ࡱ> 79456g mbjbjVV 3pr<r<x& 8N߾"VO$s  $ol0i&A&A&A0___&A~_&A __.H0e*R0 D@x0߾\Ա )_I25900r^߾&A&A&A&A : CURRICULUM VITAE James D. Fluckey, Ph.D. Muscle Biology Laboratory Department of Health and Kinseiology Texas A&M University Old Heep Bld, Room 219A Mail Stop 4243 College Station, TX 77843 Office Phone: 979-458-2061  HYPERLINK "mailto:jfluckey@hlkn.tamu.edu" jfluckey@hlkn.tamu.edu Current Status Assistant Professor in the Department of Health and Kinesiology A note about this CV: There was an abundance of work conducted at other institutions prior to arriving at Texas A&M University. In fact, my arrival to Texas A&M University occured one month prior to becoming a tenured Associate Professor at the University of Arkansas for Medical Sciences. Since I am only in my third year at Texas A&M University, I have highlighted in maroon achievements or additions that have occurred since my arrival. Current Research Interests Primarily focused on mechanisms associated with protein turnover and glucoregulatory function in muscle, and how these mechanisms may be altered by exercise, aging, obesity/diabetes, and/or periods of microgravity. Current studies specifically focus on signal transduction mechanisms involved with muscle protein synthesis and degradation using robust activation/blockade methodologies. Other current studies focus on muscle protein metabolism during conditions of acclerated muscle atrophy, such as simulated microgravity. Experiments range from systems to cellular/molecular levels in both humans and animals using both basic and applied approaches. Laboratory Dr. Fluckeys research is conducted in the Muscle Biology Laboratory, Old Heep Bldg, Room 219. Muscle Biology Laboratory The Muscle Biology Laboratory was established in 1992, under the direction of Robert B. Armstrong. This laboratory specifically focuses on skeletal muscle biology and physiology, as well as how muscle adapts to exercise, inactivity and aging. Educational Background August 1992 to July 1995 - Attended The Pennsylvania State University. Major: Physiology Mentor: Peter A. Farrell, Professor Predoctoral Fellow in the Noll Physiological Research Center Sponsor: National Institute on Aging - Gerontology December 1995 - Doctorate of Philosophy degree in Physiology (3.77 GPA). August 1990 to May 1992 - Attended Ball State University. Major: Exercise Physiology Mentor: Bruce W. Craig, Ph.D. Graduate Assistant in the Human Performance Laboratory May 1992 - Master of Science degree in Exercise Physiology (3.81 GPA). September 1988 to May 1990 - Attended Eastern New Mexico University. Major: Health and Physical Education Advisor: Julie Wood, Ph.D. Minor: Biology. May 1990 - Bachelor of Science degree in Health and Physical Education (3.99 GPA). January 1986 to May 1989 - Attended Eastern New Mexico University - Clovis Campus. The campus is now known as Clovis Community College. Major: Health and Fitness Technology. Advisor: John OConnell, M.S. May 1989 - Associate of Applied Science degree in Health and Fitness Technology (4.00 GPA). May 1976 - Graduated from Clovis High School, Clovis, NM. Professional Experience: 2009- present Associate Professor in the Department of Health and Kinesiology, Muscle Biology Laboratory, Texas A&M University 2005 2009 Assistant Professor in the Department of Health and Kinesiology and Muscle Biology Laboratory, Texas A&M University 2005 Awarded Promotion and Tenure at the University of Arkansas for Medical Sciences (was to be effective July 1, 2005). 1999 2002 Director of the Metabolic Analyses Core in the General Clinical Research Center of the University of Arkansas for Medical Sciences 1998 2005 Assistant Professor in the Department of Geriatrics at the University of Arkansas for Medical Sciences, Nutrition, Metabolism and Exercise Laboratory William J. Evans, Ph.D., Director 1997 Part-time Instructor in the Department of Biology at Pitt Community College, Greenville, NC 1997 Research Associate in the Department of Biochemistry at East Carolina University School of Medicine, Greenville, NC G. Lynis Dohm, Ph.D., Mentor Associate Professor in the Department of Medical Physiology at the University of Copenhagen (academic rank was given to accommodate funded salary support through a Danvis Research Grant) 1995-1996 Assistant Professor in the Department of Medical Physiology at the University of Copenhagen (academic rank was given to accommodate funded salary support through a Danvis Research Grant) J. William Fulbright Scholar in the Department of Medical Physiology at the University of Copenhagen and Copenhagen Muscle Research Centre, Copenhagen, Denmark Henrik Galbo, Professor, Mentor NIA-Predoctoral Fellow at the Noll Physiological Research Center at The Pennsylvania State University, University Park, PA Peter A. Farrell, Ph.D., Mentor/Advisor Graduate Research Assistant in the Human Performance Laboratory of Ball State University, Muncie, IN Bruce Craig, Ph.D., Advisor 1987-1990 Part-time Instructor at Eastern New Mexico University - Clovis Campus (now called the Clovis Community College). 1973-1986 Home builder for Custom Woodworks, Inc., 1600 E. 2nd St., Clovis, NM 88101. Honors and Awards: Exercise and Sports Science Reviews, Assistant Editor 2009 to present. Invited Interview (television) with MBC - Korea (Munhwa Broadcasting Corporation) Anabolic Steriod Abuse among Teenagers October 15, 2007. American Journal of Physiology: Endocrinology and Metabolism, Editorial Board Member 2007 to present. Listed as a STAR reviewer for Physiological Genomics and the American Physiological Society, Washington D.C. EB April 28, 2007. Recipient of NIH Loan Repayment Program Award, 2004- 2005. Featured in Geriatrics Spotlight article at UAMS, 2003. Recipient of NIH Loan Repayment Program Award, 2002-2004. Chair of the Carbohydrate and Fat Metabolism and Aging session at the 11th International Conference on the Biochemistry of Exercise (2000). Co-Organizer of the 11th International Conference on the Biochemistry of Exercise: Little Rock, AR, June 4-7, 2000. Selected as an International Whos Who of Professionals (1997). Recipient of a Research Grant from the Copenhagen Muscle Research Centre to explore the actions of insulin on glucose and muscle protein metabolism following resistance exercise (1996). Recipient of a Research Grant from the Danish Research Academy (DANVIS Grant) for 1996. As a result of this grant, I was made a research faculty member in the Department of Medical Physiology of Copenhagen University. Recipient of a Congressional J. William Fulbright Fellowship to conduct research at the PANUM Institute and Copenhagen Muscle Research Center, University of Copenhagen, Denmark (1995-1996). Honorable Mention in the Graduate Research Exhibition at The Pennnsylvania State University (1995). Recipient of NIA Predoctoral Grant to complete doctoral research entitled Insulin Modulation of Muscle Protein Synthesis after Resistance Exercise (1994-1995). Selected as a Gerontology Predoctoral Fellow in Physiology at The Pennsylvania State University from 1992 to 1995 (National Research Award from the National Institute of Aging). Selected to Outstanding College Students of America for the 1989 - 1990 academic year. Selected as the Outstanding Men's Undergraduate in Health and Physical Education at ENMU for 1990. Selected as NASPE Outstanding Physical Education Major from the state of New Mexico in 1990. Selected as the Outstanding Men's Undergraduate in Health and Physical Education at ENMU for 1989. Peer Reviewer (grants): Ad hoc grant reviewer for a Claude Pepper Center Pilot Grant, University of Michigan (2008) Ad hoc grant reviewer for the Chief Scientist Office, Scottish Executive Government (2006) External mock grant reviewer for a Pepper Center, University of Texas, Medical Branch (Reviewed 2 separate grants at the request of UTMB, 2004) Ad hoc grant reviewer for the Veterans Administration (VA Merit Grant; 2003) Ad hoc grant reviewer for the Nova Scotia Research Foundation (2001) Ad hoc grant reviewer for the National Science Foundation (2000) Peer Reviewer (manuscripts): Manuscript reviewer for: American Journals of Physiology Journal of Applied Physiology Journal of Nutrition Journal of Physiology Medicine and Science in Sports and Exercise International Journal of Sports Medicine Canadian Journal of Applied Physiology Physiological Genomics Muscle and Nerve (Maroon indicates new Journals since arriving to TAMU; Other Journals listed still frequently use my review services.) Peer Reviewer (text book): Reviewer for Lippincott Publishing Company: Exercise Physiology Text Book (Authors Kraemer and Fleck) Past and Current Committees (UAMS/VA and Texas A&M University): 2010 Texas A&M University Institutional Review Board #1, Chairman 2008 Space Life Sciences Undergraduate Scholarship Committee 2008 Faculty Search Committee, Department of Health and Kinesiology, Texas A&M University Huffines Director/Senior Scientist 2008 - Member of the Institutional Animal Care and Use Committee 2007 Member of the Texas A&M University Institutional Review Board 2005-2006 Faculty Search Committee, Department of Health and Kinesiology, Texas A&M University 2003-2005 Member of the Institutional Animal Care and Use Committee (IACUC) at UAMS 2002 Faculty Search Committee, Department of Geriatrics, UAMS 2002- 2005 Member of the Research Safety Subcommittee in the Arkansas Veterans Administration Health Care System 2000-2005 Member of the GCRC Advisory Committee (GAC) at UAMS Professional Societies: American Physiological Society American College of Sports Medicine Bibliography (Presented in Reverse Chronology): A note on authorship in this field. Although all authors listed on a manuscript or presentation are of great importance in this field, the first author assumes a great deal of ownership/responsibility of the work, and was instrumental in day to day management of the study and primary data collection/assessment/interpretation. Typically, the second author also played a prominent role on the project, but to a lesser degree unless otherwise indicated. In this field, particular emphasis with regard to published works is also placed on the senior (last) author in the list, as this is typically indicative of whos lab the work transpired. In most cases, when students are involved with publications and presentations, the senior author is the students mentor, and is often given equal credit by his/her peers in the field. It is not uncommon for scientists in the field to associate published works on the basis of the senior author, particularly if the work was done in a prominent lab. Thus, it is a natural transition in this field to move from being the first author to senior author as the scientist becomes more prominent in his/her area of expertise. Gasier HG, SE Riechman, MP Wiggs, A Buentello, SF Previs and JD Fluckey. Cumulative responses of muscle protein synthesis are augmented with chronic resistance exercise training. Acta Physiologica, In Press, 2010. Nilsson MI, Greene NP, Dobson JP, Wiggs MP, Gasier HG, Macias BR, Shimkus KL and Fluckey JD. Insulin resistance syndrome blunts the mitochondrial anabolic response following resistance exercise. Am J Physiol Endocrinol Metab 299: E466-E474, 2010. PMID: 20606077 Gasier HG, Fluckey JD and Previs SF. The application of 2H2O to measure skeletal muscle protein synthesis. Nutr Metab (Lond) 7: 31, 2010. PMID: 20409307 Gasier HG, Previs SF, Pohlenz C, Fluckey JD, Gatlin DM 3rd, Buentello JA. A novel approach for assessing protein synthesis in channel catfish, Ictalurus punctatus. Comp Biochem Physiol B Biochem Mol Biol. 2009 Oct;154(2):235-8. Epub 2009 Jun 27. PMID: 19563906 Gasier HG, Riechman SE, Wiggs MP, Previs SF, Fluckey JD. A comparison of 2H2O and phenylalanine flooding dose to investigate muscle protein synthesis with acute exercise in rats. Am J Physiol Endocrinol Metab. 2009 Jul;297(1):E252-9. Epub 2009 Apr 14. PMID: 19366878 Norrbrand L, JD Fluckey, M Pozzo, PA Tesch. Eccentric overload appears necessary to optimize skeletal muscle adaptations to chronic resistance exercise. Eur J Appl Physol 102(3):271-81, 2008. Dupont-Versteegden EE, BA Strotman, CM Gurley, D Gaddy, M Knox, JD Fluckey, and Peterson CA. Nuclear translocation of EndoG at the initiation of disuse muscle atrophy and apoptosis is specific to myonuclei. Am J Physiol Regul Integr Comp Physiol. 291(6):R1730-40, 2006. Dupont-Versteegden EE, JD Fluckey, M Knox, D Gaddy, and CA Peterson. The effect of flywheel-based resistance exercise on processes contributing to muscle atrophy during unloading in adult rats. J. Appl. Physiol. 101:202-12, 2006. Fluckey JD, M Knox, LM Smith, EE Dupont-Versteegden, D Gaddy, PA Tesch, and CA Peterson. The insulin-facilitated increase of muscle protein synthesis after resistance exercise involves a MAP-kinase pathway. Am. J. Physiol - Endocrinol.& Metab. 290:E1205-11, 2006. Hays NP, RD Starling, DH Sullivan, JD Fluckey, RH Coker, and WJ Evans. Comparison of insulin sensitivity assessment indices with euglycemic-hyperinsulinemic clamp data following a dietary and exercise intervention in older adults. Metab.: Clin. and Exp. 55: 525-532, 2006. Hays NP, RD Starling, DH Sullivan, JD Fluckey, RH Coker, RH Williams, JA Carrithers and WJ Evans. Effects of an ad libitum high carbohydrate diet and aerobic exercise training on insulin action and muscle metabolism in older men and women. J. Gerontol.: Biol. Sci. and Med. Sci.: 61A(3):299-304, 2006. Carroll CC, JD Fluckey, RH Williams, DH Sullivan and TA Trappe. Human soleus and vastus lateralis muscle protein metabolism with an amino acid infusion. Am. J. Physiol - Endocrinol.& Metab. 288: E479-E485, 2005. Fluckey JD, EE Dupont-Versteegden, M Knox, D Gaddy, PA Tesch and CA Peterson. Insulin facilitation of muscle protein synthesis following resistance exercise in hindlimb suspended rats is independent of a rapamycin-sensitive pathway. Am. J. Physiol. 287: E1070-E1075, 2004. Campbell WW, MD Haub, JD Fluckey, RE Ostlund, Jr., H Morse-Carrithers, MW Hulver, JP Thyfault, and ZK Birge. Pinitol supplementation and insulin-mediated glucose metabolism in older humans. J. Nutr. 134: 2998-3003, 2004. Fluckey JD, RN Cortright, E Tapscott, T Koves, L Smith, S Pohnert and GL Dohm. Active involvement of PKC for insulin-mediated rates of muscle protein synthesis. Am. J. Physiol. 286: E753-E758, 2004. Hays N P, RD Starling, X Liu, DH Sullivan, TA Trappe, JD Fluckey, and WJ Evans. Effects of an ad libitum, low-fat, high-carbohydrate diet on body weight, body composition, and fat distribution in older men and women: a randomized control trial. Arch. Intern. Med. 164: 210-217, 2004. Knox M, JD Fluckey, CA Peterson, P Bennett, CA Peterson and EE Dupont-Versteegden. Hind limb unloading in adult rats using an alternative tail harness design. Aviat. Space Environ. Med. 75: 692-696, 2004. Trappe S, P Gallagher, M Harber, J Carrithers, J Fluckey and T Trappe. Single muscle fiber contractile properties in young and old men and women. J Physiol, 552.1: 47-58, 2003. Wells AM, MD Haub, JD Fluckey, DK Williams, R Chernoff and WW Campbell. Comparisons of vegetarian and beef-containing diets on hematological indexes and irons stores during a period of resistive training in older men. J. Am. Diet. Assoc. 103:594-601, 2003. Fluckey JD, E Dupont-Versteegden, DC Montague, M Knox, CA Peterson and D Gaddy Kurten. A rat resistance exercise regimen attenuates losses of musckuloskeletal mass during hindlimb suspension. Acta Physiol. Scand. 174: 293-300, 2002. Fluckey JD, S Asp, LH Enevoldsen, and H Galbo. Alterations of insulin-mediated muscle protein synthesis in rats after high intensity eccentric exercise. Acta Physio. Scand. 173: 379-384, 2001. Trappe TA, JD Fluckey, F White, CP Lambert and WJ Evans. Skeletal muscle PGF2a and PGE2 in response to eccentric exercise: Influence of ibuprofen and acetaminophen. J. Clin. Endo. Metab., 86(10): 5067-5070, 2001. Enevoldsen LH, B Stallknecht, JD Fluckey and H Galbo. The effect of exercise training on in vivo epinephrine-stimulated lipolysis in intraabdominal adipose tissue in rats. Am. J. Physiol., 279: E585-E592, 2000. Enevoldsen LH, B Stallknecht, JD Fluckey and H Galbo. The effect of exercise training on in situ insulin-stimulated glucose uptake in intraabdominal adipose tissue in rats. Am. J. Physiol., 278:E25-E34, 2000. Fluckey JD, SC Pohnert, SG Boyd, RN Cortright, TA Trappe and GL Dohm. Insulin stimulation of muscle protein synthesis in obese Zucker rats is not via a rapamycin-sensitive pathway. Am. J. Physiol. 279: E182-E187, 2000. Hernandez JM, T Moccia, JD Fluckey, J S Ulbrecht and PA Farrell. Fluid snacks to help persons with insulin-dependent diabetes mellitus avoid late onset post-exercise hypoglycemia. Med. Sci. Sports Exerc. 32: 904-910, 2000. Fluckey JD, T Ploug and H Galbo. Attenuated insulin action on glucose uptake and transport following resistance exercise in rats. Acta Physiol. Scand. 167: 77-82, 1999. Fluckey JD, T Ploug and H Galbo. Mechanisms associated with hypoxia- and contraction-mediated glucose transport are fiber-type dependent. Acta Physiol. Scand. 167: 83-87, 1999. Cortright RN, D Zheng, JP Jones, JD Fluckey, BB Lowell and GL Dohm. The effect of denervation and exercise on UCP-3 gene expression in skeletal muscle. Am. J. Physiol. 276: E217-E221, 1999. Farrell PA, MJ Fedele, J Hernandez, JD Fluckey, JL Miller III, TC Vary, SR Kimball and LS Jefferson. Hypertrophy of skeletal muscle in diabetic rats in response to chronic resistance exercise. J. Appl. Physiol. 87: 1075-1082, 1999. Stallknecht B, M Donsmark, LH Enevoldsen, JD Fluckey and H Galbo. Estimation of rat muscle blood flow by microdialysis perfused with ethanol, 14C-ethanol and 3H20. J. Appl. Physiol. 86: 1054-1061, 1999. Westerlind KC, JD Fluckey, SE Gordon, WJ Kraemer, PA Farrell and RT Turner. Effect of resistance exercise training on cortical and cancellous bone in mature male rats. J. Appl. Physiol. 84(2): 459-464, 1998. Brambrink JK, JD Fluckey, MS Hickey and BW Craig. Influence of mass and work on post-exercise glucose and insulin responses in young untrained subjects. Acta Physiol. Scand., 161(3): 371-377, 1997. Fluckey JD, TC Vary, LS Jefferson, WJ Evans and PA Farrell. Insulin stimulation of protein synthesis in rat skeletal muscle following resistance exercise is maintained with advancing age. J. Gerontol.: Biol. Sc., 51A, B323-B330, 1996. Fluckey JD, TC Vary, LS Jefferson and PA Farrell. Augmented insulin action on rates of protein synthesis following resistance exercise in rats. Am. J. Physiol. 270: E313-E319, 1996. Fluckey JD, WJ Kraemer and PA Farrell. Pancreatic islet insulin secretion is increased following resistance exercise in rats. J. Appl. Physiol. 79:1100-1105, 1995. Fluckey JD, MS Hickey, JK Brambrink, KK Hart, K Alexander and BW Craig. Effects of resistance exercise on glucose tolerance in normal and glucose intolerant individuals. J. Appl. Physiol. 77: 87-1092, 1994. Manuscripts in Preparation (alphabetical order additional bolded authors indicate students in my lab): Greene, NP, MI Nilsson, SF Crouse, and JD Fluckey. High Volume Resistance Exercise Enhances PPAR and PGC-1 Protein Expression, but not Markers of Mitochondrial Biogenesis in the Obese Zucker Rat. Manuscript in Review. Fluckey JD, EE Dupont-Versteegden, M Knox, MP Wiggs and CA Peterson. Amino acid trafficking for skeletal muscle protein synthesis: effect of simulated microgravity and resistance exercise. Manuscript in preparation. Fluckey JD, L Norrbrand, M Pozzo, L Smith and PATesch. Insulin is not necessary for post exercise elevations of muscle protein synthesis after 5 weeks of resistance exercise training. Presented at FASEB Conference, San Francisco, CA, 2006. Fluckey JD, MP Wiggs, CA Peterson, D Gaddy-Kurten, M Knox, L Smith-Briscoe and E Dupont-Versteegden. Amino acids derived from proteolysis are preferred for muscle protein synthesis following hindlimb suspension. [abstract], 2002, presented at FASEB conference. We have just completed follow-up studies in the MBL. Perrien DS, NS Akel, DC Montague, M Knox, JD Fluckey, EE Dupont-Versteegden, CA Peterson, L Suva, and D Gaddy. Distinct regulation of bone and muscle maintenance during hindlimb suspension by a concentric resistance exercise regimen. [abstract], 2003, submitted to BATS conference. Swift JM, F vonWalden, T Elman, JD Fluckey and SA Bloomfield. Assessing the Efficacy of Multiple Dosing Regimens of a -Agonist Agent for Bone and Muscle Loss During 28d Hindlimb Unloading. Texas ASCM, September, 2005. Wiggs MP, KP Jones, W Rayburn, T Davis, GL Dohm, and JD Fluckey. Insulin signaling in sedentary human skeletal muscle via PI3K is necessary for protein synthesis. Presented at Experimental Biology 2007, Washington DC, May 2007; Manuscript in preparation. Presentations at Regional, National or International Meetings Fluckey JD, L Norrbrand, M Pozzo, L Smith and PA Tesch. Insulin is not necessary for post exercise elevations of muscle protein synthesis after 5 weeks of resistance exercise training. Presented at FASEB Conference, San Francisco, CA, 2006. Fluckey JD, EE Dupont-Versteegden, L Smith, M Knox, P Bennett, D Gaddy and CA Peterson. The capacity of insulin to attenuate rates of protein degradation in soleus muscle is maintained in hindlimb suspended rats. Presented at FASEB Conference, San Francisco, CA, 2005. Fluckey JD, M Knox, L Smith, EE Dupont-Versteegden, D Gaddy, PA Tesch, FACSM, CA Peterson. The Insulin-facilitated increase of muscle protein synthesis after resistance exercise involves a MAP-kinase pathway. Presented at ACSM National Conference, Indianapolis, IN, 2004. Fluckey JD, EB Tapscott, RN Cortright, T Koves, S Pohnert and GL Dohm. Active involvement of PKC for insulin-mediated rates of rat muscle protein synthesis. Presented at FASEB Conference, San Diego, CA, 2003. Fluckey JD, CA Peterson, D Gaddy-Kurten, M Knox, L Smith-Briscoe and E Dupont-Versteegden. Amino acids derived from proteolysis are preferred for muscle protein synthesis following hindlimb suspension. Presented at the FASEB Conference, New Orleans, LA, 2002. Fluckey JD, S Asp, L Enevoldsen, E Richter and H Galbo. Alterations of insulin-mediated muscle protein synthesis in rats after high intensity eccentric exercise. Presented at the International Biochemistry of Exercise Meetings, Little Rock, AR, 2000. Fluckey JD, SC Pohnert, SG Boyd, RN Cortright, TA Trappe and GL Dohm. Insulin stimulation of muscle protein synthesis in obese Zucker rats is not via a rapamycin-sensitive pathway. Presented at FASEB Conference, Washington D.C., 1999. Fluckey JD, S Pohnert, G Boyd, R Cortright and GL Dohm. Obese Zucker rats exhibit insulin sensitivity with respect to rates of protein synthesis. Presented at FASEB Conference, San Fransisco, CA, 1998. Fluckey JD, T Ploug and H Galbo. Attenuated insulin action on glucose uptake and transport following resistance exercise in rats. Presented at the InterSociety Conference: Integrative Biology of Exercise, Vancouver, BC, Canada, 1996. Fluckey JD, T Ploug and H Galbo. Mechanisms associated with hypoxia- and contraction-mediated glucose transport are fiber-type dependent. Presented at the International Symposium on Exercise in Prevention, Diagnosis and Therapy of Metabolic Disorders, September, Warsaw, Poland, 1996. Fluckey JD, TC Vary, LS Jefferson, WJ Evans and PA Farrell. Insulin stimulation of protein synthesis in rat skeletal muscle following resistance exercise is maintained with advancing age. Presented at the Gerontological Societys National Conference, New Orleans, LA, 1995. Fluckey JD, TF Moccia, SE Gordon, WJ Kraemer and PA Farrell. Arginine-stimulated insulin secretion is increased following resistance exercise. Presented at the American College of Sports Medicine National Convention, Indianapolis, IN, 1994. Fluckey JD, MS Hickey, JK Brambrink, KK Hart, K Alexander and BW Craig. Effects of resistance exercise on glucose tolerance in normal and glucose intolerant individuals. Presented at the American Physiological Society - Integrative Biology Conference, Colorado Springs, CO, 1992. Co-Authored Abstracts Presented at Regional, National or International Meetings Davis AR, L Schilling, E Wellberg, B Macias, W Porter, S Riechman, JD Fluckey. Chemotherapeutic properties of myokines relased from e-stim skeletal muscle. To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Dobson JP, MI Nilsson, NP Greene, MP Wiggs, GS Latham, S Walters, and JD Fluckey. Reduced muscle mass in hindlomb muscles of obese Zucker rats is not due to suppressed protein synthesis. To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Greene NP, MI Nilsson, RO Hodlik, JD Fluckey, SF Crouse. Influence of resistance exercise on AMPK AND PGC-1 in the obese Zucker rat. To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Nilsson MI, NP Green, JP Dobson, BR Macias, MP Wiggs, HG Gasier, and JD Fluckey. Resistance exercise does not augment cumulative muscle protein synthesis in the obese Zucker rat. . To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Riechman SE, A Davis, TV Lee, V Chen, CW Lee and JD Fluckey. Skeletal muscle endocrine factors. To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Shimkus K L, MP Wiggs, MI Nilsson, JD Fluckey. Effects of acute resistance exercise on signaling markers of protein synthesis in a simulated microgravity environment. To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Wiggs MP, MI Nilsson, GS Latham, S Walters, K Flores, K Allender, JD Fluckey. Expression of Atrogin-1 is not increased in soleus or plantaris after 5 days of hindlimb unloading in rats. To be presented at the International Biochemistry of Exercise meeting, Guelph, Ontario, Canada, June 2009. Gasier HG, SF Previs, TV Lee, V Chen , MP Wiggs, SE Riechman, JD Fluckey. A comparison of 2H2O and phenylalanine flooding dose methodologies to investigate muscle protein synthesis rates in rats. Presented at the Integrative Biology of Exercise Meeting, Hilton Head, SC, Sept. 2008. Wiggs MP, HG Gasier, SF Previs, JD Fluckey. Assessment of cumulative FSR over a 24 h period with hindlimb unloading and intermittent reloading in rats. Presented at the Integrative Biology of Exercise Meeting, Hilton Head, SC, Sept. 2008. Gasier HG, MP Wiggs, JM Swift, D Oliphant, SA Riechman and JD Fluckey. Plateau effects of resistance exercise result from over-expression of eIF4E-BP1. Presented at Experimental Biology 2007, Washington DC, May 2007; Manuscript in preparation. Wiggs MP, KP Jones, W Rayburn, T Davis, GL Dohm, and JD Fluckey. Insulin signaling in sedentary human skeletal muscle via PI3K is necessary for protein synthesis. To be presented at Experimental Biology 2007, Washington DC, May 2007; Manuscript in preparation. Swift JM, F vonWalden, T Elman, JD Fluckey and SA Bloomfield. Assessing the Efficacy of Multiple Dosing Regimens of a -Agonist Agent for Bone and Muscle Loss During 28d Hindlimb Unloading. Texas ASCM, September, 2005. Mr. vonWalden and Mr. Elman were students at the Karolinska Institute under the direction of Per Tesch. Dr. Fluckey hosted these students in the MBL for specialized training to complete their theses in Stockholm. Dupont-Versteegden EE, CA Peterson, BA Strotman, M Knox, P Bennett, D Gaddy, and JD Fluckey. Resistance exercise during hind limb suspension decreases protein degradation, but not apoptosis. Integrative Biology of Exercise Conference, Austin, TX, October 2004. Knox M, EE Dupont-Versteegden, D Gaddy, CA Peterson and JD Fluckey. A novel approach to hind limb suspension in mature rats. Presented at FASEB Conference, San Diego, CA, 2003. Perrien DS, NS Akel, DC Montague, M Knox, JD Fluckey, EE Dupont-Versteegden, CA Peterson, L Suva, and D Gaddy. Distinct regulation of bone and muscle maintenance during hindlimb suspension by a concentric resistance exercise regimen. Presented at the Bone and Tooth Symposium, Oxford, England, 2003. Campbell WW, JD Fluckey, MH Morse, MD Haub. Effects of oral pinitol supplementation on oral and intravenous glucose tolerance in older people. Presented at FASEB conference, New Orleans, LA, 2002. Trappe TA, J Carrithers, SW Trappe and JD Fluckey. Skeletal muscle myosin and actin content with aging. Presented at FASEB Conference, New Orleans, LA, 2002. Trappe TA, JD Fluckey, F White, CP Lambert and WJ Evans. Skeletal muscle PGF2a and PGE2 in response to resistance exercise: Influence of ibuprofen and acetaminophen. Presented at the International Biochemistry of Exercise Meetings, Little Rock, AR, 2000. Murphy RLJ, CA Peterson, EE Dupont-Versteegden, JD Fluckey, RD Skinner and JD Houl Changes in skeletal muscle of spinal cord injured rats following passive exercise. Presented at Neuroscience National Conference, November, 1998. Cortright RN, D Zheng, JP Jones, JD Fluckey, BP Lowell and GL Dohm. The effect of denervation and exercise on UCP-3 gene expression in skeletal muscle. Presented at the Diabetes National Conference, Chicago, 1998. Pohnert S, JD Fluckey, G Boyd, M Lang and GL Dohm. Effects of thyroid hormone on rates of protein synthesis in skeletal muscle. Presented at FASEB Conference, San Fransisco, CA, 1998. Donsmark M, B Stallknecht, LH Enevoldsen, JD Fluckey and H Galbo. Skeletal muscle blood flow estimated by microdialysis probes perfused with ethanol, 14C-ethanol or 3H2O. Presented at the International Conference on the Biochemistry of Exercise, Sydney, Australia, 1997. Enevoldsen LH, B Stallknecht, JD Fluckey and H Galbo. The influence of exercise on in vivo insulin-stimulated glucose uptake in intraabdominal adipose tissue in rats. Presented at the International Conference on the Biochemistry of Exercise, Sydney, Australia, 1997. Invited Talks/Other Presentations: Co-Organizer of Mini-Symposium on Studies of Microgravity: The Need for Biomedical Research. The symposium was sponsored by the Department of Health and Kinesiology and the Sydney and JL Huffines Institute for Sports Medicine and Human Performance - 2006. The speakers included Dr. Per Tesch, Karolinska Institute, Dr. Bloomfield, Bone Biology Lab, TAMU, Dr. Fluckey, Muscle Biology Lab, TAMU and Richard Linnehan, NASA. Invited Speaker, California State University Department of Kinesiology - Chico April 6, 2006 Invited Speaker, University of Texas - Department of Kinesiology November 17, 2006 Invited Speaker, University of Kentucky College of Medicine - November 28, 2007 Invited Speaker, Texas A&M University College of Education and Human Development Advisory Council October 10, 2008 Invited Speaker, Texas Chapter American College of Sports Medicine: High intensity exercise training; new insight related to the control of muscle mass and disease prevention. Tyler, TX, February 27, 2009. Dr. Fluckey has also presented his work to groups at Texas A&M University and Scott & White Hospital. While at UAMS, Dr. Fluckey presented to a number of groups including the Departments of Geriatrics, Physiology and Biophysics, and Nutrition. He has presented this work in the North Little Rock VA, the Little Rock VA, Arknasas Childrens Hospital and UAMS. In association with his K01 award, Dr. Fluckey has also presented his work to the Departments of Physiology, Biochemistry and Exercise Science at East Carolina University, and to the Department of Physiology and Pharmacology at the Karolinska Institute in Stockhom, Sweden. Dr. Fluckey also presented at the Deans Faculty Research Retreat at UAMS in the Spring of 2003. Teaching 2008 Summer KINE 682 Skeletal Muscle Glucose Metabolism: Effect of Exercise and Disease 2008 Spring KINE 637 Exercise Physiology 2007 Fall KINE 433 Exercise Physiology 2007 Invited lecture for the Space Life Sciences Course 2007 Spring KINE 637 Graduate Exercise Physiology 2007 Spring KINE 681 Seminar Organizer 2006 Fall KINE 682 Seminar in Molecular Adaptations in Skeletal Muscle with Exercise Training 2006 Fall: KINE 433 Exercise Physiology 2006 Summer: KINE 685 - Independent Study 2006 Summer: KINE 433 Undergraduate Exercise Physiology 2006 Spring: KINE 682 Seminar in Muscle Adaptations to Microgravity 2006 Spring: KINE 637 - Invited Lecturer for Muscle Adaptations (2 sessions) in Graduate Exercise Physiology 2004 Independent Study (Spring/Fall) 2003 Independent Study (Fall) Graduate Student Journal Club Department of Physiology and Biophysics, UAMS. Acute and chronic adaptations to exercise (Spring). Graduate Student Journal Club Department of Physiology and Biophysics, UAMS. Musculoskeletal adaptations to microgravity (Fall). Medical Physiology Breakout Session Department of Physiology and Biophysics (Spring). Exercise Physiology Component. Medical Physiology Breakout Session Department of Physiology and Biophysics (Spring). Exercise Physiology Component. 1997 Summer Program for Future Doctors Department of Biochemistry, East Carolina University. Instructor: Biochemistry 1997 Advanced Exercise Physiology Department of Exercise Science, East Carolina University. Instructor. Department of Biology, Pitt Community College, Greenville, NC Instructor: Anatomy and Physiology Department of Physical Education, Clovis Community College Part-time Instructor: Principles of Weight Lifting For further information related to my performance as an instructor at Texas A&M University, undergraduate and graduate student evaluations from my classes are included in the dossier. Comprehensive Examination Committees: Jonathan Oliver (TAMU; Doctoral Candidate; Pending) Chen Chun-Wen (TAMU; Doctoral Candidate; Pending) Teak Lee (TAMU; Doctoral Candidate; Pending) Chang Woock Lee (TAMU; Doctoral Candidate; Pending) Sean Courtney (TAMU; Doctoral Candidate; Pending) Brad Lambert (TAMU; Doctoral Candidate; Pending) Julie Culbertson (TAMU; Doctoral Candidate; Passed 2010) Nicholas Greene (TAMU; Doctoral Candidate; Passed 2009) Mats Nilsson (TAMU; Doctoral Candidate; Passed - 2009) Michael Wiggs (TAMU; Doctoral Candidate; Passed - 2009) Joshua Swift (TAMU; Doctoral Candidate; Passed - 2009) Heath Gasier (TAMU; Doctoral Candidate; PASSED - 2008) Jong Hee Kim (TAMU; Doctoral Candidate; PASSED - 2008) Hyo-Bum Kwak (TAMU; Doctoral Candidate; PASSED; Defense: PASSED; Graduated in 2007) Yoonjung Park (TAMU; Doctoral Candidate; PASSED; Defense: PASSED; Graduated in 2007) Sybil Miller (TAMU; Masters Candidate; Defense: PASSED; Graduated in 2007) John Carrithers, (UAMS; Doctoral Candidate; PASSED; Graduated in 2003) Chad Carroll (UAMS; Doctoral Candidate; PASSED; Graduated in 2004) Latasha Smith (UAMS; Doctoral Candidate; PASSED; Graduation: Pending) Thesis/Dissertation Committees (excluding my students): Joshua Swift (TAMU; Doctoral Candidate; Graduated in 2010) Nicholas Green (TAMU; Doctoral Candidate; Graduated in 2010) Latasha Smith (UAMS; Doctoral Candidate; Graduated in 2009) Yoonjung Park (TAMU; Doctoral Candidate; Graduated in 2007) Hyo-Bum Kwak (TAMU; Doctoral Candidate; Graduated in 2007) Chad Carroll (UAMS; Doctoral Candidate; Graduated in 2004) John Carrithers (UAMS; Doctoral Candidate; Graduated in 2003) Rick Williams (UAMS; Masters Candidate; Graduated in 2003) Masters Comprehensive Examinations (excluding my students): David Cunningham (TAMU; PASSED; Graduated: pending) Julia Boland (TAMU; PASSED; Graduated: pending) Craig Lowrie (TAMU; PASSED; Graduated: 2010) Lauren McCreight (TAMU; PASSED; Graduated: 2010) Nathan Schwartz (TAMU; PASSED; Graduated: 2010) Grady Kaiser(TAMU; PASSED; Graduated: 2010) Kelley McGinnis (TAMU; PASSED; Graduated: 2010) Sabrina McManus (TAMU; PASSED; Graduated: 2009) Jaclyn Dominguez (TAMU; PASSED; Graduated: 2008) Gary Witty (TAMU; PASSED; Graduated: 2008) Christopher Parker (TAMU; Graduated: 2008) Erik Martinez (TAMU; Graduated: 2008) Kelsey Kinsella (TAMU at large member: NUTRITION; pending) Cassidy Kurtz (TAMU at large member: ANIMAL SCIENCE; Graduated: 2009) Current and Past Graduate Students in the MBL: Heath Gasier, MS, RD (PhD Student: Graduated 2009) Mats Nilsson (TAMU; Doctoral Candidate; Graduated 2009) Michael Wiggs, BS (PhD Student; Doctoral Candidate; Graduation: Pending) Amanda Davis, BS (PhD Student; Recipient of National Space Biomedical Research Institute Fellowship in 2007; Graduation: Pending) Justin Dobson, MS (PhD Student; Graduation: Pending) Kevin Shimkus, BS (PhD Student; Graduation: Pending) Research Dr. Fluckey has an active research program, having the privilege of currently working well-known international researchers such as G. Lynis Dohm (East Carolina University), Per A. Tesch (Karolinska Institute), Susan Bloomfield (Texas A&M University), Charlotte Peterson (University of Kentucky), Bob Wolfe (University of Texas-Medical Branch), Todd Trappe (Ball State University) and William J. Evans (University of Arkansas for Medical Sciences). Below is a brief description of grants that involve Dr. Fluckey. In addition to collaborative work with Dr. Bloomfield, Dr. Fluckey is collaborating with Drs. Riechman, E. Christou, and Crouse within the Department of Health and Kinesiology. These projects are focused on human skeletal muscle performance from whole body to subcellular perspectives. Further, these studies involve stable isotopic enrichments and percutaneous muscle biopsies for the assessment of skeletal muscle protein synthesis, and how synthesis is altered by exercise and simulated microgravity. To our knowledge, these studies are the first to obtain human muscle biopsies at Texas A&M University. Funded Research Projects Ongoing or Completed During the Last 3 Years: The effects of dietary cholesterol on skeletal muscle hypertrophy and protein synthesis Principle Investigator: Heath Gasier Mentor of Project: JD Fluckey Agency: Huffines Institute/Texas A&M University Type: Student Award/Internal The major goal of this project is to determine the effect of dietary cholesterol in skeletal muscle mass and protein synthesis, with and without resistance exercise in 6 month old, make rats. Muscle as an Endocrine Tissue Principle Investigator: JD Fluckey Agency: The Huffines Institute $ 5,000 Type: Pilot Grant Period: 01/01/08-12/31/08 Period: 01-01-08 The major goals of this research are to explore/characterize factors secreted by skeletal muscle which we have shown to have an impact on glucose metabolism during exercise. Aging and Mechanisms of Human Protein Synthesis Principle Investigator: JD Fluckey Agency: National Institute of Aging $562,109 Transferred to TAMU Type: K01 AG01025-01A1 Period: 12/01/01-11/30/07 The major goal of this research is to examine the signal transduction mechanisms of human muscle protein synthesis with advancing age.* * - Dr. Fluckey is beginning the analytical stages of this work, but we are still obtaining subjects in collaboration with Drs. Bill Rayburn and Tom Davis at Scott and White Hospital. Aside from formal data collection, this work developed an in vitro methodology to assess rates of human muscle protein synthesis with a robust activation/inhibition scheme using muscle obtained during surgery. Maintaining Musculoskeletal Health in the Lunar Environment Principle Investigator: S Bloomfield (Co-Investigator: JD Fluckey) Agency: National Space Biomedical Research Institute $1,370,018 Type: R01 Period: 06/01/08-05/31/12 The major goals of this research are to explore the effect of simulated exposure to lunar space radiation on the musculoskeletal system in mice.* * - Dr. Fluckey will examine properties of skeletal muscle physiology in simulated lunar hypogravity, as well as how it is affected by exposure to radiation and exercise countermeasures. Core C: Insulin Signaling Core Laboratory Principle Investigator: JD Fluckey Agency: National Institute of Aging $38,622 Type: Part of Program Project (P01) Period: 06/01/08-05/31/09 This core facility will conduct assays related to skeletal muscle insulin signaling in support of the overall program project. * - Dr. Fluckeys role on this Program Project is related to the area of insulin signaling and subsequent actions. Specifically, Dr. Fluckey will explore the effect of bed rest and/or intervention on specific areas of insulin signaling involved with potential protein kinase C modulation. This contribution to the program project is at the focal point of Dr. Fluckeys individual research. Dr. Fluckey is also the Core leader on this project to conduct various assays on the insulin signal transduction pathway. Currently seeking subcontract to perform work at Texas A&M University. Muscle and Bone Maintenance in Hindlimb Suspended Rats Principal Investigator: CA Peterson / Co-investigator: JD Fluckey Agency: National Institutes of Health $1,714,385 Subcontracted to TAMU Type: R01 AR47577 Period: 04/01/02-03/31/08 The major goal of this research is to perform comprehensive analysis of a novel form of resistance exercise training as a countermeasure to offset the loss of musculoskeletal structure and function after exposure to a simulated microgravity environment.* * - Dr. Fluckeys role on this grant relates to the development of the flywheel resistance exercise paradigm and assessment of rates of muscle protein synthesis and degradation with hindlimb suspension and/or resistance exercise. Since the inception of this grant, three papers have been published resulting from this work (2 first author papers and 1 second author paper), plus there have been 3 presentations at National or International Meetings. Increasing the efficiency of exercise countermeasures for bone loss Principle Investigator: S Bloomfield / Consultant: JD Fluckey Agency: National Space Biomedical Research Institute $1,788,905 Type: R01 Period: 02/01/04-01/31/08 The major goals of this research are to use pharmacology with existing countermeasures in an effort to maximize maintenance of bone in microgravity environments.* * - Dr. Fluckeys expertise with the methodology of Flywheel resistance exercise to offset losses of muscle mass incurred during microgravity environments has forged new collaborations with individuals seeking to use this methodology for their own work. Alcohol: Direct and indirect effects on drug metabolism Principle Investigator: TM Badger / Co-investigator: JD Fluckey Agency: National Institutes of Health $1,250,000 Type: R01 (renewal) Period: 10/01/04-09/30/09 This competitive renewal (project years 14-18) will to determine if altered hormone signaling or transcription factor expression during alcohol abuse affects the expression of Class I alcohol dehydrogenase.* * - Dr. Fluckeys role on this project is to conduct experiments related to insulin signaling and action on glucoregulatory function in rodents, with and without alcohol abuse. Dr. Fluckeys training in the area of metabolism, along with the methodological approaches will be a valuable asset to this project. Bedrest and Aging Principle Investigator: WJ Evans / Co-investigator: JD Fluckey Agency: National Institute of Aging $7,102,359 (overall) Type: Program Project (P01) Period: 04/01/04-03/31/09 The individual Projects are listed below. Project 2: Exercise and Muscle Principle Investigator: TA Trappe / Co-investigator: JD Fluckey Agency: National Institute of Aging $999,184 Type: Part of Program Project (P01) Period: 04/01/04-03/31/09 The major goals of this study are to examine the effect of exercise on skeletal muscle function after 10 days of bed rest in humans. Project 3: Insulin Resistance and Protein Metabolism Principle Investigator: WJ Evans / Co-investigator: JD Fluckey Agency: National Institute of Aging $987,090 Type: Part of Program Project (P01) Period: 04/01/04-03/31/09 The major goals of this study are to examine the effect of bed rest on insulin resistance and muscle protein metabolism in humans. Project 4: Tissue Lipids and Insulin Resistance Principle Investigator: RR Wolfe / Co-investigator: JD Fluckey Agency: National Institute of Aging $1,189,729 Type: Part of Program Project (P01) Period: 04/01/04-03/31/09 The major goals of this study are to examine the effect of bed rest on intramuscular lipid accumulation and oxidation, potentially leading to insulin resistance in humans. Application in submission/preparation: Effects of Dietary Protein Timing and Dietary Cholesterol on Muscle Protein Synthesis, Hypertrophy and Function following Standard or Portable Flywheel Training Principal Investigator, Program Project: Stephen Crouse, Ph.D. (submitted in 2006; was not funded and awaiting resubmission) Principal Investigator, Project Leaders: Steven E. Riechman, Ph.D., M.P.H.; James Fluckey, Ph.D.; Per Tesch, Ph.D., Karolinska Institute Agency: Dept Army Peer Reviewed Medical Research Program $1,200,000 Type: P01 Period: TBD The major goals of this research are to determine the efficacy of specialized diet and training on optimal human performance in military combat personnel. This application was initially reviewed in 2006, but was not funded. The investigative team received a comprehensive critique which should allow us to bolster the grant considerably for the next cycle. Signaling for mRNA translation: obesity and exercise Principle Investigator: JD Fluckey (First submitted: June 1, 2004; pending new submission with new preliminary data obtained while at TAMU) Agency: National Institutes of Health $ 1,025,000 Type: R01 Period: TBD The major goals of this research are to determine eIF4EBP1s role in the modulation of muscle protein synthesis and how this modulation/expression is affected by obesity and/or resistance exercise. Exercise and Breast Cancer Principle Investigator: JD Fluckey (In preparation for 2008 submission) Agency: Susan G. Komen Foundation $ 580,000 Type: Investigator-Initiated Period: TBD The major goals of this research are to explore/characterize factors secreted by skeletal muscle which we have shown to have chemotherapeutic effects on breast cancer cell lines. Other grants submitted as PI: Effects of Dietary Protein and Cholesterol and Flywheel Training on Muscle Strength, Neural Activation, Mass and Anabolic Potential during Simulated Microgravity Principle Investigator: JD Fluckey (Submitted 2007) Agency: National Space Biomedical Research Institute $1,200,000 Type: R01 Period: TBD The major goals of this research are to determine the efficacy of specialized diet and training on human performance and musculoskeletal mass with simulated space flight. Muscle proteolysis, resistance exercise and aging Principle Investigator: JD Fluckey Agency: National Institutes of Health $404,815 Type: R01 Submitted: (9/29/98) The goal of the study was to determine whether the effect of aging and resistance exercise on muscle protein degradation.* * - Dr. Fluckey altered this grant application after its second submission by moving toward human subjects. This grant is now his funded K01. Insulin, protein synthesis & resistance exercise Principle Investigator: JD Fluckey Agency: National Institutes of Health $415,419 Type: R01 Submitted: (2/25/99) The goal of the study was to systematically examine the influence of resistance exercise on insulin-mediated muscle protein synthesis.* * - Dr. Fluckeys submission of this grant application was likely premature, since strong preliminary data were not available at that time. However, over the last couple of years, Dr. Fluckey has accumulated the necessary preliminary data to once again pursue this R01 application. Project 5 of Program Project Application (Evans; PI): Immobilization and Insulin Resistance Principle Investigator: JD Fluckey Agency: National Institute of Aging $382,770 Type: Part of Program Project (P01) Period: 04/01/04-03/31/09 The major goals of this study are to examine the effect of bed rest/immobilization on insulin signaling for muscle protein synthesis and glucose tolerance.* * - Dr. Fluckeys application of the newly funded program project was not considered for funding on the basis of fit. 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Dissertation/Thesis: Insulin modulation of muscle protein synthesis after resistance exercise. (Dissertation completed at The Pennsylvania State University, 1995.) The effect of progressive resistance exercise on glucose tolerance in individuals with NIDDM. (Thesis completed at Ball State University, 1992.)     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