designed for simple switching between reflected FM and transmitted bright-field standard microscopes plus attachments consisting of special objectives and an LED light source that can adapt the microscopes for reflected FM standard microscopes plus an attachment consisting of an LED light source that is fitted onto the illumination system of the microscope to adapt it for transmitted FM.Main specificationsBody Sturdy, stable, base body with focus adjustment devices positioned for a prolonged comfortable use and easy, precise movement. The body shall be epoxy powder coated durable metal, heat treated and resistant to standard reagents used for staining, organic solvents used for cleaning of lenses and disinfectants.Optical system All optical parts including objectives, eye pieces, lenses, prisms, should have anti-reflective and anti-fungal coating with a warranty of 1 year at minimum.Binocular eyepiece Binocular eyepiece, preferably with a tube flexible for use in an upper and lower position to ease the use by different lab workers, an ergonomic viewing angle of 30, a tube rotatable through 360 at interpupillary distance ranging from at least 4875 mm, maintaining parafocality.Eyepieces Paired, high-quality, achromatic, wide field, 10x magnification. The eyepieces should have a minimum field number of 18, an eyepiece diopter adjustment on at least one eyepiece and no pointer.Objectives For fluorescence microscopy, at least two infinity-corrected Plan Achromat objectives, corrected for use without cover glass (no-cover-slip slides). Objectives e"50x should be of spring-loaded type. 10x NA: 0.25 optional 20x NA: 0.40 essential 40 63x dry: essential for confirmation 100x NA: 1.25, for oil immersion (optional) Marking and identification All objectives should be engraved with the following information: name or code of the manufacturer magnification and numerical aperture (NA).Nosepiece Revolving nose piece to accommodate at least 4 objectives, any ports not covered by an objective should be closed with dust proof metallic or hard plastic screw caps. The nose piece should be provided with ribbed grip for easy rotation on a precision ball bearing mechanism for smooth and accurate alignment with precise click stops. In changing from one objective to another or reintroducing the same objective by rotation, the object in the centre of the field should not appear displaced by more than 0.04 in the object plane in any direction.Stage Rectangular built in, uniformly horizontal, mechanical stage. The stage should be provided with a spring-loaded slide holder for safe and exact positioning of the slide. The construction should allow a smooth travel in traverse directions. Travel range of at least 75 x 30 mm (w x d), fatigue-proof position of knobs for movement, right or left-hand operation. Condenser Substage condenser of Abbe type, 0.9/1.25, with rack and pinion arrangement incorporating an iris diaphragm.Substage illuminator Substage illuminator for brightfield microscopy, with possibility to switch easily between FM and brightfield without tools with either a: built in light source (e.g. 2030 W, 6 V halogen lamp), including a constant power supply with automatic voltage recognition and surge protector. The system should provide a light intensity adjustment device, and an easily accessible on/off switch. The lamp should be provided with a lamp socket for easy replacement of the bulb. The housing for the light source should be designed to prevent dispersion of light and mounted not to heat up the body of the microscope or 3 W, 6 V white LED could be used, built-in or modular.Fluorescence illumination Fluorescence illumination, depending on microscope type may be: a built-in LED blue light source with maximum wavelength close to 450 nm for reflected light examination an LED blue light source attachment with maximum wavelength close to 450 nm that is fitted into a special objective for reflected light examination an LED blue light source attachment with maximum wavelength close to 450 nm that is fitted onto the illumination system of a standard microscope and sliding barrier 510 nm long-pass filter for transmitted light examination.Focusing knobs Co-axial coarse and fine focusing knobs capable of smooth fine focusing movement over the full range of coarse travel. The fine focusing movement should have a sensitivity of at least 500 m per rotation over the entire coarse focusing range. Focusing knobs should be at both sides. A focusing stop safety arrangement should be provided, as well as a total range of at least 15 mm.All metallic parts of the microscope to be corrosion proof, acid proof and stain proof.Electricity requirementsSupply voltage: 100230 10 V, AC, 50/60 Hz Voltage and plugs shall be adapted to those used inside the country.Power consumption: Will depend on the illumination equipment (max. 30 W)Conform to electrical safety IEC-60601-1, UL 61010-1, EN 61010-1.Power supply, wide range input with 6 V converter.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001 and a type-test certificate of relevant optical and mechanical tests.Quality and safety standards met by the product must be listed.AccessoriesDust cover, made of antistatic material.Battery pack Optional: Battery pack (6 V DC) with charger working with 100230 V AC, 5060 Hz supply, as well as from the cigarette lighter of a car, 12 V DC.Eye protection Optional: If using an FM without a darkroom, a pair of well-fitting soft rubber pieces to protect the eyes and block stray light.One antistatic cleaning brush for each microscope.At least one blue filter per microscope (wrapped separately in a box).Optional: A self-standing mirror unit adapted to the space between base and substage condenser, providing bright illumination when used in brightfield.If a special plug is needed for the country, a set of adapters is to be provided.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each microscope, written in United Nations languages (or at least in English) and preferably also in the official national language of the country requesting the microscope. The manuals to include instructions for: setting up the microscope routine cleaning and maintenance (including how to change the bulb) changing the batteries inside the pack installing and using the mirror (for when the electric lamp is not working) planning periodic maintenance.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints, and to repair or replace the microscope within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each assembled microscope to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are required for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least oneyear.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidder may propose additional products to the requirements listed above. Name of equipment: Water distiller (4l/hour)Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use Water quality determines the quality of reagents, solutions and culture media in the laboratory. Remarks Fully automated water distillers need a constant water supply of sufficient pressure (usually 3bar). If the water pressure at the entrance of the laboratory is higher than 5bar, it is to be regulated down to about 3bar. If the water pressure in the laboratory is low (less than 2bar) or fluctuating, a simple manual distiller for tap water can be used, but it will need to be monitored.Main specificationsWater distilling apparatus (with a capacity of at least 4l/hour).Wall-mounted distiller.Double-walled housing, with the outer housing made of sheet steel, protected against rust (e.g. galvanised steel, powder coated with epoxy resin).All parts in contact with steam or distilled water to be made of high-quality stainless steel.Built-in storage tank to hold around 8litres of distilled water.Cooling coil (easily accessible and exchangeable).Water supply connected through a built-in solenoid valve with sufficiently wide cross-section (e.g. inch hose).Water level regulator that switches off (i.e. stops heating and cooling the water supply) when the storage tank is full.Mechanism to automatically restart the distiller when distilled water is withdrawn.Electronic device to switch off the evaporator when impurities are detected (along with an indicator, such as an alarm or light, to show shut-down). The evaporator to have an easily accessible drainage stopcock with hose.Heating elements to be made of stainless steel.Integral filter and flow-control system.Cut-off in case of water supply shortage.Distilled water to comply with the International Pharmacopoeia, be free from pyrogen and have a conductivity of d"2.3S/cm at 20C.Net weight: approximately 20kg.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 3kW.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001.Quality and safety standards met by the product must be listed.AccessoriesElectrical conductivity meter If an electrical conductivity meter is not integrated into the distiller apparatus, an independent meter to be supplied, to be mounted at the withdraw point of distillate.Hose and clamps required to install the distiller.Additional storage tank (if larger amounts of water are required each day).Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each water distiller, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the distiller. The manuals to include instructions for: setting up the distiller routine cleaning and maintenance planning for periodic maintenance.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the distiller within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each assembled distiller to be accompanied by an authorized list of accessories and spare parts. Flexible tube and clamps (to withstand pressures up to 10bar).Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least threeyears. Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Magnetic stirrer hot plate Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use A magnetic stirrer is used to dissolve ingredients for culture media or buffer solutions.Main specificationsSturdy case coated and resistant to chemicals, dyes and disinfectants.Speed range: 601200 rpm.Heating plate Made of aluminium, stainless steel or glass ceramic; resistant to scratches and chemicals.Plate diameter: e"135mm, or (for right-angle plates) surfacee"140cm.Heat output: e"600 W.Heating temperature range: from room temperature to 250C.Heating device and temperature regulation.Safety circuit: 50C above hot-plate temperature.Temperature setting.Temperature accuracy at hot plate: 5C.Load on plate: up to 20kg.Permissible ambient temperature: 540C.Permissible relative humidity: d"80%.Weight: <5kg.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 800 W.Conform to electrical safety standards IEC 60601 1, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001.Quality and safety standards met by the product must be listed.AccessoriesSet of 10 magnetic bars: 1נ15mm, 2נ20mm, 2 נ25mm, 1נ30mm, 2נ40mm, 2נ50mm.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each hot plate, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the hot plate. Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the hot plate within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each magnetic stirrer hot plate to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least one year.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Precision balanceCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use A precision balance is mainly used to weigh dyes for staining solutions and components for culture media. Temperature variation and static electricity will cause precision balances to display erratic readings. Therefore, the balance is to be operated on an antistatic surface, in a room with a constant temperature and a steady relative humidity of >65%.Main specificationsElectronic balance, weighing capacity 60g, 120g or 210g.Tare range=full capacity by subtraction.Stabilization time: d"5seconds.Housing resistant to chemicals and cleaning materials.Stainless steel weighing pan, approximately 115mm diameter.Adjustable feet (so the balance can be levelled).Waterproof display and keypad, sealed by a durable, flexible membrane.Background illuminated (backlit) display with digits at least 15mm high.User-friendly menu (preferably in different languages but at least in English) so the balance can be configured to individual requirements.Level indicator to be close to the display or in the view field of the display.Built-in motorized calibration of weight with automatic adjustment.Readability: 0.001g (1mg).Repeatability: 0.001g (1mg).Linearity: 0.002g (2mg).Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet country requirements.Power consumption: Low.Optional: Battery with a rechargeable battery pack and main power DC transformer.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the balance has been calibrated at the factory.Quality and safety standards met by the product must be listed.AccessoriesSet of calibration weights.Protective dust cover.Weighing scoop, 90mm, stainless steel.Operation and maintenance manual At least one set of operation, maintenance and service manuals written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the precision balance.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the balance within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication materials, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each balance to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Five years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: pH meterCode number:Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use A pH meter is required to check the pH of buffer solutions and/or culture media solutions used in a general diagnostic TB laboratory.Main specificationsPortable pH meter with waterproof housing (according to IngressProtection 67).Resolution: d"0.01 pH units.Accuracy: 0.01 pH units.Temperature measurement: 0.1 C accuracy.Calibration with at least three standard calibration buffers (pH4.0, 7.0, 10.0); automatic calibration preferred.Calibration reminder: adjustable from 1 to 999days.Temperature compensation, preferably automatic.Illuminated display with indication after stabilisation of measured values.Multi-function display.OptionsAbility to store calibration data (day, month, year, zero point and two values for steepness of curve).Complete good laboratory practice (GLP) compliance record.Ability to store e"500 measurements, automatically logged.Password-protected data access. Transport case, with additional compartments for a glass combination pH electrode with integrated temperature sensor, electrode holder swing arm and calibration buffers.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Low.Optional: Battery with a rechargeable battery pack and main power DC transformer.DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. A certificate to state that the pH meter has been calibrated at the factory.Quality and safety standards met by the product must be listed.AccessoriesOptional: RS 232 interface cable for connecting the pH meter to a computer.Optional: Software, preferably for installation under Windows. Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the pH meter.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the pH meter within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication materials, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each pH meter to be accompanied by an authorized list of accessories and spare parts.Supply of stock of calibration buffers.Optional: Electrode.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Two years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Analytical balanceCode number:Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use An analytical balance is needed to prepare media containing drugs, for DST. The balance may also be used to calibrate, recalibrate and maintain microlitre pipettes used in the laboratory, especially for molecular biology. Temperature variation and static electricity will cause analytical balances to display erratic readings. Therefore, the balance is to be operated on an antistatic surface, in a room with a constant temperature and a steady relative humidity of >65%.Main specificationsWeighing capacity: 60200g.Tare range=full capacity by subtraction.Stabilization time: d"5seconds.Housing resistant to chemicals and cleaning materials.Glass doors (not plastic) that close tightly.Stainless steel weighing pan, approximately 80mm diameter.Full glass windscreen, able to be opened on both sides and from the top cover. Adjustable feet (so the balance can be levelled).Waterproof display and keypad, sealed by a durable flexible membrane.Background illuminated (backlit) display with digits at least 15mm high.User-friendly menu (preferably in different languages but at least in English) so the balance can be configured to individual requirements.Level indicator to be close to the display or in the view field of the display.Built-in motorized calibration of weight with automatic adjustment (or calibration using an external standard weight).Readability: 0.0001g (0.1mg).Repeatability: 0.0001g (0.1mg).Linearity: 0.0002g (0.2mg).Optional: Bidirectional RS 232C interface with ISO/GLP-compliant data export.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements. Power consumption: Low.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. The manufacturer to provide a declaration of conformity to standards that apply to the product, including ingress protection rating and weight classifications and applications. One certificate to state that the balance has been calibrated at the factory. Certificates to be provided for each item supplied. Quality and safety standards met by the product must be listed.AccessoriesBalance table with vibration bumpers, preferably granite isolator.Protective dust cover.Set of calibration weights.Optional: Weighing scoop, 90mm, stainless steel.Optional: RS 232 interface cable.Operation, installation and maintenanceInstallation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel at the place indicated, free of cost. Detailed installation prerequisites to be communicated to the purchaser in advance, especially for the electric power supply needed, including type of plug (or other way of connection). Detailed instruction of laboratory personnel on use, function and maintenance of the equipment (user training), as well as a comprehensive maintenance plan (logbook with daily, weekly, monthly and quarterly maintenance checklist), to be provided. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide a functioning after-sale service covering the whole country. The service to have adequate infrastructure, competent staff and sufficient spare parts to be able to respond to any complaints and to repair or replace the balance within 14days. Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication materials, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each balance to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least three years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Gas burner for biological safety cabinet Code number:Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use A safety gas burner allows flame control with ignition on demand and is suited for work in a BSC.Main specificationsCompact design (W H D, max.130mm 60mm 170mm); stainless steel and glass with fire-proof controls; for use in a BSC. Nozzles for the use of natural and propane/butane gas.Gas input filter to protect magnetic valve.Working pressure for natural gas: 1825 mbar.Working pressure for propane or butane: 2050 mbar.Operation mode: both hands free, with foot switch, automatic ignition.Two modes: conventional short term (flame during pressed foot on switch) and flexible startstop function with timer.Stable flame in strong laminar air flow.Ignition and flame control.Automatic cut-off of gas supply when re-ignition fails for more than 15seconds.Alarm: audible, with display on dysfunction. Overheating protection.Integrated drains for liquids accidentally spilled into the burner head.Removable burner heat.Adjustable burn time (1 sec  e"60min).Tilting of the instrument to both sides possible.Electricity requirementsSupply voltage: 230 10 V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 50 W.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. Certificates to be provided for each item supplied.Quality and safety standards met by the product must be listed.AccessoriesGlass spatter guard: a glass cylinder fitted to surround the flame for protection against spattering infectious material.Adapter for the connection of gas cartridge (e.g. CV 360 or C 206 including pressure-reducing valve and gas safety hose of 500mm).Gas safety hose with safety clamps on both sides, 7501000mm long.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the gas burner.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the gas burner within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including tools to maintain and change nozzles, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each safety burner to be accompanied by an authorized list of accessories and spare parts.Nozzle according to the gas used.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Two years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Micro-incinerator Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The micro-incinerator allows sterilization of metal inoculating loops without a flame and is suited for work in a BSC.Main specificationsHeating element of ceramic surrounded by isolating cover.Quick infrared heating to temperatures e"800 C for fast sterilization.Stand with suction-cup feet (or equivalent) for stable, safe operation.Possibility to fix the incinerator to a stand at different angles.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz, and appropriate DC power supply. Voltage and plugs to be adapted to meet the country requirements.Power consumption: 2000 W.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. Certificates to be provided for each item supplied.Quality and safety standards met by the product must be listed.AccessoriesAttached loop holder if desired.Operation, installation and maintenanceOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the micro-incinerator.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the micro-incinerator within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each micro-incinerator to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Twoyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Biological safety cabinet class I Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use This BSC is used in a TB laboratory for processing specimens consisting of liquefaction of sputa and handling cultures of tubercle bacilli. This type of BSC is not adequate for handling volatile or toxic chemicals or radionuclides. Before ordering a BSC, facility and engineering requirements to be checked. Organization of a periodical recertification of the BSC by an authorized agency to be in place.Main specificationsThe BSC to meet the requirements of EN 12469 or NSF 49.External height d"2200mm including support stand, allowing an available space of at least 400mm from the top of the BSC to the ceiling. Higher versions may be accepted, provided the 400mm over the BSC is available to measure air velocity above the exhaust filter and to have enough space for changing the filter and for ducting and/or a thimble connection to outlet.Internal working area (approximate): For a BSC of 120cm (4ft): width 1150mm depth 630mm height 650750mm. For a BSC of 150cm (5ft): width 1450mm depth 630mm height 650750mm. For a BSC of 180cm (6ft): width 1700mm depth 630mm height 650750mm. A BSC of 120cm (4ft) provides the minimal space needed for safe work.Inside finish: stainless steel, high quality (e.g. grade 304).External housing, including screws, made of stainless steel or equivalent resistant galvanized (zinc-coated) sheet steel, subsequently powder coated and thermally hardened; minimum 80m thick, or other material that is hard-wearing, resistant to disinfectants and chemicals used in a TB laboratory, and abrasion resistant. Vertical window: aerosol-tight, sliding, safety glass (laminated multilayer safety glass only), thickness e"6.7mm. Foldout windows made of tempered glass are also acceptable. Acrylic glass is not acceptable.Working aperture: e"170mm measured from work surface to the bottom of the window.Maximal lifting height of front window: 500mm.Ability to lock the window hermetically for gaseous disinfection for filter decontamination.Single-piece working surface preferred. Alternatively: Working surface as segments.Noise pressure level: d"60 dbA.Internal fittingsOptional (if a safety gas burner will be used): Gas tap with solenoid valve, optional right or left side. For a laboratory located in a seismic area, gas pipes are not recommended; small gas containers (approximately 200400ml) with butane gas directly fixed to the burner to be used instead. Not necessary when a micro-incinerator is used.Two plugs, 230 10V, AC, 50/60Hz, protected with separate T5A (slow blow) fuse. Voltage and plugs adapted to those used in the country. Warning: Plugs inside the BSC may differ from the main connection to the electricity network.Flicker-free, low-glare, warm-coloured light, >1000 lux.Optional: UVC light (253.7 nm wavelength); 30 W with hour counter; with interlock with white light so that the UVC light can be switched on only when the white light source is switched off.Control display on the front of the BSC.Electrical control or indicators. Electronic fan control.Flow meter for air influx velocity.Operating hours indicator (counter).Optional: UV light timer.Filter and flow conditionsPrefilter construction preferred; easily accessible filter change without tools preferred.HEPA filter (exhaust air filter); classification at least H14; conforming with EN 1822; metal framed. Inward air velocity: e"0.38m/s.Exhaust volume airflow/ inward volume airflow: 300600m/h. Blower system to be able to maintain the airflow within a minimum window (narrow limits) on voltage fluctuations. Data to be available on request.Alarms, visible or audible, for any unsafe condition of the BSC (e.g.airflow, window position). Possibility to shut down alarm for cleaning and maintenance.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Lead fuse T16A (slow blow) or circuit breaker B16. The electrical regulations valid in the country of use as well as the relevant connection conditions are required.Power consumption (approximate): For a BSC of 120cm (4ft): 600 W. For a BSC of 150cm (5ft): 800 W. For a BSC of 180cm (6ft): 1000 W. Power consumption for plugs inside: Approximately 1000 W. Note: In areas with frequent breakdown of electricity, BSCs with low energy consumption can be of advantage. A UPS with lower capacity can be used. Modify according to the BSC dimensions.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. The manufacturer to individually test each BSC before shipment. The test report to be provided to the customer, with a duplicate fixed to the BSC. Tests to be performed with research-grade instruments for valid calibration: inflow air velocity filter leak scan for HEPA filter to document filters efficiency and integrity operator protection by discus test method specified in EN12469.Quality and safety standards met by the product must be listed.AccessoriesTable or support frame (support stand) for a working height 782cm, adjustable at least at three feet (points) to level. A telescopic support stand is advisable for a flexible use.Ergonomic laboratory chair, designed for infectious laboratory areas: adjustable height to suit different users, seat range approximately 400490mm adjustable-angle back rest (no arm rest) caster wheels (five) all metal parts chrome plated disinfectable with alcohol-containing disinfectants. Air duct construction to hard duct out or thimble exhaust air from the BSC. The air duct to be made for the BSC offered and to fit precisely. All standard accessories, consumables and parts required for the proper installation, operation and maintenance of the BSC to be included in the offer by the supplier, and to be specified and quantified. Operation, installation and maintenanceOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the BSC.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the BSC within 14days.Initial on-site testing (inward airflow velocity and aerosol leak test for the exhaust air filter) to be carried out and documented by a certified expert. Measurement results to be printed out for documentation in the maintenance record. Warranty starts with certification on site.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning equipment, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above). If special tools are needed (e.g. to change the filters), they must be provided.Spare parts Each assembled BSC to be accompanied by an authorized list of accessories and spare parts.At least one, and preferably two, additional sets of HEPA filter as specified above .Warning: Special clamps may be needed to fix HEPA filter.Three prefilters.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Three years, except for filters and UV lamp.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Biological safety cabinet class II Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function The class II BSC is used in a TB laboratory for processing specimens consisting of liquefaction of sputa and handling cultures of tubercle bacilli. This type of BSC is not adequate for handling volatile or toxic chemicals or radionuclides. Before ordering a BSC, facility and engineering requirements to be checked. Organization of a periodical recertification of the BSC by an authorized agency to be in place. Main specificationsThe BSC to meet the requirements of classIIA2 NSF 49 or classII EN 12469; specifically, with regard to inward airflow (e"0.40m/s according to EN 12469:2000 or e"0.50m/s according to NSF 49:2004).External height d"2200mm including support stand, allowing an available space of at least 400mm from the top of the BSC to the ceiling. Higher versions may be accepted, provided the 400mm over the BSC is available to measure air velocity above the exhaust filter, and to have enough space for changing the filter and for ducting and/or a thimble connection to outlet.Internal working area (approximate): For a BSC of 120cm (4ft): width 1150mm depth 630mm height 650750mm. For a BSC of 150cm (5ft): width 1450mm depth 630mm height 650750mm. For a BSC of 180cm (6ft): width 1700mm depth 630mm height 650750mm. A BSC of 120cm (4ft) provides the minimal space needed for safe work.Inside finish: stainless steel, high quality (e.g. grade 304).External housing, including screws, made of stainless steel or equivalent resistant galvanized (zinc-coated) sheet steel, subsequently powder coated and thermally hardened; minimum 80m thick, or other material that is hard-wearing, resistant to disinfectants and chemicals used in a TB laboratory, and abrasion resistant. Vertically adjustable sliding window: aerosol-tight, sliding, safety glass (laminated multilayer safety glass only), thickness e"6.7mm, counterbalanced.High optical transmission, but absorption of UV light; minimal reflection.Working aperture: e"170mm measured from work surface to the bottom of the sash window. Maximal lifting height of front window: 500mm.Ability to lock the window hermetically for gaseous disinfection for filter decontamination.Single-piece working surface with integrated (V-shaped) front air grill. Alternative: Working surface as segments.Noise pressure level: d"60 dbA.Internal fittingsOptional (if a safety gas burner will be used): Gas tap with solenoid valve, optional right or left side. For a laboratory located in a seismic area, gas pipes are not recommended; small gas containers (approximately 200 400ml) with butane gas directly fixed to the burner to be used instead. Not necessary when a micro-incinerator is used.Two plugs, 23010V, AC, 50Hz, protected with separate T5A (slow blow) fuse. Voltage and plugs adapted to those used in the country. Warning: Plugs inside the BSC may differ from the main connection to the electricity network.Flicker-free, low-glare, warm-coloured light, >1000 lux.Optional: UVC light (253.7 nm wavelength); 30 W with hour counter; with interlock with white light so that the UVC light can be switched on only when the white light source is switched off.Control display on the front of the BSC.Electrical control or indicators.Electronic fan control. Flow meter for air inflow velocity.Flow indicator or meter for air downflow velocity.Operating hours indicator (counter).Optional: UV light timer.Filter and flow conditions. Prefilter construction preferred; easily accessible, filter change without tools preferred.HEPA filter (exhaust air filter); classification at least H14; conforming with EN 1822; metal framed.Air downflow velocity: NSF 492002: Requires compliance with the manufacturers set points, or downflow velocity with a deviation of <0.025m/s from a nominal set point. EN 12469: Airflow velocity should be between 0.25 and 0.50m/s and is defined by the manufacturer according to the construction. Additionally, no individual measurement should differ by more than 20% of the value requested by the manufacturer within the limits given.Air circulation volume flow: For a BSC of 120cm (4ft): 7001200m/h. For a BSC of 150cm (5ft): 10001500m/h. For a BSC of 180cm (6ft): 12001900m/h.Modify according to the BSC dimensions.Influx air velocity: According to NSF 49, the average airflow velocity at front aperture should be 0.51m/s for class A2. EN 12469 does not subclassify within class II BSC. The average airflow velocity at front aperture should be at least 0.4m/s, according to the manufacturers specifications. Exhaust volume airflow/fresh airflow inward: For a BSC of 120cm (4ft): 300600m/h. For a BSC of 150cm (5ft): 400700m/h. For a BSC of 180cm (6ft): 500900m/h.Modify according to the BSC dimensions.Blower system to be able to maintain the airflow within a minimum window (narrow limits) on voltage fluctuations. Data to be available on request. Alarms, visible and/or audible, for any unsafe condition of the BSC (e.g. airflow, window position, hardware or software errors). Possibility to shut down alarm for cleaning and maintenance.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Lead fuse T16A (slow blow) or circuit breaker B16. The electrical regulations valid in the country of use as well as the relevant connection conditions are required.Power consumption (approximate): For a BSC of 120cm (4ft): 600 W. For a BSC of 150cm (5ft): 800 W. For a BSC of 180cm (6ft): 1000 W. Power consumption for plugs inside: Approximately 1000 W. Note: In areas with frequent breakdown of electricity supply, BSCs with low energy consumption can be an advantage; a UPS with lower capacity can be used.Modify according to the BSC dimensions.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. The manufacturer to individually test each BSC before shipment. The test report to be provided to the customer, with a duplicate fixed to the BSC. The tests to be performed with research-grade instruments for valid calibration according to test methods outlined in EN 12469 or NSF 49. The test report to contain at least data on: inflow air velocity downflow air velocity filter leak scan for both filters to document filters efficiency and integrity.Quality and safety standards met by the product to be listed.AccessoriesTable or support frame (support stand) for a working height of 782cm, adjustable at least at three points (feet) to level. A telescopic support stand is advisable for a flexible use.Ergonomic laboratory chair, designed for infectious laboratory areas: adjustable height to suit different users, seat range approximately 400490mm adjustable-angle back rest (no arm rest) caster wheels (five) all metal parts chrome plated disinfectable with alcohol-containing disinfectants.Air duct construction to hard duct out or thimble exhaust air from the BSC. The air duct to be made for the BSC offered and fit precisely. Depending on the ventilation system for the containment room, a motorised flap in the hood and a trigger for the external ventilator or equivalent regulatory device may be needed.All standard accessories, consumables and parts required for the proper installation, operation and maintenance of the BSC to be included in the offer by the supplier and to be specified and quantified.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the BSC.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the BSC within 14days.Initial on-site testing (aerosol leak test, recirculating air filter, exhaust air filter, airflow measurements inside the BSC and inward/exhaust airflow) to be carried out by a certified expert. Measurement results to be printed out for documentation in the maintenance record. Warranty starts with certification on site.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning equipment, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above). If special tools are needed (e.g. to change filters), they must be provided.Spare parts Each assembled BSC to be accompanied by an authorized list of accessories and spare parts.At least one, and preferably two, additional sets of HEPA filters as specified above.Warning: Special clamps may be needed to fix HEPA filter.Three prefilters.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Three years, except for filters and (UV) lamp.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Uninterrupted power supply with battery pack for BSC Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The UPS must be used in any settings that have frequent problems in the electricity network (e.g. surges, sags, spikes and blackouts) to assure and back up the function of the BSC, so that any current work can be finalized and all potentially infectious sources closed. If the BSC is connected to a generator, the UPS will maintain the function of the BSC during the time needed for the generator to start and to provide full power.Main specificationsUPS: microprocessor controlled, line interactive, online continuous transducer, 20minutes.Booster function to regulate up voltage breakdown to 170 V.Buck function to regulate down voltage increase up to 280 V.Filter to protect against voltage spikes.Protection against overload and short circuit.Advanced battery check for automated periodic battery inspection. Indicators for status (e.g. normal function, net down, working on battery, loading battery, battery capacity).Sleep mode if item consuming power is shut off.Power: 230 V 25%, 50Hz or 60Hz (10%) with automatic recognition.Battery: maintenance-free, automatic shut-off before reaching the level of discharge from which recharging to the original capacity will no longer be possible.Time for recharging: approximately 4hours to reach at least 90% of total capacity.Outlet voltage: 230V 3%, 50 or 60Hz 0.5% (if the countrys standard voltage is 110 V AC, adjustment will be needed).Changeover time: <5msec.Efficiency coefficient: approximately 98%, on battery >85%.Noise at 1m distance <48 dBA.Permissible ambient temperature and relative humidity: 040C and95% (not condensing).Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 1500W (depending on the model chosen).Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001.Quality and safety standards met by the product to be listed.AccessoriesBattery pack.Connection (cable and fittings) for battery pack.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the UPS.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each UPS to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least two years for UPS; at least fiveyears for battery pack. Name of equipment: Mini-shaker/vortexCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The mini-shaker is for use in a BSC. Main specificationsSturdy housing; metal; coated; disinfectable.Design feature: flat construction.Strong orbital movements.Rotation speed: 02500 rpm. Orbit: e"4mm.Standing on four rubber feet designed to prevent sliding of the instrument during shaking.Two operating modes: continuous and touch function.Head: about 20mm.Slip-resistant stand.Overheating protection.Permissible ambient temperature and relative humidity: 5 40C and d"80%.Weight: 2.5 5kg.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: 50 100 W.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001.Quality and safety standards met by the product to be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each mini-shaker, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the mini-shaker.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each mini-shaker to be accompanied by an authorized list of accessories and spare parts. Fuses.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Two years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchaserss country; the equipment may need to withstand high humidity, fungi, thunderstorms and spikes in the electricity supply. Appropriate packaging to be provided for longer storage. Bidders may propose products additional to the requirements listed above. Name of equipment: Incubator Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The incubator is used to allow growth of TB bacilli on culture media.Main specificationsChamber made of stainless steel, easy to clean.Housing: corrosion resistant, made of either stainless steel or zinc galvanized sheet metal coated with epoxy, hardened by heat treatment. Double doors, double lock.Inner doors: security glass. Volume of interior housing: e"600litres, corresponding to a capacity of about 2000 2200 TB culture tubes.Designed specially for temperatures up to 70C, optimised for living cultures at 37C.Temperature variation at 37C: d"0.1C.Temperature overshoot within this limit.Temperature range: 30C (at least 5C above ambient temperature) to 70C.Adjustable over-temperature protection controller  TWW protection class 3.1; or electronic temperature limiter  TWB protection class 2.Uniformity of temperature inside the chamber (deviationd"0.2C). Two high-grade temperature sensors (class A) with mutual monitoring and taking over performance at same working temperature.Integrated programmable timer.Programs stored on power failure.Adjustable air flap for preheated fresh air intake.Vent connection with restrictor flap.Display of temperature.Alarm: audible, with display on dysfunction.Function signals for operating mode.Optional: Long-term logging (ring store) internal memory to save temperature and error states, with time stamp to the minute.Optional: Serial RS 232 interface with software for readout of data with PCL3-compatible printer.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 2000 W.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the incubator has been calibrated at the factory to 37C.Quality and safety standards met by the product to be listed.AccessoriesOne or two additional perforated stainless steel shelves, nontipping, to match four shelves per incubator.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the incubator.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the incubator within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning equipment, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each incubator to be accompanied by an authorized list of accessories and spare parts.A set of fuses.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Twoyears for regulator; fiveyears for heating coils.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: CentrifugeCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The centrifuge will be used in a TB laboratory to spin down mycobacteria in liquefied, decontaminated materials. The compartments containing the centrifuge tubes (buckets) need to be absolutely aerosol tight and closed by transparent lids. At least 3000G will be required for efficient sedimentation within 20minutes of centrifugation. The centrifuge should preferably be a desk-top one; stand-alone models are also suitable, but are more expensive.Main specificationsMetal housing, chamber stainless steel.Cooling capacity at maximum speed at +4C.Standby cooling.Induction motor, brushless drive.Rotor with swing-out buckets, at least four positions.Rotor buckets with aerosol-tight, transparent, clipping lids.Inserts for buckets adapted to 50ml centrifuge tubes, conical.Inserts for buckets adapted to 15ml centrifuge tubes, conical.Capacity: about 20 50ml.Maximum revolutions per minute: approximately 4500 rpm, corresponding to a radius of approximately 15cm.Relative centrifugal force (rcf): approximately 3400G.Noise level at maximum speed: not more than 60 dbA.Programmable for all parameters (switchable between rpm and rcf) of a run; large display.Soft start and different acceleration levels (19); different braking levels (19) and brake force cut-off.Input and recall of programs; at least 20 storage positions.LCD display (protected against splash of liquids) for indication of run time, speed (rpm) or rcf (after entering centrifugation radius) switchable, actual temperature, time left to finish run.Imbalance switch-off.Motor overheating protection.Chamber overheating protection.Rotor recognition for appropriate over-speed protection.Safety lock of lid during run and as long as the rotor is moving.Possibility of mechanical opening of lid if there is current blackout.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 1800 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Excess-voltage category II.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the centrifuge has been calibrated at the factory.Quality and safety standards met by the product to be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the centrifuge.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the centrifuge within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning equipment, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each centrifuge to be accompanied by an authorized list of accessories and spare parts.Device for mechanical opening of the centrifuge after automatic blocking as consequence of currency blackout.Lubricants for movable parts and gaskets.Spare fuses.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Threeyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Autoclave Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use Autoclaves are used for sterilization of infectious or clean materials. Remarks Many countries have very strict regulations on the construction, use, maintenance and location of autoclaves within the laboratory department. For sterilization of infectious waste, some countries allow autoclaves with fractionated prevacuum or at least pulsed heat-up (over-pressure pulses) only.Main specificationsVertical autoclave, universal basic version for microbiological standard laboratory to sterilize liquids, instruments, glassware, plastic articles or general infectious waste.Single-wall construction; chamber, door, doorframe, bolts made of corrosion-resistant material and able to prevent stress cracking.Pressure vessel compliant with international standards.Chamber volume: e"70litres.Heating device (steam generator) horizontally mounted, preferably separated from the chamber with minimal water volume (4 7litres).Air removed by upward displacement.Automatic water feed; connection to a demineralised water supply.Integrated pump to equalize pressure variations in external supply lines.Automatic level control before, during and after the sterilization cycle.Low-water-level cut-out device.Fast safety lid lock.Lid lock by a circumferential, durably heat- and pressure-resistant seal.Control lock-out switch that prevents starting a cycle if the door is not locked safely.Control that prevents opening the door until chamber is depressurized.Temperature-dependent door-locking system according to international standard.Maximum operating pressure: 2.5 bar. Maximum operating temperature: 134C (273 F).Sterilisation timer: 1250minutes.Instrument sterilization timer: up to 72hours.Timer-controlled sterilization of load at night.A visual chamber gauge, which easily identifies pressure in the chamber, must be accessible to the operator as a backup to the control readout when no electrical power is available.Microcomputer control system. The control panel to be mounted so that the components sensitive to steam and heat are protected.Large LCD display showing: temperature steam pressure sterilization time stage of cycle alarm information.Preselection of languages for menu-presented instructions, at least including English.Protected keyboard with acoustic confirmation signal.An access code to prevent programming changes of cycle parameters by unauthorized persons. A key lock is not recommended.Presetting of at least four programs: two for liquids, one for solids, one for waste. Load capacity: approximately 25kg of waste or 25kg of solids or 20litres of liquid.Batch documentation: batch number, date, temperature, pressure and sterilization phase; built-in printer satisfying GLP and standard operating procedure (SOP) requirements, and/or storage device (RS 232).Exhaust air filtration with condensate sterilization for emission-free sterilization of infectious pathogens; equipped with filter cartridge of 0.2m pore size, with easy access for replacement. Autoclave equipped for prevacuum, gravity and flash cycles.For autoclaves used in media kitchen: dual, built-in temperature probes (e.g. two PT-100 sensors), one flexible for accurately monitoring sample temperatures to ensure appropriate media or buffer sterilization device for rapid cooling.Safety valves; over-pressure relief valve.Low-water-level interrupt.Over-temperature and over-pressure protection limiter.Lid interlock.Alarm: audible, with display on dysfunction.All information on alarm to be in full writing and not based on a code. Even with a total control failure, all mechanical safety features must be left intact.RS 232 interface for direct connection to a personal computer (PC), and programs for conforming documentation, diagrams, storage, printout.Optional: A manual control that can run a complete cycle manually in case of system failure.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz; plug type adapted to the country or three-phase 380 V, 50Hz. Power consumption: 3kW or 4.5kW. 16A fuses.Re-setable over-current breaker fitted for protection.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the autoclave has been calibrated at the factory.Certificates for design and safety regulations.Quality and safety standards met by the product to be listed.AccessoriesSteel stand, appropriately coated for corrosion protection or made of stainless steel.Optional: In areas with possible earthquakes, this stand to be designed to meet the seismic requirements according to the zone assignment. Three stainless steel wire baskets; diameter and height adjusted so that two fit into the autoclave at the same time.Two stainless steel wire baskets; diameter and height adjusted so that one fits into the autoclave.Three stainless steel buckets with lid; diameter and height adjusted so that two fit into the autoclave at the same time (usually 380mm 290mm).Two stainless steel buckets with lid; diameter and height adjusted so that one fits into the autoclave.Chemical indicator tape for sterilization.Biological indicator.Tool to open the autoclave in case of electricity breakdown (if needed).Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the autoclave.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation should be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the autoclave within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each autoclave to be accompanied by an authorized list of accessories and spare parts.Set of autoclave internal fuses (if any).Sealing ring for lid.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: For pressure vessel and valves, at least fiveyears; for heating elements, at least threeyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Hot-air oven Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The hot-air oven is used to dry and sterilize glassware and small laboratory materials after cleaning and before reuse.Main specificationsChamber made of stainless steel, easy to clean.Housing: preferably stainless steel or at least with a durable, corrosion-resistant coating of metal (e.g. galvanized sheet metal coated with epoxy, hardened by heat treatment). Volume of interior housing: e"250litres for regional/reference laboratories, 125litres for other laboratories.Two doors, double lock.Temperature range: from 10C above ambient to +250C.Forced air ventilation by air fan.Air fan: <58dB at full speed; speed adjustable.Adjustable air flap for preheated fresh air intake.Vent connection with restrictor flap.Alarm: audible, with display on dysfunction.Switch-off function at approximately 10C above the temperature set.Digitally adjustable electronic controller, TWW protection class 3.1.Mechanical temperature limiter at fixed value 10C above maximal hot-air oven temperature (250C).Integrated programmable timer.Programs stored on power failure.Display of temperature.Function signals for operating mode. Optional: Serial RS 232 interface with software for readout of data with PCL3-compatible printer.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: 2400 W (during heating); adaptation of heating power according to the preset temperature value.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the hot-air oven has been calibrated at the factory for +160C. Quality and safety standards met by the product to be listed.AccessoriesOne additional set of perforated stainless steel shelves, nontipping, if less than two are provided with the hot-air oven.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the oven.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the oven within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each hot-air oven to be accompanied by an authorized list of accessories and spare parts. A set of fuses, if used inside the instrument for regulation.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Threeyears for regulation; fiveyears for heating coils.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Thermal anemometerCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use An anemometer is a device for measuring the velocity of air or gas flow. In a TB laboratory, an anemometer is used to determine the airflow into, out of and inside a BSC, in order to test the flow functions of a BSC.Main specificationsSturdy and easily cleaned instrument case.Foil-protected keyboard.Instrument with batteries (freely movable and independent of external electricity supply).Low-battery condition indicated on display.Small sensor (<15mm) attached with a telescopic probe tip (extension to about 1 m length), suited also for measurements in small ducts.Indicator at the probe and at the lower end of the telescope for optimal alignment to the direction of the measured airflow.Suitable cap for sensor to ensure zero adjustment.Wide temperature range, at least 070C.Indication of velocity and temperature.Compensation for variations in airstream temperature by a second thermistor that simultaneously senses the actual airstream temperature. Different velocity ranges; at least one of 02m/s.Real-time display of velocities.Memory containing about 99 locations.Storage of current velocity and temperature on demand.Indication for memory full.Storage functions to display average, maximal and minimal values of velocity or temperature on recall.Resolution of velocity reading: 0.01m/s.Resolution of temperature reading: 0.1C. Electricity requirementsSupply voltage: BatteryDocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the anemometer has been calibrated at the factory.Quality and safety standards met by the product to be listed.AccessoriesOptional: Data logger as an extension and software for processing the data on a PC.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the anemometer.Maintenance The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the anemometer within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each anemometer to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Three years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Appropriate packaging should be provided for long-term storage. Bidders may propose products additional to the requirements listed above. Name of equipment: InspissatorCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The inspissator is required for the preparation of egg-based media. Main specificationsHousing and chamber made of polished stainless steel.Temperature range: 2585CMaximal temperature: 95C.Working temperature: 85C.Temperature deviation: 0.75C.Automatic temperature control and regulation.Overheating protection and automatic shut-off.Water level sensor. (Instruments with hot air, ventilated or not, through the chamber are not recommended.)Automatic shut-off on lowered water level.Electronic timer, 06hours.Capacity: >350 tubes (1618mm in diameter, 125160mm in length) or universal bottles. Racks for tubes in slant position; angle adjustable; covering full capacity of the instrument.Alarm: audible, with display on dysfunction.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: 6003000 W.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the inspissator has been calibrated at the factory.Quality and safety standards met by the product to be listed.AccessoriesOptional: Inserts for universal bottles.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each inspissator, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the inspissator.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the inspissator within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each inspissator to be accompanied by an authorized list of accessories and spare parts.One additional set of racks covering full capacity of the instrument for a second batch.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Fiveyears for the heating coil; at least one year for the electronics.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Refrigerator with freezer compartment Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use Only for use with water solutions.Main specificationsEnergy classification A.100% CFC- and HFC-free.Total usable capacity: 300 20litres.Refrigeration compartment optimally regulated at 48C.Separate freezing compartment, freezing to at least 22C; volume 50 10litres. Optional: A refrigerator without freezing compartment may be used. The size of the refrigerator needs to be adjusted to the requirements of the laboratory.Temperature control settings and gauges in each compartment.Automatic defrosting.Four shelves of safety glass.Humidity regulation.Variable door liner arrangement. Right-hand mounted door, reversible.Usable up to 40C ambient temperature.Adjustable feet for levelling.Fungus-resistant door gasket.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately150 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101. Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. Declaration of conformity to the requirements of standards and regulations of the directives that apply to the product, including energy classification, gas used as refrigerant, climate class. One certificate to state that the refrigerator has been calibrated at the factory.Quality and safety standards met by the product must be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for the refrigerator, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the refrigerator.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the refrigerator within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each refrigerator to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Threeyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: FreezerCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The freezer is mainly used to store strains, enzymes and other temperature-sensitive reagents.Main specificationsEnergy classification A+.100% CFC- and HFC-free.One-door freezer, to be used as free-standing freezer.Capacity (gross): e"140litres.Dimensions (W D H): approximately 60cm 60cm 85cm.Net (interior compartment) W D H: approximately 47cm 43cm 69cm.Cooling system, static.Defrosting of freezing compartment, initiated manually.Temperature range of freezer compartment: 9C to 25C.Housing material and door: steel, coated, white.Door hinges right or left as desired, reversible.Fungus-resistant door gasket.Door with key lock.Adjustable feet for levelling.Interior container made of white plastic.Four shelves in the freezing compartment, at least three closed with a freezing flap.External digital temperature display for freezer compartment.Malfunction warning signal.Climate class SN-T.Refrigerant: Fluorocarbohydrogen free (R600a).Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 150 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. Declaration of conformity to the requirements of standards and regulations of the directives that apply to the product, including energy classification, gas used as refrigerant, climate class. One certificate to state that the freezer has been calibrated at the factory.Quality and safety standards met by the product must be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for the freezer, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the freezer.Installation and maintenance Any prerequisites for installation to be communicated to the purchaser in advance, in detail. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the freezer within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each freezer to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Three years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. B7 Detailed technical specifications for molecular biology techniques Name of equipment: PCR workstation Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The workstation is used to prepare reagents under clean conditions to avoid contamination with DNA.Main specificationsExterior dimensions (H W D): approximately 700mm 750mm 600mm.Interior working area (W D): approximately 700mm 500mm.Exterior: stainless steel or powder-coated metal.Interior: stable formed stainless steel.Side panels transparent, able to absorb wavelengths below 400 nm.Overhead UV light for DNA decontamination; two lamps, 25 W each.Separate, switchable, UV air-sterilizing circulation unit; UV lamp (25 W).Timer and key lock for UV lamp; timer operates only when key lock is on.Overhead white light; 15 W; at least 800 lux.At least two plug outlets built into the chamber; AC 230 10V; 50Hz; 5A fuse.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Depends on the electrical equipment used inside the workstation; maximum 1200 W. Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001 and a type-test certificate of relevant optical and mechanical tests.Quality and safety standards met by the product must be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the workstation. Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the workstation within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication materials, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each workstation to be accompanied by an authorized list of accessories and spare parts.Set of fuses for the workstation.Two UV lamps.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Twoyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: PCR thermocycler Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function The thermocycler is used in a TB laboratory to amplify fragments of mycobacterial DNA.Main specificationsThermocycler, Peltier elements.Block for 96 0.2ml tubes; possibility to use block with 48 0.5ml tubes and 96-well PCR plates.Blocks must be resistant to oxidation.Heating rate: 4 C/s. Cooling rate: 2 C/s.Temperature range (block): 4100 C.Regulating accuracy for block temperature: 0.1 C.Temperature uniformity at 70 C (block): 0.4 C.Internal memory for at least 50 programs with up to 99 steps/program, freely editable. Heatable lid with automatic height adaptation.Electromechanical lid blocking to prevent accidental opening during a run.Temperature range for lid: 80C to at least 103 C.Optional: Interface for remote control via PC; activated RS 232 serial port.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 500 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the thermocycler has been calibrated at the factory and certified according to ISO 13485 quality regulations.Quality and safety standards met by the product must be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the thermocycler.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the thermocycler within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication materials, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each thermocycler to be accompanied by an authorized list of accessories and spare parts.Set of fuses, if used separately in the instrument.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Twoyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity (as high as 90% at 35C), ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose products additional to the requirements listed above. Name of equipment: Microlitre centrifugeCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The centrifuge is designed for routine applications in molecular biology.Main specificationsRobust metal housing; compact design with chemical-resistant (coated) housing.Easy-to-clean, smooth rotor chamber that is resistant to acids, alkalines, disinfectants used in the laboratory.Low access height (d"23cm) and space-saving design (d"24cm 32cm; W D). Optional: Centrifuge with a built-in cooling system, adjustable in 1C steps; cooling down to at least 4C and able to keep this temperature at maximum speed. It should have a precool feature and stand-by cooling. Microprocessor-controlled centrifuge.Standard rotor with a capacity of at least 18 positions; aerosol-tight (chemical-resistant coated); exchangeable.Maintenance-free motor.Maximal relative centrifuge force: 15000G.Automatic lid lock, starting with and during run of rotor.Option: Automatic opening at the end of the run. Emergency unlock for electricity blackout.LCD display; protected; showing time and relative centrifugal force or speed in rcf or rpm.Speed adjustable in 100 rpm steps.If a keypad is used, it should be foil protected.Timer for runs between 30seconds and 30minutes and an option for continuous operation for longer runs.Short time operation by pressing a time button for short spin.Adjustment of running time in steps of 30seconds.Short acceleration time to maximum rcf in d"20 seconds.Short breaking time from maximum rcf in d"20 seconds.Noise level: d"58 dbA.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 250 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the centrifuge has been calibrated at the factory.Quality and safety standards met by the product must be listed.AccessoriesOptional: Adapter set; reduction device for smaller tubes to be centrifuged in the standard rotor to maximal rotor capacity, for 0.5ml and 0.2ml tubes.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the microlitre centrifuge.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the microlitre centrifuge within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders must specify the quantity of every item included in their offer (including items not specified above).Spare parts Each centrifuge to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: One year.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose additional products to the requirements listed above. Name of equipment: Ultrasonic bathCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The ultrasonic bath is used for disruption of bacterial cells to release DNA.Main specificationsRobust stainless steel housing.Bowl made of special cavitation-proof stainless steel.Working frequency: 35kHz or 37kHz.Timer for 130-minute use and continuous operating.Heater for water-bath; adjustable between 30C and 80 C.Safety shut-down after 12hours.Timer and heater adjustable by knob or foil keypad.Warning indicator for over temperature.Volume:1.52litres.Cock, outlet for water.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 100W for high frequency (HF) power and heating.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the ultrasonic bath has been calibrated at the factory.Quality and safety standards met by the product must be listed.AccessoriesSwimming racks; foamed polyvinyl chloride (PVC) or PE; heat resistant to e"80 C; positions for 1.5 or 2.0ml standard reaction tubes.Optional: Swimming racks; foamed PVC or PE; heat resistant to e"80C; positions for 0.5ml standard reaction tubes.Optional: Swimming racks for larger test-tubes.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the ultrasonic bath.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the ultrasonic bath within 14days.Standard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).Spare parts Each ultrasonic bath to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Two years.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose additional products to the requirements listed above. Name of equipment: Thermo-shaker Code number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use The thermo-shaker is used especially for molecular diagnostic test systems such as hybridization and incubation within a defined temperature profile.Main specificationsSturdy, compact housing; coated to resist corrosion; resistant to chemicals, alcoholic disinfectants and 1.5% hypochlorite. Standing on four rubber feet designed to prevent sliding of the instrument during shaking.At least two Peltier elements.Thermo-blocks resistant to 1.5% hypochlorite solution.Optional: Exchangeable thermo-blocks, at least one with trays for 12hybridisation strips and one with 24 slots for the incubation of 1.5ml standard reaction tubes.Optional: Removable transparent lid.Incubation temperature: ranging from 4C to 99 C in steps of 1 C.Heating rate: 5 C/second. Cooling rate: 4 C/minute from 99C to 20C, and 1C/minute from 20C to 4C.Accuracy of incubation temperature: at least 0.5 C.Shaking time: adjustable from 0hours to at least 72hours.Shaking frequency for hybridisation block: 0 (no shaking at all) or 150400 rpm in steps of 50 rpm.Mixing amplitude: approximately 3mm.Memory for at least six programs, with different program steps for temperature and shaking; at least 10 program steps.Display for 48 good readable digits; LCD; hermetically sealed against droplets (liquid splashing).Keyboard for programming, also sealed against droplets.Operating temperature and humidity range: 035 C, 70% relative humidity.Electricity requirementsSupply voltage: 230 10V, AC, 50/60Hz. Voltage and plugs to be adapted to meet the country requirements.Power consumption: Approximately 150 W.Conform to electrical safety standards IEC 606011, UL 610101, EN 610101.Protection class (in accordance with EN60529).Designed not to interfere with circuit radio (in accordance with EN55014).DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the thermo-shaker has been calibrated at the factory.Quality and safety standards met by the product to be listed.AccessoriesThermometer suited for regular temperature checks; to feature a wire plunge sensor (e.g. Pt100) and a metering precision of 1/10 class B (AT=0.03C) in the range of 0100C.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the thermo-shaker.Installation and maintenance The bidder must arrange for the equipment to be installed by certified or qualified personnel; any prerequisites for installation to be communicated to the purchaser in advance, in detail. The bidder to also provide user training (including how to use and maintain the equipment) and a comprehensive maintenance plan. The cost of the maintenance plan to be defined and guaranteed over the period of warranty. The supplier to provide an after-sale service that covers the whole country. The service to have competent staff, adequate infrastructure and sufficient spare parts to be able to respond to any complaints and to repair or replace the thermo-shaker within 14days.Spare parts Each thermo-shaker to be accompanied by an authorized list of accessories and spare parts.One extra lid.Set of fuses.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: At least twoyears.Remarks The equipment offered, including its power supply, to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C, fungi, and spikes in the electricity supply. Bidders may propose additional products to the requirements listed above. Name of equipment: Microlitre pipettesCode number: Tender specificationsBidders specificationsQuantity: Manufacturer: Type/model:Country of origin:Description of function and use Microlitre pipettes are used for molecular biology procedures; a set of different volumes is required.Main specificationsSingle-channel microlitre pipettes.Fully autoclavable (121C); UV-resistant material.Adjustable volume range of each pipette: a. 0.510 l b. 10100 l c. 1001000 l.Increments: a. At least 0.1 l fine adjustment b, c. At least 0.5 l fine adjustment (0.1 l preferred).Accuracy: a. At least51% (first value applies to smallest volume, last one to the largest volume in the stated range). b, c. At least 30.6% (first value applies to smallest volume, last one to the largest volume in the stated range).Precision: a. At least30.4% (first value applies to smallest volume, last one to the largest volume in the stated range). b. At least0.70.2% (first value applies to smallest volume, last one to the largest volume in the stated range). c. At least0.30.2% (first value applies to smallest volume, last one to the largest volume in the stated range).Three defined stops (single-button operation preferred): take-up from the first stop dispensing and blow out tip ejection.Easy and safe tip ejection mechanism.Fixation of adjusted volume.Slim pipette shaft. Cone for standard tips.DocumentationManufacturers certificate The manufacturer must have a management system certified to ISO9001. One certificate to state that the pipette has been calibrated at the factory.Quality and safety standards met by the product must be listed.Operation, maintenance and installationOperation and maintenance manual At least one set of operation, maintenance and service manuals for each microlitre pipette, written in UN languages (or at least in English) and preferably also in the official national language of the country requesting the microlitre pipette.Installation and maintenance The supplier to provide an after-sale service that covers the whole country. 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|kdNY$$IfF0;d%) t0'644 laHISTcdooooh$Ifgd l |kdY$$IfFx0;d%) t0'644 ladefrslYYYh$Ifgd l kdZ$$IfF4wF;8d%, t0'6    44 laf4stulYYYh$Ifgd l kdL[$$IfF4WF;8d% , t0'6    44 laf4lYYYS$Ifgd l kd\$$IfF4wF;8d% , t0'6    44 laf4&)/0@BLfijknoABNRZ\_a23@DLMPQRT h~Gh{h h h h{h h~Gh h|55hch 5 h 5P'(q^S$Ifgd l h$Ifgd l zkd\$$IfF0;d%) t0'644 la()MNqqQ$Ifgd l zkd[]$$IfF0;d%) t0'644 laNOqqQ$Ifgd l zkd]$$IfF0;d%) t0'644 laqqqqqQ$Ifgd l Tzkd^$$IfF0;d%) t0'644 la CDqqqqQ$Ifgd l \zkd _$$IfF0;d%) t0'644 laDEO45qqqqQ$Ifgd l zkd_$$IfF0;d%) t0'644 la56A%qqqqqQ$Ifgd l zkdN`$$IfF0;d%) t0'644 la#$(013678:{ =>\]rs"+OPRn h,h h 5h,h 5 hh h~Ghh h~Gh{ h h h~Gh h h{J q^^^qV$Ifgd{l Q$Ifgd l Tzkd`$$IfF0;d%) t0'644 la?@qqQ$Ifgd l \zkd|a$$IfF0;d%) t0'644 la@A^_qqQ$Ifgd l zkdb$$IfF0;d%) t0'644 la_`uvqqQ$Ifgd l zkdb$$IfF0;d%) t0'644 lavwqqQ$Ifgd l TzkdAc$$IfF0;d%) t0'644 laq^Q$Ifgd l h$Ifgd l zkdc$$IfF0;d%) t0'644 laPQqqqqQ$Ifgd l zkdod$$IfF0;d%) t0'644 laQRqqQ$Ifgd l Tzkde$$IfF0;d%) t0'644 laq^Q$Ifgd l h$Ifgd l zkde$$IfF0;d%) t0'644 laq^^Q$Ifgd l Q$Ifgd l zkd4f$$IfF0;d%) t0'644 la  BFVz ";<=@CNOQRSX|h 5h hK7h U h h h^h h~Gh h~Gh 5h h,h 5 h 5Lq^KQ$Ifgd l Q$Ifgd l Q$Ifgd l Tzkdf$$IfF0;d%) t0'644 laStandard maintenance tools All standard accessories, consumables and parts required to operate the equipment, including all standard tools and cleaning and lubrication material, to be included in the offer. Bidders to specify the quantity of every item included in their offer (including items not specified above).A maintenance kit, with full documentation and tools for in-laboratory calibration according to ISO9000, are part of the procurement.Spare partsGaskets.Lubricants.Each microliter pipette to be accompanied by an authorized list of accessories and spare parts.Packing data Packing data are not necessarily part of the bidding process, but are needed for shipment and for customs declarations.Net weight.Gross weight.Dimensions (W H D) in cm.Appliances must be transported upright (Y/N).Customers tariff number.Warranty: Two years.Remarks The equipment offered to be designed and constructed to operate properly and continuously in the conditions of the purchasers country; the equipment may need to tolerate high humidity, ambient temperatures of 540C and fungi. Bidders may propose additional products to the requirements listed above. Name of equipment: Emergency spill kit, cleaning setCode number: Tender specificationsBidders specifications Quantity: Manufacturer: Type/model:Country of origin:Description of function The kit is used in case of accidental spillage of infectious material in the laboratory.Main specificationsFFP3 mask with expiration valve; three pieces.Head cover; two pieces.Protection glasses; two pieces.Overall, single use; two pieces.At least 10 pairs of overshoes.At least five autoclave-proof plastic bags; 1000mm 700mm; with biohazard sign.At least two pairs of thick-walled household rubber gloves (to pick up shards and sharps).Disposable gloves; one package of each size (small, medium and large).Forceps or crucible tongs to pick up sharps.Container for sharps.Absorbent paper tissue; at least 500mm 500mm; 1kg.MTB-approved concentrated disinfectant for dilution.Bottle with spray head, 1litre.Alcoholic disinfectant, 1litre.Concentrated formalin solution (>30%), 1litre; fumigation device.5litre pressure spray bottle for disinfectant. Annex B1 Conditions of electricity power supply This annex provides definitions and explanations to make countries aware of potential problems with electricity power supply that could affect operator safety and the life expectancy of equipment. Spikes In developing countries, power fluctuations in the electricity grid are common, and alternating current (AC) power lines are subject to many different kinds of voltage disturbances. Spikes that is, pulses of high voltage and current are generally caused by equipment with high energy requirements (e.g.an elevator) being switched on or off, or by lightning. Spikes usually last for only microseconds. Transistors used in almost all electronic devices (e.g.motherboards and regulatory devices) are sensitive to spikes and need be protected. Power boards (also known as power strips or multi-outlet power boards) with protection against power spikes are available. If lightning is frequent, boards with protection against spikes of more than 10000volts (V) and 10000amperes (A) should be purchased. For areas without lightning, protection against spikes of more than 3500V and 3500A should be sufficient. Power boards with protection against spikes are not expensive, but they can be unreliable or of poor quality. To ensure that high-quality boards are purchased, it is best to check reports on the internet on the quality of different types of board. Surges and sags Surges and sags are periods of high or low voltage; they are less severe than spikes but last from a few seconds to a few minutes. Surges and sags are usually caused by faults with the power suppliers or by other devices on the line. Electrical laboratory equipment is usually designed to tolerate 15% fluctuation in the operating voltage. If local fluctuations exceed this range, a voltage stabilizer should be installed. The cost of a voltage stabilizer will depend on the power consumption of the connected devices. Heating coils are not usually susceptible to fluctuations (lower voltage simply prolongs the duration of heating); therefore, it is advisable to check whether the regulatory part of the electrical set-up of individual items of equipment (e.g. incubators, hot-air ovens and distillers) can be stabilized separately. Brownouts and blackouts Other problems with electricity supply include: brownouts longer sags lasting several minutes or hours; and blackouts periods of (near) zero voltage, usually caused by suppliers or by blown fuses or tripped breakers. Depending on the duration of the blackouts, an electric generator may be needed. To safeguard against brownouts or blackouts lasting for several minutes, a UPS is needed. The battery life needed in the UPS will depend on the average duration of blackout and the time needed to safely finalize the work that uses the laboratory equipment (e.g.about 20minutes for BSCs and centrifuges). Return surges (i.e.potentially damaging periods of high voltage) often occur when power returns at the end of a blackout; they can also result from laboratory generators being started. Grounding It is important to check that the electricity supply to the laboratory is properly grounded at the point where the supply enters the building and after distribution of phases at each plug. The frequency required for new equipment should be checked, because an electrically driven motor will lose most of its power if connected to the wrong frequency. For more details and specific advice for a country, or to solve specific problems, it is advisable to contact an electrical engineer. Annex B2 Biological safety cabinets This annex provides important information for those ordering BSCs. BSCs with a directed airflow can provide protection for laboratory staff and reduce the risk of airborne infection. Unfortunately, the terminology for this equipment is often ambiguous, which sometimes causes laboratories to procure the wrong equipment. This annex sets out the difference between laminar flow hoods and BSCs, to ensure that laboratories are aware of the important differences in the design and intended use of these two types of equipment. Laminar flow hoods Laminar flow hoods are also known as laminar airflow units, horizontal and vertical outflow cabinets and clean-air workstations. They must NOT be used for handling infectious agents. These hoods are simply designed to create clean and dust-free conditions in laboratory working areas, and are used to prevent contamination of sensitive devices in industries such as pharmaceutical manufacturing and biotechnology. In a laminar flow hood, air is drawn from a high-efficiency particulate air (HEPA) filter, and blown in a smooth, laminar flow over a working area into the room. Cabinets are usually constructed from stainless steel, with no joins or gaps, to prevent attachment of spores and growth of microorganisms. Types of laminar flow hoods As shown in FigureAB2.1, airflow in laminar flow hoods can be horizontal or vertical. Whatever the type of airflow, laminar flow hoods provide product protection only. Laminar flow hoods must NOT be used when working with any form of biological or chemical hazard, because the operator and the surrounding environment will be exposed to any potentially infectious aerosol created on the workbench. Horizontal airflow benches can be used for preparing culture media or handling other noninfectious reagents or solutions, but must NOT be used with potentially carcinogenic or allergenic materials. Vertical airflow benches also blow air out into the room.  HEPA: high-efficiency particulate air Red arrow: room air Green arrow sterile air FigureAB2.1 Laminar flow hoods Biological safety cabinets BSCs protect people and the environment from infectious agents and depending on the classification may offer varying degrees of particle protection for products. Infectious agents must be handled in a BSC, NOT in a laminar flow hood. Classification of biological safety cabinets Class I In a classI BSC (shown in FigureAB2.2), room air is drawn in through a front opening at a constant velocity. The air passes over the work surface and is discarded from the cabinet through the exhaust duct. Aerosols or other particles that might be generated during work are sucked away from the laboratory worker by the directional airflow into the duct. The front opening allows the operator to perform the work while observing through a window screen. According to EN 12469, a carefully maintained inward flow of room air of between 0.7m/s and 1.0m/s should be established. Although airflow is directed away from the worker, the air is not HEPA filtered, so products are not protected. Air can be hard ducted out of the laboratory or extracted through a thimble if external exhaust fans are installed. If possible, the external exhaust fan should be located at the very end of the exhaust pipes and interconnected with the BSC fan, so that the BSC cannot be switched on unless the external extractor fan is on. Maintaining a slightly negative pressure in the duct will prevent leakage of contaminated air into the laboratory.  HEPA, high-efficiency particulate air Figure AB2.2 Schema for airflow for a classI biological safety cabinet Class II A classII BSC is designed to extend the protection features of classI, to protect sensitive materials from contaminated room air. Types of classII BSC include: class II (EN 12469), which complies with European norm standards; and classIIA1, classIIA2, classIIB1 and classIIB2 (NSF 49), which comply with National Sanitation Foundation standards (United States). A classII BSC differ from a classI BSC in that design features in the classII allow only HEPA-filtered air to flow over the work surface. This air is sucked through the front and back grid, back to the system. As shown in FigureAB2.3, classII BSCs have a front access opening and a carefully maintained inward flow of room air though a front intake grill. The air supply passes through a HEPA filter before flowing downward as a vertical (ideally laminar) flow. The airflow is split just above the working surface so that about half enters the intake grill (together with newly entering room air) and the other half passes through the rear exhaust grill. Any particles generated at the working surface are captured by the downward flow and eliminated. The total air is then discarded through the rear plenum into a space between the supply and the exhaust HEPA filters. The relative size of both filters determines the percentage of recirculated air passing through the supply filter. If possible, the exhaust air from a classIIA BSC should be ducted out through a thimble connection to a dedicated duct with an extraction fan outside. The exhaust air from a classIIB BSC should be hard ducted to the outside. A separate extractor fan with sufficient capacity is needed when the BSC is part of the ventilation system, to create and maintain a lowered pressure in the laboratory. Laboratories should: discourage use of classIIA1 BSCs because they may have contaminated ducts and plenums at a positive pressure to the room; and ensure that classIIB1 and classIIB2 BSCs are hard ducted to the outside; this means that the building exhaust system must be precisely matched to the airflow requirements specified by the manufacturer for both volume and static pressure; thus, certification of these BSCs is more difficult.  Figure AB2.3 Class II A2 Biological Safety cabinet, air flow pattern As shown in TableAB2.1, NSF49 differs from EN12469. TableAB2.1 Comparison of requirements for NSF49 and EN12469 NSF 49EN 12469AirflowClass INot specified.Inward flow of e"0.7m/s and d"1.0m/s.Class IIClassIIA (with an inward flow of e"0.38m/s) should be discouraged because they may have contaminated ducts and plenums at positive pressure to the room. ClassIIA2 have an inward flow of 0.5m/s.Does not differentiate between classIIA1 and classIIA2; the inward flow should be at least 0.4m/s, and above this value according to manufacturers specifications.DownflowAll typesRequires compliance with the manufacturers set points or downflow velocity within a deviation of 0.025m/s from a nominal set point.Requires airflow velocity to be >0.25m/s and <0.50m/s, and defined by the manufacturer according to the equipment design. No individual measurement should differ by more than 20% of the value requested by the manufacturer. Class III ClassIII BSCs are hermetically sealed, with two HEPA filters on the exhaust system, and all procedures conducted through arm-length rubber gloves. Class III BSCs are used in high-level (Level 4) containment labs and are NOT required in a TB laboratory. Certification of BSCs A BSC must be checked and certified for biosafety and all other functions before delivery. The test results must be documented and provided with the BSC. The packing must have an indicator stating that the cabinet was kept in an upright position during the entire transportation. The following tests of a BSC are required in the laboratory: airflow smoke pattern tests; air inflow velocity test; air downflow velocity profile test; HEPA filter leak test; alarm function verification; exhaust system performance; cabinet integrity test (for classIIA1 cabinets with positive-pressure contaminated plenums only); and ultraviolet germicidal intensity (UVGI), if installed. The following tests of a BSC are also recommended: lighting intensity test; vibration test; noise-level test; and electrical tests (leakage, ground circuit resistance and polarity). Frequency of testing A BSC should be tested: at initial installation (on site, before initial use); at least annually after initial installation; after replacing filters; and after repair work is carried out or the cabinet is moved. Prerequisites for laboratory preparedness before installing a BSC This section provides information that will help laboratories to avoid major pitfalls in planning and designing TB laboratories. Issues to consider in locating a BSC are as follows: In general, a BSC must be located away from main traffic areas, doors and air-supply or exhaust diffusers that may disturb or interrupt airflow. A BSC plus its stand is usually 2.152.30m high. The exhaust air must be ducted out (and, in some BSCs, exhaust filters have to be changed from the top); therefore, space should be left between the exhaust grid and the ceiling of the laboratory. An exhaust pipe with a cross-section of about 400cm (20cm 20cm) is commonly used to prevent resistance to the airflow; hence, the room ceiling should be high enough to accommodate the BSC and exhaust pipe. Whenever possible, a clearance of 30cm or greater should be provided on each side (including back to wall) of the cabinet to allow for access. If recirculation is used (not recommended), a minimum clearance of 30cm must be provided between the exhaust outlet on top of the cabinet and any overhead obstructions. For installation of an exhaust system with appropriate cross-section and thimble construction (see AnnexB3), a minimum clearance of 45cm will be required. Issues to consider in ensuring that a BSC is used safely are as follows: For ducted cabinets, the extractor fan on the exhaust system should be located at the terminal end of the ductwork. Failure of the exhaust airflow should signal an alarm to the user. To prevent pressurization or failure of exhaust flow from the cabinet, when hard ducted out or with bypass construction, an anti-backflow device to prevent reverse airflow through the HEPA filter may be required. Options for connecting a BSC are as follows: direct connection cabinet and the external ventilator have to be switched on at the same time; direct connection with a bypass an anti-blowback valve is needed; the external ventilator may work for 24hours a day; when the cabinet is switched off, the bypass valve in the duct will open in order to extract the air from the room; and connection via a thimble construction (an option not valid for class IIB BSC) this option is illustrated in Annex B3. Proper planning for heating, ventilation and air-conditioning will be the work of an engineer. The staff available locally for maintenance should also be taken into account. Laboratories should plan properly before requesting tenders for a BSC. The following professional associations may be useful in the planning process: American Biological Safety Association http://www.absa.org/index.html American Industrial Hygiene Association, Biosafety  HYPERLINK "http://www2.umdnj.edu/eohssweb/aiha/technical/biosafety.htm" http://www2.umdnj.edu/eohssweb/aiha/technical/biosafety.htm American Society of Microbiologists http://www.asm.org/policy/ Annex B3 Thimble connection This annex discusses the construction of a thimble connection within a BSC. A thimble connection (shown in FigureAB3.1) requires the installation of an external extractor fan, preferably located at the very end of the exhaust pipes. A slightly negative pressure maintained in the duct prevents leakage of contaminated air into the laboratory. A thimble exhaust system is NOT suitable for a classIIB BSC.  BSC, biological safety cabinet; HEPA, high-efficiency particulate air FigureAB3.1 Schematic representation of thimble connection The external fan may be switched on separately from the BSC. Alternatively, the fan can be coupled via a relay circuit with a caster so that when the BSC is switched off, the external fan still runs for some time. The BSC should be equipped with an interconnection, so that the BSC cannot be switched on unless the external extractor fan is also turned on. According to national regulations for some countries, an additional HEPA filter, preferably located at the beginning of the exhaust pipe right after the influx of room air, is required to protect the external environment from potentially contaminated air from the laboratory room. The external fan must be efficient enough to ensure negative pressure in the duct despite the additional HEPA filter and its expected loading. When the external fan is switched on, air will be extracted from the room according to the setting of the extractor fan, which should be regulated according to the air volume rate needed to establish the desired lowered pressure within the laboratory room. If the BSC is also switched on, its exhaust air and some of the additional room air will be expelled (as long as the total exhaust air rate of the BSC is less than the adjusted volumetric flow rate of the extractor fan). Advantages of a thimble connection Advantages of a thimble connection are that: no adjustments have to be made to the cabinet, and the pressure in the room will be nearly constant; to keep a controlled constant lower pressure (e.g. 30Pa) within the containment room, a damper for the exhaust or supply air (or both) will be needed; the components include controller electronics and airflow measurements; and in case of a blackout, the air flowing back to the room with lowered pressure will pass through the thimble and will not wash off bacteria from the HEPA filter. Anti-blowback valves and fire flaps are usually recommended, depending on the climatic conditions. A heat exchanger, humidifier or air-conditioning may also be advisable. If more than one BSC is installed, laboratories should take precautions to balance the exhaust air from the room, depending on which BSC is switched on (compensation for the airflow resistance in the exhaust pipe) and how many cabinets are switched on at the same time. Some manufacturers offer thimbles with motorized flaps that can be regulated for this purpose. To balance needs and costs at a particular setting and obtain a suitable solution, laboratories should consult an engineer qualified in heating, ventilation and air-conditioning. 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