ࡱ> ,.+Y bjbjWW ==] ?((PPPPPPP$ *PPPPP*`PP(```PPPP``P }Q)jConverting mass of a DNA band into moles: First, decide if the band is composed of single or double-stranded DNA. Then, chose the correct conversion factor: Hyseq uses *: Single-stranded: 330 grams per mole per base 330 (g/mol)/b 330 g / ( mol ( b ) Double-stranded: 660 grams per mole per base pair 660 (g/mol)/bp 660 g / ( mol ( bp ) Example problem: Find the number of moles which compose a 1.5kb band whose mass was determined to be 120 ng (by gel comparison to standards). (120 n g ) ( ( mol ( bp ) = 1.2E-4 nmol or 0.12 pmol (1500 bp) ( 660 g ) *Other sources use slightly different factors: 323 for single and 610 for double; see:  HYPERLINK http://www.cbs.dtu.dk/nikob/science/nuc_table.html http://www.cbs.dtu.dk/nikob/science/nuc_table.html To be truly accurate, determine ratio of each base then calculate conversion factor; see:  HYPERLINK http://www.idtdna.com/html/tech/molwt.html http://www.idtdna.com/html/tech/molwt.html )*`a :<KMSz{jAU0JjU jUCJ7CJ j>*CJ 7>*CJ >*CJ jCJ CJ CJ4CJ0(*+st;Nghiz{4QRS z*+st;Nghiz{4QRS z / =!"#$%ADyK 3http://www.cbs.dtu.dk/nikob/science/nuc_table.htmlyK fhttp://www.cbs.dtu.dk/nikob/science/nuc_table.html!DyK +http://www.idtdna.com/html/tech/molwt.htmlyK Vhttp://www.idtdna.com/html/tech/molwt.html [$@$NormalmH <A@<Default Paragraph Font*B`* Body TextCJ(U@( Hyperlink>*B*   zXXFIKM\_bd !%/3:<Z_ CJ todd desantisEC:\DOCUME~1\TODDDE~1\LOCALS~1\Temp\AutoRecovery save of Document3.asd todd desantisBC:\My Documents\Hyseq\Converting mass of a DNA band into moles.doc todd desantisdC:\DOCUME~1\TODDDE~1\LOCALS~1\Temp\AutoRecovery save of Converting mass of a DNA band into moles.asd todd desantisBC:\My Documents\Hyseq\Converting mass of a DNA band into moles.doc todd desantisBC:\My Documents\Hyseq\Converting mass of a DNA band into moles.doc todd desantisBC:\My Documents\Hyseq\Converting mass of a DNA band into moles.doc todd desantisdC:\DOCUME~1\TODDDE~1\LOCALS~1\Temp\AutoRecovery save of Converting mass of a DNA band into moles.asd todd desantisBC:\My Documents\Hyseq\Converting mass of a DNA band into moles.doc@s@GzTimes New Roman5Symbol3& zArial"qhhIII/3!20d)Converting mass of a DNA band into moles: todd desantis todd desantisOh+'0  $0 L X d p|*Converting mass of a DNA band into moles:rdonvtodd desantiss oddNormalstodd desantiss 5ddMicrosoft Word 8.0a@ِ@zP)@P7I)@ۘQ)3՜.+,D՜.+,X hp  BBRPwj *Converting mass of a DNA band into moles: Title(RZ _PID_GUID _PID_HLINKSAN{CBC488C6-79FB-49C3-B99A-A3426CA2BD56}A, h8+http://www.idtdna.com/html/tech/molwt.html 3http://www.cbs.dtu.dk/nikob/science/nuc_table.html  !"$%&'()*-Root Entry F fqN)cQ)/Data  1TableWordDocumentSummaryInformation(DocumentSummaryInformation8#CompObjjObjectPoolcQ)cQ)  FMicrosoft Word Document MSWordDocWord.Document.89q