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5 : Below you will find objectives, a problem set and suggested extra material. Record work on objectives as you read and review information. You can find information online, in books, from us or from each other. Look for reliable sources; see suggested references below. Problem set questions ask you to utilize knowledge and often, integrate multiple concepts.
Objectives: Write out material in your course notebook.
Membrane properties
List the basic components of a cell membrane.
Explain how the composition of a cell membrane influences the membrane permeability of different types of molecules.
List basic membrane protein types and their function. (Access multiple sources as they may categorize membrane protein groups differently.)
Membrane transport
[Optional: Review principles of diffusion, osmosis, and ATP-mediated transport. Tutorials can be found at HYPERLINK "http://wps.aw.com/bc_garrett_getready_1/68/17637/4515324.cw/index.html" http://wps.aw.com/bc_garrett_getready_1/68/17637/4515324.cw/index.html ]
Define properties, state source(s) of energy for transport and give a specific example for each of the following membrane transport protein types:
Channel protein
Carrier protein
Facilitated diffusion carrier
Primary active transporter
Secondary active transporter
Distinguish between endocytosis and exocytosis. List of diagram the steps for exocytosis. (There are several molecules identified in the process, such a calmodulin and SNAREs.)
List the normal range for extracellular fluid Na+, K+, H+ (pH), HCO3-, Cl-, Ca2+ & glucose.
List the normal range for intracellular Na+, K+, H+ (pH), HCO3-, Cl-, Ca2+.
State the method(s) of movement across a cell membrane for each of the following: If there are multiple methods, list as many as you can find.
Oxygen
Carbon dioxide
Calcium
Sodium
Glucose
Insulin (a protein)
Testosterone (a lipid)
Water
Define saturation, competition, and specificity for a transport or receptor protein.
Problem Set: Write out answers in your course notebook. A word file can also be accessed at HYPERLINK "http://helios.hampshire.edu/~cjgNS/sputtbug/416l/HumanPhys.html" http://helios.hampshire.edu/~cjgNS/sputtbug/416l/HumanPhys.html
In controlled lab conditions, pure phospholipid bilayers have been created. Living plant and animal cells have phospholipid bilayer membranes with many additional lipids, proteins and carbohydrates added. Describe the functions that the pure phospholipid bilayer would share with a living cell.
Some proteins span the membrane (from extracellular to cytosolic surfaces) while others are restricted to one surface. Hypothesize about the general functions of integral (transmembrane) versus extracellular surface versus cytosolic surface proteins.
For each of the following conditions, circle the answer in italics that would result in increased rate of oxygen simple diffusion into cells: (ICF= intracellular fluid; ECF= extracellular fluid)
oxygen levels: high ICF [oxygen] or low ICF [oxygen] or no effect
sodium levels: high ICF[ Na+] or low ICF [Na+] or no effect
carbon dioxide levels: high ICF [CO2] or low ICF [CO2] or no effect
Membrane channels can be gated to restrict transport under specific conditions. Explain briefly how each of the following channels type is opened in a living system: mechanically-gated channel, chemically-gated channel and voltage gated channel. (Yea, yea you could say, mechanically, chemically and with voltage, but this question is asking you to put into your own words what is inducing each type of channel to open. There are many ways to describe this.)
The following graph depicts the distribution of some important solutes in body fluids. Note that the extracellular fluid compartments include the plasma and interstitial fluids. Redraw the graph to have plasma, interstitial fluid and ICF as the major independent axis groups, and solutes then as subgroups. Postulate then on why sodium (Na+) is so often part of co-transporter mechanisms. And also postulate on why large anions could not be. If you are really, really clever and know something about electrical properties of cells, you can also explain why K+ is not commonly part of co-transporter mechanisms like sodium.
The kidney tissue diagrammed below is specialized to assist with regulation of Na+ and K+ ions in the body. Each cell transports K+ from the ECF to the lumen for excretion and transports Na+ from the kidney lumen to the ECF for retention in the body. This process requires three membrane transport protein types.
On a cell in the diagram below, add the three transport proteins that will allow the transport of K+ and Na+ ions as described. Identify for each what the protein transports and the source of energy for the transport. (Consider the normal concentrations of Na+ and K+ ions inside and outside cells, the types of transport proteins and their energy sources. Lumen ion concentrations vary widely.)
On the plot below, draw a simple graph to show how diffusion rate of glucose into muscle cells will change as the number of GLUT transporters increases. Label the variable for the X and Y axes.
EMBED Excel.Sheet.8
Explain (or diagram) how a mammary gland duct epithelial cell secrets milk. Note this is a difficult problem that includes the concept of transepithelial transport, with cell types that have functionally distinct surfaces.
State how saturation is affected by the presence of two ligands rather than one.
State how specificity will effect competition for two molecules with different affinities for the same receptor (or transporter).
Optional extra activities:
More review of active and passive transport mechansisms: HYPERLINK "http://www.northland.cc.mn.us/biology/Biology1111/animations/transport1.html" http://www.northland.cc.mn.us/biology/Biology1111/animations/transport1.html
Animations of neurotransmitter-activated ion channels actions at
HYPERLINK "http://www.blackwellpublishing.com/matthews/neurotrans.html" http://www.blackwellpublishing.com/matthews/neurotrans.html
The complete blood count (CBC) is a test used in health care to compare patient plasma components to establish average ranges. Abnormally high or low values may indicate the presence of disease, and variations from normal values can dramatically change cellular functions. The CBC is often ordered with a basic metabolic panel (BMP), a set of seven blood chemical tests (also called a CHEM-7). You can explore the CBC and more extensive CBC CHEM-7 test at HYPERLINK "http://medlineplus.gov/" http://medlineplus.gov/ . Consider how variations from normal ranges would affect transport and cell function.
Symptoms of hypercalemia (high ECF Ca2+ concentration). Consider mechanisms:
HYPERLINK "http://www.clevelandclinicmeded.com/medicalpubs/diseasemanagement/endocrinology/hypercalcemia/" http://www.clevelandclinicmeded.com/medicalpubs/diseasemanagement/endocrinology/hypercalcemia/
See more about transporters and interesting genetics at:
HYPERLINK "http://www.sciencedirect.com.silk.library.umass.edu:2048/science?_ob=RedirectURL&_method=externObjLink&_locator=url&_cdi=6245&_plusSign=%2B&_targetURL=http%253A%252F%252Fwww.med.rug.nl%252Fmdl%252Fhumanabc.htm" \t "externObjLink" http://www.med.rug.nl/mdl/humanabc.htm
HYPERLINK "http://www.sciencedirect.com.silk.library.umass.edu:2048/science?_ob=RedirectURL&_method=externObjLink&_locator=url&_cdi=6245&_plusSign=%2B&_targetURL=http%253A%252F%252Fwww.med.rug.nl%252Fmdl%252Ftab3.htm" \t "externObjLink" http://www.med.rug.nl/mdl/tab3.htm
HYPERLINK "http://www.sciencedirect.com.silk.library.umass.edu:2048/science?_ob=RedirectURL&_method=externObjLink&_locator=url&_cdi=6245&_plusSign=%2B&_targetURL=http%253A%252F%252Fmips.gsf.de%252Fproj%252Fyeast%252Fcatalogues%252Fprotein_classes%252Findex.html" \t "externObjLink" http://mips.gsf.de/proj/yeast/catalogues/protein_classes/index.html
Want more practice with the basic transport processes (diffusion, osmosis, active transport)? You can find many explanations and animations on the internet through a google search by term. You also can try a virtual experiment at HYPERLINK "http://www.PhysiologyPlace.com" www.PhysiologyPlace.com. Click on the Silverthorn, Human Physiology 4/E text. Log in with my name and password cgill42, poo8phys. Go to the Lab Simulation, Cell Transport Mechanisms and Permeability. Youll need shockwave for this to run. You can print the lab exercise pages and go through the simulation online. Consider though that a living cell is pliable and stretchable in a way not represented by static chambers in the simulation.]
Cell Biology Objectives and Worksheet 1: Cell Membranes and Transport
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