ࡱ> 5HJ !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFG L bjbjWW == d--///$000P^0, :0B cFccdW []]]]]]$2/ LW--cdi;2- c/d[[D/dMF ;GL<2 L/>,$J +:  December 2013 Teacher's Guide Table of Contents  TOC \o "1-3" \h \z \u  HYPERLINK \l "_Toc375043494" About the Guide  PAGEREF _Toc375043494 \h 3  HYPERLINK \l "_Toc375043495" Student Questions (from the articles)  PAGEREF _Toc375043495 \h 4  HYPERLINK \l "_Toc375043496" Answers to Student Questions (from the articles)  PAGEREF _Toc375043496 \h 6  HYPERLINK \l "_Toc375043497" ChemMatters Puzzle: An Element Fill-In Puzzle  PAGEREF _Toc375043497 \h 11  HYPERLINK \l "_Toc375043498" Answers to the ChemMatters Puzzle  PAGEREF _Toc375043498 \h 12  HYPERLINK \l "_Toc375043499" National Science Education Standards (NSES) Correlations  PAGEREF _Toc375043499 \h 13  HYPERLINK \l "_Toc375043500" Next-Generation Science Standards (NGSS) Correlations  PAGEREF _Toc375043500 \h 15  HYPERLINK \l "_Toc375043501" Common Core State Standards Connections  PAGEREF _Toc375043501 \h 17  HYPERLINK \l "_Toc375043502" Anticipation Guides  PAGEREF _Toc375043502 \h 18  HYPERLINK \l "_Toc375043503" Hot Peppers: Muy Caliente!  PAGEREF _Toc375043503 \h 19  HYPERLINK \l "_Toc375043504" Global Climate Change: A Reality Check  PAGEREF _Toc375043504 \h 20  HYPERLINK \l "_Toc375043505" Peering through Urine  PAGEREF _Toc375043505 \h 21  HYPERLINK \l "_Toc375043506" Morphine & Heroin: The Yin & Yang of Narcotics  PAGEREF _Toc375043506 \h 22  HYPERLINK \l "_Toc375043507" Opals: Natures Colorful Gemstones  PAGEREF _Toc375043507 \h 23  HYPERLINK \l "_Toc375043508" Reading Strategies  PAGEREF _Toc375043508 \h 24  HYPERLINK \l "_Toc375043509" Hot Peppers: Muy Caliente!  PAGEREF _Toc375043509 \h 25  HYPERLINK \l "_Toc375043510" Global Climate Change: A Reality Check  PAGEREF _Toc375043510 \h 26  HYPERLINK \l "_Toc375043511" Peering through Urine  PAGEREF _Toc375043511 \h 27  HYPERLINK \l "_Toc375043512" Morphine & Heroin: The Yin & Yang of Narcotics  PAGEREF _Toc375043512 \h 28  HYPERLINK \l "_Toc375043513" Opals: Natures Colorful Gemstones  PAGEREF _Toc375043513 \h 29  HYPERLINK \l "_Toc375043514" Hot Peppers: Muy Caliente!  PAGEREF _Toc375043514 \h 30  HYPERLINK \l "_Toc375043515" Background Information (teacher information)  PAGEREF _Toc375043515 \h 30  HYPERLINK \l "_Toc375043516" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc375043516 \h 41  HYPERLINK \l "_Toc375043517" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc375043517 \h 42  HYPERLINK \l "_Toc375043518" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc375043518 \h 42  HYPERLINK \l "_Toc375043519" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc375043519 \h 42  HYPERLINK \l "_Toc375043520" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc375043520 \h 43  HYPERLINK \l "_Toc375043521" References (non-Web-based information sources)  PAGEREF _Toc375043521 \h 44  HYPERLINK \l "_Toc375043522" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc375043522 \h 44  HYPERLINK \l "_Toc375043523" More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers)  PAGEREF _Toc375043523 \h 45  HYPERLINK \l "_Toc375043524" Global Climate Change: A Reality Check  PAGEREF _Toc375043524 \h 46  HYPERLINK \l "_Toc375043525" Background Information (teacher information)  PAGEREF _Toc375043525 \h 46  HYPERLINK \l "_Toc375043526" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc375043526 \h 65  HYPERLINK \l "_Toc375043527" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc375043527 \h 65  HYPERLINK \l "_Toc375043528" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc375043528 \h 66  HYPERLINK \l "_Toc375043529" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc375043529 \h 67  HYPERLINK \l "_Toc375043530" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc375043530 \h 68  HYPERLINK \l "_Toc375043531" References (non-Web-based information sources)  PAGEREF _Toc375043531 \h 69  HYPERLINK \l "_Toc375043532" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc375043532 \h 69  HYPERLINK \l "_Toc375043533" Peering through Urine  PAGEREF _Toc375043533 \h 72  HYPERLINK \l "_Toc375043534" Background Information (teacher information)  PAGEREF _Toc375043534 \h 72  HYPERLINK \l "_Toc375043535" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc375043535 \h 78  HYPERLINK \l "_Toc375043536" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc375043536 \h 79  HYPERLINK \l "_Toc375043537" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc375043537 \h 79  HYPERLINK \l "_Toc375043538" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc375043538 \h 80  HYPERLINK \l "_Toc375043539" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc375043539 \h 80  HYPERLINK \l "_Toc375043540" References (non-Web-based information sources)  PAGEREF _Toc375043540 \h 81  HYPERLINK \l "_Toc375043541" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc375043541 \h 81  HYPERLINK \l "_Toc375043542" More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers)  PAGEREF _Toc375043542 \h 83  HYPERLINK \l "_Toc375043543" Morphine & Heroin: The Yin and Yang of Narcotics  PAGEREF _Toc375043543 \h 84  HYPERLINK \l "_Toc375043544" Background Information (teacher information)  PAGEREF _Toc375043544 \h 84  HYPERLINK \l "_Toc375043545" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc375043545 \h 91  HYPERLINK \l "_Toc375043546" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc375043546 \h 91  HYPERLINK \l "_Toc375043547" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc375043547 \h 92  HYPERLINK \l "_Toc375043548" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc375043548 \h 93  HYPERLINK \l "_Toc375043549" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc375043549 \h 94  HYPERLINK \l "_Toc375043550" References (non-Web-based information sources)  PAGEREF _Toc375043550 \h 94  HYPERLINK \l "_Toc375043551" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc375043551 \h 95  HYPERLINK \l "_Toc375043552" Opals: Playing with Color and Light  PAGEREF _Toc375043552 \h 97  HYPERLINK \l "_Toc375043553" Background Information (teacher information)  PAGEREF _Toc375043553 \h 97  HYPERLINK \l "_Toc375043554" Connections to Chemistry Concepts (for correlation to course curriculum)  PAGEREF _Toc375043554 \h 121  HYPERLINK \l "_Toc375043555" Possible Student Misconceptions (to aid teacher in addressing misconceptions)  PAGEREF _Toc375043555 \h 121  HYPERLINK \l "_Toc375043556" Anticipating Student Questions (answers to questions students might ask in class)  PAGEREF _Toc375043556 \h 122  HYPERLINK \l "_Toc375043557" In-class Activities (lesson ideas, including labs & demonstrations)  PAGEREF _Toc375043557 \h 122  HYPERLINK \l "_Toc375043558" Out-of-class Activities and Projects (student research, class projects)  PAGEREF _Toc375043558 \h 123  HYPERLINK \l "_Toc375043559" References (non-Web-based information sources)  PAGEREF _Toc375043559 \h 124  HYPERLINK \l "_Toc375043560" Web sites for Additional Information (Web-based information sources)  PAGEREF _Toc375043560 \h 125  HYPERLINK \l "_Toc375043561" More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers)  PAGEREF _Toc375043561 \h 130  HYPERLINK \l "_Toc375043562" General Web References (Web information not solely related to article topic)  PAGEREF _Toc375043562 \h 131  About the Guide Teachers Guide editors William Bleam, Donald McKinney, Ronald Tempest, and Erica K. Jacobsen created the Teachers Guide article material. E-mail:  HYPERLINK "mailto:bbleam@verizon.net" bbleam@verizon.net Susan Cooper prepared the national science education content, anticipation guides, and reading guides. David Olney created the puzzle. E-mail:  HYPERLINK "mailto:djolney@verizon.net" djolney@verizon.net Patrice Pages, ChemMatters editor, coordinated production and prepared the Microsoft Word and PDF versions of the Teachers Guide. E-mail:  HYPERLINK "mailto:chemmatters@acs.org" chemmatters@acs.org Articles from past issues of ChemMatters can be accessed from a CD that is available from the American Chemical Society for $30. The CD contains all ChemMatters issues from February 1983 to April 2008. The ChemMatters CD includes an Index that covers all issues from February 1983 to April 2008. The ChemMatters CD can be purchased by calling 1-800-227-5558. Purchase information can be found online at  HYPERLINK "http://chemistry.org/chemmatters/cd3.html" www.acs.org/chemmatters Student Questions (from the articles) Hot Peppers: Muy Caliente! Describe some of the effects of eating a Moruga Scorpion, one of the hottest chili peppers in the world. What compound causes the hot taste we experience when eating chili peppers? How is the hotness of a chili pepper measured? Discuss why capsaicins structure makes water a poor choice to cool the heat of a chili pepper in your mouth. What are some preferred foods/drinks to cool the heat of a chili pepper in your mouth? Why? How do chili peppers generate the feeling of heat without actually increasing the temperature of your tongue, mouth, and throat? Describe what happens when a capsaicin molecule bonds to a pain receptor. What happens in the body when capsaicin is applied to skin as a pain reliever? Global Climate Change: A Reality Check According to the article how much has the Earths average surface temperature increased in the last century? The article mentions an 8 inch rise in sea level since 1870. What two climate change factors caused the sea level increase? Why is 1870 an important time period in any discussion of climate change? List five changes brought about by climate change. List the gases that trap heat in the Earths atmosphere. What is the greenhouse effect? Describe how scientists know what the Earths climate was like thousands of years ago, before recorded temperatures? What is Global Warming Potential (GWP)? Besides GWP, what other factor helps to predict the overall effect of a greenhouse gas? What is carbon sequestration? Peering through Urine On average, how much urine does a person produce in a day? What purpose does urine serve for the body? What typically makes up a urine solution? What are some factors that can affect the color of your urine? List several physical properties of urine that can be observed when analyzing urine for potential signs of sickness. What is one of the most common ways to detect chemicals in urine? List several substances that one can test for in the urine using a dipstick. How is a dipstick able to indicate differing amounts of certain substances in your urine? Morphine & Heroin: The Yin and Yang of Narcotics What is the source of morphine? How does opium differ from morphine? Heroin is chemically derived from morphine. Describe the chemical reaction that converts morphine to heroin. What happens to heroin, chemically, when it enters the brain? What is an endorphin? In what way is morphine like endorphins? What is meant by drug tolerance? Why is heroin usually injected directly into the bloodstream, instead of being taken by mouth as is done with morphine? What is dopamine? Why is morphine called a two-edged sword? Opals: Playing with Color and Light Name the phenomenon that only opals, among all gemstones, display. How many silicon atoms surround each oxygen atom in the silica crystal? How are sand and opal alike? How are they different? What special characteristic of the structure of precious opal gives it its characteristic play of color? What is the name of the process that occurs when light is bent? Why do different colors appear inside an opal? What two conditions are needed for opals to form? What is the formula for opal? What practical application has been made for synthetic polymer opals? How do these polymer films work? Answers to Student Questions (from the articles) Hot Peppers: Muy Caliente! Describe some of the effects of eating a Moruga Scorpion, one of the hottest chili peppers in the world. Some of the effects of eating one of the hottest chili peppers in the world are: Pain Mouth, tongue, and throat feel like they are on fire Eyes water Ears ring Lips go numb Face turns red Sweat profusely What compound causes the hot taste we experience when eating chili peppers? The compound that causes the hot taste is capsaicin, a colorless, odorless oil-like compound. How is the hotness of a chili pepper measured? The hotness of a chili pepper is measured by the Scoville heat scale, which is a series of heat units that range from 0 (bell pepper) to 16 million (pure capsaicin), depending on the peppers capsaicin content. Discuss why capsaicins structure makes water a poor choice to cool the heat of a chili pepper in your mouth. Capsaicins structure has a long hydrocarbon tail. Capsaicin ends up being nonpolar overall because of its molecular structure, especially the tail. Water is an example of a polar molecule. When you drink water after eating a chili pepper (nonpolar), the water (polar) just moves more of it around your mouth, making the pain worse. What are some preferred foods/drinks to cool the heat of a chili pepper in your mouth? Why? Some preferred foods/drinks are milk, ice cream, and bread. Milk and ice cream contain nonpolar molecules called casein. Casein molecules attract capsaicin molecules. They surround the capsaicin molecules and wash them away. Bread or other starchy foods made of nonpolar molecules can also help. How do chili peppers generate the feeling of heat without actually increasing the temperature of your tongue, mouth, and throat? Chili peppers generate the feeling of heat by triggering pain receptors in your tongue, mouth, and throat that send a signal to the brain, which is interpreted as heat. Capsaicin also stimulates receptors that perceive heat, known as thermoreceptors. Describe what happens when a capsaicin molecule bonds to a pain receptor. When a capsaicin molecule bonds to a pain receptor, calcium ions flood in. This triggers the release of neurotransmitters (chemicals that are transmitted from one neuron to the next) that send a message to the brain. The brain interprets this message as pain. What happens in the body when capsaicin is applied to skin as a pain reliever? When capsaicin is applied to skin, a steady stream of neurotransmitters is sent to the brain, stimulating pain signals in the body. Once these neurotransmitters are depleted, you no longer experience pain. You are exchanging short-lived intense pain for constant, low-level pain that your body gets used to. Global Climate Change: A Reality Check According to the article how much has the Earths average surface temperature increased in the last century? Most climate change experts agree that the increase in the Earths temperature in the last century is 0.8 oC (1.44 oF) The article mentions an 8 inch rise in sea level since 1870. What two climate change factors caused the sea level increase? The primary factor is the 0.8 oC increase in temperature of the atmosphere, which has caused the polar icebergs to melt, thus increasing sea levels globally. But the other factor is that the increase in temperature also causes the water in the oceans to expand, further increasing it volume. Why is 1870 an important time period in any discussion of climate change? In citing human involvement in global warming and climate change, it is often noted that industrial processes that increased emissions of carbon dioxide into the atmosphere are causative factors. By the 1870s most of the major technological changes that enabled these industrial processeslike mass production, increased fossil fuel production and use, railroad expansion and similar large-scale processes that increased CO2 emissionwere in place. Most of these processes involved the burning of fossil fuels. So by the 1870s many of the processes that now result in carbon dioxide emissions were well under way. List five changes brought about by climate change. In addition to atmospheric temperature increase and sea level rise caused by climate change, the article also mentions an increase in severe storms, hurricanes in new places around the globe, more frequent heat waves, changes in location of arable land, more wildfires and migration of animals to new habitats. List the gases that trap heat in the Earths atmosphere. The article lists carbon dioxide, nitrous oxide, methane and water vapor. What is the greenhouse effect? The greenhouse effect can be summarized this way: energy from the sun reaches the Earths surface mostly in the form of visible and ultraviolet radiation. The Earth absorbs that radiation and re-radiates it into the atmosphere in the form of infrared radiation (heat). Although oxygen and nitrogen, the primary constituent gases in the atmosphere, do not absorb this heat, other gases like carbon dioxide, methane, nitrous oxide and water vapor do absorb the heat, thus increasing the temperature of the atmosphere. This is the same way a greenhouse works. In a greenhouse the glass is transparent to incoming shorter wave length IR, visible and UV radiation but reflects the longer IR radiation that is radiated from the plants and soil in the greenhouse. Describe how scientists know what the Earths climate was like thousands of years ago, before recorded temperatures? There are a number of methods that are used, but the article describes the analysis of air bubbles trapped in ice core samples. The gases in the bubbles are analyzed and by looking at the resulting composition scientists know what gases existed in a given historical period. They can then relate this to the likely climate of the period. These methods are called analysis by proxy. What is Global Warming Potential (GWP)? Global Warming Potential is a number that is the ratio of the amount of infrared radiation absorbed by a given mass of a gas compared to the amount of infrared radiation absorbed by the same mass of carbon dioxide over the same time period. A number greater than 1 indicates that substance absorbs more IR than carbon dioxide (e.g., methanes 100-year GWP is 25, meaning it would absorb 25 times as much IR as a comparable mass of carbon dioxide over that 100 years). Besides GWP, what other factor helps to predict the overall effect of a greenhouse gas? The second important factor that helps predict the overall effect of a greenhouse gas is the length of time the gas stays in the atmosphere. Greenhouse gases remain in the atmosphere for different lengths of time. The shorter the length of time, the less that gass greenhouse impact. What is carbon sequestration? Carbon sequestration is a way of removing CO2 from the atmosphere. The article describes this method: CO2 is first compressed and then injected into porous rock at least 1 kilometer under the Earth. At that depth the gas remains compressed and will stay underground for long periods of time. Peering through Urine On average, how much urine does a person produce in a day? On an average day, a person can produce anywhere from half a liter to two liters of urine. What purpose does urine serve for the body? Urine is a way for the body to get rid of toxins and other harmful substances that build up in the blood. What typically makes up a urine solution? Urine is a solution that consists of 95% water and 5% organic solutes, including urea, creatinine, uric acid, plus inorganic ions such as sodium, potassium, and chloride. What are some factors that can affect the color of your urine? Some factors that can affect the color of urine are: Your level of hydration. Medications youre taking. Foods that you eat. Certain inherited diseases. List several physical properties of urine that can be observed when analyzing urine for potential signs of sickness. Some physical properties of urine that may be signs of sickness are: Cloudy urine can indicate a urinary tract infection or crystallized salts. Red or brown urine may contain blood, which can indicate dehydration, hepatitis B, kidney cancer, or bladder cancer. A change in urines smell can be a sign of an infection or a kidney stone; sweet-smelling urine may indicate diabetes. What is one of the most common ways to detect chemicals in urine? One of the most common ways to detect chemicals in urine is to use a dipstick, which is a stick coated with various chemical indicators. List several substances that one can test for in the urine using a dipstick. One can test for leukocytes, nitrite, urobilinogen, protein, blood, bilirubin, glucose, and others. How is a dipstick able to indicate differing amounts of certain substances in your urine? Dipsticks are able to indicate differing amounts of certain substances in your urine because they are coated with reagents that display different colors depending on the amounts of substances in urine. Morphine & Heroin: The Yin and Yang of Narcotics What is the source of morphine? Morphine is found in opium, the white sticky latex produced by poppy plants (Papver somniferum) How does opium differ from morphine? Opium is a mixture of different chemical compounds (sugars, proteins, fats, water and a specific class of compounds known as alkaloids) which includes morphine, an alkaloid. Heroin is chemically derived from morphine. Describe the chemical reaction that converts morphine to heroin. The conversion of morphine to heroin involves the addition of two acetyl groups (-CH3CO) to the morphine molecule where two OH groups are attached. Each of the two acetyl groups replaces the hydrogen (H) of an OH group, bonding to the main morphine structure. What is an endorphin? Endorphins are natural substances produced in the brain reducing the sensation of pain as well as inducing sleepiness and feelings of pleasure in the body. Morphine has the same effect. In what way is morphine like endorphins? Morphine has the same effect on the body as do endorphins. They both bind to the opioid receptors in the brain which causes a larger than normal release of the neurotransmitter dopamine. What happens to heroin, chemically, when it enters the brain? When heroin enters the brain, it is converted to morphine by the reverse chemical reaction by which morphine was converted to heroin. The two acetyl groups added to morphine to become heroin are removed in the reverse reaction. What is meant by drug tolerance? Drug tolerance occurs from repeated use of a particular drug, requiring higher doses of the same drug to be effective (same intensity of effect in the case of addictive drugs). Why is heroin usually injected directly into the bloodstream, instead of being taken by mouth as is done with morphine? Heroin is usually injected directly into the bloodstream because it is less soluble in waterbut more soluble in oils and fatsdue to the added acetyl groups. Once the heroin gets to the blood-brain barrier, it can sail right on through to the brain, while most water soluble molecules cantor at least go through the barrier much more slowly. What is dopamine? Dopamine is a neurotransmitter found in the brain. This chemical is responsible for producing feelings of euphoria as well as drowsiness. Why is morphine called a two-edged sword? Morphine is both blessing and a curse. It can be used very effectively to control pain. But if used for recreational purposes, it can lead to addiction which has all kinds of consequences, from medical issues to financial ones (buying more and more of the drug because of developing drug tolerance). So the drug has two sides to its usagea two edged sword. Opals: Playing with Color and Light Name the phenomenon that only opals, among all gemstones, display. The unique phenomenon that opals display is called play of color. How many silicon atoms surround each oxygen atom in the silica crystal? In the silica crystal, each oxygen atom is surrounded by two silicon atoms (see caption for Figure 1). How are sand and opal alike? How are they different? Sand and opals are alike in that they both are made of silicon dioxide, silica. Sand and opal are different in their structure: Sand is essentially weathered, broken-down quartz, in which the silica is arranged in an ordered crystalline 3-D pattern that extends in all directions, Opals structure consists of silica formed in multiple spheres instead of a single crystal, and water molecules surround the spheres. What special characteristic of the structure of precious opal gives it its characteristic play of color? The characteristic play of color exhibited by precious opal is due to the structure of the gem; the silica spheres are all the same size and theyre stacked in neat, precise layers. What is the name of the process that occurs when light is bent? The process that occurs when light is bent is called diffraction. Why do different colors appear inside an opal? Different colors appear inside an opal due to the difference in sizes of silica spheres in the opals structure. Larger spheres will bend longer wavelengths of light (the red end of the visible spectrum), while smaller spheres diffract shorter wavelengths (the blue end). At junctures inside the stone where sphere size is changing, reflected colors will change also. What two conditions are needed for opals to form? The two conditions needed for opal formation are silica-rich rock and short periods of rain followed by lengthy periods of drought. What is the formula for opal? Opals formula is SiO2*nH2O. What practical application has been made for synthetic polymer opals? A practical application for synthetic polymer opals is seen in the fashion industry. Fashion designers have used these polymer films in their fashion creations to reflect intense color and iridescence like real opals. How do these polymer films work? These polymer films also contain tiny spheres, just like real opals. But instead of silica, the polymer film spheres are made of a hard core of polystyrene surrounded by a softer shell of poly (ethyl acrylate). When heated, the spheres align themselves in a closely packed structure that diffracts light. The size and spacing of these spheres determines the colors of the film. ChemMatters Puzzle: An Element Fill-In Puzzle This puzzle will test your knowledge of the elements and their symbols, as well as a little bit of environmental (GREEN) chemistry. Below youll see 13 familiar terms from that subject area. They are NOT anagrams but do have a few letters missing. Your task is to fill in those spots. The missing letters will be SYMBOLS of an element (but are not case sensitive !). Well give you the NAMES of all the elements used today at the bottom. Be warned that elements up to atomic number 112 might be in play, so have a relatively new periodic table handy ! As a solving aid, any missing symbol will be used exactly once. As an example , if you are given E _ _ L _ G Y, the missing letters are Co and O, yielding E C O L O G Y, and Cobalt and Oxygen could be crossed off the list. Good luck with your element hunt. G L O _ _ L W _ _ M _ _ G C _ _ M _ _ E _ O _ _ R R E _ Y _ _ E E _ _ _ _ Y H _ _ _ I D E R _ _ _ O N H A _ _ _ _ T 9. _ _ L _ _ _ _ O N . _ _ I D _ _ I N . _ EA _ _ ER . R _ _O _ R _ _ S _ _A C _ I NG The element names in use (alphabetically). Do you know each ones symbol? Actinium Argon Astatine Barium Bismuth Bromine Carbon Cerium Chlorine Einsteinium Francium Indium Iodine Lanthanum Lithium Lutetium Neon Osmium Polonium Potasium Radium Roentgenium Sulfur Tantalum Thorium Titanium Tungsten Uranium Yttrium Answers to the ChemMatters Puzzle 1. GLOBAL WARMING Ba, Ar, In 2. CLIMATE Li, At 3 SOLAR S, La 4. RECYCLE C, Cl 5. ENERGY Ne, Rg 6. HYBRID Y, Br 7. EROSION Os ,I 8. HABITAT Bi, Ta 9. POLLUTION Po, Lu, Ti 10. ACID RAIN Ac, Ra 11. WEATHER W, Th 12. RESOURCES Es, U, Ce 13. FRACKING Fr, K Teachers might try challenging students to use this format (in which they insert element symbols into a half-dozen or so words that all deal with a given chemistry topic as a last-day- before- vacation exercise, or perhaps some extra credit. National Science Education Standards (NSES) Correlations National Science Education Content Standard Addressed As a result of activities in grades 9-12, all students should develop understanding Hot PeppersGlobal Climate ChangePeering Through UrineMorphine & HeroinOpalsPhysical Science Standard A: about scientific inquiry.(((Physical Science Standard B: of the structure and properties of matter.(((((Physical Science Standard B: of chemical reactions.(Earth Science Standard D: of geochemical cycles(Science and Technology Standard E: about science and technology.((((Science in Personal and Social Perspectives Standard F: of personal and community health.(((Science in Personal and Social Perspectives Standard F: of natural resources.((Science in Personal and Social Perspectives Standard F: of environmental quality.(Science in Personal and Social Perspectives Standard F: of natural and human-induced hazards.(((Science in Personal and Social Perspectives Standard F: of science and technology in local, national, and global challenges.(((History and Nature of Science Standard G: of science as a human endeavor.(History and Nature of Science Standard G: of the nature of scientific knowledge.(History and Nature of Science Standard G: of historical perspectives.(( Next-Generation Science Standards (NGSS) Correlations ArticleNGSSHot Peppers: Muy Caliente!HS-PS2-6. Communicate scientific and technical information about why the molecular-level structure is important in the functioning of designed materials. Crosscutting Concepts: Structure & Function Science and Engineering Practices: Obtaining, Evaluating, and Communicating Information Nature of Science: Scientific investigations use a variety of methods, tools, and techniques to revise and produce new knowledge.Global Climate Change: A Reality CheckHS-ESS2-2. Analyze geoscience data to make the claim that one change to Earths surface can create feedbacks that cause changes to other Earth systems. Crosscutting Concepts: Cause and Effect Stability and Change Influence of Engineering, Technology, and Science on Society and the Natural World Science and Engineering Practices: Developing and Using Models Analyzing and Interpreting Data Engaging in Argument from Evidence Nature of Science: Scientific knowledge is based on the assumption that natural laws operate today as they did in the past and will continue to do so in the future. Peering Through UrineHS-LS1-3. Plan and conduct an investigation to provide evidence that feedback mechanisms maintain homeostasis. Crosscutting Concepts: Structure and Function Stability and Change Science and Engineering Practices: Analyzing and interpreting data Nature of Science: Scientific investigations use diverse methods and do not always use the same set of procedures to obtain data. Morphine & Heroin: The Yin & Yang of NartoticsHS-ETS1-3. Evaluate a solution to a complex real-world problem based on prioritized criteria and trade-offs that account for a range of constraints, including cost, safety, reliability, and aesthetics as well as possible social, cultural, and environmental impacts. Crosscutting Concepts: Cause & Effect Stability & Change Science and Engineering Practices: Asking questions and defining problems Analyzing and interpreting data Nature of Science: Many decisions are not made using science alone, but rely on social and cultural contexts to resolve issues. Opals: Playing with Color and LightHS-PS1-1. Use the periodic table as a model to predict the relative properties of elements based on the patterns of electrons in the outermost energy level of atoms. HS-PS4-3. Evaluate the claims, evidence, and reasoning behind the idea that electromagnetic radiation can be described either by a wave model or a particle model, and that for some situations one model is more useful than the other. Crosscutting Concept: Patterns Cause and Effect Science and Engineering Practices: Engaging in Argument from Evidence Nature of Science: New technologies advance scientific knowledge.  Common Core State Standards Connections RST.9-10.1 Cite specific textual evidence to support analysis of science and technical texts, attending to the precise details of explanations or descriptions. RST 11-12.1 Cite specific textual evidence to support analysis of science and technical texts, attending to important distinctions the author makes and to any gaps or inconsistencies in the account. In addition, the teacher could assign writing to include the following Common Core State Standards: WHST.9-10.2 Develop the topic with well-chosen, relevant, and sufficient facts, extended definitions, concrete details, quotations, or other information and examples appropriate to the audiences knowledge of the topic. WHST.11-12.2 Develop the topic thoroughly by selecting the most significant and relevant facts, extended definitions, concrete details, quotations, or other information and examples appropriate to the audiences knowledge of the topic. Anticipation Guides Anticipation guides help engage students by activating prior knowledge and stimulating student interest before reading. If class time permits, discuss students responses to each statement before reading each article. As they read, students should look for evidence supporting or refuting their initial responses. Directions for all Anticipation Guides: Before reading, in the first column, write A or D indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. Hot Peppers: Muy Caliente! Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementThe chemical compound responsible for the heat found in the membrane of hot peppers is also found in smaller amounts in other spices.The Scoville heat index for peppers is based on what volunteer taste testers reported.If you ate a pepper containing 10 mg of capsaicin in 1 kg of pepper, you would have a long-lasting burning sensation on your tongue.If you accidentally eat a pepper that is too hot, water is the best drink to soothe the pain.Capsaicin is a polar molecule.Hot peppers actually increase the temperature in your mouth.Eating hot peppers is popular in warm climates because they make you feel cooler.Capsaicin is found in creams used to treat pain such as sore muscles.Chili peppers have very few vitamins. Global Climate Change: A Reality Check Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementThe Earths average surface temperature has increased by almost 1(C in the past century, and the sea level has risen by about 8 inches.There were about twice as many flood events in Southeast Asia in the first decade of the 21st Century than there were in the 1960s.Greenhouse gases include CO2, CH4, N2O, and H2O.The oceans would be frozen year-round if there were no greenhouse gases.N2O emissions are caused by synthetic fertilizer use.Ice-core samples indicate that the atmospheric concentration of CO2 is higher than at any time in the past 400,000 years.During the past century, a few regions have cooled.Most models predict that if we continue burning fossil fuels at the same rate, the concentration of CO2 in the atmosphere will triple.A tree can absorb one ton of CO2 over its lifetime.Alternative energy sources such as wind, solar, and geothermal have little effect on greenhouse gas emissions. Peering through Urine Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementMost people produce less than one liter of urine a day.If you are dehydrated, your urine is darker because there is less water to dissolve the chemicals, so their concentration is higher.Urine can be green, blue, orange, yellow, or wine-colored, due to different medications, vitamins, foods, or genetic conditions.Some medical conditions can be detected by simply observing urine.Dipsticks can measure pH, glucose, leukocytes, and concentration of urine.The pH of urine ranges from 7-9.Glucose is usually found in urine.Dipsticks can tell which antibiotics would be most effective in treating an infection.Scientists do not know why eating asparagus causes the urine of many people to smell. Morphine & Heroin: The Yin & Yang of Narcotics Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementMorphine is more addictive than heroin.Opium is a white sticky latex consisting of many chemicals.Morphine is an alkaloid derived from opium from poppy plants.Usually, morphine for medical use is administered in doses of 5-30 mg every three to four hours.Codeine is safer than morphine.Morphine dulls the senses, relieving pain and producing feelings of pleasure.Morphine is produced from heroin.Heroin is more soluble in water than morphine is.The number of heroin users in the United States is increasing, and almost one-quarter of them become addicted. Opals: Natures Colorful Gemstones Directions: Before reading, in the first column, write A or D, indicating your agreement or disagreement with each statement. As you read, compare your opinions with information from the article. In the space under each statement, cite information from the article that supports or refutes your original ideas. MeTextStatementOpals are made of the same compound as sand, silicon dioxide.Opals have a crystalline structure.The play of color in opals is a result of light diffraction.The flash of different colors in opals depends on the viewing angle.Opals are found deep (more than 50 meters) under ground.Opals are found in wet climates.Opals contain water, so they are not as hard as other gemstones.Synthetic opals often have more dramatic color than those found in nature.Stretchable polymer opal films used in clothing are made of the same chemical as opals.Opals, quartz, and amethyst all contain silica. Reading Strategies These graphic organizers are provided to help students locate and analyze information from the articles. Student understanding will be enhanced when they explore and evaluate the information themselves, with input from the teacher if students are struggling. Encourage students to use their own words and avoid copying entire sentences from the articles. The use of bullets helps them do this. If you use these reading strategies to evaluate student performance, you may want to develop a grading rubric such as the one below. ScoreDescriptionEvidence4ExcellentComplete; details provided; demonstrates deep understanding.3GoodComplete; few details provided; demonstrates some understanding.2FairIncomplete; few details provided; some misconceptions evident.1PoorVery incomplete; no details provided; many misconceptions evident.0Not acceptableSo incomplete that no judgment can be made about student understanding Teaching Strategies: Links to Common Core State Standards for writing: Ask students to revise one of the articles in this issue to explain the information to a person who has not taken chemistry. Students should provide evidence from the article or other references to support their position. Vocabulary that is reinforced in this issue: Nanoparticles. Structural formulas. (You may want to have model kits available to help students visualize the structures.) To help students engage with the text, ask students what questions they still have about the articles. The article about climate change, in particular, may spark questions and even debate among students. Hot Peppers: Muy Caliente! Directions: As you read the article, complete the graphic organizer below describing what you learned about hot peppers. 3Your friends want to enjoy some really hot, spicy food. Write three new things you learned about hot peppers from reading this article that you would like to share with your friends. 1. 2. 3.2Share two things you learned about chemistry from the reading the article. 1. 2.1Did this article change your views about eating hot spicy food? Explain in one sentence.Contact!Describe a personal experience about eating hot spicy food that connects to something you read in the articlesomething that your personal experience validates. Global Climate Change: A Reality Check Directions: As you read the article, complete the graphic organizer below to outline the evidence for climate change. Beginning Ideas: Questions you have about climate changeTestsTime frame ObservationsTemperatureSea LevelTropical StormsIce-Core SamplesOtherPredictions: What do the models predict for the future? Possible solutions: Include possible drawbacks.Reflection: How have your ideas about climate change changed?  Peering through Urine Directions: As you read, use the chart below to describe how urine can help doctors diagnose medical problems. TestPossible diagnosisDark colorCloudinessRed or brown colorChange in smellpHSugarLeukocytesNitrites Morphine & Heroin: The Yin & Yang of Narcotics Directions: As you read the article, use your own words to complete the graphic organizer below comparing morphine and heroin. At the bottom, list properties they have in common. MorphineHeroinAddictive propertiesChemical structureMedical useEffect in the brainSolubility in waterPotencyLegalitySimilarities Opals: Natures Colorful Gemstones Directions: As you read, use your own words to describe opals using the graphic organizer below. DescriptionSignificanceChemical ElementsStructureLarger spheresSmaller spheresLight DiffractionLight InterferenceWhere opals are foundHow to care for opalsSynthetic opalsPolymer opals Hot Peppers: Muy Caliente! Background Information (teacher information) More on chili peppers The picture the Rohrig article paints of chili peppers (also written as chile peppers, chilli peppers, chilies, and other variations) tends toward the painful side. His initial description of the effects of eating a Moruga Scorpion pepper might lead one to wonderwhy would anyone willingly subject themselves to such an experience? There are some benefits associated with chili peppers. The article states that theyre an excellent source of vitamins C, A, and E, as well as folic acid and potassium. Chili peppers and the chemical compounds they contain may be able to help with weight loss and can also help to mask the pain of arthritis, shingles, and sore muscles. Aztec Indians used them as a treatment for toothache; the Mayans used them for asthma, coughs, and sore throats. ( HYPERLINK "http://www.rsc.org/Education/EiC/issues/2006May/SpicingupChemistry.asp" http://www.rsc.org/Education/EiC/issues/2006May/SpicingupChemistry.asp) Ed Currie, developer of the Carolina Reaper, another chili pepper contending for the worlds hottest crown, eats chili peppers daily in the belief that they help keep cancer from returning to his body. (HYPERLINK "http://www.cbsnews.com/8301-505269_162-57589329/"http://www.cbsnews.com/8301-505269_162-57589329/) He even donates a large portion of his annual chili pepper crop to cancer researchers, who are investigating the ability of capsaicin (the main compound that gives chili peppers their hotness) to kill cancer cells. The nastiness of the chili peppers themselves can also be turned into a useful tool. The Mayans burned chiles to create a stinging smoke screen, and threw gourds filled with pepper extract in battle. Nowadays, capsaicin is the active ingredient in pepper sprays, used to ward off attacking muggers, dogs, and bears. ( HYPERLINK "http://antoine.frostburg.edu/chem/senese/101/features/capsaicin.shtml" http://antoine.frostburg.edu/chem/senese/101/features/capsaicin.shtml) Eating peppers as a part of food dishes is widespread around the world. The article An Overview about Versatile Molecule Capsaicin summarizes: Chili peppers are mainly consumed as food additives in many regions of the globe because of their unique pungency, aroma, and color. Indeed, a quarter of the worlds population consumes hot pepper in some form daily. (Arora, R.; Gill, N.S.; Chauhan, G.; Rana, A.C. An Overview about Versatile Molecule Capsaicin. Int. J. Pharmaceut. Sci. Drug Res. 2011, 3 (4), p 280; see  HYPERLINK "http://www.ijpsdr.com/pdf/vol3-issue4/2.pdf" http://www.ijpsdr.com/pdf/vol3-issue4/2.pdf) In addition to this consumption of peppers that have ordinary levels of heat, many people are part of a trend of eating hotter and hotter peppers. The Moruga Scorpion and others like it are sometimes termed superhots. Many videos online document peoples experiences of eating various peppers, sometimes to extreme results, such as vomiting on camera. Some vendors even sell capsaicin extract, so users can add it directly to any food or hot sauce they dont deem hot enough. Part of the interest in eating the peppers may be the appeal of the experience to thrill-seekers. The body responds to the pain and stress of eating a pepper with an endorphin rush, giving one a mix of pain with the pleasure. The author of The New York Times article A Perk of Our Evolution: Pleasure in Pain of Chilies quotes Dr. Paul Rozin, who has a Ph.D. in both biology and psychology: he has evidence for what he calls benign masochism. For example, he tested chili eaters by gradually increasing the pain, or, as the pros call it, the pungency, of the food, right up to the point at which the subjects said they just could not go further. When asked after the test what level of heat they liked the best, they chose the highest level they could stand, just below the level of unbearable pain. No one knows for sure why humans would find pleasure in pain, but Dr. Rozin suggests that theres a thrill, similar to the fun of riding a roller coaster. Humans and only humans get to enjoy events that are innately negative, that produce emotions or feelings that we are programmed to avoid when we come to realize that they are actually not threats, he said. Mind over body. My body thinks Im in trouble, but I know Im not. (Gorman, J. A Perk of Our Evolution: Pleasure in Pain of Chilies. The New York Times, Sep. 20, 2010; see  HYPERLINK "http://www.nytimes.com/2010/09/21/science/21peppers.html?_r=0" http://www.nytimes.com/2010/09/21/science/21peppers.html?_r=0) The past ChemMatters article Pepper Power discusses the origin of chili peppers: Capsaicin is found in plants of the genus Capsicumcommonly known as chili peppersand is responsible for their burning hot taste. Hot peppers originated in South America, where they have been cultivated since 5500 B.C., and were introduced to Europe and Asia after discovery by Columbus. Though the peppers were not well received by Europeans, they quickly became popular in India and China. (Williams, C. Pepper Power. ChemMatters 1995, 13 (2), p 11) The pepper in the name comes from Columbuss mistaken idea that the capsaicin-containing peppers he found were related to black pepper plants, which get their spiciness from the compound piperine rather than capsaicin and its related compounds. What we consider the hotness or heat of a chili pepper is referred to as pungency. The majority of the pungency in a chili pepper comes from capsaicin, but there are other related compounds that also contribute. This group of compounds is known as the capsaicinoids. There are over 22 known capsaicinoids. ( HYPERLINK "http://aces.nmsu.edu/pubs/_h/H237/" http://aces.nmsu.edu/pubs/_h/H237/) Besides capsaicin, some of the compounds within this group are 6,7-dihydrocapsaicin, nordihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin. Capsaicin and dihydrocapsaicin, between the two of them, typically contribute ~8090% of the pungency of chili peppers. In the figure below, one can see that each has a long hydrocarbon tail, with small variations within the tail. These small variations can have an impact on what we experience when eating a pepper: Several capsaicin-like compounds found in chiles have slight structural variations in the hydrocarbon tail, which changes their ability to bind to the receptors and their ability to penetrate layers of receptors on the tongue, mouth, and throat. That may explain why some chiles burn in the mouth, while others burn deep in the throat. ( HYPERLINK "http://antoine.frostburg.edu/chem/senese/101/features/capsaicin.shtml" http://antoine.frostburg.edu/chem/senese/101/features/capsaicin.shtml) Chili pepper connoisseurs have different terms to describe the varying types of pungency that different peppers can produce: sharp, piercing, stinging, biting, burning, or penetrating. ( HYPERLINK "http://aces.nmsu.edu/pubs/_h/H237/" http://aces.nmsu.edu/pubs/_h/H237/)  (from  HYPERLINK "http://www.mdidea.com/products/new/new00504.html" http://www.mdidea.com/products/new/new00504.html) The Smithsonian.com blog Food & Think describes a further cataloging of how one experiences the pungency of a pepper, discussing the ideas of Dr. Paul Bosland, a professor of horticulture and the author of several books on chili peppers: Bosland and his colleagues have broken the heat profile of chile peppers into five distinctly different characteristics. 1) how hot it is, 2) how fast the heat comes on, 3) whether it linger or dissipates quickly, 4) where you sense the heat on the tip of tongue, at the back of throat, etc., and 5) whether the heat registers as flat or sharp. This last characteristic is fascinating for what it says about cultural chile pepper preferences (say that five times fast). Apparently those raised in Asian cultures where chile heat has been considered one of the six core tastes for thousands of years prefer sharp heat that feels like pinpricks but dissipates quickly. Most Americans, on the other hand, like a flat, sustained heat that feels almost like its been painted on with a brush. ( HYPERLINK "http://blogs.smithsonianmag.com/food/2013/01/how-hot-is-that-pepper-unpacking-the-scoville-scale/" http://blogs.smithsonianmag.com/food/2013/01/how-hot-is-that-pepper-unpacking-the-scoville-scale/) So, a chili peppers heat comes from capsaicin and related compounds, but how does a peppers particular combination come about in the first place? The heat level in chile peppers is the result of two factors: the plants genetics and the interaction of the plant with the environment. The genetic control of heat allows plant breeders to produce a chile pepper plant with a certain relative heat level. For example, the cultivar NuMex Joe E. Parker was genetically selected to produce fruit of medium heat. However, environmental factors such as temperature and water influence the heat level. A mild chile pepper cultivar bred for low levels of heat will become hotter when exposed to any type of stress in the field. Conversely, a relatively hot cultivar given optimal environmental conditions will become only moderately hot. A chile pepper plant that genetically produces low-heat fruit will not produce hot chile peppers even when grown in a stressed environment. To produce chile peppers of a predictable heat, both cultivar selection and optimum stress-free growing conditions are important. (HYPERLINK "http://aces.nmsu.edu/pubs/_h/H237/"http://aces.nmsu.edu/pubs/_h/H237/) Within the pepper itself, the Rohrig article states that capsaicin is primarily found in the membrane that holds the seeds. Further information about the specific locations is available in the article An Overview about Versatile Molecule Capsaicin: Recent studies indicate that capsaicin is mostly located in vesicles or vacuole like sub-cellular organelles of epidermal cells of placenta in the pod. The highest concentrations of capsaicin are found in the ovary and in the lower flesh (tip) and the lowest content of capsaicin can be found in the seeds. The gland on the placenta of the fruit produces capsaicinoids. The seeds are not the source of pungency but they occasionally absorb capsaicin because they are in close proximity to the placenta. No other plant part produces capsaicinoids. The majority, about 89%, of the capsaicin is associated with the placental partition of the fruit and nearly 56% in the pericarp and the seed. Composition of capsaicin may vary among different varieties of the same species and with fruit of a single variety. The pungency is influenced with the weather conditions such as heat wave and it increases with the growth of the maturity of the fruit. (Arora, R.; Gill, N.S.; Chauhan, G.; Rana, A.C. An Overview about Versatile Molecule Capsaicin. Int. J. Pharmaceut. Sci. Drug Res. 2011, 3 (4), pp 280281; see  HYPERLINK "http://www.ijpsdr.com/pdf/vol3-issue4/2.pdf" http://www.ijpsdr.com/pdf/vol3-issue4/2.pdf)  (from Williams, C. Pepper Power. ChemMatters 1995, 13 (2), p 10) More on capsaicin The division of this More on capsaicin section from the More on chili peppers section above and the More on the Scoville heat scale section below is somewhat arbitrary. For example, one cannot even discuss the peppers genus name of Capsicum without at least mentioning the related name capsaicin. The Scoville heat scale compares items against the pungency of pure capsaicin. All three sections have a common thread that runs through themthe chemical compound that typically provides the majority of the pungency of a chili pepper. Methods to more objectively detect and determine the amount of capsaicin present in a sample have come a long way since the development of the Scoville Organoleptic Test, which depends on human taste testers. The journal article Chemical and Pharmacological Aspects of Capsaicin states: Growing interest in capsaicin has led to its characterization with methods such as spectrophotometry UV-VIS and chromatography. These have been modified over time to develop more sensitive, faster capsaicin characterization techniques. (de Lourdes Reyes-Escogido, M.; Gonzalez-Mondragon, E.G.; Vazquez-Tzompantzi, E. Chemical and Pharmacological Aspects of Capsaicin. Molecules 2011, 16 (2), p 1259; see  HYPERLINK "http://www.mdpi.com/1420-3049/16/2/1253" http://www.mdpi.com/1420-3049/16/2/1253) The article goes on to discuss an extensive listing of additional techniques, including thin-layer chromatography, multi-band thin-layer chromatography, high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), liquid chromatography-tandem mass spectrometry (LC-MS-MS), liquid chromatography quadrupole ion trap mass spectrometry (LC-ESI/MS/MS), gas chromatography (GC), and solid phase microextraction-gas chromatography-mass spectrometry. HPLC is the method mentioned quite often in connection with capsaicin. A Royal Society of Chemistry student handout includes an explanation of the technique along with a diagram of the HPLC apparatus: This technique is essentially about separating mixtures. It works on the same principle as paper chromatography in that it involves a solvent moving through a solid and carrying with it the components of a mixture, which travel at different rates. In HPLC, the solid phase is a powder enclosed in a metal tube called a column and the solvent is forced through the column by a high pressure pump, so HPLC is sometimes said to refer to High Pressure Liquid Chromatography. A sample of the mixture to be separated is injected into the solvent just before it enters the column. The better a component forms intermolecular bonds with the liquid, the faster it moves through the column and the sooner it emerges from the other end. The time a component spends on the column is called the retention time. A particular substance will always have the same retention time provided that the solid phase and the solvent are kept the same so the retention time can be used to identify components of a mixture. (It is possible that two unrelated substances may have the same retention time so we need to have some idea of the likely components of the mixture to use the retention time to identify them.) A detector times when the different components emerge from the column and measures how much there is of each. A computer is then used to plot a graph (called a chromatogram). The height of each peak (strictly the area below it) represents the amount of that component present in the mixture while the position on the horizontal axis shows the retention time, which can be used to identify the substance causing the peak. When using HPLC to measure the hotness of a chilli, the analyst uses the chromatogram to measure the amounts of different capsaicinoids present. The greater the amount of these, the hotter the chili. She or he must also allow for the different hotnesses of the different capsaicinoid compoundshomocapsaicin is only about half as hot as capsaicin, for example. The method must be calibrated by a human taster to convert the measured amounts of capsaicinoid compounds into Scoville units as no machine can actually measure the sensation of taste. To carry out the test, a chilli is dried, weighed, ground and a solvent is added in which the capsaicinoid compounds will dissolve. A known volume of this solution is injected onto the HPLC column. ( HYPERLINK "http://www.rsc.org/education/teachers/resources/kitchenchemistry/docs/SS16c.pdf" http://www.rsc.org/education/teachers/resources/kitchenchemistry/docs/SS16c.pdf)  Sample HPLC chromatograms showing capsaicin and dihydrocapsaicin peaks are shown in a short online handout from the instrumentation company Shimadzu. One of the figures is shown below.  (from  HYPERLINK "http://www2.shimadzu.com/applications/lc/L335.pdf" http://www2.shimadzu.com/applications/lc/L335.pdf) The uses of capsaicin are varied and extend well beyond the idea of adding chili peppers to ones food to add a bit of spice. One use is within the chili pepper itself. Capsaicin may be able to help protect the chili pepper from a fungal infection. The New York Times article A Perk of Our Evolution: Pleasure in Pain of Chilies says, A recent study suggested that capsaicin is an effective defense against a fungus that attacks chili seeds. In fact, experiments have shown that the same species of wild chili plant produces a lot of capsaicin in an environment where the fungus is likely to grow, and very little in drier areas where the fungus is not a danger. (Gorman, J. A Perk of Our Evolution: Pleasure in Pain of Chilies. The New York Times, Sep. 20, 2010; see  HYPERLINK "http://www.nytimes.com/2010/09/21/science/21peppers.html?_r=0" http://www.nytimes.com/2010/09/21/science/21peppers.html?_r=0) Other products use compounds from the pepper. Pepper spray is perhaps the best-known product that uses capsaicin. The spray is also sometimes called OC spray, since it contains oleoresin capsicum. The 1995 ChemMatters article Pepper Power describes it: Twenty kilograms of dried, whole peppers, when treated with solvents, yields about one kilogram of crude extract known as oleoresin capsicum (OC). OC is a mixture of essential oils, waxes, terpenes, resin acids, and several capsaicinoid compounds, the most potent of which is capsaicin. (Williams, C. Pepper Power. ChemMatters 1995, 13 (2), p 11) Other products that function to keep undesired organisms away using capsaicin are those such as Hot Pepper Wax, a product that: is a mixture of capsaicin and wax used to protect apples, grapes, and various vegetables. Walter Wilson, president of the company says, If insects are coated by the wax, 70% of them are killed because their nervous systems are overstimulated. It causes them to defecate endlessly until they die. Later, other insects avoid the treated plants because the pepper aroma is irritating. Most pesticides are toxic, which means that their use is tightly regulated; they cannot be applied just before the food is picked and sent to market. In contrast, the government has approved Hot Pepper Wax for application right up to the time of harvest. (Williams, C. Pepper Power. ChemMatters 1995, 13 (2), p 12) Paints that contain OC may also be able to prevent barnacles from collecting on the bottoms of ships. Potential medical uses of capsaicin relate to anticancer activity, weight management, and cardiovascular conditions. The Rohrig article describes capsaicins use as a pain reliever as it affects the sensitivity of the skin to pain. It is this property that has caused capsaicin-containing products to be banned from use in equestrian sports. In the 2008 Summer Olympics, four horses were actually disqualified from the show jumping competition for testing positive for capsaicin. One of the riders claimed he was using it to treat his horses back pain. However, its use had been banned because some riders took advantage of the period of increased sensitivity of the skin to force horses to lift their legs higher over the jumps to avoid the increased pain that would come from hitting the sensitized skin. ( HYPERLINK "http://www.rsc.org/chemistryworld/podcast/CIIEcompounds/transcripts/capsaicin.asp" http://www.rsc.org/chemistryworld/podcast/CIIEcompounds/transcripts/capsaicin.asp) The 1995 ChemMatters article Pepper Power offers an extensive explanation of how capsaicin can both cause and relieve pain. The answer lies in the way nerve cells, or neurons, communicate. Neurons carry messages to and from the brain in the form of electrochemical impulses. Each neuron has a cell body from which protrude dendrites that receive information and an axon that carries the impulses to the other end. At the end of the axon is a gap, or synapse, that separates the cell from the next neuron. The impulse must be transmitted to the next neuron by neurotransmitters (chemicals released by the first neuron), which allow it to cross the synapse and bind to the dendrites of the next neuron. Other types of chemicals, called neuromodulators, also may be released. These do not directly cause transmission of a nerve impulse but affect how easily an impulse can be sent. Neuropeptides chains of amino acids used by nerves sometimes act as neuromodulators. Capsaicin affects the primary sensory nociceptive neurons. These nerves are found in the skin and tongue and originate the pain messages that flow first to the spinal nerves, then to the brain. Primary nociceptive neurons use several neurotransmitters, including the amino acid glutamate and the neuropeptide substance P. Substance P named for pain also acts as a neuromodulator and makes nerves more sensitive to pain stimuli. When a neuron is at rest, its membrane is a selective barrier to ions. Potassium ions are much more concentrated inside the cell, whereas sodium and calcium ions are more concentrated outside. The membrane is riddled with minute holes or ion channels that open and close to control the passage of the ions. Near the outer entrance of the ion channel is a receptor a protein designed to fit only certain molecules. When a molecule fits, the receptor and the ion channel open and let certain ions, such as Na+ and Ca2+, rush in. Once a large number of ions enter the cell, adjacent ion channels change shape, letting in more positive ions, which open neighboring ion channels and so on. This chain reaction of ion flow is the nerve impulse. Eventually, the impulse reaches the end of the axon and releases neurotransmitters that trigger an impulse in the spinal nerves, sending a message to the brain Ouch! After the neurotransmitter leaves the receptor, the channel closes and the cell pumps the ions back to their original locations. So what is the effect of capsaicin? When the capsaicin reaches a neuron it binds to a receptor that opens the ion channel for Na+ and Ca2+. This starts the impulse that releases substance P. As a neuromodulator, substance P hypersensitizes neighboring neurons makes them more easily excited. Now, less stimulation is required to trigger a sensation of pain. Whats more, substance P induces inflammation, which makes the area even more painful. Instead of quickly leaving the receptor, the capsaicin molecule sticks tenaciously, holding the channel open. Na+ and Ca2+ keep entering the cell, triggering impulses and causing intense pain. This explains the intense pain felt by James [earlier described in article as being sprayed with pepper spray] but doesnt explain capsaicins ability to reduce the pain caused by Sharons diabetes. In the clinic, Sharon was given a local anesthetic before capsaicin was applied to her skin. The capsaicin caused massive amounts of substance P to be released. Because the local anesthetic blocked all impulses, she didnt feel the surge of pain that can stop a criminal. The capsaicin locks open the ion channels until according to one hypothesis the cell depletes its supply of neurotransmitter. When the anesthetic wears off, any stimulation of the neuron is less effective because there is not as much substance P left to release. The patient experiences less pain. Another hypothesis emerged when it was discovered that capsaicin can kill very slender nerve fibers. If the capsaicin locks open the ion channels long enough, the pain-sensing fibers may vital lose [sic] fluids and die. However it works, the therapy gradually becomes more effective. After a few weeks of treatment in the clinic, patients can skip the local anesthetic and apply the ointment at home. This is not a cure. Many patients must apply the ointment four times a day, and the pain returns within two weeks if the treatment is stopped. Capsaicin has been used for pain reduction since the early 1980s and, in addition to its use in diabetes treatment, it has been prescribed for osteoarthritis, rheumatoid arthritis, postmastectomy pain, and peripheral neuropathies. According to [Dr. Miroslav] Backonja, capsaicin treatment is limited to peripheral conditions in which the painful tissues lie close to the surface and the skin is abnormally permeable because of irritation and damage. It is most effective when used in conjunction with other pain treatments. (Williams, C. Pepper Power. ChemMatters 1995, 13 (2), pp 1213) Because of these various uses and benefits, researchers are interested in increasing the amount of capsaicin produced by chili peppers or finding other effective ways of obtaining capsaicin. There is also a hunt for similar compounds that can serve the same purpose as capsaicin, but without the unpleasant side effects connected with its overwhelming pungency. More on the Scoville heat scale Need to make a judgment on how hot a chili pepper is? Making such a decision could be based on something as simple as just taking a bite of the pepper. For example, an article in The Washington Post describes the pepper rating system employed by the company Johnnys Selected Seeds: I asked Johnnys founder Rob Johnston to explain the rating system. His response: Steve Bellavia, our trials manager, takes a bite. Janicka Eckert, our breeder, does, too. They consult. Bellavia is a passionate pepper-lover whose palate is one to trust. So is your own, because taste is highly subjective. (Damrosch, B. Growing Peppers, Hot and Hotter. The Washington Post, May 9, 2012; see  HYPERLINK "http://articles.washingtonpost.com/2012-05-09/lifestyle/35457181_1_heat-units-pepper-fruits-pepper-lover" http://articles.washingtonpost.com/2012-05-09/lifestyle/35457181_1_heat-units-pepper-fruits-pepper-lover) The company uses these results to rate the pepper seeds it sells as producing peppers that rank from one pepper (mild) to five peppers (very hot, also described as mouth-blistering heat). Looking for a test thats a little more scientific? Rohrigs article describes the Scoville heat scale. The design of the test still depends on the subjectiveness of human testers, but adds a quantitative aspect. Chili pepper extract is diluted in increasing amounts of sugar water. Testers continue to taste the samples until they can no longer personally detect the pungency of the chili in the solution theyre tasting. That amount of dilution is then translated into Scoville Heat Units. For an even more quantitative test, HPLC (described in the Background Information section More on capsaicin above) can be used to analyze an extract of a chili pepper to determine the amounts of specific chemicals responsible for the pungency of the pepper. The methods are compared in the New Mexico State University horticulture publication Measuring Chile Pepper Heat: This procedure [Scoville test] can be appropriate in many circumstances because it is more accurate than the taste test (bite the chile) technique. The Scoville Organoleptic Test is also less expensive than more advanced laboratory techniques, but it has limitations. Measuring heat with this technique is still subjective and depends on the tasters palate and sensitivity to the chemicals that are responsible for heat. In addition, there are serious limits on how many samples a taster can handle within a reasonable time. The most accurate method for measuring heat in chile peppers is high-performance liquid chromatography (HPLC). This method is more costly than the Scoville test, but it gives an objective heat analysis. Not only does this method measure the total heat present, it also allows the amounts of the individual capsaicinoids to be determined. In addition, many samples may be analyzed within a short period. The NMSU Chile Breeding and Genetics Program has analyzed more than 5,000 samples using this method and has found it to be reliable and consistent. As the demand for chile peppers increases, the heat level of the crop is more important, and an accurate and precise measurement of heat is necessary. Several testing laboratories perform the organoleptic and/or HPLC methods. The American Spice Trade Association (ASTA) publishes the procedure accepted by the spice industry. (Bosland, P.W.; Walker, S.J. Measuring Chile Pepper Heat. Guide H-237; see  HYPERLINK "http://aces.nmsu.edu/pubs/_h/H237/" http://aces.nmsu.edu/pubs/_h/H237/) History of Wilbur Scoville Wilbur Scoville presented a paper describing his Scoville Organoleptic Test in 1912. However, his connection to chili peppers extends well before that. Scoville had published the book The Art of Compounding in 1895. The book, along with a quote it contained related to chili peppers, is described by Dave DeWitt in a biographical essay on Scoville: After just three years on the college faculty, when he was just thirty years old in 1895, his best-known work, The Art of Compounding, was published. The book was used as a standard pharmacological reference up until the 1960s. The subtitle of the book, A Text Book for Students and a Reference Book for Pharmacists at the Prescription Counter, gives us a clue as to why the book was so popularthere were two markets for it. I found a copy of this book in Google Books, and here are two notable quotes that I discovered. Scoville was one of the first, if not the first person to suggest in print that milk is an antidote for the heat of chiles. Milk, as ordinarily obtained, he wrote, is seldom used except as a diluent [diluting agent]. In this capacity it serves well for covering the taste of sharp or acrid bodies as tinctures of capsicum, ginger, etc., and for many salts, chloral, etc. ( HYPERLINK "http://www.fiery-foods.com/article-archives/85-chile-history/3171-wilbur-scoville-and-the-organoleptic-test-centennial" http://www.fiery-foods.com/article-archives/85-chile-history/3171-wilbur-scoville-and-the-organoleptic-test-centennial) (The second notable quote mentioned in the excerpt above goes on to discuss the issue of addiction rather than chili peppers.) Over ten years after the publication of this book, Scoville was hired by the pharmaceutical company Parke, Davis & Company. It was his work there on the companys muscle salve product Heet that would lead to the development of the Scoville Organoleptic Test. The term organoleptic means involving the use of the sense organs. Scovilles work is described: Heet was made with chile peppers and the problem was standardizing the type and the amount of chiles that needed to be added to the other ingredients of Heet to standardize the formulation and avoid burning the skin of the person using it. Scoville was assigned to solve this problem, which took a few years due to his other duties. In the earliest reference to his work on chiles, the American Journal of Pharmacy noted in 1911: Wilbur L. Scoville presented a Note on Capsicum, showing the great variation in the strength of capsicum, and suggesting the possibility of the pungency of this drug being used as a simple test for quality. This paper elicited some discussion in the course of which it was pointed out that the physiological test for capsicum was infinitely more delicate and more reliable than the similar test that has been proposed for use in connection with aconite. At the American Pharmaceutical Association annual meeting in Denver in 1912, Scoville presented a paper on his solution to the Heet problem: the Scoville Organoleptic Test. Albert Brown Lyons, writing in Practical Standardization by Chemical Assay of Organic Drugs and Galenicals (1920), explains. It is quite possible to form a reasonably exact judgment of the strength of a sample of the drug [capsaicin] by the simple expedient of testing its pungency. W. L. Scoville proposes the following practical method. Macerate 0.1 gm. of ground capsicum overnight in 100 mils of alcohol; shake well and filter. Add this tincture to sweetened water (10% sugar) in such proportion that a distinct but weak pungency is perceptible to the tongue or throat. According to Scoville official capsicum will respond to this test in a dilution of 1 : 50,000. He found the Mombassa chilles to test from 1 : 50,000 to 1 : 100,000; Zanzibar chillies, 1 : 40,000 to 1 : 45,000; Japan chillies 1 : 20,000 to 1 : 30,000. Nelson found that a single drop of a solution of capsaicin in alcohol 1 : 1,000,000, applied to the tip of the tongue produced a distinct impression of warmth. ( HYPERLINK "http://www.fiery-foods.com/article-archives/85-chile-history/3171-wilbur-scoville-and-the-organoleptic-test-centennial?start=1" http://www.fiery-foods.com/article-archives/85-chile-history/3171-wilbur-scoville-and-the-organoleptic-test-centennial?start=1) Connections to Chemistry Concepts (for correlation to course curriculum) ConcentrationThe article discusses concentration units of parts per million in connection with determining the concentration of capsaicin in peppers. Instrumental AnalysisMore subjective measurements of how hot a chili pepper is (e.g., eating a pepper directly, Scoville-type testing where pepper extracts are diluted in sugar solutions and tasted) can be contrasted with more objective instrumental analysis (e.g., high performance liquid chromatography) that can be used to determine the amount of capsaicin and related compounds in a chili pepper. PolarityThe structure of capsaicin, including its long nonpolar hydrocarbon tail, can be compared and contrasted with the structures of compounds present in foods and drinks that one might use to combat the heat/pain of eating a chili pepper. The article discusses water, milk, ice cream, and bread. BiochemistryThe article touches on biochemistry concepts such as the lock-and-key fit of a capsaicin molecule in specific pain receptors and the actions set off in the body by that fit, including the release of neurotransmitters. Pharmaceutical ChemistryThe article describes the use of capsaicin to treat the pain of conditions such as arthritis, shingles, and sore muscles. Possible Student Misconceptions (to aid teacher in addressing misconceptions) A chili peppers seeds are the part that contains the most capsaicin. The highest concentration of capsaicin in a chili pepper is typically found in the placenta, or membrane, that holds the seeds, rather than the seeds themselves. The seeds do not produce capsaicin, but can absorb some from the placenta. Chili peppers are related to black pepper. While both chili peppers and black pepper share a common name, are used as spices, and have a pungency, or spiciness/hotness associated with them, they are not related. The pungency in chili peppers is due to the compound capsaicin, while black peppers pungency is caused by piperine. When you eat a hot pepper, your mouth will show a rise in temperature. If you placed a thermometer in your mouth after eating a hot pepper, it will not show a rise in temperature. The pepper triggers pain receptors that send a signal to the brain that is interpreted as heat. Anticipating Student Questions (answers to questions students might ask in class) Can eating chili peppers permanently damage your taste buds? The pungency of chili peppers does not act on taste buds; rather, it interacts with pain receptors in your mouth and throat. Eating chili peppers does not permanently damage your taste buds or the pain receptors. However, while still experiencing the pungency, you may feel that your sense of taste is somewhat dulled. Whats an unusual food product that contains capsaicin? Aside from the usual hot sauce products, students might be interested to know that the candy Atomic Fireballs contains capsaicin, which provides the burning sensation while eating the candy. The candy has a Scoville heat rating of 3,500. ( HYPERLINK "http://www.mnn.com/green-tech/research-innovations/stories/the-science-behind-some-of-your-favorite-candy" http://www.mnn.com/green-tech/research-innovations/stories/the-science-behind-some-of-your-favorite-candy) In-class Activities (lesson ideas, including labs & demonstrations) A titration experiment to quantitatively determine the amount of vitamin C present in green peppers was published in the April 1995 ChemMatters Classroom Guide. It uses an oxidationreduction titration with iodine. First, a solution made with a 100-mg vitamin C tablet is titrated. Then, an extract of green pepper is prepared and titrated. (Darrow, F.W. ChemMatters Classroom Guide. April 1995, p 3) The April 1995 ChemMatters Classroom Guide also describes a qualitative demonstration to illustrate the vitamin C content of green peppers: ( this demonstration is based on one described in Carl H. Snyders The Extraordinary Chemistry of Ordinary Things; 2nd edition, Wiley, 1994, ISBN 0471310425, p. 492). First show the reducing power of vitamin C by putting a vitamin C tablet in about 100 mL of water, swirling for a minute or so to dissolve some of the tablet, and then decanting the water into a Petri dish or small beaker on an overhead. Carefully add a few drops of tincture of iodine and observe the iodine color disappear. If you add a few drops of ordinary household bleach the iodine color will briefly reappear as the iodide ion is oxidized back to iodine by the bleach. Further oxidations cause the iodine color to disappear, so you have to be careful or you will miss it. Now take a few pieces of green pepper and thoroughly mash them in a little water to extract their vitamin C (a mortar would be useful, but is not necessary). Decant the solution as with the vitamin C tablet and perform the same qualitative test. The same demonstration can be done with paprika or chili powderuse a lot of powder and then filter to remove the powder residue. (Darrow, F.W. ChemMatters Classroom Guide. April 1995, p 2) The April 1995 ChemMatters Classroom Guide describes an additional demonstration using peppers that shows their catalytic activity: Capsicum peppers are members of the Solanacea (or nightshade) family as are tomatoes and potatoes. Like the other Solanacea plants they contain significant amounts of peroxidase, an enzyme that catalyzes the rapid decomposition of peroxides. You can demonstrate this catalytic activity by putting a little 3% hydrogen peroxide in a Petri dish on the overhead and then dropping in a few small pieces of green pepper. In a few moments rapid evolution of oxygen will be seen as bubbles and foam around the pepper pieces (the same thing happens with small pieces of potato). (Darrow, F.W. ChemMatters Classroom Guide. April 1995, p 2) A four-page Harvest of the Month document for California schools has information about sweet and hot peppers and offers various activities such as a bell pepper taste test, a recipe with peppers, and ways to connect peppers to the school cafeteria. ( HYPERLINK "http://www.sde.idaho.gov/site/cnp/ffvp/fruit_veg/Pepper - Bell.pdf" http://www.sde.idaho.gov/site/cnp/ffvp/fruit_veg/Pepper - Bell.pdf) A past Scientific American Frontiers episode (Lifes Little Questions, Show 904) included a segment on Why Are Peppers Hot? The supplementary materials for the show include a recipe for making capsaicin candy. Similar candies have been used to treat mouth sores that can occur during chemotherapy. Students could try such a recipe and taste the candies. ( HYPERLINK "http://www.pbs.org/safarchive/3_ask/archive/qna/3294_peppers.html" http://www.pbs.org/safarchive/3_ask/archive/qna/3294_peppers.html) Out-of-class Activities and Projects (student research, class projects) Students could visit local grocery and ethnic food stores to explore the variety of chili peppers available (both fresh and dried). The types could be listed in order of their pungency rating on the Scoville scale. Students could even try a few recipes that use chili peppers; the class could experience different levels of heat for themselves. Have a Chips and Salsa Day for real world experience with capsaicin. Prepare fresh salsa using different types of chili peppers and test the foods/drinks mentioned in the article for their ability to alleviate the capsaicin pain. Students could perform additional research on foods/drinks that potentially alleviate the pain, based on learning about the polarity of compounds in those foods/drinks. References (non-Web-based information sources) The ChemMatters article Pepper Power contrasts the ability of capsaicin to both cause pain through the use of pepper spray and relieve pain through the use of capsaicin in medications for the skin. (Williams, C. Pepper Power. ChemMatters 1995, 13 (2), pp 1013) Web sites for Additional Information (Web-based information sources) More sites on chili peppers Recent research discusses a possible link between personality and a preference for spicy foods such as chili peppers. ( HYPERLINK "http://news.psu.edu/story/141967/2012/06/27/research/some-it-hot" http://news.psu.edu/story/141967/2012/06/27/research/some-it-hot) A short report from 2012 discusses the Trinidad Moruga Scorpion pepper and its rise to the top of the list of hottest peppers. ( HYPERLINK "http://www.ibtimes.com/trinidad-moruga-scorpion-worlds-hottest-pepper-tops-2m-scoville-heat-units-411812" http://www.ibtimes.com/trinidad-moruga-scorpion-worlds-hottest-pepper-tops-2m-scoville-heat-units-411812) A post at the LiveScience Web site describes your bodys response if you eat a Trinidad Moruga Scorpion pepper and includes a video of someone eating an entire Scorpion pepper. ( HYPERLINK "http://www.livescience.com/34187-trinidad-moruga-scorpion-chili-pepper.html" http://www.livescience.com/34187-trinidad-moruga-scorpion-chili-pepper.html) A new champion? A September 2013 article in The Atlantic discusses the Carolina Reaper pepper in the cutthroat world of competitive chili pepper growing. ( HYPERLINK "http://www.theatlantic.com/health/archive/2013/09/so-god-made-the-worlds-hottest-pepper/279749/" http://www.theatlantic.com/health/archive/2013/09/so-god-made-the-worlds-hottest-pepper/279749/) A news clip discusses research into the use of peppers to treat cancer. The developer and grower of the Carolina Reaper pepper discusses his own use of peppers in the belief that they help to prevent cancer. ( HYPERLINK "http://www.cbsnews.com/8301-505269_162-57589329/" http://www.cbsnews.com/8301-505269_162-57589329/) As stated on its homepage, The Chile Pepper Institute is an international, non-profit organization devoted to education and research related to chile peppers. ( HYPERLINK "http://www.chilepepperinstitute.org/" http://www.chilepepperinstitute.org/) More sites on capsaicin A Royal Society of Chemistry podcast discusses capsaicin, including how it caused failed drug tests during the 2008 Olympicsin horses. ( HYPERLINK "http://www.rsc.org/chemistryworld/podcast/CIIEcompounds/transcripts/capsaicin.asp" http://www.rsc.org/chemistryworld/podcast/CIIEcompounds/transcripts/capsaicin.asp) The Royal Society of Chemistry article Spicing Up Chemistry discusses the effect capsaicin has on the body. It also shares information on other spices, such as turmeric, cinnamon, and ginger. ( HYPERLINK "http://www.rsc.org/Education/EiC/issues/2006May/SpicingupChemistry.asp" http://www.rsc.org/Education/EiC/issues/2006May/SpicingupChemistry.asp) Shimadzu, a scientific instrument company, shows several chromatograms obtained when analyzing capsaicinoids in pepper, pepper sauce, and spicy oil, using HPLC. ( HYPERLINK "http://www2.shimadzu.com/applications/lc/L335.pdf" http://www2.shimadzu.com/applications/lc/L335.pdf) A masters thesis presents background information about capsaicinoids and focuses on analytical techniques used with capsaicinoids. ( HYPERLINK "http://dc.etsu.edu/cgi/viewcontent.cgi?article=3152&context=etd&sei-redir=1&referer=" http://dc.etsu.edu/cgi/viewcontent.cgi?article=3152&context=etd&sei-redir=1&referer=) A high schools online newspaper describes the visit of an American Chemical Society chemistry textbook author to their high school for Chips and Salsa Day, where he presented a lecture on capsaicin. ( HYPERLINK "http://www.nshsdenebola.com/author-brings-chemistry-alive-on-chips-and-salsa-day/" http://www.nshsdenebola.com/author-brings-chemistry-alive-on-chips-and-salsa-day/) More sites on the Scoville heat scale The New Mexico State Universitys College of Agricultural, Consumer, and Environmental Sciences offers the publication Measuring Chile Pepper Heat, which includes a description of the Scoville Organoleptic Test. ( HYPERLINK "http://aces.nmsu.edu/pubs/_h/H237/" http://aces.nmsu.edu/pubs/_h/H237/) A biographical essay on Wilbur Scoville traces his career and describes how his research led him to develop the Scoville Organoleptic Test. ( HYPERLINK "http://www.fiery-foods.com/article-archives/85-chile-history/3171-wilbur-scoville-and-the-organoleptic-test-centennial" http://www.fiery-foods.com/article-archives/85-chile-history/3171-wilbur-scoville-and-the-organoleptic-test-centennial) Photographs of ten different varieties of chili peppers are shown with brief descriptions of each, from low to high on the Scoville scale. ( HYPERLINK "http://www.telegraph.co.uk/gardening/howtogrow/fruitandvegetables/10247130/Hot-stuff-top-10-chillies-in-order-of-heat.html" http://www.telegraph.co.uk/gardening/howtogrow/fruitandvegetables/10247130/Hot-stuff-top-10-chillies-in-order-of-heat.html) More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers) A 6-page teachers guide briefly describes the Scoville and HPLC techniques for determining the pungency of chilli peppers. It includes several questions related to capsaicinoids, the compounds that give chillies their heat, including their structure. ( HYPERLINK "http://www.rsc.org/learn-chemistry/resource/res00000820/kitchen-chemistry-how-hot-are-chilli-peppers?cmpid=CMP00000926" http://www.rsc.org/learn-chemistry/resource/res00000820/kitchen-chemistry-how-hot-are-chilli-peppers?cmpid=CMP00000926) Global Climate Change: A Reality Check Background Information (teacher information) More on the greenhouse effect and global warming In order to understand climate change, global warming or the greenhouse effect, students must understand the normal flow of energy through the Earths atmosphere. The energy that is radiated to the Earth comes, of course, from the sunan annual average of 240 watts of solar power per square meter. We know that in order to remain in a stable state, the Earth must also, then, redistribute that energy. Some of it is distributed from equator to poles, since the equator receives more direct radiation from the sun. Some of the energy is used in processes like photosynthesis and evaporation. The rest is re-radiated into the atmosphere and then back into space. As a result, the incoming and outgoing energy at the Earths surface is balanced. And under those conditions the average temperature of the Earth remains relatively stable. About 29 percent of the incoming solar radiation is reflected back into space and has no role to play in the Earth system. Of the other 71 percent that does enter the atmosphere, 23 percent is absorbed by water vapor, aerosols and ozone and 48 percent passes through the atmosphere and is absorbed by the Earths surface. Some of that energy is used to evaporate water as part of the natural hydrologic cycle and some of it drives convection currents. However, some of the incoming energy that is absorbed by the surface is reflected back through the atmosphere as infrared energy (heat) and into space. In general, the Earth maintains an energy balance in order to maintain a stable temperature. Note that there is a shift in the specific kind of electromagnetic energy from incoming to outgoing from the Earths surface. The electromagnetic energy flowing into the Earths atmosphere ranges from ultraviolet to the visible spectrum and infrared range. Energy coming from the sun to the Earth is in the shorter wave length rangevisible (0.4 to 0.7 m) and ultraviolet ranges. We know this intuitively because the sun lights our daytime hours and because in recent years there is increasing evidence that UV radiation causes skin cancer. The reflected or outgoing energy, on the other hand, is in the thermal IR range. See also  HYPERLINK "http://www.acs.org/content/acs/en/climatescience/energybalance.html" http://www.acs.org/content/acs/en/climatescience/energybalance.html. Some energy, then, passes through the gases in the atmosphere twice, incoming and outgoing. As noted above, the energy that the Earth reflects back into the atmosphere is in the infrared region of the energy spectrum. The gases that make up the bulk of the atmosphere, oxygen and nitrogen, do not absorb that reflected infrared thermal energy. We say that they are transparent to infrared thermal radiation. But other gases present in the atmosphere in lesser concentrationscarbon dioxide (CO2), methane (CH4), water vapor, nitrous oxide (N2O) and other trace gasesabsorb some of the out-going reflected energyabout 56 per cent of ittrapping it in the atmosphere, and thus preventing it from returning to space. These gas molecules, in turn, radiate energy out in all directions and in so doing increase the energy of nearby molecules. The net result is an increase in the kinetic energy of the molecules in the atmosphere and a consequent increase in the ambient temperature of the atmosphere. This is the greenhouse effect. Remember that this process has gone on for centuries. It is a natural process. The heat trapped in the atmosphere by gases we now call greenhouse gasesmainly carbon dioxide, methane, water vapor and nitrous oxide referenced aboveis a natural part of the Earths energy budget. In fact, without these heat-absorbing molecules the Earth would be an icy planet devoid of life. Remember also that these gases, especially carbon dioxide and water, are cycled in and out of the atmosphere naturally over shorter time periods and over centuries. Water vapor is cycled back to the Earths surface in liquid form by the hydrologic cycle. Carbon dioxide, produced and sent into the atmosphere by naturally-occurring oxidation, primarily as products of respiration and combustion, is cycled out of the atmosphere again by photosynthesis. Thus, carbon dioxide concentrations are at a maximum, at least in the northern hemisphere, in May when plants begin their growing cycle and reach a minimum in November at the end of the growing cycle. And there have been broader cyclic changes in average atmospheric CO2 concentration historically, as the graph below shows.  (from  HYPERLINK "http://www.ncdc.noaa.gov/paleo/icecore/antarctica/vostok/vostok_co2.html" http://www.ncdc.noaa.gov/paleo/icecore/antarctica/vostok/vostok_co2.html) The data from which this graph was created is the result of measuring CO2 concentrations in ice cores taken from Antarctic ice sheets. Air trapped in the ice is retrieved and various gas concentrations measured. The article describes this method of determining ancient temperatures. See More on measuring past climate conditions below. But on average the concentration of CO2 has remained relatively stable over centuries, thanks primarily to the photosynthesis-oxidation cycle. However, in recent decades the CO2 story has changed, as the graph below indicates.  INCLUDEPICTURE "http://www.esrl.noaa.gov/gmd/webdata/ccgg/trends/co2_data_mlo.png" \* MERGEFORMAT   According to NOAA: The carbon dioxide data (red curve), measured as the mole fraction in dry air, on Mauna Loa constitute the longest record of direct measurements of CO2 in the atmosphere. They were started by C. David Keeling of the Scripps Institution of Oceanography in March of 1958 at a facility of the National Oceanic and Atmospheric Administration [Keeling, 1976]. NOAA started its own CO2 measurements in May of 1974, and they have run in parallel with those made by Scripps since then [Thoning, 1989]. The black curve represents the seasonally corrected data. Data are reported as a dry mole fraction defined as the number of molecules of carbon dioxide divided by the number of molecules of dry air multiplied by one million (ppm). So, although the total concentration of heat-absorbing gases in the atmosphere has been relatively stable, on average, for centuries, recently the average CO2 concentrations have been steadily increasing. Most scientists attribute the increase to increased human activity, for example, in the form of burning fossil fuels which produces CO2. The reasoning goes that humans are pumping carbon dioxide into the atmosphere at a rate too fast for the Earths natural systems to cycle it out. And at the same time the rate of deforestation has been increasing, thus removing the trees that consume so much carbon dioxide for photosynthesis. The net effect is that heat-absorbing gases are being added to the Earths atmosphere as a result of human activity and that is happening faster than the Earth can remove them. Heat reflected from the Earth is, therefore, trapped in the atmosphere due to more molecules absorbing more heat, which contributes to the greenhouse effect, raising the temperature of the atmosphere and the Earths surface above natural levels, causing global warming. More on greenhouse gases The article explains briefly why some gases in the atmosphere absorb infrared energy and some do not. Recall from earlier in this Teachers Guide that the Earth absorbs and re-radiates some of the suns energy, and recall that the reflected energy is in the infrared range. If the only gases in the atmosphere were oxygen and nitrogen then all of the energy reflected by the Earth would travel back into space. But the atmosphere contains other gases like carbon dioxide and methane, and these gases absorb some of that heat and keep it in the atmosphere, producing the greenhouse effect. What makes carbon dioxide and methane different from oxygen and nitrogen? According to the National Oceanic and Atmospheric Administrations National Climatic Data Center: Many chemical compounds present in Earth's atmosphere behave as 'greenhouse gases'. These are gases which allow direct sunlight (relative shortwave energy) to reach the Earth's surface unimpeded. As the shortwave energy (that in the visible and ultraviolet portion of the spectra) heats the surface, longer-wave (infrared) energy (heat) is reradiated to the atmosphere. Greenhouse gases absorb this energy, thereby allowing less heat to escape back to space, and 'trapping' it in the lower atmosphere. Many greenhouse gases occur naturally in the atmosphere, such as carbon dioxide, methane, water vapor, and nitrous oxide, while others are synthetic. Those that are man-made include the chlorofluorocarbons (CFCs), hydrofluorocarbons (HFCs) and Perfluorocarbons (PFCs), as well as sulfur hexafluoride (SF6). Atmospheric concentrations of both the natural and man-made gases have been rising over the last few centuries due to the industrial revolution. As the global population has increased and our reliance on fossil fuels (such as coal, oil and natural gas) has been firmly solidified, so emissions of these gases have risen. While gases such as carbon dioxide occur naturally in the atmosphere, through our interference with the carbon cycle (through burning forest lands, or mining and burning coal), we artificially move carbon from solid storage to its gaseous state, thereby increasing atmospheric concentrations. ( HYPERLINK "http://www.ncdc.noaa.gov/cmb-faq/greenhouse-gases.php" http://www.ncdc.noaa.gov/cmb-faq/greenhouse-gases.php ) The difference at the molecular level is in the type and arrangement of bonds in each molecule. Your students will likely know that both oxygen and nitrogen, the atmospheric gases with the highest concentrations, are made up of diatomic molecules, O2 and N2. Recall that because of the symmetry of the two molecules, neither molecule is polar. In fact, because the two atoms in these two molecules are identical, there can be no dipole. In fact, only gas molecules with at least three atoms have the potential to be greenhouse gases. A word about dipole momentsthe bonds within a molecule consist of pairs of electrons. The arrangement of the bonds determines the distribution of electrons, or in other words, the distribution of negative charges in the molecule. If the charges are distributed in such way as to cancel each other out, the molecule has no electric dipole. That is, the molecule is not polarit has no permanent dipole. For example, in the BeF2 molecule which is linear in shape the two fluorine atoms are positioned on opposite sides of the beryllium. Each of the fluorine ions has a negative charge, but since they are directly opposite each other, they cancel each other and the molecule is nonpolar. Likewise, in a CCl4 molecule there are four chlorine atoms arranged in a tetrahedral shape around the central carbon. Each of the chlorine atoms is more electronegative than the carbon, so each of the individual bonds in the molecule is polar, but the arrangement of the bonds are symmetrical so the total molecule is not polar. It does not have a molecular dipole. As a third example, in a water molecule the two hydrogen atoms are on opposite sides of the central oxygen, but at an angle to each other. Since the oxygen is more electronegative it tends to have a more negative charge (because the electrons that form the bond are attracted more by the positive oxygen nucleus than by the hydrogen nuclei), and the two hydrogen atoms have a more positive charge. But since the two hydrogen atoms are arranged at an angle of 104.5 degrees to each other, there is a net negative charge in the direction of the oxygen and a net positive charge in the direction of the hydrogen atoms. That is, the water molecule has a dipole and is considered a polar molecule. In the water molecule described above the molecule has a permanent dipole. But it is possible to create a temporary dipole in a normally nonpolar molecule. Recall from kinetic theory that there are three types of molecular motiontranslation, rotation and vibration. Adding energylike the heat reflected from the Earth into the atmosphereto a molecule will cause the translational motion to increase. So the thermal IR radiation (heat) reflected from the Earth does, in fact, cause all the molecules in the atmosphere to move a little faster. As described above, this is important because this increased motion raises the temperature of the atmosphere sufficiently to enable life on Earth. Without this added energy the temperature of the atmosphere would be too cold for us to survive. However, the heat reflected from the Earth also causes some molecules to vibrate and rotate more than normal and more than other molecules. This occurs because the frequency of the normal molecular vibrations corresponds to the frequency of thermal IR radiation. As the molecules absorb this energy the pattern of electron distribution in the bonds changes. Bonds within the molecule may vibrate by stretching and recoiling like a rubber band or the bonds may be bent. As a result a temporary dipole is created in some molecules, and any molecule in which these changes occur vibrates or rotates a little more than normal. For example, according to the American Chemical Societys Climate Science Toolkit, in the carbon dioxide molecule the central carbon atom is . . . slightly positive . . . Since the molecule is linear with equal bond lengths, the center of negative charge and the center of positive charge coincide at the central point, the carbon atom, and the molecule has no permanent dipole moment. The symmetrical stretching vibration, top representation, does not change this symmetry, does not change the dipole moment, and does not lead to IR absorption. The molecular bending vibrations . . . displace the negative charges away from the line of centers of the molecule and create a structure with a dipole moment. This increased energy, in turn, is transferred to other molecules with which it collides, thus raising the temperature of the atmosphere. These kinds of gases are greenhouse gases. For much more detail on this warming mechanism see the American Chemical Societys Climate Science Toolkit at  HYPERLINK "http://www.acs.org/content/acs/en/climatescience/greenhousegases.html" http://www.acs.org/content/acs/en/climatescience/greenhousegases.html. As noted above, the main greenhouse gases are carbon dioxide (CO2), methane (CH4), water vapor, nitrous oxide (N2O). Also contributing to global warming are ozone and CFCs. Each of the gases, CFCs excepted, occurs naturally in the atmosphere. All are present in the atmosphere but at very different concentrations: Gas Concentration (ppb) Carbon dioxide 350,000 Methane 1,700 Nitrous oxide 0.001 to 50 CFCs 3 In addition to their concentrations in the atmosphere there are two other important factors related to greenhouse gases. The first is the average time each gas remains in the atmosphere. As noted above, for example, carbon dioxide is cycled in and out of the atmosphere by photosynthesis and oxidation. Each of the greenhouse gases has an average atmospheric lifetime: Gas Average Lifetime Carbon dioxide 100 years Methane 10 years Nitrous oxide days CFCs 60-100 years Remind students that these persistence figures tell us something about the chemical reactivity of the compounds. The less reactive a compound is the longer it remains in the atmosphere. This is one of the important intersections of climate change and chemistry. The longer a gas remains in the atmosphere the greater its influence. Note that even if efforts to eliminate the excess production of greenhouse gases were enacted now, the effect of these gases would continue well into the future. The second factor to be considered when rating the influence of greenhouse gases is the degree to which they absorb the infrared radiation passing through the atmosphere. Some gases absorb IR radiation much better than others. That is, some gases are more efficient at absorbing IR radiation. So each of the greenhouse gases is assigned an efficiency value called the Global Warming Potential (GWP), which is a comparison of the efficiency of a gas relative to CO2 over a time span of 100 years. For example, a gas with a GWP of 20 is 20 times more efficient at retaining heat than CO2 over a 100 year time period. Gas GWP Carbon dioxide 1 Methane 25 Nitrous oxide 298 CFCs 10,900 So, although CO2 is the most concentrated of the greenhouse gases, its IR-absorbing efficiency is lowest of the gases. And even though CFCs are 10,900 times more efficient at absorbing IR their concentration is very low. Lets take a closer look at each of the gases that play an important role in global warming. Water VaporYou will note that water vapor is missing from the lists above even though it is the most abundant greenhouse gas. The role of water vapor in global warming is not well understood. This explanation comes for the National Oceanic and Atmospheric Administrations National Climatic Data Center: Water Vapor is the most abundant greenhouse gas in the atmosphere, which is why it is addressed here first. However, changes in its concentration are also considered to be a result of climate feedbacks related to the warming of the atmosphere rather than a direct result of industrialization. The feedback loop in which water is involved is critically important to projecting future climate change, but as yet is still fairly poorly measured and understood. As the temperature of the atmosphere rises, more water is evaporated from ground storage (rivers, oceans, reservoirs, soil). Because the air is warmer, the absolute humidity can be higher (in essence, the air is able to 'hold' more water when it's warmer), leading to more water vapor in the atmosphere. As a greenhouse gas, the higher concentration of water vapor is then able to absorb more thermal IR energy radiated from the Earth, thus further warming the atmosphere. The warmer atmosphere can then hold more water vapor and so on and so on. This is referred to as a 'positive feedback loop'. However, huge scientific uncertainty exists in defining the extent and importance of this feedback loop. As water vapor increases in the atmosphere, more of it will eventually also condense into clouds, which are more able to reflect incoming solar radiation (thus allowing less energy to reach the Earth's surface and heat it up). The future monitoring of atmospheric processes involving water vapor will be critical to fully understand the feedbacks in the climate system leading to global climate change. As yet, though the basics of the hydrological cycle are fairly well understood, we have very little comprehension of the complexity of the feedback loops. Also, while we have good atmospheric measurements of other key greenhouse gases such as carbon dioxide and methane, we have poor measurements of global water vapor, so it is not certain by how much atmospheric concentrations have risen in recent decades or centuries, though satellite measurements, combined with balloon data and some in-situ ground measurements indicate generally positive trends in global water vapor. ( HYPERLINK "http://www.ncdc.noaa.gov/cmb-faq/greenhouse-gases.php" http://www.ncdc.noaa.gov/cmb-faq/greenhouse-gases.php) Carbon dioxideCO2 is responsible for at least 60% of the greenhouse effect. Prior to the Industrial revolution CO2 concentrations were relatively stable at about 280 ppm. Currently it is in the range of 370390 ppm, an increase of 30%. It was the first greenhouse gas whose concentration in the atmosphere was actually measured in the 1950s. Sources of CO2 are shown by the chart at right. The increasing concentration is the result of increased combustion of fossil fuels (carbon dioxide is one of the products) and the decrease in trees and other biomass capable of photosynthesis. As noted above, CO2 concentrations vary cyclically in the Northern Hemisphere as a result of plant growth cycle beginning in the spring of the year and ending in the fall. Concentrations begin to decrease in the spring and increase in the fall. It was noted above that the concentration of CO2 in the atmosphere is increased by combustion and other forms of oxidation and decreased by photosynthesis. In general, regions or processes that predominately produce CO2 are called sources of atmospheric CO2, while those that absorb CO2 are called sinks. Sources and sinks are part of the natural carbon cycle. See More on climate change solutions, below, for details. Another important carbon sink is the ocean since CO2 is soluble in water, forming the weak acid carbonic acid as well as bicarbonates and carbonates. The concentration of carbon dioxide in the ocean is estimated to be 50 times that of the atmosphere. The ocean dissolves about a third of all CO2 emitted into the atmosphere. This is beneficial for the atmosphere but harmful to the ocean since the increase in dissolved CO2 results in a lowered pH for the oceans. This, in turn affects marine biological systems negatively. In its 2013 State of the Science Fact Sheet on Ocean Acidification (OA), NOAA indicates that: The geological record reveals several acidification events in the distant past which limited the abundance, diversity, and evolution of calcifying organisms throughout the worlds oceans. Laboratory and field studies help scientists better understand the implications of modern OA resulting from human activities. These studies demonstrate that many marine species will likely experience adverse effects on health, growth, reproduction, and survival particularly in early life-stages. Most species of coral, calcifying algae, coccolithophores, and molluscs (including some economically important oysters), calcify slower under OA. The effect of OA on marine crustaceans (e.g., copepods, crabs, lobsters, crayfish, shrimp, krill and barnacles) is inconclusive at this time. However, decreases in survival, growth rate, and egg production have been reported for some species. Effects on non-calcifying organisms also have been demonstrated, including on the development of larval stages of some fish and on the ability to detect predators. Reduced survival and growth of sea urchins, sand dollars, seastars, sea cucumbers, and brittlestars may also occur. Some phytoplankton and seagrasses may benefit from OA, likely furthering shifts in community composition. ( HYPERLINK "http://www.noaa.gov/factsheets/new%20version/SoS%20Fact%20Sheet_Ocean%20Acidification%2020130306%20Final.pdf" http://www.noaa.gov/factsheets/new%20version/SoS%20Fact%20Sheet_Ocean%20Acidification%2020130306%20Final.pdf) In the oceans some carbon dioxide is used by phytoplankton and algae through the process of photosynthesis. Also in the oceans organisms like coral and those organisms with shells take up carbon dioxide and convert it to calcium carbonate for their shells. As the organisms die the carbonate falls to the bottom of the ocean as sediment, forming limestone rock which can be redissolved releasing carbon dioxide back into the water and perhaps the air. Other sources of CO2 are volcanic activity, hydrothermal vents and hot springs. To view a short video on the basics of the carbon cycle, see  HYPERLINK "http://epa.gov/climatestudents/basics/today/carbon-dioxide.html" http://epa.gov/climatestudents/basics/today/carbon-dioxide.html. MethaneMethane gas is present in the atmosphere as the result of natural biological processes like the anaerobic decomposition of organic material in rice fields. This decomposition is called methanogenesis, and more than 100 million tons of rice production annually produces significant amounts of the gas, most of which is emitted into the atmosphere. Other sources of the gas include cattle ranching, coal mining and increased use of natural gas. Direct atmospheric measurement of atmospheric methane has been possible since the late 1970s and its concentration rose from 1.52 ppmv (parts per million by volume) in 1978 by around 1 percent per year to 1990 when its concentration leveled off at 1.77 ppmv. The concentration of methane in the atmosphere is less than 1% of the carbon dioxide concentrations, but methane is 25 times more effective at absorbing infrared radiation so it should be considered an important factor in global warming. Estimates indicate that it accounts for about 10% of the greenhouse effect. Nitrous OxideIncreased use of fertilizer is the main cause for the increase in N2O concentration in the atmosphere since the beginning of the Industrial Revolution. Concentration for N2O in 1998 was 314 ppb, making it responsible for 6% of the greenhouse effect. In addition to agricultural sources, some industrial processes like fossil fuel-fired power plants, nylon production, nitric acid production and vehicle emissions are also sources. Nitrous oxide is 230 times better at absorbing IR radiation than CO2. CFCsThe only major greenhouse gases that are not naturally occurring are the chlorofluorocarbons. They come from sources such as the production and/or use of foams, aerosols, refrigerants, and solvents. They are present at an extremely low concentration in the atmosphere, but they are 15,000 times more efficient as a greenhouse gas relative to carbon dioxide. As a result they contribute to approximately 25% of the total greenhouse effect based on 1990 concentrations. They were first produced in large quantities in the late 1920s and, since then, concentrations of CFCs in the atmosphere have been rising. Due to the discovery that they are able to destroy stratospheric ozone, major international initiatives are under way to restrict their production and use. However, their long atmospheric lifetimes mean that some concentration of the CFCs will remain in the atmosphere for over 100 years. Other GasesThere are three other gases that are relateddirectly or indirectlyto the greenhouse effect. They are ozone, carbon monoxide and volatile organic compounds. Even though increased ozone concentrations appear primarily in the Northern Hemisphere, near cities, and are cyclic in nature, ozone is considered by the International Panel on Climate Change to be an important greenhouse gas. Ozone concentrations have risen by 30% since the Industrial Revolution. Carbon monoxide (CO) is not considered a greenhouse gas, but it affects the production of both methane and ozone, themselves greenhouse gases. In addition, VOCs are also instrumental in the production of ozone. The table below summarizes the major greenhouse characteristics, sources and concentrations of greenhouse gases. Gas Major Anthropogenic Sources Amount Released per Year (millions of tons) Average Time in the Atmosphere Global Warming Potential* (over 100 years) Pre-industrial Concentration (around 1860) (ppb) Average Concentration now (ppb) Expected Concentration in 2030 (ppb) CO2 Burning of Fossil Fuels 5,500 100 years 1 290,000 350,000 500,000 CH4 Fossil Fuel Production, Rice Fields 500 10 years 21 850 1,700 2,300 N2O Fertilizers, Deforestation, Burning Biomass 30 Days 310 .001 to 7 .001 to 50 .001 to 50 CFCs Aerosol Sprays, Refrigerants 1 60 to 100 years 1500-8100 0 about 3 2.4 to 6  (from  HYPERLINK "http://telstar.ote.cmu.edu/environ/m1/s2/subsect/ghgs.htm" http://telstar.ote.cmu.edu/environ/m1/s2/subsect/ghgs.htm) More on climate change The Teachers Guide for the article on Mt. Kilimanjaro (see References, below) says about climate change that: Climate change refers to the long-term shift in average weather as a result of changes in the atmosphere-ocean-land system that affects a regions weather. Long-term changes in climate are actually normal. The Cretacous period (120 million-90 million years ago) in North America was marked by vegetation that grows only in warm climates and the age of dinosaurs. On the other hand, massive ice sheets prevailed about 21,000 years ago. In the last 650,000 years there have been seven cycles in which glaciers have advanced and retreated. These variations took place over long time periods and over large land masses. Students should not confuse climate change with unusual isolated local weather events, like snow in normally balmy regions. ( HYPERLINK "http://www.acs.org/content/dam/acsorg/education/resources/highschool/chemmatters/chemmatters-feb2011-tg-pdf.pdf" http://www.acs.org/content/dam/acsorg/education/resources/highschool/chemmatters/chemmatters-feb2011-tg-pdf.pdf) So we know that the gases described in the previous section of this Teachers Guide are present in the Earths atmosphere in increasing concentrations. And we know that these gases trap heat to a greater extent than normal and that on average the Earths temperature has risen about 1.4 oF in the last century. The central question is this: Is the Earths climate changing as a result? Before we get to that issue, a word or two about weather vs. climate. Weather is a description of the conditions in the atmosphere at a given time and place. Most of what we call weather takes place near the surface of the Earth in the troposphere. When we think of weather we think of temperature, precipitation, clouds and wind, for example. Climate, on the other hand, is a description of atmospheric conditions over longer periods of time and over larger geographic areas. While weather changes frequently climate tends to remain on average fairly stable unless some force or forceslike large volcanic eruptions or significant changes in greenhouse gas concentrationsshift the climate. Climate change, then, refers to any significant change in the measures of climate lasting for an extended period of time. In other words, climate change includes major changes in temperature, precipitation, or wind patterns, among other effects, that occur over several decades or longer. What are these indicators of climate change and what is their status? The EPA lists 26 of these climate change indicators. Below are brief summaries of the data for the twenty indicators that are related to physical factors in the environment. For the complete listing and extensive graphs that accompany the data, see  HYPERLINK "http://www.epa.gov/climatechange/science/indicators/" http://www.epa.gov/climatechange/science/indicators/. References to charts and graphs in the summaries that follow have been omitted. U.S. Greenhouse Gas Emissions - In 2011, U.S. greenhouse gas emissions totaled 6,702 million metric tons of carbon dioxide equivalents, an 8 percent increase from 1990. Emissions of carbon dioxide, the primary greenhouse gas emitted by human activities, increased by 10 percent. Global Greenhouse Gas Emissions - Between 1990 and 2005, global emissions of all major greenhouse gases increased. Emissions of carbon dioxide increased by 31 percent, which is particularly important because carbon dioxide accounts for nearly three-fourths of total global emissions. Methane emissions increased the least10 percentwhile emissions of nitrous oxide increased by 14 percent. Emissions of fluorinated gases more than doubled. In 2005, estimated worldwide emissions totaled nearly 39 billion metric tons of greenhouse gases, expressed as carbon dioxide equivalents. This represents a 26 percent increase from 1990. Atmospheric Concentrations of Greenhouse Gases - Global atmospheric concentrations of carbon dioxide, methane, nitrous oxide, and certain manufactured greenhouse gases have all risen over the last few hundred years. Before the industrial era began in the late 1700s, carbon dioxide concentrations measured approximately 280 ppm. Concentrations have risen steadily since then, reaching an annual average of 394 ppm in 2012a 41 percent increase. Almost all of this increase is due to human activities. The concentration of methane in the atmosphere has more than doubled since preindustrial times, reaching about 1,826 ppb in 2012. It is very likely that this increase is predominantly due to agriculture and fossil fuel use. Climate Forcing - In 2011, the Annual Greenhouse Gas Index was 1.30, an increase in radiative forcing [Radiative forcing is any change in the Earths energy flow as a result of changing ofany physical factor, in this case, increase in greenhouse gas concentrations.] of 30 percent since 1990. Of the greenhouse gases, carbon dioxide accounts for by far the largest amount of radiative forcing, and its contribution continues to grow at a steady rate. By 2011, radiative forcing due to carbon dioxide was 40 percent higher than in 1990. Carbon dioxide accounts for approximately 80 percent of the overall increase in radiative forcing since 1990. [For more on climate forcing, see  HYPERLINK "http://ossfoundation.us/projects/environment/global-warming/radiative-climate-forcing" http://ossfoundation.us/projects/environment/global-warming/radiative-climate-forcing .] U.S. and Global Temperatures - Since 1901, the average surface temperature across the contiguous 48 states has risen at an average rate of 0.14F per decade (1.4F per century). Average temperatures have risen more quickly since the late 1970s (0.36 to 0.55F per decade). Seven of the top 10 warmest years on record for the contiguous 48 states have occurred since 1998, and 2012 was the warmest year on record. High and Low Temperatures - Unusually hot summer days (highs) have become more common over the last few decades The occurrence of unusually hot summer nights (lows) has increased at an even faster rate. This trend indicates less "cooling off" at night. The 20th century saw many winters with widespread patterns of unusually low temperatures, including a particularly large spike in the late 1970s Since the 1980s, though, unusually cold winter temperatures have become less commonparticularly very cold nights (lows). U.S. and Global Precipitation - On average, total annual precipitation has increased over land areas in the United States and worldwide. Since 1901, global precipitation has increased at an average rate of 2.2 percent per century, while precipitation in the contiguous 48 states has increased at a rate of 5.0 percent per century. Heavy Precipitation - The prevalence of extreme single-day precipitation events remained fairly steady between 1910 and the 1980s, but has risen substantially since then. Over the entire period from 1910 to 2012, the portion of the country experiencing extreme single-day precipitation events increased at a rate of about half a percentage point per decade (5 percentage points per century) Drought - Over the period from 2000 through 2012, roughly 30 to 70 percent of the U.S. land area experienced conditions that were at least abnormally dry at any given time The years 2002, 2003, 2007, and 2012 were relatively high drought years, while 2001, 2005, 2009, and 2010 were relatively low drought years. Tropical Cyclones - Since 1878, about six to seven hurricanes have formed in the North Atlantic every year. Roughly two per year make landfall in the United States. The total number of hurricanes (particularly after being adjusted for improvements in observation methods) and the number reaching the United States do not indicate a clear overall trend since 1878. Ocean Heat - In three different data analyses, the long-term trend shows that the oceans have become warmer since 1955. Sea Surface Temperatures - Sea surface temperature increased over the 20th century and continues to rise. From 1901 through 2012, temperatures rose at an average rate of 0.13F per decade. Sea Level - After a period of approximately 2,000 years of little change, global average sea level rose throughout the 20th century, and the rate of change has accelerated in recent years. When averaged over all the world's oceans, absolute sea level increased at an average rate of 0.07 inches per year from 1880 to 2011. From 1993 to 2011, however, average sea level rose at a rate of 0.11 to 0.13 inches per yearroughly twice as fast as the long-term trend. Ocean Acidity - Measurements made over the last few decades have demonstrated that ocean carbon dioxide levels have risen in response to increased carbon dioxide in the atmosphere, leading to an increase in acidity (that is, a decrease in pH) Arctic Sea Ice September, 2012, had the lowest sea ice extent on record,49 percent below the 1979-2000 average for that month. Glaciers - On average, glaciers worldwide have been losing mass since at least the 1970s which in turn has contributed to observed changes in sea level. Measurements from a smaller number of glaciers suggest that they have been shrinking since the 1940s. The rate at which glaciers are losing mass appears to have accelerated over roughly the last decade. Lake Ice - The time that lakes stay frozen has generally decreased since the mid-1800s. For most of the lakes in this indicator, the duration of ice cover has decreased at an average rate of one to two days per decade. Snowfall - Total snowfall has decreased in most parts of the country since widespread observations became available in 1930, with 57 percent of stations showing a decline. Snow Cover - Looking at averages by decade suggests that the extent of North America covered by snow has decreased somewhat over time. The average extent for the most recent decade (20032012) was 3.18 million square miles, which is 4 percent (132,000 square miles) smaller than the average extent during the first 10 years of measurement (19721981). Snowpack - From 1950 to 2000, April snowpack declined at most of the measurement sites with some relative losses exceeding 75 percent. ( HYPERLINK "http://www.epa.gov/climatechange/science/indicators/" http://www.epa.gov/climatechange/science/indicators/) As you discuss this article with students, it is important to reference the data that is being accumulated to support the reality of climate change. You will notice that each data set above and the data in the original EPA source may not provide conclusive evidence for climate change, but the accumulation of data paints a fairly conclusive picture of climate change. More on measuring past climate conditions The article briefly mentions how scientists are able to determine climate conditions in the ancient past, long before any actual measurements were recorded. The study of past climates is known as paleoclimatology. Climatologists divide climate study into three time periods that are based on the kind of data used to study climate change. The instrumental era includes the last 150 years when accurate weather records are available. The historical era includes the records from all of human history. Much of the information available from this time period is not very precise nor even quantitative, but it is actual data. The pre-historic era (or the period of paleoclimatology) relies on proxy data to infer changes in climate. The time scale used when talking about climate change is also important. There are long-term trends in climate as well as shorter periods of change. Climatologists usually use four time periods: Long term- Hundreds of millions of years; Medium term- One million years; Short term- ~160,000 years; Modern period- Hundreds of years. ( HYPERLINK "http://www.acs.org/content/dam/acsorg/education/resources/highschool/chemmatters/chemmatters-feb2011-tg-pdf.pdf" http://www.acs.org/content/dam/acsorg/education/resources/highschool/chemmatters/chemmatters-feb2011-tg-pdf.pdf) The article mentions the study of ice core samples as one method of determining the composition of the atmosphere over time. The air samples trapped in the ice can be analyzed and from that analysis scientists can determine the composition of the atmosphere at a particular point in history. You might mention to students as part of this discussion that the gas analysis is done primarily by mass spectrometry. A mass spectrograph works this way: molecules in question are inserted into the instrument, ionized, accelerated to the same velocities and then acted upon by a magnetic or electric field. The magnetic field deflects the moving particles, the lighter ones being deflected to a greater degree than the heavier ones. So molecules and parts of molecules are separated according to their masses. To see how the mass spectrometry works consider the analysis of water vapor tapped in an ice-encased air bubble. The oxygen component of water exists in two isotopic formsO-16 and O-18. Remember that each oxygen atom has eight protons. Most (by far) oxygen atoms also have eight neutrons. Thats O-16. But a small percent of oxygen atoms have ten protons. Thats O-18, the heavier isotope. When the water vapor is analyzed in the mass spectrometer, the O-16 water molecules are deflected to a great degree than the O-18 molecules. The O-18 water is heavier (because the O-18 isotope is heavier than the O-16 isotope). We know that the O-16 water has a higher vapor pressure and a slightly lower boiling point than water composed of O-18, and, therefore, it evaporates more easily than O-18 water. So, as past climate temperatures increased, the O-16 molecules evaporated to a greater degree, leaving a slightly increased proportion of O-18 molecules in the water of oceans and lakes. Thus, when chemists examine ice cores and determine the concentration of oxygen isotopes, a higher O-18 to O-16 ratio means that the climate was warmer and a lower ratio means the climate was cooler. You can read a brief description of mass spectrometry here:  HYPERLINK "http://masspec.scripps.edu/mshistory/whatisms_toc.php" http://masspec.scripps.edu/mshistory/whatisms_toc.php. Another method of analysis is gas chromatography, which is summarized here:  HYPERLINK "http://chemwiki.ucdavis.edu/Analytical_Chemistry/Instrumental_Analysis/Chromatography/Gas_Chromatography" http://chemwiki.ucdavis.edu/Analytical_Chemistry/Instrumental_Analysis/Chromatography/Gas_Chromatography. There are other proxy methods used by paleoclimatologists. Some of them were listed in the Teachers Guide for the article on Mt. Kilimanjaro (see References, below). For assessing the history of climate changes we must rely upon "proxy" climate indicators--natural archives that record seasonal or annual climate conditions such as ice cores, tree-ring measurements, laminated sediments, microbial life and corals--combined with the relatively small amount of available historical documentary or instrumental evidence available in prior centuries. Paleoclimatologists gather data from these natural recorders of climate variability, and by analyzing records taken from these and other sources, scientists can extend our understanding of climate far beyond the 100+ year instrumental record. Listed below are some widely used proxy climate data types: Ice Cores: Scientists drill into ice sheets and collects long cylinders of ice called cores. These cores contain dust, pollen and gases that can be translated into a history of climate in the region. Tree Rings: Scientists study the concentric rings that result from the annual growth of trees. The rings have varying widths and density and contain different elemental isotopes depending on climate conditions. Corals: Much like tree growth, corals exhibit annual variations in the thickness and density of their skeletons. The skeletons are made of calcium carbonate. In addition, the oxygen isotope content of the CaCO3 will vary depending on the temperature of the ocean. Depending on prevailing temperatures, the oxygen in the carbonate may be composed of varying ratios of O-16 or O-18. Fossil Pollen: The pollen from flowering plants is deposited in the sediment at the bottom of a body of water. From the type of pollen scientists can infer the type of plants living at the time the sediment was created and thereby gain a clue to the climate of that period. Ocean & Lake Sediments: Scientists take core samples of ocean and lake sediments. In addition to pollen, fossils and chemicals embedded in the sediment are analyzed to determine the climate in the past. Microbial Data: Foraminifera and diatoms are commonly used microbial climate proxies. Both are aquatic organisms with shells. Foraminifera shells are made up of calcium carbonate (CaCO3) and diatom shells are composed of silicon dioxide (SiO2). In warmer water environments, the shells will be richer in O-18 and in colder conditions, O-16 will prevail. Paleoclimatologists weave together the data from all of these sources in an attempt to create a history of the Earths climate. Proxy records are coordinated with current data and the historical record, usually using computer models. They also use the models to predict future climate. More on climate change effects There are many, many ways in which climate change will impact the Earth. The article describes some of these effects in the opening paragraphs, including temperature and sea level increases, more frequent severe storms, hurricanes in new parts of the world, more frequent heat waves and animals migrating to new habitats. And theres more. According to National Geographic, for example: Some impacts from increasing temperatures are already happening. Ice is melting worldwide, especially at the Earths poles. This includes mountain glaciers, ice sheets covering West Antarctica and Greenland, and Arctic sea ice. Researcher Bill Fraser has tracked the decline of the Adlie penguins on Antarctica, where their numbers have fallen from 32,000 breeding pairs to 11,000 in 30 years. Sea level rise became faster over the last century. Some butterflies, foxes, and alpine plants have moved farther north or to higher, cooler areas. Precipitation (rain and snowfall) has increased across the globe, on average. Spruce bark beetles have boomed in Alaska thanks to 20 years of warm summers. The insects have chewed up 4 million acres of spruce trees. Other effects could happen later this century, if warming continues. Sea levels are expected to rise between 7 and 23 inches (18 and 59 centimeters) by the end of the century, and continued melting at the poles could add between 4 and 8 inches (10 to 20 centimeters). Hurricanes and other storms are likely to become stronger. Species that depend on one another may become out of sync. For example, plants could bloom earlier than their pollinating insects become active. Floods and droughts will become more common. Rainfall in Ethiopia, where droughts are already common, could decline by 10 percent over the next 50 years. Less fresh water will be available. If the Quelccaya ice cap in Peru continues to melt at its current rate, it will be gone by 2100, leaving thousands of people who rely on it for drinking water and electricity without a source of either. Some diseases will spread, such as malaria carried by mosquitoes. Ecosystems will changesome species will move farther north or become more successful; others wont be able to move and could become extinct. Wildlife research scientist Martyn Obbard has found that since the mid-1980s, with less ice on which to live and fish for food, polar bears have gotten considerably skinnier. Polar bear biologist Ian Stirling has found a similar pattern in Hudson Bay. He fears that if sea ice disappears, the polar bears will as well. ( HYPERLINK "http://environment.nationalgeographic.com/environment/global-warming/gw-effects" http://environment.nationalgeographic.com/environment/global-warming/gw-effects) The EPA lists multiple categories of changes that are occurring or are predicted to occur. They include changes in agriculture. For example, some crops may benefit from increased carbon dioxide in the atmosphere, but the frequency of droughts will clearly have negative impacts on crop yields. Growing seasons may change, and these kinds of changes will change the worlds food supply. Forest growth may also be affected. Increased CO2 levels would benefit forest, but without adequate rainfall (in the western parts of the U.S., for example), the benefits will be negated. Droughts will also change the food supply for livestock, and may also increase the prevalence of livestock disease. Ecosystems will also be affected. Changes in temperature may force some species to move to more northerly latitudes or higher altitudes for cooler temperatures. Sea level rise may force saline water into formerly fresh water regions. Warmer springs may change the nesting periods for birds. Food webs will be altered as a result of these changes. The physical infrastructure may be affected as well. For example, increased temperatures will have negative effects on roadways and bridges, causing them to erode more quickly. In some parts of the country, however, roadways may be positively affected. Consider regions that typically get large amounts of snow each winter. If climate change decreases snowfall, communities will use less de-icers on roads thus decreasing the rates of erosion. The examples above are just a few of the possible effects of climate change. A more complete description from the EPA appears here:  HYPERLINK "http://www.epa.gov/climatechange/impacts-adaptation/" http://www.epa.gov/climatechange/impacts-adaptation/. More on climate change solutions  There are two ways to look at how we can mitigate global warming/climate change. There are large-scale technological fixes and there are ways in which individuals can lessen the effects of climate change. But first remember that the Earth has its own methods of trying to keep the atmospheric carbon dioxide concentration in balancethe carbon cycle. Carbon is cycled through the environment by means of chemical and physical changes. Two of those processes, already referenced, are photosynthesis and combustion/ respiration (oxidation). The former process removes CO2 from the atmosphere and the latter returns it to the atmosphere. Carbon dioxide is also soluble in water, a process that removes the gas from the atmosphere. The diagram at right illustrates these and allied processes that are part of the natural carbon cycle. The photosynthesis reaction, the process by which green plants remove CO2 from the atmosphere looks like this: 6 CO2 + 6 H2O + Energy ' C6H12O6 + 6 O2 So we can think of plants world-wide as natural carbon sinks, which have been mentioned in passing, above. Carbon sinks are any material or process that absorbs more carbon dioxide than it produces. World-wide, plants absorb about 2 billion tons of CO2 each year, about one quarter to one third of the gas that is produced by human activity. As organic plant matter dies and decays, the carbon contained in the matter is stored in the soil, and scientists estimate that the soil stores about 2 trillion tons of carbon. Plants, then, are significant carbon sinks, and monitoring, maintaining and replenishing trees contributes to a reduction in carbon dioxide in the atmosphere and a consequent reduction in global warming. A second major natural carbon sink is the ocean. The oceans take up a quarter of the CO2 produced by human activity. Carbon dioxide is soluble in water. As you can see from the graph at right, CO2 is much more soluble in water than oxygen. So carbon dioxide dissolves in the oceans to a great degree, removing it from the atmosphere. At some point there is an equilibrium established between the carbon dioxide dissolving and the CO2 coming out of solution. The equilibrium is determined in part by the partial pressure of CO2 in the atmosphere. So the greater the CO2 concentration in the atmosphere the more of it dissolves. As CO2 concentration increases in the atmosphere as a result of human activity more CO2 will tend to dissolve in the ocean. And current estimates indicate that once CO2 dissolves in the ocean it will remain there for as long as 500 years. (from  HYPERLINK "http://www.elmhurst.edu/~chm/vchembook/174temppres.html" http://www.elmhurst.edu/~chm/vchembook/174temppres.html) However, the increasing CO2 concentration in the atmosphere is also causing a rise in temperature, and this factor tends to decrease the solubility of CO2 in the ocean since we know that gases are less soluble as temperature increases. In addition, it is the warmer water at the surface of the ocean that interacts with atmospheric CO2, limiting the solubility of the gas. Not until the warmer surface water can be very slowly mixed with the deeper cooler water can the rate of dissolving be increased. Ocean water mixing occurs slowly over years, and if the rate at which CO2 is being pumped into the atmosphere is increasing (as it is) then the net outcome is still an increase in atmospheric CO2. Nevertheless, the worlds oceans, like plants and soil are natural reservoirs of stored carbon dioxide. So there are natural reservoirs that can store carbon dioxide. But scientists have been looking for technological ways to capture CO2 and store it. These processes are called carbon sequestration. According to the EPA: Carbon dioxide (CO2) capture and sequestration (CCS) is a set of technologies that can greatly reduce CO2 emissions from new and existing coal- and gas-fired power plants and large industrial sources. CCS is a three-step process that includes: Capture of CO2 from power plants or industrial processes Transport of the captured and compressed CO2 (usually in pipelines). Underground injection and geologic sequestration (also referred to as storage) of the CO2 into deep underground rock formations. These formations are often a mile or more beneath the surface and consist of porous rock that holds the CO2. Overlying these formations are impermeable, non-porous layers of rock that trap the CO2 and prevent it from migrating upward. ( HYPERLINK "http://www.epa.gov/climatechange/ccs/" http://www.epa.gov/climatechange/ccs/) The EPA diagram below illustrates the way in which sequestration is accomplished at an electric power plant site. Electric power plants produce nearly 40% of emitted CO2.  INCLUDEPICTURE "http://www.epa.gov/climatechange/images/ccs/CCS_Earth_Layers_Graphic_v6.JPG" \* MERGEFORMATINET  A note about sequestration languagethe natural methods of carbon dioxide storage, like oceans and plants, are usually referred to as biologic or terrestrial sequestration. The technological methods are called geologic sequestration. The sidebar in the article also describes ways that individuals can reduce the effects of climate change. For example, the article says to take public transportation whenever possible. How will that reduce the effects of climate change? When private cars burn gasoline one of the resulting products of the combustion reaction is carbon dioxide: 2 C8H18 + 13 O2 ' 16 CO2 + 18 H2O Therefore, reducing the use of private vehicles by riding public transportation also produces less CO2 and thus mitigates climate change. Using examples like this connects the chemistry concepts to the climate change issue. Another example of individual action is retrofitting homes for energy efficiency. If homes are heated with oil or natural gas, one of the combustion products will be carbon dioxide. If homes are heated by electricity you can note to students that most electricity is produced in the U.S. by the combustion of coal or petroleum, and so the CO2 reduction solution still applies. By increasing building insulation we decrease the fuel consumed and as a result decrease the carbon dioxide emitted. The U.S. Environmental Protection Agency has a detailed guide to the ways in which individuals can reduce greenhouse gas emissions:  HYPERLINK "http://www.epa.gov/climatechange/wycd/index.html" \t "_blank" http://www.epa.gov/climatechange/wycd/index.html, and an online calculator to help you estimate your own greenhouse gas emissions:  HYPERLINK "http://www.epa.gov/climatechange/wycd/calculator/ind_calculator.html" \t "_blank" http://www.epa.gov/climatechange/wycd/ calculator/ind_calculator.html. See More on climate change solutions for Web sites that provide added examples. Connections to Chemistry Concepts (for correlation to course curriculum) Interactions of matter and energyThe greenhouse effect is the result of the interaction between long wave length infrared radiation and molecules like CO2, H2O and other gases. Chemical bondsThe nature of chemical bonds in gas molecules determine whether the gas will be a greenhouse gas. Chemistry and the environmentThis article provides an opportunity to discuss with students the role that chemistry plays in environmental concerns. Physical changeSome of the changeslike increased flooding or the ocean as a carbon sinkinvolve physical changes, like change of phase or dissolving. Electromagnetic spectrumStudents will need to understand the basics of the electromagnetic spectrum in order to understand the greenhouse effect. Matter cycles and energy flowThe ways in which energy undergoes conversions and the way matter undergoes a series of cyclic chemical changes are important concepts in this article. Chemical changesFundamental to climate change is an understanding of chemical changes that produce or consume carbon dioxide and other greenhouse gases. Analytical chemistryDetermining the composition of the atmosphere and the analysis of temperature proxies like air in ice core samples demonstrates the importance of chemical analysis. Possible Student Misconceptions (to aid teacher in addressing misconceptions) Recent global warming is caused by the sun. The suns energy reaching the Earth has been measured for thirty years and it is not increasing. Measurements made by satellites indicate that the amount of sunlight reaching the Earth varies by about 0.1 percent over an 11 year cycle. This variation causes a temperature variation on earth of less than 0.2 oF, much less than the 1.2 oF increase over the last 50 years. The greenhouse effect is a negative factor in our environment. Not completely true. Were it not for greenhouse gases, our atmosphere would have an average temperature of -22 oF. So the greenhouse gases that are the result of natural phenomena are essential for life on Earth. It is the increased concentration of those gases that is causing global warming, and that is a negative environmental factor. Ive read that the Earth has experienced major changes in climate before, so whats going on now is just one more natural change. Why should we worry? While it is true that there have been major climate shifts in the past, they were the result of natural conditions. We have evidence that human activity is the direct cause of increased emission of greenhouse gases, and that these increased emissions are happening at a rate that the atmosphere cannot correct on its own. We also have evidence that man-made global warming is creating significant (and perhaps permanent) changes in the Earths climate about which we should be concerned. The increase in the Earths temperature is being caused by the hole in the ozone layer. While it is true that decreasing concentrations of ozone do allow more UV radiation to reach the Earth, this is not the cause of global warming. They are saying that burning fossil fuels causes an increase in greenhouse gases. How can that be when burning uses up the fuel? The combustion process follows the law of conservation of mass which tells us that nothing is ever used up. The two products of combustion are carbon dioxide and water, both of which are greenhouse gases. For an expanded list of misconceptions about climate change see  HYPERLINK "http://www.c2es.org/publications/realities-vs-misconceptions-about-science-climate-change" http://www.c2es.org/publications/realities-vs-misconceptions-about-science-climate-change and  HYPERLINK "http://brynnevans.com/Climate-Change-Part1.pdf" http://brynnevans.com/Climate-Change-Part1.pdf. Anticipating Student Questions (answers to questions students might ask in class) What is the difference between climate change, global warming and the greenhouse effect? According to the EPA: Global warming refers to the recent and ongoing rise in global average temperature near Earth's surface. It is caused mostly by increasing concentrations of greenhouse gases in the atmosphere. Global warming is causing climate patterns to change. However, global warming itself represents only one aspect of climate change. Climate change refers to any significant change in the measures of climate lasting for an extended period of time. In other words, climate change includes major changes in temperature, precipitation, or wind patterns, among other effects, that occur over several decades or longer. ( HYPERLINK "http://www.epa.gov/climatechange/basics/" http://www.epa.gov/climatechange/basics/) How do they decide the temperature of the Earth? Where do they measure it The most obvious problem is that there is not a single temperature for the Earth but an enormous number of local temperatures that span the range from the Antarctic to the tropics and deserts of Africa. In response to this problem, three groups, two in the U.S. and one in Great Britain, analyze thousands of temperature data sets from all over the Earth and determine average temperatures. Other scientists, paleoclimatologists, examine what is called proxy data including ice core samples to determine historical temperatures. How is it possible to keep carbon dioxide gas sequestered undergroundwont it escape to the surface? One example of carbon dioxide sequestering is in Norway where the captured CO2 is injected into sandstone formations under the oceanwhich formerly housed natural gas formations. An 800-foot (250-meter) thick band of sandstoneporous, crumbly rock that traps the gas in the minute spaces between its particlesis covered by a relatively impermeable 650-foot (200-meter) thick layer of shale and mudstone. See More on climate change solutions for additional information on sequestering. How can climate change produce both droughts and floodssometimes in the same region? In general, climatologists are predicting greater extremes in weather due to the changing climate. In addition, if average temperatures increase that could produce two different effects. The first would be more droughts since the higher temperature would mean that water would evaporate more quickly leaving the ground dry. At the same time the warmer atmosphere can hold more water which could translate to more severe storms and heavier rainfall. And both could happen in the same region. What makes some gases greenhouse gases and others not? Its a matter of how bonds are configured in the molecule. If bonds in a molecule allow a temporary dipole to form then the gas may be a greenhouse gas. As the reflected long-wavelength infrared radiation from the Earth strikes the molecule it may cause the bond electrons to shift for just an instant creating a temporary dipole in the molecule and causing the molecule to vibrate excessively. This vibrational motion is the added energy that creates the greenhouse effect in the atmosphere. See More on greenhouse gases above for a little more detail. In-class Activities (lesson ideas, including labs & demonstrations) The University Center for Atmospheric Research (UCAR) has a series of activities that will help students understand various aspects of climate change. Although some are designed primarily for students in grades 6-9, they can easily be adapted for older students. These include The Difference Between Climate and Weather:  HYPERLINK "http://www.ucar.edu/learn/1_2_2_8t.htm" http://www.ucar.edu/learn/1_2_2_8t.htm Climate Variability:  HYPERLINK "http://www.ucar.edu/learn/1_2_2_9t.htm" http://www.ucar.edu/learn/1_2_2_9t.htm Paleoclimates and Pollen:  HYPERLINK "http://spark.ucar.edu/activity/paleoclimates-and-pollen" http://spark.ucar.edu/activity/paleoclimates-and-pollen Time and CyclesDendochronology:  HYPERLINK "http://www.ucar.edu/learn/1_2_2_11t.htm" http://www.ucar.edu/learn/1_2_2_11t.htm What Is a Greenhouse?  HYPERLINK "http://www.ucar.edu/learn/1_3_2_12t.htm" http://www.ucar.edu/learn/1_3_2_12t.htm What Factors Influence a Greenhouse?  HYPERLINK "http://www.ucar.edu/learn/1_3_2_13t.htm" http://www.ucar.edu/learn/1_3_2_13t.htm What Do Concentrations Mean?  HYPERLINK "http://www.ucar.edu/learn/1_4_2_14t.htm" http://www.ucar.edu/learn/1_4_2_14t.htm What Is the Carbon Cycle?  HYPERLINK "http://www.ucar.edu/learn/1_4_2_15t.htm" http://www.ucar.edu/learn/1_4_2_15t.htm Where In the World Is Carbon Dioxide?  HYPERLINK "http://www.ucar.edu/learn/1_4_2_17t.htm" http://www.ucar.edu/learn/1_4_2_17t.htm Human Activity and Climate Change:  HYPERLINK "http://www.ucar.edu/learn/1_4_2_20t.htm" http://www.ucar.edu/learn/1_4_2_20t.htm Students can do lab activities that illustrate reactions that produce or consume carbon dioxide. For example, you can do a demonstration in which you burn a candle in a large beaker. After the candle is lighted, cover the mouth of the beaker with a large watch glass or glass plate. After the candle goes out, remove it carefully and add limewater to the beaker and swirl. The limewater will become cloudy, indicating the presence of CO2 in the beaker as a result of the burning. This activity allows students to plot CO2 data from Mauna Loa, Hawaii, and determine rate of increase:  HYPERLINK "http://serc.carleton.edu/introgeo/interactive/examples/co2.html" http://serc.carleton.edu/introgeo/interactive/examples/co2.html. Lawrence Hall of Science produced a complete teacher guide to climate change science, including numerous activities:  HYPERLINK "http://www.lawrencehallofscience.org/gss/rev/TeacherGuides-PDFs/2CC-TG.pdf" http://www.lawrencehallofscience.org/gss/rev/TeacherGuides-PDFs/2CC-TG.pdf. You can show the formation of acidic water by bubbling carbon dioxide gas through water. Use a pH indicator like bromothymol blue. This is a series of climate change lessons from ARM in Alaska. Most interesting are the lessons later in the unit on the changes in Alaska caused by global warming. ( HYPERLINK "http://education.arm.gov/teacherslounge/lessons/climate_change.pdf?id=89" http://education.arm.gov/teacherslounge/lessons/climate_change.pdf?id=89) The Climate Literacy and Energy Awareness Network (CLEAN) has a series of activities about climate change. The activities include the Earths energy balance, carbon dioxide emissions, paleoclimatology, ice core sampling and energy sources. ( HYPERLINK "http://cleanet.org/clean/educational_resources/index.html" http://cleanet.org/clean/educational_resources/index.html) This guide to climate change is offered by Stanford University and includes several modeling exercises using climate change data. ( HYPERLINK "https://pangea.stanford.edu/programs/outreach/climatechange/curriculum/high-school" https://pangea.stanford.edu/programs/outreach/climatechange/curriculum/high-school) From Google Sites, a listing of 24 exercises and activities about climate change:  HYPERLINK "https://sites.google.com/a/globalsystemsscience.org/courses-lifelines/teaching-resources/activities" https://sites.google.com/a/globalsystemsscience.org/courses-lifelines/teaching-resources/activities. Out-of-class Activities and Projects (student research, class projects) The Intergovernmental Panel on Climate Change (IPCC) has published reports since 1990. In those reports are data that students can analyze for themselves to assess the evidence for global warming and climate change. For example, the 2000 report on greenhouse gas emissions contains a wealth of information about the gases and the ways in which they are emitted. The Intergovernmental Panel on Climate Change issued a 2000 report on greenhouse gas emissions. The report can be accessed here:  HYPERLINK "http://www.ipcc.ch/pdf/special-reports/emissions_scenarios.pdf.Beginning" http://www.ipcc.ch/pdf/special-reports/emissions_scenarios.pdf.Beginning. On page 31 of this report is a series of scenarios and a description of the driving forces for the gas emissions. Students can read these scenarios and report on them in class or in writing. This kind of reading will meet the Common Core Standards for Literacy in Technical Subjects. Students can be assigned sections of this IPCC report on regional impacts of climate change,  HYPERLINK "http://www.ipcc.ch/ipccreports/sres/regional/index.php?idp=173" http://www.ipcc.ch/ipccreports/sres/regional/index.php?idp=173, and asked to prepare 510 slides that summarize the region they researched. Students might create a survey to give to people in their community about climate change and whether people think human activity is one of the causes. Questions can be developed that are based on sound science. The results should be shared with the school and the broader community. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  Becker, R. A Pourable Greenhouse Gas. ChemMatters 2001, 19 (3), pp 1011. The author describes the properties of carbon dioxide. Herlocker, H. Clearing the Air: Treaties to Treatments. ChemMatters 2005, 23 (Special Issue 1), pp 1415. International treaties like the Montreal Protocol and the Kyoto Protocol regarding greenhouse gases and climate change are examined, along with progress towards eliminating the gases. Paradise, A. Air Pollution: What Weather Satellites Tell Us. ChemMatters 2009, 27 (2), pp 1617. This article describes the way in which scientists are using satellite data to inform their knowledge of climate change. Vos, S. Cleaning Up the Air. ChemMatters 2011, 29 (1), pp 1415. The author explains the effect on the atmosphere of the carbon cycle, global warming and greenhouse gases that result from burning fossil fuels like coal. Zajac, L. Kilimanjaro: Peering Through Disappearing Ice. ChemMatters 2011, 29 (1), pp 1618. This article is about using ice core samples, specifically those drilled on the ice sheet at Mt. Kilimanjaro, to develop a record of temperatures in the distant past. This is one of the techniques used to measure temperatures by proxy in centuries prior to temperature records. Web sites for Additional Information (Web-based information sources) More sites on climate change and global warming The most authoritative source on climate change is the Intergovernmental Panel on Climate Change. All of its extensive reports can be accessed here:  HYPERLINK "http://ipcc-wg2.gov/SREX/images/uploads/SREX-All_FINAL.pdf" http://ipcc-wg2.gov/SREX/images/uploads/SREX-All_FINAL.pdf. Refer to the U.S. Environmental Protection Agencys (EPA) site on climate change for basic information:  HYPERLINK "http://www.epa.gov/climatechange/" http://www.epa.gov/climatechange/. NASA also has excellent information on the topic at  HYPERLINK "http://climate.nasa.gov/" http://climate.nasa.gov/. This 2013 article from The Economist provides a current survey of the climate change debate:  HYPERLINK "http://www.economist.com/news/science-and-technology/21574461-climate-may-be-heating-up-less-response-greenhouse-gas-emissions" http://www.economist.com/news/science-and-technology/21574461-climate-may-be-heating-up-less-response-greenhouse-gas-emissions. There are a host of articles about the topic on this New Scientist site:  HYPERLINK "http://www.newscientist.com/topic/climate-change" http://www.newscientist.com/topic/climate-change. The New York Times published a guide to global warming in November, 2013:  HYPERLINK "http://topics.nytimes.com/top/news/science/topics/globalwarming/" http://topics.nytimes.com/top/news/science/topics/globalwarming/. More sites on greenhouse gases The Intergovernmental Panel on Climate Change issued a 2000 report on greenhouse gas emissions. The report can be accessed here:  HYPERLINK "http://www.ipcc.ch/pdf/special-reports/emissions_scenarios.pdf" http://www.ipcc.ch/pdf/special-reports/emissions_scenarios.pdf. This Web page from the American Chemical Society provides a detailed explanation with diagrams of the molecular makeup of greenhouse gases. ( HYPERLINK "http://www.acs.org/content/acs/en/climatescience/greenhousegases/properties.html" http://www.acs.org/content/acs/en/climatescience/greenhousegases/properties.html) This long chapter from a textbook at the University of Chicago offers another explanation of the causes and effects of greenhouse gas behavior. This is an excellent resource. ( HYPERLINK "http://forecast.uchicago.edu/chapter4.pdf" http://forecast.uchicago.edu/chapter4.pdf) The U.S. Environmental Protection Agency predictably has a site with information about climate change and greenhouse gases:  HYPERLINK "http://epa.gov/climatechange/ghgemissions/" http://epa.gov/climatechange/ghgemissions/. From the American Chemical Society comes this very nice Web site on greenhouse gases, including a little history:  HYPERLINK "http://www.acs.org/content/acs/en/climatescience/greenhousegases.html" http://www.acs.org/content/acs/en/climatescience/greenhousegases.html. Elmhurst University published this Web page about greenhouse gases:  HYPERLINK "http://www.elmhurst.edu/~chm/vchembook/globalwarmA5.html" http://www.elmhurst.edu/~chm/vchembook/globalwarmA5.html. This document from the World Meteorological Organization has brief profiles of each of the major greenhouse gases:  HYPERLINK "http://www.wmo.int/pages/prog/arep/gaw/ghg/documents/GHGbulletin_7_en.pdf" http://www.wmo.int/pages/prog/arep/gaw/ghg/documents/GHGbulletin_7_en.pdf. This article summarizes the need to reduce CO2 levels and the evidence for global warming:  HYPERLINK "http://www.csmonitor.com/USA/2009/1125/p02s01-usgn.html" http://www.csmonitor.com/USA/2009/1125/p02s01-usgn.html. More sites on the effects of climate change Climate Change Central lists several effects produced by climate change:  HYPERLINK "http://www.climatecentral.org/?gclid=CI_awJ3nxroCFWrNOgoduyMAwg" http://www.climatecentral.org/?gclid=CI_awJ3nxroCFWrNOgoduyMAwg. NASA also describes present effects and projects future effects:  HYPERLINK "http://climate.nasa.gov/effects" http://climate.nasa.gov/effects. This is one section of an EPA site listed above. On this site are detailed explanations of the effects of climate change:  HYPERLINK "http://www.epa.gov/climatechange/impacts-adaptation/agriculture.html" http://www.epa.gov/climatechange/impacts-adaptation/agriculture.html. More sites on climate change solutions Scientific American magazine lists 10 large-scale ways to mitigate the effects of climate change. The comments are worth scanning as well. ( HYPERLINK "http://www.scientificamerican.com/article.cfm?id=10-solutions-for-climate-change" http://www.scientificamerican.com/article.cfm?id=10-solutions-for-climate-change) This site from the EPA describes for student many of the technologies that can help reduce the effects of climate change:  HYPERLINK "http://www.epa.gov/climatestudents/solutions/index.html" http://www.epa.gov/climatestudents/solutions/index.html. The Union of Concerned Scientists lists several steps to reduce global warming:  HYPERLINK "http://www.ucsusa.org/global_warming/solutions/" http://www.ucsusa.org/global_warming/solutions/. The Intergovernmental Panel on Climate Change issued a 2005 report on carbon dioxide capture and storage. The report can be accessed here:  HYPERLINK "https://docs.google.com/file/d/0B1gFp6Ioo3akWFVURndxRU5xU1E/edit?usp=drive_web&urp=http://www.ipcc.ch/publications_and_data/publicati&pli=1" https://docs.google.com/file/d/0B1gFp6Ioo3akWFVURndxRU5xU1E/edit?usp=drive_web&urp=http://www.ipcc.ch/publications_and_data/publicati&pli=1. Peering through Urine Background Information (teacher information) More on urine The Silveira article states that the range of daily typical urine output for a human can be anywhere from half a liter to two liters. A past ChemMatters Teachers Guide states: A normal 24 hour urine output contains about 60 grams of solid material. About half of this is organic, consisting of substances like urea, uric acid, and creatinine. The inorganic portion will contain substances like sodium chloride, phosphates, sulfates, and ammonia. Normal urine should not contain any glucose or amino acids (ChemMatters Teachers Guide. October 2004, p 22) A seven-year study of human urine was published (The Human Urine Metabolome) in September 2013 in PLOS ONE (See More sites on urine below). Scientists researched and cataloged the entire composition of human urine and gathered the information in an online database. The Huff Post Science article Whats in Pee? Urine Composition Study Reveals More Than 3,000 Chemical Compounds summarizes the research: researchers found that at least 3,079 compounds can be detected in urine. Seventy-two of these compounds are made by bacteria, while 1,453 come from the body itself. Another 2,282 come from diet, drugs, cosmetics or environmental exposure (some compounds belong to more than one group). The complete list of all metabolites that can be detected in human urine using current technologies has been placed into an online public database called the Urine Metabolome Database. The word metabolome refers to the complete collection of metabolites, which are the products of metabolism and include hormones, vitamins and other molecules. The compounds found in human urine fall into 230 different chemical classes. Given that there are only 356 chemical classes in the entire human metabolome, this certainly demonstrates the enormous chemical diversity found in urine, the researchers said. The researchers also found that more than 480 compounds in urine were not previously reported to be in blood, contrary to the long-standing idea that the collection of chemicals in urine is a subset of compounds found in the blood. To find the chemicals in urine, the researchers used a variety of techniques, including nuclear magnetic resonance spectroscopy, gas chromatography, mass spectrometry and liquid chromatography. They analyzed urine samples from 22 healthy people, and scoured more than 100 years of scientific literature about human urine to supplement their findings. (HYPERLINK "http://www.huffingtonpost.com/2013/09/08/what-is-in-pee-urine-chemistry_n_3880868.html"http://www.huffingtonpost.com/2013/09/08/what-is-in-pee-urine-chemistry_n_3880868.html) It can be interesting to contrast the results of this extensive study with information shared over 40 years ago by NASA. Its work on urine and its composition are connected to the development and use of urine purification equipment during space travel. The 1971 NASA report Composition and Concentrative Properties of Human Urine gives an overview and listing of the components of urine known at that time: The composition of human urine has been studied by many investigators and the quantities of 158 different chemical constituents are summarized. These constituents are broadly categorized as electrolytes, nitrogenous compounds, vitamins, hormones, organic acids, and miscellaneous organic compounds. (Putnam, D.F. Composition and Concentrative Properties of Human Urine. National Aeronautics and Space Administration, Washington, DC, July 1971, pp 1, 5; see HYPERLINK "http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044_1971023044.pdf"http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044_1971023044.pdf) Of its extensive list of 158 constituents (although much less extensive than the study reported in 2013), the NASA report lists a subset of the substances in Table 2 of the same document. These 42 selected substances are the components of an analog of human urine synthesized to use in the development and testing of urine purification equipment; the 42 account for over 98 percent of the total solute concentration in urine. (Putnam, D.F. Composition and Concentrative Properties of Human Urine. National Aeronautics and Space Administration, Washington, DC, July 1971, p 5; see HYPERLINK "http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044_1971023044.pdf"http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044_1971023044.pdf) The analog components are broken down into four sections: inorganic salts (8 compounds), urea (1 compoundurea), organic compounds (23 compounds), and organic ammonium salts (10 compounds). The major inorganic salt components are sodium chloride, potassium chloride, and potassium sulfate; major organic compounds are creatinine, uropepsin, creatine, and glycine; major organic ammonium salts are ammonium hippurate, ammonium citrate, and ammonium glucuronate. (Putnam, D.F. Composition and Concentrative Properties of Human Urine. National Aeronautics and Space Administration, Washington, DC, July 1971, p 40; see HYPERLINK "http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044_1971023044.pdf"http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044_1971023044.pdf) Another application that has arisen related to attempting to duplicate the composition of human urine is the sale of synthetic urine products. Web sites selling the product state its For lawful use only, but people do use it to try to outwit drug testing by substituting the synthetic urine for their own. Kits sometimes include tips for how to be sure that the samples temperature is within a typically-accepted testing range (90100 F). Some kits even include a heating pad to bring it to this within-body-temperature range. One might be surprised that the composition of urine can be mimicked to a large extent. The history of the chemistry of urea, one component of urine, is linked to a discovery that was surprising at the timethat what was considered an organic molecule could be synthesized in the laboratory, without a connection to a living being. The discovery is summarized: First discovered in human urine in 1773, it is most notable because of Friedrich Wohlers laboratory synthesis of the compound in 1828. What made this relatively simple synthesis so noteworthy was that prior to that time organic chemicals were considered to be molecules that could only be synthesized by living organisms. It was widely believed that molecules synthesized by a living organism could not be synthesized from their atoms in a laboratory because their synthesis required a vital force that only living things possessed. When Wohler synthesized urea while trying to synthesize ammonium cyanate and then demonstrated that the compound produced could not be distinguished from urea obtained from organic sources, it dealt a great blow to the concept of vital force. (ChemMatters Teachers Guide. October 2004, pp 2223) As mentioned in the Silveira article, urine color and smell can vary. Certain substances that cause these changes in your urine can be related to what you eat and drink. One commonly-mentioned item is asparagus. Research into this phenomenon was reported in the article Excretion and Perception of a Characteristic Odor in Urine after Asparagus Ingestion: a Psychophysical and Genetic Study. The papers introduction states: Some people report that after eating asparagus, their urine has a sulfurous odor like cooked cabbage. For people who smell the odor, they know it to be a result of eating asparagus, whereas others appear to never smell the odor and are surprised to be asked about it. The unusual odor elicited by human urine after asparagus has been mentioned over the years; for instance, Benjamin Franklin noted that a few stems of asparagus eaten shall give our urine a disagreeable odor, and Proust wrote more favorably that asparagus as in a Shakespeare fairy-story transforms my chamber-pot into a flask of perfume. (Pelchat, M.L.; Bykowski, C.; Duke, F.F.; Reed, D.R. Excretion and Perception of a Characteristic Odor in Urine after Asparagus Ingestion: a Psychophysical and Genetic Study. Chem. Senses 2011, 36 (1), p 9; see  HYPERLINK "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002398/" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002398/) The same paper discusses the results of the study, saying that a small percentage of people do not produce the odor, at least not concentrated enough for the odor to be detected. Some people produce an easily-detected odor, some not as much. A small percentage of people are unable to detect the odor. There is also a variation in how sensitive a persons sense of smell is in connection with the particular odor; someone may be less sensitive to the odor, requiring a much higher concentration to be present before they can detect it. For many, urine is something one wishes to simply dispose of as quickly as possible. However, urine can have many useful applications. As mentioned earlier in this section, urine is reclaimed and recycled to produce potable drinking water during space travel. Several applications throughout history are described in the blog post From Gunpowder to Teeth Whitener: The Science Behind Historic Uses of Urine: Prior to the ability to synthesize chemicals in the lab, urine was a quick and rich source of HYPERLINK "http://en.wikipedia.org/wiki/Urea"urea, a nitrogen-based organic compound. When stored for long periods of time, urea decays into HYPERLINK "http://en.wikipedia.org/wiki/Ammonia"ammonia. Ammonia in water acts as a HYPERLINK "http://www.health.ny.gov/environmental/emergency/chemical_terrorism/ammonia_tech.htm"caustic but weak base. Its high pH breaks down organic material, making urine the HYPERLINK "http://www.leathernet.com/tanning.htm"perfect substance for ancients to use in softening and tanning animal hides. Soaking animal skins in urine also made it easier for leather workers to remove hair and bits of flesh from the skin. Even though HYPERLINK "http://soapcauldron.com/soapmakers-journal/soap-history/"early Europeans knew about soap, many launderers preferred to use urine for its ammonia to get tough stains out of cloth. In fact, in ancient Rome, vessels for collecting urine were commonplace on streetspassers-by would relieve themselves into them and when the vats were full their contents were taken to a HYPERLINK "http://penelope.uchicago.edu/Thayer/E/Roman/Texts/secondary/SMIGRA*/Fullo.html"fullonica (a laundry), diluted with water and poured over dirty clothes. A worker would stand in the tub of urine and HYPERLINK "http://voices.yahoo.com/urine-natural-way-clean-clothes-407805.html"stomp on the clothes, similar to modern washing machines agitator. Specific chamberpots HYPERLINK "http://www.objectlessons.org/work-and-innovation-tudors/urine-pots-tudor-replica/s63/a919/"dedicated to urine helped families collect their pee for use as mordants [to be used in for dyeing cloth]. Urine was so important to the textile industry of 16th century England that casks of itan HYPERLINK "http://www.nationaltrust.org.uk/yorkshire-coast/history/view-page/item634280/"estimated amount equivalent to the urine stream of 1000 people for an entire yearwere shipped from across the country to Yorkshire, where it was mixed with HYPERLINK "http://chemistry.about.com/od/moleculescompounds/f/What-Is-Alum.htm"alum to form an even stronger mordant than urine alone. Romans used urine to clean and whiten their teeth, transforming morning breath into a different smell entirely. The active ingredient? You guessed it: ammonia, which lifted stains away. (HYPERLINK "http://blogs.smithsonianmag.com/science/2013/08/from-gunpowder-to-teeth-whitener-the-science-behind-historic-uses-of-urine/"http://blogs.smithsonianmag.com/science/2013/08/from-gunpowder-to-teeth-whitener-the-science-behind-historic-uses-of-urine/) More on urinalysis If youre someone who uses the urine dipsticks described in the Silveira article regularly at home or in connection with your work, you may take this technology for granted. The dipsticks are easy to use and give largely reliable information about an entire list of variables in your body. But, imagine going back in time, before the development of the urine dipstick. Things were not so accurate or so easy. For example, testing for excess sugar in urine to help diagnose diabetes was a more difficult (and less appetizing) proposition. The December 2004 ChemMatters Teachers Guide states: The earliest record [of diabetes] dates back to an Egyptian papyrus of 1552 B.C. Frequent urination is listed as a symptom. Early attempts at diagnosis utilized water tasters, whose function was to taste the urine of individuals who were suspected of having the disease. The excess sugar in their urine gave it a sweet taste. By the early 1800s it was possible to perform chemical tests to detect the presence of sugar in urine. (ChemMatters Teachers Guide. December 2004, p 40) The ChemMatters article Lab on a Stick describes another early test. A major drawback to the tests described above and below is that they could not give a quantitative result, only a yes/no result. Researchers have known for thousands of years that diabetics excrete sugar into their urinea side effect of overwhelming the kidneys with too much blood glucose. So, in one of the first tests for diabetes, doctors poured urine on the ground to see whether it attracted insects. If insects crowded around the puddle, it meant they were attracted to sugar, a dead giveaway for diabetes. Although this test was helpful for determining whether a patient had diabetes, it wasnt sensitive enough to detect how much sugar was present in the urine, an indicator of diabetes severity. (Brownlee, C. Lab on a Stick. ChemMatters 2004, 22 (3), p 10) Beyond the types of substances tested for in the urine dipsticks described in the article such as protein, nitrite, leukocytes, etc., students may be familiar with the use of urine testing for other applications such as drug testing, such as those performed in connection with sporting events, employment, and law enforcement. Olympic competitions in particular involve a vast number of urine samples over a short amount of time using very specific protocols as competitors urine is tested for the presence of substances banned in competition. Such testing must be as accurate and trustworthy as possible, since athletes reputations are on the line, along with medals that can represent the culmination of a career-long effort. In some sporting events, even animal competitors such as horses may be tested for banned substances. A past ChemMatters Teachers Guide summarized the testing procedures used in the 2000 Olympic Games, which involved an estimated 2000 urine samples: An athlete who has been selected to be tested will be notified of his/her selection immediately after his/her event has been completed. From that point on they will be constantly accompanied by a doping control officer until the collection process has been completed. The athlete must provide the sample while under direct observation by a doping control officer of the same gender. It is possible for it to take as much as several hours before some athletes can produce a sample. The doping control officer will do some preliminary testing to insure that the sample is in a suitable state for laboratory testing. This often involves assuring that the sample isnt too dilute. The sample is then divided into two separate samples which are placed in individual uniquely-numbered security containers marked A and B, and all necessary paperwork is completed. The samples are then placed in a transport bag and taken by secure means to the laboratory, which runs the tests and reports back to the IOC Medical Commission. If both samples test positive, the IOC Medical Commission conducts a hearing to determine what recommendations for sanction should be made. During the 1970s and into the 1980s, the effectiveness of drug testing procedures was very open to question. Inadequate technology limited the number of definite and accurate positive tests that could be obtained. Athletes and their coaches learned how to circumvent the system by switching urine samples or by ceasing to take a drug in sufficient time before a competition so that the drug would clear from their system. A large step forward came in 1983, when the introduction of gas chromatography and mass spectroscopy greatly increased the accuracy and sensitivity of drug testing procedures. The strength of this improved technology was dramatically demonstrated in the 1983 Pan American games in Caracas, when numerous athletes tested positive for banned substances and many other athletes left the games without competing, presumably because they knew they would be caught. (ChemMatters Teachers Guide. December 2000, p 9) Urine testing may indicate the specific substances themselves being present in the urine. Depending on the compound, however, the actual substance itself may not be present, but rather one or more metabolites that are formed as the substance is broken down by the body. One example that many people have heard about is that the consumption of poppy seeds, such as in a muffin or bagel, has the potential to lead to a urine test that shows a positive result for the metabolites of the breakdown of heroin, even if the person has not used that drug. The ChemMatters article Seeds of Doubt discusses these cases: A growing body of research indicates that opiates in poppy seeds can cause people to test positive on urine drug tests, even though the drugs arent present in sufficient concentration to have a physiological effect. The presence of opiates in poppy seeds has been known for a long time, but it wasnt relevant until we had urine drug testing, says forensic toxicologist Donna M. Bush, chief of drug testing at the federal Substance Abuse and Mental Health Services Administration. (Goldfarb, B. Seeds of Doubt. ChemMatters 1995, 13 (2), p 4) A colorimetric dipstick that could be dipped in urine to test for 10 different substances at the same time was not the original goal of researchers. At the start, they were looking for a way to more quantitatively indicate the level of sugars in a diabetic patients urine. A history of some of the early work that led up to the development of the urine dipstick is described in the ChemMatters article Lab on a Stick: So, in the early 1900s, researchers developed a method to estimate the level of glucose in urine. Doctors mixed a blue solution of cupric sulfate (CuSO4) into a urine sample, then put in some alkali (strong base) and a complexing agent such as tartrate or ammonia to prevent precipitation of copper(II) hydroxide. Heating the mixture over a Bunsen burner or in a water bath caused any glucose, a strong reducing (electron donating) substance, to react with the blue cupric ions, changing them to copper(I), which precipitates as the orange-brown copper(I) oxide. The extent of the mixtures color changefrom blue to green, brown, and redgave doctors a rough estimate of how much glucose was in a patients blood. The test was colorimetricit relied on a visible color change to track the presence of a chemical. In the 1930s, Walter Compton, the doctor whose family helped found Miles Laboratories, developed an improved version of the same test, with a lot of less mess and effort. He made a tablet with cupric sulfate, sodium hydroxide (the strong base), and citric acid, which he dubbed Clinitest. After putting the tablet in a test tube and adding several drops of water, it fizzed like Alka Seltzer. Heat from the reaction allowed any glucose present to reduce the cupric ions, and doctors compared the remaining mixtures color to a chart to determine the urines glucose level. Clinitest was easy enough for some diabetics to use outside the doctors office, but it still wasnt perfect. Scientists knew that many chemicals, including some drugs, act as reducing substance in urine. So, patients with normal blood glucose levels frequently ended up with false positive results for diabetes. To weed out these bogus results, Helen and Al Free, along with other chemists at Miles Laboratories, developed a tablet test for ketone bodies, a byproduct in diabetics urine caused by metabolizing fat instead of glucose. The white tablet contained alkali and nitroprusside, [Fe(CN)5(NO)]2-. If a drop of urine turned the tablet purple, the patient had diabetes. (Brownlee, C. Lab on a Stick. ChemMatters 2004, 22 (3), p 10) The names Helen and Al Free in the last paragraph are the ones to remember in connection with the urine dipstick. They played a huge role in developing a new glucose test, realizing that it could be incorporated into a paper strip, and then combining it with other relevant tests. The article continues with further developments: For years, doctors had to perform both tests and a blood test to get an accurate reading of a patients blood sugar. But in 1953, diabetes diagnostics took a giant leap ahead. A factory owned by Miles Laboratories developed an enzyme called glucose oxidase, which reacted only with glucose. Al Free immediately noticed the potential for a brand new type of glucose test. When glucose oxidase reacts with glucose, it forms two products, gluconic acid and hydrogen peroxide. Testing for gluconic acid proved too tricky for easy analysis, so the Miles chemists focused on a reaction to show the presence of hydrogen peroxide instead. The researchers added peroxidase to react with hydrogen peroxide, as well as a benzidine, a type of chromogen, or chemical that changes color when it becomes oxidized. The reaction worked like a charm, turning shades of blue with different glucose levels. But the test was still too complicated for most diabetics to use at home. After doing thousands of tests on spot plates and in test tubes, Al had an ideaif the same reagents were on a piece of paper, could you dip it into a urine sample and get the same results? After many more tests, the researchers found that the answer was yes. But the Frees and a hundred other researchers at the Miles Ames Research Laboratory couldnt stop quite yet. They developed a colorimetric paper test for albumin, a plasma protein that leaks into diabetics urine when their kidneys fail. Since doctors frequently test for glucose and albumin at the same time, they decided to put the two tests on the same paper strip. They later incorporated the ketone test and added colorimetric analyses for bilirubin and urobilinogen, byproducts formed by the breakdown of red blood cells and good indicators for liver failure. Later came tests on the same strip for occult (hidden) blood and proteintwo signs of kidney damageas well as leukocytes and nitrite, signs of a urinary tract infection. The researchers rounded off the strips with reagents for pH and specific gravity, a measure of concentration. The test strips were so easy to use that they became an instant hit and a big seller for the Ames division of Miles Laboratory (later to become Bayer). (Brownlee, C. Lab on a Stick. ChemMatters 2004, 22 (3), pp 1011) Whats in the future for urinalysis? Various reports within the past few years suggest that urinalysis may be used to help detect an ever-expanding list of conditions. These include several types of cancer, such as prostate, bladder, and gastroesophageal. Currently, using urine to detect these conditions is not as easy as dipping a test strip. For example, in a study related to gastroesophageal cancer detection, researchers collected urine samples, placed them in sealed containers, then inserted a hypodermic needle into the space above the urine sample where any volatile gaseous molecules would collect. The needle was attached to a tube connected to a mass spectrometer, which then analyzed any compounds present in the gas. The ability to more easily test for this particular type of cancer in order to catch the disease in its early stages would be helpful. Only 20% of people with cancers of the stomach or esophagus receive treatment because the diagnosis often comes too late for doctors to stop the cancer. Also patients dont usually experience symptoms until the cancer is advanced. (Gebel, E. Mass Spectrometry Detects Cancer Biomarkers in the Chemical Cloud Hovering over Urine Samples. C & E News, March 13, 2013; see HYPERLINK "http://cen.acs.org/articles/91/web/2013/03/Mass-Spectrometry-Detects-Cancer-Biomarkers.html"http://cen.acs.org/articles/91/web/2013/03/Mass-Spectrometry-Detects-Cancer-Biomarkers.html) Even conditions that one might not normally think of being linked to urine hold promise for testing. One example is the eye disease retinitis pigmentosa, which can cause blindness. Urine samples can be profiled for different species of organic compounds known as dolichols. (HYPERLINK "http://today.duke.edu/2013/10/eyetest"http://today.duke.edu/2013/10/eyetest) MIT researchers have reported in 2013 on the creation of a urine test that uses nanoparticles to help indicate the level of blood clotting that could be present in the person being tested. One of the researchers, Prof. Sangeeta Bhatia, describes two situations where such a test could be useful: For screening patients in the emergency room who complain of symptoms indicative of a blood clot, and to monitor patients at high risk, for example people who fly frequently or who spend a lot of time recovering from surgery in bed. (HYPERLINK "http://www.medicalnewstoday.com/articles/267640.php"http://www.medicalnewstoday.com/articles/267640.php) Connections to Chemistry Concepts (for correlation to course curriculum) Organic vs. InorganicThe articles initial description of urine states that it includes organic solutes and inorganic ions. Students could research the formulas and structures of the organic solutes mentioned in the article to contrast with the inorganic ions discussed. ConcentrationThe amount of a persons fluid intake affects the concentration of his or her urine, which then affects its color. This could be linked to a larger discussion of how concentration is treated in relation to chemistry, typical units used, etc. Physical vs. Chemical PropertiesThe articles section on analyzing urine discusses observations of both the physical properties and chemical composition of urine. Properties of urine could be separated into the categories of physical or chemical. Chemical IndicatorsDipsticks used to test urine are coated with various indicators that allow the user to compare the color result on the dipstick to the range of colors on the dipstick container. Students could investigate the various indicators used on the dipsticks. EnzymesTwo of the reactions on the dipstick portion that indicates the amount of glucose present in urine involve the enzymes glucose oxidase and peroxidase. The action of these enzymes could be discussed and compared with other enzymes. Possible Student Misconceptions (to aid teacher in addressing misconceptions) A urine test can tell you everything you need to know with results that are 100% accurate all of the time. Urine tests can be used to detect many, but not all substances in urine. There is also a limit to how small a concentration of a substance in your urine a test can detect. In addition, if a urine test such as a dipstick diagnoses a problem, further tests are needed, because there is still a chance that the dipstick results show something abnormal when everything is normal, known as a false positive. Anticipating Student Questions (answers to questions students might ask in class) How does a urine pregnancy test work? Pregnancy tests rely on the presence of the hormone human chorionic gonadotropin (hCG), a glycoprotein that is secreted by the placenta shortly after fertilization. The tests work by binding the hCG hormone, from either blood or urine, to an antibody and an indicator. The antibody will only bind to hCG; other hormones will not give a positive test result. The usual indicator is a pigment molecule, present in a line across a home pregnancy urine test. ( HYPERLINK "http://chemistry.about.com/od/chemistryfaqs/f/pregnancytest.htm" http://chemistry.about.com/od/chemistryfaqs/f/pregnancytest.htm) I saw on a TV show that after someone got stung by a jellyfish, someone urinated on their leg to make it stop hurting. Does this work? No. When someone is stung by a jellyfish, structures from the jellyfish that produce venom can remain in the skin. It is best not to disturb the balance of solutes within these structures, since they can release more venom. It is recommended to use a saltwater rinse rather than a freshwater rinse or urine to help maintain this balance. ( HYPERLINK "http://www.scientificamerican.com/article.cfm?id=fact-or-fiction-urinating" http://www.scientificamerican.com/article.cfm?id=fact-or-fiction-urinating) Why do people drink cranberry juice to help prevent a urinary tract infection? What does it do to the urine? The thinking behind the use of cranberry juice to help prevent urinary tract infections was that it made the urine more acidic, so bacteria that caused the infection would be less likely to be able to grow. However, researchers now believe that cranberry juice may make it more difficult for the bacteria to adhere to the urinary tract walls. There are several pros and cons to using cranberry juice or cranberry tablets in this preventive way. ( HYPERLINK "http://www.webmd.com/urinary-incontinence-oab/womens-guide/cranberries-for-uti-protection" http://www.webmd.com/urinary-incontinence-oab/womens-guide/cranberries-for-uti-protection) What is a kidney stone? Kidney stones are small, hard deposits that form inside your kidneys. The stones are made of mineral and acid salts. Kidney stones have many causes and can affect any part of your urinary tract from your kidneys to your bladder. Often, stones form when the urine becomes concentrated, allowing minerals to crystallize and stick together. Passing kidney stones can be quite painful, but the stones usually cause no permanent damage. ( HYPERLINK "http://www.mayoclinic.com/health/kidney-stones/DS00282" http://www.mayoclinic.com/health/kidney-stones/DS00282) In-class Activities (lesson ideas, including labs & demonstrations) The 2004 issue of the American Chemical Societys publication Celebrating Chemistry focuses on health and wellness and includes the hands-on activity Urine the Know. Students test four solutions (distilled water, water with powdered milk, pediatric electrolyte solution, pediatric electrolyte solution with powdered milk) using two types of urinalysis dipsticks. The results reveal whether there is glucose and/or protein present in the samples. While designed for students younger than high school level, this activity can provide a jumping off point for exploring the use of urinalysis dipsticks or could be a hands-on activity to use for outreach with elementary level students. ( HYPERLINK "http://www.acs.org/content/dam/acsorg/education/outreach/2004-ncw-fall-edition-health-and-wellness.pdf" http://www.acs.org/content/dam/acsorg/education/outreach/2004-ncw-fall-edition-health-and-wellness.pdf) Teachers could invite people who use urinalysis in connection with their jobs, such as lab technicians, law enforcement officers, and companies that perform medical screening and testing. An obstetrician (OB) or nurses who work with an OB could discuss urinalysis tests that are specifically used with pregnant women, such as pregnancy tests, and urinalysis for protein, sugars, bacteria, etc. The 2001 issue of ChemMatters includes a hands-on experiment related to kidney dialysis. Students prepare a cornstarch slurry, place it in a zip-seal plastic bag, and place the bag in an iodine solution. They observe any movement of molecules, make a conclusion about the relative sizes of starch and iodine molecules, and relate the model to the process of kidney dialysis. (Thielk, D. Kidney DialysisA Working Model You Can Make. ChemMatters 2001, 19 (2), p 12) A handout for a urinalysis activity at a community college gives links for students to do initial research on substances tested for with dipstick urine tests, then has students use the dipsticks with synthetic urine samples. ( HYPERLINK "http://faculty.spokanefalls.edu/InetShare/AutoWebs/adrianab/Urinalysis.docx" http://faculty.spokanefalls.edu/InetShare/AutoWebs/adrianab/Urinalysis.docx) Out-of-class Activities and Projects (student research, class projects) Students could research the chemistry behind various drug testing, for example, those done as a condition of employment or in connection with law enforcement. They could then research (through the internet, interviews, etc.) potential ways that people have tried to fool these tests and whether they are successful methods. Students could take a field trip to a medical testing facility that performs urine testing to learn about their testing protocols and the different types of urinalysis they perform. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  The ChemMatters article Seeds of Doubt discusses the experience of a woman whose urine drug test done for employment purposes indicated she may have taken heroin, but was instead due to eating a poppy seed bagel. (Goldfarb, B. Seeds of Doubt. ChemMatters 1995, 13 (2), pp 46) The ChemMatters article Drug Detection at the OlympicsA Team Effort describes testing athletes for prohibited substances at the 2000 Summer Olympic Games. (Morton, R. Drug Detection at the OlympicsA Team Effort. ChemMatters 2000, 18 (4), pp 79) The ChemMatters article Urine: Your Own Chemistry discusses the composition of urine, how it is produced, and urinalysis. (Kimbrough, D.R. Urine: Your Own Chemistry. ChemMatters 2002, 20 (3), pp 1415) The ChemMatters article Lab on a Stick presents the history of the development of the urinalysis dipstick, including an interview with one of the main researchers, Helen Free. (Brownlee, C. Lab on a Stick. ChemMatters 2004, 22 (3), pp 912) The ChemMatters article Kidney DialysisThe Living Connection outlines the work done by the kidneys to rid the body of toxins and how dialysis treatments attempt to replicate this filtration process. (Thielk, D. Kidney DialysisThe Living Connection. ChemMatters 2001, 19 (2), pp 1012) Web sites for Additional Information (Web-based information sources) More sites on urine The blog post New Law of Urination: Mammals Take 20 Seconds to Pee on the National Geographic Web site discusses the similarity between how long it takes various mammals to urinate, even though the volumes of urine are dramatically different. ( HYPERLINK "http://newswatch.nationalgeographic.com/2013/10/23/new-law-of-urination-mammals-take-21-seconds-to-pee/" http://newswatch.nationalgeographic.com/2013/10/23/new-law-of-urination-mammals-take-21-seconds-to-pee/) A newspaper article discusses the use of synthetic urine kits to attempt to avoid positive results for drugs in urine testing. ( HYPERLINK "http://www.usatoday.com/story/news/nation/2013/07/08/fake-urine-poses-law-enforcement-challenge/2498017/" http://www.usatoday.com/story/news/nation/2013/07/08/fake-urine-poses-law-enforcement-challenge/2498017/) A list of different potential causes of a color change of urine are summarized. ( HYPERLINK "http://www.mayoclinic.com/health/urine-color/DS01026/DSECTION=causes" http://www.mayoclinic.com/health/urine-color/DS01026/DSECTION=causes) A University of Delaware professor summarizes various research on the burning questions of whether all humans produce a distinctive odor in their urine after eating asparagus and whether all humans can detect the odor. ( HYPERLINK "http://udel.edu/~mcdonald/mythasparagusurine.html" http://udel.edu/~mcdonald/mythasparagusurine.html) A brief piece reports on a mobile phone battery that can be charged by microbial fuel cells that use urine. (HYPERLINK "http://www.rsc.org/chemistryworld/2013/07/urine-microbial-fuel-cell-mobile-phone"http://www.rsc.org/chemistryworld/2013/07/urine-microbial-fuel-cell-mobile-phone) The article The Human Urine Metabolome reports on an extensive study into the components of human urine. It includes a link to an online database containing information on the components. ( HYPERLINK "http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073076" \l "pone-0073076-t001" http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073076 - pone-0073076-t001) Basic drawings and animations on this Web site outline the function of the kidneys and their production of urine. ( HYPERLINK "http://www.biologymad.com/resources/kidney.swf" http://www.biologymad.com/resources/kidney.swf) More sites on urinalysis An American Chemical Society National Historic Chemical Landmark has been established at the ETHOS Science Center (in Elkhart, Indiana, to honor the development of diagnostic dipsticks for urine samples. The site includes background information on its history. ( HYPERLINK "http://www.acs.org/content/acs/en/education/whatischemistry/landmarks/diagnosticteststrips.html" http://www.acs.org/content/acs/en/education/whatischemistry/landmarks/diagnosticteststrips.html) A March 13, 2013, Chemical & Engineering News article reports on the possibility of using the volatile compounds that a urine sample gives off to detect cancer. ( HYPERLINK "http://cen.acs.org/articles/91/web/2013/03/Mass-Spectrometry-Detects-Cancer-Biomarkers.html" http://cen.acs.org/articles/91/web/2013/03/Mass-Spectrometry-Detects-Cancer-Biomarkers.html) A urine test uses nanoparticles as a detector for blood clots. ( HYPERLINK "http://www.medicalnewstoday.com/articles/267640.php" http://www.medicalnewstoday.com/articles/267640.php) Although this YouTube video is essentially an advertisement aimed at medical providers for a particular brand of urinalysis dipsticks, it provides an interesting look at the features and selling points of the dipsticks. ( HYPERLINK "http://www.youtube.com/watch?v=FbU_boC-Xws" http://www.youtube.com/watch?v=FbU_boC-Xws) More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers) A urinalysis lesson plan is offered by the Michigan Association of Laboratory Science Educators. It includes a laboratory exercise where students perform urinalysis using dipsticks with different fake urine samples to diagnose simulated patients. ( HYPERLINK "http://medlabcareers.msu.edu/toolkit web/toolkit_urin.html" http://medlabcareers.msu.edu/toolkit web/toolkit_urin.html) Section 11 of the document Forensic Sciences: A Crime Scene Investigation Unit focuses on urine analysis related to forensics. It includes an activity performed with simulated urine. ( HYPERLINK "http://www.edu.gov.mb.ca/k12/cur/science/found/c_topics30s/forensics_unit.pdf" http://www.edu.gov.mb.ca/k12/cur/science/found/c_topics30s/forensics_unit.pdf) Morphine & Heroin: The Yin and Yang of Narcotics Background Information (teacher information) More on the physiological effects of morphine Morphine and the opiates are probably some of the most valuable drugs in medicine. Their ability to alleviate pain and suffering led Thomas Sydenham, an English doctor in the nineteenth century to call them Gods own medicine. As noted in the ChemMatters article, there is a long history involving the use of opiates for a variety of reasons including for religious purposes. For the scientific community, it is of interest to know how these opiates work in the nervous system in order to not only make judicious and effective use of the drugs but to also develop alternatives that may not have as many side effects and/or addicting qualities. There are other drugs that relieve pain but are non-narcotic and are labeled as analgesics, the most familiar being aspirin. The specifics as to how the opiates work in the nervous system involve several chemicals endogenous to the central and peripheral nervous systems. These chemicals include naturally occurring opiate-like compounds known as endorphins, which is a word that blends two descriptive termsendogenous (meaning developing within) and morphine. In addition, several other chemicals, through their migration and attachment to special reactor or action sites, control the responses of nerve cells. They include dopamine and glutamine, which are protein and amino acid molecules with specific shapes that are part of the functioning of a nerve impulse. Dopamine, endorphins and opioids, such as morphine, affect specific areas of the brain to moderate sensations of pain generated by the nervous system. Dopamine is both a hormone and a neurotransmitter. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol) and its molecular structure is shown below as:  In the brain, it acts as a neurotransmitter and is produced in a region of the brain known as the substantia nigra.  The diagram above [right] shows the major steps in the HYPERLINK "http://thebrain.mcgill.ca/flash/a/a_01/a_01_m/a_01_m_fon/a_01_m_fon.html" \l "2" action of the neurotransmitter dopamine. First, dopamine is synthesized from the amino acid tyrosine. The dopamine is then stored in the synaptic vesicles of the presynaptic neuron until it receives HYPERLINK "http://thebrain.mcgill.ca/flash/a/a_01/a_01_cl/a_01_cl_fon/a_01_cl_fon.html" action potentialsthat cause it to release the dopamine into the synaptic gap by a process called exocytosis. On the post-synaptic neuron, the dopamine then binds to specific receptors. The dopamine is subsequently reabsorbed by transporters on the terminal button of the dopaminergic presynaptic neuron. There the dopamine is either stored again in vesicles or broken down by a mitochondrial enzyme called monoamine oxidase. (source:  HYPERLINK "http://thebrain.mcgill.ca/flash/i/i_03/i_03_m/i_03_m_que/i_03_m_que.html" http://thebrain.mcgill.ca/flash/i/i_03/i_03_m/i_03_m_que/i_03_m_que.html ) Loss of dopamine neurons in this area of the brain is the cause of Parkinsons disease, in which people lose their ability to execute smooth, controlled movements. Dopamine has many other functions in the brain including important roles in behavior and cognition, motor activity, motivation and reward (pleasure sensations), and regulation of milk production. (For this function, dopamine is secreted by the hypothalamus portion of the brain). In the frontal lobes of the brain, dopamine assists in controlling the flow of information from other areas of the brain. Dopamine disorders in the frontal lobes can cause a decline in neurocognitive function including memory, attention, and problem solving. On the other hand, intake of various drugs including opiates, nicotine, and marijuana, increase the levels of dopamine secreted which induces a feeling of euphoria. Morphine and other opiates bind to the same neural sites as the endorphins and enkephalins, naturally occurring peptides that block pain like endorphins. In so doing, the morphine molecules keep the ion channels (primarily Cl1-) open and also block the reabsorption of the dopamine into the neurons, prolonging the sensation of euphoria. The reason that opiates such as heroin and morphine affect us so powerfully is that these exogenous substances bind to the same receptors as our endogenous opioids (endorphins and enkephalins. There are three kinds of receptors widely distributed throughout the brainmu, delta, and kappa receptors. These receptors, through second messengers, also influence the likelihood that ion channels (primarily chloride ion) will open, which in certain cases reduces the excitability of neurons. This reduced excitability is the likely source of the euphoric effect of opiates and appears to be mediated by the mu and delta receptors. This euphoric effect also appears to involve another mechanism in which the GABA-inhibitory interneurons of the ventral tegmental area come into play. By attaching to their mu receptors, exogenous opioids reduce the amount of GABA released (see animation, referenced below). Normally, GABA reduces the amount of dopamine released in the nucleus accumbens, a section of the brain at the bottom of the frontal lobe region. (See a picture of the brain with nucleus accumbens labeled in the More on chemically interrupting dependency section that follows.) By inhibiting this inhibitor, the opiates ultimately increase the amount of dopamine produced and the amount of pleasure felt. Cocaine acts by blocking the reuptake of certain neurotransmitters such as dopamine, norepinephrine, and serotonin. By binding to the transporters that normally remove the excess of these neurotransmitters from the synaptic gap, cocaine prevents them from being reabsorbed by the neurons that released them and thus increases their concentration in the synapses (see animation). As a result, the natural effect of dopamine on the post-synaptic neurons is amplified. The group of neurons thus modified produces much more dependency (from dopamine), feelings of confidence (from serotonin), and energy (from norepinephrine) typically experienced by people who take cocaine. A description and animation of dopamine action on a neuron is found at  HYPERLINK "http://www.addictionscience.net/ASNbiological.htm" http://www.addictionscience.net/ASNbiological.htm. Also, an animation for the effect of various drugs on the neuron activity involving GABA (gamma amino butyric acid), the chloride ion (Cl1-) channels, and dopamine secretion and reabsorption is found at this site:  HYPERLINK "http://thebrain.mcgill.ca/flash/i/i_03/i_03_m/i_03_m_par/i_03_m_par_heroine.html#drogues" http://thebrain.mcgill.ca/flash/i/i_03/i_03_m/i_03_m_par/i_03_m_par_heroine.html#drogues. Click on the without heroin or with heroin buttons at the top of the diagram. More on the sensation of pain In 1965, a theory about pain was proposed by Canadian psychologist Ronald Melzack and British neurobiologist Patrick Wall that suggested that nerve cells in the spinal cord act like gates, opening to allow pain messages to pass or closing to block pain messages from travelling to the brain. The input to the spinal cord is dependent on two different types of nervesone type for pain and another for touch and pressure. Since the publication of the gate control theory, scientists have elucidated more clearly what it is that sends the pain message to the brain or doesnt. They now know, for instance, that neurotransmitters (chemical messengers found naturally in the brain and spinal cord) are important in conducting signals from one nerve cell tothe next. Neurotransmitters stored in the bulbous end of a nerve cell travel across a junction (synapse) to attach to receptors on the surface of a neighboring cell and thereby either prompt or inhibit a continued electrical impulse along the nerve. Gamma-amino butyric acid (GABA), a naturally occurring amino acid, is an example of an inhibitory neurotransmitter that prevents nerve cells from firing, thus diminishing the sensation of pain. On the other hand, the neuropeptide (an organic compound composed of amino acids in a defined order) known as Substance P. is a neurotransmitter that increases the conduction of the pain stimulus to the brain. Substance P. is released in response to noxious stimuli or injury to tissues, and acts like a spark to speed the pain impulse along the nerves. Endorphins and enkephalins bind with the nerve cell receptors required to send the electrical impulse across the synapse and thus, by closing the pain gates, block the release of neurotransmitters responsible for increasing pain perception. Research has shown that certain behavioral habits, like regular exercise or positive thinking, can increase levels of endorphins and enkephalins. (from Harvard Magazine,  HYPERLINK "http://harvardmagazine.com/2005/11/the-science-of-hurt.html#gates" http://harvardmagazine.com/2005/11/the-science-of-hurt.html#gates and a related second Harvard article,  HYPERLINK "http://harvardmagazine.com/2005/11/relieving-pain.html" http://harvardmagazine.com/2005/11/relieving-pain.html ) More on controlling pain Management of pain is more than just ingesting or injecting an opioid-based medication. First there is consideration of the source of the pain. Since the 1960s, a more scientific approach has revealed a number of factors that are part of pain mitigation protocols. A fundamental principle is that the peripheral nervous system has a gate system in which two types of nerve input compete to breach the gate (nerve cells opening and closing) in the spinal cord. As mentioned above, one input is from pain itself, and the other is from touch and pressure. There is a balance between these two inputs that determines if the gate opens, sending signals to the brain. This balance explains why counter stimulation works. Rubbing a stubbed toe works to counter the pain. Acupuncture as well as the application of heat and cold also can counter the pain sensation (nerve impulse). The other side of pain has to do with psychological factors. An amputee sometimes still feels pain in a missing limb. And individuals with pain from such conditions as shingles, arthritis, aftereffects of abdominal surgery, have increased sensitivities to pain similar to turning up the volume on a radio. Mentally, the psyche becomes hypersensitive to pain. What that suggests is that treatment of pain may well involve treating the emotional circuitry as well as the pain transmitting circuitry. Doing surgery requires not only anesthetics but also analgesics post-operatively. You need to block the nerve pathways that transmit pain from its source as well as blocking the pain message in specific areas of the brain. There are a number of modalities that need to be considered when treating pain. Pharmacological choices include non-steroidal anti-inflammatory drugs (NSAID) such as aspirin, ibuprofen, and acetaminophen. Steroids themselves as well as anticonvulsants (working to counter the pain inputs to the brain as with rubbing the banged up toe!) also may be prescribed. Stimulation-induced analgesia is an interesting device, consisting of a small battery-powered device that provides low voltage electricity applied through electrodes placed under the skin. This electrical stimulation mimics the touch and pressure inputs to the spinal cord nerve cells, countering the pain impulses trying to get through the gate mentioned earlier. This method is known as transcutaneous electrical nerve stimulation (TENS). The interesting thing is that in ancient Egypt, people treated pain with electricity coming from electrical catfish which they held in their hands to get the electrical stimulation! Acupuncture works the same way as a competing stimulus to the pain-generating nerve impulses. Anti-depressants can be used to increase the supply of a neurotransmitter, serotonin, that helps activate the bodys natural pain-relief system. This seems to be the drug of choice for treating the pain associated with shingles. Behavior/Psychological intervention (therapy) Surgical intervention such as in the case of herniated intervertebral disks, removing part of the damaged disk. But this is normally done only after more conservative interventions have failed to relieve the pain. Physical measures include regular exercise for muscle tone, strength and flexibility, physical therapy, and massage. (source:  HYPERLINK "http://harvardmagazine.com/2005/11/relieving-pain.html" http://harvardmagazine.com/2005/11/relieving-pain.html ) More on alkaloids A summary of the basics about alkaloids follows. 1. Contains nitrogen - usually derived from an amino acid. 2. Bitter tasting, generally white solids (exception - nicotine is a brown liquid). 3. They give a precipitate with heavy metal iodides. Most alkaloids are precipitated from neutral or slightly acidic solution by Mayer's reagent (potassiomercuric iodide solution). Cream coloured precipitate. Dragendorff's reagent (solution of potassium bismuth iodide) gives orange coloured precipitate with alkaloids. Caffeine, a purine derivative, does not precipitate like most alkaloids. 4. Alkaloids are basic - they form water soluble salts. Most alkaloids are well-defined crystalline substances which unite with acids to form salts. In plants, they may exist in the free state, as salts or as N-oxides. 5. Occur in a limited number of plants. Nucleic acid exists in all plants, whereas, morphine exists in only one plant species. (from  HYPERLINK "http://www.friedli.com/herbs/phytochem/alkaloids/alkaloid1.html" http://www.friedli.com/herbs/phytochem/alkaloids/alkaloid1.html ) More details about alkaloids: Alkaloid, a chemical substance of plant origin composed of carbon, hydrogen, nitrogen, and (usually) oxygen. The alkaloids are organic bases similar to the alkalis (inorganic bases); the name means alkali-like. Most alkaloids have pronounced effects on the nervous system of humans and other animals. Many are used as drugs. Some familiar alkaloids are caffeine, nicotine, quinine, cocaine, and morphine. Alkaloids occur mainly in various genera of seed plants, such as the opium poppy and tobacco plant. Alkaloids can be found in almost all parts of these plants, including the leaves, roots, seeds, and bark. Each plant part usually contains several chemically related alkaloids. The function of alkaloids in plant metabolism is not known. Of the hundreds of alkaloids found in nature, only about 30 are used commercially. Alkaloids must be extracted from plants before they can be used. After the plants have been dried and crushed, chemical reagents such as alcohol and dilute acids are used to extract the alkaloid content from the plant material. Pure alkaloid extracts are usually bitter, colorless solids. Some alkaloids, such as reserpine and morphine, are synthesized (produced artificially). Uses of Alkaloids Some alkaloids, such as nicotine, are used in pesticides, and others are used as chemical reagents. The primary use of alkaloids, however, is in medicine, because they can act quickly on specific areas of the nervous system. Alkaloids are the active components of many anesthetics, sedatives, stimulants, relaxants, and tranquilizers. They are taken by mouth and administered by injection. Except under a physician's supervision, use of alkaloids is dangerous, because most are habit-forming (for example, almost all narcotics are alkaloids) and large doses can be poisonous. Strychnine, used in small doses as a stimulant and a tonic, is highly poisonous. Quinine, used in treating malaria, can cause dizziness if taken in large doses. Morphine and cocaine are among the most effective drugs known for temporarily relieving pain without causing loss of consciousness. However, these two alkaloids are habit-forming and can be harmful if their use is continued. Curare, used as a muscle-relaxing drug and in arrow poisons used by South American Indians, is a mixture of various alkaloids. Alkaloid Substitutes In most cases, the extraction of natural alkaloids and the synthesis of alkaloids are complicated, costly processes. Furthermore, alkaloid drugs usually produce unpleasant side effects. For these reasons, several synthetic compounds have been developed for use as alkaloid substitutes. For example, Novocain (a trade name for procaine) is often used instead of cocaine, and Demerol (a trade name for meperidine) is often substituted for morphine. Alkaloid substitutes are usually less toxic than alkaloids, but are also generally less potent. ( HYPERLINK "http://science.howstuffworks.com/alkaloid-info.htm" http://science.howstuffworks.com/alkaloid-info.htm) More on the history of heroin A timeline for the recent history of heroin is as follows: 1853 Hypodermic needle-syringes with a point fine enough to pierce the skin are invented simultaneously by Charles Gabriel Pravaz (French surgeon) and Alexander Wood (Scottish physician). It is first used to inject morphine intravenously. 1874 Heroin is first synthesized from Morphine by chemist C.R. Alder Wright at St. Mary's Hospital in London. Its potential was not recognized. 1897 Heroin is synthesized by Felix Hoffman at Bayer Pharmaceutical. Bayer immediately recognized its potential and began marketing it heavily for the treatment of a variety of respiratory ailments. 1898 One year after beginning sales, Bayer exports heroin to 23 countries. Early 1900s Doctors and pharmacists begin noticing that patients are consuming large amounts of heroin containing cough remedies. 1906 Pure Food and Drug Act is passed, regulating the labelling of products containing Alcohol, Opiates, Cocaine, and Cannabis, among others. The law went into effect Jan 1, 1907 1911 British Pharmaceutical Codex notes that heroin is as addictive as morphine. 1913 Bayer ceases producing heroin. Dec 17, 1914 The Harrison Narcotics Tax Act is passed, regulating and imposing a tax upon the sale of Opium, Heroin and Cocaine for the first time. The Act took effect Mar 1, 1915. 1924 The Heroin Act passes, making manufacture and possession of heroin illegal in the U.S. 1965-1970 U.S. involvement in Vietnam is blamed for the surge in illegal heroin being smuggled into the States. 1971 10-15% of American servicemen in Vietnam are addicted to heroin. (from  HYPERLINK "http://www.erowid.org/chemicals/heroin/heroin_timeline.php" http://www.erowid.org/chemicals/heroin/heroin_timeline.php) More on chemically interrupting drug dependency  Neuroscientists have known for some time that marijuanaalong with many other drugs with abuse potential, including nicotine and opiatesinduces a feeling of euphoria by increasing levels of dopamine in the brain. In recent times, Robert Schwarcz (Univ. of Maryland) and others have also discovered that kynurenic acid is crucially involved in the regulation of brain activity driven by dopamine. (effect is in the nucleus accumbens region of the brain) Knowing the role of kynurenic acid, which has a similar molecular structure to dopamine, Schwarcz has found that increasing the levels of the acid interferes with the euphoric effects of various opiates as well as marijuana and nicotine by decreasing the activity level of the dopamine neurotransmitter which is associated with pleasure. The question now is whether investigators can find a safe way to administer the kynurenic acid because there is a need to have the correct level (homeostasis) of dopamine through kynurenic acid control.   (dopamine) (kynurenic acid) Connections to Chemistry Concepts (for correlation to course curriculum) OrganicThis is a very large category of molecules, often derived from natural sources, that are built around carbon, with its four bonding positions, creating very large molecules such as proteins that permeate the biological world. Important organic chemicals of the nervous system, associated with pain sensation and drug addiction, include neurotransmitters such as dopamine, serotonin, and epinephrine, among others. AlkaloidThis special category of organic molecule from plant sources includes such well known chemicals as caffeine, nicotine, quinine and as morphine. The molecules are composed of carbon, hydrogen, nitrogen and usually oxygen in multiple ring systems. Many are derived from amino acids (amine), hence the names of the various alkaloids often end in -ine. Alkaloids are extracted by dissolving the plant-source material in acid. OpioidAn opioid, as a category of chemical compound, is any psychoactive chemical that resembles morphine or other opiates in its pharmacological effects. One of the main functions of opioids is to produce sedation and pain relief through those parts of the brain that control emotion. Organic reactionsacetylationThis category of chemical reaction is called ethanoylation in the IUPAC nomenclature. The reaction introduces an acetyl functional group into a molecule containing a hydroxyl group, replacing the hydrogen atom with the acetyl group (-CH3CO), producing a specific ester, the acetate. This particular reaction is important in the formation of proteins and in the regulation of deoxyribonucleic acid (DNA). SolubilityHeroin has a lower solubility in water than does morphine, due to the acetylation of heroin. Lower polarity of the heroin molecule explains this. Its lower solubility in water also means a greater solubility in fats and oils and helps explain why heroin can more easily cross the blood-brain barrier. Possible Student Misconceptions (to aid teacher in addressing misconceptions) Eating lots of poppy seeds (on a bagel or in a poppy seed cake) can produce the same effect as taking opium or heroin. Although poppy seeds are from the plant that produces the opium from which heroin can be synthesized, the concentration of opium or heroin in the seeds is not nearly enough to have any kind of neurological (narcotic) effect, compared with taking recreational doses of the drugs. It was possible in the past to fail a drug test if you ate a bagel or two containing poppy seeds. But the minimum concentration needed to fail a drug test has been increased by the FDA to the point where you would not fail the drug test for opium or heroin because you ate some food containing poppy seeds. Anticipating Student Questions (answers to questions students might ask in class) What is the difference between heroin and cocaine? Cocaine is derived from the leaves of the coca plant whereas heroin is extracted from the latex oozing from the poppy plant. Both, however, are alkaloids. Cocaine is essentially a recreational drug, not used to relieve pain as in the case of morphine, though obviously morphine is also used as a recreational drug. Is codeine a narcotic? Is it chemically related to morphine? Codeine is a narcotic, meaning that it is a chemical that dulls the senses. It is made from morphine by a chemical process called methylation by which a methyl group (-CH3) is added to the morphine molecule. Codeine is used to treat lesser pain than that which is treated with morphine (think post-surgical pain). It is also used to suppress coughing (Robitussin A-C), and as a hypnotic (used to induce sleep). Is it true that sodas once included cocaine in the liquid mix? In the early part of the 20th Century, many soft drinks contained cocaine, which is the basis for the name Coca Cola. It is the only drink allowed to keep its name after the U.S. cocaine regulation of 1914 made cocaine illegal. The person who first started the Coca Cola drink, was Dr. John Stith Pemberton of Atlanta, Georgia, who was a morphine addict following injuries in the Civil War. He first started out with a popular concoction in Europe that was based on what is known as French Wine Coca, a mix of both alcohol and cocaine, that produces in the body a third drug called cocaethylene, acting like cocaine but with more euphoria! When the Georgian county in which Dr. Pemberton produced and marketed his drink passed an alcohol prohibition edict, Pembertons French Wine Coca was now illegal because of the alcohol content, not the cocaine! Pemberton replaced the wine with sugar syrup which debuted in 1886 as Coca-Cola, the temperance drink. The coca remained in the drink. It became known as the intellectual beverage among the upper class at that time and was originally served at soda fountains. In 1899, it was sold in bottles and became more widely distributed. For racial reasons, not to be discussed here, the cocaine was removed in 1903, replaced by additional sugar and caffeine. The Coca-Cola of today still contains coca but the ergonine alkaloid is removed. The extraction process is done at a New Jersey chemical processing facility. The coca leaf extract (from 175,000 kilograms of coca per year) is called Merchandise No.5. (source:  HYPERLINK "http://www.theatlantic.com/health/archive/2013/01/why-we-took-cocaine-out-of-soda/272694/" http://www.theatlantic.com/health/archive/2013/01/why-we-took-cocaine-out-of-soda/272694/ )  INCLUDEPICTURE "http://cdn.theatlantic.com/static/mt/assets/food/coca-cola-ad-1886.png" \* MERGEFORMAT  (from  HYPERLINK "http://www.theatlantic.com/health/archive/2013/01/why-we-took-cocaine-out-of-soda/272694/" http://www.theatlantic.com/health/archive/2013/01/why-we-took-cocaine-out-of-soda/272694/) Is marijuana considered to be a narcotic? Marijuana is not an opiate, meaning derived from morphine and therefore is not considered to be a narcotic, pharmacologically speaking. But it is listed as a narcotic by the United Nations so-called Single Convention (1961) which has a world-wide following in terms of international drug control. Originally, the term meant any drug that induces sleep or torpor. In the United States, the term is imprecisely defined and refers to any drug that is totally prohibited or one that is used in violation of strict governmental regulation. In many states, marijuana is classified as a narcotic. In-class Activities (lesson ideas, including labs & demonstrations) The acetylation reaction for converting morphine to heroin can be illustrated by having students synthesize aspirin. A lab procedure with post lab questions and calculations is found at  HYPERLINK "http://chemistry.about.com/od/demonstrationsexperiments/ss/aspirin.htm" http://chemistry.about.com/od/demonstrationsexperiments/ss/aspirin.htm. Students can extract caffeine from coffee. Caffeine is considered to be an addicting drug. The lab procedure can be accessed at  HYPERLINK "http://www.seriaz.org/downloads/4-caffiene.pdf" http://www.seriaz.org/downloads/4-caffiene.pdf. A video that illustrates this particular reaction is found at  HYPERLINK "http://www.youtube.com/watch?v=TvBtRDScKhM" http://www.youtube.com/watch?v=TvBtRDScKhM. Additional reference material including a molecular model of caffeine, its history, and how it is used other than as an ingredient in drinks (soda) is found at  HYPERLINK "http://chemistry.about.com/od/moleculescompounds/a/caffeine.htm" http://chemistry.about.com/od/moleculescompounds/a/caffeine.htm. There are also issues related to the medical use of marijuana for treating various kinds of pain and nausea (from chemotherapy). Some states have legalized the public sale of marijuana with and without medical prescriptions. Is there good scientific evidence to support the medical use of marijuana? And for what purposes? What is the long term effect from using marijuana on a regular basis (recreational)? Are young people more susceptible to any kind of brain damage or impairment from regular use of marijuana? A Teachers Guide (originally created to complement the Educational TV Frontline Program, Drug Wars) provides suggestions for student activities plus background information on treatment and education (versus prohibition and punishment), social justice, the international war on drugs, and the multibillion dollar illegal drug business. Student research would form the basis for student presentations (Power Point) or a debate about a central issueShould marijuana be legalized for medical purposes? or Should marijuana be de-criminalized? Refer to the Web site,  HYPERLINK "http://www.pbs.org/wgbh/pages/frontline/teach/american/drugs/" http://www.pbs.org/wgbh/pages/frontline/teach/american/drugs/. Out-of-class Activities and Projects (student research, class projects) Students can research the issues surrounding the very high rate of imprisonment for people convicted of drug possession and use. There are both moral and financial implications to society for the (some believe) excessive rate of incarceration for certain drug offenses, in particular marijuana usage. This issue would provide the basis for an in-class student debate in which students would have to provide both legal and sociological evidence for their positions. How is alcohol consumption any different from recreational drug usage? Should various drugs be legalized to reduce crime that ensues from drug users having to commit robberies (and often violent attacks) to financially support their drug habit? What kinds of programs exist, free of charge, to help drug addicts break their habits? Shouldnt money be spent on education and treatment rather than on imprisonment? References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  Gottfried, S; Sedotti, M. Horses and Heroin (Mystery Matters). ChemMatters 1988, 6 (3), pp.1415. Presented as a forensic mystery, this article presents a drug bust case (based on a true event) and the chemistry used in the lab, known as the Marquis test, to detect heroin in suspected drug material. Tracey, M. Positive Emission Tomography (PET) Scan. ChemMatters 1994, 12 (1), pp 1315. This detailed and illustrated article relates the use of PET Scans to locate where in the brain various drugs activate the nervous system. In particular, a PET scan allows researchers to elucidate the connection between dopamine neurotransmitters and cocaine, utilizing compounds with radioactive C-11, a very short lived isotope that has to be made just before it is injected into the study patient. Goldfarb, B. Seeds of Doubt. ChemMatters 1995, 13 (2), pp 56. This article complements one of the anticipated student misconceptions about poppy seed ingestion (from a bagel, for example) and its effect on a urine test for morphine. It includes information about fat-soluble and water-soluble drugs and their ability to cross the blood brain barrier. The solubility factor is the basis for the difference in the speed of action of heroin versus morphine. Karabin, S. New Types of Pain Killer From Sea Snails. ChemMatters 2011, 29 (4), p 4. A short brief on the polypeptide structure (with the amino acid sequence) of a toxin taken from sea snails is found in this issue of ChemMatters. (See other Web-based references on the sea snail venom as well as snake venom in the current Teachers Guide.) Web sites for Additional Information (Web-based information sources) More sites on the neurological basis of pain and its management Two complementary articles (previously mentioned in the More on sections of the Teachers Guide, above) that outline a comprehensive approach to treating pain are found at  HYPERLINK "http://harvardmagazine.com/2005/11/relieving-pain.html" http://harvardmagazine.com/2005/11/relieving-pain.html and  HYPERLINK "http://harvardmagazine.com/2005/11/the-science-of-hurt.html" \l "gates" http://harvardmagazine.com/2005/11/the-science-of-hurt.html#gates. Some newer alternatives to morphine that are being investigated for pain management include the use of snake venom. Refer to  HYPERLINK "http://www.scientificamerican.com/podcast/episode.cfm?id=snake-venom-contains-potent-painkil-12-10-04" http://www.scientificamerican.com/podcast/episode.cfm?id=snake-venom-contains-potent-painkil-12-10-04 and  HYPERLINK "http://www.nature.com/nature/journal/v490/n7421/full/nature11494.html" http://www.nature.com/nature/journal/v490/n7421/full/nature11494.html. Another potential source of a pain killer is the venom that can be extracted from a particular species of cone snail. Besides being used to reduce pain, it has potential to treat epilepsy and depression among other neurological disorders. A detailed article can be found at  HYPERLINK "http://www.scientificamerican.com/article.cfm?id=healing-the-brain-with-snail-venom" http://www.scientificamerican.com/article.cfm?id=healing-the-brain-with-snail-venom. Another article explains the biochemistry behind the toxins (which are polypeptides) that are extracted from sea snails. These toxins, now synthesized because the amino acid sequence is known, block important calcium channels in neurons, inhibiting the transmission of pain signals. Pictures of various sea snails that students might recognize from their days at the seashore can be found at  HYPERLINK "https://www.google.com/search?q=sea+snail+vs.+cone+snail&tbm=isch&tbo=u&source=univ&sa=X&ei=kQV0UqvHMqbTsAS9uYCACA&ved=0CHYQsAQ&biw=1414&bih=914" https://www.google.com/search?q=sea+snail+vs.+cone+snail&tbm=isch&tbo=u&source=univ&sa=X&ei=kQV0UqvHMqbTsAS9uYCACA&ved=0CHYQsAQ&biw=1414&bih=914. There is also a video about cone shell venom at  HYPERLINK "http://www.pbs.org/wnet/nature/episodes/the-venom-cure/cone-shell-cures/2061/" http://www.pbs.org/wnet/nature/episodes/the-venom-cure/cone-shell-cures/2061/. An alternate opiate called remifentanil can be used at higher doses than morphine without some of its limitations. Refer to  HYPERLINK "http://www.scientificamerican.com/article.cfm?id=high-dose-opiates-could-crack" http://www.scientificamerican.com/article.cfm?id=high-dose-opiates-could-crack. A very useful reference (which you can adjust for level of explanationbeginner, intermediate, advancedabout the neurological basis of pain and drug action can be found at three sites (from the same basic electronic source); they are  HYPERLINK "http://thebrain.mcgill.ca/flash/d/d_03/d_03_cr/d_03_cr_dou/d_03_cr_dou.html" http://thebrain.mcgill.ca/flash/d/d_03/d_03_cr/d_03_cr_dou/d_03_cr_dou.html,  HYPERLINK "http://thebrain.mcgill.ca/flash/a/a_03/a_03_cr/a_03_cr_dou/a_03_cr_dou.html" http://thebrain.mcgill.ca/flash/a/a_03/a_03_cr/a_03_cr_dou/a_03_cr_dou.html, and  HYPERLINK "http://thebrain.mcgill.ca/flash/i/i_03/i_03_cr/i_03_cr_dou/i_03_cr_dou.html" http://thebrain.mcgill.ca/flash/i/i_03/i_03_cr/i_03_cr_dou/i_03_cr_dou.html. More sites on endorphins and enkephalins A detailed history of the research that went into discovering the existence and role of endorphins and enkephalins is found at  HYPERLINK "http://thebrain.mcgill.ca/flash/d/d_03/d_03_m/d_03_m_dou/d_03_m_dou.html" http://thebrain.mcgill.ca/flash/d/d_03/d_03_m/d_03_m_dou/d_03_m_dou.html. Opals: Playing with Color and Light Background Information (teacher information) More on minerals There are many different definitions of a mineral. The International Mineralogical Association (IMA) has promulgated the following definition in an attempt to provide a succinct statement that meets the needs of mineralogists. In general terms, a mineral is an element or chemical compound that is normally crystalline and that has been formed as a result of geological processes. (Nickel, E. H. The Definition of a Mineral. The Canadian Mineralogist, 1995, 33, pp 689690,  HYPERLINK "http://www.minsocam.org/msa/ima/ima98(04).pdf" http://www.minsocam.org/msa/ima/ima98(04).pdf) Author Nickel, the Vice-Chairman of the IMA Committee on New Minerals and Mineral Names, continues by saying that the above definition suffices to include the vast majority of substances that are generally accepted as minerals. There are some substances, however that do not conform entirely to these requirements. It is, therefore, necessary to consider where the dividing line between mineral and non-mineral should be drawn, and what exceptions to the general statement should be permitted. The paper cited above provides more information on the exceptions. Two other requirements are usually included in the definition of a mineral: crystallinity and stability. Generally, minerals are crystalline, that is, they have an orderly arrangement of their atoms that can be indexed by recognizable and reproducible x-ray diffraction patterns. But some minerals, such as opal, are amorphous, so their atoms are not arranged in an orderly pattern (even though, in opal, there are some areas of crystallinity, there is no overall orderly arrangement of atoms). Some mineralogists are reluctant to grant mineral status to amorphous substances, saying that their composition is not fixed so its impossible to tell if they are truly chemical compounds or mixtures, and their structure is not completely determined. Those scientists prefer to use the term mineraloids for these substances. But some minerals, such as opal, have been grand-fathered in to the minerals list, just because theyve been classified as minerals for such a long time. And in some cases, new technology does allow for more effective study of amorphous phases than had been possible in the past. In particular, Raman and Mossbauer spectroscopy associated with complete chemical analysis are able to unequivocally identify amorphous phases of minerals. The spectroscopic methods can identify 3-dimensional short-range structural environment of the chemical bonds of each element within the structure. In the case of stability, the second requirement for a substances acceptance as a mineral, some substances are metastable, that is, they formed at high pressure or temperature (or both) and their atoms were arranged in the most stable form at those conditions, but that form is not presently the most stable form. They are unable to change to the most stable form at ambient conditions, probably due to a very high activation energy required to make the change. So they are at least temporarily in a stable configurationjust not the most stable configuration. These substances are also accepted as true minerals, if they can be analyzed completely and meet the other criteria necessary for classification as a mineral. There are several other groups of substances that mineralogists study to rule on as minerals (or not), if they meet the stringent criteria. These substances are: extraterrestrial substances (e.g., materials from meteorites or moon rocks, which are generally accepted as minerals because its been determined that they formed from processes similar to those on Earth which produced natural mineralsgeologic processes) anthropogenic substances (e.g., substances produced by man and generally not considered to be minerals); if the man-made substance is identical to a natural mineral, it is referred to as a synthetic equivalent geologically modified anthropogenic substances (e.g., when a geological process such as sea water erosion working on an ancient man-made substance such as metallurgical slags has produced a new substance, this new substance is considered a mineral); going forward, however, new substances produced by geologic action on the plethora of new man-made materials will not be considered to be minerals, according to the International Mineralogical Association biogenic substances, those created by living organisms (e.g., marine animals shells (think diatoms) are not considered minerals, as an unmentioned criteria is that minerals must be abiogenic, that is, not created by living organisms) Opals still qualify because the bacteria found in some opals did not create the opal, they only helped to provide the silicates for opal formation by separating them from the surrounding rocks by providing acid to chemically break down the surrounding rock. According to the IMA there are 4859 known minerals, as of October 2013. For the complete list, see The New IMA List of Minerals A Work in Progress Updated: October 2013. ( HYPERLINK "http://pubsites.uws.edu.au/ima-cnmnc/IMA_Master_List_(2013-10).pdf)" http://pubsites.uws.edu.au/ima-cnmnc/IMA_Master_List_(2013-10).pdf) And there are 16 different status types for minerals, ranging from approved to questionable to hypothetical to not approved. ( HYPERLINK "http://www.mindat.org/glossary/IMA_status" http://www.mindat.org/glossary/IMA_status) More on gemstones A gemstone is defined generally as a rock or mineral that, when cut and polished, can be used for jewelry. This restricted definition would exclude opals, since they are not a type of rock, and they do not have a definite composition and therefore do not fit the definition of a mineral. A slightly broader definition of a gemstone also includes a few non-crystalline (amorphous) materials, such as opal, and a few organic materials, such as pearl, coral and amber. More on opal(s) As has been mentioned above, opal is considered an amorphous substance, yet it does have crystalline structures within the macroscopic material. The  HYPERLINK "http://en.wikipedia.org/wiki/Mineral" \o "Mineral" mineral cristobalite is a high-temperature  HYPERLINK "http://en.wikipedia.org/wiki/Polymorphism_(materials_science)" \o "Polymorphism (materials science)" polymorph of  HYPERLINK "http://en.wikipedia.org/wiki/Silica" \o "Silica" silica, meaning that it has the same chemical formula,  HYPERLINK "http://en.wikipedia.org/wiki/Silicon_dioxide" \o "Silicon dioxide" SiO2, but a distinct crystal structure. Both  HYPERLINK "http://en.wikipedia.org/wiki/Quartz" \o "Quartz" quartz and cristobalite are polymorphs with all the members of the quartz group, which also include  HYPERLINK "http://en.wikipedia.org/wiki/Coesite" \o "Coesite" coesite,  HYPERLINK "http://en.wikipedia.org/wiki/Tridymite" \o "Tridymite" tridymite and  HYPERLINK "http://en.wikipedia.org/wiki/Stishovite" \o "Stishovite" stishovite. The micrometre-scale spheres that make up precious  HYPERLINK "http://en.wikipedia.org/wiki/Opal" \o "Opal" opal are made of cristobalite, crystallized  HYPERLINK "http://en.wikipedia.org/wiki/Metastability_in_molecules" \o "Metastability in molecules" metastably at low temperature. (from  HYPERLINK "http://en.wikipedia.org/wiki/Cristobalite" http://en.wikipedia.org/wiki/Cristobalite) Precious opal shows a variable interplay of internal colors and even though it is a mineraloid, it has an internal structure. At micro scales precious opal is composed of silica spheres some 150 to 300 nm in diameter in a hexagonal or cubic close-packed lattice. These ordered silica spheres produce the internal colors by causing the interference and diffraction of light passing through the microstructure of the opal. HYPERLINK "http://en.wikipedia.org/wiki/Opal" \l "cite_note-Klein-9" [9] It is the regularity of the sizes and the packing of these spheres that determines the quality of precious opal. Where the distance between the regularly packed planes of spheres is approximately half the wavelength of a component of visible light, the light of that wavelength may be subject to diffraction from the grating created by the stacked planes. The spacing between the planes and the orientation of planes with respect to the incident light determines the colors observed. The lattice of spheres of opal that cause the interference with light are several hundred times larger than the fundamental structure of crystalline silica. As a mineraloid, there is no unit cell that describes the structure of opal. Nevertheless, opals can be roughly divided into those that show no signs of crystalline order (amorphous opal) and those that show signs of the beginning of crystalline order, commonly termed cryptocrystalline or microcrystalline opal. Dehydration experiments and infrared spectroscopy have shown that most of the H2O in the formula of SiO2nH2O of opals is present in the familiar form of clusters of molecular water. Isolated water molecules, and silanols, structures such as Si-O-H, generally form a lesser proportion of the total and can reside near the surface or in defects inside the opal. The structure of low-pressure polymorphs of anhydrous silica consist of frameworks of fully corner bonded tetrahedra of SiO4. The higher temperature polymorphs of silica cristobalite and tridymite are frequently the first to crystallize from amorphous anhydrous silica, and the local structures of microcrystalline opals also appear to be closer to that of cristobalite and tridymite than to quartz. The structures of tridymite and cristobalite are closely related and can be described as hexagonal and cubic close-packed layers. It is therefore possible to have intermediate structures in which the layers are not regularly stacked. (from  HYPERLINK "http://en.wikipedia.org/wiki/Opal" http://en.wikipedia.org/wiki/Opal)  INCLUDEPICTURE "http://minerals.gps.caltech.edu/COLOR_Causes/Physical_Process/opal-beads_40k.jpg" \* MERGEFORMATINET  Photomicrograph of ordered layers of spherical silica beads in opal, from the California Institute of Technology (from  HYPERLINK "http://minerals.gps.caltech.edu/COLOR_Causes/Physical_Process/opal-beads_40k.jpg" http://minerals.gps.caltech.edu/COLOR_Causes/Physical_Process/opal-beads_40k.jpg) This list provides the names and descriptions of various types of precious opals that gemologists (and buyers) would be interested in. Opal Types Solid Opals that are cut in a solid piece are known as solids. These are the most valuable. A doublet opals are composed of two layers consisting of an opal top fabricated over opal matrix material, potch (common non-precious opal) basanite, jade, black onyx or obsidian. The value of these are lower than an solid not-assembled composite. A triplet opal has three parts. A piece of precious opal in the centre, a clear top and a darkened base (usually potch or glass) to highlight the colour. Fire Opal is orange or reddish opal from Mexico that has no play of color or iridescence. Boulder Opal results from splitting of a seam of opal running the rock or boulder. The polished piece will have opal as its face and the host rock material as its base. White Opal are white base, more common variety of opals that are usually the least expensive. Black Opal is more rarer [sic] than white and warrant's a higher price. There are various shades of "black" opal. A "Black 3" is a true black color. True  HYPERLINK "http://www.opal33.com/black-opal.html" black opal is simply to die for. Semi-Black Opal are opals with base colors from medium gray to dark gray. Gray Opals have a base color of medium to lighter gray color. Crystal Opal is transparent enough to see through them. Various colors and fire can be seen as light rolls through them. Cut Opal Calibrated and free form cut white, crystal, black opal suitable for ring, brooch, and other settings. Calibrated opals are stated specific sizes in mm. For example a 8 x 10 mm calibrated stone will fit in a purchased settings designed for a 8 x 10 mm stone. Rough Opal Uncut material suitable for free-form or calibrated cutting, ideal for lapidary clubs, hobbyists, custom design work, or inlay work. (from  HYPERLINK "http://www.opal33.com/about-opal.html" http://www.opal33.com/about-opal.html) More on silica As you can see from the following table, taken from Railsbacks Some Fundamentals of Mineralogy and Geochemistry, oxygen and silicon are the two most abundant elements in the Earths crust. Note that this table lists the elements in order of molar per cent, which is somewhat unusual. Abundance and form of the most abundant elements in Earths continental crust  SHAPE \* MERGEFORMAT  Percentages are calculated from data for average continental crust in Appendix III of Krauskopf (1979). For a more recent but less complete compilation, see Taylor and McLennan (1985) The abundances of the first fifteen elements listed add up to 99.77 molar % of average crust. Gold is included solely to allow comparison of these 15 most abundant elements to a very scarce element. Volume percent for oxygen in boldface illustrates the paraphrase by Mason (1958) of the words of Viktor Goldschmidt that the lithosphere may well be called the oxysphere. (table from  HYPERLINK "http://www.gly.uga.edu/railsback/Fundamentals/ElementalAbundanceTableP.pdf" http://www.gly.uga.edu/railsback/Fundamentals/ElementalAbundanceTableP.pdf) And this chart provides a visual interpretation of the eight most abundant elements (by weight %).  INCLUDEPICTURE "http://geology.csupomona.edu/drjessey/class/Gsc101/abundance.gif" \* MERGEFORMATINET  (from  HYPERLINK "http://geology.csupomona.edu/drjessey/class/Gsc101/Mineral.html" http://geology.csupomona.edu/drjessey/class/Gsc101/Mineral.html) Oxygen and silicon constitute 75% of the Earths crust by weight, so it should not be surprising that silica is among the most abundant compounds found on Earth. Seven of eight of the most abundant rock forming minerals are silicates. The Common Rock Forming Minerals Feldspar (silicate) Quartz (silicate) Muscovite (silicate) Ferromagnesians [those minerals containing iron or magnesium] Olivine (silicate) Pyroxene (silicate) Amphibole (silicate) Biotite (silicate) Muscovite (silicate) Calcite (not a silicate) ( HYPERLINK "http://geology.csupomona.edu/drjessey/class/Gsc101/Mineral.html" http://geology.csupomona.edu/drjessey/class/Gsc101/Mineral.html) The most common mineral found on Earth, arguably, is quartz. At least it is the most abundant mineral found in the continental crust. Consider sand, which is quartz that has been weathered into tiny grains. Sand covers all the deserts of the world; it comprises sandstone, and it covers beaches and riverbeds of the worldabundant indeed. But if you include the ocean floor and oceanic crust as part of the Earth, then feldspar wins. Feldspar is a group of seven minerals, usually considered as one. Feldspars formula is XZ4O8, where X is a mixture of potassium, calcium and sodium, and Z is a mixture of silicon and aluminum. Since the oceanic crust contains almost no quartz and abundant feldspars, feldspar is the most abundant mineral in the Earths crust. Note that both quartz and feldspar are silicate minerals, meaning they both contain silicon and oxygen as their main structure. But if you also consider the Earths mantle, the second layer of the Earth, below the crust, then olivine is the most abundant mineral. The mantle is about 1800 miles deep, compared to the crusts depth of only about 22 miles, and the mantle contains primarily olivine. The crust of the Earth compared to the entire Earth is often referred to as being analogous to the skin on an apple. In this analogy, the mantle is similar to the meat of the apple, down to the core (like the Earth) with its seeds (OK, not like the Earth). Therefore, olivine in the mantle is way more abundant than quartz in the continental crust or feldspar in the oceanic crust, making olivine Earths most abundant mineral. But if you include the Earths core, made primarily of nickel and iron, the most abundant mineral is NiFe, a nickel-iron alloy (except it probably cant be considered a mineral, since it doesnt have a definite composition or structure. The outer core is a molten mixture of nickel, iron and The table below from Wikipedia, adapted from one published by the U.S. Geological Survey (USGS), shows actual abundances of many elements found in the Earths crust, according to the number of atoms of each element per 106 atoms of silicon. Note the vertical axis is on a logarithmic scale. Also note that the table is arranged according to atomic number along the horizontal axis. This might be a good table to add to your repertoire when discussing graphical representations of periodic trends in the elements, e.g. densities, ionization energies, etc.  HYPERLINK "http://upload.wikimedia.org/wikipedia/commons/0/09/Elemental_abundances.svg"  INCLUDEPICTURE "http://upload.wikimedia.org/wikipedia/commons/thumb/0/09/Elemental_abundances.svg/774px-Elemental_abundances.svg.png" \* MERGEFORMATINET  Abundance (atom fraction) of the chemical elements in Earth's upper continental crust as a function of atomic number. The rarest elements in the crust (shown in yellow) are not the heaviest, but are rather the siderophile (iron-loving) elements in the Goldschmidt classification of elements. These have been depleted by being relocated deeper into the Earth's core. Their abundance in meteoroid materials is relatively higher. Additionally, tellurium and selenium have been depleted from the crust due to formation of volatile hydrides. (from  HYPERLINK "http://en.wikipedia.org/wiki/File:Elemental_abundances.svg" http://en.wikipedia.org/wiki/File:Elemental_abundances.svg) According to the article accompanying the table above, the transition elements are not as abundant near the Earths surface because they are denser and have been dissolved into the molten nickel-iron molten outer core. In geochemical terms this group of elements is referred to as the siderophile group. Several of the rarest of these elements have been classified as precious metals, e.g. gold and platinum, since the earliest times of man. Even though these elements are relatively rare (and some, extremely so) near the Earths surface, their global (including core) abundances approach those of typical solar abundances throughout space. This implies that the precious metals are relatively abundant in the core. For more information about why elemental abundances are as they are in the table, including the more common elements, see the article Goldschmidt Classification in Wikipedia:  HYPERLINK "http://en.wikipedia.org/wiki/Siderophile_element#Siderophile_elements" http://en.wikipedia.org/wiki/Siderophile_element#Siderophile_elements. More on the uses of silica The following everyday materials use silica in their construction. Sources are all from past issues of Chem Matters. Note that this is just a sampling of materials that use or contain silica. Glass Glass is made by combining sand (Si02), sodium oxide (Na20), and calcium oxide (CaO). The mixture is heated until it melts, then cooled gradually. The silicon and oxygen atoms link together in pyramid-shaped groups containing one silicon and four oxygen atoms. These minute pyramids have a tendency to attach to each other in all directions to form a crystal, but they are blocked from doing so by the sodium and calcium ions. When the mixture cools, the atoms are frozen in a jumbled arrangement. Long chains of pyramids wander through the solid, with Na and Ca ions scattered between the chains. The pyramids give the solid an ordered arrangement over a very short distance (the five atoms of a pyramid). Over longer distances, the solid is disordered. A piece of glass can be considered a continuous solid whose minute orderly units are interconnected randomly. (Robson, D. Breakfast of Crystals. ChemMatters 1983, 1 (3), pp 811) Pyrex Glass Regular glassalso called soda lime glassis made up of about 70% silica (SiO2), about 1015% sodium oxide (Na2O), about 10% calcium oxide (CaO), and small amounts of other minor ingredients. Its LCE [Linear Coefficient of Expansion] is 8.3 parts per million (ppm)/ C. Pyrexwhich is actually a trade name for what is generically called borosilicate glasscontains many of the same components as soda lime glass, but it also contains at least 5% boric oxide (B2O3). The inclusion of boron atoms greatly reduces the LCE of the glass to 3.3 ppm/ C. Because it doesnt expand as much, it is far less likely to break [when heated]. (Becker, R. Question from the Classroom, Part II. ChemMatters 2008, 26 (2), p 2) Computer chips Computer chips must be produced in the cleanest atmosphere possible. The circuit elements on the chip are so tiny that one speck of dust will make a chip useless. The production is done in a clean room. Air is filtered and forced to flow from the ceiling to tiny holes in the floor to force out any particles of dust. Workers wear bunny suits to keep dust from their clothing or skin particles from contaminating the chips. Silicon is purified until it is 99.9999999 % pure: Only one atom in a billion is not silicon. Then a single large crystal of silicon is formed. The silicon is heated to just above its melting point, about 1500 C, and then a tiny seed crystal of pure silicon is dipped into the molten silicon. The seed crystal is rotated and pulled from the mass, and it grows as a single large crystal of silicon. The crystallized silicon is in the shape of a long cylinder, 4 in. (10 cm) in diameter and perhaps 12 in. (30 cm) long. Slices are cut from the cylinder, like slicing bologna, to form the wafers that the chips will be built on. When the silicon wafers are cooked in an oven at 1000 C, they react with the oxygen in the air to form silicon dioxide, SiO2, which is similar to rust on a metal object. The SiO2 does not conduct electricity; this process prevents the chips from short-circuiting once they are finished: Si(solid) + O2(gas) ( SiO2(solid) (Baxter R. Computer Chips: Loaded Bits. ChemMatters 1997, 15 (4), pp 79) Transistors A transistor is similar to a valve in a plumbing system. Just as a valve is used to regulate the flow of water through the plumbing system, a transistor controls the flow of electrical current in a circuit. In the case of a valve, a small tube called the valve stem lets water through; in a transistor, a component called the control gate allows the electrical current to flow (Fig. 3). Transistors use a thin insulating film of silicon dioxide (SiO2) to isolate the control gate from the underlying materialthe part of the transistor where electricity flows, which is made most often of silicon. (Baxter, R. Metals Hidden Strengths. ChemMatters 2009, 27 (3), pp 1112) Toothpaste Todays toothpastes contain milder polishing agents such as silica (SiO2), aluminum oxide (Al2O3), calcium phosphate, or calcium carbonate (CaCO3). (Ruth, C. Teeth Whitening. ChemMatters 2003, 21 (4), pp 79) Deodorant/anti-perspirant The active ingredient of many antiperspirant/ deodorants is aluminum zirconium chlorohydrex gly (anhydrous). Silica (SiO2) is used to provide texture. (Graham, T. Scanning Electron Microscopy Solves a Mystery! ChemMatters 2003, 21 (4), pp 1719) Air bags The nitrogen for the bags inflation comes from a series of chemical reactions. The gas generator contains an electrical ignitor and a precise mixture of sodium azide, NaN3; potassium nitrate, KNO3; and silicon dioxide, SiO2. When ignited, the sodium azide decomposes rapidly and produces sodium metal and nitrogen gas: 2 NaN3 (s) ( 2 Na (s) + 3 N2 (g) The sodium that is set free combines with the potassium nitrate and releases even more nitrogen gas: 10 Na + 2 KNO3 ( K2O + 5 Na2O + N2 Finally, the heat released by these reactions melts the reaction products and SiO2 to form an unreactive, safe glass: K2O + Na2O + SiO2 ( glass (Marsella, G. Airbags: Chemical Reaction Saves Lives. ChemMatters 1997, 15 (1), pp 45) Terra cotta Terra cotta is a heavy material, used for bricks and sewer pipes, as well as for statues and flower pots. It is a ceramic material, which simply means that it is made from clay. More refined ceramics include porcelain, china, and even the thin films used in computers. Clay consists of microscopic particles that are formed from the geological weathering of the rocks and stones at the surface of the earth. In fact, the list of ingredients found in many types of clays resembles the percent composition of the rocks that make up the earths surface. Just like the earths surface, the predominant clay components are silicon dioxide (SiO2) or silica and aluminum oxide (Al2O3) or alumina. To make terra cotta, a sculptor or bricklayer mixes wet clays, shapes them or presses them into molds, and then fires them. Firing at temperatures as high as 1000 C takes place in a special oven called a kiln. This high heat drives off the water of hydration from the surface of the clay particles in a process called sintering and causes it to vitrify or become glasslike. During sintering, a series of complex exothermic reactions occurs. If the clay used is kaolinite, for instance, the overall reaction during firing is 3 (Al2O3" 2SiO2" 2H2O) ' 3 Al2O3" 2SiO2 + 6 H2O Without firing, objects made of clay will disintegrate in water. As the hydrated water on the surface of the clay particles is driven off, new intermolecular attractions between the aluminates and silicates of adjacent particles provide the glue that gives the pottery its strength. (Stone, C. Terra Cotta WarriorsArmy from the Earth. ChemMatters 2000, 18 (1), pp 1415) Silica gel Silica gel, despite its name, is a solid that is chemically unreactive, non-toxic and non-flammable. It is the non-crystalline form of silicon dioxide, SiO2. The crystalline form, sand or quartz, occurs in abundance naturally. Silica gel has a high porosity (about 800 m2/g), which allows it to adsorb (not absorb) about 40 times its own weight in water. This property makes it useful as a cat litter. It is made synthetically from sodium silicate, a compound that exists in several forms. Among these forms are sodium orthosilicate, Na4SiO4; sodium metasilicate, Na2SiO3; sodium polysilicate, (Na2SiO3)n; sodium pyrosilicate, Na6Si2O7. All are water soluble and form alkaline solutions. Silica gel is the desiccant of choice, along with montmorillonite clay and Zeolite. It was developed just prior to World War I for use in gas masks. In World War II it was used to keep penicillin dry. Its also in those little bags that come packed with electronic equipment, for example, to keep moisture out. (from ChemMatters Teachers Guide, October 2004 to supplement article Kitty Litter Chem, 23 (3), pp 1214) Magic Sand If you were to hold beach sand in one hand and magic sand in the other and lower both hands into the water, the beach sand would clearly show individual grains. The magic sand, however, would appear to be surrounded by a silvery layer looking like plastic film. When you lifted your hands out of the water, the beach sand would be wet, with its grains clumping together. The grains of magic sand would not be clumped togetherin fact, they would be perfectly dry! This is because the magic sand was surrounded by a large air bubble; the silvery layer was the curved surface of the bubble. Most sand is impure silica, which has a network of oxygen and silicon atoms. At the surface, the oxygen forms polar covalent bonds with hydrogen atoms. These OH groups carry partial electrical charges that attract similar partial charges in water molecules. The attraction of opposite charges makes water adhere to each grain of sand. Magic sand consists of ordinary sand grains coated with tiny particles of pure silica which have received a special chemical treatment. When the particles are exposed to trimethylhydroxysilane, a reaction takes place between two OH groups. This results in the formation of water, and the bonding of the silane compound to the silica particles. Following this treatment, the exterior of the particle contains CH3 groups that are soluble in oil but are insoluble in water. these molecules [of trimethylhydroxysilane] attach to the microspheres [of silica] and give it a new surface of nonpolar covalent bonds. These bonds do not attract water and thus make the particles hydrophobic water hating. When a few grains of magic sand are sprinkled on water, the polar water molecules attract other polar water molecules so strongly that they prevent the grains of magic sand from breaking through the surface until the layer of sand becomes rather thick. When the magic sand finally sinks, the same surface tension effect also keeps it dry. The air between the grains cannot be forced out because the water molecules will not flow between the hydrophobic grains. However, oil will readily flow between the grains, and magic sand can absorb a surprising quantity of oil. (Robson, D. Magic Sand. ChemMatters 1994, 12 (2), pp 89) Diatomaceous earth (This use of silica is not from ChemMatters.) When diatoms (see More on diatoms, below), whose shells are composed primarily of silica, die and decay, they sink (albeit slowly) to the ocean or river or lake bottom and form thick layers of sediment. Under compression over geological time spans, this sediment becomes a rock layer. This rock, essentially the shells of silica from all those diatoms, is referred to as diatomaceous earth (DE). It is natural and relatively safe, so it has many uses: For pest controlthe diatomaceous earth is spread around areas where insects are known to breed. The broken, glassy shells of the tiny diatoms are sharp and cut into the exoskeletons of the insects, causing them to undergo dehydration and death; (it feels soft and powder-like to humans, but NOT to insects) As an additiveto polishes facial scrubs and toothpaste, since it has abrasive qualities As a filtering mediumfor swimming pools, it is able to trap dirt in the small pores between the rock particles; a different grade of DE is used for pool filters, one that has been heat-treated to contain a larger percentage of crystalline silica As an absorbentin cat litter (it is also used to clean up toxic spills by the Centers for Disease Control and Prevention) In agricultureadded to feed to kill insects and used to de-worm animals; its added to their feed and they ingest it (it doesnt hurt themin fact, it is believed that it may have nutritive qualities, perhaps arising from its anti-caking ability that prevents feed from caking, thus providing more feed surface area to digestive juices, possibly resulting in better, more complete digestion) As a dessicantit can absorb more than its own weight in water As a stabilizer for nitroglycerinethis is what Alfred Nobel used to stabilize nitroglycerine in the process of making dynamite Note that any of the above uses of DE for human or animal consumption or direct exposure is primarily amorphous silica, as opposed to crystalline silica. More on the structures of silica and silicates  As mentioned in the article, silica forms tetrahedral building blocks like the one at the right, within its structures. These tetrahedra can link in basically six different ways to create structures with very different properties. For example, in quartz, the SiOSi bond forms an angle of 144o (see below).  The SiO4 tetrahedron (from  HYPERLINK "http://www.quartzpage.de/gen_struct.html" http://www.quartzpage.de/gen_struct.html)  (from  HYPERLINK "http://www.quartzpage.de/gen_struct.html" http://www.quartzpage.de/gen_struct.html) This fundamental structural unit [has] four negative charges. It is found in all silicate minerals (ie. amphibole, olivine, pyroxene, quartz, feldspar, etc.). The silica tetrahedra may be arranged in chains (ex: pyroxenes), double chains (ex. amphiboles), sheets (ex: micas), and frameworks (ex: quartz, feldspars), forming the basic structure of the planet. (from  HYPERLINK "http://www.ldeo.columbia.edu/edu/dees/ees/glossary/periodic_table_lith/silica_tetra.html" http://www.ldeo.columbia.edu/edu/dees/ees/glossary/periodic_table_lith/silica_tetra.html) The table below comes from web.VisionLearning.com. Four of the six arrangements of the silica tetrahedra are shown. The left diagram shows the tetrahedral model, and the right diagram shows the ball and stick model of the same structure. Clicking on the animations above will take you to the VisionLearning Web page to show the structure in 3-D.  INCLUDEPICTURE "http://web.visionlearning.com/silica_tetra_triangle2.jpg" \* MERGEFORMATINET Silica tetrahedron  HYPERLINK "http://web.visionlearning.com/silica_molecule.html" animation INCLUDEPICTURE "http://web.visionlearning.com/silica_tetrahedron_molecule.jpg" \* MERGEFORMATINET  Single chain structure  HYPERLINK "http://web.visionlearning.com/silica_chain.html" animation INCLUDEPICTURE "http://web.visionlearning.com/silica_tetra_singlechain5.jpg" \* MERGEFORMATINET Double chain structure  HYPERLINK "http://web.visionlearning.com/silica_double_chain.html" animation INCLUDEPICTURE "http://web.visionlearning.com/silica_sheet.jpg" \* MERGEFORMATINET Sheet silicate structure  HYPERLINK "http://web.visionlearning.com/silica_sheet.html" animation INCLUDEPICTURE "http://web.visionlearning.com/silica_quartz.jpg" \* MERGEFORMATINET  Framework silicate structure  HYPERLINK "http://web.visionlearning.com/silica_quartz.html" animation  (from  HYPERLINK "http://web.visionlearning.com/silica_molecules.shtml" http://web.visionlearning.com/silica_molecules.shtml) Here are two structures showing crystalline quartz and amorphous glass, both made of silica. Note the regular structure of the quartz, and the relatively random arrangement of the SiO2 units in glass.  INCLUDEPICTURE "http://steelguru.com/uploads/reports/sss1-29-08-2008.jpg" \* MERGEFORMATINET  (from  HYPERLINK "http://www.steelguru.com/article/details/MjU%3D/Solid_State_Structure.html" http://www.steelguru.com/article/details/MjU%3D/Solid_State_Structure.html) The following atomic-level images require the Jmol add-on to view. ( HYPERLINK "http://jmol.sourceforge.net/" http://jmol.sourceforge.net/) (See description of Jmol in General Web References at end of Teachers Guide.) Most can be viewed in ball-and-stick, space-filling and polyhedral viewing modes. They can be rotated, zoomed in and out, and made to spin along different axes. Atomic-level Jmol image of alpha-quartz, silica, SiO2,  HYPERLINK "http://virtual-museum.soils.wisc.edu/alpha-quartz/index.html" http://virtual-museum.soils.wisc.edu/alpha-quartz/index.html Atomic-level Jmol image of Crystobalite, silica, SiO2, polymorph of quartz:  HYPERLINK "http://virtual-museum.soils.wisc.edu/cristobalite/index.html" http://virtual-museum.soils.wisc.edu/cristobalite/index.html Polyhedral view shows pyramids/tetrahedra and how they are linked. Atomic-level Jmol image of kaolinite, Al2Si2O5(OH)4 (a clay mineral) Although not just silica (it also contains aluminum and hydroxyl groups), it has much the same structure. It contains tetrahedral layers of silica, as well as octahedral layers of alumina. The pyramids in the polyhedral viewing mode show different linkages, along edges (alumina) and corners (silica) of the pyramids, instead of just at corners, as in pure silica. The individual layers can be isolated to show students. ( HYPERLINK "http://virtual-museum.soils.wisc.edu/kaolinite/index.html" http://virtual-museum.soils.wisc.edu/kaolinite/index.html) More on diatoms "Such perfect architects, these diatoms. They spin themselves intricate houses of opal in the sea." Richard B. Hoover, from Those Marvelous, Myriad Diatoms, National Geographic, June 1979 You may wonder why I included the topic of diatoms in this Teachers Guide. The fact of the matter is that diatoms use silica dissolved in ocean (and fresh) water to construct a shell-like silica structure around themselves. As explained in Microbe Worlds Meet the Microbes, Whatever their shape, all diatoms have shell-like, brittle cell walls made out of silica (glass) and pectin. The walls are two interlocking halves or shells that fit together like a pillbox. Because they depend on sunlight for photosynthesis, diatoms generally live in the upper 200 meters of oceans and bodies of fresh water. Some species of diatoms simply float in the water currents near the surface. Others attach themselves to larger floating objects or to the sea floor. When diatoms die, they slowly sink to the sea floor. The buildup of trillions of these shells forms a crumbly white sediment known as diatomaceous earth or diatomite, which is used in manufacturing pool filters and abrasives, including toothpaste. ( HYPERLINK "http://archives.microbeworld.org/microbes/protista/algae/diatoms.aspx" http://archives.microbeworld.org/microbes/protista/algae/diatoms.aspx) To give the reader an idea of the intricacies of and variations among diatoms, here are a few photos.  INCLUDEPICTURE "http://www.daviddarling.info/images/diatoms.jpg" \* MERGEFORMATINET  diatoms (from  HYPERLINK "http://www.daviddarling.info/encyclopedia/D/diatom.html" http://www.daviddarling.info/encyclopedia/D/diatom.html)  HYPERLINK "http://upload.wikimedia.org/wikipedia/commons/2/29/Diatom2.jpg"  INCLUDEPICTURE "http://upload.wikimedia.org/wikipedia/commons/thumb/2/29/Diatom2.jpg/600px-Diatom2.jpg" \* MERGEFORMATINET  Circle of diatoms on microscope slide (from  HYPERLINK "http://en.wikipedia.org/wiki/File:Diatom2.jpg" http://en.wikipedia.org/wiki/File:Diatom2.jpg) And here is a bit more information about diatoms: Diatoms are made of soft organic materials encased within a hard opaline shell. The shell, which is called the frustule, is a transparent glassy structure that comes in many fascinating and beautiful shapes. In fact, the intricate architecture of the frustule is what scientists use to distinguish one diatom species from another. The composition of the frustule is very similar to the gemstone opal. They both contain the elements silicon and oxygen along with water molecules. Diatoms grow best where silica, as well as sunlight and other nutrients, are plentiful. There are two parts to the diatom's frustule. Each part is a shallow, half-cylinder called a valve. Since one of the valves, the epitheca, is slightly smaller than the other valve, the hypotheca, the two parts fit together like a pill box, encasing the protoplasm inside. However, due to their extreme ornamentation, it is sometimes impossible to distinguish the epitheca from the hypotheca from the fixed view of a single photograph. ( HYPERLINK "http://earthguide.ucsd.edu/diatom/d3.html" http://earthguide.ucsd.edu/diatom/d3.html) The picture at the right is a case where it is possible to distinguish the epitheca from the hypotheca from a single photograph. Electron photomicrograph showing the two frustules of a centric diatom collected from the clay lining of a Trypaea australiensis (yabby) burrow half a metre below the sediment surface in the Richmond River estuary at Ballina, N.S.W. The lower frustule is filled with very small (H" 1m) euhedral pyrite crystals. T. Australiensis is a burrowing crustacean that is common in intertidal and shallow sub-tidal sandbars and beaches in estuaries on the east coast of Australia; anglers commonly use it for bait. The burrows are oxygenated and burrow wall sediment is oxidised when the yabbies push water through their burrows with their pleopods, but locally reducing conditions can develop within this oxidized sediment. In this example, highly reducing conditions have developed in the interior of a decomposing diatom and pyrite crystals have formed when sulphide produced by sulphate-reducing bacteria has combined with dissolved iron from pore-waters. The two halves of the diatom opened when the electron beam in the microscope first hit the sample near the diatom. The image [above, right] was produced on a Leica scanning electron microscope by Richard Bush using material collected during a study of T. Australiensis burrow chemistry by Geoff Kerr; both are post-graduate students at Southern Cross University, Lismore. Sent by Dr David McConchie ( HYPERLINK "http://www.fromage.ethz.ch/photo.html" http://www.fromage.ethz.ch/photo.html) Note that the above photograph is atypical. The two bivalve halves were separated by the high-energy electron beam when the picture was taken. The photo to the right is much more typical of the way diatoms look in real life. This one still has the two halves, the epitheca and the hypotheca, but they are sealed together and much more difficult to distinguish. (from  HYPERLINK "http://gtresearchnews.gatech.edu/images/diatom_md.jpg" http://gtresearchnews.gatech.edu/images/diatom_md.jpg) When diatoms die, they decompose and decay, leading to organic and inorganic (silicates) sediment. Since this sediment is trapped in clays and silts, it forms a permanent geological record. The inorganic part can be used to analyze marine environments of the past by taking cores of ocean floods or mud from bays. More on synthetic opals (vs. simulants, doublets and triplets) Synthetic opals are gems made by man, not by nature. These opals are still made of silica, but through a man-made process. In the 1970s, the Gilson Company developed a three-step process to make convincing synthetic opal. First, microscopic spheres of silica are created through precipitation. Next, the spheres settle in acidic water for more than a year. Finally, a hydrostatic press consolidates the spheres without distorting the stacked arrangement that creates opals play-of-color.  INCLUDEPICTURE "http://www.gia.edu/cs/Satellite?blobcol=gfile&blobheader=image%2Fjpeg&blobkey=id&blobtable=GIA_MediaFile&blobwhere=1355958489525&ssbinary=true" \* MERGEFORMATINET  These synthetic opals are sometimes seen in the market, and to the unpracticed eye may appear to be highly valuable, natural white and black opal. (from  HYPERLINK "http://www.gia.edu/gem-synthetic" http://www.gia.edu/gem-synthetic) These opals are, at least, still made of opal material, so they are referred to as synthetic opals. But not all opals on the market are natural or synthetic. In some cases, people working in the gem industry will produce and sell fake or imitation opals. In gemologist jargon, these are known as simulants. These pieces are made of glass or plastic or some other substance and have almost no actual value as a gemstone, except to a potential buyer who may or may not know theyre not real opals.  INCLUDEPICTURE "http://www.gia.edu/cs/Satellite?blobcol=gfile&blobheader=image%2Fjpeg&blobkey=id&blobtable=GIA_MediaFile&blobwhere=1355958463489&ssbinary=true" \* MERGEFORMATINET  A glass material known as slocum stone can mimic the appearance of opal. (from  HYPERLINK "http://www.gia.edu/gem-imitation" http://www.gia.edu/gem-imitation) Doublets are gems (the real thing) which have a piece of the gemstone attached to a layer of a background substance (not the real thing) which sets off its beauty and gives it strength. This layer could be a natural matrix or glass or plastic. Triplets are gems which have a piece of the gemstone sandwiched between a backing layer similar to that of a doublet and a cover layer that is usually transparent and lends protection to the gem layer. Both of these types of man-made gems decrease the value of the gemstone substantially, compared to a solid gemstone of the same size. Their value is in making a virtually useless piece of the gemstone (due to its fragility or shape) saleable. Doublets and triplets in opals are fairly common. Although these opals contain other minerals or substances besides opal, they also contain a piece of an opal, usually too thin or fragile to stand alone as a gem. The extra pieces are glued on to the front and/or back of a piece of opal to enhance the stability/longevity of the opal, as seen below.  INCLUDEPICTURE "http://www.gia.edu/cs/Satellite?blobcol=gfile&blobheader=image%2Fjpeg&blobkey=id&blobtable=GIA_MediaFile&blobwhere=1355958457566&ssbinary=true" \* MERGEFORMATINET  A triplet contains two or more segments of a gem, or different gems, that are joined by layers of glue. In a profile view, this image shows a thin seam of opal in the center that is backed by dyed black chalcedony, and is overlayed (the domed area) by a quartz cabochon. (from  HYPERLINK "http://www.gia.edu/gem-imitation" http://www.gia.edu/gem-imitation) Although doublets and triplets are used to imitate natural gems, assembled stones are not always imitations. This is the case with natural opal, which sometimes occurs in layers so thin that they need reinforcement to be sturdy enough for jewelry use. Black onyx, plastic, or natural matrix have served as the bottom layers of opal doublet or triplet cabochons. Opal triplets are topped with a transparent dome made of rock crystal, plastic, glass, or synthetic corundum. (from  HYPERLINK "http://www.gia.edu/gem-imitation" http://www.gia.edu/gem-imitation) More on polymer opal films Amy Winters, the designer of Rainbow Winters fashions, one of whichthe polymer opal filmwas spotlighted in the article, has several science- and technology-based lines of fashions. Rainbow Winters gives the 'wow' factor to the entertainment, fashion and advertising industries with interactive wearable design. Creating a touch-sense-sound multisensory experience. Rainbow Winters has a radically different approach fusing the cutting edge of science with the high-art of fashion to create visually stunning pieces especially made for music videos, rock-concerts, award-ceremonies, advertisements, magazine editorial and red-carpet events. Rainbow Winters seeks to express the emotive and aesthetic capabilities of emerging technologies through illuminated textiles, sensors, colour-changing inks & nanotechnology. Experimentally merging technology with fashion, clothes use interactive textiles which change colour and pattern in response to sound, sunlight, water and stretch. Rainbow Winters represents the cutting edge, experimental, boundary pushing force of interaction design and technology. ( HYPERLINK "http://www.rainbowwinters.com/about.html" http://www.rainbowwinters.com/about.html) This link,  HYPERLINK "http://www.colours.phy.cam.ac.uk/synthetic-opals/" http://www.colours.phy.cam.ac.uk/synthetic-opals/, referenced in the opals article, describes briefly how polymer opals are formed and how they work. At the bottom of the Web page is a brief 14-second video clip showing the change in color from yellow to green to blue and back again upon stretching and relaxing a polymer film. Another <1-minute video clip from the University of Cambridge shows a similar piece of plastic being stretched and relaxed:  HYPERLINK "http://vk.com/video-23304496_165270070" http://vk.com/video-23304496_165270070. More on photonic crystals Photonic crystals function with light the same way semiconductors do with electricity. Semiconductors operate with an electronic band-gap, a narrow range of energies which no electron can occupy. When the electrons in the semiconductor fill all the states available to them below the band gap, electric current cannot flow, because each electron has nowhere to go. Boosting an electron above the gap takes a lot of energy. If there are a few excess electrons, however, they automatically must sit above the gap, where they can easily roam through the wide open spaces of empty states. Similarly, a deficit of electrons opens up some positively charged holes below the gap, again providing a way for current to flow readily. All the magic of semiconductorsthe switching and logic functionscomes about from controlling the availability of electrons and holes above and below the band gap. The existence and properties of an electronic band gap depend crucially on the type of atoms in the material and their crystal structurethe spacing and shape of the lattice that they form. By substituting various other atoms (called dopants) into the lattice or its interstices, engineers can dictate the number of electrons or holes in the semiconductor and thereby tailor its properties. In silicon and other semiconductors, adjacent atoms are separated by about a quarter of a nanometer. Photonic band gap materials involve similar structures but at larger scales. A typical example would be a block of special glass drilled through with a closely spaced array of cylindrical holes, each with a diameter of 400 nanometers. These openings are analogous to the atoms in a semiconductor. In general, but not always, the spacing of the array must be reasonably close to the wavelength of the light or the electromagnetic waves to be controlled. Visible light has wavelengths ranging from about 400 to 700 nanometers; many cell phones use waves around 35 centimeters long. Light entering the holey material will refract through and partially reflect off the myriad internal interfaces between air and glass. The complex pattern of overlapping beams will reinforce or cancel one another out according to the lights wavelength, its direction of travel through the crystal, the refractive index of the glass, and the size and arrangement of all the holes. Perfect cancellation in all directions for a narrow band of wavelengths is like the band gap for electrons in semiconductors: that band of light cannot propagate through the crystal. Modifying the band gap structurefor instance, by filling some holesproduces other effects, similar to what can be done by doping electronic semiconductors. Often a photonic crystal is made of an electronic semiconductor material, and so the crystal has both an electronic band gap and a photonic band gap. (from Yablonovitch, E. Photonic Crystals: Semiconductors of Light. Scientific American December 2001,  HYPERLINK "http://optoelectronics.eecs.berkeley.edu/eliy_SCIAM.pdf" http://optoelectronics.eecs.berkeley.edu/eliy_SCIAM.pdf) Polymer opal films are one type of photonic crystal. According to the Optical Society of America, These polymer opal films" belong to a class of materials known as photonic crystals. Such crystals are built of many tiny repeating units, and are usually associated with a large contrast in the components optical properties, leading to a range of frequencies, called a "photonic bandgap," where no light can propagate in any direction. Instead, these new opal films have a small contrast in their optical properties. As with other artificial opal structures, they are also "self-assembling," in that the small constituent particles assemble themselves in a regular structure. But this self-assembly is not perfect, and though meant to be periodic, they have significant irregularities. In these materials, the interplay between the periodic order, the irregularities, and the scattering of small inclusions strongly affect the way the light travels through these films, just as in natural opal gem stones, a distant cousin of these materials. For example, light may be reflected in unexpected directions that depend on the light's wavelength. Photonic crystals have been of interest for years for various practical applications, most notably in fiber optic telecommunications but also as a potential replacement for toxic and expensive dyes used for coloring objects, from clothes to buildings. Yet much of their commercial potential has yet to be realized because the colors in manmade films made from photonic crystals depend strongly on viewing angle. If you hold up a sheet of the opal film, Baumberg explains, Youll only see milky white, unless you look at a light reflected in it, in which case certain colors from the light source will be preferentially reflected. In other words, change the angle, and the color changes. (from  HYPERLINK "http://www.eurekalert.org/pub_releases/2007-07/osoa-pof072307.php" http://www.eurekalert.org/pub_releases/2007-07/osoa-pof072307.php) More on structural color COLR Technologys Web site briefly describes their process of using iron oxide superparamagnetic Colloidal Nanocrystal Clusters (CNCs) to make individual particles self-assemble to form a microscopic, lattice-like structure which diffracts specific wavelengths of light. Adjusting the strength of the magnetic field tunes the color to display brilliant, iridescent colors across the entire visible spectrum and beyond. ( HYPERLINK "http://colrtechnology.com/index.php" http://colrtechnology.com/index.php) More on constructive and destructive interference Lest you think that constructive and destructive interference only occurs with light, we offer the following: The soul of every NASCAR car is its engine. NASCAR cars have 8 cylinders, as do the largest and most powerful passenger vehicles. A cylinder is a space within an engine where the piston moves up and down. You can tell how many cylinders an engine has by the number of spark plugs. Each cylinder contains one spark plug. If the 8 cylinders are arranged in a V pattern, the engine is a V-8 (which has nothing to do with the vegetable juice). The V shape is designed to reduce engine vibration, as the vibrations that the pistons produce are cancelled out through destructive interference. The V shape maximizes this destructive interference, thus reducing engine vibration. (Rohrig, B. Chemistry on the Fast Track: The Science of NASCAR. ChemMatters 2007, 25 (1), pp 56) Actually, active noise control, as found in noise cancelling headphones (think Bose) and similar devices also work on the principle of destructive interference. They generate wavelengths of sound that are 180o out of phase (crest to trough, trough to crest) with wavelengths of incoming noise, thus destructively interfering the incoming noise and cancelling out its sound (see diagram below from Wikipedia). For more information, see How Noise-cancelling Headphones Work on the HowStuffWorks Web site at  HYPERLINK "http://electronics.howstuffworks.com/gadgets/audio-music/noise-canceling-headphone.htm" http://electronics.howstuffworks.com/gadgets/audio-music/noise-canceling-headphone.htm.  HYPERLINK "http://upload.wikimedia.org/wikipedia/commons/7/7d/Active_Noise_Reduction.svg"  INCLUDEPICTURE "http://upload.wikimedia.org/wikipedia/commons/thumb/7/7d/Active_Noise_Reduction.svg/284px-Active_Noise_Reduction.svg.png" \* MERGEFORMATINET  Connections to Chemistry Concepts (for correlation to course curriculum) Molecular structure and compositionIn silicon dioxide, silicon with its four valence electrons forms tetrahedral arrangements of the atoms, resulting in a network solid. Solubility and solutionsAmorphous silica dissolves (more readily than crystalline silicaquartz), and the solution trickles down through porous rock, until it meets an impermeable layer, where it settles. Water evaporates out, leaving a more concentrated solution, until the concentration exceeds the solubility of the silica, whereupon it precipitates to form silica spheres, which then stack uniformly to produce opal. This is a great example of precipitate formation from solution. Periodicity and bondingAlthough students might expect carbon and silicon to have identical (or very similar) chemistry, carbon can form double bonds with oxygen to form single molecules of CO2, while silicon does not display double bonding with oxygen, instead forming single bonds with four oxygen atoms resulting in the network solid silica. This is due to atomic size and electronegativity of the atoms involved. Light and colorOpals exhibit play of color, due to the arrangement and spacing of the spherical layers of silica within the structure. This is easily related to wavelengths of the various colors of light when studying the electromagnetic spectrum. BondingThe way various tetrahedral bond to one another in silica-based minerals determines their properties. Crystal structureQuartz is a crystalline substance, while opal is amorphous. Comparisons can be made here re: hardness, melting point, etc. between amorphous and crystalline substances; e.g., quartz is very hard (7 on Moh hardness scale), while opal is softer (5.56.5). Possible Student Misconceptions (to aid teacher in addressing misconceptions) Opals must be really rare because theyre gemstones and theyre called precious opals. Actually, this is only partly a misconception. Precious opals are indeed gemstones and actually are rather rare, but common opals are just thatreally common, especially if you note that diatoms have opaline shells (and diatoms are among the most abundant organic life on earth). Also, rarity, although it is a major consideration, is not the only factor that contributes to a minerals (see next misconception) being classified as a gemstone. One must also consider appearancecolor, transparency, luster, purity, etc.as well as hardness (above 6 on the Moh hardness scale), durability, and chemical and physical stability to resist corrosion. And dont forget that size counts! For opals specifically, three other factors contribute to their value: base color, dominant color, and color pattern (see More on opals in the Background Information section, above. Opals are rare minerals. This misconception may sound like the last one but it is based not on the word rare, but on the word mineral. Strictly speaking, opals are not considered to be minerals because they are not crystalline. They are sometimes called mineraloids, although most mineralogists today accept that opals are minerals. Anticipating Student Questions (answers to questions students might ask in class) If silica and sand are both made of silicon dioxide, and if silica can dissolve in water to form silica solutions from which opals can form, why doesnt sand dissolve in ocean water? Silica and quartz are polymorphsthey have the same chemical formula, SiO2, but different crystalline structures. The solubility of silicon dioxide in water greatly depends on its crystalline form and is 3 to 4 times higher for silica than quartz. How can a silicon dioxide molecule have four oxygen atoms around it, when its formula is SiO2? Although there are four oxygen atoms surrounding each silicon atom in the three-dimensional network solid, only two of them are from the original molecule of silicon dioxide. The other two oxygen atoms come from other silicon atoms within the three-dimensional structure. And though there is a very small difference in bond distances between the two oxygen atoms that originate with that silicon atom and the two oxygen atoms coming from different silicon atoms, for all practical purposes, the four bonds are identical. The two bonds between the extra oxygen atoms and the central silicon atom can occur due to the unused valence electrons in silicon and oxygen. If each oxygen atom only uses one bonding (unpaired) electron to bond with its silicon atom, it has one unpaired electron left to use to bond with a neighboring silicon atom. Similarly, each silicon atom uses two bonding (unpaired) electrons to bond with its two oxygen atoms. Then it uses its remaining two valence electrons (now unpaired, assuming promotion) to bond with two more oxygen atoms (a total of four oxygen atoms) to extend the three-dimensional solid network of bonds. Are silica, silicon, and silicone all the same thing? No. Heres a succinct comparison of the three substances: Silica The term silica is used to refer to a group of minerals that contain the elements silicon and oxygen. Opal is considered to be a type of silica. Thus, you might read or hear that diatom shells are composed of either opal or silica, and that their shells are opaline or siliceous. Silicon The mineral matter silica and the element silicon are not synonymous. The element silicon is the silvery material used to make computer chips. It is also the second most abundant element in the earth's crust. Silicone The term silicone refers to an entirely different chemical compound, a polymer that also contains the element silicon. Rubbery silicone is commonly used in caulking for bathtubs and sinks. ( HYPERLINK "http://earthguide.ucsd.edu/diatom/d3.html" http://earthguide.ucsd.edu/diatom/d3.html) In-class Activities (lesson ideas, including labs & demonstrations) You can have students prepare a chemical garden of silicate compounds using sodium silicate (water glass) and various water-soluble transition metal compounds. The garden illustrates the relatively low solubility of silicate compounds. Here is one site with directions:  HYPERLINK "http://www.nuffieldfoundation.org/practical-chemistry/making-crystal-garden" http://www.nuffieldfoundation.org/practical-chemistry/making-crystal-garden. And here is an 8-minute video on chemical gardens from the University of Nottingham video series Periodic Videos. The video shows close-up views of a very nice garden in time-lapse photography, and then shows similar experiments grown on the space shuttle (which didnt turn out as nice). ( HYPERLINK "http://www.youtube.com/watch?v=sAr7mvBjWq8" http://www.youtube.com/watch?v=sAr7mvBjWq8) This site is a PowerPoint presentation, Fundamentals of Crystal Chemistry, that could be used in a classroom presentation. The slides include a discussion of elements found in the Earths crust. Data is included showing oxygen to be the most abundant element in substances found in the crust. The show develops the main types of bonding: ionic, covalent, metallic and secondary, and their roles in forming crystals. It concludes with Paulings 5 rules of crystal formation, focusing on geometry and radii of ions. ( HYPERLINK "http://www.people.carleton.edu/~bhaileab/mineralogy/MinWeb/ChrystalchemistryLecture.ppt" http://www.people.carleton.edu/~bhaileab/mineralogy/MinWeb/ChrystalchemistryLecture.ppt) The October 1990 ChemMatters Classroom Guide (the predecessor to the present-day Teachers Guide) describes a procedure to construct a spectroscope from a piece of diffraction grating, a pizza box and a 35 mm film canister (possibly still available free from camera shops). When studying bonding of hydrogen and the oxygen family members, we usually discuss the oxygen-family hydrides as being unusual in that their boiling points dont follow their molar masses. (H2O should be the lowest boiling point, then H2S, H2Se and H2Te, in that order.) You might want to discuss the following statement from the quote regarding the table of elemental abundances vs. atomic number, in Background Information above: The rarest elements in the crust (shown in yellow) are not the heaviest, but are rather the siderophile (iron-loving) elements in the Goldschmidt classification of elements. These have been depleted by being relocated deeper into the Earth's core. Their abundance in meteoroid materials is relatively higher. Additionally, tellurium and selenium have been depleted from the crust due to formation of volatile hydrides. [Bolding, mine] What if H2O did have the lowest boiling point? ( HYPERLINK "http://en.wikipedia.org/wiki/File:Elemental_abundances.svg" http://en.wikipedia.org/wiki/File:Elemental_abundances.svg) There is quite a bit of chemistry and Earth history in the distribution of elements in/on the Earth. The Goldschmidt article from Wikipedia (#4, above) contains information one could use to discuss in class why elements are found where they are in/on the Earth. This site contains a lengthy, 15-page model-building lab activity for students. They make a series of paper tetrahedra from templates on the document. Then they assemble them according to the various ways that silica tetrahedra arrange themselves in different structures that include most of the rocks and minerals on/in the Earths crust. The activity is actually for a geology course, but it fits right in with the topic of opals. It shows students how to link the tetrahedral to make isolated tetrahedral silicates, ring silicates, single chain silicates, double chain silicates, sheet silicates and network silicates. ( HYPERLINK "http://academic.brooklyn.cuny.edu/geology/powell/courses/geol7040/GEOL7040-Silicate%20Structures%20Activity.pdf" http://academic.brooklyn.cuny.edu/geology/powell/courses/geol7040/GEOL7040-Silicate%20Structures%20Activity.pdf) Out-of-class Activities and Projects (student research, class projects) For students interested in crystal structures (with or without the mineral connection), students could prepare models of the various types of crystal arrangements. This site provides templates for students to use to build paper models of each crystal shape:  HYPERLINK "http://webmineral.com/crystall.shtml" http://webmineral.com/crystall.shtml Students could compare/contrast the crystalline structures of the various silicon-containing minerals. They can use the site in #1 above, and they can find the chemical composition of any of the International Mineralogical Association (IMA) list of 4858 minerals in a pull-down list at the following Web site:  HYPERLINK "http://rruff.info/ima/#" http://rruff.info/ima/#. You can search by content or by name. A clickable periodic table is also available on this same page that allows the student to choose an element and find a list of all the minerals that contain that element. More information about the minerala photo, its powder x-ray diffraction data and Raman spectrographcan be found at  HYPERLINK "http://rruff.info/" http://rruff.info/. This page works the same way as the Web page listed above. Students could research and report on individual gemstone structures and properties and compare their findings to those of opal. References (non-Web-based information sources)  SHAPE \* MERGEFORMAT  Harwood, W. The Color of Gems. ChemMatters 1988, 6 (4), pp 79. The author focuses on the colors exhibited by the mineral alexandrite and discusses what colors other gemstones exhibit, and why they have these colors. It includes a brief discussion of wavelengths of light and the structures of corundum and ruby. Zaugg, H. Growing Diamonds. ChemMatters 1990, 8 (2), pp 1013. The author discusses differences between graphite and diamond, including their relative stability and changing from one to the other by chemical/geological means. He includes a description of creating diamonds by chemical vapor deposition. Baxter, R. Glass: An Amorphous Solid. ChemMatters 1998, 16 (3), pp 910. Author Baxter discusses the structure, composition and manufacture of glass. Wood, C. The Two Faces of Carbon. ChemMatters 2004, 22 (4), pp 46. This article discusses the structural differences between graphite and diamond (another gemstone). Rohrig, B. Glass: More Than Meets the Eye. ChemMatters 2006, 24 (3), pp 48. This article discusses the properties of glass that make it useful as evidence in crime scene analysis. It includes a student activity on page 8 using glycerol to make disappearing glass. The Teachers Guide for the October 2006 issue of ChemMatters (above) contains detailed background information about glass to accompany the article Glass: More Than Meets the Eye The guide includes a description of the many types of glass and the ways glass is used in crime scene analysis. Sicree, A. Graphite versus Diamond: Same Element but Different Properties. ChemMatters 2009, 27 (3), pp 1314. The author discusses the structural differences between the two allotropes of carbon and the sources of diamonds. Baxter, R. Crystalline Structure/Periodic Table: The Beauty of Impurities. ChemMatters 2013, 31 (1), p 4. This is a brief, half-page article describing the role impurities play in the colors displayed by gemstones. It includes mention of electron jumps and the colors of transition metals. ____________________ The February 1999 issue of National Geographic contains an article, Diatoms, Plants with a Touch of Glass, by Carlyne Murawski that shows some nice scanning electron microscope photos of diatoms. It describes diatoms role in the natural food cycle and in the production of oxygen. It also discusses several uses of diatoms in everyday life and how they are used in science and forensics. Web sites for Additional Information (Web-based information sources) More sites on minerals This Web site, the RRUFF Project, comes from Professor Robert Downs at the University of Arizona. It is an extension of the IMA database of minerals, having the same format, and is a database of the names and composition of more than 3700 of the 4858 IMA minerals, their photos, x-ray data and Raman spectra:  HYPERLINK "http://rruff.info/" http://rruff.info/. One can search by mineral name, their chemical content; e.g., those containing silicon, or specific lack of content; e.g., those with no silicon. Click the Lookup box to the right of the mineral name, and a pull-down menu appears containing all the names of the minerals, from which you can choose the mineral you want, so you dont have to type it in. Click the Lookup box to the right of the Chemistry Includes box and a pull-down periodic table appears, from which you can choose to include or exclude specific elements. This pdf file from the USGS is a poster showing uses of Minerals in Our Environment:  HYPERLINK "http://geopubs.wr.usgs.gov/open-file/of00-144/of00-144.pdf" http://geopubs.wr.usgs.gov/open-file/of00-144/of00-144.pdf. It shows four different rooms in our home and where minerals are found within the contents of that room. If students would like to learn more about crystal structure within minerals, they can visit this site:  HYPERLINK "http://webmineral.com/crystall.shtml" http://webmineral.com/crystall.shtml. It has illustrations of all seven crystal forms, as well as amorphous structures, and their crystal axes. It also contains templates to print out, from which paper models can be made. This HyperPhysics Web page contains a definition of a mineral and an alphabetically-listed table of more than 450 minerals, with photographs of each:  HYPERLINK "http://hyperphysics.phy-astr.gsu.edu/hbase/geophys/mineral.html#c1" http://hyperphysics.phy-astr.gsu.edu/hbase/geophys/mineral.html#c1. More sites on gemstones This site, Crystallography of Gemstones: The Crystal System discusses the seven basic crystal systems found in gemstones:  HYPERLINK "http://www.allaboutgemstones.com/crystalline_structures.html" http://www.allaboutgemstones.com/crystalline_structures.html. It covers the crystal systems in sufficient detail that the site could be used in a study of crystals in high school chemistry. This page from Your Gemologist also discusses crystal types. It could be used to complement the link shown above, as this page doesnt go into as much detail about the crystal structures, but it does show a photo of a crystal that matches each of the types of crystals discussed above (which the link above does not do). ( HYPERLINK "http://www.yourgemologist.com/crystalsystems.html" http://www.yourgemologist.com/crystalsystems.html) More sites on opal(s) This site includes much detailed information about the structures and crystal forms of the various polymorphs of quartz, especially opal:  HYPERLINK "http://www.quartzpage.de/gen_mod.html#opal" http://www.quartzpage.de/gen_mod.html#opal. The topic is opal, not opalsthe page does not have many photos of opals. The Wikipedia article on opal found here,  HYPERLINK "http://en.wikipedia.org/wiki/Opal" http://en.wikipedia.org/wiki/Opal, has good background information about opals, as well as myriad photos of precious opals. Basic information about opals from the viewpoint of a gemologist can be found here:  HYPERLINK "http://www.opal33.com/about-opal.html" http://www.opal33.com/about-opal.html. This Web page from the Gemological Institute of America (GIA) describes the essential factors affecting the value of an opalcolor, pattern and clarityand provides nice photos of a variety of gem-quality opals:  HYPERLINK "http://www.gia.edu/opal-quality-factor" http://www.gia.edu/opal-quality-factor. This page from GIA provides a few facts about and more pictures of opals:  HYPERLINK "http://www.gia.edu/opal" http://www.gia.edu/opal. This page shows a table containing photos of opals, organized by color:  HYPERLINK "http://www.gemdat.org/gem-6666.html" http://www.gemdat.org/gem-6666.html. This is the feature article (pp 2435) from the Summer 2011 issue of InColor magazine. Entitled Wollo Opals: A Powerful Source from Ethiopia, it discusses in detail the history, geography and geology of the opals of the Wollo Province area in Ethiopia. It includes maps of the area and is replete with beautiful, very high-resolution photos of opals found there. ( HYPERLINK "http://www.gemstone.org/webincolor/Summer2011/index.html?pageNumber=24" http://www.gemstone.org/webincolor/Summer2011/index.html?pageNumber=24) You page through the online magazine as if you were reading the hard copy version. For a light-hearted look at opal, check out the American Chemical Societys Chemical & Engineering News article on opal, from their series of articles Whats That Stuff? about common everyday items involving chemistry. ( HYPERLINK "http://pubs.acs.org/cen/whatstuff/stuff/8104sci3.html" http://pubs.acs.org/cen/whatstuff/stuff/8104sci3.html) This blog site from a staff member of the Natural History Museum of Los Angeles County has some exquisite photos of Ethiopan opals:  HYPERLINK "http://nhminsci.blogspot.com/2012/07/loving-ethiopian-opals.html" http://nhminsci.blogspot.com/2012/07/loving-ethiopian-opals.html. The Opal Hut Web site contains information on the formation of opals in, and the geology of, Queensland, Australia:  HYPERLINK "http://www.opalhut.com.au/ausopals/opal.htm#Patterns" http://www.opalhut.com.au/ausopals/opal.htm#Patterns. This site provides a pdf of an article about opals at the micro- and nano-scale levels. It was published in the journal American Mineralogist, written by Eloise Gaillou (et al), the scientist mentioned in the ChemMatters article:  HYPERLINK "http://mineralsciences.si.edu/staff/pages/gaillou/Gaillou-etal_2008_CommonOpalStruc.pdf" http://mineralsciences.si.edu/staff/pages/gaillou/Gaillou-etal_2008_CommonOpalStruc.pdf. To really appreciate the play of color in opals, you have to see the stone in motion. For students who have never seen opals play of color, the following video clips with close-up views of opals may help them understand why opal is so sought-after. 1:30 video clip shows a white opal, 33 mm x 17 mm x 8 mm (1.3 x 0.6 x 0.3 inches):  HYPERLINK "http://www.youtube.com/watch?v=YIOktuNdHWQ" http://www.youtube.com/watch?v=YIOktuNdHWQ 0:47 video clip showing a small lab-made opal with great play of color:  HYPERLINK "http://www.youtube.com/watch?feature=player_embedded&v=RCEiCpp1-ak" http://www.youtube.com/watch?feature=player_embedded&v=RCEiCpp1-ak 4-minute video showing a log that has been infused with opal. The log appears to be about 2 feet long and about 3-1/2 inches in diameter. Lots of play of color here:  HYPERLINK "http://www.youtube.com/watch?v=OrOzVKSftvE" http://www.youtube.com/watch?v=OrOzVKSftvE 1:42 video clip of a boulder opalagain, beautiful play of color:  HYPERLINK "http://www.youtube.com/watch?feature=player_detailpage&v=oWvg0heUioM" http://www.youtube.com/watch?feature=player_detailpage&v=oWvg0heUioM 1:15 video clip of red fire black opal:  HYPERLINK "http://www.youtube.com/watch?v=yvhnKwZgdUI" http://www.youtube.com/watch?v=yvhnKwZgdUI 6-minute video clip of a variety of black opals from Lightning Ridge Opal with lots of play of color:  HYPERLINK "http://www.youtube.com/watch?v=Au4UUlc3BFM" http://www.youtube.com/watch?v=Au4UUlc3BFM 0:56 video clip of a blue fire black opal:  HYPERLINK "http://www.youtube.com/watch?v=VzjHsqxgtos&feature=player_embedded" http://www.youtube.com/watch?v=VzjHsqxgtos&feature=player_embedded 0:34 video clip showing rare fern pattern opal:  HYPERLINK "http://www.youtube.com/watch?v=MVb_a3InQ3Q" http://www.youtube.com/watch?v=MVb_a3InQ3Q 2-minute YouTube video clip showing some interesting play of color among white opals; it also provides very basic science behind the phenomenon:  HYPERLINK "http://www.youtube.com/watch?feature=player_embedded&v=wrjGANFmftk" http://www.youtube.com/watch?feature=player_embedded&v=wrjGANFmftk. And this 10-minute YouTube video clip discussing the Royal Peacock Opal Mine in Virgin Valley, Nevada. It is a site for public digging for opals (for a fee, of course), and it shows prospectors digging (and of course, finding) opals in the clay banks. ( HYPERLINK "http://www.bing.com/videos/search?q=opal&qs=VI&form=QBVR&pq=opal&sc=8-4&sp=3&sk=HS2#view=detail&mid=EE0F3F6014A7C118ABD0EE0F3F6014A7C118ABD0" http://www.bing.com/videos/search?q=opal&qs=VI&form=QBVR&pq=opal&sc=8-4&sp=3&sk=HS2#view=detail&mid=EE0F3F6014A7C118ABD0EE0F3F6014A7C118ABD0) More sites on silica and silicates This site provides a nice overview of the various types of linkages in silicate structures:  HYPERLINK "http://www.tulane.edu/~sanelson/eens211/silicate_structures08.htm" http://www.tulane.edu/~sanelson/eens211/silicate_structures08.htm. The Mineral Gallery Web site at  HYPERLINK "http://galleries.com/Silicates" http://galleries.com/Silicates contains a list of many of the silicate minerals and the type of tetrahedral structure (one of six) they exhibit. The site also contains descriptions and photos of each of the minerals. The Vision Learning site has a page with illustrations of each of the six types of silicates. Each illustration has the ball and stick model as well as a tetrahedron model, and each type has an animation to show students what they look like in 3-D. ( HYPERLINK "http://web.visionlearning.com/silica_molecules.shtml" http://web.visionlearning.com/silica_molecules.shtml) More sites on quartz This site contains an extensive group of photos (>200), in table format, of quartz gemstones, organized according to color:  HYPERLINK "http://www.gemdat.org/gem-3337.html" http://www.gemdat.org/gem-3337.html. The extensive Quartz Page Web site provides very in-depth coverage of the mineral quartz. This page details the structure of quartz, providing nice atomic-level models, including polyhedra views of the various structures:  HYPERLINK "http://www.quartzpage.de/gen_struct.html" http://www.quartzpage.de/gen_struct.html. A companion page provides great detail about the different polymorphs of silica (including opal) and includes a phase diagram that includes many of the silica polymorphs:  HYPERLINK "http://www.quartzpage.de/gen_mod.html#opal" http://www.quartzpage.de/gen_mod.html#opal. The site includes tables of physical properties, chemical properties, occurrence and crystallographic data about the silica polymorphs The Chemical Properties page provides chemical reactions quartz typically undergoes:  HYPERLINK "http://www.quartzpage.de/gen_chem.html" http://www.quartzpage.de/gen_chem.html Physical Properties:  HYPERLINK "http://www.quartzpage.de/gen_phys.html" http://www.quartzpage.de/gen_phys.html The following VRML images are from the jcrystal.com Web site. They show the atomic-level structures of quartz polymorphs using Cortona 3-D to provide an interactive structure, allowing you to control its movement. It shows a ball-and stick model with the polyhedra superimposed over the structure. As you rotate the structure you can see the polyhedra linked at their corners. The software is not user-friendly, as the image opens up huge, with one bond filling the entire screen; to decrease the size, hit the farthermost button on the right of the tool bar at the bottom, the full-size screen button. This makes the entire structure fit the screen. Then you can rotate the entire structure to view its various features. The box shape in each structure is the unit cell for that structure. This image is quartz:  HYPERLINK "http://jcrystal.com/steffenweber/gallery/StructureTypes/quartz.wrl" http://jcrystal.com/steffenweber/gallery/StructureTypes/quartz.wrl. Here is the same VRML image, but of cristobalite, one of the polymorphs of quartz:  HYPERLINK "http://jcrystal.com/steffenweber/gallery/StructureTypes/cristobalite.wrl" http://jcrystal.com/steffenweber/gallery/StructureTypes/cristobalite.wrl. Here is the same VRML image, but of tridymite, another of the polymorphs of quartz:  HYPERLINK "http://jcrystal.com/steffenweber/gallery/StructureTypes/tridymite.wrl" http://jcrystal.com/steffenweber/gallery/StructureTypes/tridymite.wrl. More sites on diatoms This short online paper (23 pages, depending on screen size) from Ken Perreault of St. Michaels College discusses in fair detail the nature and importance of diatoms to our world:  HYPERLINK "http://academics.smcvt.edu/dfacey/AquaticBiology/Freshwater%20Pages/Diatoms.html" http://academics.smcvt.edu/dfacey/AquaticBiology/Freshwater%20Pages/Diatoms.html. This online article is from PLoS Biology, an online, open-access, peer-reviewed publication. It describes potential uses for production of electronic components mimicking the nano-scale synthesis techniques that diatoms utilize to build their silica shells. ( HYPERLINK "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC521728/" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC521728/) This Web page created by David Mann, from the Tree of Life Web Project, discusses at length what diatoms are and how they affect life on Earth:  HYPERLINK "http://tolweb.org/Diatoms/21810" http://tolweb.org/Diatoms/21810. This page from Henderson State University shows pictures of many different types of diatoms and includes a photo of a glassy shell:  HYPERLINK "http://www.hsu.edu/pictures.aspx?id=1954" http://www.hsu.edu/pictures.aspx?id=1954. Heres one more site that features research on diatoms to learn how they synthesize their silica shells so scientists and engineers can duplicate this feat to design nanoscale electronic devices. Here are a few Web sites from Science Daily discussing diatoms. Each site focuses on one use of diatoms: Biosensors with fluorescent proteins:  HYPERLINK "http://www.sciencedaily.com/releases/2012/03/120323094036.htm" http://www.sciencedaily.com/releases/2012/03/120323094036.htm Monitoring water quality using diatoms:  HYPERLINK "http://www.sciencedaily.com/releases/2010/05/100504095111.htm" http://www.sciencedaily.com/releases/2010/05/100504095111.htm Extracting oil from diatoms to solve the energy crisis:  HYPERLINK "http://www.sciencedaily.com/releases/2009/06/090622165830.htm" http://www.sciencedaily.com/releases/2009/06/090622165830.htm Using diatoms to reveal past climate changes:  HYPERLINK "http://www.sciencedaily.com/releases/2009/10/091029151621.htm" http://www.sciencedaily.com/releases/2009/10/091029151621.htm Diatoms sequester CO2 from oceans:  HYPERLINK "http://www.sciencedaily.com/releases/2009/03/090317125217.htm" http://www.sciencedaily.com/releases/2009/03/090317125217.htm Heres the text of the 1979 National Geographic article Those Marvelous, Myriad Diatoms:  HYPERLINK "http://howtousediatomaceousearth.com/wp-content/uploads/2010/12/MarvelousMyriadDiatoms.pdf" http://howtousediatomaceousearth.com/wp-content/uploads/2010/12/MarvelousMyriadDiatoms.pdf This is a 1:14 video clip that shows several different types of diatoms under a microscope moving across the field of vision:  HYPERLINK "http://wn.com/diatom_shells#/videos" http://wn.com/diatom_shells#/videos. This Web site on diatoms has many different pages and lots of good photos:  HYPERLINK "http://earthguide.ucsd.edu/diatom/d1.html" http://earthguide.ucsd.edu/diatom/d1.html. Each page takes the student through a different aspect of diatoms, from morphology to habitat, ecology, and fossils. The information is still valid, but be aware that most of the reference links are no longer valid links (copyrighted 1999). More sites on Earths structure This page from the HyperPhysics Web site provides a very brief introduction to the structure of the Earth:  HYPERLINK "http://hyperphysics.phy-astr.gsu.edu/hbase/geophys/earthstruct.html#c1" http://hyperphysics.phy-astr.gsu.edu/hbase/geophys/earthstruct.html#c1. Here is another basic discussion of the structure of the Earth, with simple animations, from the USGS:  HYPERLINK "http://www.scec.org/education/k12/learn/plate1.htm" http://www.scec.org/education/k12/learn/plate1.htm. This series of seven slides shows the structure of the Earth:  HYPERLINK "http://education.nationalgeographic.com/education/encyclopedia/core/?ar_a=1" http://education.nationalgeographic.com/education/encyclopedia/core/?ar_a=1. The series includes slide 6, this downloadable pdf file poster that contains 5 of the seven slides on one sheet:  HYPERLINK "http://education.nationalgeographic.com/media/file/Earth_Interior_Poster.pdf" http://education.nationalgeographic.com/media/file/Earth_Interior_Poster.pdf. More sites on polymer opal films This is the actual Rainbow Winters site, from which the 2012 Paris Fashion Week photo used in the ChemMatters article was taken:  HYPERLINK "http://www.rainbowwinters.com/project6.html" http://www.rainbowwinters.com/project6.html. There is a short gallery of the Liquid Bodysuit: stretch reactive clothes. This <1-minute video shows the stretching and relaxing of a polymer opal film, from the University of Cambridge:  HYPERLINK "http://vk.com/video-23304496_165270070" http://vk.com/video-23304496_165270070. This site from the NanoPhotonics Center of the University of Cambridge provides 5 very brief video clips of different polymer opal films being bent and stretched:  HYPERLINK "http://www.np.phy.cam.ac.uk/research-themes/polymer-opal-videos" http://www.np.phy.cam.ac.uk/research-themes/polymer-opal-videos. More sites on photonic crystals This article is a pdf of the original December 2001 Scientific American article on Photonic Crystals: Conductors of Light by Eli Yablonovitch (pp 4655):  HYPERLINK "http://optoelectronics.eecs.berkeley.edu/eliy_SCIAM.pdf" http://optoelectronics.eecs.berkeley.edu/eliy_SCIAM.pdf. More sites on structural color COLR Technologys Web site briefly describes their process of using iron oxide superparamagnetic Colloidal Nanocrystal Clusters (CNCs) to make individual particles self-assemble to form a microscopic, lattice-like structure which diffracts specific wavelengths of light. Adjusting the strength of the magnetic field tunes the color to display brilliant, iridescent colors across the entire visible spectrum and beyond. ( HYPERLINK "http://colrtechnology.com/index.php" http://colrtechnology.com/index.php) More Web sites on Teacher Information and Lesson Plans (sites geared specifically to teachers) The Princeton University Press book Photonic Crystals: Molding the Flow of Light, 2nd edition (copyright 2007) is available free in pdf form (13 MB) at  HYPERLINK "http://ab-initio.mit.edu/book/photonic-crystals-book.pdf" http://ab-initio.mit.edu/book/photonic-crystals-book.pdf. Note: the book is very advanced, as youd expect. And here is a very extensive PowerPoint (35 MB) on photonic crystals from the same source as above:  HYPERLINK "http://ab-initio.mit.edu/photons/tutorial/leos-course.ppt" http://ab-initio.mit.edu/photons/tutorial/leos-course.ppt. Although most of the math is way beyond anything in high school chemistry or math classes, there are a lot of great photos at the micrometer level that you could use in your classroom. In addition to the two documents referenced above, other materials from Dr. Steve Johnsons 2003 lectures on photonic crystals at MIT can be obtained at this site:  HYPERLINK "http://ab-initio.mit.edu/photons/tutorial/" http://ab-initio.mit.edu/photons/tutorial/. General Web References (Web information not solely related to article topic) Jmol is an open-source Java viewer for chemical structures in 3-D. It is free downloadable software that allows you to show atomic-level illustrations of structures of many compounds. These structures are rotatable and zoomable, and some have animation, with vibration and rotation capabilities. It is an excellent source for molecular visualizations for students. ( HYPERLINK "http://jmol.sourceforge.net/" http://jmol.sourceforge.net/) The Virtual Museum of Minerals and Molecules at  HYPERLINK "http://virtual-museum.soils.wisc.edu/" http://virtual-museum.soils.wisc.edu/ is a site that provides visualizations of molecular and macromolecular structures using Jmol (cited above). Its focus is on soil minerals and organic compounds. It is ideal for showing students the actual structure of a group of molecules; quartzs tetrahedra or graphite vs. diamond.      PAGE \* MERGEFORMAT 26  PAGE \* MERGEFORMAT 1 PAGE  PAGE 131 (from HYPERLINK "http://www.esrl.noaa.gov/gmd/ccgg/trends/" http://www.esrl.noaa.gov/gmd/ccgg/trends/) ( HYPERLINK "http://epa.gov/climatechange/ghgemissions/gases/co2.html" http://epa.gov/climatechange/ghgemissions/gases/co2.html) U.S. Carbon Dioxide Emissions, By Source U.S. Methane Emissions, By Source (from  HYPERLINK "http://epa.gov/climatechange/ghgemissions/gases/ch4.html" http://epa.gov/climatechange/ghgemissions/gases/ch4.html) (from  HYPERLINK "http://eo.ucar.edu/kids/green/cycles6.htm" http://eo.ucar.edu/kids/green/cycles6.htm) ( HYPERLINK "http://www.epa.gov/climatechange/ccs/" http://www.epa.gov/climatechange/ccs/) The references below can be found on the ChemMatters 30-year CD (which includes all articles published during the years 1983 through April 2013 and all available Teachers Guides). The CD is in production and will be available from the American Chemical Society at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://ww.acs.org/chemmatters. Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online on the same site. (The complete set of ChemMatters articles and Teachers Guides are available on the 30-year CD for all past issues, (up to April 2013.) Some of the more recent articles (2002 forward) may also be available online at the link above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the Web page. If the article is available online, you will find it there. 30 Years of ChemMatters ! The references below can be found on the ChemMatters 30-year CD (which includes all articles published during the years 1983 through April 2013 and all available Teachers Guides). The CD is in production and will be available from the American Chemical Society at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://ww.acs.org/chemmatters. Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online on the same site. (The complete set of ChemMatters articles and Teachers Guides are available on the 30-year CD for all past issues, (up to April 2013.) Some of the more recent articles (2002 forward) may also be available online at the link above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the Web page. If the article is available online, you will find it there. 30 Years of ChemMatters ! (from  HYPERLINK "http://en.wikipedia.org/wiki/Nucleus_accumbens" http://en.wikipedia.org/wiki/Nucleus_accumbens) The references below can be found on the ChemMatters 30-year CD (which includes all articles published during the years 1983 through April 2013 and all available Teachers Guides). The CD is in production and will be available from the American Chemical Society at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://ww.acs.org/chemmatters. Selected articles and the complete set of Teachers Guides for all issues from the past three years are also available free online on the same site. (The complete set of ChemMatters articles and Teachers Guides are available on the 30-year CD for all past issues, (up to April 2013.) Some of the more recent articles (2002 forward) may also be available online at the link above. Simply click on the Past Issues button directly below the M in the ChemMatters logo at the top of the Web page. If the article is available online, you will find it there. 30 Years of ChemMatters ! Order of Weight % Molar % Volume % Abundance Element in crust in crust in crust Typical natural form at Earth Surface 1 Oxygen 46.3 60.2 94.2 O2- In minerals and H2O; small amount as elemental O2 in atmosphere 2 Silicon 28.2 20.8 0.8 Almost all as Si4+ in silicate minerals; some as H4SiO4 in seawater 3 Aluminum 8.1 6.2 0.4 Almost all as Al3+ in minerals 4 Hydrogen 0.1 2.9 - Almost all as H+ in H2O, OH in Minerals and HCO31- 5 Sodium 2.4 2.2 1.1 All as Na+, largely in minerals but also in seawater 6 Calcium 4.1 2.1 1.2 All as Ca2+, largely in minerals but also in seawater 7 Iron 5.4 2.0 0.4 Mostly as Fe2+ and Fe3+ in minerals 8 Magnesium 2.3 2.0 0.3 All as Mg2+, largely in minerals but also in seawater 9 Potassium 2.1 1.1 1.5 All as K+, largely in minerals but also in seawater 10 Titanium 0.5 0.2 0.04 Almost all as Ti4+ in minerals 11 Phosphorus 0.1 0.1 0.002 Mostly as P5+ in phosphate (PO43-) 12 Fluorine 0.06 0.07 0.1 All as F1-, largely in minerals but also in seawater 13 Carbon 0.02 0.04 0.0003 Range of valence states from 4- to 4+ 14 Manganese 0.1 0.04 0.007 Mostly as Mn2+, Mn3+, and Mn4+ in minerals 15 Sulfur 0.03 0.02 0.004 Almost all as S6+ in sulfate (SO42-) or S2- in sulfides . . .    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The CD is in production and will be available from the American Chemical Society at this site:  HYPERLINK "http://www.acs.org/chemmatters" http://ww.acs.org/chemmatters. Selected articles and the complete set of Teacher s Guides for all issues from the past three years are also available free online on the same site. (The complete set of ChemMatters articles and Teacher s Guides are available on the 30-year CD for all past issues, (up to April 2013.) Some of the more recent articles (2002 forward) may also be available online at the link above. Simply click on the  Past Issues button directly below the  M in the ChemMatters logo at the top of the Web page. If the article is available online, you will find it there. 30 Years of ChemMatters ! 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