ࡱ> wyv  bjbjT~T~ 0,66&&iiiii}}}}}1tp&&&t1v1v1v1v1v1v1$i3 6:1i&&&&&1ii 1^^^&iit1^&t1^^.1!r#q}<"x/`1101/E7^E741E7i1L&&^&&&&&11^&&&1&&&&E7&&&&&&&&&& /: Animal Care and Use Webquest Introduction Virtually every major medical advance of the last century has depended upon research with animals. Data from experiments on humans are obviously the most scientifically reliable; however, in many cases human research is ethically unacceptable. Researchers must first use animals, the living systems most closely related to humans, before humans are asked to participate in experimentation. Animals serve as surrogates in the investigation of human diseases and new ways to treat, cure or prevent them. The scientific community recognizes its professional obligation to safeguard and improve the welfare of laboratory animals. This module outlines the regulation and implementation of guidelines that must be followed when laboratory animals are used.  Assignment 1) The Animal Welfare Act was enacted in 1966 and has been amended by Congress four times. The Act regulates humane treatment of animals at all research facilities-public or private, academic or industry-based, whether or not they receive federal funds. All covered research facilities must register with the U.S. Department of Agriculture and comply with USDA animal welfare regulations and standards. The Animal Welfare Act is designated for guinea pigs, hamsters, gerbils, rabbits, dogs, cats, nonhuman primates, marine mammals, farm animal species, excluding rats, mice and birds. Go to HYPERLINK "http://www.nabranimallaw.org/"http://www.nabranimallaw.org/ and identify the act passed by Congress which created a federal mandate governing the use of animals including rats and mice. Go to HYPERLINK "http://grants.nih.gov/grants/olaw/references/phspol.htm"http://grants.nih.gov/grants/olaw/references/phspol.htm and determine the NIH office responsible for the administration and coordination outlined in the Public Health Service Policy. 2) Animal Welfare Regulations require that all federally funded research facilities using animals establish an Institutional Animal Care and Use Committee (IACUC) to oversee animal use and assure compliance with all applicable laws and regulations. A primary responsibility of the IACUC is to review and approve all proposed activities involving the care and use of animals used in research, testing or teaching procedures, as well as review and approve all significant changes to ongoing activities. The IACUC monitors the animal care and use program by conducting thorough reviews and inspections of the animal facilities every six months. The IACUC submits documentation of its program evaluation and facility inspections to the Office of Laboratory Animal Welfare. Go to HYPERLINK "http://grants.nih.gov/grants/olaw/tutorial/iacuc.htm"http://grants.nih.gov/grants/olaw/tutorial/iacuc.htm and identify the required members of every IACUC committee. Go to HYPERLINK "http://grants.nih.gov/grants/olaw/tutorial/animal.htm"http://grants.nih.gov/grants/olaw/tutorial/animal.htm and determine who appoints the members of an IACUC committee at each institution. 3) The scientific community founded a number of organizations to improve laboratory animal care. The American Association for Laboratory Animal Science (AALAS) is one of the most prominent organizations and offers a variety of resources for the education and advancement of responsible laboratory animal care and use. Go to HYPERLINK "https://www.aalaslearninglibrary.org"https://www.aalaslearninglibrary.org and browse the resources available for professionals involved in animal research. You are going to complete an AALAS tutorial and answer questions based on the information in the tutorial. To log in to the tutorial: Click on Animal Care and Use Courses Click on Free Courses Click on Working with the IACUC Click on Working with the IACUC: non-VA version Click on Course Click on Continue Without Logging In Browse the 25 slides and answer the following questions: Describe the basic function of an IACUC. What is the central theme in selecting an animal model for research? What are the important points in justifying the number of animals needed for a research project? Animal Welfare Regulations require that investigators proposing research or testing on animals that may cause more than slight or momentary pain or distress to consider alternatives to such procedures. Identify the three Rs of animal research. Investigators must also provide a written narrative describing the methods and sources used to determine that alternatives are not available. List the databases available for searching for alternatives. Describe the four pain/distress categories mandated by the USDA. Investigators wishing to perform surgery on research animals must satisfy a number of requirements prior to IACUC approval. List three topics that must be addressed in a surgery protocol. Define major surgery. List the justifications for multiple survival surgeries. In accordance with the Occupation Health and Safety guidelines, the principal investigator of a research project is responsible for providing training for staff that work with the animals. List the four areas of training required. Describe the difference between physical and non-physical methods of euthanasia. If you observe misuse or mistreatment of animals, or you see procedures that you dont think comply with federal regulations or guidelines, you should immediately report the violations to the IACUC at your facility. If you are not satisfied with the actions taken by your local IACUC, list two federal agencies you should contact. Why is animal research necessary? 4) In 1955 the National Institutes of Health (NIH) published the first federal laboratory animal guidelines, now entitled the Guide for the Care and Use of Laboratory Animals. The Guide describes institutional responsibilities and professional standards to ensure animals are cared for in ways that are scientifically, technically, and humanely appropriate. Go to HYPERLINK "http://www.aaalac.org/resources/theguide.cfm"http://www.aaalac.org/resources/theguide.cfm and browse through the full text copy of the Guide. Chapter 2 describes animal environment, housing and management. List the general recommendations for a 10 g mouse for each of the following factors: space temperature humidity ventilation illumination noise activity food water bedding sanitation +! 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