ࡱ> ]_\ r+bjbjVV :P<<.#>  II8Qe0X0Z0Z0Z0Z0Z0Z0_25lZ0Z0IIo0IX0X00.6 0~.D0000.6m5~.m5, 0m5 0$Z0Z00m5 : Section Animal Science Unit Unit 6 Reproduction and Genetics Lesson Title Lesson 19 - Embryo Transfer Student Learning Objectives As a result of this lesson, the student will: Be able to define embryo transfer. Be able to describe how the embryo transfer process is done. Analyze the benefits and limitations of utilizing embryo transfer. Time Instruction time for this lesson: 50 minutes. Resources Scientific Farm Animal Production, Robert E. Taylor Biology The Dynamics of Life, Biggs, Kapicka, and Lundgren  HYPERLINK "http://www.agtechinc.com/overview/" http://www.agtechinc.com/overview/ http://www.etschool.com/ Tools, Equipment, and Supplies Overhead Projector/Computer with Projector 1 Copy Per Student of Embryo Transfer Quiz Transparencies or PowerPoint of slides 2-6 5 Jelly Beans Per group of 3 5 Gummy Worms Per group of 3 5 Cheap White Paper Plates Per group of 3 1 Nice Quality Colored paper plate Per group of 3 Key Terms Embryo Transfer Super Ovulation Interest Approach Divide your class into groups of three and give each group 5 jelly beans, 5 gummy worms, 5 cheap white paper plates and one higher quality colored paper plate. You are going to have them imagine that all 6 paper plates represent the reproductive tracts of 6 different cows. The jelly beans are to be eggs and the worms are to be sperm. You will have the group put all of the eggs into the higher quality colored paper plate and then have the sperm unite with the eggs. Once embryos are formed you are going to move the entire embryo from the one cow to the other 5 recipient cows, leaving the donor with no embryos. This demonstrates very well how embryo transfer works in real life. Hello class! Today we are going to introduce our lesson with an activity. I am going to divide you into groups of three. After I pick the groups I will say go. You then have 10 seconds to find a seat with your groups and pay attention to the rest of the directions. Pick Groups. GO. O.K. Here are the rest of the directions. I am passing out to each group 5 jelly beans, 5 gummy worms, 5 white plates and 1 colored plate. Once you have all of your materials, I want you to spread your plates out and pretend that each plate represents a reproductive tract of 6 different cows. Does everyone have their plates spread out? Good. Now place all 5 jelly beans in the one cow represented by the colored plate and pretend that these are all eggs. Does everyone have their eggs in one cow? Great, now fertilize your eggs in this one cow with your gummy worm sperm. Now that your eggs are fertilized take each embryo and implant it in a different recipient cow, leaving your donor cow with no embryos. This means you should have one egg and sperm in 5 different plates, leaving the original plate empty. Does anyone know what this activity represents? Correct, embryo transfer. That leads us to todays findings. Do not eat your project yet, as we will be using it later. Summary of Content and Teaching Strategies Objective 1. Be able to define what embryo transfer is. Show slides 2-3. Have students record their knowledge on the Basics of Embryo Transfer notes page throughout the day. What is embryo transfer? Embryo Transfer This procedure is when an embryo in its early development is transferred from its own mother to a recipient mother. Genetic progress is achieved fairly quickly with artificial insemination AI limits progress to male side of genetic contribution Embryo transfer allows each a cow to produce many offspring and offers more rapid genetic gain. Great! Now that you all know what embryo transfer is lets find out how it is done. Objective 2. Be able to describe how the embryo transfer process is done. Show slides 4-5 How is embryo transfer done? The process of cattle embryo transfer begins by selecting a genetically outstanding, well-nourished, non pregnant embryo donor (heifer or cow). At the same time, 10 to 15 non pregnant females (embryo recipients) are identified. Super ovulation The donor cow is super ovulated by using injectable hormones. An average donor in cattle produces 6 transferable embryos. However 0-25 embryos can be expected. Insemination Cattle that are super ovulated are heat detected and inseminated at both 12 and 24 hours after heat with two straws of semen. After 7 days, the embryos have developed into a transferable state. When the 7 days are up, a special solution is pushed into the reproductive tract of the donor and the embryos are washed out of her. The solution that comes out of the tract is taken into a laboratory and searched for embryos. The embryos can be freshly transferred into another cow that is in the same part of her cycle as the donor or they can be frozen. Now that you all have learned the embryo transfer process, when I say go, I want you to get back in your original groups and go through the process with the supplies I have given you. I will come around and have each group demonstrate embryo transfer to me step by step. Go, you have 3 minutes before I will evaluate your jellybean and gummy worm embryo transfer. Evaluate each group on the 7 steps listed above and answer any questions. Good, everyone did excellent! Objective 3. Analyze the benefits and limitations of utilizing embryo transfer. Show slides 6-8 and remind students to record information on Basics of Embryo Transfer notes page. What are the benefits of embryo transfer? To produce more offspring from a valuable dam. Some infertility problems can be avoided. Prevents disease transmission Reproduce some endangered species faster than normally possible. What are the limitations of embryo transfer? Decreases genetic diversity Decreased diversity often leads to mutations Expensive and time consuming procedure Who is this practical for? Seed stock producers An animal of genetic superiority whose offspring is demanded by the industry. Review/Summary. For your assignment for today I want you to summarize todays findings by making a brochure on embryo transfer. I want you to pretend that you are a company specializing in embryo transfer. Make sure you include a description on what embryo transfer is, the procedure that you will use, and why a producer might want to utilize embryo transfer. You will be graded individually on this project. You have the rest of todays period. The rest of the assignment will be completed for homework. Application Extended classroom activity The brochure activity used in the review. FFA activity On an FFA trip schedule a tour at a facility specializing in embryo transfer. SAE activity Have students evaluate the best animal in their herd and describe why they would like more calves out of her if embryo transfer technology was more feasible. Evaluation Students should be complete the embryo transfer quiz. Answers to Assessment: F F T T T F F T T T Embryo Transfer Quiz True and False. Write T next to true statement and write F next to false statements. 1._______Embryo Transfer is a process in which you transfer eggs into a donor to later be fertilized. 2._______Embryo Transfer is very cost effective. 3._______The first step in embryo transfer is super ovulating the donor cow. 4._______You should expect 6 transferable embryos from the donor cow. 5._______Seedstock producers often use embryo transfer. 6._______Commercial cow calf producers often use embryo transfer. 7._______Superovulated cows are bred naturally. 8._______Cows are super ovulated by injecting them with hormones. 9._______1 cow can mother several calves in one year utilizing embryo transfer. 10.______The embryos are born and taken care of by a recipient animal. The Basics of Embryo Transfer Define embryo transfer: Record the process of embryo transfer in the following table Step Draw a picture here that represents this step.Step Description1The process of cattle embryo transfer begins by selecting a genetically outstanding, well-nourished, non pregnant embryo donor (heifer or cow). At the same time, 10 to 15 non pregnant females (embryo recipients) are identified.2Super ovulation The donor cow is super ovulated by using injectable hormones. An average donor in cattle produces 6 transferable embryos. However 0-25 embryos can be expected. 3Insemination Cattle that are super ovulated are heat detected and inseminated at both 12 and 24 hours after heat with two straws of semen.4After 7 days, the embryos have developed into a transferable state.5When the 7 days are up, a special solution is pushed into the reproductive tract of the donor and the embryos are washed out of her.6The solution that comes out of the tract is taken into a laboratory and searched for embryos.7The embryos can be freshly transferred into another cow that is in the same part of her cycle as the donor or they can be frozen.  Benefits: Limitations: Who can utilize this technology?     Unit 4, Lesson 19: Embryo Transfer  PAGE 1  $%BCQ] a < = > ` a y2|SRcb!~!!!!"""""" ########h 7hw5hwB*ph hw56 hw6 hw0JjhwUjhwU hw0J hK5 hw5hwHBCmn \ ] b { |   & F & F & Fh^h & F p  ; Z 67 & FA"#xy]@ & F & F^Z^Z & F & F & F@12|}ST~6cRS & F & F & Fh^h^ & FScS!T!a!b!~!!!!"""""""" # ######### & F^`##########!#6#9####K&i&''''''(*.+/+1+2+4+5+7+8+:+_+`+f+g+h+i+k+l+r+jhw0JU*hK0JmHnHu* hw0Jjhw0JUh`jh`Uhw56CJhwB*CJph hwCJ hw56 hw6hw hw5+## #6#7#8#9########+$,$y$z$$$$$<%=%m%n%%% ^`$a$ & F%&&I&J&i&&&&&&'$If$a$ ''' ''qkaa $If^$Ifkd$$IflF$Jx t06    44 la''''((qkkak $If^$Ifkd$$IflF$Jx t06    44 la((((8)qkka $If^$IfkdQ$$IflF$Jx t06    44 la8)9);)<))qkka $If^$Ifkd$$IflF$Jx t06    44 la)))) *qkkk$Ifkd$$IflF$Jx t06    44 la * * * *k*qkka $If^$IfkdI$$IflF$Jx t06    44 lak*l*n*o***qkkak $If^$Ifkd$$IflF$Jx t06    44 la***** + +.+0+1+3+4+6+qoooooooooookd$$IflF$Jx t06    44 la 6+7+9+:+j+k+l+m+n+o+p+q+r+ 8 00P/ =!"#$% DpDyK #http://www.agtechinc.com/overview/yK Fhttp://www.agtechinc.com/overview/$$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x $$If!vh5J55x #vJ#v#vx :V l t065J55x ^ 666666666vvvvvvvvv666666>6666666666666666666666666666666666666666666666666hH6666666666666666666666666666666666666666666666666666666666666666666866666662 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p(8HX`~_HmH nH sH tH L`L Normal1$7$8$H$CJ_HaJmH sH tH :@:  Heading 1$@&5\VV  Heading 3$<@&5CJOJQJ\^JaJDA`D Default Paragraph FontVi@V 0 Table Normal :V 44 la (k ( 0No List <& < Footnote ReferenceB'B Comment ReferenceCJaJ<<  Comment TextCJaJ@j@ Comment Subject5\H2H  Balloon TextCJOJQJ^JaJNOAN Char Char!5CJ\_HaJmH sH tH uDRD Direction Text ^60Ob0 Script ^6U@q6  Hyperlink >*B*ph44 Header  !4 @4 Footer  !.)@.  Page NumberPK![Content_Types].xmlj0Eжr(΢Iw},-j4 wP-t#bΙ{UTU^hd}㨫)*1P' ^W0)T9<l#$yi};~@(Hu* Dנz/0ǰ $ X3aZ,D0j~3߶b~i>3\`?/[G\!-Rk.sԻ..a濭?PK!֧6 _rels/.relsj0 }Q%v/C/}(h"O = C?hv=Ʌ%[xp{۵_Pѣ<1H0ORBdJE4b$q_6LR7`0̞O,En7Lib/SeеPK!kytheme/theme/themeManager.xml M @}w7c(EbˮCAǠҟ7՛K Y, e.|,H,lxɴIsQ}#Ր ֵ+!,^$j=GW)E+& 8PK!Ptheme/theme/theme1.xmlYOo6w toc'vuر-MniP@I}úama[إ4:lЯGRX^6؊>$ !)O^rC$y@/yH*񄴽)޵߻UDb`}"qۋJחX^)I`nEp)liV[]1M<OP6r=zgbIguSebORD۫qu gZo~ٺlAplxpT0+[}`jzAV2Fi@qv֬5\|ʜ̭NleXdsjcs7f W+Ն7`g ȘJj|h(KD- dXiJ؇(x$( :;˹! I_TS 1?E??ZBΪmU/?~xY'y5g&΋/ɋ>GMGeD3Vq%'#q$8K)fw9:ĵ x}rxwr:\TZaG*y8IjbRc|XŻǿI u3KGnD1NIBs RuK>V.EL+M2#'fi ~V vl{u8zH *:(W☕ ~JTe\O*tHGHY}KNP*ݾ˦TѼ9/#A7qZ$*c?qUnwN%Oi4 =3ڗP 1Pm \\9Mؓ2aD];Yt\[x]}Wr|]g- eW )6-rCSj id DЇAΜIqbJ#x꺃 6k#ASh&ʌt(Q%p%m&]caSl=X\P1Mh9MVdDAaVB[݈fJíP|8 քAV^f Hn- "d>znNJ ة>b&2vKyϼD:,AGm\nziÙ.uχYC6OMf3or$5NHT[XF64T,ќM0E)`#5XY`פ;%1U٥m;R>QD DcpU'&LE/pm%]8firS4d 7y\`JnίI R3U~7+׸#m qBiDi*L69mY&iHE=(K&N!V.KeLDĕ{D vEꦚdeNƟe(MN9ߜR6&3(a/DUz<{ˊYȳV)9Z[4^n5!J?Q3eBoCM m<.vpIYfZY_p[=al-Y}Nc͙ŋ4vfavl'SA8|*u{-ߟ0%M07%<ҍPK! ѐ'theme/theme/_rels/themeManager.xml.relsM 0wooӺ&݈Э5 6?$Q ,.aic21h:qm@RN;d`o7gK(M&$R(.1r'JЊT8V"AȻHu}|$b{P8g/]QAsم(#L[PK-![Content_Types].xmlPK-!֧6 +_rels/.relsPK-!kytheme/theme/themeManager.xmlPK-!Ptheme/theme/theme1.xmlPK-! ѐ' theme/theme/_rels/themeManager.xml.relsPK] r#PPPPPr#P ===@#r+ @S#%''(8)) *k**6+r+ !"#$%&' =`r#X18:@!8@0(  B S  ?r#$% a.#.#0#1#1#3#4#6#7#9#:#_#i#k#l#p#s#$%-#.#_#i#s#5Df2ܰ d?*["H.?B`86`ʰ*]th( j{ih Z\Th^`OJQJo(hHh^`OJQJ^Jo(hHohpp^p`OJQJo(hHh@ @ ^@ `OJQJo(hHh^`OJQJ^Jo(hHoh^`OJQJo(hHh^`OJQJo(hHh^`OJQJ^Jo(hHohPP^P`OJQJo(hH  ^ `o(. xx^x`hH. HLH^H`LhH. ^`hH. ^`hH. L^`LhH. ^`hH. X X ^X `hH. (#L(#^(#`LhH.^`o(. ^`hH. pLp^p`LhH. @ @ ^@ `hH. ^`hH. L^`LhH. ^`hH. ^`hH. PLP^P`LhH.0^`0o(.^`o(.  ^ `o(.   ^ `hH. xx^x`hH. HLH^H`LhH. ^`hH. ^`hH. L^`LhH.^`o(.   ^ `hH.  L ^ `LhH. xx^x`hH. HH^H`hH. L^`LhH. ^`hH. ^`hH. L^`LhH.  ^ `o(. xx^x`hH. HLH^H`LhH. ^`hH. ^`hH. L^`LhH. ^`hH. X X ^X `hH. (#L(#^(#`LhH.^`o(. ^`hH. pLp^p`LhH. @ @ ^@ `hH. ^`hH. L^`LhH. ^`hH. ^`hH. PLP^P`LhH.h^`OJQJo(hHh^`OJQJ^Jo(hHohpp^p`OJQJo(hHh@ @ ^@ `OJQJo(hHh^`OJQJ^Jo(hHoh^`OJQJo(hHh^`OJQJo(hHh^`OJQJ^Jo(hHohPP^P`OJQJo(hHiB86`th2?*["5          <        -        v.dF}Њ1      n-        X        -                 `Kw.#0#@r#p@UnknownG* Times New Roman5Symbol3. * Arial5. *aTahoma?= * Courier New;WingdingsACambria Math" h;F; c@?@?! xxd### 2Q HX?' 72Lesson Kim RomineComputer Services,        Oh+'0 $ D P \ ht|Lesson Kim Romine Normal.dotmComputer Services11Microsoft Office Word@ @@6@&8a@՜.+,D՜.+,< hp   CAERT, Inc.?# Lesson Title 8@ _PID_HLINKSA o#http://www.agtechinc.com/overview/  !"#$%&'(*+,-./023456789:;<=>?@ABCDEFGHIJKMNOPQRSUVWXYZ[^Root Entry FN `Data )1Table15WordDocument:PSummaryInformation(LDocumentSummaryInformation8TCompObjy  F'Microsoft Office Word 97-2003 Document MSWordDocWord.Document.89q