ࡱ> *,'() Hbjbj 7<^^XXXlll88lx"(DDD`'`'`'kxmxmxmxmxmxmx${}xX''">'"''xDDx///'<8D8Dkx/'kx//?of@%vDsj)FrFWxx0xr:Z~c)XZ~%v%v&Z~XKv `'t'/' 'S`'`'`'xx,`'`'`'x''''Z~`'`'`'`'`'`'`'`'`'^ ~: About This Document The section supervisor and appropriate staff members review the procedures contained in this document annually. Any changes are also reviewed by the Quality Assurance Officer and the designated ASCLD-LAB Director. All Changes are signed. Old procedures are archived and retained in the laboratory for at least two years. TABLE OF CONTENTS INTRODUCTION Principle Specimen Requirements Chemicals Safety Apparatus Preparation of Solutions QC and Sample Run Scheme Evidence Handling and Preservation Specimen Handling Quality Control Equipment Maintenance and Calibration Reagents, Standards, and Quality Control Materials Quality Control FREQUENCY of Updating calibration curve evaluation and reporting results outside the range of calibration REPORTING UNCERTAINTY OF MEASUREMeNTS Sample Preparation Gas Chromatographic Analysis Calculation and Reporting of Results Notes Case Documentation Case Notes Case File References INTRODUCTION Forensic alcohol analysis is defined as the practical application of specialized devices, instruments and methods by trained laboratory personnel to measure the concentration of ethyl alcohol in samples of blood from persons involved in traffic accidents or traffic violations. This activity is carried out in laboratories certified by the Maine Department of Health and Human Services (D.H.H.S.). In order to be so licensed, personnel must meet the requirement set forth by DHHS rule 10-44 Chapter 267 - CERTIFICATION STANDARDS FOR PERSONS CONDUCTING CHEMICAL ANALYSES OF BLOOD AND BREATH FOR THE PURPOSE OF DETERMINING THE BLOOD ALCOHOL LEVEL. The Maine Health & Environmental Testing Laboratory meets the above criteria and is a certified forensic alcohol laboratory. The method selected for the determination of alcohol content of blood samples utilizes a headspace gas chromatograph to perform a test that is both qualitative and quantitative. The procedure calls for addition of a small aliquot of sample to an internal standard solution. A portion from the headspace of this mixture is injected onto gas chromatographic columns that are capable of separating ethyl alcohol from acetone and the common aliphatic alcohols (i.e., methanol, isopropanol, etc.). Quantitation is accomplished through comparison to calibration curves. Data is captured and calculations are performed by device(s) designed to do so (i.e., integrator, workstation, laboratory automation computer). A copy of the method description is immediately available at all times in the work area and is available for inspection at the Department of Health and Human Services upon request. PRINCIPLE Aliquots of biological fluids or liquids are mixed with an internal standard solution. The samples are then analyzed by headspace gas chromatography and quantitated using the internal standard technique. SPECIMEN REQUIREMENTS 1. Whole blood 2. Serum, plasma, or urine 3. Other biological fluids 4. Liquids or beverages Chemicals Methanol Ethanol Isopropanol n-Propanol Acetone Acetaldehyde Deionized water Safety Precautions and PPE Lab coats, gloves and eye protection will be worn when handling chemicals. Full-face shield will be worn when handling blood samples. APPARATUS Model 7694 Agilent Headspace Sampler Agilent 7890A Hewlett-Packard gas chromatograph Detector Dual Flame Ionization (FID) Columns Restek RTx BAC 1; 30 meter; 0.53mm ID; 3.0 um df Restek RTx BAC 2; 30 meter; 0.53mm ID; 2.0 um df Carrier Gas Helium UHP Detector Gas Hydrogen Zero Headspace Parameters Shake vial 1min. (slow) Stabilize time 1 min. Vial pressure 10.6 psi Carrier pressure 12.4 psi Oven temp. 50oC Loop temp. 80oC Transfer line temp. 80oC Cycle time 4.1 min. Vial equil. 10 min. Pressurization time 0.13 min. Loop fill time 0.15 min. Loop equil. Time 0.15 min. Inject time 0.20 min. Chain methods 1+1 Check GC Ready No GC Parameters - Oven temp. 40oC 7890A Inlet temp. 180oC Inlet Pressure 11 psi Detector temp. A&B 240oC Inlet B 180oC Init. Temp 40oC Init. Time 3.00 min. Oven Equil. Time 0.50 min. Oven Max. 225oC Ambient 25oC Oven ON Init. Value A&B OFF On Time A 0.75 min. On Time B 0.00 min. Off Time A 0.00 min. Off Time B 0.00 min. Save Data BOTH Detector A&B ON Peak Width 0.053 Data Rate 5.000 Data Storage ALL Attn. Sig. 1 3 Attn. Sig. 2 4 Offset Sig. 1 10 Offset Sig. 2 10 Time Sig. 1 5.0 Time Sig. 2 5.0 Quant Report Summary/Printer Preparation of Solutions Internal Standard: 0.10% by weight n-propanol Dilute 1.0 mL n-propanol to 1.0L with deionized water Ethanol Standards: Purchased from a suitable vendor (such as Lipomed) Whole Blood Controls from a suitable outside vendor (such as UTAK Laboratories). Volatile Mixture Solution: In a 100mL volumetric flask add 100uL each of methanol, ethanol, isopropanol, 50uL of acetone and 20uL of acetaldehyde. Make up to 100mL with deionized water. Calibration Solutions: Purchased from a suitable outside vendor (such as Cerilliant) 0.010g/dL, 0.050g/dL, 0.100g/dL, 0.150g/dL, 0.200g/dL, 0.300g/dL and 0.400g/dL and a multicomponent alcohol mix. QC and Sample Run Scheme: Every sample is run in duplicate. The following is an example of a run: Water Blank ETOH Standard 3 samples in duplicate ETOH Standard 3 samples in duplicate ETOH Standard 3 samples in duplicate ETOH Standard 3 samples in duplicate ETOH Standard 3 samples in duplicate ETOH Standard 2 samples in duplicate ETOH Standard Whole Blood Controls (QA Range as stated by manufacturer) Volatiles Test Mixture Chromatography Resolution Refer to Appendix A for sample run sheet. Evidence Handling and Preservation SPECIMEN HANDLING All laboratory personnel will handle submitted materials in a manner that assures the integrity of the evidence. Prior to initiating and during the processing of evidence, the analyst will employ the following practices: The work area will be clean and free of any debris Countertops are cleaned after each case/item or when dirty All glassware and tools to be used will be clean Test tubes, capillary pipettes and Pasteur pipettes are used only once, then discarded To prevent cross contamination of samples, only one case will be opened by the analyst at a time Reagents and solvents will be kept in closed containers During analysis the evidence will be under constant control by the analyst. Evidence to be analyzed will be removed from evidence refrigerator and the reverse side of the pink Receipt/Request for Examination Form will be filled out. The analyst will initial all the stickers bearing the Lab Identification Number. If the subjects name is not available at the time of log-in, the analyst will write the subjects name on the label at the time of analysis. The analyst will ensure all identification numbers and names agree with the Chain of Custody receipt. The collection kit and all specimens will be labeled with the lab identification number, name of the subject, and the analysts initials. All paperwork contained in the kit will be labeled with the laboratory identification number. The analyst will verify and note in the case notes that the case information provided with the kit matches the HETL folder, sample information from the Blood Alcohol Analysis form submitted with the sample and all Starlims labels. The analyst will document the HETL case number on the worksheet. The analyst will record the lot numbers of the standards, control and calibrators on the worksheet At the time of analysis a worksheet with the specimen identification number will be created. After analysis the remaining blood tubes will be sealed in an evidence bag and the seal initialed and the bag labeled with the laboratory identification number by the analyst and placed in the appropriately labeled sample bin located in the evidence refrigerator. The sample kit will be stored in an appropriately labeled box. This box will be retained until filled. All filled boxes will be placed in storage until returned to the submitter or destroyed. Quality Assurance A. Equipment Maintenance and Calibration Refer to Quality Assurance Manual B. Reagents, Standards, and Quality Control Materials Refer to Quality Assurance Manual C. QUALITY CONTROL Function checks will be performed to check the performance of equipment and agents used (either at regular intervals or while testing samples). Control checks will be performed during the analysis or testing process. These checks are used to: Determine the performance of the analytical or testing system. Quantitate the variability of results from the analysis or test in terms of precision and accuracy. The frequency of checks will be determined by: Currently accepted practices/standards in the discipline. The number of samples being run in a particular sequence. Wherever possible, Control Charts will be set up and used to record results from selected function and control checks. Determination will be made whether the testing or analytical process is out of control and corrective actions taken will be recorded. Control checks will be performed during the analytical or testing process. These checks are performed either with each analysis or intermittently after a specified number of analyses. These control checks include but are not limited to: Blanks Standard with known or established specifications. Running samples in duplicates or triplicates. Controls Calibrators and standards shall be from different sources. Ethanol Calibration Standards NIST traceable standards will be used for calibration Ethanol Daily Standards NIST traceable standards will be used for standards FREQUENCY OF UPDATING THE CALIBRATION CURVE A calibration will be run once per week when samples are analyzed. This data will be stored with the ethanol control documents Criteria for acceptance will be: 1) A minimum of 4 points; and 2) An r2 value of .998 or greater E. Evaluation and reporting results outside the range of the calibration curve Any sample determined to be outside the range of the calibration curve (> 0.40) will be: 1. diluted and re-run or 2. reported as over the upper calibration limit REPORTING UNCERTAINTY OF MEASUREMeNT When estimating the uncertainty of measurement, all uncertainty components which are of importance shall be taken into account using appropriate testing procedures. The documentation containing the pertinent information (QAM 5.4.6.3), is located in Appendix B SAMPLE PREPARATION A. Liquid samples: 1. Mix the sample thoroughly 2. Label two 20mL headspace vials with identification number and suffix (A or B). 3. Using the auto dispenser re-pipetter - pipette two milliliters of the internal standard solution into each vial. 4. Pipette an appropriate aliquot of sample (typically 1 mL with a 250 L minimum) into each vial. If less than 1 mL Q.S. with diH2O to 1 mL 5. Seal the vial by crimping the vial cap. 6. Vortex the vial. GAS CHROMATOGRAPHIC ANALYSIS Check helium and hydrogen tanks and replace if necessary. Load vials on headspace autosampler. Verify the correct order of samples placed in the sample tray; Set vial parameters on headspace control panel (first vial, last vial). Store method 1. Load method 1. Check carrier pressure should be ~12.4 psi. Turn on hydrogen at the main valve on the tank. On QC console press Front Detector. Use the arrow buttons to move the cursor down to Flame. Press the on/yes button to light the detector. Repeat the process for the back detector. Check detector signals on GC and re-light if necessary. Load ETHANOL method on chemstation on computer. Create a run sequence by entering the sample numbers in the sample log table. Save the sequence in the form of C:\MSDCHEM\3\SEQUENCE\mmddyy. Save the data path in the form of C:\MSDCHEM\3\DATA\mmddyy. Simulate the sequence and say yes to view it and print it. Press start button on headspace control panel Run the sequence. At the conclusion of the run shut off the detector flames and shut the valve on the hydrogen tank. NOTE: Make notation on the worksheet of any instrument repair and/or removal of any vials (specify sample numbers) which occur during the analytical run CALCULATION OF RESULTS  1. Record all test results to three decimal places on work sheet. 2. For each sample, average the results and round down to three decimal places. 3. Blood results are reported in grams of alcohol per 100 mL of blood. 4. Serum/Plasma results will be converted to whole blood with the conversion factor 1.22:1* 5. Concentration of ethanol below 0.010 g/100ml will be reported as Below minimum reporting limit 6. Once the results are entered into the computer, the final reports will be generated and subject to review and signature. CALCULATION OF RESULTS (continued) 7. Have final reports, worksheets and chromatograms of samples and standards reviewed by another chemist. (Technical Review) 8. When reports and worksheet are successfully reviewed, make copies of the worksheet for each sample file folder. 9. Place worksheets and chromatography printouts for each sample into the corresponding sample file folder and a copy for the electronic quality control records. The analyst will initial each page. 10. Place original worksheet, sequence list and chromatography printouts for calibrators and standards into the Ethanol Controls folder labeled with the run date. File this folder in an archives box. 11. Have administrative review done on the file folders. * Measuring Blood Alcohol Concentration for Clinical and Forensic Purposes, AW Jones and Derrick Pounder, Handbook of Drug Abuse, S Karch, MD, 1998 VII. NOTES Blood alcohol concentrations > 0.08% are prima facie evidence in operating under the influence violations. Many volatile substances can be detected by this procedure. The most common volatiles in body fluids are ethanol, methanol, isopropanol, and acetone. All of these substances can be separated from ethanol on the gas chromatograph. VIII. CASE DOCUMENTATION A. CASE NOTES The minimum information, which must be contained in the case notes are: Laboratory Identification Number Collection kits suspect/police information paperwork Run Data QC Data Comments/Results All case notes, spectra and other data generated during analysis will bear the initials of the analysts and case number. All spectra and data generated and maintained in the ethanol control batch file will bear the initials of the analysts. An example of the laboratory worksheet is contained in the Quality Assurance Manual B. CASE FILE The minimal information, which must be contained in the individual case file consists of: The final report Any preliminary, supplementary or corrected reports Collection kits suspect/police information paperwork Worksheet Evidence receipt Original chromatograms Technical and Administrative Review REFERENCE Parker, K.D. , et.al., Gas Chromatographic Determination of Ethyl Alcohol in Blood for Medicolegal Purposes, Analytical Chemistry, 34 1234, 1962      Doc.#- Blood Alcohol Analysis Procedures - M002 DATE 02-20-2015 Approved by: Lab Director Page  PAGE 1 of  NUMPAGES 13 Printed Copy is the Controlled Copy The acceptable limits of accuracy for the standards, sample replicates during the run are as follows: + 0.005 g/dL or + 5%, whichever is greater. If the final result exceeds the top calibrator (.400), the sample will be diluted and re-analyzed. If the standards do not agree within the acceptable limits of accuracy, a new standard will be prepared. All samples which bracket the standard will be re-analyzed. If sample replicates do not agree within the acceptable limits of accuracy, new aliquot(s) will be prepared. The four lowest results which meet the accuracy requirements will be averaged and reported. If the problem persists, an investigation will be conducted to identify and correct the source of the error. Findings and corrective action(s) will be recorded. 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