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Resources Biology, The Dynamics of Life, by Alton Biggs (published by Glencoe/McGraw-Hill) Tools, Equipment and Supplies Radish seeds (10 per student) Paper towels Ziploc bags (2 per student) Labels Bucket of plain water Bucket of water with fertilizer, such as Miracle Gro, mixed in Copies of Swine Finishing Lab, Seed Germination Lab and Scientific Methods Worksheet PowerPoint presentation on Scientific Method Key Terms The following terms are presented in this lesson Hypothesis Experimental Group Control Group Experiment Independent Variable Dependent Variable Interest Approach Refer to Section 2.1 in Biology: The Dynamics of Life for the details of this example of elephants. How many of you have been to a zoo recently and spent some time watching the elephants? How do they act? (Take student input) Maybe you have seen them trumpet or swing their heads or examine things with their trunks. Well, one scientist was walking through the zoo in Portland, Oregon, and felt the air throbbing around her. It was a sound she felt, more than heard, kind of like thunder. She saw that when she felt the vibrations, she also noticed the skin on the elephants head fluttering. Because of her work with whales, she began to suspect that they were communicating. So, she set up the appropriate experiments, and set out to see if she was right. Scientific experiments are all around us. Think about your own life. Im sure that each of you tends to question your parents instructions and guidance. That, in and of itself, is a scientific experiment. Heres an example. Joes parents tell him to empty the trash every day. So for a week, Joe does this. Then, he gets a little lazy and skips a day. Luckily, the trash didnt overflow, and Joe notices that his parents didnt notice or reprimand him. So, he begins to develop a hypothesis about his parents. He experiments every few days and it validates that his parents indeed dont care if he skips a day. So he revises his hypothesis and tries skipping two days. The trash overflows and Joes parents get pretty mad and dont let him take his girlfriend out on a date. And so he has learned that he can skip the trash for one day, but not two. In any experiment, there are several common steps biologists and other scientists follow. If we follow these steps, we can increase our knowledge in a valuable way. Summary of Content and Teaching Strategies Objective 1 Be able to list and explain the six major steps in scientific investigation. Look at the handout labeled, Scientific Method. Youll see a concept map that well complete as we discover the six major steps in any scientific investigation. As we talk, you should also record some important definitions in your notes. Go through the PowerPoint presentation, prompting students to record definitions and steps on their concept map handout. See the worksheet key, if needed. Try to use as many examples as possible to illustrate the concepts. Students will later use this worksheet as a reference. Objective 2 Define key investigative terms The following script uses a Me, You, Us, e- moment to provide repetition with this vocabulary. Now that weve recorded our new knowledge, lets practice it so we can commit it to memory. On your notes, I want you to think of and write down an example for these three vocabulary words: Hypothesis, independent variable, and dependent variable. Circle them on your paper so youll remember them. Ill give you about 5minutes to silently write down some brief notes about your examples. (Keep class quiet for this time, helping students who appear confused). Okay, stop where you are. When I say go, I want you to turn around in your seat and talk to one other person behind you. Share your examples and coach each other to refine your work. Ready, set, go! (Make sure everyone has a partner) Stop! Now its time to really impress me with your knowledge. Each group needs to share one of their examples with the class. It doesnt matter which definition you share, just pick your best one. (Make sure each vocabulary word is discussed and check the validity of the examples.) Objective 3 Design an experiment Please turn to your Swine Finishing Ration Lab that I handed out. This will give you an opportunity to use the information we just discussed to fill in the details of this lab. This is an activity you should complete individually, so take a few minutes to read through this and use your notes to help you complete the exercises. Assist students by using the key. After all students are done, you may collect it for a grade or review the information as a class. The final activity well do in class today is to set up our own experiment. Heres the scenario. Fred has learned that he can raise radishes to sell to the local community and earn a substantial amount towards his college fund. One farmer told him that the seeds germinate very quickly, and do best when germinated with fertilizer water. I want you to determine if that is true. Your job is to set up the experiment and get it started today. (Its ideal if this can be taught on Friday so the seeds have two days to germinate, however, some will germinate overnight if needed.) Ive given you a form that will guide you in setting up this experiment and I expect you to work either alone or quietly with one other person to do this. All the materials youll need can be found up here at the front of the room. You will stop at the end of the Design the Experiment section and well continue tomorrow. Review/Summary As we wind things up for the day, lets review some of the terms we learned. (Ask students to define the terms listed in the terms to know section. Also ask a student to review the six major steps in scientific method.) During our next class period, well continue to apply this knowledge to the world around us and look at some real examples of research. Application Extended classroom activity: Visit  HYPERLINK "http://www.accessexcellence.org/AE/mspot/croak/" http://www.accessexcellence.org/AE/mspot/croak/ and have students follow the science mystery named Croak. This is a great way to study and discuss inductive and deductive reasoning. Students take notes throughout a mystery and then solve the problem. In addition, many other science mysteries may be found at that site. FFA activity: Give each student time in class to create an experiment, conduct the experiment, and create a display for the agriscience competition at the state FFA convention. Even if the entire project wasnt created in class, it could be a great place to start a project and possibly complete it on their own time for extra credit. SAE activity: Have each student design and possibly conduct an experiment for their SAE program. The experiment could deal with the sales aspects, production, or development of new products. Evaluation This is a two-day lesson, which includes a written quiz at the end of day two. For grades or evaluation, students should turn in the Swine Finishing Ration Lab and/or Seed Germination Lab. It is up to the teachers discretion as to whether these labs are graded for completion only or specific answers. Answers to Assessment: See attached worksheet keys. Scientific Methods The Order of Investigation Scientific Methods Worksheet     Scientific Methods Worksheet Key The Order of Investigation      Swine Finishing Ration Lab Complete the details of the experiment outlined to determine the best feed for finishing hogs. Scenario: A researcher has heard from producers who say that fish meal is better than soybean meal as a protein supplement. Help design a study to determine if this is true. Recognition of the Research Problem: Write a question that explains what this experiment will study. Develop a Hypothesis: Based on the scenario, what do you think is true about the comparison of fish meal and soybean meal as a protein source for finishing hogs? Record your hypothesis here. Design the Experiment: Help make sure the experiment tests what it is supposed to test by putting a check beside each item that should be done in this experiment. _____ All the pigs should be about the same age. _____ All the pigs should be the same color. _____ There should be at least two groups. _____ There should be at least three pigs in each group to account for variability among individuals. _____ At the start of the test, the pigs should be about the same size. _____ The pigs should be assigned to groups at random. _____ The only difference in the treatment of the two groups should be the source of protein. _____ One group should be fed three times a day while the other group should be fed twice a day. _____ The pigs should all be weighed on the same day. Collect Data: Complete the table below by inserting the rest of the data. You are given the starting weights. For illustration purposes, record that all the hogs finished at 220 pounds. Complete the math so that you also record the weight gained for each hog. Group 1 Soybean Meal  Starting WeightEnding WeightWeight Gained.Pig 1 75Pig 2 76Pig 3 72Pig 4 71Total Weight Gained  Group 2 Fish Meal  Starting WeightEnding WeightWeight Gained.Pig 1 75Pig 2 76Pig 3 73Pig 4 69Total Weight Gained Analyze the Data: The weight gained by the group fed fish meal will be compared to the group fed the soybean meal. How do they compare? Draw Conclusions: What are your conclusions based on the data? Recommendations: Assuming soybean meal and fishmeal are the same price, what would you recommend to a producer as a source of protein for finishing hogs? Swine Finishing Ration Lab Key Complete the details of the experiment outlined to determine the best feed for finishing hogs. Answers are in green. Scenario: A researcher has heard from producers who say that fish meal is better than soybean meal as a protein supplement. Help design a study to determine if this is true. Recognition of the Research Problem: Write a question that explains what this experiment will study. Is fish meal better than soybean meal as a finishing ration for hogs? Develop a Hypothesis: Based on the scenario, what do you think is true about the comparison of fish meal and soybean meal as a protein source for finishing hogs? Record your hypothesis here. I believe _____________________ will be the best source of protein to finish hogs. Design the Experiment: Help make sure the experiment tests what it is supposed to test by putting a check beside each item that should be done in this experiment. __X__ All the pigs should be about the same age. _____ All the pigs should be the same color. __ X _ There should be at least two groups. __ X _ There should be at least three pigs in each group to account for variability among individuals. __ X _ At the start of the test, the pigs should be about the same size. ___ X The pigs should be assigned to groups at random. __ X _ The only difference in the treatment of the two groups should be the source of protein. _____ One group should be fed three times a day while the other group should be fed twice a day. __ X _ The pigs should all be weighed on the same day. Collect Data: Complete the table below by inserting the rest of the data. You are given the starting weights. For illustration purposes, record that all the hogs finished at 220 pounds. Complete the math so that you also record the weight gained for each hog. Group 1 Soybean Meal  Starting WeightEnding WeightWeight Gained.Pig 1 75220145Pig 2 76220144Pig 3 72220148Pig 4 71220149Total Weight Gained586  Group 2 Fish Meal  Starting WeightEnding WeightWeight Gained.Pig 1 75220145Pig 2 76220144Pig 3 73220147Pig 4 69220151Total Weight Gained587 Analyze the Data: The weight gained by the group fed fish meal will be compared to the group fed the soybean meal. How do they compare? The group fed fishmeal gained slightly more weight that the group fed soybean meal, however, the difference was only 1 pound. Draw Conclusions: What are your conclusions based on the data? Fishmeal is a slightly better product for finishing market hogs. Recommendations: Assuming soybean meal and fish meal are the same price, what would you recommend to a producer as a source of protein for finishing hogs? I would recommend fishmeal, however, more experimentation data may be needed. Seed Germination Lab Name _________________________________ Date _________________________ Observations: Fred has learned that he can raise radishes to sell to the local community and earn a substantial amount toward his college fund. One farmer told him that the seeds germinate very quickly, and do best when germinated with fertilizer water. Your job is to determine if that is true. Research Problem: Write one question that sums up the information you are trying to discover. Hypothesis: What is your hypothesis (1-3 full sentences) Design the Experiment: Collect 10 radish seeds, two paper towels, two Ziploc bags, 2 labels, a small container of water and a small container of fertilizer water. Dampen one paper towel in the water, place five seeds in the middle of the paper towel, and then fold the papertowel over the seeds and place in the Ziploc bag. Seal it so there is no air inside. Label this bag, Radish seeds in water, along with your name and the date. Now, follow the same procedure with the other five seeds in a paper towel dampened with fertilizer water. Label it appropriately as well. What is the dependent variable? What is the independent variable? Collect and Analyze Data: After leaving the seeds in a moderately warm place overnight or over a weekend, unwrap the seeds and record how many have germinated on the data table below. Solution Number of seeds germinated Pure Water  Fertilizer WaterDraw a small graph below that illustrates these results. Remember to label the units. Conclusions and Recommendations: In 3-5 full sentences, describe what you can conclude form these results. Did water with fertilizer help the seeds germinate faster? Does this experiment create any other questions for which experimentation is needed? What do you recommend Fred use in his radish production enterprise?     PAGE  PAGE 1 Unit 1, Lesson 6: A Look at Livestock: Scientific Investigation Part 1 Important Definitions: Hypothesis: Deductive Reasoning: Inductive Reasoning: Independent Variable: Dependent Variable: Control Group: Experimental Group:  Have independent and dependent variables Use inductive or deductive reasoning Conclusions and Recommendations Collect & Analyze Data Design the Experiment Form a Hypothesis Recognize Research Problem Important Definitions: Hypothesis: A testable explanation for a question or problem. Deductive Reasoning: Suggesting that something might be true about a specific case from known general rules. It is often expressed as an If . . . then . . . statement. Inductive Reasoning: Reasoning from a particular set of facts to a general rule. Independent Variable: the condition being changed. Dependent Variable: the condition that results from the change. Control Group: the standard group, all conditions kept the same. 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